LOCUS       NM_001370298            8465 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens FYVE, RhoGEF and PH domain containing 4 (FGD4),
            transcript variant 9, mRNA.
ACCESSION   NM_001370298 XM_005253304
VERSION     NM_001370298.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8465)
  AUTHORS   Yang,Y., Jia,J., Sun,Z., Liu,C., Li,Z., Xiao,Y., Yu,J., Du,F.,
            Shi,Y., Sun,J., Shui,J. and Zhang,X.
  TITLE     Polymorphism of FGD4 and myelosuppression in patients with
            esophageal squamous cell carcinoma
  JOURNAL   Future Oncol 17 (18), 2351-2363 (2021)
   PUBMED   33709789
  REMARK    GeneRIF: Polymorphism of FGD4 and myelosuppression in patients with
            esophageal squamous cell carcinoma.
REFERENCE   2  (bases 1 to 8465)
  AUTHORS   Lin,J., Huang,H., Lin,L., Li,W. and Huang,J.
  TITLE     MiR-23a induced the activation of CDC42/PAK1 pathway and cell cycle
            arrest in human cov434 cells by targeting FGD4
  JOURNAL   J Ovarian Res 13 (1), 90 (2020)
   PUBMED   32772928
  REMARK    GeneRIF: MiR-23a induced the activation of CDC42/PAK1 pathway and
            cell cycle arrest in human cov434 cells by targeting FGD4.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8465)
  AUTHORS   Dai,X., Liu,J., Guo,X., Cheng,A., Deng,X., Guo,L. and Wang,Z.
  TITLE     Circular RNA circFGD4 suppresses gastric cancer progression via
            modulating miR-532-3p/APC/beta-catenin signalling pathway
  JOURNAL   Clin Sci (Lond) 134 (13), 1821-1839 (2020)
   PUBMED   32633323
  REMARK    GeneRIF: Circular RNA circFGD4 suppresses gastric cancer
            progression via modulating miR-532-3p/APC/beta-catenin signalling
            pathway.
REFERENCE   4  (bases 1 to 8465)
  AUTHORS   Mortreux,J., Bacquet,J., Boyer,A., Alazard,E., Bellance,R.,
            Giguet-Valard,A.G., Cerino,M., Krahn,M., Audic,F., Chabrol,B.,
            Laugel,V., Desvignes,J.P., Beroud,C., Nguyen,K., Verschueren,A.,
            Levy,N., Attarian,S., Delague,V., Missirian,C. and Bonello-Palot,N.
  TITLE     Identification of novel pathogenic copy number variations in
            Charcot-Marie-Tooth disease
  JOURNAL   J Hum Genet 65 (3), 313-323 (2020)
   PUBMED   31852984
  REMARK    GeneRIF: Identification of novel pathogenic copy number variations
            in Charcot-Marie-Tooth disease.
REFERENCE   5  (bases 1 to 8465)
  AUTHORS   Rabizadeh,S. and Bredesen,D.E.
  TITLE     Ten years on: mediation of cell death by the common neurotrophin
            receptor p75(NTR)
  JOURNAL   Cytokine Growth Factor Rev 14 (3-4), 225-239 (2003)
   PUBMED   12787561
  REMARK    Review article
REFERENCE   6  (bases 1 to 8465)
  AUTHORS   Salehi,A.H., Xanthoudakis,S. and Barker,P.A.
  TITLE     NRAGE, a p75 neurotrophin receptor-interacting protein, induces
            caspase activation and cell death through a JNK-dependent
            mitochondrial pathway
  JOURNAL   J Biol Chem 277 (50), 48043-48050 (2002)
   PUBMED   12376548
REFERENCE   7  (bases 1 to 8465)
  AUTHORS   Harrington,A.W., Kim,J.Y. and Yoon,S.O.
  TITLE     Activation of Rac GTPase by p75 is necessary for c-jun N-terminal
            kinase-mediated apoptosis
  JOURNAL   J Neurosci 22 (1), 156-166 (2002)
   PUBMED   11756498
REFERENCE   8  (bases 1 to 8465)
  AUTHORS   Delague,V.
  TITLE     Charcot-Marie-Tooth Neuropathy Type 4H - RETIRED CHAPTER, FOR
            HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   23926620
REFERENCE   9  (bases 1 to 8465)
  AUTHORS   Bird,T.D.
  TITLE     Charcot-Marie-Tooth Neuropathy Type 4 - RETIRED CHAPTER, FOR
            HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301641
REFERENCE   10 (bases 1 to 8465)
  AUTHORS   Bird,T.D.
  TITLE     Charcot-Marie-Tooth Hereditary Neuropathy Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301532
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC087245.29, AC090440.16 and
            AC084824.20.
            
            On Jun 1, 2020 this sequence version replaced NM_001370298.2.
            
            Summary: This gene encodes a protein that is involved in the
            regulation of the actin cytoskeleton and cell shape. This protein
            contains an actin filament-binding domain, which together with its
            Dbl homology domain and one of its pleckstrin homology domains, can
            form microspikes. This protein can activate MAPK8 independently of
            the actin filament-binding domain, and it is also involved in the
            activation of CDC42 via the exchange of bound GDP for free GTP. The
            activation of CDC42 also enables this protein to play a role in
            mediating the cellular invasion of Cryptosporidium parvum, an
            intracellular parasite that infects the gastrointestinal tract.
            Mutations in this gene can cause Charcot-Marie-Tooth disease type
            4H (CMT4H), a disorder of the peripheral nervous system. Multiple
            alternatively spliced transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Jan
            2015].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.2031614.1,
                                           SRR14038196.3511848.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000534526.7/
                                                ENSP00000449273.1
            RefSeq Select criteria           :: based on manual assertion,
                                                conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-402               AC087245.29        40588-40989
            403-555             AC090440.16        55403-55555
            556-739             AC090440.16        67532-67715
            740-1247            AC090440.16        73226-73733
            1248-1337           AC090440.16        89763-89852
            1338-1483           AC090440.16        92544-92689
            1484-1640           AC090440.16        93427-93583
            1641-1779           AC090440.16        99223-99361
            1780-1838           AC090440.16        102042-102100
            1839-1985           AC090440.16        102403-102549
            1986-2158           AC090440.16        110964-111136
            2159-2189           AC090440.16        115688-115718
            2190-2282           AC090440.16        116242-116334
            2283-2408           AC090440.16        116920-117045
            2409-2549           AC090440.16        124815-124955
            2550-2690           AC090440.16        129921-130061
            2691-5777           AC090440.16        131542-134628
            5778-8465           AC084824.20        2005-4692
FEATURES             Location/Qualifiers
     source          1..8465
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p11.21"
     gene            1..8465
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /note="FYVE, RhoGEF and PH domain containing 4"
                     /db_xref="GeneID:121512"
                     /db_xref="HGNC:HGNC:19125"
                     /db_xref="MIM:611104"
     exon            1..402
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     CDS             237..2948
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /note="isoform 8 is encoded by transcript variant 9;
                     actin-filament binding protein frabin; FGD1 family, member
                     4; FGD1-related F-actin-binding protein; zinc finger FYVE
                     domain-containing protein 6"
                     /codon_start=1
                     /product="FYVE, RhoGEF and PH domain-containing protein 4
                     isoform 8"
                     /protein_id="NP_001357227.2"
                     /db_xref="CCDS:CCDS91680.1"
                     /db_xref="GeneID:121512"
                     /db_xref="HGNC:HGNC:19125"
                     /db_xref="MIM:611104"
                     /translation="MSDEGGSNFRRVAIRRKSNPSYLPPGVPRPWSRPASHLGRVGTA
                     AFKGQVPSGATGSSTCPKIALVPPCSTSSTTTLVGENVSEEEAQGINGNRPAKHSAAS
                     PKPQVPPKPLHLQNSPSSNIHQTPRHKALPSAKPRMEEIKPASASCVSKEKPSKVSDL
                     ISRFEGGSSLSNYSDLKKESAVNLNAPRTPGRHGLTTTPQQKLLSQHLPQRQGNDTDK
                     TQGAQTCVANGVMAAQNQMECEEEKAATLSSDTSIQASEPLLDTHIVNGERDETATAP
                     ASPTTDSCDGNASDSSYRTPGIGPVLPLEERGAETETKVQERENGESPLELEQLDQHH
                     EMKETNEQKLHKIANELLLTERAYVNRLDLLDQVFYCKLLEEANRGSFPAEMVNKIFS
                     NISSINAFHSKFLLPELEKRMQEWETTPRIGDILQKLAPFLKMYGEYVKGFDNAMELV
                     KNMTERIPQFKSVVEEIQKQKICGSLTLQHHMLEPVQRIPRYEMLLKDYLRKLPPDSL
                     DWNDAKKSLEIISTAASHSNSAIRKMENLKKLLEIYEMLGEEEDIVNPSNELIKEGQI
                     LKLAARNTSAQERYLFLFNNMLLYCVPKFSLVGSKFTVRTRVGIDGMKIVETQNEEYP
                     HTFQVSGKERTLELQASSAQDKEEWIKALQETIDAFHQRHETFRNAIAKDNDIHSEVS
                     TAELGKRAPRWIRDNEVTMCMKCKEPFNALTRRRHHCRACGYVVCWKCSDYKAQLEYD
                     GGKLSKVCKDCYQIISGFTDSEEKKRKGILEIESAEVSGNSVVCSFLQYMEKSKPWQK
                     AWCVIPKQDPLVLYMYGAPQDVRAQATIPLLGYVVDEMPRSADLPHSFKLTQSKSVHS
                     FAADSEELKQKWLKVILLAVTGETPGGPNEHPATLDDHPEPKKKSEC"
     exon            403..555
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            556..739
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            740..1247
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1337
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1483
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1640
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1779
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..1838
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            1839..1985
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            1986..2158
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            2159..2189
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..2282
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            2283..2408
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            2409..2549
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            2550..2690
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            2691..8465
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8444..8449
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /note="hexamer: AATAAA"
     polyA_site      8465
                     /gene="FGD4"
                     /gene_synonym="CMT4H; FRABP; ZFYVE6"
                     /note="major polyA site"
ORIGIN      
        1 gcagataccg agacgctgcg actgccgcag gagtcgccgc agccaaactc gccgcgacgc
       61 cgggagggag cgtaccggga aggagaggga gaggaggcac tcgctgagga gacgccgcag
      121 tagagggcgc ccccggagtc gcgccgaacc tgggcatgca ggcgacgccc cccaggggcc
      181 gctcgcggct ggacgggagc gggaggagtc ggggagcggc ggtcgagccc ggcaggatga
      241 gcgacgaggg cggctccaac ttcaggcggg tggcgatccg gcggaagtcc aacccctcct
      301 acctgccgcc cggggtgccc cggccctgga gcaggcccgc gtcgcacctg ggacgtgtag
      361 ggaccgctgc cttcaagggc caggtgccct caggagccac aggcagcagt acctgtccaa
      421 agatcgcttt agttccacct tgctccacaa gcagcacaac cacactggtt ggtgagaatg
      481 tatctgaaga agaggctcag ggaataaatg ggaacaggcc agcaaaacac tcagctgcaa
      541 gtccaaagcc acaagtgcct ccaaagccat tacacctgca gaattcacct tcgtccaata
      601 tacaccaaac ccccaggcat aaagctttac ctagtgcaaa accaaggatg gaggaaatta
      661 aacctgcctc tgcttcttgt gtctcaaaag aaaaacccag taaggtatca gatctcatca
      721 gtcgctttga aggaggcagc tcattatcaa attatagtga tttgaagaaa gagtctgctg
      781 tgaacctaaa tgctcctaga accccaggaa ggcatggatt gacaaccaca cctcaacaaa
      841 aactcctctc ccagcacttg ccacagaggc agggaaatga tacagataag actcagggtg
      901 cacagacttg tgtggccaac ggtgtaatgg cagcacaaaa ccagatggaa tgtgaggagg
      961 agaaagctgc cactcttagc tcagatactt ctattcaagc ttctgaaccc ttgcttgata
     1021 cgcacatagt gaatggagaa agagatgaaa ctgccacagc tcctgcatca cccacaacag
     1081 acagctgtga tggaaatgct tctgacagta gctacaggac tccaggcata ggcccagtgc
     1141 tccccctaga agaaagaggg gcagaaacag aaaccaaggt acaagagagg gaaaatgggg
     1201 aaagccctct ggaactggag cagctggacc agcaccatga gatgaaggag actaatgagc
     1261 aaaaacttca caaaatagcc aatgaacttt tgcttactga aagagcttat gtcaaccgac
     1321 ttgacctctt agatcaggta ttttattgca aactgttgga agaagcaaac cgaggctcgt
     1381 ttccagcaga gatggtgaat aaaatctttt ctaatatttc atcaataaat gccttccata
     1441 gtaaattcct cttgccagag ctggagaaac gaatgcaaga atgggaaact actcctagaa
     1501 ttggagacat ccttcagaaa ttggcaccat tccttaagat gtatggagaa tatgtgaaag
     1561 gatttgataa tgcaatggaa ttggttaaaa acatgacaga acgtattccc cagttcaaat
     1621 cagtggttga agaaattcag aaacagaaaa tctgtgggag cttaactttg cagcatcaca
     1681 tgctagaacc tgttcagcgg attccccggt atgagatgct ccttaaggac tatctaagga
     1741 aattgcctcc tgattccctg gactggaatg atgctaaaaa atcacttgaa attatatcta
     1801 cagcagcaag ccattctaat agtgcaataa ggaaaatgga gaacctaaag aaactcttag
     1861 agatttatga aatgttggga gaagaagaag acattgtaaa cccttcaaat gaactaataa
     1921 aagaaggaca gatcctcaaa ctagctgctc ggaacacttc agcacaagaa cgctaccttt
     1981 tcttattcaa caacatgttg ctgtactgtg tgcccaaatt cagcttggta ggctctaaat
     2041 tcacagttcg aaccagggtt ggcattgatg gaatgaaaat tgtagagact caaaatgaag
     2101 aatatccaca tactttccag gtgtctggga aagagagaac actggaactg caggccagtt
     2161 ctgcgcaaga caaagaagaa tggatcaagg cccttcaaga aaccatcgat gcttttcatc
     2221 aaaggcatga aaccttcaga aatgcaattg caaaggataa tgacattcac tcagaggttt
     2281 ctactgctga gctagggaaa agagccccaa gatggatccg agataatgaa gtgacaatgt
     2341 gtatgaaatg taaagaacct ttcaatgcac tgacacgaag gaggcatcat tgtcgagcat
     2401 gtggatatgt ggtttgttgg aaatgctccg actacaaagc tcaacttgaa tatgatggtg
     2461 gtaaattgag caaagtttgt aaagactgtt atcaaatcat aagtggattc acagacagtg
     2521 aagaaaagaa aagaaaagga attttagaga ttgaatcagc agaagtatct ggaaacagtg
     2581 tggtgtgcag ctttcttcag tatatggaga agtcaaaacc ttggcagaaa gcttggtgtg
     2641 tgatccccaa gcaagaccct cttgtgctgt acatgtatgg tgccccccag gacgtcagag
     2701 cccaggccac cattccactt ctgggctatg tggtggatga aatgccaagg agcgcagacc
     2761 tgccacacag tttcaaactg acccagtcta agtccgtgca cagctttgct gcagacagtg
     2821 aggaactgaa gcagaagtgg ctgaaagtca tccttttagc tgtcacaggt gagacaccag
     2881 gtggtccaaa tgagcatcca gccaccttgg atgatcatcc tgaacctaag aaaaaatcag
     2941 aatgctgaac tcctccagga ccagccatgg tgtggaggtc tcaggactta cagctcaaga
     3001 cattcccagc tcttcttaca catctgctag cactttatgt tgaaaaatat aggcccataa
     3061 atgcatcttt tgaggactat tttcctatgt ttatgtactc ttagtgaaat tagtgtgcag
     3121 agtcattcta ccgataaagt tttgaaataa tgtgaaaact ggagcatttt ttgagctatt
     3181 ccttgaatat gtgctttttt gtcttgaaga aatggtgtat caattgattc tgtcaccgtc
     3241 aggttagaat gagcacttcc atttaagaaa tcctttcatg tcttcttctc tttcacatgt
     3301 aggacctgga acagtttgaa agatatacct ccatgttgcc aaaatagatc catggtgaaa
     3361 aatacaggga cagttgaggc tattgtatta acttatttaa tttagtttat aaatctctag
     3421 ctgcataaat gatgtctgtt cttttaaaac aaaagaaaaa ggacaaattg ttggtgttca
     3481 gatttccgat ttataaagaa aagataactt gtttttgtag aaatactcct aagaatgtct
     3541 taagtatata gcaattatgt atatatagta ttaaatatat atattataca tgcttttagg
     3601 ttcacattca tctctaattt attttttaaa tataaagcat ggtttatcgt ggaattgagc
     3661 aatgttctaa actgaagaaa tgttttggtg atttatgttt tgctgattgg catttgaggg
     3721 tattgatatt tttataataa gcggtaattt atgtggcatg ggatatattt gtgaattcca
     3781 acagtacttt taaagtacca ttttttgttt ctgtgcctat ttaaaacagt gcctctctta
     3841 gaaggtgcta ttaatataag atgagggttc agttaatgct tcagtcttga ttattctaag
     3901 attaaagtgc attttggtca ctgggacagt caaagtcata caggttattt taccgtttac
     3961 aatcctataa ttattgcagt agttaacatc agcatgtaca aacattttca ccaaaaccca
     4021 aactttatga gacacctgac cttttacaga actaggatat gtggaaacca catttatcgt
     4081 aatgtttttc ttttccgtaa gataatcgag catggttaaa taaggtctct atataatgaa
     4141 cacttaagat aactgtatgg gccttttcct ttgtcggaat gaaacaaaat ccacatgtga
     4201 taacctatgt ttggggtggg ggctggtcac atgattcctt gtcatgaagg ctgctcttca
     4261 ctcttttgta cttgtgatta aaatctcaca ctaagtttac tatttaactt aatccttttt
     4321 ctcaaattaa accttttttt atactgtcat accccaaaca ttgtcacttt ttaatgtacc
     4381 agtctgctag aaatctacaa gaatgatccc actatcttta gtatgtaagg attacacctt
     4441 agatatagaa tagaatttga gactgccaca catttattca ggccttgtta ctttaagaaa
     4501 tatcttattt tttccacccc aaaggagcaa gtgaaccaat tattgctgac ataataggtt
     4561 cccttcctcc ataaaaggat tatcagccat gatcattaac attaaaccaa atagatggag
     4621 ccaaaaagag ataaaagtag cttcttaggc cctattaaat cagggaaaga ctagaagaaa
     4681 atttacattg cttctctttt ttaatggcca gcggggtatg tgtgtgatgt atactcagag
     4741 tggttttttg gaaaattaaa ctgtagctat agatgatgct ttggttttct taatctcaag
     4801 aaaagagaag caatggaaat acagaatgat taactgggga agaggacttt tgtaagtgtg
     4861 actagaacaa tggtaatgta tgggccaatg ggaagattta taagtcagat tatttttgaa
     4921 aactattttt aaaagcaaga agactacact ttccctacca aaacagaagt aacatggaca
     4981 cacttaagtt ttggctcaat aaatatcaat gttatttaac ttagatcatt gaaagaatac
     5041 tcatgtaaag ataatgttta ttatcatgct cttaacagtc attatcttta attttttaaa
     5101 tgagaagcat taatactaga agagaatttc tggttatccg gtcaccatat tcactttcca
     5161 ccccacattc ttcagctaaa cgcaaagaga agcagtgaaa cagccttacc cgcttctctc
     5221 ttattaaaga atcactgatg tttttactca atgaaaggta aagtaatacc cttagccatt
     5281 tattaaacaa ttcaaccaag agacctcaaa gtgtgattga tgataagaat aatcaatgcc
     5341 ttttcccttc caacatactt gagcagtcat gacaacctaa aaatatcatt ggttgctttc
     5401 ccattaaaac caacgttccc tgtgggtctt aattctttat tttcactcat ttggtgcttt
     5461 ccaagtcatc ttttctttaa agtgcccttc ctccaaactt taaaaagtac ttccttgaca
     5521 aaatttctat tcattataaa acatttcaat attttaagct taaattttgc ttttgctgaa
     5581 agcctaccat ttggcgtgtt aagtatgaaa tatagtgcag acttttatct tggttttaag
     5641 tggggctcaa taaaaaacac cagccacttt tgtaataatg gcatcacagt gtcatcatgt
     5701 atgcaagcaa taaaactctt tagggtatgg ttttatactg aaaatttaat atgaaggccc
     5761 cttcccacaa gaatatagat aattattaac tttctagatg tgatacggta attcgaattg
     5821 cagagtataa ggaagggaaa tgggaagggg catcattcct tggattttaa ataactatca
     5881 agaacatttt tactttaaca aagaaaatgg atagaaaaag cacattttgt tttccctgaa
     5941 gtttaatttg acatcaggtt tgtgtactta tcttcactag gtgacttaac ttaccccaat
     6001 ttttttaaaa attattaaac tttttacaga aactaacctt ttaaatgtac cctttcccca
     6061 tatatatata cgcacacgtt tggatttttt ttttttaaga acacttgttc tagttataaa
     6121 tatataaaga aaacgataaa gtttgtggta ctgcagggtt gttaaagatt ctttgatgcc
     6181 ttctaaaaac ttttgtcaaa aatacttttg agttcacaat tctgttttac ttttccttgt
     6241 ccttactttt tggaaacagg gtggttgctt ttatttgttt tctggttata ttcaaagcct
     6301 taagttctta atctgagcat attgtctgtg ataaatttct gatgatcttt ctggactaga
     6361 tacaacctga gtagcaagca ccaaccggag caagtaaact tctagggaac aagcgtcttg
     6421 ggttttatag gtatctttgc tataatgcag aatagattaa tgaagatttc ctatatcata
     6481 tgatatttgt gttagtgggt ctaagattaa gcacatgata tttataagct aaaattaact
     6541 caaaagtcaa gaatgtctta atgttttcat tcttaaattt tgtattctcc aagaatgtat
     6601 tagtatatga actgtggcca accagttcct attcttcaga ctgtattgac atctgtagtg
     6661 gatcatgttg cttcttcatt cttaccaatt ttattagaat caaacttctt gttatttgca
     6721 tactattatc tactatagat tctcagcttt agaaaatgac tatgatacat aaagaccact
     6781 aggtcaactt aaaaaaccct ttctgtgaat ttatacatgt atgtatatat gtaaaaacac
     6841 tgttgatttg caattctgtc tttccataga aatgaacttt ttctatcgaa attgtttaac
     6901 ttaaatattt taacataaat tatttacatg gatctttatg tataattcat ccttatatat
     6961 accccttaat cacgtagtca tgagaagata actttgcttt ctttacagaa agggcagaga
     7021 ggaataagat aagaaactga aacaagcaag aatgaagaga gatgtggggg agagactgct
     7081 ggtctccaga ccacagcaat gtgttttaag ataagatgaa atattttaac tgcagaaggg
     7141 atataaaatc tatgtaatta catgctgatg ggatccattg cacccaggtt tttgaccttg
     7201 gcctgtaaat gctagactat gatatcttgt tattcttttt ctcctttggg cttttaaaaa
     7261 ataatttcat tctcagatca ttttctgtac tgtttactga ggcaaaaaaa aaaaaaatct
     7321 gaagtcaatc atggtcttct actttctgga ctgagcattt ggcagaatgc agtatctttt
     7381 cctgtatttt gacatgaaat agcacatggc ttctacaaga tagttttaac ttgttggggt
     7441 caccgggagt tatatgatgg tcaacccctt ttcccaaaat tcattgtggt agttttagtg
     7501 gaaaacgtaa atcaagaaat ctcatatcat actttaataa ataaatacca aatacatagt
     7561 gacatatagg tttgggaaga aactagtctg tggggaccat tataagagaa tcacattata
     7621 tattacacag tatatggata ttggaatgta tcacttgtgg ggggttctct tcattagcaa
     7681 aacagtcatg tctgtctgta tataagactt tttttttttt aaccaaacta gcatttcatt
     7741 ttgtgagtga caattgacat tttaaaataa gcataggccg ggcatagtgg ctcatgcctg
     7801 taatcccagc ccttgggagg ccgaggtggg cagatcactt caggtcagga gtttgagacc
     7861 agcctggcca acatggtgaa accctgtctc tactaaataa aaataaaaca aaataagtgt
     7921 atctgggcca ggcacggtag cccatgcctg taatcccagc actttgggag gccgaggagt
     7981 tcgagaccag cctggccaac atggcaaaac cctgtctcta caaaaattag ccaggtgtgg
     8041 tggcacatgc ctgtaatccc agctactcgg gaggttgagg caggagaatt gcttgaaccc
     8101 gggagacaga ggttgcagtg agccgagatt gcaccactgc actccggcct gggtgacaga
     8161 gcaagacttc gactcaaaaa ataaaataag cgtatctgga tttttctttt gaattcttga
     8221 ctgataagtt tgagaatgta tgtttttgga attttacagt tgactcaagt gtgaaagtat
     8281 acacaagtaa tttttctttt ctagctatgt gaatgtaaaa tacttgcaga taatgtatat
     8341 attgtgtaat atatgtatat ttggtcataa aagcagataa ttggaaacat tgtaaaatta
     8401 agcactttaa ttcctattaa atattaaaca tttgtatctt gtaaataaac acatccttaa
     8461 attaa
//
