LOCUS       NM_012175               2403 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens F-box protein 3 (FBXO3), transcript variant 1, mRNA.
ACCESSION   NM_012175
VERSION     NM_012175.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Xu,J., Guo,R., Wen,N., Li,L., Yi,Y., Chen,J., He,Z., Yang,J.,
            Xiao,Z.J. and Niu,M.
  TITLE     FBXO3 stabilizes USP4 and Twist1 to promote PI3K-mediated breast
            cancer metastasis
  JOURNAL   PLoS Biol 21 (12), e3002446 (2023)
   PUBMED   38134227
  REMARK    GeneRIF: FBXO3 stabilizes USP4 and Twist1 to promote PI3K-mediated
            breast cancer metastasis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2403)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2403)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2403)
  AUTHORS   Chandra,D., Londino,J., Alexander,S., Bednash,J.S., Zhang,Y.,
            Friedlander,R.M., Daskivich,G., Carlisle,D.L., Lariviere,W.R.,
            Nakassa,A.C.I., Ross,M., St Croix,C., Nyunoya,T., Sciurba,F.,
            Chen,B. and Mallampalli,R.K.
  TITLE     The SCFFBXO3 ubiquitin E3 ligase regulates inflammation in
            atherosclerosis
  JOURNAL   J Mol Cell Cardiol 126, 50-59 (2019)
   PUBMED   30448480
  REMARK    GeneRIF: FBXO3 potentiates vascular inflammation and
            atherosclerosis that can be effectively mitigated by a small
            molecule inhibitor.
REFERENCE   6  (bases 1 to 2403)
  AUTHORS   Ebstein,F., Lange,N., Urban,S., Seifert,U., Kruger,E. and
            Kloetzel,P.M.
  TITLE     Maturation of human dendritic cells is accompanied by functional
            remodelling of the ubiquitin-proteasome system
  JOURNAL   Int J Biochem Cell Biol 41 (5), 1205-1215 (2009)
   PUBMED   19028597
REFERENCE   7  (bases 1 to 2403)
  AUTHORS   Shima,Y., Shima,T., Chiba,T., Irimura,T., Pandolfi,P.P. and
            Kitabayashi,I.
  TITLE     PML activates transcription by protecting HIPK2 and p300 from
            SCFFbx3-mediated degradation
  JOURNAL   Mol Cell Biol 28 (23), 7126-7138 (2008)
   PUBMED   18809579
  REMARK    GeneRIF: Fbx3 formed SCF(Fbx3) ubiquitin ligase and promoted the
            degradation of HIPK2 and p300 by the ubiquitin-proteasome pathway
REFERENCE   8  (bases 1 to 2403)
  AUTHORS   Ilyin,G.P., Rialland,M., Pigeon,C. and Guguen-Guillouzo,C.
  TITLE     cDNA cloning and expression analysis of new members of the
            mammalian F-box protein family
  JOURNAL   Genomics 67 (1), 40-47 (2000)
   PUBMED   10945468
REFERENCE   9  (bases 1 to 2403)
  AUTHORS   Winston,J.T., Koepp,D.M., Zhu,C., Elledge,S.J. and Harper,J.W.
  TITLE     A family of mammalian F-box proteins
  JOURNAL   Curr Biol 9 (20), 1180-1182 (1999)
   PUBMED   10531037
REFERENCE   10 (bases 1 to 2403)
  AUTHORS   Cenciarelli,C., Chiaur,D.S., Guardavaccaro,D., Parks,W., Vidal,M.
            and Pagano,M.
  TITLE     Identification of a family of human F-box proteins
  JOURNAL   Curr Biol 9 (20), 1177-1179 (1999)
   PUBMED   10531035
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC039291.1 and BC046110.1.
            
            On Nov 23, 2018 this sequence version replaced NM_012175.3.
            
            Summary: This gene encodes a member of the F-box protein family
            which is characterized by an approximately 40 amino acid motif, the
            F-box. The F-box proteins constitute one of the four subunits of
            the ubiquitin protein ligase complex called SCFs
            (SKP1-cullin-F-box), which function in phosphorylation-dependent
            ubiquitination. The F-box proteins are divided into 3 classes: Fbws
            containing WD-40 domains, Fbls containing leucine-rich repeats, and
            Fbxs containing either different protein-protein interaction
            modules or no recognizable motifs. The protein encoded by this gene
            belongs to the Fbxs class. Alternative splicing of this gene
            generates 2 transcript variants diverging at the 3' end. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform (1)
            which includes a highly acidic D/E-rich domain and two poly-R
            pieces at the C-terminus.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.913039.1,
                                           SRR1660809.258614.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467147 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265651.8/ ENSP00000265651.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-747               BC039291.1         6-752
            748-1699            BC039291.1         2098-3049
            1700-2403           BC046110.1         1691-2394
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p13"
     gene            1..2403
                     /gene="FBXO3"
                     /gene_synonym="FBA; FBX3"
                     /note="F-box protein 3"
                     /db_xref="GeneID:26273"
                     /db_xref="HGNC:HGNC:13582"
                     /db_xref="MIM:609089"
     exon            1..127
                     /gene="FBXO3"
                     /gene_synonym="FBA; FBX3"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..1439
                     /gene="FBXO3"
                     /gene_synonym="FBA; FBX3"
                     /note="isoform 1 is encoded by transcript variant 1; F-box
                     protein FBX3; F-box only protein 3"
                     /codon_start=1
                     /product="F-box only protein 3 isoform 1"
                     /protein_id="NP_036307.2"
                     /db_xref="CCDS:CCDS7887.1"
                     /db_xref="GeneID:26273"
                     /db_xref="HGNC:HGNC:13582"
                     /db_xref="MIM:609089"
                     /translation="MAAMETETAPLTLESLPTDPLLLILSFLDYRDLINCCYVSRRLS
                     QLSSHDPLWRRHCKKYWLISEEEKTQKNQCWKSLFIDTYSDVGRYIDHYAAIKKAWDD
                     LKKYLEPRCPRMVLSLKEGAREEDLDAVEAQIGCKLPDDYRCSYRIHNGQKLVVPGLL
                     GSMALSNHYRSEDLLDVDTAAGGFQQRQGLKYCLPLTFCIHTGLSQYIAVEAAEGRNK
                     NEVFYQCPDQMARNPAAIDMFIIGATFTDWFTSYVKNVVSGGFPIIRDQIFRYVHDPE
                     CVATTGDITVSVSTSFLPELSSVHPPHYFFTYRIRIEMSKDALPEKACQLDSRYWRIT
                     NAKGDVEEVQGPGVVGEFPIISPGRVYEYTSCTTFSTTSGYMEGYYTFHFLYFKDKIF
                     NVAIPRFHMACPTFRVSIARLEMGPDEYEEMEEEEEEEEEEDEDDDSADMDESDEDDE
                     EERRRRVFDVPIRRRRCSRLF"
     exon            128..217
                     /gene="FBXO3"
                     /gene_synonym="FBA; FBX3"
                     /inference="alignment:Splign:2.1.0"
     exon            218..381
                     /gene="FBXO3"
                     /gene_synonym="FBA; FBX3"
                     /inference="alignment:Splign:2.1.0"
     exon            382..496
                     /gene="FBXO3"
                     /gene_synonym="FBA; FBX3"
                     /inference="alignment:Splign:2.1.0"
     exon            497..701
                     /gene="FBXO3"
                     /gene_synonym="FBA; FBX3"
                     /inference="alignment:Splign:2.1.0"
     exon            702..747
                     /gene="FBXO3"
                     /gene_synonym="FBA; FBX3"
                     /inference="alignment:Splign:2.1.0"
     exon            748..832
                     /gene="FBXO3"
                     /gene_synonym="FBA; FBX3"
                     /inference="alignment:Splign:2.1.0"
     exon            833..955
                     /gene="FBXO3"
                     /gene_synonym="FBA; FBX3"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1071
                     /gene="FBXO3"
                     /gene_synonym="FBA; FBX3"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1262
                     /gene="FBXO3"
                     /gene_synonym="FBA; FBX3"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..2403
                     /gene="FBXO3"
                     /gene_synonym="FBA; FBX3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2348..2353
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FBXO3"
                     /gene_synonym="FBA; FBX3"
                     /note="hexamer: AATGAA"
     polyA_site      2391
                     /gene="FBXO3"
                     /gene_synonym="FBA; FBX3"
                     /note="major polyA site"
     polyA_site      2403
                     /gene="FBXO3"
                     /gene_synonym="FBA; FBX3"
                     /note="major polyA site"
ORIGIN      
        1 agacctgcac cgggccaggc aagatggcgg ccatggagac cgagacggcg ccgctgaccc
       61 tagagtcgct gcccaccgat cccctgctcc tcatcttatc ctttttggac tatcgggatc
      121 taatcaactg ttgttatgtc agtcgaagac ttagccagct atcaagtcat gatccgctgt
      181 ggagaagaca ttgcaaaaaa tactggctga tatctgagga agagaaaaca cagaagaatc
      241 agtgttggaa atctctcttc atagatactt actctgatgt aggaagatac attgaccatt
      301 atgctgctat taaaaaggcc tgggatgatc tcaagaaata tttggagccc aggtgtcctc
      361 ggatggtttt atctctgaaa gagggtgctc gagaggaaga cctcgatgct gtggaagcgc
      421 agattggctg caagcttcct gacgattatc gatgttcata ccgaattcac aatggacaga
      481 agttagtggt tcctgggtta ttgggaagca tggcactgtc taatcactat cgttctgaag
      541 atttgttaga cgtcgataca gctgccggag gattccagca gagacaggga ctgaaatact
      601 gtctcccttt aactttttgc atacatactg gtttgagtca gtacatagca gtggaagctg
      661 cagagggccg aaacaaaaat gaagttttct accaatgtcc agaccaaatg gctcgaaatc
      721 cagctgctat tgacatgttt attataggtg ctacttttac tgactggttt acctcttatg
      781 tcaaaaatgt tgtatcaggt ggcttcccca tcatcagaga ccaaattttc agatatgttc
      841 acgatccaga atgtgtagca acaactgggg atattactgt gtcagtttcc acatcgtttc
      901 tgccagaact tagctctgta catccacccc actatttctt cacataccga atcaggattg
      961 aaatgtcaaa agatgcactt cctgagaagg cctgtcagtt ggacagtcgc tattggagaa
     1021 taacaaatgc taagggtgac gtggaagaag ttcaaggacc tggagtagtt ggtgaatttc
     1081 caatcatcag cccaggtcgg gtatatgaat acacaagctg taccacattc tctacaacat
     1141 caggatacat ggaaggatat tataccttcc attttcttta ctttaaagac aagatcttta
     1201 atgttgccat tccccgattc catatggcat gtccaacatt cagggtgtct atagcccgat
     1261 tggaaatggg tcctgatgaa tatgaagaga tggaagaaga ggaggaggag gaagaggagg
     1321 aagacgagga tgatgattca gcagatatgg atgaatcaga tgaagatgat gaagaggaga
     1381 gacggaggag agtctttgat gttcccattc gcagacgccg ctgctcacgc cttttttagc
     1441 aagccttctg ctgatggaag cactaggatg attctaggct gttaaataga tttctcaata
     1501 atgtaaataa ctaaattgtt ctctgcatat agcaggaaaa ctagcatgaa atattgtttc
     1561 aggccctggg ttctatgtga cactacatta ggaattggat tgtttgggtt tgctttgtgt
     1621 ttttgaggta gaggaagaaa tgggaatctt ttttttctct tccaggagtc agtggaagaa
     1681 tagttctcta gctaaggaat ggacatacct ttgttttaaa atattttata cttacaaaaa
     1741 tctagaaatg gagagggaac tgttttgaat aaggatttaa aatacctgca caaggataga
     1801 gagaaactat gtgactcatt ctgtgaaaag acttcttgca gttgtgagtt atttagaaat
     1861 gatcaaaatt tgtaattagg ctaatccatt tagtgattcc taatattttg tactcacaga
     1921 gaactaattg actaaacaac ttgaacgcta gtggtttgtc cttagacaat ctgtctttga
     1981 atttaaagtc tttatcgcta agaccttgac tttaaatttt tcatcactac aaccttgaat
     2041 ttaatttcag gtcttcaaca tgatgacctt ggatttaatt taaagtcttc aacactatgc
     2101 gctttatcat attattcaca gatgcatttt tgaaatgtag tatgtaaaag tatgtaacgt
     2161 gctgtttatt aacaaaagat tgttcacaac atctcatgta gtttaaattt gtaaatactg
     2221 cttctgtttt gtttctcctt tatacacttg actgtctttg tgataagtga catgaatttt
     2281 atgttaggat taagtatgtt ttcctgaaac ttggattttt tttgtaatta tataattgag
     2341 agttaagaat gaaatccttc aagtgttaaa aactcacatt ttaaaagcaa attttggttc
     2401 caa
//
