LOCUS       NM_004468               1656 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens four and a half LIM domains 3 (FHL3), transcript
            variant 1, mRNA.
ACCESSION   NM_004468
VERSION     NM_004468.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Eshibona,N., Giwa,A., Rossouw,S.C., Gamieldien,J., Christoffels,A.
            and Bendou,H.
  TITLE     Upregulation of FHL1, SPNS3, and MPZL2 predicts poor prognosis in
            pediatric acute myeloid leukemia patients with FLT3-ITD mutation
  JOURNAL   Leuk Lymphoma 63 (8), 1897-1906 (2022)
   PUBMED   35249471
  REMARK    GeneRIF: Upregulation of FHL1, SPNS3, and MPZL2 predicts poor
            prognosis in pediatric acute myeloid leukemia patients with
            FLT3-ITD mutation.
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1656)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 1656)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1656)
  AUTHORS   Mao,J., Sun,Z., Cui,Y., Du,N., Guo,H., Wei,J., Hao,Z. and Zheng,L.
  TITLE     PCBP2 promotes the development of glioma by regulating
            FHL3/TGF-beta/Smad signaling pathway
  JOURNAL   J Cell Physiol 235 (4), 3280-3291 (2020)
   PUBMED   31693182
  REMARK    GeneRIF: PCBP2 promotes the development of glioma by regulating
            FHL3/TGF-beta/Smad signaling pathway.
REFERENCE   6  (bases 1 to 1656)
  AUTHORS   Turner,J., Nicholas,H., Bishop,D., Matthews,J.M. and Crossley,M.
  TITLE     The LIM protein FHL3 binds basic Kruppel-like factor/Kruppel-like
            factor 3 and its co-repressor C-terminal-binding protein 2
  JOURNAL   J Biol Chem 278 (15), 12786-12795 (2003)
   PUBMED   12556451
REFERENCE   7  (bases 1 to 1656)
  AUTHORS   Li,H.Y., Ng,E.K., Lee,S.M., Kotaka,M., Tsui,S.K., Lee,C.Y.,
            Fung,K.P. and Waye,M.M.
  TITLE     Protein-protein interaction of FHL3 with FHL2 and visualization of
            their interaction by green fluorescent proteins (GFP) two-fusion
            fluorescence resonance energy transfer (FRET)
  JOURNAL   J Cell Biochem 80 (3), 293-303 (2001)
   PUBMED   11135358
REFERENCE   8  (bases 1 to 1656)
  AUTHORS   Morgan,M.J. and Madgwick,A.J.
  TITLE     The LIM proteins FHL1 and FHL3 are expressed differently in
            skeletal muscle
  JOURNAL   Biochem Biophys Res Commun 255 (2), 245-250 (1999)
   PUBMED   10049693
REFERENCE   9  (bases 1 to 1656)
  AUTHORS   Lee,S.M., Tsui,S.K., Chan,K.K., Kotaka,M., Li,H.Y., Chim,S.S.,
            Waye,M.M., Fung,K.P. and Lee,C.Y.
  TITLE     Chromosomal mapping of a skeletal muscle specific LIM-only protein
            FHL3 to the distal end of the short arm of human chromosome 1
  JOURNAL   Somat Cell Mol Genet 24 (3), 197-202 (1998)
   PUBMED   10226657
REFERENCE   10 (bases 1 to 1656)
  AUTHORS   Morgan,M.J. and Madgwick,A.J.
  TITLE     Slim defines a novel family of LIM-proteins expressed in skeletal
            muscle
  JOURNAL   Biochem Biophys Res Commun 225 (2), 632-638 (1996)
   PUBMED   8753811
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL603790.41.
            
            On Nov 23, 2018 this sequence version replaced NM_004468.4.
            
            Summary: The protein encoded by this gene is a member of a family
            of proteins containing a four-and-a-half LIM domain, which is a
            highly conserved double zinc finger motif. The encoded protein has
            been shown to interact with the cancer developmental regulators
            SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein
            MyoD, and the high-affinity IgE beta chain regulator MZF-1. This
            protein may be involved in tumor suppression, repression of MyoD
            expression, and repression of IgE receptor expression. Two
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC001351.2, SRR1163655.14275.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373016.4/ ENSP00000362107.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-158               AL603790.41        77476-77633         c
            159-334             AL603790.41        71376-71551         c
            335-509             AL603790.41        71093-71267         c
            510-679             AL603790.41        70082-70251         c
            680-866             AL603790.41        69803-69989         c
            867-1656            AL603790.41        68889-69678         c
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p34.3"
     gene            1..1656
                     /gene="FHL3"
                     /gene_synonym="SLIM2"
                     /note="four and a half LIM domains 3"
                     /db_xref="GeneID:2275"
                     /db_xref="HGNC:HGNC:3704"
                     /db_xref="MIM:602790"
     exon            1..158
                     /gene="FHL3"
                     /gene_synonym="SLIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            159..334
                     /gene="FHL3"
                     /gene_synonym="SLIM2"
                     /inference="alignment:Splign:2.1.0"
     CDS             179..1021
                     /gene="FHL3"
                     /gene_synonym="SLIM2"
                     /note="isoform 1 is encoded by transcript variant 1; four
                     and a half LIM domains protein 3; LIM-only protein FHL3;
                     FHL-3; SLIM-2; skeletal muscle LIM-protein 2"
                     /codon_start=1
                     /product="four and a half LIM domains protein 3 isoform 1"
                     /protein_id="NP_004459.2"
                     /db_xref="CCDS:CCDS30678.1"
                     /db_xref="GeneID:2275"
                     /db_xref="HGNC:HGNC:3704"
                     /db_xref="MIM:602790"
                     /translation="MSESFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQ
                     QLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCS
                     ACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAP
                     RCARCSKTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSRDEDPYCVACFGELFA
                     PKCSSCKRPIVGLGGGKYVSFEDRHWHHNCFSCARCSTSLVGQGFVPDGDQVLCQGCS
                     QAGP"
     exon            335..509
                     /gene="FHL3"
                     /gene_synonym="SLIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            510..679
                     /gene="FHL3"
                     /gene_synonym="SLIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            680..866
                     /gene="FHL3"
                     /gene_synonym="SLIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            867..1656
                     /gene="FHL3"
                     /gene_synonym="SLIM2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1638..1643
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FHL3"
                     /gene_synonym="SLIM2"
                     /note="hexamer: AATAAA"
     polyA_site      1656
                     /gene="FHL3"
                     /gene_synonym="SLIM2"
                     /note="major polyA site"
ORIGIN      
        1 actcgcccac acggtccgtc tgcagtcggc agcctcgccg gcagctcgct cggcccccgc
       61 ttggcccggc ccgcaggtag gagccgccga gggacgcggc gcccggttcc cggccccgcc
      121 tgcgccgcgc gctccccgct cccgccgccc gcttcgaggg ttctctcccc ttgccaccat
      181 gagcgagtca tttgactgtg caaaatgcaa cgagtccctg tatggacgca agtacatcca
      241 gacagacagc ggcccctact gtgtgccctg ctatgacaat acctttgcca acacctgtgc
      301 tgagtgccag cagcttatcg ggcatgactc gagggagctg ttctatgaag accgccattt
      361 ccacgagggc tgcttccgct gctgccgctg ccagcgctca ctagccgatg aacccttcac
      421 ctgccaggac agtgagctgc tctgcaatga ctgctactgc agtgcgtttt cctcgcagtg
      481 ctccgcttgt ggggagactg tcatgcctgg gtcccggaag ctggaatatg gaggccagac
      541 atggcatgag cactgcttcc tgtgcagtgg ctgtgaacag ccactgggct cccgttcttt
      601 tgtgcccgac aagggtgctc actactgcgt gccctgctat gagaacaagt ttgctcctcg
      661 ctgcgcccgc tgcagcaaga cgctgacaca gggtggagtg acataccgtg atcagccgtg
      721 gcatcgagaa tgtctggtct gtaccggatg ccagacgccc ctggcagggc agcagttcac
      781 ctcccgggat gaagatccct actgtgtggc ctgttttgga gaactctttg cacctaagtg
      841 cagcagctgc aagcgcccca tcgtaggact cggtggaggc aagtatgtgt cctttgaaga
      901 ccgacactgg caccacaact gcttctcctg cgcccgctgc tctacctccc tggtgggcca
      961 gggcttcgta ccggatggag accaagtgct ctgccagggc tgtagccagg cagggcccta
     1021 agccagggct cctggaccca ggctttccca taccacgggc ccaggactgt ggctcctttt
     1081 ctaaaccacc tctgggactc agctcccccc gccaaaaaaa atgggtctcc ttctgggctc
     1141 caggattgtc tccccactcc agcatcccca aactggtact ccctgaccca gggccccaaa
     1201 cctgggctct tacagagcct ccatgagtca agccccctcc ccacacctgg actccagaat
     1261 tcaccctctc ccctgcagtc tgggttccca gactgagtcc tctccccaaa tcagggctct
     1321 agacccgagc cctccaaacc tggactctgg gacttaggcc cccttaaatc tagacttctc
     1381 tttataggtt tcaggtctcc tatgggtgcc tgggaagtcc ttgaaagtgg actgttctca
     1441 ggcttgacct gccccacccc atccccgcgg ttgaggctgt gggggcagca gatcaggagc
     1501 ccactgataa ggggccctag ggtacagggt gctgcccagc aggtcgccac cgagtgtctt
     1561 ctcattttat ttcagctcca ttttgcccat agatgggcag aggggtgaga ttggctcatc
     1621 ccccttccag attctgcaat aaagcggtgt gaggaa
//
