LOCUS       NM_001281740            5682 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens formin homology 2 domain containing 3 (FHOD3),
            transcript variant 3, mRNA.
ACCESSION   NM_001281740 XM_005258345
VERSION     NM_001281740.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Piqueras-Flores,J., Villacorta-Arguelles,E., Galvin,J.,
            Climent-Paya,V., Escobar-Lopez,L.E., Amor-Salamanca,A.,
            Garcia-Hernandez,S., Esmonde,S., Martinez-Del Rio,J.,
            Soto-Perez,M., Garcia-Pavia,P. and Ochoa,J.P.
  TITLE     Intermediate-effect size p.Arg637Gln in FHOD3 increases risk of HCM
            and is associated with an aggressive phenotype in homozygous
            carriers
  JOURNAL   J Med Genet 61 (5), 423-427 (2024)
   PUBMED   38160043
  REMARK    GeneRIF: Intermediate-effect size p.Arg637Gln in FHOD3 increases
            risk of HCM and is associated with an aggressive phenotype in
            homozygous carriers.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Vodnjov,N., Toplisek,J., Maver,A., Cuturilo,G., Jaklic,H.,
            Teran,N., Visnjar,T., Skrjanec Pusenjak,M., Hodzic,A.,
            Miljanovic,O., Peterlin,B. and Writzl,K.
  TITLE     A novel splice-site FHOD3 founder variant is a common cause of
            hypertrophic cardiomyopathy in the population of the Balkans-A
            cohort study
  JOURNAL   PLoS One 18 (12), e0294969 (2023)
   PUBMED   38051749
  REMARK    GeneRIF: A novel splice-site FHOD3 founder variant is a common
            cause of hypertrophic cardiomyopathy in the population of the
            Balkans-A cohort study.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5682)
  AUTHORS   Wu,G., Ruan,J., Liu,J., Zhang,C., Kang,L., Wang,J., Zou,Y. and
            Song,L.
  TITLE     Variant Spectrum of Formin Homology 2 Domain-Containing 3 Gene in
            Chinese Patients With Hypertrophic Cardiomyopathy
  JOURNAL   J Am Heart Assoc 10 (5), e018236 (2021)
   PUBMED   33586461
  REMARK    GeneRIF: Variant Spectrum of Formin Homology 2 Domain-Containing 3
            Gene in Chinese Patients With Hypertrophic Cardiomyopathy.
REFERENCE   4  (bases 1 to 5682)
  AUTHORS   Iskratsch,T., Reijntjes,S., Dwyer,J., Toselli,P., Degano,I.R.,
            Dominguez,I. and Ehler,E.
  TITLE     Two distinct phosphorylation events govern the function of muscle
            FHOD3
  JOURNAL   Cell Mol Life Sci 70 (5), 893-908 (2013)
   PUBMED   23052206
  REMARK    GeneRIF: C-terminal phosphorylation by ROCK1 is sufficient for
            FHOD3 activation as evidenced by an increase in F-actin in HeLa
            cells.
REFERENCE   5  (bases 1 to 5682)
  AUTHORS   Arimura,T., Takeya,R., Ishikawa,T., Yamano,T., Matsuo,A.,
            Tatsumi,T., Nomura,T., Sumimoto,H. and Kimura,A.
  TITLE     Dilated cardiomyopathy-associated FHOD3 variant impairs the ability
            to induce activation of transcription factor serum response factor
  JOURNAL   Circ J 77 (12), 2990-2996 (2013)
   PUBMED   24088304
  REMARK    GeneRIF: DCM-associated FHOD3 variant may cause DCM by interfering
            with actin filament assembly.
REFERENCE   6  (bases 1 to 5682)
  AUTHORS   Iskratsch,T., Lange,S., Dwyer,J., Kho,A.L., dos Remedios,C. and
            Ehler,E.
  TITLE     Formin follows function: a muscle-specific isoform of FHOD3 is
            regulated by CK2 phosphorylation and promotes myofibril maintenance
  JOURNAL   J Cell Biol 191 (6), 1159-1172 (2010)
   PUBMED   21149568
REFERENCE   7  (bases 1 to 5682)
  AUTHORS   Taniguchi,K., Takeya,R., Suetsugu,S., Kan-O,M., Narusawa,M.,
            Shiose,A., Tominaga,R. and Sumimoto,H.
  TITLE     Mammalian formin fhod3 regulates actin assembly and sarcomere
            organization in striated muscles
  JOURNAL   J Biol Chem 284 (43), 29873-29881 (2009)
   PUBMED   19706596
  REMARK    GeneRIF: actin dynamics regulated by Fhod3 are critical for
            sarcomere organization in striated muscle cells.
REFERENCE   8  (bases 1 to 5682)
  AUTHORS   French,D., Yang,W., Cheng,C., Raimondi,S.C., Mullighan,C.G.,
            Downing,J.R., Evans,W.E., Pui,C.H. and Relling,M.V.
  TITLE     Acquired variation outweighs inherited variation in whole genome
            analysis of methotrexate polyglutamate accumulation in leukemia
  JOURNAL   Blood 113 (19), 4512-4520 (2009)
   PUBMED   19066393
  REMARK    GeneRIF: Single nucleotide polymorphism in FHOD3 gene is associated
            with acute lymphoblastic leukemia.
REFERENCE   9  (bases 1 to 5682)
  AUTHORS   Kanaya,H., Takeya,R., Takeuchi,K., Watanabe,N., Jing,N. and
            Sumimoto,H.
  TITLE     Fhos2, a novel formin-related actin-organizing protein, probably
            associates with the nestin intermediate filament
  JOURNAL   Genes Cells 10 (7), 665-678 (2005)
   PUBMED   15966898
REFERENCE   10 (bases 1 to 5682)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     Identification and characterization of human FHOD3 gene in silico
  JOURNAL   Int J Mol Med 13 (4), 615-620 (2004)
   PUBMED   15010865
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY020952.1, AK128053.1,
            HM191478.1, AC131053.2, BC031518.1, BC081563.1, AI948493.1 and
            AC090333.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001281740.2.
            
            Summary: The protein encoded by this gene is a member of the
            diaphanous-related formins (DRF), and contains multiple domains,
            including GBD (GTPase-binding domain), DID (diaphanous inhibitory
            domain), FH1 (formin homology 1), FH2 (formin homology 2), and DAD
            (diaphanous auto-regulatory domain) domains. This protein is
            thought to play a role in actin filament polymerization in
            cardiomyocytes. Mutations in this gene have been associated with
            dilated cardiomyopathy (DCM), characterized by dilation of the
            ventricular chamber, leading to impairment of systolic pump
            function and subsequent heart failure. Increased levels of the
            protein encoded by this gene have been observed in individuals with
            hypertrophic cardiomyopathy (HCM). Alternative splicing results in
            multiple transcript variants encoding different isoforms. A
            muscle-specific isoform has been shown to possess a casein kinase 2
            (CK2) phosphorylation site at the C-terminal end of the FH2 domain.
            Phosphorylation of this site alters its interaction with
            sequestosome 1 (SQSTM1), and targets this isoform to myofibrils,
            while other isoforms form cytoplasmic aggregates. [provided by
            RefSeq, Aug 2015].
            
            Transcript Variant: This variant (3) contains additional in-frame
            coding exons compared to variant 1. The resulting longer isoform
            (3) is muscle-specific and required for the maintenance of
            myofibrils in heart muscle (PMID:21149568).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: HM191478.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2151741, SAMEA2154361
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000590592.6/ ENSP00000466937.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-108               HY020952.1         18-125
            109-727             AK128053.1         105-723
            728-1529            HM191478.1         605-1406
            1530-1530           AC131053.2         8579-8579           c
            1531-1942           HM191478.1         1408-1819
            1943-3300           BC031518.1         887-2244
            3301-4992           HM191478.1         3178-4869
            4993-5521           BC081563.1         1588-2116
            5522-5673           AI948493.1         17-168              c
            5674-5674           AC090333.5         31049-31049         c
            5675-5682           AI948493.1         8-15                c
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q12.2"
     gene            1..5682
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /note="formin homology 2 domain containing 3"
                     /db_xref="GeneID:80206"
                     /db_xref="HGNC:HGNC:26178"
                     /db_xref="MIM:609691"
     exon            1..288
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..4992
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /note="isoform 3 is encoded by transcript variant 3;
                     FH1/FH2 domain-containing protein 3; formactin-2; formin
                     homolog overexpressed in spleen 2"
                     /codon_start=1
                     /product="FH1/FH2 domain-containing protein 3 isoform 3"
                     /protein_id="NP_001268669.1"
                     /db_xref="CCDS:CCDS62418.1"
                     /db_xref="GeneID:80206"
                     /db_xref="HGNC:HGNC:26178"
                     /db_xref="MIM:609691"
                     /translation="MATLACRVQFLDDTDPFNSTNFPEPSRPPLFTFREDLALGTQLA
                     GVHRLLQAPHKLDDCTLQLSHNGAYLDLEATLAEQRDELEGFQDDAGRGKKHSIILRT
                     QLSVRVHACIEKLYNSSGRDLRRALFSLKQIFQDDKDLVHEFVVAEGLTCLIKVGAEA
                     DQNYQNYILRALGQIMLYVDGMNGVINRNETIQWLYTLIGSKFRLVVKTALKLLLVFV
                     EYSESNAPLLIQAVTAVDTKRGVKPWSNIMEILEEKDGVDTELLVYAMTLVNKTLSGL
                     PDQDTFYDVVDCLEELGIAAVSQRHLNKKGTDLDLVEQLNIYEVALRHEDGDETTEPP
                     PSGCRDRRRASVCSSGGGEHRGLDRRRSRRHSVQSIKSTLSAPTSPCSQSAPSFKPNQ
                     VRDLREKEEEEEEEQPITEPSSEEEREDDASCQGKDSKVGAASGQSPTGRDAAPKSSA
                     LPAVSNASSQGKPLLVGTAGGTTWHSGSSGSEATPSALLSPPASAARPSSATPGSLKV
                     SPTIDKLPYVPHSPFHLFSYDFEDSSLSTKEKEAESQKENSSSDSFSLSTYSASEPYH
                     FRSFSSNRYSNFGNNSYHSSRPSSGSSVPTTPTSSVSPPQEARLERSSPSGLLTSSFR
                     QHQESLAAERERRRQEREERLQRIEREERNKFSRDYLDKREEQRQAREERYKYLEQLA
                     AEEHEKELRSRSVSRGRADLSLDLTSPAAPACLAPLSHSPSSSDSQEALTVSASSPGT
                     PHHPQASAGDPEPESEAEPEAEAGAGQVADEAGQDIASAHEGAETEVEQALEQEPEER
                     ASLSEKERQNEGVNERDNCSASSVSSSSSTLEREEKEDKLSRDRTTGLWPAGVQDAGV
                     NGQCGDILTNKRFMLDMLYAHNRKSPDDEEKGDGEAGRTQQEAEAVASLATRISTLQA
                     NSQTQDESVRRVDVGCLDNRGSVKAFAEKFNSGDLGRGSISPDAEPNDKVPETAPVQP
                     KTESDYIWDQLMANPRELRIQDMDFTDLGEEDDIDVLDVDLGHREAPGPPPPPPPTFL
                     GLPPPPPPPLLDSIPPPPVPGNLLVPPPPVFNAPQGLGWSQVPRGQPTFTKKKKTIRL
                     FWNEVRPFDWPCKNNRRCREFLWSKLEPIKVDTSRLEHLFESKSKELSVSKKTAADGK
                     RQEIIVLDSKRSNAINIGLTVLPPPRTIKIAILNFDEYALNKEGIEKILTMIPTDEEK
                     QKIQEAQLANPEIPLGSAEQFLLTLSSISELSARLHLWAFKMDYETTEKEVAEPLLDL
                     KEGIDQLENNKTLGFILSTLLAIGNFLNGTNAKAFELSYLEKVPEVKDTVHKQSLLHH
                     VCTMVVENFPDSSDLYSEIGAITRSAKVDFDQLQDNLCQMERRCKASWDHLKAIAKHE
                     MKPVLKQRMSEFLKDCAERIIILKIVHRRIINRFHSFLLFMGHPPYAIREVNINKFCR
                     IISEFALEYRTTRERVLQQKQKRANHRERNKTRGKMITDTDEEEEVESGKFSGSSPAP
                     PSQPQGLSYAEDAAEHENMKAVLKTSSPSVEDATPALGVRTRSRASRGSTSSWTMGTD
                     DSPNVTDDAADEIMDRIVKSATQVPSQRVVPRERKRSRANRKSLRRTLKSGLTPEEAR
                     ALGLVGTSELQL"
     exon            289..395
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            396..460
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            461..528
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            529..634
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            635..729
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            730..841
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            842..936
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1080
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1319
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1409
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1769
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            1770..1844
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..1958
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            1959..2093
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            2094..2144
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            2145..2359
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            2360..2656
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            2657..3540
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            3541..3699
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            3700..3882
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            3883..4002
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            4003..4164
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            4165..4355
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            4356..4548
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            4549..4572
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            4573..4747
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            4748..4909
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     exon            4910..5682
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5498..5503
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /note="hexamer: AATAAA"
     regulatory      5502..5507
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /note="hexamer: AATAGA"
     polyA_site      5518
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
                     /note="major polyA site"
     polyA_site      5521
                     /gene="FHOD3"
                     /gene_synonym="CMH28; FHOS2; Formactin2"
ORIGIN      
        1 gagcctgcga gtccgcgagc cagcgagctg cggctgcggc ctcccctgcg cgcagctacc
       61 cgggcgtccc ggcccgcggc cccgctaacc ccggggcccg cgcccccgcg gcagggatgc
      121 atcatggcca cgctggcttg ccgggtgcag ttcttggacg acacggaccc tttcaacagc
      181 accaacttcc ccgagcccag ccggccgccg ctgttcacgt tccgcgagga cctcgcgctc
      241 ggcacccagc tggcgggggt ccataggctg ctgcaggcgc cgcacaagct ggatgactgt
      301 actctgcagc tctctcacaa tggcgcctac ctggatttgg aggccaccct ggcagagcag
      361 cgggatgagt tggaaggctt ccaggatgac gccgggcggg gcaagaagca cagcatcatc
      421 ctaaggacgc agctgtctgt gagggtccat gcctgcatcg aaaaactata caactccagc
      481 ggacgagatt tgagaagggc cctcttctcc ctgaagcaga tatttcagga tgacaaggat
      541 ttggtgcatg aatttgtagt ggctgaaggt ctgacatgtt tgatcaaggt gggagctgag
      601 gctgatcaga actatcagaa ctacatctta agggctttgg gccagattat gttgtatgtg
      661 gatggaatga atggagtaat aaaccgcaat gaaaccattc agtggctgta cactctcatt
      721 gggtcaaagt tccgcctggt ggtgaagaca gccctgaagc tgctgctcgt ctttgtagag
      781 tactcggagt ccaacgcacc tctcctaatt caggctgtca ctgctgttga cacgaaaaga
      841 ggggtcaaac cttggtcaaa tatcatggaa atcctggagg aaaaagatgg agttgatacg
      901 gagctactgg tttatgcaat gactttggtg aacaagacgt tatcaggact accagaccaa
      961 gacaccttct acgacgtcgt ggactgcctg gaggagctgg gcattgctgc tgtgtcccag
     1021 aggcacttga acaagaaagg gactgacctg gacttagtgg agcaactcaa catttatgag
     1081 gtggcgctca ggcacgagga tggcgatgag accacggagc caccccccag tgggtgccgg
     1141 gaccggagga gggccagcgt gtgttccagt ggcggaggcg agcaccgggg cctggaccgc
     1201 agaaggagcc gcaggcactc ggtgcagagc atcaagagca ccctgtcggc ccccaccagt
     1261 ccctgctccc agtcagctcc cagcttcaag cccaaccaag tgcgagatct gcgtgaaaag
     1321 gaggaggagg aggaagagga gcagccaatc acggagccca gttccgaaga agagagagag
     1381 gatgatgctt cctgtcaggg caaggacagc aaggtcggcg ctgcctcagg gcagagcccc
     1441 actggaaggg atgctgctcc caagagctct gccctccctg ctgtctcgaa tgccagctcg
     1501 cagggaaagc cgcttctggt tggcactgca ggcgggacca cctggcacag tgggtcctct
     1561 gggtctgagg ccaccccatc tgccctcctg tcaccccctg cctcagctgc tcggccctcc
     1621 tccgccacac caggctccct gaaggtgtca ccgaccatag acaagctgcc ctacgtgccc
     1681 cacagcccct tccacctctt ctcctatgac tttgaggact cctccctgtc caccaaggag
     1741 aaggaagcag agtcccagaa ggaaaacagt tcctctgatt ctttctcttt gagcacatat
     1801 tctgcctctg agccttacca cttccgatct ttctcttcta atagatacag caattttggc
     1861 aataactctt atcactcctc aagaccctca tctggatcca gtgtgcccac cacccccaca
     1921 tcatccgtct cacccccaca ggaggccagg ttggaaaggt catcaccgag tggtcttctc
     1981 acatcatcct tcaggcagca ccaagagtca ctggcagcag agagagagag gcggcggcag
     2041 gagagagaag aaaggttgca gagaatagag cgggaagaaa gaaacaaatt cagccgagat
     2101 tatttagaca aaagagagga gcaaaggcaa gcaagagaag aaagatacaa atacttggaa
     2161 cagttggcag ctgaggagca cgagaaggag ctgagaagcc ggagtgtgag ccggggcaga
     2221 gccgacctct ccttggacct gacctcgcca gcagccccag cctgcctggc tcctctgagc
     2281 catagcccct catcttcaga ctctcaagag gctctcacgg tgtctgcctc ctccccagga
     2341 acccctcacc atccccaagc aagtgccggg gatcctgaac ccgaatcaga ggcagaaccg
     2401 gaagcagagg caggggcggg gcaggttgct gatgaagctg gccaggacat agcctctgcc
     2461 cacgagggtg cagagactga agtggagcag gcactagagc aagagccgga agaaagagcc
     2521 tccctcagtg aaaaagagag gcagaacgag ggggtgaacg agagggacaa ctgctctgcc
     2581 tccagcgtct cgtcctccag cagcacgttg gagagggagg agaaggagga caagctctcc
     2641 agggacagga caactggttt gtggcccgca ggtgtccagg atgcaggtgt aaatggacag
     2701 tgtggcgaca tcctcaccaa caaacggttc atgcttgaca tgctgtatgc ccataacagg
     2761 aagtctccgg atgatgagga gaagggggat ggggaggctg ggaggaccca gcaggaggca
     2821 gaggcggtag ccagccttgc taccaggata tccaccctgc aggccaactc tcagacccag
     2881 gatgagagtg tcaggagggt ggatgtcggc tgtttggaca atcggggcag tgtgaaagca
     2941 tttgctgaga aattcaacag tggggacctg gggagaggtt ccatctcccc tgatgctgag
     3001 cccaatgaca aggtcccaga aacagcgccg gtgcagccga agacagagtc tgattacatc
     3061 tgggaccagc tcatggccaa tccaagagag ctcagaatcc aagacatgga tttcactgac
     3121 ctgggggagg aggatgacat tgatgtccta gatgtggacc tgggtcacag ggaggcccct
     3181 gggccacctc ccccaccccc acccaccttt ctgggtttgc cgcccccacc ccctccgccc
     3241 ctgttggaca gcattcctcc ccctcctgtc cctggtaatt tattggttcc tcctcctcca
     3301 gtgttcaacg ctcctcaggg cttagggtgg tcccaggtac ccaggggtca gcccacattc
     3361 actaagaaaa agaagaccat ccgtttgttc tggaatgaag ttcggccttt tgactggcca
     3421 tgtaaaaaca accgacgctg cagagaattc ctgtggtcaa aactggaacc cattaaggtg
     3481 gacacttcca gactggagca cctgtttgag tctaaatcca aggaactgtc tgtctcaaag
     3541 aaaactgctg cagatggaaa aaggcaagag atcattgttc tggattccaa gaggagtaac
     3601 gccatcaata ttggtctgac ggtgctgccc cctccaagga cgattaagat cgccattttg
     3661 aattttgatg agtatgcctt aaacaaagaa ggaatcgaga aaattctaac gatgattccc
     3721 accgatgagg agaagcagaa aatccaggaa gctcagctgg ccaaccctga aatccccctg
     3781 ggcagtgcag agcagttcct cctcaccctg tcctccatca gcgagctctc tgcacgactt
     3841 cacctctggg cattcaaaat ggattatgaa actacagaaa aggaagtagc agaaccactc
     3901 ctggacctga aggaaggaat agaccagttg gagaacaata aaaccttggg ctttatcctg
     3961 tctactctct tagccattgg gaactttcta aatggaacta atgccaaagc gtttgagtta
     4021 agctacctcg agaaggttcc agaagtcaaa gacacagtgc acaagcagtc gcttctccac
     4081 catgtgtgca ccatggtggt agaaaacttc ccagacagct ccgatctgta ctcggagatc
     4141 ggggccatca ccaggtcagc caaggttgac tttgatcaac ttcaggataa tttatgtcag
     4201 atggagagaa gatgcaaagc ttcatgggat cacctcaagg caattgcaaa acatgaaatg
     4261 aaaccagttt taaaacaacg gatgtcagag ttcctgaaag actgtgcaga gcgaattata
     4321 attttaaaga ttgtccatag aaggataatc aacagattcc actccttttt actctttatg
     4381 ggccatccac cttatgcaat tcgggaagtg aacataaaca aattctgcag gattattagt
     4441 gaatttgcac tagagtatcg cacaaccagg gaaagggttt tgcagcagaa acagaaacgg
     4501 gccaaccaca gagagagaaa taagaccaga gggaagatga tcaccgatac tgatgaggag
     4561 gaggaagttg agtctggcaa gttctccggc agttctccgg cgcccccaag ccagccgcag
     4621 ggtctgagct atgcggagga cgcggctgag cacgagaaca tgaaggctgt gctgaaaacc
     4681 tcgtccccct ccgtggagga cgccaccccc gcgctgggcg tccgcacacg cagccgagca
     4741 agccgaggat ccactagttc ctggactatg ggaactgatg actcgcccaa tgtcacagat
     4801 gatgcagctg atgagatcat ggaccgcatc gtcaagtcag ccacccaagt gcccagtcag
     4861 cgagtggtgc cgagggagag gaaacgatcc cgggccaacc ggaaatcttt gcgaagaacc
     4921 ctgaagagcg gcctgacccc agaagaagcc agagccctgg gcttggttgg cacctcggag
     4981 ttgcagctgt gacactcata ggttactccc aggagtgtgc tgagcagaag gcaagctctt
     5041 gctggatgaa acccctccag gtggggttgg ggagacttga tattcacatc caacagtttg
     5101 aaaagggaga gctcaattcc cagcgtcacc ccatggcttg tgttgcctgc tacgcattga
     5161 cttggatctc caggagtccc ctgcacatac cttctccatc gtgtcagctg tgtttctctt
     5221 gattccgtga cacccggttt attagttcaa aagtgtgaca ccttttctgg gcaaggaaca
     5281 gcccctttaa ggagcaaatc acttctgtca cagttattat ggtaatatga ggcaatctga
     5341 ttagcttcac agactgagtc tccacaacac caaaatatcc agatgtaaac cccaaacttg
     5401 tacacaaaag aaagcacaga ttgtttacct gttgtggatt ttagatgtaa caaatgttta
     5461 tacaaataca tacatgtaca ccatgtttca aatactaaat aaatagagtt taatgccata
     5521 atgagaaact tttattcttc tgggaacagg accttaaaca gttccacagg ctcgcctctt
     5581 cagaatggca aaactcttct cagtgtcctc agaagcaccc tcgcttggaa cggccttcag
     5641 atcctttggg ctgtattttg ttaatagagt gagtaacatc aa
//
