LOCUS       NM_004111               2016 bp    mRNA    linear   PRI 13-MAY-2024
DEFINITION  Homo sapiens flap structure-specific endonuclease 1 (FEN1), mRNA.
ACCESSION   NM_004111
VERSION     NM_004111.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Wu,M., Huang,X., Wu,B., Zhu,M., Zhu,Y., Yu,L., Lan,T. and Liu,J.
  TITLE     The endonuclease FEN1 mediates activation of STAT3 and facilitates
            proliferation and metastasis in breast cancer
  JOURNAL   Mol Biol Rep 51 (1), 553 (2024)
   PUBMED   38642158
  REMARK    GeneRIF: The endonuclease FEN1 mediates activation of STAT3 and
            facilitates proliferation and metastasis in breast cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Berfelde,J., Hildebrand,L.S., Kuhlmann,L., Fietkau,R. and
            Distel,L.V.
  TITLE     FEN1 Inhibition as a Potential Novel Targeted Therapy against
            Breast Cancer and the Prognostic Relevance of FEN1
  JOURNAL   Int J Mol Sci 25 (4), 2110 (2024)
   PUBMED   38396787
  REMARK    GeneRIF: FEN1 Inhibition as a Potential Novel Targeted Therapy
            against Breast Cancer and the Prognostic Relevance of FEN1.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2016)
  AUTHORS   Wang,S., Wang,X., Sun,J., Yang,J., Wu,D., Wu,F. and Zhou,H.
  TITLE     Down-regulation of DNA key protein-FEN1 inhibits OSCC growth by
            affecting immunosuppressive phenotypes via IFN-gamma/JAK/STAT-1
  JOURNAL   Int J Oral Sci 15 (1), 17 (2023)
   PUBMED   37185662
  REMARK    GeneRIF: Down-regulation of DNA key protein-FEN1 inhibits OSCC
            growth by affecting immunosuppressive phenotypes via
            IFN-gamma/JAK/STAT-1.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2016)
  AUTHORS   Xiao,Y., Yin,M., Wang,Y., Shi,R., Mao,S., Hua,Y. and Xu,H.
  TITLE     Post-translational Modifications by Acyl Groups Regulate FEN1's
            Activities and Play Essential Roles in Cell Proliferation and DNA
            Repair
  JOURNAL   Protein Pept Lett 30 (7), 597-607 (2023)
   PUBMED   37254539
  REMARK    GeneRIF: Post-translational Modifications by Acyl Groups Regulate
            FEN1's Activities and Play Essential Roles in Cell Proliferation
            and DNA Repair.
REFERENCE   5  (bases 1 to 2016)
  AUTHORS   Laverde,E.E., Polyzos,A.A., Tsegay,P.P., Shaver,M., Hutcheson,J.D.,
            Balakrishnan,L., McMurray,C.T. and Liu,Y.
  TITLE     Flap Endonuclease 1 Endonucleolytically Processes RNA to Resolve
            R-Loops through DNA Base Excision Repair
  JOURNAL   Genes (Basel) 14 (1), 98 (2022)
   PUBMED   36672839
  REMARK    GeneRIF: Flap Endonuclease 1 Endonucleolytically Processes RNA to
            Resolve R-Loops through DNA Base Excision Repair.
            GeneRIF: Flap Endonuclease 1 Endonucleolytically Processes RNA to
            Resolve R-Loops through DNA Base Excision Repair.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2016)
  AUTHORS   Li,X., Li,J., Harrington,J., Lieber,M.R. and Burgers,P.M.
  TITLE     Lagging strand DNA synthesis at the eukaryotic replication fork
            involves binding and stimulation of FEN-1 by proliferating cell
            nuclear antigen
  JOURNAL   J Biol Chem 270 (38), 22109-22112 (1995)
   PUBMED   7673186
REFERENCE   7  (bases 1 to 2016)
  AUTHORS   Hiraoka,L.R., Harrington,J.J., Gerhard,D.S., Lieber,M.R. and
            Hsieh,C.L.
  TITLE     Sequence of human FEN-1, a structure-specific endonuclease, and
            chromosomal localization of the gene (FEN1) in mouse and human
  JOURNAL   Genomics 25 (1), 220-225 (1995)
   PUBMED   7774922
REFERENCE   8  (bases 1 to 2016)
  AUTHORS   Robins,P., Pappin,D.J., Wood,R.D. and Lindahl,T.
  TITLE     Structural and functional homology between mammalian DNase IV and
            the 5'-nuclease domain of Escherichia coli DNA polymerase I
  JOURNAL   J Biol Chem 269 (46), 28535-28538 (1994)
   PUBMED   7961795
REFERENCE   9  (bases 1 to 2016)
  AUTHORS   Murray,J.M., Tavassoli,M., al-Harithy,R., Sheldrick,K.S.,
            Lehmann,A.R., Carr,A.M. and Watts,F.Z.
  TITLE     Structural and functional conservation of the human homolog of the
            Schizosaccharomyces pombe rad2 gene, which is required for
            chromosome segregation and recovery from DNA damage
  JOURNAL   Mol Cell Biol 14 (7), 4878-4888 (1994)
   PUBMED   8007985
REFERENCE   10 (bases 1 to 2016)
  AUTHORS   Kemeny,M.M., Alava,G. and Oliver,J.M.
  TITLE     Improving responses in hepatomas with circadian-patterned hepatic
            artery infusions of recombinant interleukin-2
  JOURNAL   J Immunother (1991) 12 (4), 219-223 (1992)
   PUBMED   1477073
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP244711.1 and BC000323.2.
            
            On Nov 23, 2018 this sequence version replaced NM_004111.5.
            
            Summary: The protein encoded by this gene removes 5' overhanging
            flaps in DNA repair and processes the 5' ends of Okazaki fragments
            in lagging strand DNA synthesis. Direct physical interaction
            between this protein and AP endonuclease 1 during long-patch base
            excision repair provides coordinated loading of the proteins onto
            the substrate, thus passing the substrate from one enzyme to
            another. The protein is a member of the XPG/RAD2 endonuclease
            family and is one of ten proteins essential for cell-free DNA
            replication. DNA secondary structure can inhibit flap processing at
            certain trinucleotide repeats in a length-dependent manner by
            concealing the 5' end of the flap that is necessary for both
            binding and cleavage by the protein encoded by this gene.
            Therefore, secondary structure can deter the protective function of
            this protein, leading to site-specific trinucleotide expansions.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163658.424764.1,
                                           SRR1660805.214356.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000305885.3/ ENSP00000305480.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-339               BP244711.1         255-593
            340-2016            BC000323.2         330-2006
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q12.2"
     gene            1..2016
                     /gene="FEN1"
                     /gene_synonym="FEN-1; MF1; RAD2"
                     /note="flap structure-specific endonuclease 1"
                     /db_xref="GeneID:2237"
                     /db_xref="HGNC:HGNC:3650"
                     /db_xref="MIM:600393"
     exon            1..118
                     /gene="FEN1"
                     /gene_synonym="FEN-1; MF1; RAD2"
                     /inference="alignment:Splign:2.1.0"
     exon            119..2016
                     /gene="FEN1"
                     /gene_synonym="FEN-1; MF1; RAD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             140..1282
                     /gene="FEN1"
                     /gene_synonym="FEN-1; MF1; RAD2"
                     /EC_number="3.1.26.4"
                     /note="DNase IV; maturation factor-1"
                     /codon_start=1
                     /product="flap endonuclease 1"
                     /protein_id="NP_004102.1"
                     /db_xref="CCDS:CCDS8010.1"
                     /db_xref="GeneID:2237"
                     /db_xref="HGNC:HGNC:3650"
                     /db_xref="MIM:600393"
                     /translation="MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLI
                     AVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSE
                     RRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAE
                     ASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGL
                     NQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLH
                     KEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQG
                     STQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK"
     regulatory      1998..2003
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FEN1"
                     /gene_synonym="FEN-1; MF1; RAD2"
                     /note="hexamer: AATAAA"
     polyA_site      2016
                     /gene="FEN1"
                     /gene_synonym="FEN-1; MF1; RAD2"
                     /note="major polyA site"
ORIGIN      
        1 ggctgaacgt caggccaccc gccgctaagc tgagaaggga gagcgagctt aggaccgcct
       61 gcccggggca accccgaacc aagctttagc cgccgaggcc gcgtgtccca aaggccagtc
      121 atccctcctc tgtgttgcca tgggaattca aggcctggcc aaactaattg ctgatgtggc
      181 ccccagtgcc atccgggaga atgacatcaa gagctacttt ggccgtaagg tggccattga
      241 tgcctctatg agcatttatc agttcctgat tgctgttcgc cagggtgggg atgtgctgca
      301 gaatgaggag ggtgagacca ccagccacct gatgggcatg ttctaccgca ccattcgcat
      361 gatggagaac ggcatcaagc ccgtgtatgt ctttgatggc aagccgccac agctcaagtc
      421 aggcgagctg gccaaacgca gtgagcggcg ggctgaggca gagaagcagc tgcagcaggc
      481 tcaggctgct ggggccgagc aggaggtgga aaaattcact aagcggctgg tgaaggtcac
      541 taagcagcac aatgatgagt gcaaacatct gctgagcctc atgggcatcc cttatcttga
      601 tgcacccagt gaggcagagg ccagctgtgc tgccctggtg aaggctggca aagtctatgc
      661 tgcggctacc gaggacatgg actgcctcac cttcggcagc cctgtgctaa tgcgacacct
      721 gactgccagt gaagccaaaa agctgccaat ccaggaattc cacctgagcc ggattctgca
      781 ggagctgggc ctgaaccagg aacagtttgt ggatctgtgc atcctgctag gcagtgacta
      841 ctgtgagagt atccggggta ttgggcccaa gcgggctgtg gacctcatcc agaagcacaa
      901 gagcatcgag gagatcgtgc ggcgacttga ccccaacaag taccctgtgc cagaaaattg
      961 gctccacaag gaggctcacc agctcttctt ggaacctgag gtgctggacc cagagtctgt
     1021 ggagctgaag tggagcgagc caaatgaaga agagctgatc aagttcatgt gtggtgaaaa
     1081 gcagttctct gaggagcgaa tccgcagtgg ggtcaagagg ctgagtaaga gccgccaagg
     1141 cagcacccag ggccgcctgg atgatttctt caaggtgacc ggctcactct cttcagctaa
     1201 gcgcaaggag ccagaaccca agggatccac taagaagaag gcaaagactg gggcagcagg
     1261 gaagtttaaa aggggaaaat aaatgtgttt ccccattata cctccttcac cccagaatat
     1321 ttgccgtctt gtacccttaa gagctacagc tagagaaacc ttcacggggt ggagagagga
     1381 ttctaaggct tttctagcgt gacccttttc agtagtgcta gtcccttttt tacttgatct
     1441 taatggcaag aaggccacag aggtactttt ccttttttag ctcaggaaaa tatgtcaggc
     1501 tcaaaccact tctcaggcag tttaatggac actaagtcca ttgttacatg aaagtgatag
     1561 atagcaacaa gttttggaga agagagaggg agataaaagg gggagacaaa agatgtacag
     1621 aaatgatttc ctggctggcc aactggtggc cagtgggagg tgatggtgga cctagactgt
     1681 gcttttctgt cttgttcagc cttgacccac cttgagagag agccaccagg aaggcgcatc
     1741 ttagcagatg ggaggaactg ctgagagaag atgggcagaa agctggagcc cctggagttg
     1801 gctgtgtctg tgtttgtgac tgattactgg ctgtgtcttg ggtgggcaga aactcgaact
     1861 tgctatgtaa tttgtgtcta gttattcaga ggagtaagat ggtgatgttc acctggcaat
     1921 cagctgagtt gagactttgg aataagacac tggttttcat gcgctgtttt tgttttaaag
     1981 ttatgaagaa aaaagtcaat aaaattctaa aagtaa
//
