LOCUS       NM_001321825            9367 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens fidgetin, microtubule severing factor (FIGN),
            transcript variant 2, mRNA.
ACCESSION   NM_001321825 XM_005246662
VERSION     NM_001321825.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Qi,L., Chen,S., Liao,Z., Fan,M., Zhang,J., Gao,Y., Shen,J., Sun,Y.
            and Wang,Q.
  TITLE     Comprehensive characterization of Fidgetin on tumor immune
            microenvironment evaluation and immunotherapy in human
            hepatocellular carcinoma
  JOURNAL   Aging (Albany NY) 16 (5), 4445-4468 (2024)
   PUBMED   38421251
  REMARK    GeneRIF: Comprehensive characterization of Fidgetin on tumor immune
            microenvironment evaluation and immunotherapy in human
            hepatocellular carcinoma.
REFERENCE   2  (bases 1 to 9367)
  AUTHORS   Zhou,B., Wang,J., Gao,J., Xie,J. and Chen,Y.
  TITLE     Fidgetin as a potential prognostic biomarker for hepatocellular
            carcinoma
  JOURNAL   Int J Med Sci 17 (17), 2888-2894 (2020)
   PUBMED   33162817
  REMARK    GeneRIF: Fidgetin as a potential prognostic biomarker for
            hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9367)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 9367)
  AUTHORS   Wang,D., Chu,M., Wang,F., Zhou,A., Ruan,M. and Chen,Y.
  TITLE     A Genetic Variant in FIGN Gene Reduces the Risk of Congenital Heart
            Disease in Han Chinese Populations
  JOURNAL   Pediatr Cardiol 38 (6), 1169-1174 (2017)
   PUBMED   28534241
  REMARK    GeneRIF: FIGN might play an important role in decreased congenital
            heart defect risk by upregulating plasma folate concentration
            during embryo heart development
REFERENCE   5  (bases 1 to 9367)
  AUTHORS   Wang,D., Wang,F., Shi,K.H., Tao,H., Li,Y., Zhao,R., Lu,H., Duan,W.,
            Qiao,B., Zhao,S.M., Wang,H. and Zhao,J.Y.
  TITLE     Lower Circulating Folate Induced by a Fidgetin Intronic Variant Is
            Associated With Reduced Congenital Heart Disease Susceptibility
  JOURNAL   Circulation 135 (18), 1733-1748 (2017)
   PUBMED   28302752
  REMARK    GeneRIF: FIGN +94762G>C polymorphism reduced the risk of congenital
            heart defects by activating the transcription of an alternative
            FIGN isoform, inhibiting proteasome activity and allowing the
            accumulation of RFC1 and DHFR, promoting folate transmembrane
            transport and utilization.
REFERENCE   6  (bases 1 to 9367)
  AUTHORS   Hazra,A., Kraft,P., Lazarus,R., Chen,C., Chanock,S.J., Jacques,P.,
            Selhub,J. and Hunter,D.J.
  TITLE     Genome-wide significant predictors of metabolites in the one-carbon
            metabolism pathway
  JOURNAL   Hum Mol Genet 18 (23), 4677-4687 (2009)
   PUBMED   19744961
REFERENCE   7  (bases 1 to 9367)
  AUTHORS   Talmud,P.J., Drenos,F., Shah,S., Shah,T., Palmen,J., Verzilli,C.,
            Gaunt,T.R., Pallas,J., Lovering,R., Li,K., Casas,J.P., Sofat,R.,
            Kumari,M., Rodriguez,S., Johnson,T., Newhouse,S.J., Dominiczak,A.,
            Samani,N.J., Caulfield,M., Sever,P., Stanton,A., Shields,D.C.,
            Padmanabhan,S., Melander,O., Hastie,C., Delles,C., Ebrahim,S.,
            Marmot,M.G., Smith,G.D., Lawlor,D.A., Munroe,P.B., Day,I.N.,
            Kivimaki,M., Whittaker,J., Humphries,S.E. and Hingorani,A.D.
  CONSRTM   ASCOT investigators; NORDIL investigators; BRIGHT Consortium
  TITLE     Gene-centric association signals for lipids and apolipoproteins
            identified via the HumanCVD BeadChip
  JOURNAL   Am J Hum Genet 85 (5), 628-642 (2009)
   PUBMED   19913121
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 9367)
  AUTHORS   Alkelai,A., Greenbaum,L., Rigbi,A., Kanyas,K. and Lerer,B.
  TITLE     Genome-wide association study of antipsychotic-induced parkinsonism
            severity among schizophrenia patients
  JOURNAL   Psychopharmacology (Berl) 206 (3), 491-499 (2009)
   PUBMED   19680635
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
REFERENCE   9  (bases 1 to 9367)
  AUTHORS   Trynka,G., Zhernakova,A., Romanos,J., Franke,L., Hunt,K.A.,
            Turner,G., Bruinenberg,M., Heap,G.A., Platteel,M., Ryan,A.W., de
            Kovel,C., Holmes,G.K., Howdle,P.D., Walters,J.R., Sanders,D.S.,
            Mulder,C.J., Mearin,M.L., Verbeek,W.H., Trimble,V., Stevens,F.M.,
            Kelleher,D., Barisani,D., Bardella,M.T., McManus,R., van Heel,D.A.
            and Wijmenga,C.
  TITLE     Coeliac disease-associated risk variants in TNFAIP3 and REL
            implicate altered NF-kappaB signalling
  JOURNAL   Gut 58 (8), 1078-1083 (2009)
   PUBMED   19240061
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   10 (bases 1 to 9367)
  AUTHORS   Cox,G.A., Mahaffey,C.L., Nystuen,A., Letts,V.A. and Frankel,W.N.
  TITLE     The mouse fidgetin gene defines a new role for AAA family proteins
            in mammalian development
  JOURNAL   Nat Genet 26 (2), 198-202 (2000)
   PUBMED   11017077
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC092684.2 and AC093727.3.
            
            On May 31, 2019 this sequence version replaced NM_001321825.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.165059.1,
                                           SRR7346977.2878945.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142853
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-171               AC092684.2         59684-59854         c
            172-9367            AC093727.3         1513-10708          c
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q24.3"
     gene            1..9367
                     /gene="FIGN"
                     /note="fidgetin, microtubule severing factor"
                     /db_xref="GeneID:55137"
                     /db_xref="HGNC:HGNC:13285"
                     /db_xref="MIM:605295"
     exon            1..171
                     /gene="FIGN"
                     /inference="alignment:Splign:2.1.0"
     exon            172..9367
                     /gene="FIGN"
                     /inference="alignment:Splign:2.1.0"
     CDS             180..2426
                     /gene="FIGN"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /codon_start=1
                     /product="fidgetin isoform 2"
                     /protein_id="NP_001308754.1"
                     /db_xref="GeneID:55137"
                     /db_xref="HGNC:HGNC:13285"
                     /db_xref="MIM:605295"
                     /translation="MQWTPEHAQWPEQHFDITSTTRSPAHKVEAYRGHLQRTYQYAWA
                     NDDISALTASNLLKKYAEKYSGILEGPVDRPVLSNYSDTPSGLVNGRKNESEPWQPSL
                     NSEAVYPMNCVPDVITASKAGVSSALPPADVSASIGSSPGVASNLTEPSYSSSTCGSH
                     TVPSLHAGLPSQEYAPGYNGSYLHSTYSSQPAPALPSPHPSPLHSSGLLQPPPPPPPP
                     PALVPGYNGTSNLSSYSYPSASYPPQTAVGSGYSPGGAPPPPSAYLPSGIPAPTPLPP
                     TTVPGYTYQGHGLTPIAPSALTNSSASSLKRKAFYMAGQGDMDSSYGNYSYGQQRSTQ
                     SPMYRMPDNSISNTNRGNGFDRSAETSSLAFKPTKQLMSSEQQRKFSSQSSRALTPPS
                     YSTAKNSLGSRSSESFGKYTSPVMSEHGDEHRQLLSHPMQGPGLRAATSSNHSVDEQL
                     KNTDTHLIDLVTNEIITQGPPVDWNDIAGLDLVKAVIKEEVLWPVLRSDAFSGLTALP
                     RSILLFGPRGTGKTLLGRCIASQLGATFFKIAGSGLVAKWLGEAEKIIHASFLVARCR
                     QPSVIFVSDIDMLLSSQVNEEHSPVSRMRTEFLMQLDTVLTSAEDQIVVICATSKPEE
                     IDESLRRYFMKRLLIPLPDSTARHQIIVQLLSQHNYCLNDKEFALLVQRTEGFSGLDV
                     AHLCQEAVVGPLHAMPATDLSAIMPSQLRPVTYQDFENAFCKIQPSISQKELDMYVEW
                     NKMFGCSQ"
     regulatory      4342..4347
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FIGN"
                     /note="hexamer: AATAAA"
     polyA_site      4365
                     /gene="FIGN"
     regulatory      9340..9345
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FIGN"
                     /note="hexamer: AATAAA"
     polyA_site      9367
                     /gene="FIGN"
                     /note="major polyA site"
ORIGIN      
        1 gttttgggtt tgaaattcca acatggcaga ggctgcagtc cgtcttccct tcaaaaactt
       61 ggaatgattt caaatcatag gcaccttcac ttaaccctag cttccattca tcagcaaaca
      121 catcggatcg atgctacgct aacctatcgg gttctctctc cgcgcgttca ggcttgaaga
      181 tgcagtggac gccagagcat gcccagtggc cagaacagca ctttgacatc acctcaacca
      241 ctcggtctcc tgcccacaaa gttgaagcct acagaggtca tctgcagcgc acctatcagt
      301 acgcctgggc gaatgatgac atatctgctc tgactgcatc caacctacta aaaaaatatg
      361 cagagaagta ttccggcatt ttggaaggtc ctgtggaccg acccgtactc agcaactatt
      421 cggacacacc atcaggacta gtgaacggtc ggaaaaatga aagtgaaccc tggcagcctt
      481 ccttgaattc agaagctgtt tatcccatga actgtgttcc ggatgttatc actgccagca
      541 aagctggagt cagttcagcc ctccctccag cagatgtctc tgcgagtata ggaagctctc
      601 ctggggtagc cagcaacctg acagaaccta gttattcaag tagtacctgt ggaagccaca
      661 ctgtacccag tcttcatgca gggctcccat ctcaggaata tgccccagga tacaacggat
      721 catatttgca ttctacttat agtagccagc cagcacctgc acttccttca cctcatccgt
      781 ctcctttgca tagctctggg ctactacagc ccccaccacc acctcctccg ccaccagcct
      841 tggtcccagg ctacaatggg acttctaacc tctccagtta cagctatccg tctgctagct
      901 atcctcctca gactgctgtg gggtctgggt acagccctgg gggggcaccg cctccgcctt
      961 cagcgtacct gccttcagga attcctgctc ccacccccct accccccacc actgttcctg
     1021 gctacaccta ccagggccat ggtttgacac ctattgcacc gtcggctctg acaaacagtt
     1081 cagcaagttc tctcaaaagg aaagctttct acatggcagg gcaaggagat atggactcca
     1141 gttatggaaa ttacagctat ggccaacaga gatctacaca gagtcctatg tacagaatgc
     1201 ccgacaacag catttcaaac acaaatcggg ggaatggctt tgacagaagt gctgaaacat
     1261 catccttagc atttaagcca acgaagcagc taatgtcctc tgaacagcaa aggaaattca
     1321 gcagccagtc cagtagggct ctgacccctc cttcctacag tactgctaaa aattcattgg
     1381 gatcaagatc cagtgaatcc tttgggaagt acacatcgcc agtaatgagt gagcatgggg
     1441 acgagcacag gcagctcctc tctcacccaa tgcaaggccc tggactccgt gcagctacct
     1501 catccaacca ctctgtggac gagcaactga agaatactga cacgcacctc atcgacctgg
     1561 taaccaatga gattatcacc caaggacctc cagtggactg gaatgacatt gctggtctcg
     1621 acctggtgaa ggctgtcatt aaagaggagg ttttatggcc agtgttgagg tcagacgcgt
     1681 tcagtggact gacggcctta cctcggagca tccttttatt tggacctcgg gggacaggca
     1741 aaacattatt gggcagatgc atcgctagtc agctgggggc cacatttttc aaaattgccg
     1801 gttctggact agtcgccaag tggttaggag aagcagagaa aattatccat gcctcttttc
     1861 ttgtggccag gtgtcgccag ccctcggtga tttttgttag tgacattgac atgcttctct
     1921 cctctcaagt gaatgaggaa catagtccag tcagtcggat gagaaccgaa tttctgatgc
     1981 aactggacac tgtactaact tcggctgagg accaaatcgt agtaatttgt gccaccagta
     2041 aaccagaaga aatagatgaa tcccttcgga ggtacttcat gaaacgactt ttaatcccac
     2101 ttcctgacag cacagcgagg caccagataa tagtacaact gctctcacag cacaattact
     2161 gtctcaatga caaggagttt gcactgctcg tccagcgcac agaaggcttt tctggactag
     2221 atgtggctca tttgtgtcag gaagcagtgg tgggccccct ccatgccatg ccagccacag
     2281 acctttcagc cattatgccc agccagttga ggcccgttac atatcaagac tttgaaaatg
     2341 ctttctgcaa gattcagcct agcatatctc aaaaggagct tgatatgtat gttgaatgga
     2401 acaaaatgtt tggttgcagt cagtgataac ttctttagaa aaaaaaaatg taatgaatgt
     2461 tggcacacac acataaaacc tgctacatag ggaatagagc ccctttccag tagagtttaa
     2521 attgcaaagg gtactgggga agatgacgat taagttgcat ctttagagtc agggtagatt
     2581 tggaggaaaa gtgcatcaaa tgagagcttc tgatttgaaa gccccagatg acagaaagca
     2641 tatgtggatg ctcagttctg ttcaagctag acaacactca ccaaggagca aggtgcaagt
     2701 gtgttgattt cagaaggaca tgaacctcgt gtgttgattc cattctgctg ttctcgagat
     2761 ttagttgctg tcaagtgcct ggagtggtgc tttatttttt gtttgcctca caattacatt
     2821 ggtggcatgt gctaatataa agagctttaa cttcaaacat tattggacta aagagatgaa
     2881 cagttgtgtt atgacagaaa accagatttt tgccatttta agagcaacag tattcctcaa
     2941 tcctgtctgt tctgcagtat taagctaaga acaggtaaaa cagggtaacg gtaatctgga
     3001 ccttaatttc tgcagttcat ttcttttaat gttcttgtct gcaaaaactc aggaaagtga
     3061 ttgtgatttg tacagtacct caaaggaatg tgttgaaagc actatgtact gctgagagta
     3121 ataggatagg cttcaatgtt actttatatt aaaatgtatg tttacctcaa caattggaaa
     3181 atagcaagga aaattacttt gaatgtatcc agaaaaatac tgaagtgtga tacaactgaa
     3241 tatttacagt ttaaagtaga aatggaagga tttttttaag ttcttttact aattatgggg
     3301 aattaaccag agcagaataa ttctttatgt caataactgc aagagttctt agtacattgc
     3361 tccttgataa ttaagtgaaa atgttcttaa aaggtacact ggttaattga aagctactta
     3421 ttcagtttgt gttagtgtct agacctgtca gccacaagac ctgtttagga ccctgaaagt
     3481 cacagtacct aaaaactatg actgcctttt tattgcatag gtggtagtgg tggtgatggt
     3541 ggtggtagtt tgcaagttat ctcttaaaac tgctgggaat ggtgtcattc tattcactaa
     3601 tctagcttat agacttgccg tgctgtttga tagaatgcag aggatagcaa ccaaaacaaa
     3661 tacacaaata aataaaaaca aaaaccaacc aacaaaccaa cttacataca catatatata
     3721 tccacaaaga acctctccat ctcctcccct tctttttgac tccactcttg tcagtgcaat
     3781 tttgcttctc attttgaaat ctgggctgta gtgctcctgc tttatttcta cctcagtttt
     3841 gttacatttc tcttggaaag taaagtagaa aattggaagt ggacacacac actgcaatgt
     3901 agcttgccaa acatgttact ttgttttctt ccatctttca ccgtaaatct agtttccaaa
     3961 gacatcagca tttgtgctta cttccacctc agtctaccag ccccacccct acccatggca
     4021 taagtggcat ttttcttaat ttcctatttt tctcctgctc tctgtcaagt tgttctttgt
     4081 atcctttaat gctttatgtg caacctttca ttgatagtgg gctgatgttt ggcaatgctt
     4141 ctgaactgtc acagagcagg ctgtagcttt ccacagccac tgcccatgca taagcagaac
     4201 agcctggcct tttgaatgta ttttcctggg ttttttcccc ttttcttttt ttagtttaga
     4261 gatgcagtaa caaaactgtt gcaaagcact ggcattttat gtattcaata aataagtgat
     4321 gtacattttt aaaaaaattt aaataaatgc aatgagaagc cccaagaaag gcctttctgt
     4381 tttttttttc ttacaaaaat ttttaccgat cttatccaca atatccaatt ctctgaaaca
     4441 cttaacatag agaaagctca tgagggattt cttatctctg aaattttatg atgcttgatc
     4501 tttactcccc tttttttatt ttttatgttg ctaaaaactg agcaattgaa ccagaattta
     4561 atttactgtg acattctgtc aattaaagct gtgcttaaat ataaatattc catattaaaa
     4621 agcagtgcct ttcattaagg atacaaaaaa taatacttaa atacgccctc tgcttttcgc
     4681 aagtgaataa atgctacaat cgtgttctgg gtctaccctg tgcatccctt gtaaggaaat
     4741 tctaatatcc acattaactt gtaggttcat attgatctca ctgacaagtt tacttttgca
     4801 cagtgctgac ctattttctg acttttagac attatatttc atttccgcca acatgctgtg
     4861 ttcacttgaa agcgtctggc aagttttgac aaacccagtc ttatttagac aactttggaa
     4921 aatccaatat tgacccacat ttttttgtgg ctagtaagat ttaatgccaa atatatgact
     4981 gcactattgt gttttacact catttataaa actgaaaata aattgaagtc atacatcttg
     5041 gcttttagct ttaagtgact taaaaattag aactctgagt gacttaaaaa ttaatttgag
     5101 atgcaagtgg tcaaaaatcc atgtatttag acagaagctg aagtcgtatg tgattttcat
     5161 ttagcctcac aaaatccaag atttgatctc ttaggaaatt gggcatgttg aagaaatgaa
     5221 gatctgttgg aggagatatg gctgaccttt gagtagatag tgctctcctg tgattctttg
     5281 attctgcatg gcagcattat gagctcttct ataaactagc ttaataatga gctctattct
     5341 gaccatctat tctggaactt ggagtggggg tggggtacca catcctcaga agtgaatgtg
     5401 tacagtcggt gatagtacaa ctgagtaatg gggcttcata cccttggcct ctttttgact
     5461 gctaagaatt tggagaaggc tattaatgaa ggtaaggtaa tgagccagtc caaatccctc
     5521 actgtctggc taagagattg agccattcca ctttacagcc cctaattccc ttcttcccaa
     5581 ctgcagcaac atttccaaca gggacaaaaa ggaagtgaag gaaaccactt cagtattggg
     5641 atcaatagaa attcaagagg tagttcataa tgaccttata tgtgacacaa atggaaatac
     5701 tctacacttc tggcatgctt ctctcccaaa tatttagaac attagtgaca atatgaaaaa
     5761 ctttgctgac tgagctgatc aatggaatgt gaaggcagta tactttaagg ctatttactt
     5821 tcacacagaa tgtttatcta ttagtcagtt tgtattctac tcaaatgcag atagacgggt
     5881 ataatgtttt cttgtgcatt tgaaacctat caaggagaaa ataaaccatt ccactaaaat
     5941 acataggcca aacaccatct gggaaagtga taaaagctat ccagaggcta tgctatgtaa
     6001 aatagcaagg tcaatttaca tgggttgcat agtacttggc tatgcagttt tcaaaagaac
     6061 acatccaaaa aaaaaaaaaa tccaaattag ttcctataat aggtctttgt aaatttaaat
     6121 atatttgtgg gttggaaaat acgtttttag agacatttag aatttctttc tctaaaaatg
     6181 tatccaactc caagcagaaa gagacttctg tactcacatt ctcatgtttc ttagaattgg
     6241 gtagcatgat cattgaatct gtattttgca aagtgaaagg gacgcagctt gttgaactat
     6301 tgaggccact aaagtaaagt tctaatatat atatataaat ataaaattag tgtgtgtgtt
     6361 tgtatgtgtg tatgtgtgtg tgttctttca tcaccaccta gttgaacttt aaaacagacg
     6421 tctacttttc attattcaga agcaacttgt tacttccttt cttcatatgt gcagagatac
     6481 acatgatcat gttacaattt gaagtgtttt aatgtgtttt tttttctttt gaaacgtaaa
     6541 gccttcagtt gttgaatcat ctgccgattt ttatatgaag cacattagca aacctggagc
     6601 tattagtatt tcagaattca caaccagctg tacttgtgac taaccaacac atgaactgaa
     6661 gctcacactt tgctaagacc tttggttgac attcgatttt gaacctgcca gatgcatttg
     6721 tgtgaaccag ggatactgca tctttaatga acaagtctct cctctttccc ctgtttttaa
     6781 atacaacaca agcagtgtag cactaggcac tgcaattgtc aaagacaatt agactggaag
     6841 caggaaaatt tcacctccct gtctcagtga tatgtagaat gtcggcagca tgggtcagcc
     6901 ttgccacaga gttgtgtgag aatatatgaa tcagtcctat agaatgttaa atatttataa
     6961 aatggttctg tagtacacca aggttgggag cacattcatt ccttcttgtt gtcttatgat
     7021 acaaaaaaaa aaaaaaaaaa agcaaaagtt tatttctggg cttaaaactc tccctctaat
     7081 atctctctct cttttacccc cattccccaa ccattcttct ctccatccct ctcctctcct
     7141 cttcctctct cttctctctc tctctctctc tctctgtctc taactctcgc tctctcttgt
     7201 atgtgggtgt gtgtgtgtat gagtttccac tgtggattat atacacttgc tagagaatat
     7261 tcatttcttt tttttctttt ttcatctgat gtatattcaa acattgttct ttataaccat
     7321 ttctacctca ttgctacata ttgtacatgt agtatttatt tgttgtcttt ttttgaatgt
     7381 catgcatttc gtaagaaaaa gacttgtcct tgaacttgaa cagtctacct cagaaagact
     7441 gtctttacac tgaacagtat tagcaaccta aatgataaga cgcattagtg aagtgtggca
     7501 gggtagataa tgcacgagta cttgggatac tgatggactt ttaattgtat tcataacaca
     7561 agactgctta aaagaactta aaagaaatta tgcactgtgt aggtcttcat cgaggtccca
     7621 tttgggacat tatatacaga atagagcttt attcctatgt gcccatctct gagaaatgtg
     7681 ttagcattcc caagtatttt acactggcaa tctggctact taagacaaac aaattcaaac
     7741 aacattttta tattttgact gacaccctca gctcctaaat ttaagagaaa taatggtact
     7801 taactatttt caactatagt cttcagtgat tgaaaatgcc ttcattttaa ttctgtgact
     7861 cctgatataa attaagaaaa acaatgcagt tgcaagggcc tgcacaacac tgaaaataaa
     7921 ccaaaaatta aaaatggcac ttcccaaagg aattaacaaa cgtggagtca tggtgcttgc
     7981 aagttccata actgaaagca ttttgtttct gatttccctt tataacatgt atttgtttat
     8041 tgaagcaatt gtacaactaa tggcacattg aattactttg tatgaagtgt tttatattat
     8101 actatttatt ttagcattcg ttctttttct tcaccagctc tatattacag tagcaaaaga
     8161 acacttagtt ttaaaaaatg ctgaaagggg ttcaccaccc acccaatcga tgaatggact
     8221 tttgaagttg cttgtcagca aattgtggat attttacaaa agcaaatgaa ctgtgacaat
     8281 ccacaactcc cacagatgtg aattcagaaa tgcatctgaa actccggtat gcctgacaat
     8341 ctattcttta agtttcatta acaaagtggc atccaatcct ttcacttaca attccttgca
     8401 ggaatgtatt gtctagaggc tgtctagtag tgattaagag agggtttttg ttttgtttta
     8461 tttgtttact gtattaggtg aggaatgcca tatttcttgt cagccaacag tacttgtctg
     8521 agacagaaat ttaaaaaaag aaaaagaaac ctaactaatt aaaatttctg tgcactttgc
     8581 ttccctgacc atattgattt tatttgctta aattgaaaca atacgctgga tttgaccaga
     8641 ttttgacccc ttgaaagaag aaaagcccta tatagtacat gtttctttgg gttattctag
     8701 tactgtttcc ttatttgttc tgcaaatttc aaatcatttt tttgtgtgca ttattttaca
     8761 tctgctgaaa ctaaatgtgt tattattgca ctcattaaaa atgaaacaat ccatttctct
     8821 tggaaaaaaa tgacaagctt tagatgatga aagcttaaat tatccatcac ttgcaaaatg
     8881 aattcatata atttgtgaac attattaatg taaatgagac atttctgctt agattttttt
     8941 tattttgatg ggatcattat atggttgatc atatgtgtgt aggaagctgc tgtacaatta
     9001 acttggagat ctgaaaatgc tttttgtcct cattgttgca gtttcaattg taatccattg
     9061 catctcattt tcttgggtgt tggcactgta atatattaaa aaaaaattca gtgctcagta
     9121 ttgtaacaac tgtccctact gaatattcct tttcataaat acttgctacc acagggaaat
     9181 taagttttca tatagaataa ctagtttcag tagtaaccat tgaagaatta aaaatgtggt
     9241 tcaaggcaga tatttttatg aaaagttttc tctattgtaa aatttgttgt atatggctat
     9301 gctactatca tggaataaga aaagaacaag catacctcaa ataaaaaaaa attctgagtt
     9361 aaaaaaa
//
