LOCUS       NM_001322466            3949 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens four and a half LIM domains 5 (FHL5), transcript
            variant 3, mRNA.
ACCESSION   NM_001322466
VERSION     NM_001322466.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Xu,X., Liang,F., Chen,J., Chen,F., Kong,L. and Ding,Y.
  TITLE     Association of FHL5 and LPA genetic polymorphisms with diabetes
            mellitus risk: a case-control study
  JOURNAL   Aging Male 26 (1), 2235005 (2023)
   PUBMED   37452735
  REMARK    GeneRIF: Association of FHL5 and LPA genetic polymorphisms with
            diabetes mellitus risk: a case-control study.
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Wong,D., Auguste,G., Lino Cardenas,C.L., Turner,A.W., Chen,Y.,
            Song,Y., Ma,L., Perry,R.N., Aherrahrou,R., Kuppusamy,M., Yang,C.,
            Mosquera,J.V., Dube,C.J., Khan,M.D., Palmore,M., Kalra,J.,
            Kavousi,M., Peyser,P.A., Matic,L., Hedin,U., Manichaikul,A.,
            Sonkusare,S.K., Civelek,M., Kovacic,J.C., Bjorkegren,J.L.M.,
            Malhotra,R. and Miller,C.L.
  TITLE     FHL5 Controls Vascular Disease-Associated Gene Programs in Smooth
            Muscle Cells
  JOURNAL   Circ Res 132 (9), 1144-1161 (2023)
   PUBMED   37017084
  REMARK    GeneRIF: FHL5 Controls Vascular Disease-Associated Gene Programs in
            Smooth Muscle Cells.
REFERENCE   3  (bases 1 to 3949)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 3949)
  AUTHORS   Jiang,Z., Zhao,L., Zhang,X., Zhang,W., Feng,Y. and Li,T.
  TITLE     Common variants in KCNK5 and FHL5 genes contributed to the
            susceptibility of migraine without aura in Han Chinese population
  JOURNAL   Sci Rep 11 (1), 6807 (2021)
   PUBMED   33762637
  REMARK    GeneRIF: Common variants in KCNK5 and FHL5 genes contributed to the
            susceptibility of migraine without aura in Han Chinese population.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3949)
  AUTHORS   Macho,B., Brancorsini,S., Fimia,G.M., Setou,M., Hirokawa,N. and
            Sassone-Corsi,P.
  TITLE     CREM-dependent transcription in male germ cells controlled by a
            kinesin
  JOURNAL   Science 298 (5602), 2388-2390 (2002)
   PUBMED   12493914
REFERENCE   6  (bases 1 to 3949)
  AUTHORS   Peri,A., Luciani,P., Tonacchera,M., Agretti,P., Baglioni-Peri,S.,
            Buci,L., Conforti,B., Cioppi,F., Biliotti,G., Serio,M., Vitti,P.
            and Chiovato,L.
  TITLE     Expression of cAMP-responsive element binding protein and inducible
            cAMP early repressor in hyperfunctioning thyroid adenomas
  JOURNAL   Eur J Endocrinol 146 (6), 759-766 (2002)
   PUBMED   12039695
  REMARK    GeneRIF: No difference was found in the levels of ICER transcripts
            in thyroid adenomas and surrounding normal tissue (obtained at
            surgery), independently of the TSH receptor gene status.
REFERENCE   7  (bases 1 to 3949)
  AUTHORS   Fimia,G.M., Morlon,A., Macho,B., De Cesare,D. and Sassone-Corsi,P.
  TITLE     Transcriptional cascades during spermatogenesis: pivotal role of
            CREM and ACT
  JOURNAL   Mol Cell Endocrinol 179 (1-2), 17-23 (2001)
   PUBMED   11420126
  REMARK    Review article
REFERENCE   8  (bases 1 to 3949)
  AUTHORS   Palermo,I., Litrico,L., Emmanuele,G., Giuffrida,V.,
            Sassone-Corsi,P., De Cesare,D., Maria Fimia,G., D'Agata,R.,
            Calogero,A.E. and Travali,S.
  TITLE     Cloning and expression of activator of CREM in testis in human
            testicular tissue
  JOURNAL   Biochem Biophys Res Commun 283 (2), 406-411 (2001)
   PUBMED   11327716
REFERENCE   9  (bases 1 to 3949)
  AUTHORS   Fimia,G.M., De Cesare,D. and Sassone-Corsi,P.
  TITLE     A family of LIM-only transcriptional coactivators: tissue-specific
            expression and selective activation of CREB and CREM
  JOURNAL   Mol Cell Biol 20 (22), 8613-8622 (2000)
   PUBMED   11046156
REFERENCE   10 (bases 1 to 3949)
  AUTHORS   Fimia,G.M., De Cesare,D. and Sassone-Corsi,P.
  TITLE     CBP-independent activation of CREM and CREB by the LIM-only protein
            ACT
  JOURNAL   Nature 398 (6723), 165-169 (1999)
   PUBMED   10086359
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK223539.1, AL132776.11 and
            BX118342.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001322466.1.
            
            Summary: The protein encoded by this gene is coordinately expressed
            with activator of cAMP-responsive element modulator (CREM). It is
            associated with CREM and confers a powerful transcriptional
            activation function. CREM acts as a transcription factor essential
            for the differentiation of spermatids into mature spermatozoa.
            There are multiple polyadenylation sites found in this gene.
            Polymorphisms in this gene may be associated with susceptibility
            for migraine headaches. Alternative splicing results in multiple
            transcript variants encoding the same protein. [provided by RefSeq,
            Apr 2016].
            
            Transcript Variant: This variant (3) differs in the 5' UTR,
            compared to variant 1. Variants 1, 2, 3 and 4 all encode the same
            protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.262685.1, AK057611.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000450218.6/ ENSP00000396390.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1156              AK223539.1         84-1239
            1157-3476           AL132776.11        78345-80664
            3477-3949           BX118342.1         165-637
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q16.1"
     gene            1..3949
                     /gene="FHL5"
                     /gene_synonym="1700027G07Rik; ACT; dJ393D12.2; FHL-5"
                     /note="four and a half LIM domains 5"
                     /db_xref="GeneID:9457"
                     /db_xref="HGNC:HGNC:17371"
                     /db_xref="MIM:605126"
     exon            1..228
                     /gene="FHL5"
                     /gene_synonym="1700027G07Rik; ACT; dJ393D12.2; FHL-5"
                     /inference="alignment:Splign:2.1.0"
     exon            229..399
                     /gene="FHL5"
                     /gene_synonym="1700027G07Rik; ACT; dJ393D12.2; FHL-5"
                     /inference="alignment:Splign:2.1.0"
     CDS             241..1095
                     /gene="FHL5"
                     /gene_synonym="1700027G07Rik; ACT; dJ393D12.2; FHL-5"
                     /note="LIM protein ACT; activator of cAMP-responsive
                     element modulator (CREM) in testis; activator of
                     cAMP-responsive element modulator in testis"
                     /codon_start=1
                     /product="four and a half LIM domains protein 5"
                     /protein_id="NP_001309395.1"
                     /db_xref="CCDS:CCDS5035.1"
                     /db_xref="GeneID:9457"
                     /db_xref="HGNC:HGNC:17371"
                     /db_xref="MIM:605126"
                     /translation="MTTAHFYCQYCTASLLGKKYVLKDDSPYCVTCYDRVFSNYCEEC
                     KKPIESDSKDLCYKDRHWHEGCFKCTKCNHSLVEKPFAAKDERLLCTECYSNECSSKC
                     FHCKRTIMPGSRKMEFKGNYWHETCFVCENCRQPIGTKPLISKESGNYCVPCFEKEFA
                     HYCNFCKKVITSGGITFCDQLWHKECFLCSGCRKDLCEEQFMSRDDYPFCVDCYNHLY
                     ANKCVACSKPISGLTGAKFICFQDSQWHSECFNCGKCSVSLVGKGFLTQNKEIFCQKC
                     GSGMDTDI"
     exon            400..574
                     /gene="FHL5"
                     /gene_synonym="1700027G07Rik; ACT; dJ393D12.2; FHL-5"
                     /inference="alignment:Splign:2.1.0"
     exon            575..744
                     /gene="FHL5"
                     /gene_synonym="1700027G07Rik; ACT; dJ393D12.2; FHL-5"
                     /inference="alignment:Splign:2.1.0"
     exon            745..931
                     /gene="FHL5"
                     /gene_synonym="1700027G07Rik; ACT; dJ393D12.2; FHL-5"
                     /inference="alignment:Splign:2.1.0"
     exon            932..3949
                     /gene="FHL5"
                     /gene_synonym="1700027G07Rik; ACT; dJ393D12.2; FHL-5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1140..1145
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FHL5"
                     /gene_synonym="1700027G07Rik; ACT; dJ393D12.2; FHL-5"
                     /note="hexamer: ACTAAA"
     polyA_site      1156
                     /gene="FHL5"
                     /gene_synonym="1700027G07Rik; ACT; dJ393D12.2; FHL-5"
                     /note="major polyA site"
     regulatory      1942..1947
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FHL5"
                     /gene_synonym="1700027G07Rik; ACT; dJ393D12.2; FHL-5"
                     /note="hexamer: AATAGA"
     polyA_site      1961
                     /gene="FHL5"
                     /gene_synonym="1700027G07Rik; ACT; dJ393D12.2; FHL-5"
                     /note="major polyA site"
     regulatory      3149..3154
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FHL5"
                     /gene_synonym="1700027G07Rik; ACT; dJ393D12.2; FHL-5"
                     /note="hexamer: ATTAAA"
     polyA_site      3176
                     /gene="FHL5"
                     /gene_synonym="1700027G07Rik; ACT; dJ393D12.2; FHL-5"
                     /note="major polyA site"
     regulatory      3917..3922
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FHL5"
                     /gene_synonym="1700027G07Rik; ACT; dJ393D12.2; FHL-5"
                     /note="hexamer: AATAAG"
     polyA_site      3949
                     /gene="FHL5"
                     /gene_synonym="1700027G07Rik; ACT; dJ393D12.2; FHL-5"
ORIGIN      
        1 agtaggagtc atgtacacgg tataagactt ggaggaaatg cagggaccct tctgttttct
       61 cctgcaaagt tgaaaaagct gcatgcagtt ggaagggatc ttcagatggt gctggtgact
      121 ttctgttcct tttggagttg acatgcatgt ggattggagg aaaaataatc aacatatatt
      181 ctcctttcgt actgtttaaa tcacaggaag aagcggcttt aagacaaaga tcaaaccaaa
      241 atgacaactg ctcactttta ctgtcaatac tgcacagcat cacttcttgg gaagaaatat
      301 gtactaaagg atgacagtcc atactgtgtt acatgttatg atcgtgtatt ttctaactat
      361 tgcgaggaat gcaaaaaacc aattgaatct gattctaagg atctttgtta caaagaccgg
      421 cactggcatg aaggatgctt caagtgcacc aaatgcaatc actctttggt ggaaaagcct
      481 tttgctgcca aggatgagcg cctgctgtgc acggagtgct attctaacga gtgctcctcc
      541 aagtgcttcc actgcaagag gaccatcatg cctggttccc gcaaaatgga atttaaggga
      601 aactactggc atgaaacctg ttttgtgtgt gagaattgcc gacaacctat agggacaaag
      661 cctttgatct ccaaagagag tggcaattat tgtgtgccat gttttgagaa ggagtttgct
      721 cactactgca acttttgtaa gaaggtgata acttcaggtg ggataacatt ttgtgaccag
      781 ctatggcata aagagtgttt tctgtgtagt ggctgtagga aagatctctg tgaagaacag
      841 ttcatgtcca gagacgacta tccattctgc gtggactgct acaaccatct ttatgccaac
      901 aagtgtgtag cctgttccaa acccattagt ggtctcacag gtgccaagtt tatctgcttt
      961 caagacagcc agtggcatag cgaatgcttt aactgcggga aatgctctgt ctccttggtg
     1021 ggtaaaggct tcctgaccca gaacaaggaa atcttctgcc aaaaatgtgg ctccggaatg
     1081 gacactgaca tctaggagac agtccttgcc cacctaaaat ccattttgcc ttcgttgtca
     1141 ctaaagccag aactcagttg cggtcttatt tttgacttaa gttttagaaa atattcatgt
     1201 agtttagagt ggaaaagttt ttgcacacat tttgatcgaa ctatattcta agcacacaaa
     1261 aagcacagta gaacagaaat gaatatatgc taaatcacaa agacaactct ccttgcaaaa
     1321 tactgcactc tgctgttaga agcagaggaa aatatctctt cataatcaaa tatatgacat
     1381 atacctagga gctgtggatg taattactga agaatgaggt ttttcatacc tgaagagtaa
     1441 aagaaaaact aagagatcaa actgagaggt atacatgagg ctagtgtttg cattcctggt
     1501 tatatgtaat gtgataaatt agctatgaat ggtaaaagaa caaaactgat cagagtaaat
     1561 tcattagcaa ttatcacatt taagaattgt tatcatttaa aaatcatcct ttttttcaca
     1621 catactgact gtagaattaa aaaacaaagc tacaatttgc cctcatacac actgcttcca
     1681 cagcttgcta gttctgtgtt tactcaatgg aatatgagaa ctgaggacat tatggctctc
     1741 ataatctgca taggtataaa gaggatcatt tctacctagg gatagccaaa gcatgggttt
     1801 caacacgttt tgtattaaat atattttcaa agtccacagt gatttttcta aacgcttaca
     1861 gattccatga aataaaagtc tattctttca agtggagcta accaggataa tctttgtccc
     1921 caaaggcaaa cctttaaaat aaatagatca ttaaatcttc acttgtggaa atgagtttta
     1981 gggaagggat tgagtcttgg aacatgactt tcatccagat atctaacgac ataatagaat
     2041 gcataattag ctctgtgcct catgcattat tgccttctaa aattacataa atgattaatg
     2101 ggcagacata aaagtgggct ttaaagaaat ctagtaataa taaaagctga aaaagaataa
     2161 ttcactgctg ataattcaga acctgcaaag cagcacaata gagtaatgtg ggctgtaggc
     2221 gagccctccc cactcttccc ccacacctgt gccacacccc ccttacccca agccagttgt
     2281 gcccccagtt tcaatctcct gcataaccag gaggtgcact cctaacccta agcaactgtc
     2341 ctataagtca gtcagtgttt attgatcaca caggaagtaa agagccttag tgagaagtcc
     2401 atcaccaggt ttatctttat accagacttt aactgatcat gaccctatct tgctacccct
     2461 ggatattttc cacattactt gggacaaatg gaaagcaacc tctgctctcc atgatctgtt
     2521 ttcacattgg ttatagcatc tcttggatgg agtagaggga ggtcaggaga agaagcccac
     2581 atagatatat aactgtcttc tctcagatta cacattatag cattaaaggt tttgattaaa
     2641 atctaattgt aagatactgc tgattacaat taccaaaata gcatcacagg agattcaatt
     2701 aaaattgtta tggaaataat ttttgaaata gtttaaaatg caagaatatt cagcgatgtt
     2761 aataatttag agatactaat aagtgttctg ctagatagac tatttgaaaa ctataaatgt
     2821 atttttggat taacaatgcc aaggtatttt ttcatattca cattgaaaaa tacaggcaaa
     2881 actatcaata ttttttctgc attatatgaa atttcacagg tagaactaaa tctatgatat
     2941 agaaaataat agcacattac acatagagaa aaccaagcca cttaggttta gatagatata
     3001 taacatgtaa catcttctct gttaaccaat tatagataca tatacaacat gtaatgtttt
     3061 cttagttaaa caattggaaa tgtttaggca ttttactgtg tgggggcagt gagtatgcac
     3121 atgatcagac ttgtattcta gctttacaat taaaattcac agaatcattt cctaaagatt
     3181 ttattatcct ttcataagta taaatatata tacttttaca tataaatagt tctgcattta
     3241 gaaggtatcc tacacatcaa acatccatta aacatgtact atgccaaaag ttagagctta
     3301 tactgttgtg ggtctggagt tgtaggagga tggatcatgg tatggaaatg cagaagtgcc
     3361 tgatgcaaag atccttgcct gcaggttaaa caaagcacca catatgtaga catagcacac
     3421 atatgcaaaa caactaagaa aaagacctcc ttaggtcttt gcgtaacaca cacaaagata
     3481 aatgtaaaaa ttatatagtt gatttatatg gtttatgtaa caaagagaaa aactccttaa
     3541 tggacttatc caaagatgaa atggactgac tcaggaaaca gtgggctccc agtaattcaa
     3601 aggtgacatc tgcatgggca ggaaagaagt taaaagcaaa tcccatgtgc caataacaac
     3661 cagatggctt ggaagtagca gagactgccg aagagcagga gaaaataaga ttgctaaaag
     3721 agaaagagtc agaggaaggg agaatattaa ctcttaggat aaaaagtgac ataaccggcc
     3781 atagaaagtt accaactgtt cataagtaaa ggaagaaaat aattattgta gtgtttgagg
     3841 aaaattatgc ttttgatcct ttgtggaata gaaccaaaga aagcaaggtt ccaaggatta
     3901 agtttcttac tgaaggaata agtttggtta atggctacaa aagtgaaaa
//
