LOCUS       NP_036308                387 aa            linear   PRI 20-SEP-2024
DEFINITION  F-box only protein 4 isoform 1 [Homo sapiens].
ACCESSION   NP_036308
VERSION     NP_036308.1
DBSOURCE    REFSEQ: accession NM_012176.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 387)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (residues 1 to 387)
  AUTHORS   Liu,J., Yang,H., Cheung,P.Y., Tsao,S.W., Lv,L. and Cheung,A.L.M.
  TITLE     FBX4 mediates rapid cyclin D1 proteolysis upon DNA damage in
            immortalized esophageal epithelial cells
  JOURNAL   Biochem Biophys Res Commun 554, 76-82 (2021)
   PUBMED   33784509
  REMARK    GeneRIF: FBX4 mediates rapid cyclin D1 proteolysis upon DNA damage
            in immortalized esophageal epithelial cells.
REFERENCE   3  (residues 1 to 387)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (residues 1 to 387)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (residues 1 to 387)
  AUTHORS   Qie,S., Majumder,M., Mackiewicz,K., Howley,B.V., Peterson,Y.K.,
            Howe,P.H., Palanisamy,V. and Diehl,J.A.
  TITLE     Fbxo4-mediated degradation of Fxr1 suppresses tumorigenesis in head
            and neck squamous cell carcinoma
  JOURNAL   Nat Commun 8 (1), 1534 (2017)
   PUBMED   29142209
  REMARK    GeneRIF: in head and neck squamous cell carcinoma, fragile X mental
            retardation syndrome related protein 1 (Fxr1) overexpression
            correlates with reduced F-box protein 4 (Fbxo4) levels.
            Publication Status: Online-Only
REFERENCE   6  (residues 1 to 387)
  AUTHORS   Jin,J., Cardozo,T., Lovering,R.C., Elledge,S.J., Pagano,M. and
            Harper,J.W.
  TITLE     Systematic analysis and nomenclature of mammalian F-box proteins
  JOURNAL   Genes Dev 18 (21), 2573-2580 (2004)
   PUBMED   15520277
REFERENCE   7  (residues 1 to 387)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   8  (residues 1 to 387)
  AUTHORS   Chiaur,D.S., Murthy,S., Cenciarelli,C., Parks,W., Loda,M.,
            Inghirami,G., Demetrick,D. and Pagano,M.
  TITLE     Five human genes encoding F-box proteins: chromosome mapping and
            analysis in human tumors
  JOURNAL   Cytogenet Cell Genet 88 (3-4), 255-258 (2000)
   PUBMED   10828603
REFERENCE   9  (residues 1 to 387)
  AUTHORS   Winston,J.T., Koepp,D.M., Zhu,C., Elledge,S.J. and Harper,J.W.
  TITLE     A family of mammalian F-box proteins
  JOURNAL   Curr Biol 9 (20), 1180-1182 (1999)
   PUBMED   10531037
REFERENCE   10 (residues 1 to 387)
  AUTHORS   Cenciarelli,C., Chiaur,D.S., Guardavaccaro,D., Parks,W., Vidal,M.
            and Pagano,M.
  TITLE     Identification of a family of human F-box proteins
  JOURNAL   Curr Biol 9 (20), 1177-1179 (1999)
   PUBMED   10531035
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK024075.1, BC040011.1,
            BC048098.1 and AC034222.5.
            
            Summary: This gene encodes a member of the F-box protein family
            which is characterized by an approximately 40 amino acid motif, the
            F-box. The F-box proteins constitute one of the four subunits of
            the ubiquitin protein ligase complex called SCFs
            (SKP1-cullin-F-box), which function in phosphorylation-dependent
            ubiquitination. The F-box proteins are divided into 3 classes: Fbws
            containing WD-40 domains, Fbls containing leucine-rich repeats, and
            Fbxs containing either different protein-protein interaction
            modules or no recognizable motifs. The protein encoded by this gene
            belongs to the Fbxs class. Alternatively spliced transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.123476.1, AK024075.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000281623.8/ ENSP00000281623.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p13.1"
     Protein         1..387
                     /product="F-box only protein 4 isoform 1"
                     /note="F-box protein Fbx4; F-box only protein 4"
                     /calculated_mol_wt=44005
     Site            12
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000269|PubMed:18598945,
                     ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:23186163,
                     ECO:0007744|PubMed:24275569; propagated from
                     UniProtKB/Swiss-Prot (Q9UKT5.2)"
     Site            48
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q9UKT5.2)"
     Region          57..106
                     /region_name="F-box_FBXO4"
                     /note="F-box domain found in F-box only protein 4 (FBXO4)
                     and similar proteins; cd22085"
                     /db_xref="CDD:438857"
     Site            order(57..60,62,65..66,69..70,73..74,76,78,80..87,89..90,
                     94)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438857"
     Region          162..384
                     /region_name="FBX4_GTPase_like"
                     /note="C-terminal GTPase-like domain of F-Box Only Protein
                     4; cd11656"
                     /db_xref="CDD:212555"
     Site            order(165,171)
                     /site_type="other"
                     /note="SKP1 binding interface [polypeptide binding]"
                     /db_xref="CDD:212555"
     Site            order(340,342..343,345,356..357,359,361)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:212555"
     Site            order(341..342,345..346,357..361,372)
                     /site_type="other"
                     /note="TRF1 binding interface [polypeptide binding]"
                     /db_xref="CDD:212555"
     CDS             1..387
                     /gene="FBXO4"
                     /gene_synonym="FBX4"
                     /coded_by="NM_012176.3:30..1193"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS3938.1"
                     /db_xref="GeneID:26272"
                     /db_xref="HGNC:HGNC:13583"
                     /db_xref="MIM:609090"
ORIGIN      
        1 magseprsgt nsppppfsdw grleaailsg wktfwqsvsk ervarttsre evdeaastlt
       61 rlpidvqlyi lsflsphdlc qlgstnhywn etvrdpilwr yfllrdlpsw ssvdwkslpd
      121 leilkkpise vtdgaffdym avyrmccpyt rraskssrpm ygavtsflhs liiqneprfa
      181 mfgpgleeln tslvlslmss eelcptaglp qrqidgigsg vnfqlnnqhk fnililystt
      241 rkerdraree htsavnkmfs rhnegddqqg srysvipqiq kvcevvdgfi yvanaeahkr
      301 hewqdefshi mamtdpafgs sgrpllvlsc isqgdvkrmp cfylahelhl nllnhpwlvq
      361 dteaetltgf lngiewilee veskrar
//
