LOCUS       NM_001142522            2215 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens F-box protein 5 (FBXO5), transcript variant 2, mRNA.
ACCESSION   NM_001142522
VERSION     NM_001142522.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Campos Gudino,R., Neudorf,N.M., Andromidas,D., Lichtensztejn,Z. and
            McManus,K.J.
  TITLE     Loss of EMI1 compromises chromosome stability and is associated
            with cellular transformation in colonic epithelial cell contexts
  JOURNAL   Br J Cancer 131 (9), 1516-1528 (2024)
   PUBMED   39358461
  REMARK    GeneRIF: Loss of EMI1 compromises chromosome stability and is
            associated with cellular transformation in colonic epithelial cell
            contexts.
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Kuang,Y., Huang,S., Tang,S., Zhuo,Z. and Linghu,K.
  TITLE     Effect of Emi1 gene silencing on the proliferation and invasion of
            human breast cancer cells
  JOURNAL   BMC Mol Cell Biol 24 (1), 34 (2023)
   PUBMED   38041032
  REMARK    GeneRIF: Effect of Emi1 gene silencing on the proliferation and
            invasion of human breast cancer cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2215)
  AUTHORS   Zhang,J., Zhang,G., Wang,K., Cui,F., Yang,H. and Jiao,Z.
  TITLE     Exploring the role of FBXO5 in gastric cancer
  JOURNAL   Mol Cell Probes 69, 101915 (2023)
   PUBMED   37121410
  REMARK    GeneRIF: Exploring the role of FBXO5 in gastric cancer.
REFERENCE   4  (bases 1 to 2215)
  AUTHORS   Li,H., Yang,C., Chen,K. and Sun,M.
  TITLE     Expression significance of Emi1, UBCH10 and CyclinB1 in esophageal
            squamous cell carcinoma
  JOURNAL   Pathol Oncol Res 29, 1611081 (2023)
   PUBMED   37168048
  REMARK    GeneRIF: Expression significance of Emi1, UBCH10 and CyclinB1 in
            esophageal squamous cell carcinoma.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2215)
  AUTHORS   Reimann,J.D. and Jackson,P.K.
  TITLE     Emi1 is required for cytostatic factor arrest in vertebrate eggs
  JOURNAL   Nature 416 (6883), 850-854 (2002)
   PUBMED   11976684
REFERENCE   6  (bases 1 to 2215)
  AUTHORS   Reimann,J.D., Freed,E., Hsu,J.Y., Kramer,E.R., Peters,J.M. and
            Jackson,P.K.
  TITLE     Emi1 is a mitotic regulator that interacts with Cdc20 and inhibits
            the anaphase promoting complex
  JOURNAL   Cell 105 (5), 645-655 (2001)
   PUBMED   11389834
REFERENCE   7  (bases 1 to 2215)
  AUTHORS   Chiaur,D.S., Murthy,S., Cenciarelli,C., Parks,W., Loda,M.,
            Inghirami,G., Demetrick,D. and Pagano,M.
  TITLE     Five human genes encoding F-box proteins: chromosome mapping and
            analysis in human tumors
  JOURNAL   Cytogenet Cell Genet 88 (3-4), 255-258 (2000)
   PUBMED   10828603
REFERENCE   8  (bases 1 to 2215)
  AUTHORS   Winston,J.T., Koepp,D.M., Zhu,C., Elledge,S.J. and Harper,J.W.
  TITLE     A family of mammalian F-box proteins
  JOURNAL   Curr Biol 9 (20), 1180-1182 (1999)
   PUBMED   10531037
REFERENCE   9  (bases 1 to 2215)
  AUTHORS   Cenciarelli,C., Chiaur,D.S., Guardavaccaro,D., Parks,W., Vidal,M.
            and Pagano,M.
  TITLE     Identification of a family of human F-box proteins
  JOURNAL   Curr Biol 9 (20), 1177-1179 (1999)
   PUBMED   10531035
REFERENCE   10 (bases 1 to 2215)
  AUTHORS   Gubin,A.N., Njoroge,J.M., Bouffard,G.G. and Miller,J.L.
  TITLE     Gene expression in proliferating human erythroid cells
  JOURNAL   Genomics 59 (2), 168-177 (1999)
   PUBMED   10409428
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI838832.1, BC018905.1,
            AJ420499.1 and AF129535.2.
            
            On May 31, 2019 this sequence version replaced NM_001142522.2.
            
            Summary: This gene encodes a member of the F-box protein family
            which is characterized by an approximately 40 amino acid motif, the
            F-box. The F-box proteins constitute one of the four subunits of
            the ubiquitin protein ligase complex called SCFs
            (SKP1-cullin-F-box), which function in phosphorylation-dependent
            ubiquitination. The F-box proteins are divided into 3 classes: Fbws
            containing WD-40 domains, Fbls containing leucine-rich repeats, and
            Fbxs containing either different protein-protein interaction
            modules or no recognizable motifs. The protein encoded by this gene
            belongs to the Fbxs class. This protein is similar to xenopus early
            mitotic inhibitor-1 (Emi1), which is a mitotic regulator that
            interacts with Cdc20 and inhibits the anaphase promoting complex.
            Alternatively spliced transcript variants encoding different
            isoforms have been identified. [provided by RefSeq, Dec 2008].
            
            Transcript Variant: This variant (2) contains an alternate exon in
            the 5' coding region and uses a downstream start codon, compared to
            variant 1. Isoform b has a shorter N-terminus, compared to isoform
            a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK055221.1, AJ420499.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03267767, SAMN03267770
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-346               BI838832.1         58-403
            347-561             BC018905.1         155-369
            562-2210            AJ420499.1         448-2096
            2211-2215           AF129535.2         2030-2034
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q25.2"
     gene            1..2215
                     /gene="FBXO5"
                     /gene_synonym="EMI1; FBX5; Fbxo31"
                     /note="F-box protein 5"
                     /db_xref="GeneID:26271"
                     /db_xref="HGNC:HGNC:13584"
                     /db_xref="MIM:606013"
     exon            1..346
                     /gene="FBXO5"
                     /gene_synonym="EMI1; FBX5; Fbxo31"
                     /inference="alignment:Splign:2.1.0"
     exon            347..1061
                     /gene="FBXO5"
                     /gene_synonym="EMI1; FBX5; Fbxo31"
                     /inference="alignment:Splign:2.1.0"
     CDS             382..1587
                     /gene="FBXO5"
                     /gene_synonym="EMI1; FBX5; Fbxo31"
                     /note="isoform b is encoded by transcript variant 2; F-box
                     protein Fbx5; F-box only protein 5; early mitotic
                     inhibitor 1; early fission inhibitory protein 1"
                     /codon_start=1
                     /product="F-box only protein 5 isoform b"
                     /protein_id="NP_001135994.1"
                     /db_xref="CCDS:CCDS47501.1"
                     /db_xref="GeneID:26271"
                     /db_xref="HGNC:HGNC:13584"
                     /db_xref="MIM:606013"
                     /translation="MKCDFNCNHVHSGLKLVKPDDIGRLVSYTPAYLEGSCKDCIKDY
                     ERLSCIGSPIVSPRIVQLETESKRLHNKENQHVQQTLNSTNEIEALETSRLYEDSGYS
                     SFSLQSGLSEHEEGSLLEENFGDSLQSCLLQIQSPDQYPNKNLLPVLHFEKVVCSTLK
                     KNAKRNPKVDREMLKEIIARGNFRLQNIIGRKMGLECVDILSELFRRGLRHVLATILA
                     QLSDMDLINVSKVSTTWKKILEDDKGAFQLYSKAIQRVTENNNKFSPHASTREYVMFR
                     TPLASVQKSAAQTSLKKDAQTKLSNQGDQKGSTYSRHNEFSEVAKTLKKNESLKACIR
                     CNSPAKYDCYLQRATCKREGCGFDYCTKCLCNYHTTKDCSDGKLLKASCKIGPLPGTK
                     KSKKNLRRL"
     exon            1062..1152
                     /gene="FBXO5"
                     /gene_synonym="EMI1; FBX5; Fbxo31"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1335
                     /gene="FBXO5"
                     /gene_synonym="EMI1; FBX5; Fbxo31"
                     /inference="alignment:Splign:2.1.0"
     exon            1336..2215
                     /gene="FBXO5"
                     /gene_synonym="EMI1; FBX5; Fbxo31"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2195..2200
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FBXO5"
                     /gene_synonym="EMI1; FBX5; Fbxo31"
                     /note="hexamer: AATAAA"
     polyA_site      2215
                     /gene="FBXO5"
                     /gene_synonym="EMI1; FBX5; Fbxo31"
                     /note="major polyA site"
ORIGIN      
        1 gtaaatccta gagaggcggg ctaagctgga ctggggggag ggtccgtctt ccggaaagtc
       61 tggattcccg gacgagccga gttgctgctc accgaactcc cgttcgagag atgatcgaag
      121 aaagtcggct accatttgta cccatcaaag atctccagat ggaagccagc gctgaatttg
      181 ggctgagatt aggacttgca ggaggccggt ccagaagacg gcggaaggaa tcttggcggg
      241 cgcacgcatg cgtgatagac cctccacacg tgtggccggg ccgcggcctc cccgtgctcg
      301 gaggtcccgc ccccggccgt agcatctttc cggacgtggg gagccggttg taaagaagaa
      361 agttctaccc tttctgtcaa aatgaagtgt gattttaatt gtaaccatgt tcattccgga
      421 cttaaactgg taaaacctga tgacattgga agactagttt cctacacccc tgcatatttg
      481 gaaggttcct gtaaagactg cattaaagac tatgaaaggc tgtcatgtat tgggtcaccg
      541 attgtgagcc ctaggattgt acaacttgaa actgaaagca agcgcttgca taacaaggaa
      601 aatcaacatg tgcaacagac acttaatagt acaaatgaaa tagaagcact agagaccagt
      661 agactttatg aagacagtgg ctattcctca ttttctctac aaagtggcct cagtgaacat
      721 gaagaaggta gcctcctgga ggagaatttc ggtgacagtc tacaatcctg cctgctacaa
      781 atacaaagcc cagaccaata tcccaacaaa aacttgctgc cagttcttca ttttgaaaaa
      841 gtggtttgtt caacattaaa aaagaatgca aaacgaaatc ctaaagtaga tcgggagatg
      901 ctgaaggaaa ttatagccag aggaaatttt agactgcaga atataattgg cagaaaaatg
      961 ggcctagaat gtgtagatat tctcagcgaa ctctttcgaa ggggactcag acatgtctta
     1021 gcaactattt tagcacaact cagtgacatg gacttaatca atgtgtctaa agtgagcaca
     1081 acttggaaga agatcctaga agatgataag ggggcattcc agttgtacag taaagcaata
     1141 caaagagtta ccgaaaacaa caataaattt tcacctcatg cttcaaccag agaatatgtt
     1201 atgttcagaa ccccactggc ttctgttcag aaatcagcag cccagacttc tctcaaaaaa
     1261 gatgctcaaa ccaagttatc caatcaaggt gatcagaaag gttctactta tagtcgacac
     1321 aatgaattct ctgaggttgc caagacattg aaaaagaacg aaagcctcaa agcctgtatt
     1381 cgctgtaatt cacctgcaaa atatgattgc tatttacaac gggcaacctg caaacgagaa
     1441 ggctgtggat ttgattattg tacgaagtgt ctctgtaatt atcatactac taaagactgt
     1501 tcagatggca agctcctcaa agccagttgt aaaataggtc ccctgcctgg tacaaagaaa
     1561 agcaaaaaga atttacgaag attgtgatct cttattaaat caattgttac tgatcatgaa
     1621 tgttagttag aaaatgttag gttttaactt aaaaaaaatt gtattgtgat tttcaatttt
     1681 atgttgaaat cggtgtagta tcctgaggtt tttttccccc cagaagataa agaggataga
     1741 caacctctta aaatattttt acaatttaat gagaaaaagt ttaaaattct caatacaaat
     1801 caaacaattt aaatatttta agaaaaaagg aaaagtagat agtgatactg agggtaaaaa
     1861 aaaattgatt caattttatg gtaaaggaaa cccatgcaat tttacctaga cagtcttaaa
     1921 tatgtctggt tttccatctg ttagcatttc agacatttta tgttcctctt actcaattga
     1981 taccaacaga aatatcaact tctggagtct attaaatgtg ttgtcacctt tctaaagctt
     2041 tttttcattg tgtgtatttc ccaagaaagt atcctttgta aaaacttgct tgttttcctt
     2101 atttctgaaa tctgttttaa tatttttgta tacatgtaaa tatttctgta ttttttatat
     2161 gtcaaagaat atgtctcttg tatgtacata taaaaataaa ttttgctcaa taaaa
//
