LOCUS       NM_001136115            5231 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens F-box protein 28 (FBXO28), transcript variant 2, mRNA.
ACCESSION   NM_001136115
VERSION     NM_001136115.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Lee,C.Y., Hubrich,D., Varga,J.K., Schafer,C., Welzel,M.,
            Schumbera,E., Djokic,M., Strom,J.M., Schonfeld,J., Geist,J.L.,
            Polat,F., Gibson,T.J., Keller Valsecchi,C.I., Kumar,M.,
            Schueler-Furman,O. and Luck,K.
  TITLE     Systematic discovery of protein interaction interfaces using
            AlphaFold and experimental validation
  JOURNAL   Mol Syst Biol 20 (2), 75-97 (2024)
   PUBMED   38225382
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Song,G., Sun,Z., Chu,M., Zhang,Z., Chen,J., Wang,Z. and Zhu,X.
  TITLE     FBXO28 promotes cell proliferation, migration and invasion via
            upregulation of the TGF-beta1/SMAD2/3 signaling pathway in ovarian
            cancer
  JOURNAL   BMC Cancer 24 (1), 122 (2024)
   PUBMED   38267923
  REMARK    GeneRIF: FBXO28 promotes cell proliferation, migration and invasion
            via upregulation of the TGF-beta1/SMAD2/3 signaling pathway in
            ovarian cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5231)
  AUTHORS   Qiao,X., Lin,J., Shen,J., Chen,Y., Zheng,L., Ren,H., Zhao,X.,
            Yang,H., Li,P. and Wang,Z.
  TITLE     FBXO28 suppresses liver cancer invasion and metastasis by promoting
            PKA-dependent SNAI2 degradation
  JOURNAL   Oncogene 42 (39), 2878-2891 (2023)
   PUBMED   37596321
  REMARK    GeneRIF: FBXO28 suppresses liver cancer invasion and metastasis by
            promoting PKA-dependent SNAI2 degradation.
REFERENCE   4  (bases 1 to 5231)
  AUTHORS   Liu,S., Liu,P., Zhu,C., Yang,R., He,Z., Li,Y., Li,Y., Fei,X.,
            Hou,J., Wang,X. and Pan,Y.
  TITLE     FBXO28 promotes proliferation, invasion, and metastasis of
            pancreatic cancer cells through regulation of SMARCC2
            ubiquitination
  JOURNAL   Aging (Albany NY) 15 (12), 5381-5398 (2023)
   PUBMED   37348029
  REMARK    GeneRIF: FBXO28 promotes proliferation, invasion, and metastasis of
            pancreatic cancer cells through regulation of SMARCC2
            ubiquitination.
            Erratum:[Aging (Albany NY). 2023 Sep 14;15(17):9230-9232. doi:
            10.18632/aging.205071.. PMID: 37708091]
REFERENCE   5  (bases 1 to 5231)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 5231)
  AUTHORS   D'Angiolella,V., Donato,V., Forrester,F.M., Jeong,Y.T.,
            Pellacani,C., Kudo,Y., Saraf,A., Florens,L., Washburn,M.P. and
            Pagano,M.
  TITLE     Cyclin F-mediated degradation of ribonucleotide reductase M2
            controls genome integrity and DNA repair
  JOURNAL   Cell 149 (5), 1023-1034 (2012)
   PUBMED   22632967
REFERENCE   7  (bases 1 to 5231)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
REFERENCE   8  (bases 1 to 5231)
  AUTHORS   Ohta,S., Bukowski-Wills,J.C., Sanchez-Pulido,L., Alves Fde,L.,
            Wood,L., Chen,Z.A., Platani,M., Fischer,L., Hudson,D.F.,
            Ponting,C.P., Fukagawa,T., Earnshaw,W.C. and Rappsilber,J.
  TITLE     The protein composition of mitotic chromosomes determined using
            multiclassifier combinatorial proteomics
  JOURNAL   Cell 142 (5), 810-821 (2010)
   PUBMED   20813266
REFERENCE   9  (bases 1 to 5231)
  AUTHORS   Jin,J., Cardozo,T., Lovering,R.C., Elledge,S.J., Pagano,M. and
            Harper,J.W.
  TITLE     Systematic analysis and nomenclature of mammalian F-box proteins
  JOURNAL   Genes Dev 18 (21), 2573-2580 (2004)
   PUBMED   15520277
REFERENCE   10 (bases 1 to 5231)
  AUTHORS   Prashar,Y. and Weissman,S.M.
  TITLE     Analysis of differential gene expression by display of 3' end
            restriction fragments of cDNAs
  JOURNAL   Proc Natl Acad Sci U S A 93 (2), 659-663 (1996)
   PUBMED   8570611
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL390122.16.
            
            On May 31, 2019 this sequence version replaced NM_001136115.2.
            
            Summary: Members of the F-box protein family, such as FBXO28, are
            characterized by an approximately 40-amino acid F-box motif. SCF
            complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM
            603134), and F-box proteins, act as protein-ubiquitin ligases.
            F-box proteins interact with SKP1 through the F box, and they
            interact with ubiquitination targets through other protein
            interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied
            by OMIM, Mar 2008].
            
            Transcript Variant: This variant (2) lacks an exon in the 3' coding
            region, which results in a frameshift, compared to variant 1. The
            encoded isoform (b) is shorter and has a distinct C-terminus,
            compared to isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.4260.1, AK307892.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-286               AL390122.16        34118-34403
            287-396             AL390122.16        50479-50588
            397-535             AL390122.16        54081-54219
            536-5231            AL390122.16        77359-82054
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q42.11"
     gene            1..5231
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /note="F-box protein 28"
                     /db_xref="GeneID:23219"
                     /db_xref="HGNC:HGNC:29046"
                     /db_xref="MIM:609100"
     exon            1..286
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..559
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /note="isoform b is encoded by transcript variant 2; F-box
                     only protein 28; centromere protein 30"
                     /codon_start=1
                     /product="F-box only protein 28 isoform b"
                     /protein_id="NP_001129587.1"
                     /db_xref="CCDS:CCDS44320.1"
                     /db_xref="GeneID:23219"
                     /db_xref="HGNC:HGNC:29046"
                     /db_xref="MIM:609100"
                     /translation="MAAAAEERMAEEGGGGQGDGGSSLASGSTQRQPPPPAPQHPQPG
                     SQALPAPALAPDQLPQNNTLVALPIVAIENILSFMSYDEISQLRLVCKRMDLVCQRML
                     NQGFLKVERYHNLCQKQVKAQLPRRESERRNHSLARHADILAAVETRLSLLNMTFMKY
                     VDSNLCCFIPGKFQDRLQP"
     exon            287..396
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /inference="alignment:Splign:2.1.0"
     exon            397..535
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /inference="alignment:Splign:2.1.0"
     exon            536..5231
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2707..2712
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /note="hexamer: AATAAA"
     polyA_site      2727
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /note="major polyA site"
     regulatory      5112..5117
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /note="hexamer: AATAAA"
     polyA_site      5138
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
     regulatory      5210..5215
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /note="hexamer: AATAAA"
     polyA_site      5231
                     /gene="FBXO28"
                     /gene_synonym="CENP-30; DEE100; Fbx28"
                     /note="major polyA site"
ORIGIN      
        1 aaggaatcaa gcccccaaga tggcggcagc ggcggaggag cggatggcag aggaaggagg
       61 cggcggccaa ggcgacggcg gttcctcttt ggcctccggc tctacccagc gacagcctcc
      121 accgcccgcg ccacagcacc cgcagccggg gtcccaggcg ctcccagccc ccgcgctggc
      181 tccggaccag ctgcctcaaa acaacacgct tgtggcgctg cccatcgtag ccatcgagaa
      241 catcctcagc tttatgtcct acgacgaaat tagccagctc cgcctggttt gtaaaagaat
      301 ggacttggtc tgccagagaa tgttgaatca gggatttctg aaagtggaga ggtaccataa
      361 tctatgtcag aaacaagtta aagcacaact cccaaggaga gagtcagaaa ggagaaacca
      421 ttcattagct cgtcatgcag acattcttgc tgctgttgaa acaaggctgt cactattaaa
      481 tatgactttc atgaaatatg tggattccaa tctctgttgc ttcatcccag gaaagttcca
      541 ggaccgtctg cagccctaac aacaatgcag ctcttctcca agcaaaatcc ttcaagacaa
      601 gaggttacca aactccagca gcaggttaaa acaaatggtg ctggcgtgac tgttctcagg
      661 cgtgaaattt ctgagcttcg caccaaagtg caagaacagc aaaaacagct tcaagaccag
      721 gaccagaaac tgctagagca gacccagatc ataggtgaac aaaatgcacg gttggcagag
      781 ctagaacgca aactacgaga agtaatggaa agtgctgtag gaaattcctc agggtccggg
      841 cagaatgagg agtctcctcg gaaacgaaaa aaggccacgg aagccataga ctctcttagg
      901 aaatctaaac gtcttcggaa tagaaagtaa attggaatgt ggttctagtt ccagaggaag
      961 acacagctta gtagcttaag cagttatgca tatcaggata ctgtgagcaa catggtgtcc
     1021 cttaagcttc taggctttca gaacacaact taaacttgaa ctcttatggt tgggacattc
     1081 ttttcctgct tctcctgatt gaactgatgc acagaatctt tttactttgc aatagatttg
     1141 tactaaccag acctgttcta tcatgttttg aggagttaac ttttttctgt gcagataatg
     1201 ttgaaagtaa gttaatttgt ttctgcatat atgcacatac atacgtaagg atcttaaacc
     1261 cagtcttgac agactagaag ttaagtttaa tacagaaaat gtcctgttca cttgaaagaa
     1321 aagacctact ttttaaatgg acactatctt ggttcttttt tttttaagtc atctttttgt
     1381 tataagtgac ctttgtctgg aatgtctgaa aagtagttaa tgctttttgg tattgaagta
     1441 atgggtaact aaaatggact tccatagtat tgactgtaga aggagcctct acaatattga
     1501 ctatatattt ttataaacta ctggcaagga acttacccag ctgttataca tgttttcagt
     1561 tctctttggg ggccatgtcc tacatttgca tggatggatg catgcattgc ctagtcaact
     1621 cacttaaaaa gctcttgcac tggtattgat cttgaacctc tatacttctc cactttttag
     1681 agcggcgtct tagtttattt aaacgcttaa catcttccac cgcaacagct tgcctctaga
     1741 gggggaattg ttcatccatt tgtcctccag ttttatagga acaagagact attaaagccc
     1801 attgctttat ctgctctctg tagggttcag gtacactggc taaggcagaa ccccaaattt
     1861 caaggctctt tctatcaata ttggacctct gggagctaat actcctcttt gctcaccacc
     1921 atgtgatcat tgatcagatg tggtgagtga gtaggggact cgacttgcgg gatgcagttt
     1981 tgccattgca gcaaagttca ttgaggaaaa ttgtaccatg aaaaagcatt ttgattattt
     2041 catttctgga ggatgacctt gagggaagct ttttaatatt agtttcaagc tttccttacc
     2101 ctacaacttt aaacaaaagc tttaattttg tttgcactcc tattttgagc ttgtaactgg
     2161 aaaagcatgt cttgcactgt tcaacctcct gagtggtcgc tttaacaacc tccaaattgt
     2221 gtacagtggt tattttcccc agttgtatag ttttgagaca ttcaattatc actgtcttag
     2281 ttgtatttta atttactaaa ttatgtagct atttgactgt taagttcttt taaacaactg
     2341 tttccttggg cttcagttat ttaaagtaaa tttaggtgta atatagaaaa ctgtcccttt
     2401 ccaggtggga gcttaacacc tgtataaaac ctaaggtttt ggtaagtacc ttagttgaat
     2461 aaaagcacaa ggttatcacc ttttttatcc gtccaccgtg acatggttat gcatccttta
     2521 gattaacctc accaaatgaa gctttttcta tccattttta atattgtcct tacataatat
     2581 tgtccttaga ttttgatcaa ttctatgtct gactttgaaa ttccatttac aatgtagtat
     2641 gttttcaatg aaaaaccata aagtaacatc caagtgtttc atggtttgtt gggaaggtaa
     2701 ttttaaaata aaacaatttc caaaaaacat ttgtcagcca aggtcatcca taaaagtccc
     2761 cgtctggaac tcgttttctc agcacttctc atttttataa tcatagtatt tagccatatt
     2821 agcatatctt tagaggaggc ctggtggaat acccggggcc tctactagtc tgcttttccg
     2881 gtcataggaa gacttttttt tttacgtatc aaagcatgtg tttacagatt tacagagtgt
     2941 gatccgtatg catcaaaggg atacagcccc agactgtttc tttaatggtc attcatgaac
     3001 tattaaaaat ttcctgaatt tctttttaaa ctacatctag atagcctaaa catctatgta
     3061 gtcttccatt agttaacatg tgtatagggc tttcataata agtagtcaca ctgttacaag
     3121 gtaaattttc tattacaaaa tccaaatttc tattgcatac acttataatc atctattcat
     3181 aaagctaatt cattgtactt gttaatgcat gaatgttcca tgcagtagga gaataaagca
     3241 ctacagttcg gttgttcaaa tcttaaatct taggctattt agctaaaaca tgttaggata
     3301 ttgccttcac cacttgttag gacagttatt tgatagccat tatgcttacc tatggatagc
     3361 ataatggtat ttaaggttca acaaaccgca gattattatg atcagctaga actgacatat
     3421 gttacaagaa aactgtaaat taacctcctg agagaaccat tctgccctgc attttaagtc
     3481 aggtatttaa atactgcgtg ttgttaatat tgcgttgtct tagtgcaaca tttcttaaag
     3541 tattcccgtt atcttccttg gctgtttttc agaggagacg tctccatgtt aatgggtcct
     3601 tcccacatag aaatgtattc tggtacagcg ctgcacacat ggatagcaat gtgaaaaggc
     3661 atgcctaaga gacagaaacg gctgacctta ccccacccca gccggatcct ctgcttatct
     3721 cccacatgca cttggcttaa ctggttttca cagattctga ggttatagaa cctgttgttt
     3781 ttcaaactcc tgtacagagc ctggaaataa tggagcaaac gcaaatgtat tgagagtcat
     3841 tatactttaa aatagtttta aagactattg gggtaccatc aggtcaaaag ccactgattt
     3901 gggaagcaat ttttttgcaa aacacttttt ttggagtact gaactttaca ggggcttgcc
     3961 ttaatctcta tttagtagtt tcaagatata tgcgcaatta ttctacgtgt taaaaatgtt
     4021 aaaatattct atgtagcccc aaaggtgggt aaaacagtat aagtaatgcc taaattaata
     4081 agctaaaagg tgtcactaca gctggatttt tgagagaaaa tggacataaa actaggctat
     4141 gtgtaataaa aataatggca ccttaagatt gcactatttt atgcattatt ttatatgcca
     4201 tttcatagcc tgcactttaa tctccaaaga aacgatgtat gatgtcccac ttgttcctta
     4261 ttcttaataa actttttcct gagacagggc acttaattct attttactat actgaattag
     4321 tttcttggag gtagattcat ttcttgagtt ctctgtttcc ttagccataa tattttctag
     4381 gatccgggaa ctccctatat tgtctatttc caacattata taattttaat ttaatgtaat
     4441 gtaaatctca tacttggtga tcagcacatt cttatatcct gctctagcaa tgagtaccca
     4501 tttgttacac ttaccaaact ctctaaaaac atatactgtg agtaagattt gaagccagga
     4561 ataaaaggca gtctgaaaga aaacgtccta tacgatgcag ttgtttggaa atggctgatt
     4621 tctgatatga aacccaactt gatacactgt ttggttcttt gacttgggat ctatctgaat
     4681 tgctttcact agtacacaaa aggttaagtt aaaactactt taaacttgta tagcctgcca
     4741 tctgaggtga attattttca aaaaatacta aatctatgaa ctgagactag caattagttg
     4801 gctgctccct accatgattt tacttttaat agcaatacag ttatattggt gataaatatg
     4861 acaggcttaa gtactgctgt ccttttgcat cttcctaagc atcttggtta aatttctgaa
     4921 gtttaaaaat tagatccaga tttcttctgt tgtgtctgaa gagaaccatg acatttagaa
     4981 gtatattggt aatctattag gtccattggc aaagtatatt ggtccatatt caaacctatt
     5041 ccaaaaggtt agaagtgttt aagattcctt tattgtggta ccatgtctgt ttacctatgc
     5101 ttatgagcaa caataaatta ctagtttacc ttctggaatt ttaattgtta taaccgaatc
     5161 aattattgga aagtgaaaaa cacctcccgg tcacacaaca gggtacgtaa ataaatgtgt
     5221 tgttaccagt g
//
