LOCUS       NM_015176               5427 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens F-box protein 28 (FBXO28), transcript variant 1, mRNA.
ACCESSION   NM_015176
VERSION     NM_015176.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Lee,C.Y., Hubrich,D., Varga,J.K., Schafer,C., Welzel,M.,
            Schumbera,E., Djokic,M., Strom,J.M., Schonfeld,J., Geist,J.L.,
            Polat,F., Gibson,T.J., Keller Valsecchi,C.I., Kumar,M.,
            Schueler-Furman,O. and Luck,K.
  TITLE     Systematic discovery of protein interaction interfaces using
            AlphaFold and experimental validation
  JOURNAL   Mol Syst Biol 20 (2), 75-97 (2024)
   PUBMED   38225382
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Song,G., Sun,Z., Chu,M., Zhang,Z., Chen,J., Wang,Z. and Zhu,X.
  TITLE     FBXO28 promotes cell proliferation, migration and invasion via
            upregulation of the TGF-beta1/SMAD2/3 signaling pathway in ovarian
            cancer
  JOURNAL   BMC Cancer 24 (1), 122 (2024)
   PUBMED   38267923
  REMARK    GeneRIF: FBXO28 promotes cell proliferation, migration and invasion
            via upregulation of the TGF-beta1/SMAD2/3 signaling pathway in
            ovarian cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5427)
  AUTHORS   Qiao,X., Lin,J., Shen,J., Chen,Y., Zheng,L., Ren,H., Zhao,X.,
            Yang,H., Li,P. and Wang,Z.
  TITLE     FBXO28 suppresses liver cancer invasion and metastasis by promoting
            PKA-dependent SNAI2 degradation
  JOURNAL   Oncogene 42 (39), 2878-2891 (2023)
   PUBMED   37596321
  REMARK    GeneRIF: FBXO28 suppresses liver cancer invasion and metastasis by
            promoting PKA-dependent SNAI2 degradation.
REFERENCE   4  (bases 1 to 5427)
  AUTHORS   Liu,S., Liu,P., Zhu,C., Yang,R., He,Z., Li,Y., Li,Y., Fei,X.,
            Hou,J., Wang,X. and Pan,Y.
  TITLE     FBXO28 promotes proliferation, invasion, and metastasis of
            pancreatic cancer cells through regulation of SMARCC2
            ubiquitination
  JOURNAL   Aging (Albany NY) 15 (12), 5381-5398 (2023)
   PUBMED   37348029
  REMARK    GeneRIF: FBXO28 promotes proliferation, invasion, and metastasis of
            pancreatic cancer cells through regulation of SMARCC2
            ubiquitination.
            Erratum:[Aging (Albany NY). 2023 Sep 14;15(17):9230-9232. doi:
            10.18632/aging.205071.. PMID: 37708091]
REFERENCE   5  (bases 1 to 5427)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 5427)
  AUTHORS   D'Angiolella,V., Donato,V., Forrester,F.M., Jeong,Y.T.,
            Pellacani,C., Kudo,Y., Saraf,A., Florens,L., Washburn,M.P. and
            Pagano,M.
  TITLE     Cyclin F-mediated degradation of ribonucleotide reductase M2
            controls genome integrity and DNA repair
  JOURNAL   Cell 149 (5), 1023-1034 (2012)
   PUBMED   22632967
REFERENCE   7  (bases 1 to 5427)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
REFERENCE   8  (bases 1 to 5427)
  AUTHORS   Ohta,S., Bukowski-Wills,J.C., Sanchez-Pulido,L., Alves Fde,L.,
            Wood,L., Chen,Z.A., Platani,M., Fischer,L., Hudson,D.F.,
            Ponting,C.P., Fukagawa,T., Earnshaw,W.C. and Rappsilber,J.
  TITLE     The protein composition of mitotic chromosomes determined using
            multiclassifier combinatorial proteomics
  JOURNAL   Cell 142 (5), 810-821 (2010)
   PUBMED   20813266
REFERENCE   9  (bases 1 to 5427)
  AUTHORS   Jin,J., Cardozo,T., Lovering,R.C., Elledge,S.J., Pagano,M. and
            Harper,J.W.
  TITLE     Systematic analysis and nomenclature of mammalian F-box proteins
  JOURNAL   Genes Dev 18 (21), 2573-2580 (2004)
   PUBMED   15520277
REFERENCE   10 (bases 1 to 5427)
  AUTHORS   Prashar,Y. and Weissman,S.M.
  TITLE     Analysis of differential gene expression by display of 3' end
            restriction fragments of cDNAs
  JOURNAL   Proc Natl Acad Sci U S A 93 (2), 659-663 (1996)
   PUBMED   8570611
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL390122.16.
            
            On Nov 23, 2018 this sequence version replaced NM_015176.3.
            
            Summary: Members of the F-box protein family, such as FBXO28, are
            characterized by an approximately 40-amino acid F-box motif. SCF
            complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM
            603134), and F-box proteins, act as protein-ubiquitin ligases.
            F-box proteins interact with SKP1 through the F box, and they
            interact with ubiquitination targets through other protein
            interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied
            by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK001628.1, SRR1803615.245511.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000366862.10/ ENSP00000355827.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-286               AL390122.16        34118-34403
            287-396             AL390122.16        50479-50588
            397-535             AL390122.16        54081-54219
            536-731             AL390122.16        73149-73344
            732-5427            AL390122.16        77359-82054
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q42.11"
     gene            1..5427
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /note="F-box protein 28"
                     /db_xref="GeneID:23219"
                     /db_xref="HGNC:HGNC:29046"
                     /db_xref="MIM:609100"
     exon            1..286
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..1126
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /note="isoform a is encoded by transcript variant 1; F-box
                     only protein 28; centromere protein 30"
                     /codon_start=1
                     /product="F-box only protein 28 isoform a"
                     /protein_id="NP_055991.1"
                     /db_xref="CCDS:CCDS1539.1"
                     /db_xref="GeneID:23219"
                     /db_xref="HGNC:HGNC:29046"
                     /db_xref="MIM:609100"
                     /translation="MAAAAEERMAEEGGGGQGDGGSSLASGSTQRQPPPPAPQHPQPG
                     SQALPAPALAPDQLPQNNTLVALPIVAIENILSFMSYDEISQLRLVCKRMDLVCQRML
                     NQGFLKVERYHNLCQKQVKAQLPRRESERRNHSLARHADILAAVETRLSLLNMTFMKY
                     VDSNLCCFIPGKVIDEIYRVLRYVNSTRAPQRAHEVLQELRDISSMAMEYFDEKIVPI
                     LKRKLPGSDVSGRLMGSPPVPGPSAALTTMQLFSKQNPSRQEVTKLQQQVKTNGAGVT
                     VLRREISELRTKVQEQQKQLQDQDQKLLEQTQIIGEQNARLAELERKLREVMESAVGN
                     SSGSGQNEESPRKRKKATEAIDSLRKSKRLRNRK"
     exon            287..396
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /inference="alignment:Splign:2.1.0"
     exon            397..535
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /inference="alignment:Splign:2.1.0"
     exon            536..731
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /inference="alignment:Splign:2.1.0"
     exon            732..5427
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2903..2908
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /note="hexamer: AATAAA"
     polyA_site      2923
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /note="major polyA site"
     regulatory      5308..5313
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /note="hexamer: AATAAA"
     polyA_site      5334
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
     regulatory      5406..5411
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /note="hexamer: AATAAA"
     polyA_site      5427
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /note="major polyA site"
ORIGIN      
        1 aaggaatcaa gcccccaaga tggcggcagc ggcggaggag cggatggcag aggaaggagg
       61 cggcggccaa ggcgacggcg gttcctcttt ggcctccggc tctacccagc gacagcctcc
      121 accgcccgcg ccacagcacc cgcagccggg gtcccaggcg ctcccagccc ccgcgctggc
      181 tccggaccag ctgcctcaaa acaacacgct tgtggcgctg cccatcgtag ccatcgagaa
      241 catcctcagc tttatgtcct acgacgaaat tagccagctc cgcctggttt gtaaaagaat
      301 ggacttggtc tgccagagaa tgttgaatca gggatttctg aaagtggaga ggtaccataa
      361 tctatgtcag aaacaagtta aagcacaact cccaaggaga gagtcagaaa ggagaaacca
      421 ttcattagct cgtcatgcag acattcttgc tgctgttgaa acaaggctgt cactattaaa
      481 tatgactttc atgaaatatg tggattccaa tctctgttgc ttcatcccag gaaaggtgat
      541 tgatgagatt tatcgtgtgt tgagatatgt caattctacc agagcccctc aacgagctca
      601 tgaagtactt caagaattaa gggatatatc ctctatggca atggagtact ttgatgaaaa
      661 gattgttcca attttaaaga ggaaattacc aggatcagat gtttctggaa gactcatggg
      721 ctctcctcca gttccaggac cgtctgcagc cctaacaaca atgcagctct tctccaagca
      781 aaatccttca agacaagagg ttaccaaact ccagcagcag gttaaaacaa atggtgctgg
      841 cgtgactgtt ctcaggcgtg aaatttctga gcttcgcacc aaagtgcaag aacagcaaaa
      901 acagcttcaa gaccaggacc agaaactgct agagcagacc cagatcatag gtgaacaaaa
      961 tgcacggttg gcagagctag aacgcaaact acgagaagta atggaaagtg ctgtaggaaa
     1021 ttcctcaggg tccgggcaga atgaggagtc tcctcggaaa cgaaaaaagg ccacggaagc
     1081 catagactct cttaggaaat ctaaacgtct tcggaataga aagtaaattg gaatgtggtt
     1141 ctagttccag aggaagacac agcttagtag cttaagcagt tatgcatatc aggatactgt
     1201 gagcaacatg gtgtccctta agcttctagg ctttcagaac acaacttaaa cttgaactct
     1261 tatggttggg acattctttt cctgcttctc ctgattgaac tgatgcacag aatcttttta
     1321 ctttgcaata gatttgtact aaccagacct gttctatcat gttttgagga gttaactttt
     1381 ttctgtgcag ataatgttga aagtaagtta atttgtttct gcatatatgc acatacatac
     1441 gtaaggatct taaacccagt cttgacagac tagaagttaa gtttaataca gaaaatgtcc
     1501 tgttcacttg aaagaaaaga cctacttttt aaatggacac tatcttggtt cttttttttt
     1561 taagtcatct ttttgttata agtgaccttt gtctggaatg tctgaaaagt agttaatgct
     1621 ttttggtatt gaagtaatgg gtaactaaaa tggacttcca tagtattgac tgtagaagga
     1681 gcctctacaa tattgactat atatttttat aaactactgg caaggaactt acccagctgt
     1741 tatacatgtt ttcagttctc tttgggggcc atgtcctaca tttgcatgga tggatgcatg
     1801 cattgcctag tcaactcact taaaaagctc ttgcactggt attgatcttg aacctctata
     1861 cttctccact ttttagagcg gcgtcttagt ttatttaaac gcttaacatc ttccaccgca
     1921 acagcttgcc tctagagggg gaattgttca tccatttgtc ctccagtttt ataggaacaa
     1981 gagactatta aagcccattg ctttatctgc tctctgtagg gttcaggtac actggctaag
     2041 gcagaacccc aaatttcaag gctctttcta tcaatattgg acctctggga gctaatactc
     2101 ctctttgctc accaccatgt gatcattgat cagatgtggt gagtgagtag gggactcgac
     2161 ttgcgggatg cagttttgcc attgcagcaa agttcattga ggaaaattgt accatgaaaa
     2221 agcattttga ttatttcatt tctggaggat gaccttgagg gaagcttttt aatattagtt
     2281 tcaagctttc cttaccctac aactttaaac aaaagcttta attttgtttg cactcctatt
     2341 ttgagcttgt aactggaaaa gcatgtcttg cactgttcaa cctcctgagt ggtcgcttta
     2401 acaacctcca aattgtgtac agtggttatt ttccccagtt gtatagtttt gagacattca
     2461 attatcactg tcttagttgt attttaattt actaaattat gtagctattt gactgttaag
     2521 ttcttttaaa caactgtttc cttgggcttc agttatttaa agtaaattta ggtgtaatat
     2581 agaaaactgt ccctttccag gtgggagctt aacacctgta taaaacctaa ggttttggta
     2641 agtaccttag ttgaataaaa gcacaaggtt atcacctttt ttatccgtcc accgtgacat
     2701 ggttatgcat cctttagatt aacctcacca aatgaagctt tttctatcca tttttaatat
     2761 tgtccttaca taatattgtc cttagatttt gatcaattct atgtctgact ttgaaattcc
     2821 atttacaatg tagtatgttt tcaatgaaaa accataaagt aacatccaag tgtttcatgg
     2881 tttgttggga aggtaatttt aaaataaaac aatttccaaa aaacatttgt cagccaaggt
     2941 catccataaa agtccccgtc tggaactcgt tttctcagca cttctcattt ttataatcat
     3001 agtatttagc catattagca tatctttaga ggaggcctgg tggaataccc ggggcctcta
     3061 ctagtctgct tttccggtca taggaagact ttttttttta cgtatcaaag catgtgttta
     3121 cagatttaca gagtgtgatc cgtatgcatc aaagggatac agccccagac tgtttcttta
     3181 atggtcattc atgaactatt aaaaatttcc tgaatttctt tttaaactac atctagatag
     3241 cctaaacatc tatgtagtct tccattagtt aacatgtgta tagggctttc ataataagta
     3301 gtcacactgt tacaaggtaa attttctatt acaaaatcca aatttctatt gcatacactt
     3361 ataatcatct attcataaag ctaattcatt gtacttgtta atgcatgaat gttccatgca
     3421 gtaggagaat aaagcactac agttcggttg ttcaaatctt aaatcttagg ctatttagct
     3481 aaaacatgtt aggatattgc cttcaccact tgttaggaca gttatttgat agccattatg
     3541 cttacctatg gatagcataa tggtatttaa ggttcaacaa accgcagatt attatgatca
     3601 gctagaactg acatatgtta caagaaaact gtaaattaac ctcctgagag aaccattctg
     3661 ccctgcattt taagtcaggt atttaaatac tgcgtgttgt taatattgcg ttgtcttagt
     3721 gcaacatttc ttaaagtatt cccgttatct tccttggctg tttttcagag gagacgtctc
     3781 catgttaatg ggtccttccc acatagaaat gtattctggt acagcgctgc acacatggat
     3841 agcaatgtga aaaggcatgc ctaagagaca gaaacggctg accttacccc accccagccg
     3901 gatcctctgc ttatctccca catgcacttg gcttaactgg ttttcacaga ttctgaggtt
     3961 atagaacctg ttgtttttca aactcctgta cagagcctgg aaataatgga gcaaacgcaa
     4021 atgtattgag agtcattata ctttaaaata gttttaaaga ctattggggt accatcaggt
     4081 caaaagccac tgatttggga agcaattttt ttgcaaaaca ctttttttgg agtactgaac
     4141 tttacagggg cttgccttaa tctctattta gtagtttcaa gatatatgcg caattattct
     4201 acgtgttaaa aatgttaaaa tattctatgt agccccaaag gtgggtaaaa cagtataagt
     4261 aatgcctaaa ttaataagct aaaaggtgtc actacagctg gatttttgag agaaaatgga
     4321 cataaaacta ggctatgtgt aataaaaata atggcacctt aagattgcac tattttatgc
     4381 attattttat atgccatttc atagcctgca ctttaatctc caaagaaacg atgtatgatg
     4441 tcccacttgt tccttattct taataaactt tttcctgaga cagggcactt aattctattt
     4501 tactatactg aattagtttc ttggaggtag attcatttct tgagttctct gtttccttag
     4561 ccataatatt ttctaggatc cgggaactcc ctatattgtc tatttccaac attatataat
     4621 tttaatttaa tgtaatgtaa atctcatact tggtgatcag cacattctta tatcctgctc
     4681 tagcaatgag tacccatttg ttacacttac caaactctct aaaaacatat actgtgagta
     4741 agatttgaag ccaggaataa aaggcagtct gaaagaaaac gtcctatacg atgcagttgt
     4801 ttggaaatgg ctgatttctg atatgaaacc caacttgata cactgtttgg ttctttgact
     4861 tgggatctat ctgaattgct ttcactagta cacaaaaggt taagttaaaa ctactttaaa
     4921 cttgtatagc ctgccatctg aggtgaatta ttttcaaaaa atactaaatc tatgaactga
     4981 gactagcaat tagttggctg ctccctacca tgattttact tttaatagca atacagttat
     5041 attggtgata aatatgacag gcttaagtac tgctgtcctt ttgcatcttc ctaagcatct
     5101 tggttaaatt tctgaagttt aaaaattaga tccagatttc ttctgttgtg tctgaagaga
     5161 accatgacat ttagaagtat attggtaatc tattaggtcc attggcaaag tatattggtc
     5221 catattcaaa cctattccaa aaggttagaa gtgtttaaga ttcctttatt gtggtaccat
     5281 gtctgtttac ctatgcttat gagcaacaat aaattactag tttaccttct ggaattttaa
     5341 ttgttataac cgaatcaatt attggaaagt gaaaaacacc tcccggtcac acaacagggt
     5401 acgtaaataa atgtgttgtt accagtg
//
