LOCUS       NM_001349798            5639 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens F-box and WD repeat domain containing 7 (FBXW7),
            transcript variant 5, mRNA.
ACCESSION   NM_001349798 XM_011532083
VERSION     NM_001349798.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Chen,Y., Chen,B., Tu,S. and Yuan,H.
  TITLE     miR-25-3p serves as an oncogenic in colorectal cancer cells by
            regulating the ubiquitin ligase FBXW7 function
  JOURNAL   Oncol Rep 52 (5) (2024)
   PUBMED   39329268
  REMARK    GeneRIF: miR-25-3p serves as an oncogenic in colorectal cancer
            cells by regulating the ubiquitin ligase FBXW7 function.
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Zhou,H., Jiang,Y., Zhou,Y., Zhang,Z. and Li,S.
  TITLE     miR-182-5p promotes the proliferation and invasion of hilar
            cholangiocarcinoma cells by inhibiting FBXW7
  JOURNAL   J Cancer Res Clin Oncol 150 (10), 461 (2024)
   PUBMED   39402299
  REMARK    GeneRIF: miR-182-5p promotes the proliferation and invasion of
            hilar cholangiocarcinoma cells by inhibiting FBXW7.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5639)
  AUTHORS   Li,J., Tian,J., Ma,M., Qin,Z., Cao,B., Yang,J., Wang,X. and Yang,X.
  TITLE     FAM83D promotes the progression of 4NQO-induced esophageal
            carcinoma via inhibiting FBWX7
  JOURNAL   Exp Cell Res 442 (2), 114252 (2024)
   PUBMED   39260674
  REMARK    GeneRIF: FAM83D promotes the progression of 4NQO-induced esophageal
            carcinoma via inhibiting FBWX7.
REFERENCE   4  (bases 1 to 5639)
  AUTHORS   Ohya,S., Kito,H., Kajikuri,J., Yamaguchi,Y. and Matsui,M.
  TITLE     Transcriptional Up-Regulation of FBXW7 by KCa1.1 K+ Channel
            Inhibition through the Nrf2 Signaling Pathway in Human Prostate
            Cancer LNCaP Cell Spheroid Model
  JOURNAL   Int J Mol Sci 25 (11), 6019 (2024)
   PUBMED   38892210
  REMARK    GeneRIF: Transcriptional Up-Regulation of FBXW7 by KCa1.1 K[+]
            Channel Inhibition through the Nrf2 Signaling Pathway in Human
            Prostate Cancer LNCaP Cell Spheroid Model.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5639)
  AUTHORS   Jin,J., Cardozo,T., Lovering,R.C., Elledge,S.J., Pagano,M. and
            Harper,J.W.
  TITLE     Systematic analysis and nomenclature of mammalian F-box proteins
  JOURNAL   Genes Dev 18 (21), 2573-2580 (2004)
   PUBMED   15520277
REFERENCE   6  (bases 1 to 5639)
  AUTHORS   Oberg,C., Li,J., Pauley,A., Wolf,E., Gurney,M. and Lendahl,U.
  TITLE     The Notch intracellular domain is ubiquitinated and negatively
            regulated by the mammalian Sel-10 homolog
  JOURNAL   J Biol Chem 276 (38), 35847-35853 (2001)
   PUBMED   11461910
REFERENCE   7  (bases 1 to 5639)
  AUTHORS   Moberg,K.H., Bell,D.W., Wahrer,D.C., Haber,D.A. and Hariharan,I.K.
  TITLE     Archipelago regulates Cyclin E levels in Drosophila and is mutated
            in human cancer cell lines
  JOURNAL   Nature 413 (6853), 311-316 (2001)
   PUBMED   11565033
REFERENCE   8  (bases 1 to 5639)
  AUTHORS   Gupta-Rossi,N., Le Bail,O., Gonen,H., Brou,C., Logeat,F., Six,E.,
            Ciechanover,A. and Israel,A.
  TITLE     Functional interaction between SEL-10, an F-box protein, and the
            nuclear form of activated Notch1 receptor
  JOURNAL   J Biol Chem 276 (37), 34371-34378 (2001)
   PUBMED   11425854
REFERENCE   9  (bases 1 to 5639)
  AUTHORS   Winston,J.T., Koepp,D.M., Zhu,C., Elledge,S.J. and Harper,J.W.
  TITLE     A family of mammalian F-box proteins
  JOURNAL   Curr Biol 9 (20), 1180-1182 (1999)
   PUBMED   10531037
REFERENCE   10 (bases 1 to 5639)
  AUTHORS   Robertson,N.G., Khetarpal,U., Gutierrez-Espeleta,G.A., Bieber,F.R.
            and Morton,C.C.
  TITLE     Isolation of novel and known genes from a human fetal cochlear cDNA
            library using subtractive hybridization and differential screening
  JOURNAL   Genomics 23 (1), 42-50 (1994)
   PUBMED   7829101
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC023424.7, BC143944.1,
            AC080078.7 and CA425646.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 16, 2018 this sequence version replaced NM_001349798.1.
            
            Summary: This gene encodes a member of the F-box protein family
            which is characterized by an approximately 40 amino acid motif, the
            F-box. The F-box proteins constitute one of the four subunits of
            ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box),
            which function in phosphorylation-dependent ubiquitination. The
            F-box proteins are divided into 3 classes: Fbws containing WD-40
            domains, Fbls containing leucine-rich repeats, and Fbxs containing
            either different protein-protein interaction modules or no
            recognizable motifs. The protein encoded by this gene was
            previously referred to as FBX30, and belongs to the Fbws class; in
            addition to an F-box, this protein contains 7 tandem WD40 repeats.
            This protein binds directly to cyclin E and probably targets cyclin
            E for ubiquitin-mediated degradation. Mutations in this gene are
            detected in ovarian and breast cancer cell lines, implicating the
            gene's potential role in the pathogenesis of human cancers.
            Multiple transcript variants encoding different isoforms have been
            found for this gene. [provided by RefSeq, Mar 2012].
            
            Transcript Variant: This variant (5) differs in the 5' UTR compared
            to variant 1. Variants 1 and 5 encode the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853567.23508.1,
                                           SRR14038194.3332607.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000281708.10/ ENSP00000281708.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-966               AC023424.7         112903-113868       c
            967-998             AC023424.7         112778-112809       c
            999-3560            BC143944.1         1-2562
            3561-4242           AC080078.7         48284-48965         c
            4243-4925           CA425646.1         18-700              c
            4926-5639           AC080078.7         46887-47600         c
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q31.3"
     gene            1..5639
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /note="F-box and WD repeat domain containing 7"
                     /db_xref="GeneID:55294"
                     /db_xref="HGNC:HGNC:16712"
                     /db_xref="MIM:606278"
     exon            1..966
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1059
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1109
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1679
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /inference="alignment:Splign:2.1.0"
     CDS             1179..3302
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /note="isoform 1 is encoded by transcript variant 5; F-box
                     protein SEL-10; archipelago; homolog of C elegans sel-10;
                     F-box protein FBW7; F-box and WD repeat domain containing
                     7, E3 ubiquitin protein ligase; F-box and WD-40 domain
                     protein 7 (archipelago homolog, Drosophila); F-box protein
                     FBX30"
                     /codon_start=1
                     /product="F-box/WD repeat-containing protein 7 isoform 1"
                     /protein_id="NP_001336727.1"
                     /db_xref="CCDS:CCDS3777.1"
                     /db_xref="GeneID:55294"
                     /db_xref="HGNC:HGNC:16712"
                     /db_xref="MIM:606278"
                     /translation="MNQELLSVGSKRRRTGGSLRGNPSSSQVDEEQMNRVVEEEQQQQ
                     LRQQEEEHTARNGEVVGVEPRPGGQNDSQQGQLEENNNRFISVDEDSSGNQEEQEEDE
                     EHAGEQDEEDEEEEEMDQESDDFDQSDDSSREDEHTHTNSVTNSSSIVDLPVHQLSSP
                     FYTKTTKMKRKLDHGSEVRSFSLGKKPCKVSEYTSTTGLVPCSATPTTFGDLRAANGQ
                     GQQRRRITSVQPPTGLQEWLKMFQSWSGPEKLLALDELIDSCEPTQVKHMMQVIEPQF
                     QRDFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCKEEGIDEPL
                     HIKRRKVIKPGFIHSPWKSAYIRQHRIDTNWRRGELKSPKVLKGHDDHVITCLQFCGN
                     RIVSGSDDNTLKVWSAVTGKCLRTLVGHTGGVWSSQMRDNIIISGSTDRTLKVWNAET
                     GECIHTLYGHTSTVRCMHLHEKRVVSGSRDATLRVWDIETGQCLHVLMGHVAAVRCVQ
                     YDGRRVVSGAYDFMVKVWDPETETCLHTLQGHTNRVYSLQFDGIHVVSGSLDTSIRVW
                     DVETGNCIHTLTGHQSLTSGMELKDNILVSGNADSTVKIWDIKTGQCLQTLQGPNKHQ
                     SAVTCLQFNKNFVITSSDDGTVKLWDLKTGEFIRNLVTLESGGSGGVVWRIRASNTKL
                     VCAVGSRNGTEETKLLVLDFDVDMK"
     exon            1680..1762
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /inference="alignment:Splign:2.1.0"
     exon            1763..1904
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..2039
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /inference="alignment:Splign:2.1.0"
     exon            2040..2163
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /inference="alignment:Splign:2.1.0"
     exon            2164..2300
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /inference="alignment:Splign:2.1.0"
     exon            2301..2414
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /inference="alignment:Splign:2.1.0"
     exon            2415..2596
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /inference="alignment:Splign:2.1.0"
     exon            2597..2822
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /inference="alignment:Splign:2.1.0"
     exon            2823..3033
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /inference="alignment:Splign:2.1.0"
     exon            3034..5639
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4902..4907
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /note="hexamer: AATAAA"
     polyA_site      4925
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /note="major polyA site"
     regulatory      5618..5623
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
                     /note="hexamer: AATAAA"
     polyA_site      5639
                     /gene="FBXW7"
                     /gene_synonym="AGO; CDC4; DEDHIL; FBW6; FBW7; FBX30;
                     FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10"
ORIGIN      
        1 ctctctggag tgaggcgaga gccccgcaca gagcgaggga gacagcgagc tgagctccgg
       61 gcgctgccgc tgccgctgcc gccgccgccg ccgctgagac tgagagcgaa ggagcatccg
      121 agagatccag tccccctgca ctggccgccg ccgagacctt cgctctcacc tgggccagcg
      181 ggagccgcgg ccgcactcct ttccccccct caccttcccg gccggcagcg gcggctgcac
      241 acgccggagc cggagccaga gccggagccc gagcctgagc cggagccggc ggcttggggg
      301 gcagggaggc ggctaccacg ggccgggagt gggtagctgc tccgcggtga gagaacgctg
      361 aggaggcgcc agagcttctg cctcgtcccg tggggcgtgg ggcgagaccc ccaaggtgta
      421 gggagggggg tcccagccgc agcgacacat gcgggagccg ggagcggggg cggcgccgag
      481 cggagccggc cgggtccctc gccttgccgc cgactcggcc acccgcccgg ggccgtagca
      541 tcttgccccg gagtgtatga accggggccc caaccaagct cggcaaccac cccccggccg
      601 ggggggcggg gaccccgatg tgaagcggcg gctggggcgg cggagagaac aggaccgacg
      661 ccgccgtcct ttcctcacct tccccctccc ctcagccccc tccgggggtc ttctcccttg
      721 gccagtcgcc ggccccccgg ctccttggct ggactccggg aggagttcct agagcccccc
      781 tcccccgccc cagtcccgag ggcggcgggg ccggggggga cccggggggc cggccgcagc
      841 ctccacccag aggaaacttt acaaaaacaa aatccggagt ctcccaaacc tgactgtccc
      901 gggagaagtg gccctggacg ggcagaagcc gcagcctgaa aagacccagg aagaggaaaa
      961 gaggagtacc gcgccggagc cttccgcagc tgccgcttca gtccgaagga ggaagggaac
     1021 caacccactt tctcggcgcc gcggctcttt tctaaaagtg attacttcct taggatagat
     1081 tgccagaagt ggagttactg ggtcagagga atgtgaaaac ctttgcatct tctgatagtc
     1141 tagccaaggt ccaagaagta gcaagctggc ttttggaaat gaatcaggaa ctgctctctg
     1201 tgggcagcaa aagacgacga actggaggct ctctgagagg taacccttcc tcaagccagg
     1261 tagatgaaga acagatgaat cgtgtggtag aggaggaaca gcaacagcaa ctcagacaac
     1321 aagaggagga gcacactgca aggaatggtg aagttgttgg agtagaacct agacctggag
     1381 gccaaaatga ttcccagcaa ggacagttgg aagaaaacaa taatagattt atttcggtag
     1441 atgaggactc ctcaggaaac caagaagaac aagaggaaga tgaagaacat gctggtgaac
     1501 aagatgagga ggatgaggag gaggaggaga tggaccagga gagtgacgat tttgatcagt
     1561 ctgatgatag tagcagagaa gatgaacata cacatactaa cagtgtcacg aactccagta
     1621 gtattgtgga cctgcccgtt caccaactct cctccccatt ctatacaaaa acaacaaaaa
     1681 tgaaaagaaa gttggaccat ggttctgagg tccgctcttt ttctttggga aagaaaccat
     1741 gcaaagtctc agaatataca agtaccactg ggcttgtacc atgttcagca acaccaacaa
     1801 cttttgggga cctcagagca gccaatggcc aagggcaaca acgacgccga attacatctg
     1861 tccagccacc tacaggcctc caggaatggc taaaaatgtt tcagagctgg agtggaccag
     1921 agaaattgct tgctttagat gaactcattg atagttgtga accaacacaa gtaaaacata
     1981 tgatgcaagt gatagaaccc cagtttcaac gagacttcat ttcattgctc cctaaagagt
     2041 tggcactcta tgtgctttca ttcctggaac ccaaagacct gctacaagca gctcagacat
     2101 gtcgctactg gagaattttg gctgaagaca accttctctg gagagagaaa tgcaaagaag
     2161 aggggattga tgaaccattg cacatcaaga gaagaaaagt aataaaacca ggtttcatac
     2221 acagtccatg gaaaagtgca tacatcagac agcacagaat tgatactaac tggaggcgag
     2281 gagaactcaa atctcctaag gtgctgaaag gacatgatga tcatgtgatc acatgcttac
     2341 agttttgtgg taaccgaata gttagtggtt ctgatgacaa cactttaaaa gtttggtcag
     2401 cagtcacagg caaatgtctg agaacattag tgggacatac aggtggagta tggtcatcac
     2461 aaatgagaga caacatcatc attagtggat ctacagatcg gacactcaaa gtgtggaatg
     2521 cagagactgg agaatgtata cacaccttat atgggcatac ttccactgtg cgttgtatgc
     2581 atcttcatga aaaaagagtt gttagcggtt ctcgagatgc cactcttagg gtttgggata
     2641 ttgagacagg ccagtgttta catgttttga tgggtcatgt tgcagcagtc cgctgtgttc
     2701 aatatgatgg caggagggtt gttagtggag catatgattt tatggtaaag gtgtgggatc
     2761 cagagactga aacctgtcta cacacgttgc aggggcatac taatagagtc tattcattac
     2821 agtttgatgg tatccatgtg gtgagtggat ctcttgatac atcaatccgt gtttgggatg
     2881 tggagacagg gaattgcatt cacacgttaa cagggcacca gtcgttaaca agtggaatgg
     2941 aactcaaaga caatattctt gtctctggga atgcagattc tacagttaaa atctgggata
     3001 tcaaaacagg acagtgttta caaacattgc aaggtcccaa caagcatcag agtgctgtga
     3061 cctgtttaca gttcaacaag aactttgtaa ttaccagctc agatgatgga actgtaaaac
     3121 tatgggactt gaaaacgggt gaatttattc gaaacctagt cacattggag agtgggggga
     3181 gtgggggagt tgtgtggcgg atcagagcct caaacacaaa gctggtgtgt gcagttggga
     3241 gtcggaatgg gactgaagaa accaagctgc tggtgctgga ctttgatgtg gacatgaagt
     3301 gaagagcaga aaagatgaat ttgtccaatt gtgtagacga tatactccct gcccttcccc
     3361 ctgcaaaaag aaaaaaagaa aagaaaaaga aaaaaatccc ttgttctcag tggtgcagga
     3421 tgttggcttg gggcaacaga ttgaaaagac ctacagacta agaaggaaaa gaagaagaga
     3481 tgacaaacca taactgacaa gagaggcgtc tgctgtctca tcacataaaa ggcttcactt
     3541 ttgactgagg gcagctttgc aaaatgagac tttctaaatc aaaccaggtg caattatttc
     3601 tttattttct tctccagtgg tcattgggca gtgttaatgc tgaaacatca ttacagattc
     3661 tgctagcctg ttcttttacc actgacagct agacacctag aaaggaactg caataatatc
     3721 aaaacaagta ctggttgact ttctaattag agagcatctg caacaaaaag tcatttttct
     3781 ggagtggaaa agcttaaaaa aattactgtg aattgttttt gtacagttat catgaaaagc
     3841 tttttttttt ttttttttgc caaccattgc caatgtcaat caatcacagt attagcctct
     3901 gttaatctat ttactgttgc ttccatatac attcttcaat gcatatgttg ctcaaaggtg
     3961 gcaagttgtc ctgggttctg tgagtcctga gatggattta attcttgatg ctggtgctag
     4021 aagtaggtct tcaaatatgg gattgttgtc ccaaccctgt actgtactcc cagtggccaa
     4081 acttatttat gctgctaaat gaaagaaaga aaaaagcaaa ttattttttt ttattttttt
     4141 tctgctgtga cgttttagtc ccagactgaa ttccaaattt gctctagttt ggttatggaa
     4201 aaaagacttt ttgccactga aacttgagcc atctgtgcct ctaagaggct gagaatggaa
     4261 gagtttcaga taataaagag tgaagtttgc ctgcaagtaa agaattgaga gtgtgtgcaa
     4321 agcttatttt cttttatctg ggcaaaaatt aaaacacatt ccttggaaca gagctattac
     4381 ttgcctgttc tgtggagaaa cttttctttt tgagggctgt ggtgaatgga tgaacgtaca
     4441 tcgtaaaact gacaaaatat tttaaaaata tataaaacac aaaattaaaa taaagttgct
     4501 ggtcagtctt agtgttttac agtatttggg aaaacaactg ttacagtttt attgctctga
     4561 gtaactgaca aagcagaaac tattcagttt ttgtagtaaa ggcgtcacat gcaaacaaac
     4621 aaaatgaatg aaacagtcaa atggtttgcc tcattctcca agagccacaa ctcaagctga
     4681 actgtgaaag tggtttaaca ctgtatccta ggcgatcttt tttcctcctt ctgtttattt
     4741 ttttgtttgt tttatttata gtctgattta aaacaatcag attcaagttg gttaatttta
     4801 gttatgtaac aacctgacat gatggaggaa aacaaccttt aaagggattg tgtctatggt
     4861 ttgattcact tagaaatttt attttcttat aacttaagtg caataaaatg tgttttttca
     4921 tgttagtatg cctgtttctt ccactgaagt aataatcctt cattataagt atttaggatt
     4981 tgtctataat atttaaataa gcaaaatctt ggtttctttg catcctgctg tgtgacatag
     5041 aaatcaatac ttcaagcctg ttttataccg cgaaagtaag gataatgtgc tgcaactgca
     5101 aattttattt ctctcctaga ctatgaagga ggacactcca ctgtgctcat ctctgtagat
     5161 tgcattgggg tgtataaata tgaaatctgg ttccctcaaa gaatccacaa aacttttgtg
     5221 ataattacaa ctagaagcct tataaataac aaattcattt tgaaattttg accacatatt
     5281 aagtgcttac cctcgatact ttctgttcat tctctcttaa gctcccacta tccctgagaa
     5341 gtaggcacta ttatctccca tcttaaagac gagcaaaaat ctgcagctga gactggttag
     5401 gcaatttgtc aaatcataga actagcggaa tgccagaact caaacccata tttccaaaga
     5461 ctaaatacat gctgctcttt aagaaagttt gacagactaa agcaaggttt gagagggaaa
     5521 taagccattt ttctggtact ttctgactgt tctgtagccg ttttgttcaa caaatctaaa
     5581 ttttataaaa tctaaatttt atgatgtaag gggaaaaaat aaagttgttt ttgaaacta
//
