LOCUS       NM_000140               7689 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens ferrochelatase (FECH), transcript variant 2, mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_000140
VERSION     NM_000140.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Zhao,Y., Zhu,L., Shi,D., Gao,J. and Fan,M.
  TITLE     Key Genes FECH and ALAS2 under Acute High-Altitude Exposure: A Gene
            Expression and Network Analysis Based on Expression Profile Data
  JOURNAL   Genes (Basel) 15 (8), 1075 (2024)
   PUBMED   39202434
  REMARK    GeneRIF: Key Genes FECH and ALAS2 under Acute High-Altitude
            Exposure: A Gene Expression and Network Analysis Based on
            Expression Profile Data.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 7689)
  AUTHORS   Lanzafame,M., Branca,G., Landi,C., Qiang,M., Vaz,B., Nardo,T.,
            Ferri,D., Mura,M., Iben,S., Stefanini,M., Peverali,F.A., Bini,L.
            and Orioli,D.
  TITLE     Cockayne syndrome group A and ferrochelatase finely tune ribosomal
            gene transcription and its response to UV irradiation
  JOURNAL   Nucleic Acids Res 49 (19), 10911-10930 (2021)
   PUBMED   34581821
  REMARK    GeneRIF: Cockayne syndrome group A and ferrochelatase finely tune
            ribosomal gene transcription and its response to UV irradiation.
REFERENCE   4  (bases 1 to 7689)
  AUTHORS   Khayer,N., Jalessi,M., Jahanbakhshi,A., Tabib Khooei,A. and
            Mirzaie,M.
  TITLE     Nkx3-1 and Fech genes might be switch genes involved in pituitary
            non-functioning adenoma invasiveness
  JOURNAL   Sci Rep 11 (1), 20943 (2021)
   PUBMED   34686726
  REMARK    GeneRIF: Nkx3-1 and Fech genes might be switch genes involved in
            pituitary non-functioning adenoma invasiveness.
            Erratum:[Sci Rep. 2021 Dec 14;11(1):24258. doi:
            10.1038/s41598-021-03094-1.. PMID: 34907222]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7689)
  AUTHORS   Balwani,M., Bloomer,J. and Desnick,R.
  CONSRTM   Porphyrias Consortium of the NIH-Sponsored Rare Diseases Clinical
            Research Network
  TITLE     Erythropoietic Protoporphyria, Autosomal Recessive
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   23016163
REFERENCE   6  (bases 1 to 7689)
  AUTHORS   Brenner,D.A., Didier,J.M., Frasier,F., Christensen,S.R., Evans,G.A.
            and Dailey,H.A.
  TITLE     A molecular defect in human protoporphyria
  JOURNAL   Am J Hum Genet 50 (6), 1203-1210 (1992)
   PUBMED   1376018
REFERENCE   7  (bases 1 to 7689)
  AUTHORS   Nakahashi,Y., Fujita,H., Taketani,S., Ishida,N., Kappas,A. and
            Sassa,S.
  TITLE     The molecular defect of ferrochelatase in a patient with
            erythropoietic protoporphyria
  JOURNAL   Proc Natl Acad Sci U S A 89 (1), 281-285 (1992)
   PUBMED   1729699
REFERENCE   8  (bases 1 to 7689)
  AUTHORS   Nakahashi,Y., Taketani,S., Okuda,M., Inoue,K. and Tokunaga,R.
  TITLE     Molecular cloning and sequence analysis of cDNA encoding human
            ferrochelatase
  JOURNAL   Biochem Biophys Res Commun 173 (2), 748-755 (1990)
   PUBMED   2260980
REFERENCE   9  (bases 1 to 7689)
  AUTHORS   Buller,R.E., Schrader,W.T. and O'Maller,B.W.
  TITLE     Steroids and the practical aspects of performing binding studies
  JOURNAL   J Steroid Biochem 7 (5), 321-326 (1976)
   PUBMED   180343
REFERENCE   10 (bases 1 to 7689)
  AUTHORS   Bonkowsky,H.L., Bloomer,J.R., Ebert,P.S. and Mahoney,M.J.
  TITLE     Heme synthetase deficiency in human protoporphyria. Demonstration
            of the defect in liver and cultured skin fibroblasts
  JOURNAL   J Clin Invest 56 (5), 1139-1148 (1975)
   PUBMED   1184741
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC100847.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Oct 15, 2019 this sequence version replaced NM_000140.4.
            
            Summary: The protein encoded by this gene is localized to the
            mitochondrion, where it catalyzes the insertion of the ferrous form
            of iron into protoporphyrin IX in the heme synthesis pathway.
            Mutations in this gene are associated with erythropoietic
            protoporphyria. Two transcript variants encoding different isoforms
            have been found for this gene. A pseudogene of this gene is found
            on chromosome 3.[provided by RefSeq, May 2010].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX571744.1, BC039841.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000262093.11/
                                                  ENSP00000262093.6
            RefSeq Select criteria             :: based on manual assertion,
                                                  conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-149               AC100847.2         42812-42960
            150-276             AC100847.2         49315-49441
            277-396             AC100847.2         56149-56268
            397-545             AC100847.2         57974-58122
            546-680             AC100847.2         62933-63067
            681-787             AC100847.2         66534-66640
            788-886             AC100847.2         70271-70369
            887-994             AC100847.2         74562-74669
            995-1159            AC100847.2         75090-75254
            1160-1219           AC100847.2         78140-78199
            1220-7689           AC100847.2         78668-85137
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.31"
     gene            1..7689
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /note="ferrochelatase"
                     /db_xref="GeneID:2235"
                     /db_xref="HGNC:HGNC:3647"
                     /db_xref="MIM:612386"
     exon            1..149
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /inference="alignment:Splign:2.1.0"
     CDS             83..1354
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /EC_number="4.98.1.1"
                     /note="isoform b precursor is encoded by transcript
                     variant 2; protoporphyria; protoheme ferro-lyase; heme
                     synthetase; ferrochelatase, mitochondrial; heme synthase"
                     /codon_start=1
                     /product="ferrochelatase, mitochondrial isoform b
                     precursor"
                     /protein_id="NP_000131.2"
                     /db_xref="CCDS:CCDS11964.1"
                     /db_xref="GeneID:2235"
                     /db_xref="HGNC:HGNC:3647"
                     /db_xref="MIM:612386"
                     /translation="MRSLGANMAAALRAAGVLLRDPLASSSWRVCQPWRWKSGAAAAA
                     VTTETAQHAQGAKPQVQPQKRKPKTGILMLNMGGPETLGDVHDFLLRLFLDRDLMTLP
                     IQNKLAPFIAKRRTPKIQEQYRRIGGGSPIKIWTSKQGEGMVKLLDELSPNTAPHKYY
                     IGFRYVHPLTEEAIEEMERDGLERAIAFTQYPQYSCSTTGSSLNAIYRYYNQVGRKPT
                     MKWSTIDRWPTHHLLIQCFADHILKELDHFPLEKRSEVVILFSAHSLPMSVVNRGDPY
                     PQEVSATVQKVMERLEYCNPYRLVWQSKVGPMPWLGPQTDESIKGLCERGRKNILLVP
                     IAFTSDHIETLYELDIEYSQVLAKECGVENIRRAESLNGNPLFSKALADLVHSHIQSN
                     ELCSKQLTLSCPLCVNPVCRETKSFFTSQQL"
     exon            150..276
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /inference="alignment:Splign:2.1.0"
     exon            277..396
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /inference="alignment:Splign:2.1.0"
     exon            397..545
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /inference="alignment:Splign:2.1.0"
     exon            546..680
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /inference="alignment:Splign:2.1.0"
     exon            681..787
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /inference="alignment:Splign:2.1.0"
     exon            788..886
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /inference="alignment:Splign:2.1.0"
     exon            887..994
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1159
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1219
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..7689
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2531..2536
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /note="hexamer: ATTAAA"
     polyA_site      2558
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /note="major polyA site"
     regulatory      2708..2713
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /note="hexamer: AATAAA"
     polyA_site      2726
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
     regulatory      3738..3743
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /note="hexamer: AATAAA"
     polyA_site      3758
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
     regulatory      6796..6801
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /note="hexamer: ATTAAA"
     polyA_site      6818
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
     regulatory      7202..7207
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /note="hexamer: AACAAA"
     polyA_site      7221
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
     regulatory      7656..7661
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
                     /note="hexamer: AGTAAA"
     polyA_site      7689
                     /gene="FECH"
                     /gene_synonym="EPP; EPP1; FCE"
ORIGIN      
        1 acccggctcg gccactgctg ggcggacacc tgggcgcgcc gccgcgggag gagcccggac
       61 tcgggccgag gctgcccagg caatgcgttc actcggcgca aacatggctg cggccctgcg
      121 cgccgcgggc gtcctgctcc gcgatccgct ggcatccagc agctggaggg tctgtcagcc
      181 atggaggtgg aagtcaggtg cagctgcagc ggccgtcacc acagaaacag cccagcatgc
      241 ccagggtgca aaacctcaag ttcaaccgca gaagaggaag ccgaaaactg gaatattaat
      301 gctaaacatg ggaggccctg aaactcttgg agatgttcac gacttccttc tgagactctt
      361 cttggaccga gacctcatga cacttcctat tcagaataag ctggcaccat tcatcgccaa
      421 acgccgaacc cccaagattc aagagcagta ccgcaggatt ggaggcggat cccccatcaa
      481 gatatggact tccaagcagg gagagggcat ggtgaagctg ctggatgaat tgtcccccaa
      541 cacagcccct cacaaatact atattggatt tcggtacgtc catcctttaa cagaagaagc
      601 aattgaagag atggagagag atggcctaga aagggctatt gctttcacac agtatccaca
      661 gtacagctgc tccaccacag gcagcagctt aaatgccatt tacagatact ataatcaagt
      721 gggacggaag cccacgatga agtggagcac tattgacagg tggcccacac atcacctcct
      781 catccagtgc tttgcagatc atattctaaa ggaactggac cattttccac ttgagaagag
      841 aagcgaggtg gtcattctgt tttctgctca ctcactgccc atgtctgtgg tcaacagagg
      901 cgacccatat cctcaggagg taagcgccac tgtccaaaaa gtcatggaaa ggctggagta
      961 ctgcaacccc taccgactgg tgtggcaatc caaggttggt ccaatgccct ggttgggtcc
     1021 tcaaacagac gaatctatca aagggctttg tgagaggggg aggaagaata tcctcttggt
     1081 tccgatagca tttaccagtg accatattga aacgctgtat gagctggaca tcgagtactc
     1141 tcaagtttta gccaaggagt gtggagttga aaacatcaga agagctgagt ctcttaatgg
     1201 aaatccattg ttctctaagg ccctggccga cttggtgcat tcacacatcc agtcaaacga
     1261 gctgtgttcc aagcagctga ccctgagctg tccgctctgt gtcaatcctg tctgcaggga
     1321 gactaaatcc ttcttcacca gccagcagct gtgacccccg ccggtggacc ccgtggcgtt
     1381 aggcaaatgc ccaacctcca gatacctccg atgtggagag ggtgttattt agagatcaag
     1441 gaaggaagtc atccttcctt gatatatata cagcctttgg gtacaaattg tgtggtttct
     1501 tgaggattgg actcttgatg gatttctatt tttatataac tatacagtaa gcatttgtat
     1561 tttctctctc taggtataag ttactagttt ggaatgtcca tcaggacctt taataaatga
     1621 gttaaaaatt tgtcttatga gacacaccta tttaagtaca gattttggct ttattgccca
     1681 aaaccctcct gaaagggtac ggagagtccc ctctgtgggc tggcagtgtg aatgagatct
     1741 gtttagtctc gtgcatatag ttgctgtttt ttaaatgaac acagttgagt atttgaagtg
     1801 aatttgaaaa agaaatgtta cttaatcttt ccctaagccc atgggttaca gaatgctagg
     1861 gaggcaattt ggttacctgc aatggctgct tttgccagcg aggccaccat tcattggtca
     1921 tcttggtatt tgtgtgtgaa tctcactttc ctcaatgtaa aaaggaatca agtatggatt
     1981 tcagaggtgc tcttagattc cccatacacc caagggtaat aaacgtgtac aagtacagtg
     2041 ttcatgatac gtgccttggt gggagtccgt ggtgccacag ggaaggggct cccactgctt
     2101 ctggtctcca gggacagtgc tgctggaaag gctagtgatg agcttcaccc tggagctcct
     2161 cccgggacct tgcaagcctc tccatccagc atcttctcta tcttagttga atgccttctt
     2221 tctgaacatt tgttttaaga attattttat aaagtcaaca atactttgct tgaattcttt
     2281 cttaatttac gattttttat tataaaaatg tatagtgata caatgggaca tgtgaagaat
     2341 acagaaaagt aaccacttta atgcaataac tgttatcata atattgtatt tcgtggtagt
     2401 cctttcctgt agatattttt aatgccattt aatgccattg tcaccttgga tttatgagtg
     2461 aaaagtgttt ctaaaaatat agaaataatg tcagatcaga gtctgatctt ctatgtttgt
     2521 atttaaatgg attaaaagat ccccggtggt tccatgaaga atttgtaaag atcactttct
     2581 ctttcctcca agccctgaaa ctttgttctt caaaagagcg tttctttttt tttttttttt
     2641 tagccagttt ataaagtgga agtattagga gattcataaa tcttctatat tgagaattgg
     2701 ctatgttaat aaatattaca acatcattaa ggttttagct aagtttgatt catgctgtct
     2761 gttaaatcaa aactgatcta aatcagaatt attaaatgtg aggagctttt ttaatacagg
     2821 aaaagaaaca tgtcatccac ttgagttaat agttttccta cgttgatgac agccctcatg
     2881 agtagcatcc acatttttaa aatttcaaat tggtttttct actagtagat tgtgtttcta
     2941 gagaaagata caaggcatag gtgattgttt aggattttcc tctagccttt gccattacct
     3001 ttttggggat gaggttcaca gtagactttg agtgaccgtc ccaccgtgaa gtgaattctc
     3061 tgagctggtg gtgtggtgct ggaaggaagg ttatttttgg agccactctc tccccttaag
     3121 gatatttccc aagggcctgc ttcaattctt tgatgacttt agaggtgaaa aaatattttt
     3181 atggagatga tgcagaaaac tccaattcag gagcccttgc gagtatatct gaagcactta
     3241 tttgctaagg aaacctgaat tgatagcagt actgtgctgt ctggaataat gtccttgata
     3301 ctgagttggg accagactgg cttttatagt gacaggcaaa gaggaattta ttgagatcac
     3361 tgctcatggc atttgttgct gtaagaagtg ttgcctttga ttgttactaa ccacggatgg
     3421 gtaacggtca tacattaggc tagtgtttgg taggacaaaa tctttttaga gctttgagaa
     3481 ttgtcatcct gttggtcaac tttgaaatac aaatgtttgc cctggtaatt agcaatgaac
     3541 tgctggcagt ttcttcagct gtgtatatac ggatctggct tttaattgat gaatcaactt
     3601 ctacagaaac ttttgcaggg acagtgttga tgaggcagtt tagcttgcca gggtgatgat
     3661 aaagcccagg tccctgcatg tatagtgctc ttctaaagaa tatgcattct tgaactactt
     3721 aactttttaa aaatcacaat aaatttttgc actcaaaatt tgcttcgtat caggagaaat
     3781 gaactcattg ttttgttttg tttttttttt tttttaagat ggagtcttgc tatgtcaccc
     3841 aggctggagg gcagtggtgc gatctcggct cactgctact tccacctcct gggctcaagt
     3901 gatcctccca cctcagcctc caagtagctg ggactacagg agtgcttcac cacgctgggc
     3961 tactttttta tattttttgt agagatgggg ttttgccatg ttgtccaggc tggtcttgaa
     4021 ctcctgggct caagggattc tcctgcctca gtctcccaaa gtgctgggat tacaaggatg
     4081 agcctctgca cctggccctg aactcattat taaaagccct ttaaatgtga ggctgggtgc
     4141 cgtgccttac atgtgtaatt ccaatacttt ggaaggccaa ggttggagga ttgcttgatc
     4201 ccaagagttc aagaccagcc tgggcaacat agggagaccc tgactctaca aaaaataaag
     4261 taaaaattaa ctgggtgtag tgtcacatgc ctgtagttcc agctacttag gaggctgagg
     4321 tggtaggatt gcttgagccc agcagtttga ggttgcagtg aggtgtgatt gcaccactgc
     4381 actccagcct gggtgacaga ggaagaccct gtcccaaaac caaaaaaaag aaaagaaata
     4441 cagagactgg gtcatttaca aaggaaagag gtttaattga ctcggttcgg ctttctgagg
     4501 aagccttagg aaattgacaa tcatggcaga aggggaagca gatgtcttac atggcagtga
     4561 gtgagagcaa gcaaagggga agagccccct tataaaacca tcagatctcg tgagaactgg
     4621 ctgtcacaag aacagcatgg gggaactgtc tccatgttcc aatctccttc caccaggtcc
     4681 ctccctcaac acgtggggat tatggggatt acaatttgaa atgagatttg ggtggggaac
     4741 agagccaaat catatcattc caccctggcc cctcccaaat cacatgtcct ttttacattt
     4801 caaaaccaat catgccttca caacagtcct ccagagtctt aactcattcc agcattaacc
     4861 caaaagtcca agttcaaagt ctcatccaag acaaggcaag tcccttctgc ctgtgagcct
     4921 gtaacattaa aagcaagtta gtgacttcca agatacaatg ggagtacaga cattggtaaa
     4981 tgttcccatt ccaaatggga gaaattggcc aaaacacagg ggctacaggc cccatgcacc
     5041 actgcactcc actgtgcaag tctgaaaccc ggcagggcac tccttaaatt tttttttttt
     5101 ttttttgaga tggagtctcg ctctgttgcc caagctggag tacagtggca cgatctcggc
     5161 tcactgcaac ctccgcctct tgggttcaaa ggatcatcct gcctcagcct ccggagtagc
     5221 tgggctactc aggcgtgtgc caccatgccc ggctaatttt tgtattttta gtagagatgg
     5281 ggcctgacca tgttggtcag gctggtctct aattcctgac ctcgtgatcc acccgcctca
     5341 gcctctgaaa gtgttgggat tacaggcgtg agccaccatc cccggcctac tcaataaatc
     5401 ttaaagttcc ggaataatct cctttgactc catgtctcac ctccaggtca cgctgatgca
     5461 agaggtgggc taatctttct agtaaattcc atatttaatt caagaaacca taacttaagg
     5521 catgtaaaag agatcctttg ctcaatgtga tgccattgtg cttatccaaa gtatattatt
     5581 attacccaca aagggtgaga gattaggctg cagccatacc ccaagtggag tgagcagcaa
     5641 gacctgcccc ctgctcagag tgtagatgac tgggggcacc tgcattccta ggggctctgc
     5701 cgtatgagct cctgtcgatg cggcaaagga ccaccttgcc caacgacagc gggaaggcag
     5761 aatttaaagc tggcagctgt aagcgaacgt ctatgtgtgc gcacgggggc acgtgaaggc
     5821 acaggtgcat cagccaagaa cctccaattc acctcttaac cttctcacct cacctgaaac
     5881 cccttctgcc agaatcctga aggtggccca ggaacagggc tcctaacgtt aggtggaaat
     5941 gggaaattca ttgagatgtc acaagctgga ataagaaaat tctgagctca cccggaaact
     6001 aatgccctaa attaagatta ttcagcttct caatttttaa tagcaaaatg gagacctgag
     6061 tgtggataac ttttagtatc tgtgggggat cctggaacca attccctgcc aatatagaag
     6121 gacaactgtc tacagtactt gaagtattat taactacatt cgccatgctg tatgttagat
     6181 ccccagaaca tatttatcct gcatatctaa aattttgatc attttacaaa ctttctattt
     6241 tttttgtcaa ttttctccag ctagacactt gtgcaatacg gctattatct gatctttgcc
     6301 ttaaatgttg tgcttctttt ccatatgcac gtattttgca aaatataaag tgtgtagagc
     6361 tatatagcac tcagccaagt ggtgggtacc tgcaggtgct tcagagaagt aaattgatgc
     6421 tgctaatatt tgttgaatgg cacgaatatg atgagcaata gcaggtggtg cccttcagcc
     6481 agaccatcgc tccgtgcgtc tgatgcatct tgccaaagag tagttctggg aggtggttgc
     6541 ctctagagaa cacattcctc ctattctggg gtcccgtgag agaaagaaat gcttttgctt
     6601 ttgatgtggg actcttacta agcctttctt cagagaaaag gaagtgaaaa atgcacccca
     6661 tgataatcag tttcttacaa catactgtga tagtaccggc ttcgttgttt ttagctggaa
     6721 tcattagctt ccatttttag aataacagct attggctaaa ttaggctaca gtaggccatt
     6781 aagatggatg ttggaattaa aaacattttt ggaaaaaagc ctgctttgag cctttgttat
     6841 aagcccttgg gtagagatct gggtcctgtt tctgatttct tgtgagcctt cactctgaca
     6901 gttttgtttc cagaaacaca ctcttagcct gctcctgaaa tgggaacaga caggccaact
     6961 tcccctctcc agtctcccct gcgggtcaaa gctttacttt cctgtcatgt taagaaagaa
     7021 tagatttaac cttgataatc catgtagtat tctgtatttt taccttttcc ttatctgaaa
     7081 aaaagtgtat atatggcatg gaattgattg cacaggcaca tggcatgttg gcttgtgaac
     7141 caattgttaa aatttcaagt taatcattaa aataatatct ttcaaattaa gttatattaa
     7201 aaacaaaggt aacattctaa attcattact ttctaattat ttcgcccttt tttttgtcgt
     7261 tgattattta gacttaagaa agtgatggga aaaacgttaa taacacagta cataagttgt
     7321 ttctgtagcc attacactgt gattttcaca aacacttgaa cacttgaata gtcttccatt
     7381 atttgaaaac tattattcaa cttatccaag tgaagtattg gcatacaaag aagttgtttc
     7441 atatttgcat tgctcatgaa tgtaaacaaa atgtcgatat gtgtgaacta tacttgttga
     7501 tcaattgcaa ccataggttg gctgcagatg cgagagcttg gtaaacatca acaaaagcat
     7561 tctctgagaa tgaattgact atatggaatt tgtaggaaag tgtattgtgt attttattat
     7621 tatttgtaaa ttgtgtattc cacatctttt agattagtaa aatttatagt aaacatactc
     7681 tcacaccta
//
