LOCUS       NM_005246              12044 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens FER tyrosine kinase (FER), transcript variant 1, mRNA.
ACCESSION   NM_005246
VERSION     NM_005246.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12044)
  AUTHORS   Zhang,Y., Xiong,X., Zhu,Q., Zhang,J., Chen,S., Wang,Y., Cao,J.,
            Chen,L., Hou,L., Zhao,X., Hao,P., Chen,J., Zhuang,M., Li,D. and
            Fan,G.
  TITLE     FER-mediated phosphorylation and PIK3R2 recruitment on IRS4
            promotes AKT activation and tumorigenesis in ovarian cancer cells
  JOURNAL   Elife 11, e76183 (2022)
   PUBMED   35550247
  REMARK    GeneRIF: FER-mediated phosphorylation and PIK3R2 recruitment on
            IRS4 promotes AKT activation and tumorigenesis in ovarian cancer
            cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12044)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 12044)
  AUTHORS   Mehazri,L., Shpungin,S., Bel,S. and Nir,U.
  TITLE     Loss of Fer Jeopardizes Metabolic Plasticity and Mitochondrial
            Homeostasis in Lung and Breast Carcinoma Cells
  JOURNAL   Int J Mol Sci 22 (7), 3387 (2021)
   PUBMED   33806191
  REMARK    GeneRIF: Loss of Fer Jeopardizes Metabolic Plasticity and
            Mitochondrial Homeostasis in Lung and Breast Carcinoma Cells.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 12044)
  AUTHORS   Marciano,O., Mehazri,L., Shpungin,S., Varvak,A., Zacksenhaus,E. and
            Nir,U.
  TITLE     Fer and FerT Govern Mitochondrial Susceptibility to Metformin and
            Hypoxic Stress in Colon and Lung Carcinoma Cells
  JOURNAL   Cells 10 (1), 97 (2021)
   PUBMED   33430475
  REMARK    GeneRIF: Fer and FerT Govern Mitochondrial Susceptibility to
            Metformin and Hypoxic Stress in Colon and Lung Carcinoma Cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 12044)
  AUTHORS   Makovski,A., Yaffe,E., Shpungin,S. and Nir,U.
  TITLE     Intronic promoter drives the BORIS-regulated expression of FerT in
            colon carcinoma cells
  JOURNAL   J Biol Chem 287 (9), 6100-6112 (2012)
   PUBMED   22223638
  REMARK    GeneRIF: Transcription of the ferT gene in CC cells was found to be
            driven by an intronic promoter residing in intron 10 of the fer
            gene and to be regulated by the Brother of the Regulator of
            Imprinted Sites (BORIS) transcription factor.
REFERENCE   6  (bases 1 to 12044)
  AUTHORS   Warrington,J.A., Hall,L.V., Hinton,L.M., Miller,J.N., Wasmuth,J.J.
            and Lovett,M.
  TITLE     Radiation hybrid map of 13 loci on the long arm of chromosome 5
  JOURNAL   Genomics 11 (3), 701-708 (1991)
   PUBMED   1663488
  REMARK    Erratum:[Genomics 1992 Nov;14(3):832]
REFERENCE   7  (bases 1 to 12044)
  AUTHORS   Nishisho,I., Nakamura,Y., Miyoshi,Y., Miki,Y., Ando,H., Horii,A.,
            Koyama,K., Utsunomiya,J., Baba,S. and Hedge,P.
  TITLE     Mutations of chromosome 5q21 genes in FAP and colorectal cancer
            patients
  JOURNAL   Science 253 (5020), 665-669 (1991)
   PUBMED   1651563
REFERENCE   8  (bases 1 to 12044)
  AUTHORS   Hao,Q.L., Ferris,D.K., White,G., Heisterkamp,N. and Groffen,J.
  TITLE     Nuclear and cytoplasmic location of the FER tyrosine kinase
  JOURNAL   Mol Cell Biol 11 (2), 1180-1183 (1991)
   PUBMED   1990274
REFERENCE   9  (bases 1 to 12044)
  AUTHORS   Pawson,T., Letwin,K., Lee,T., Hao,Q.L., Heisterkamp,N. and
            Groffen,J.
  TITLE     The FER gene is evolutionarily conserved and encodes a widely
            expressed member of the FPS/FES protein-tyrosine kinase family
  JOURNAL   Mol Cell Biol 9 (12), 5722-5725 (1989)
   PUBMED   2685575
REFERENCE   10 (bases 1 to 12044)
  AUTHORS   Hao,Q.L., Heisterkamp,N. and Groffen,J.
  TITLE     Isolation and sequence analysis of a novel human tyrosine kinase
            gene
  JOURNAL   Mol Cell Biol 9 (4), 1587-1593 (1989)
   PUBMED   2725517
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from J03358.1, AK315234.1,
            AC116428.1 and BQ009328.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_005246.3.
            
            Summary: The protein encoded by this gene is a member of the
            FPS/FES family of non-transmembrane receptor tyrosine kinases. It
            regulates cell-cell adhesion and mediates signaling from the cell
            surface to the cytoskeleton via growth factor receptors.
            Alternative splicing results in multiple transcript variants. A
            related pseudogene has been identified on chromosome X. [provided
            by RefSeq, Apr 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a, also known as
            p94-Fer or FerS).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: J03358.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000281092.9/ ENSP00000281092.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1623              J03358.1           76-1698
            1624-2778           AK315234.1         1387-2541
            2779-11382          AC116428.1         86381-94984
            11383-12044         BQ009328.1         16-677              c
FEATURES             Location/Qualifiers
     source          1..12044
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q21.3"
     gene            1..12044
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /note="FER tyrosine kinase"
                     /db_xref="GeneID:2241"
                     /db_xref="HGNC:HGNC:3655"
                     /db_xref="MIM:176942"
     exon            1..104
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            105..250
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            251..516
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     CDS             310..2778
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /EC_number="2.7.10.2"
                     /note="isoform a is encoded by transcript variant 1;
                     phosphoprotein NCP94; tyrosine-protein kinase Fer;
                     tyrosine kinase 3; proto-oncogene c-Fer; feline
                     encephalitis virus-related kinase FER; fujinami poultry
                     sarcoma/Feline sarcoma-related protein Fer; protein
                     phosphatase 1, regulatory subunit 74; fer (fps/fes
                     related) tyrosine kinase"
                     /codon_start=1
                     /product="tyrosine-protein kinase Fer isoform a"
                     /protein_id="NP_005237.2"
                     /db_xref="CCDS:CCDS4098.1"
                     /db_xref="GeneID:2241"
                     /db_xref="HGNC:HGNC:3655"
                     /db_xref="MIM:176942"
                     /translation="MGFGSDLKNSHEAVLKLQDWELRLLETVKKFMALRIKSDKEYAS
                     TLQNLCNQVDKESTVQMNYVSNVSKSWLLMIQQTEQLSRIMKTHAEDLNSGPLHRLTM
                     MIKDKQQVKKSYIGVHQQIEAEMIKVTKTELEKLKCSYRQLIKEMNSAKEKYKEALAK
                     GKETEKAKERYDKATMKLHMLHNQYVLALKGAQLHQNQYYDITLPLLLDSLQKMQEEM
                     IKALKGIFDEYSQITSLVTEEIVNVHKEIQMSVEQIDPSTEYNNFIDVHRTTAAKEQE
                     IEFDTSLLEENENLQANEIMWNNLTAESLQVMLKTLAEELMQTQQMLLNKEEAVLELE
                     KRIEESSETCEKKSDIVLLLSQKQALEELKQSVQQLRCTEAKFSAQKELLEQKVQEND
                     GKEPPPVVNYEEDARSVTSMERKERLSKFESIRHSIAGIIRSPKSALGSSALSDMISI
                     SEKPLAEQDWYHGAIPRIEAQELLKKQGDFLVRESHGKPGEYVLSVYSDGQRRHFIIQ
                     YVDNMYRFEGTGFSNIPQLIDHHYTTKQVITKKSGVVLLNPIPKDKKWILSHEDVILG
                     ELLGKGNFGEVYKGTLKDKTSVAVKTCKEDLPQELKIKFLQEAKILKQYDHPNIVKLI
                     GVCTQRQPVYIIMELVSGGDFLTFLRRKKDELKLKQLVKFSLDAAAGMLYLESKNCIH
                     RDLAARNCLVGENNVLKISDFGMSRQEDGGVYSSSGLKQIPIKWTAPEALNYGRYSSE
                     SDVWSFGILLWETFSLGVCPYPGMTNQQAREQVERGYRMSAPQHCPEDISKIMMKCWD
                     YKPENRPKFSELQKELTIIKRKLT"
     exon            517..690
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            691..790
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            791..974
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1112
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..1232
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1355
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1545
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1638
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..1842
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1843..1965
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..2022
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2138
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2139..2233
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2234..2357
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2358..2512
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2513..2635
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2636..12044
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2851..2856
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /note="hexamer: AATGAA"
     polyA_site      2870
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
     regulatory      12019..12024
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /note="hexamer: AATAAA"
     polyA_site      12044
                     /gene="FER"
                     /gene_synonym="p94-Fer; PPP1R74; TYK3"
                     /note="major polyA site"
ORIGIN      
        1 actcattttt caccaaattc ttttggtgaa ggacgcttca gaaacggcca tcactgaaga
       61 gcagacccgt ttgggttctc cacgcattct agactcccga agagctcatg ttttttggct
      121 agacctatga ccattttcgc tagacttcac tgcacgtttt ctcaagtatc ttctttgtcc
      181 ctaatgtgtg acacctcatc atggacacgc tactttagct aaggcatgac cagcaatgaa
      241 cagtagtaag atatgtgctg attagaaggc tcacttgtgc agtgtggagg ataaccagtg
      301 ccttacaaaa tggggtttgg gagtgacctg aagaattcac atgaagcagt gttaaaattg
      361 caagactggg aattacggtt actggaaaca gtaaagaaat ttatggccct gagaataaaa
      421 agtgataaag aatatgcatc tactttacag aacctttgta atcaagttga taaggaaagt
      481 actgtccaaa tgaattatgt cagcaacgta tccaagtctt ggctacttat gattcagcag
      541 acagaacaac ttagtaggat aatgaagaca catgcagagg acttgaactc tggaccttta
      601 cacaggctca ccatgatgat taaggacaag cagcaggtga agaaaagtta cataggtgtt
      661 catcagcaga tagaggcaga gatgatcaag gttaccaaaa cagaattgga gaagttaaaa
      721 tgcagctata gacaattaat aaaagaaatg aattctgcca aagagaaata taaagaagct
      781 ttagctaaag ggaaggaaac tgaaaaggcc aaggaacgat acgacaaagc cacaatgaaa
      841 cttcatatgt tgcacaatca gtatgtattg gcgttgaaag gggcacagct ccatcagaat
      901 cagtattatg atatcacact tcccctgctt ctggactcct tacaaaagat gcaagaagaa
      961 atgataaaag cactcaaagg tatatttgat gaatacagcc agataaccag tcttgtcaca
     1021 gaggaaatag tgaatgtcca taaagagatt caaatgtcgg ttgaacagat agatcctagt
     1081 acagaataca ataatttcat agatgttcac agaacaacgg ctgctaaaga acaagaaata
     1141 gagtttgata cttccttact ggaagaaaat gaaaatcttc aggcaaatga gatcatgtgg
     1201 aataacttaa cagcagaaag tttgcaagta atgttgaaaa cgttagcgga agaacttatg
     1261 caaacacagc agatgctttt aaacaaggag gaggctgttt tggagttaga gaagagaatt
     1321 gaagaatctt ctgaaacttg tgagaagaag tctgatattg tgcttctgct aagccaaaaa
     1381 caggcacttg aagaactgaa acagtcagtc cagcagctga gatgcactga agcaaagttt
     1441 tcagcacaga aggaattact agagcaaaaa gtgcaagaaa atgatgggaa agagccacct
     1501 ccagtagtaa attatgaaga agatgcacga tcagttacat ctatggaaag aaaggagagg
     1561 ctatccaaat ttgaatctat tcgtcattca attgctggaa taattaggtc tccaaaatct
     1621 gcactgggct cttcagcact ttctgatatg atctccatca gtgagaagcc tttggcagaa
     1681 caggactggt accatggtgc aattcccaga atagaagctc aagaactgtt aaaaaaacaa
     1741 ggagactttt tggtgcgaga gagtcatggg aaacctggtg aatatgtcct ttctgtatat
     1801 tctgatggac agaggagaca ttttatcata caatatgttg ataacatgta tcgattcgag
     1861 ggcactgggt tttcaaacat tcctcaactt atagatcatc actatacaac aaaacaggtc
     1921 atcactaaga aatcaggtgt agttctgctg aatcctattc ctaaggacaa gaaatggatt
     1981 ctcagtcatg aagatgtcat attgggagaa ttactgggca agggaaattt tggtgaagta
     2041 tataagggca cattaaagga taaaacttct gttgctgtta aaacatgtaa agaagatctt
     2101 cctcaggaat tgaaaataaa atttttacaa gaagccaaaa ttctcaagca atatgatcat
     2161 cccaatattg tcaaacttat aggagtttgc acacaaagac agcctgtcta catcattatg
     2221 gaactggttt caggaggtga tttcctcacc tttctgagaa ggaagaagga tgaactaaaa
     2281 ctcaaacagt tagtgaaatt ttcattagac gctgctgctg gtatgttgta tctcgagagt
     2341 aaaaactgta tacacaggga ccttgctgca agaaactgcc tggtaggtga aaataatgtt
     2401 ctgaaaatca gtgactttgg aatgtctcgt caagaggatg gtggagtgta ttcatcttct
     2461 ggcttaaagc agattcccat taaatggaca gcaccggaag ctcttaatta tgggagatac
     2521 agttcagaga gtgacgtgtg gagctttggc atccttctct gggagacctt cagcttaggg
     2581 gtttgtccgt accctggaat gacaaatcag caagcaagag agcaagtaga aagaggatac
     2641 cggatgtcag ctccccagca ctgtccagag gatatttcca aaatcatgat gaagtgttgg
     2701 gattataaac ctgaaaatcg ccctaagttc agtgaacttc agaaagagct cactatcatc
     2761 aagagaaaac tcacatagtg acaggatggc gccaaactca gccttcagga ctctgtcctc
     2821 cagcagagta acattattgt tctcattaac aatgaattta taccacatta ccttcgacag
     2881 tcttctacca ttatttttta ttaactgggt gttttaaaag tacgttccac ttgtaaaaag
     2941 tcaaaggcaa atttgttaaa gaaataggca gtcctaccaa gggctttctt agctaaccat
     3001 agcaatccta ccatttcagg ccattgcaaa tagaaaagca caggcttcta agtgtgtgaa
     3061 ggataaaaga tctatcctat ccttttcaca ccttgtttct acttcaggca cagtttgtag
     3121 gctgccatcc tatgccacag accctactcc gttggtgcta taaacagcat tggtaatgcc
     3181 atgtttgcag gacatgttcc aaccacagct ggctttcacc tctgccaaag caggattata
     3241 tttgtaaaca aacaaacaga attttatatt gggaattatt tggacctcct gagatttttc
     3301 ttttctttct tgccaggtat aagcccaatg taagaatcat catgctccta cattatttgt
     3361 aaggttgtca ttttcctcct cctttgtctg ggcatcagaa aacaatgcac gtaggtcggg
     3421 cacggtggct cacgcctgta atcccaggca tgtaatctca tgcctgggat tacaggcgtg
     3481 agccacagtg cccagccccc tccctccttt gggaggctga ggcaggcgga tcacctgagg
     3541 tcaggagttc gagaccagcc tggcttgcat ggtgaaaccc tgtctctacc aaaaaaaaaa
     3601 aaagaaacac acacacaacg catgaaacgt cgtcaaagtt agagttgagg cagttgcttt
     3661 tcaaggatgt acagagagct gtgaagcaag aaacagattt gaacaggagg gtgggctgga
     3721 gaacagagca gctaaagaat gattaacaga gtaaagaaga aagtgaaaat ataaaaccac
     3781 cttatcagag ttatgccttt ctaaacatct aaacaaacaa acattcacat attctattct
     3841 aaaaacacat aataatacat attctatttt atggtctgaa tttaccagaa aggtcctaag
     3901 aaaagtttca ggagttcgta gagtcctcgt aatggcaaca aaaattttga gagaaactgc
     3961 aaagcacaaa tccacagaaa taacagcata ctttcagtta agcattttca ccttagaggg
     4021 ggcattccaa aatgtaattt cctttatttg taccagtttt tagagctgtc agaatttcat
     4081 gattctagtt caccaacaca ttcacgtgtg tacatgcgcg tgcacacaca ccaccaaggt
     4141 gcaagacaga tgtgaatgaa gtagacagtc tagaagtcag gtgaatatta atgagaaata
     4201 tgacataggg acagccctcc catttctact tccaccttca ttgaccatta tactgccccc
     4261 tggacattct taccattagc tgacaggtat gcaagcagag tgctgaagat gtctcatgtg
     4321 aataaaatgt aactcactca ctcagttaaa actaggattc tgtatcttaa ctcttatcta
     4381 gggaacttac ttacttaccc catgatctgt atatagttgc ctctcaggat aaatgcatat
     4441 atagaagatg gtcatgctca gggctaaata tgttctggaa gccttttata agttctcagg
     4501 cctgtgtcct ggtaaacatt accttagtga ttatttggtc tgcattcatt ggaagagctt
     4561 agggatagaa aatgcctcct gccctctcca gctgtttgct gcctgtacca tcgcacagaa
     4621 ctcacaccag tactttagag tatgaaagat aatactgaag agaaaagtga cttgtatcta
     4681 acatttcaat ctccttgcag atttcataca tacttaatga ttagtacatt cagactaaag
     4741 ggaaatttct tttgatggca cgtactatgg taaatcaaag cgcaaactca gaagtgctat
     4801 ggtattgagg cacttaccaa ccttcaaaag taagagtaaa atgggatatt cctacccttt
     4861 tttttagtat ttctaagtga atctctgtaa tattctttgt ctgtttaaag aaaacagcct
     4921 cccatctcat agtggcttca tgctaagctc tcagcaagga gagagaatga tggttttggc
     4981 tttagtttta tgaacaagac ctaaagctta atcctgacat tctggtgtta agaatgaaca
     5041 cagcattgat atttcactta tccaggctgt tagaatttgc agttcacatc attactctgt
     5101 gcttaaattt aaaaagcttt tcaaaacatc gtgacatgtg tagcatttaa attccatttt
     5161 attctttgtc ctaaagcagg cagtggtata gttcaggatt aataacttat tgggagtctc
     5221 tcagtcatat agaatattaa atttaatata gtcacatgca ttaagaaaaa cttacgcaaa
     5281 cagtttttca ctgttaattt tgttgtgctt gaagcataat ttattcatcc cttctgtcac
     5341 tgataattaa ttattggaat tatatacttt aggaggctaa tccatgccca ggaagcacga
     5401 acatactgtt cagaatgtgc aagttgatgt agggaaagga agattcagaa atgtacaaaa
     5461 tcctgtgatt tgtttagtaa gaaaagagtt gtaaccaaaa tttcttctgg tccggatgta
     5521 attagtccag tcacaaatct ttgtgttgtt aatggattat tcaagatata tatatatatt
     5581 cttactactg catagttcct ctcataagtc cttaaattta ttaaaagggg gaggtaaaca
     5641 caagcagtct gtgtctcact ggtcactaag gggcctttat gagacagttt aggttgttca
     5701 tcatttcaca aggaaagaaa tttttcactg ttaaattaac atttattacc aacatgtgaa
     5761 acctaaaatt aattttgcat tccatgtaat ctaaggccaa agccaacata ttctttctct
     5821 tctgggaaaa taaaattgac aggttttgtt taaacttcag aaactgaagc tttatttcag
     5881 cctgtcctgc catgaggtct ccagtgagag gtctccagtg aaaggtctca tgtaattgtt
     5941 tctcttttat ctcccaaatt taatgtgggc aataaaattc tcctttcgta attcactgtt
     6001 ttattacaaa tacgatatta ctgaaacatc ttattagcag ataagtatag accaggagcc
     6061 taactgtgtt agatattttc agtgcacatt tcacagttgt ttttcatttt aggattattt
     6121 tgagaagaaa aaatgttttg taattgaaat agttttaatt tcctttaaac cataatttgg
     6181 tttttctatc tatattatat tcagttcagt tttcccatat aacttttgtg aagattggct
     6241 agtaccacaa aataactatg gcttcacaat actagtgtta gattatgtca agaagaaagt
     6301 gttttagtta caacatacgc cacccactga ggaccttgta gtgggataaa caaaaattaa
     6361 aatgcaacaa tccaaataaa ttataatgta acaatacaaa tatctgaatc ataagtcaca
     6421 gtttttcagg gttctttcag tcacaccact ttataattaa ttctggtgag tcatcagatg
     6481 taggaatgta cctgaaaacc ttacttagta tactgtgtac actgtttaat ggccatgcat
     6541 cttttgaagt ttgatatttt ctgaaaaagc attgtttgcc tcagccctct tctttttacg
     6601 tatactccat ttatcattga tattttagat gtttcttatc aacaaaattg gctgccctgg
     6661 ttagtgctga gtcatcgcaa gacttgcttt tgcatttagg aagccacacc ctcccacaaa
     6721 acagaacagt gcaaaataag cagcagagag acagacagtt ggaaaagaca tgttttcttt
     6781 aggtattaat atcttaatta aatggaatca gattttgtat ggaaatgagt ttatattaca
     6841 tttccattat actaagtcag tgtgaaagtt taccatcaaa atagcgttac ttcttttatg
     6901 actaactctg atttgcttat acatatcaga atgatattca ttaaatattt taatttcatt
     6961 tatttttcta tgttttgatt agtttcacat caagtctgaa tactatattt tggaagaatc
     7021 caaaaccaca tatgaagata ctgattttaa aagtcaggat ttcatgtacc aagaatgacc
     7081 tacttgaagg agattttgct acactcttag agtcccaaaa attccccttt aaaaaaaatg
     7141 ctgtcctaca gaaggtgtat aaactatacc ttagccataa ttcaatttaa tgaaatacta
     7201 ccccattaaa agccatcctc ctgcctcata ccgattcaga aattaaattc tgaactttta
     7261 gtgtaggcat taggaatcac ttagccactt aataaggact gagattcttg ggtggcagaa
     7321 aaatcctggt gtgctagatt cttagtcact ttaaactgca tagaagtaat gagcataggc
     7381 ttcagtatat ttaataaggt gctgaggcag cagattcaaa tgcaaaagag ggaggaacca
     7441 gttattccaa ggcaggatga tgcaccccca cacagagtgt ggaaaaatta aagtgaaaca
     7501 aagatttgga cagtgtcatt aggaagcctg aggagaacat gctacaaggc cagatcctgt
     7561 cctatggaaa ataaaaaaga tcagcagaac taaagcaaaa gcagtcatct ccagtgtgtg
     7621 ggccttagca aagagtaaac caaatacctc tccttgtgtt aggagcactg ttatattccc
     7681 agctcagaga tgattgtgtg ctgggaaatg cttatattca ttatttccca actaatgcat
     7741 ttggacatat gtattccagt ttttcaaaga cactacccaa agcttcttta catttgcatt
     7801 ggttatattt aatatttctt aagctaagat cactgattat ccacacgtta ttagaaatct
     7861 ttggttacag tgaggcttaa cctgggctaa agtctcctaa atgggactac acttagatcc
     7921 gccctatttc acctttggac taacgttgac taaatggctc ctagagttac ccacagaggg
     7981 agcttactat gatgagagct actgtgggga aacatagtat tcaacagaga aatggaagaa
     8041 tacaagtagc aatgttcctt gtcttcaata acatttgagg gaaaggaatc tagaaatcct
     8101 aaagcttagt cagggtgcct aaggtaagac acaaaaagaa agagacatcc agaaaggaag
     8161 gttgaaagga aatgaagggc agtaacaaag caccaatgta aaatgaccca tagccaacaa
     8221 taaatgcaat aacaataaag gaaactaacc ctcacaaagg gtttgaggag cttcctgctg
     8281 tgtgtcctta aacagtgcgt gcctcttaag tctctacaac acttttttaa agggcattga
     8341 attggacatt ggtttaattt ttacctacgc tccctataat ctcaggaggt aaccattatt
     8401 taatcagaat tacccttgct ctgtttatct gtgaggcctc agacacaacg gtggttcagc
     8461 cctctcccta agcagtagca agctgagtaa tggcacacat atgtctagga tagccagcat
     8521 tcaggaaaat tgctgtgaac ctcctgtttt taattttaat atagaaaaag tctttcaggt
     8581 ttgtgacacg aagacagatt tagcttccca cttgtcagca aatttgtaaa aacctcaaaa
     8641 caaatgaata tttggaattc actctaagag atacaaatcc tgaatttcta actatcttct
     8701 caaatattac tgaggccagc aagatgcaag tgttctacaa aataatgaac tgttctaagt
     8761 gacagtgttg atgctggaag tggaatgtta tgttttcgct cagctgtatt cattcagaaa
     8821 gtttttcttg aacatctgcc acatgaggtg cagagaatac taccaggtgc tagtttttcc
     8881 agtatttgac ttctgattac tatttccttt tctcatcttt agtttttcaa gattttgctt
     8941 taccaaaata gtaaagcctt tatcatcagc ttatattgaa taatgttgta attggtttca
     9001 atcaaagttt ctcctcaggt acttgggggc ccctagcctt ctaaggaact cccaggcacc
     9061 tacttaacaa ggccagctac acactcagta tgtgataagc cccatgatgg atgcaggtta
     9121 gaattcaaag acctggttgg agtcctagat gtggagacag gatgatcagg tcacacttgt
     9181 tagatgacta acactatcag tagaagctct tgagagattt tcctaacgca gcaagatttc
     9241 tgtgagtaga ggtatcctgg gaggtatcct gggaggcagc ctattgactt gaccaagtaa
     9301 gctgatcagg tggcctcctc tacccactaa agaaatgtgt aaacactagc aataattgct
     9361 ttatcttaaa ctcctggaca tactcagttc ttccattcca ctgttctatt gccaatacct
     9421 tttgttgttt tcttcacact cctcttggca gcaaatgtct gaaagtattt caattgtgta
     9481 atgttaagga gttttttcat agcttcagaa aagagggcag caaatatgaa gccttaagtt
     9541 caaaataagt cattctacct agaaatacag accccagagc acattgcatg aaaatacctg
     9601 tactctgcag ttcctcaaag cagtattctt cctgaaaagc caaacaccac acctattttc
     9661 ctatttgcta agaatcagaa taagcacgtt gtaaatagta tccaaagcag attctaaaat
     9721 gacatagtaa gaagccagat tcaaattgta accaaagaag acaatagaaa tcccacttta
     9781 ccccactgtc atcagttaga acacccttgc aaaaactgta accacttaag caattcatct
     9841 gatcccagaa gatcatacct tcttttgaaa gtataggaca gatatcagtg ggaaacgtcg
     9901 gcgttctgag caacacagga taaatgtagg agggccttaa aaaataaatc tcaattcata
     9961 cactggagca gcaaaaaact gagcaggaaa ggaaacagaa tccaaagtca tttttcatat
    10021 agctgttgtc aaatagtata accttggtgt cttctttgag ttgcctggac agtatttatg
    10081 aaacaaaaaa ctaaatgccc cccatttggg gacgggggga ggggttcaga cctctaacct
    10141 ggattcagag ccttagaggc cgagagggaa tctggaatct ggtattactg agatcctagg
    10201 taaaagaacc agcctggcag tctttcccac ctcattggtc cgtgctttta tttttaaacc
    10261 caaaagaaag aaagaaacac accctcttat gtaggaattt cccttttaca aataatttga
    10321 cctggtagaa ataaacttgc ctgcctgctc ttaaatgcca gacagttgga agcaaatgcc
    10381 gagggaaagg tgcccagagc catgcttgat aggactttga atattttctc cttaattaaa
    10441 gtacgttgct tgtatttaga ctataagatg ctatggaggt ctatcccatg ccatgccaat
    10501 gtgaattgct ttgcttcagt aacaatcaga agaccagtcc aacagaaaat aacttgtcat
    10561 aattccacct tagattctag acctctcata cctgcagtgt acagaatatg tacatgttcc
    10621 aatggaattc actatttttg gctttagtgt caaagagatt ggttctacaa ggttcatctg
    10681 atttcccata acaagtaaat tttataatcc tatgattcta aattcaatcc ccaatataga
    10741 ttctaagcat caaatcaaaa tcacagacaa aggggaactg gtcgagaggg gtcttagtta
    10801 tttcaaatcc atgaccaaag tgtccaaaga catgaaactc ttatacctgc tgagcatttc
    10861 actttactat acaaaatgtc agctacccag ttgcatcctg tgacatgatc agactgtcaa
    10921 tgtggaccag tggccaggag catatttatg ggccatttct gttcatcatt ctttacagag
    10981 cattgaggtt tcccactgaa acagcttctt tagtcagacg tctatagatt ttacataaat
    11041 ttacatttaa atgcattaag ttagatggcc caattgagca tctgaatgaa tatagtgggg
    11101 gttggtggtg gtgcaaattc tgctggcttt atgttatggt tttcttcgtg ttttttcttg
    11161 gttttgtctg gcttcttctg gcaagtgccc taaaagactg gaacactgta taaagtcata
    11221 gacatagaac catatgggaa agcccagatg aaaaaatgga agaataaaat caagttgtca
    11281 aagttccagc aacagccctg acttcttcag gaatccaagc aaattgaaag ccaagacaaa
    11341 atgtacaaat ggtgcccatg ccattcattt gactgtgggt ggccctctag tctagggctc
    11401 tcttagtgaa tggttgtgga aatatgattt ttctaagttc cttccttttc cttttgatag
    11461 atgagtttga gatgatggag taggagtggg gccctcaggc acttctggta aagacattcc
    11521 acctgcaagc agcattttga gtaaagcact gctgtggttt gccgatttat ggtccattta
    11581 atgttaggct aaagcacctt taatcatttt tgttgtttta agataatgta tttgtgaagt
    11641 ggataaacac tggaaatagg gtgcttcttc tggaaagttc agtgtaaaac acaaacaagg
    11701 ctttggcggg tttatctggc tttataaaca agtctgaaaa atggatgaaa gctaaatata
    11761 taaagcagtt ggttgtctat cttttatcat ttttactcag atctgtattt aacacttatt
    11821 tatttgttag tttttacatt caaaagaaat acactttgaa ctttggctaa cattgtagga
    11881 tattttttaa ttgtttctac atttttaaag catgattcat catttttgta aacttagatc
    11941 attttttaat tgtcttttct tttccaatag accagttacc actcatgtgt ctgcagaacc
    12001 tctttattgt attcctataa taaatgtaaa atatttgtag caaa
//
