LOCUS       NM_018086               9537 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens fidgetin, microtubule severing factor (FIGN),
            transcript variant 1, mRNA.
ACCESSION   NM_018086
VERSION     NM_018086.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Qi,L., Chen,S., Liao,Z., Fan,M., Zhang,J., Gao,Y., Shen,J., Sun,Y.
            and Wang,Q.
  TITLE     Comprehensive characterization of Fidgetin on tumor immune
            microenvironment evaluation and immunotherapy in human
            hepatocellular carcinoma
  JOURNAL   Aging (Albany NY) 16 (5), 4445-4468 (2024)
   PUBMED   38421251
  REMARK    GeneRIF: Comprehensive characterization of Fidgetin on tumor immune
            microenvironment evaluation and immunotherapy in human
            hepatocellular carcinoma.
REFERENCE   2  (bases 1 to 9537)
  AUTHORS   Zhou,B., Wang,J., Gao,J., Xie,J. and Chen,Y.
  TITLE     Fidgetin as a potential prognostic biomarker for hepatocellular
            carcinoma
  JOURNAL   Int J Med Sci 17 (17), 2888-2894 (2020)
   PUBMED   33162817
  REMARK    GeneRIF: Fidgetin as a potential prognostic biomarker for
            hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9537)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 9537)
  AUTHORS   Wang,D., Chu,M., Wang,F., Zhou,A., Ruan,M. and Chen,Y.
  TITLE     A Genetic Variant in FIGN Gene Reduces the Risk of Congenital Heart
            Disease in Han Chinese Populations
  JOURNAL   Pediatr Cardiol 38 (6), 1169-1174 (2017)
   PUBMED   28534241
  REMARK    GeneRIF: FIGN might play an important role in decreased congenital
            heart defect risk by upregulating plasma folate concentration
            during embryo heart development
REFERENCE   5  (bases 1 to 9537)
  AUTHORS   Wang,D., Wang,F., Shi,K.H., Tao,H., Li,Y., Zhao,R., Lu,H., Duan,W.,
            Qiao,B., Zhao,S.M., Wang,H. and Zhao,J.Y.
  TITLE     Lower Circulating Folate Induced by a Fidgetin Intronic Variant Is
            Associated With Reduced Congenital Heart Disease Susceptibility
  JOURNAL   Circulation 135 (18), 1733-1748 (2017)
   PUBMED   28302752
  REMARK    GeneRIF: FIGN +94762G>C polymorphism reduced the risk of congenital
            heart defects by activating the transcription of an alternative
            FIGN isoform, inhibiting proteasome activity and allowing the
            accumulation of RFC1 and DHFR, promoting folate transmembrane
            transport and utilization.
REFERENCE   6  (bases 1 to 9537)
  AUTHORS   Hazra,A., Kraft,P., Lazarus,R., Chen,C., Chanock,S.J., Jacques,P.,
            Selhub,J. and Hunter,D.J.
  TITLE     Genome-wide significant predictors of metabolites in the one-carbon
            metabolism pathway
  JOURNAL   Hum Mol Genet 18 (23), 4677-4687 (2009)
   PUBMED   19744961
REFERENCE   7  (bases 1 to 9537)
  AUTHORS   Talmud,P.J., Drenos,F., Shah,S., Shah,T., Palmen,J., Verzilli,C.,
            Gaunt,T.R., Pallas,J., Lovering,R., Li,K., Casas,J.P., Sofat,R.,
            Kumari,M., Rodriguez,S., Johnson,T., Newhouse,S.J., Dominiczak,A.,
            Samani,N.J., Caulfield,M., Sever,P., Stanton,A., Shields,D.C.,
            Padmanabhan,S., Melander,O., Hastie,C., Delles,C., Ebrahim,S.,
            Marmot,M.G., Smith,G.D., Lawlor,D.A., Munroe,P.B., Day,I.N.,
            Kivimaki,M., Whittaker,J., Humphries,S.E. and Hingorani,A.D.
  CONSRTM   ASCOT investigators; NORDIL investigators; BRIGHT Consortium
  TITLE     Gene-centric association signals for lipids and apolipoproteins
            identified via the HumanCVD BeadChip
  JOURNAL   Am J Hum Genet 85 (5), 628-642 (2009)
   PUBMED   19913121
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 9537)
  AUTHORS   Alkelai,A., Greenbaum,L., Rigbi,A., Kanyas,K. and Lerer,B.
  TITLE     Genome-wide association study of antipsychotic-induced parkinsonism
            severity among schizophrenia patients
  JOURNAL   Psychopharmacology (Berl) 206 (3), 491-499 (2009)
   PUBMED   19680635
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
REFERENCE   9  (bases 1 to 9537)
  AUTHORS   Trynka,G., Zhernakova,A., Romanos,J., Franke,L., Hunt,K.A.,
            Turner,G., Bruinenberg,M., Heap,G.A., Platteel,M., Ryan,A.W., de
            Kovel,C., Holmes,G.K., Howdle,P.D., Walters,J.R., Sanders,D.S.,
            Mulder,C.J., Mearin,M.L., Verbeek,W.H., Trimble,V., Stevens,F.M.,
            Kelleher,D., Barisani,D., Bardella,M.T., McManus,R., van Heel,D.A.
            and Wijmenga,C.
  TITLE     Coeliac disease-associated risk variants in TNFAIP3 and REL
            implicate altered NF-kappaB signalling
  JOURNAL   Gut 58 (8), 1078-1083 (2009)
   PUBMED   19240061
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   10 (bases 1 to 9537)
  AUTHORS   Cox,G.A., Mahaffey,C.L., Nystuen,A., Letts,V.A. and Frankel,W.N.
  TITLE     The mouse fidgetin gene defines a new role for AAA family proteins
            in mammalian development
  JOURNAL   Nat Genet 26 (2), 198-202 (2000)
   PUBMED   11017077
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC092684.2, AK125324.1, BX649105.1 and AC093727.3.
            
            On Nov 22, 2018 this sequence version replaced NM_018086.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments, including
            RNA-seq data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DA853548.1, SRR1660807.220894.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000333129.4/ ENSP00000333836.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AC092684.2         59854-59854         c
            2-5                 AK125324.1         2-5
            6-2133              BX649105.1         3-2130
            2134-9537           AC093727.3         1513-8916           c
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q24.3"
     gene            1..9537
                     /gene="FIGN"
                     /note="fidgetin, microtubule severing factor"
                     /db_xref="GeneID:55137"
                     /db_xref="HGNC:HGNC:13285"
                     /db_xref="MIM:605295"
     exon            1..171
                     /gene="FIGN"
                     /inference="alignment:Splign:2.1.0"
     exon            172..341
                     /gene="FIGN"
                     /inference="alignment:Splign:2.1.0"
     CDS             317..2596
                     /gene="FIGN"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="fidgetin isoform 1"
                     /protein_id="NP_060556.2"
                     /db_xref="CCDS:CCDS2221.2"
                     /db_xref="GeneID:55137"
                     /db_xref="HGNC:HGNC:13285"
                     /db_xref="MIM:605295"
                     /translation="MISSTSVYGLKMQWTPEHAQWPEQHFDITSTTRSPAHKVEAYRG
                     HLQRTYQYAWANDDISALTASNLLKKYAEKYSGILEGPVDRPVLSNYSDTPSGLVNGR
                     KNESEPWQPSLNSEAVYPMNCVPDVITASKAGVSSALPPADVSASIGSSPGVASNLTE
                     PSYSSSTCGSHTVPSLHAGLPSQEYAPGYNGSYLHSTYSSQPAPALPSPHPSPLHSSG
                     LLQPPPPPPPPPALVPGYNGTSNLSSYSYPSASYPPQTAVGSGYSPGGAPPPPSAYLP
                     SGIPAPTPLPPTTVPGYTYQGHGLTPIAPSALTNSSASSLKRKAFYMAGQGDMDSSYG
                     NYSYGQQRSTQSPMYRMPDNSISNTNRGNGFDRSAETSSLAFKPTKQLMSSEQQRKFS
                     SQSSRALTPPSYSTAKNSLGSRSSESFGKYTSPVMSEHGDEHRQLLSHPMQGPGLRAA
                     TSSNHSVDEQLKNTDTHLIDLVTNEIITQGPPVDWNDIAGLDLVKAVIKEEVLWPVLR
                     SDAFSGLTALPRSILLFGPRGTGKTLLGRCIASQLGATFFKIAGSGLVAKWLGEAEKI
                     IHASFLVARCRQPSVIFVSDIDMLLSSQVNEEHSPVSRMRTEFLMQLDTVLTSAEDQI
                     VVICATSKPEEIDESLRRYFMKRLLIPLPDSTARHQIIVQLLSQHNYCLNDKEFALLV
                     QRTEGFSGLDVAHLCQEAVVGPLHAMPATDLSAIMPSQLRPVTYQDFENAFCKIQPSI
                     SQKELDMYVEWNKMFGCSQ"
     exon            342..9537
                     /gene="FIGN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4512..4517
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FIGN"
                     /note="hexamer: AATAAA"
     polyA_site      4535
                     /gene="FIGN"
     regulatory      9510..9515
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FIGN"
                     /note="hexamer: AATAAA"
     polyA_site      9537
                     /gene="FIGN"
                     /note="major polyA site"
ORIGIN      
        1 gttttgggtt tgaaattcca acatggcaga ggctgcagtc cgtcttccct tcaaaaactt
       61 ggaatgattt caaatcatag gcaccttcac ttaaccctag cttccattca tcagcaaaca
      121 catcggatcg atgctacgct aacctatcgg gttctctctc cgcgcgttca ggttaaatga
      181 atacctgacg aaagggccca cgtttcaagg cagtgacatt tgatagctga gaggaaaagt
      241 ggctttaatg aaaagcaacc tttggaattc ctgcttgtga gaaatccaat tcagcttttt
      301 gtgctgccag caagaaatga tcagtagcac cagtgtttat ggcttgaaga tgcagtggac
      361 gccagagcat gcccagtggc cagaacagca ctttgacatc acctcaacca ctcggtctcc
      421 tgcccacaaa gttgaagcct acagaggtca tctgcagcgc acctatcagt acgcctgggc
      481 gaatgatgac atatctgctc tgactgcatc caacctacta aaaaaatatg cagagaagta
      541 ttccggcatt ttggaaggtc ctgtggaccg acccgtactc agcaactatt cggacacacc
      601 atcaggacta gtgaacggtc ggaaaaatga aagtgaaccc tggcagcctt ccttgaattc
      661 agaagctgtt tatcccatga actgtgttcc ggatgttatc actgccagca aagctggagt
      721 cagttcagcc ctccctccag cagatgtctc tgcgagtata ggaagctctc ctggggtagc
      781 cagcaacctg acagaaccta gttattcaag tagtacctgt ggaagccaca ctgtacccag
      841 tcttcatgca gggctcccat ctcaggaata tgccccagga tacaacggat catatttgca
      901 ttctacttat agtagccagc cagcacctgc acttccttca cctcatccgt ctcctttgca
      961 tagctctggg ctactacagc ccccaccacc acctcctccg ccaccagcct tggtcccagg
     1021 ctacaatggg acttctaacc tctccagtta cagctatccg tctgctagct atcctcctca
     1081 gactgctgtg gggtctgggt acagccctgg gggggcaccg cctccgcctt cagcgtacct
     1141 gccttcagga attcctgctc ccacccccct accccccacc actgttcctg gctacaccta
     1201 ccagggccat ggtttgacac ctattgcacc gtcggctctg acaaacagtt cagcaagttc
     1261 tctcaaaagg aaagctttct acatggcagg gcaaggagat atggactcca gttatggaaa
     1321 ttacagctat ggccaacaga gatctacaca gagtcctatg tacagaatgc ccgacaacag
     1381 catttcaaac acaaatcggg ggaatggctt tgacagaagt gctgaaacat catccttagc
     1441 atttaagcca acgaagcagc taatgtcctc tgaacagcaa aggaaattca gcagccagtc
     1501 cagtagggct ctgacccctc cttcctacag tactgctaaa aattcattgg gatcaagatc
     1561 cagtgaatcc tttgggaagt acacatcgcc agtaatgagt gagcatgggg acgagcacag
     1621 gcagctcctc tctcacccaa tgcaaggccc tggactccgt gcagctacct catccaacca
     1681 ctctgtggac gagcaactga agaatactga cacgcacctc atcgacctgg taaccaatga
     1741 gattatcacc caaggacctc cagtggactg gaatgacatt gctggtctcg acctggtgaa
     1801 ggctgtcatt aaagaggagg ttttatggcc agtgttgagg tcagacgcgt tcagtggact
     1861 gacggcctta cctcggagca tccttttatt tggacctcgg gggacaggca aaacattatt
     1921 gggcagatgc atcgctagtc agctgggggc cacatttttc aaaattgccg gttctggact
     1981 agtcgccaag tggttaggag aagcagagaa aattatccat gcctcttttc ttgtggccag
     2041 gtgtcgccag ccctcggtga tttttgttag tgacattgac atgcttctct cctctcaagt
     2101 gaatgaggaa catagtccag tcagtcggat gagaaccgaa tttctgatgc aactggacac
     2161 tgtactaact tcggctgagg accaaatcgt agtaatttgt gccaccagta aaccagaaga
     2221 aatagatgaa tcccttcgga ggtacttcat gaaacgactt ttaatcccac ttcctgacag
     2281 cacagcgagg caccagataa tagtacaact gctctcacag cacaattact gtctcaatga
     2341 caaggagttt gcactgctcg tccagcgcac agaaggcttt tctggactag atgtggctca
     2401 tttgtgtcag gaagcagtgg tgggccccct ccatgccatg ccagccacag acctttcagc
     2461 cattatgccc agccagttga ggcccgttac atatcaagac tttgaaaatg ctttctgcaa
     2521 gattcagcct agcatatctc aaaaggagct tgatatgtat gttgaatgga acaaaatgtt
     2581 tggttgcagt cagtgataac ttctttagaa aaaaaaaatg taatgaatgt tggcacacac
     2641 acataaaacc tgctacatag ggaatagagc ccctttccag tagagtttaa attgcaaagg
     2701 gtactgggga agatgacgat taagttgcat ctttagagtc agggtagatt tggaggaaaa
     2761 gtgcatcaaa tgagagcttc tgatttgaaa gccccagatg acagaaagca tatgtggatg
     2821 ctcagttctg ttcaagctag acaacactca ccaaggagca aggtgcaagt gtgttgattt
     2881 cagaaggaca tgaacctcgt gtgttgattc cattctgctg ttctcgagat ttagttgctg
     2941 tcaagtgcct ggagtggtgc tttatttttt gtttgcctca caattacatt ggtggcatgt
     3001 gctaatataa agagctttaa cttcaaacat tattggacta aagagatgaa cagttgtgtt
     3061 atgacagaaa accagatttt tgccatttta agagcaacag tattcctcaa tcctgtctgt
     3121 tctgcagtat taagctaaga acaggtaaaa cagggtaacg gtaatctgga ccttaatttc
     3181 tgcagttcat ttcttttaat gttcttgtct gcaaaaactc aggaaagtga ttgtgatttg
     3241 tacagtacct caaaggaatg tgttgaaagc actatgtact gctgagagta ataggatagg
     3301 cttcaatgtt actttatatt aaaatgtatg tttacctcaa caattggaaa atagcaagga
     3361 aaattacttt gaatgtatcc agaaaaatac tgaagtgtga tacaactgaa tatttacagt
     3421 ttaaagtaga aatggaagga tttttttaag ttcttttact aattatgggg aattaaccag
     3481 agcagaataa ttctttatgt caataactgc aagagttctt agtacattgc tccttgataa
     3541 ttaagtgaaa atgttcttaa aaggtacact ggttaattga aagctactta ttcagtttgt
     3601 gttagtgtct agacctgtca gccacaagac ctgtttagga ccctgaaagt cacagtacct
     3661 aaaaactatg actgcctttt tattgcatag gtggtagtgg tggtgatggt ggtggtagtt
     3721 tgcaagttat ctcttaaaac tgctgggaat ggtgtcattc tattcactaa tctagcttat
     3781 agacttgccg tgctgtttga tagaatgcag aggatagcaa ccaaaacaaa tacacaaata
     3841 aataaaaaca aaaaccaacc aacaaaccaa cttacataca catatatata tccacaaaga
     3901 acctctccat ctcctcccct tctttttgac tccactcttg tcagtgcaat tttgcttctc
     3961 attttgaaat ctgggctgta gtgctcctgc tttatttcta cctcagtttt gttacatttc
     4021 tcttggaaag taaagtagaa aattggaagt ggacacacac actgcaatgt agcttgccaa
     4081 acatgttact ttgttttctt ccatctttca ccgtaaatct agtttccaaa gacatcagca
     4141 tttgtgctta cttccacctc agtctaccag ccccacccct acccatggca taagtggcat
     4201 ttttcttaat ttcctatttt tctcctgctc tctgtcaagt tgttctttgt atcctttaat
     4261 gctttatgtg caacctttca ttgatagtgg gctgatgttt ggcaatgctt ctgaactgtc
     4321 acagagcagg ctgtagcttt ccacagccac tgcccatgca taagcagaac agcctggcct
     4381 tttgaatgta ttttcctggg ttttttcccc ttttcttttt ttagtttaga gatgcagtaa
     4441 caaaactgtt gcaaagcact ggcattttat gtattcaata aataagtgat gtacattttt
     4501 aaaaaaattt aaataaatgc aatgagaagc cccaagaaag gcctttctgt tttttttttc
     4561 ttacaaaaat ttttaccgat cttatccaca atatccaatt ctctgaaaca cttaacatag
     4621 agaaagctca tgagggattt cttatctctg aaattttatg atgcttgatc tttactcccc
     4681 tttttttatt ttttatgttg ctaaaaactg agcaattgaa ccagaattta atttactgtg
     4741 acattctgtc aattaaagct gtgcttaaat ataaatattc catattaaaa agcagtgcct
     4801 ttcattaagg atacaaaaaa taatacttaa atacgccctc tgcttttcgc aagtgaataa
     4861 atgctacaat cgtgttctgg gtctaccctg tgcatccctt gtaaggaaat tctaatatcc
     4921 acattaactt gtaggttcat attgatctca ctgacaagtt tacttttgca cagtgctgac
     4981 ctattttctg acttttagac attatatttc atttccgcca acatgctgtg ttcacttgaa
     5041 agcgtctggc aagttttgac aaacccagtc ttatttagac aactttggaa aatccaatat
     5101 tgacccacat ttttttgtgg ctagtaagat ttaatgccaa atatatgact gcactattgt
     5161 gttttacact catttataaa actgaaaata aattgaagtc atacatcttg gcttttagct
     5221 ttaagtgact taaaaattag aactctgagt gacttaaaaa ttaatttgag atgcaagtgg
     5281 tcaaaaatcc atgtatttag acagaagctg aagtcgtatg tgattttcat ttagcctcac
     5341 aaaatccaag atttgatctc ttaggaaatt gggcatgttg aagaaatgaa gatctgttgg
     5401 aggagatatg gctgaccttt gagtagatag tgctctcctg tgattctttg attctgcatg
     5461 gcagcattat gagctcttct ataaactagc ttaataatga gctctattct gaccatctat
     5521 tctggaactt ggagtggggg tggggtacca catcctcaga agtgaatgtg tacagtcggt
     5581 gatagtacaa ctgagtaatg gggcttcata cccttggcct ctttttgact gctaagaatt
     5641 tggagaaggc tattaatgaa ggtaaggtaa tgagccagtc caaatccctc actgtctggc
     5701 taagagattg agccattcca ctttacagcc cctaattccc ttcttcccaa ctgcagcaac
     5761 atttccaaca gggacaaaaa ggaagtgaag gaaaccactt cagtattggg atcaatagaa
     5821 attcaagagg tagttcataa tgaccttata tgtgacacaa atggaaatac tctacacttc
     5881 tggcatgctt ctctcccaaa tatttagaac attagtgaca atatgaaaaa ctttgctgac
     5941 tgagctgatc aatggaatgt gaaggcagta tactttaagg ctatttactt tcacacagaa
     6001 tgtttatcta ttagtcagtt tgtattctac tcaaatgcag atagacgggt ataatgtttt
     6061 cttgtgcatt tgaaacctat caaggagaaa ataaaccatt ccactaaaat acataggcca
     6121 aacaccatct gggaaagtga taaaagctat ccagaggcta tgctatgtaa aatagcaagg
     6181 tcaatttaca tgggttgcat agtacttggc tatgcagttt tcaaaagaac acatccaaaa
     6241 aaaaaaaaaa tccaaattag ttcctataat aggtctttgt aaatttaaat atatttgtgg
     6301 gttggaaaat acgtttttag agacatttag aatttctttc tctaaaaatg tatccaactc
     6361 caagcagaaa gagacttctg tactcacatt ctcatgtttc ttagaattgg gtagcatgat
     6421 cattgaatct gtattttgca aagtgaaagg gacgcagctt gttgaactat tgaggccact
     6481 aaagtaaagt tctaatatat atatataaat ataaaattag tgtgtgtgtt tgtatgtgtg
     6541 tatgtgtgtg tgttctttca tcaccaccta gttgaacttt aaaacagacg tctacttttc
     6601 attattcaga agcaacttgt tacttccttt cttcatatgt gcagagatac acatgatcat
     6661 gttacaattt gaagtgtttt aatgtgtttt tttttctttt gaaacgtaaa gccttcagtt
     6721 gttgaatcat ctgccgattt ttatatgaag cacattagca aacctggagc tattagtatt
     6781 tcagaattca caaccagctg tacttgtgac taaccaacac atgaactgaa gctcacactt
     6841 tgctaagacc tttggttgac attcgatttt gaacctgcca gatgcatttg tgtgaaccag
     6901 ggatactgca tctttaatga acaagtctct cctctttccc ctgtttttaa atacaacaca
     6961 agcagtgtag cactaggcac tgcaattgtc aaagacaatt agactggaag caggaaaatt
     7021 tcacctccct gtctcagtga tatgtagaat gtcggcagca tgggtcagcc ttgccacaga
     7081 gttgtgtgag aatatatgaa tcagtcctat agaatgttaa atatttataa aatggttctg
     7141 tagtacacca aggttgggag cacattcatt ccttcttgtt gtcttatgat acaaaaaaaa
     7201 aaaaaaaaaa agcaaaagtt tatttctggg cttaaaactc tccctctaat atctctctct
     7261 cttttacccc cattccccaa ccattcttct ctccatccct ctcctctcct cttcctctct
     7321 cttctctctc tctctctctc tctctgtctc taactctcgc tctctcttgt atgtgggtgt
     7381 gtgtgtgtat gagtttccac tgtggattat atacacttgc tagagaatat tcatttcttt
     7441 tttttctttt ttcatctgat gtatattcaa acattgttct ttataaccat ttctacctca
     7501 ttgctacata ttgtacatgt agtatttatt tgttgtcttt ttttgaatgt catgcatttc
     7561 gtaagaaaaa gacttgtcct tgaacttgaa cagtctacct cagaaagact gtctttacac
     7621 tgaacagtat tagcaaccta aatgataaga cgcattagtg aagtgtggca gggtagataa
     7681 tgcacgagta cttgggatac tgatggactt ttaattgtat tcataacaca agactgctta
     7741 aaagaactta aaagaaatta tgcactgtgt aggtcttcat cgaggtccca tttgggacat
     7801 tatatacaga atagagcttt attcctatgt gcccatctct gagaaatgtg ttagcattcc
     7861 caagtatttt acactggcaa tctggctact taagacaaac aaattcaaac aacattttta
     7921 tattttgact gacaccctca gctcctaaat ttaagagaaa taatggtact taactatttt
     7981 caactatagt cttcagtgat tgaaaatgcc ttcattttaa ttctgtgact cctgatataa
     8041 attaagaaaa acaatgcagt tgcaagggcc tgcacaacac tgaaaataaa ccaaaaatta
     8101 aaaatggcac ttcccaaagg aattaacaaa cgtggagtca tggtgcttgc aagttccata
     8161 actgaaagca ttttgtttct gatttccctt tataacatgt atttgtttat tgaagcaatt
     8221 gtacaactaa tggcacattg aattactttg tatgaagtgt tttatattat actatttatt
     8281 ttagcattcg ttctttttct tcaccagctc tatattacag tagcaaaaga acacttagtt
     8341 ttaaaaaatg ctgaaagggg ttcaccaccc acccaatcga tgaatggact tttgaagttg
     8401 cttgtcagca aattgtggat attttacaaa agcaaatgaa ctgtgacaat ccacaactcc
     8461 cacagatgtg aattcagaaa tgcatctgaa actccggtat gcctgacaat ctattcttta
     8521 agtttcatta acaaagtggc atccaatcct ttcacttaca attccttgca ggaatgtatt
     8581 gtctagaggc tgtctagtag tgattaagag agggtttttg ttttgtttta tttgtttact
     8641 gtattaggtg aggaatgcca tatttcttgt cagccaacag tacttgtctg agacagaaat
     8701 ttaaaaaaag aaaaagaaac ctaactaatt aaaatttctg tgcactttgc ttccctgacc
     8761 atattgattt tatttgctta aattgaaaca atacgctgga tttgaccaga ttttgacccc
     8821 ttgaaagaag aaaagcccta tatagtacat gtttctttgg gttattctag tactgtttcc
     8881 ttatttgttc tgcaaatttc aaatcatttt tttgtgtgca ttattttaca tctgctgaaa
     8941 ctaaatgtgt tattattgca ctcattaaaa atgaaacaat ccatttctct tggaaaaaaa
     9001 tgacaagctt tagatgatga aagcttaaat tatccatcac ttgcaaaatg aattcatata
     9061 atttgtgaac attattaatg taaatgagac atttctgctt agattttttt tattttgatg
     9121 ggatcattat atggttgatc atatgtgtgt aggaagctgc tgtacaatta acttggagat
     9181 ctgaaaatgc tttttgtcct cattgttgca gtttcaattg taatccattg catctcattt
     9241 tcttgggtgt tggcactgta atatattaaa aaaaaattca gtgctcagta ttgtaacaac
     9301 tgtccctact gaatattcct tttcataaat acttgctacc acagggaaat taagttttca
     9361 tatagaataa ctagtttcag tagtaaccat tgaagaatta aaaatgtggt tcaaggcaga
     9421 tatttttatg aaaagttttc tctattgtaa aatttgttgt atatggctat gctactatca
     9481 tggaataaga aaagaacaag catacctcaa ataaaaaaaa attctgagtt aaaaaaa
//
