LOCUS       NM_001258211            1218 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens F-box protein 16 (FBXO16), transcript variant 2, mRNA.
ACCESSION   NM_001258211
VERSION     NM_001258211.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Zhang,Z., Liu,X., Chu,C., Zhang,Y., Li,W., Yu,X., Han,Q., Sun,H.,
            Zhang,Y., Zhu,X., Chen,L., Wei,R., Fan,N., Zhou,M. and Li,X.
  TITLE     MIR937 amplification potentiates ovarian cancer progression by
            attenuating FBXO16 inhibition on ULK1-mediated autophagy
  JOURNAL   Cell Death Dis 15 (10), 735 (2024)
   PUBMED   39384743
  REMARK    GeneRIF: MIR937 amplification potentiates ovarian cancer
            progression by attenuating FBXO16 inhibition on ULK1-mediated
            autophagy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Ji,M., Zhao,Z., Li,Y., Xu,P., Shi,J., Li,Z., Wang,K., Huang,X.,
            Ji,J., Liu,W. and Liu,B.
  TITLE     FBXO16-mediated hnRNPL ubiquitination and degradation plays a tumor
            suppressor role in ovarian cancer
  JOURNAL   Cell Death Dis 12 (8), 758 (2021)
   PUBMED   34333526
  REMARK    GeneRIF: FBXO16-mediated hnRNPL ubiquitination and degradation
            plays a tumor suppressor role in ovarian cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1218)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1218)
  AUTHORS   Paul,D., Islam,S., Manne,R.K., Dinesh,U.S., Malonia,S.K., Maity,B.,
            Boppana,R., Rapole,S., Shetty,P.K. and Santra,M.K.
  TITLE     F-box protein FBXO16 functions as a tumor suppressor by attenuating
            nuclear beta-catenin function
  JOURNAL   J Pathol 248 (3), 266-279 (2019)
   PUBMED   30714168
  REMARK    GeneRIF: that FBXO16 functions as a putative tumor suppressor by
            forming an SCF(FBXO16) complex that targets nuclear beta-catenin in
            a unique manner for ubiquitination and subsequent proteasomal
            degradation to prevent malignancy
REFERENCE   5  (bases 1 to 1218)
  AUTHORS   Khan,M., Muzumdar,D. and Shiras,A.
  TITLE     Attenuation of Tumor Suppressive Function of FBXO16 Ubiquitin
            Ligase Activates Wnt Signaling In Glioblastoma
  JOURNAL   Neoplasia 21 (1), 106-116 (2019)
   PUBMED   30530053
  REMARK    GeneRIF: we show that due to low expression of FBXO16, the
            beta-catenin is not targeted in glioma cells leading to its nuclear
            accumulation resulting in active Wnt signaling. Activated Wnt
            signaling potentiates the glioma cells toward a highly
            proliferative and malignant state.
REFERENCE   6  (bases 1 to 1218)
  AUTHORS   Wang,A.G., Yoon,S.Y., Oh,J.H., Jeon,Y.J., Kim,M., Kim,J.M.,
            Byun,S.S., Yang,J.O., Kim,J.H., Kim,D.G., Yeom,Y.I., Yoo,H.S.,
            Kim,Y.S. and Kim,N.S.
  TITLE     Identification of intrahepatic cholangiocarcinoma related genes by
            comparison with normal liver tissues using expressed sequence tags
  JOURNAL   Biochem Biophys Res Commun 345 (3), 1022-1032 (2006)
   PUBMED   16712791
REFERENCE   7  (bases 1 to 1218)
  AUTHORS   Cheng,H., Ma,Y., Ni,X., Jiang,M., Guo,L., Jin,W., Xu,W., Cao,G.,
            Ji,C., Yin,K., Gu,S., Ma,Y., Xie,Y. and Mao,Y.
  TITLE     cDNA cloning and expression analysis of a novel human F-box only
            protein
  JOURNAL   Mol Cells 14 (1), 56-59 (2002)
   PUBMED   12243353
  REMARK    GeneRIF: FBXO16 was localized to the human chromosome 8p12. The
            FBXO16 gene consisted of 9 exons that spanned 67,816 bp of human
            genomic DNA
REFERENCE   8  (bases 1 to 1218)
  AUTHORS   Cenciarelli,C., Chiaur,D.S., Guardavaccaro,D., Parks,W., Vidal,M.
            and Pagano,M.
  TITLE     Identification of a family of human F-box proteins
  JOURNAL   Curr Biol 9 (20), 1177-1179 (1999)
   PUBMED   10531035
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC102028.1, AC025871.8 and
            AA765256.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001258211.1.
            
            Summary: This gene encodes a member of the F-box protein family,
            members of which are characterized by an approximately 40 amino
            acid motif, the F-box. The F-box proteins constitute one of the
            four subunits of ubiquitin protein ligase complex called SCFs
            (SKP1-cullin-F-box), which function in phosphorylation-dependent
            ubiquitination. The F-box proteins are divided into three classes:
            Fbws containing WD-40 domains, Fbls containing leucine-rich
            repeats, and Fbxs containing either different protein-protein
            interaction modules or no recognizable motifs. The protein encoded
            by this gene belongs to the Fbx class. Multiple transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Apr 2012].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting protein (isoform 2) is
            shorter compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC102028.1, SRR14372080.467915.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-987               BC102028.1         9-995
            988-1186            AC025871.8         62039-62237
            1187-1218           AA765256.1         44-75               c
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p21.1"
     gene            1..1218
                     /gene="FBXO16"
                     /gene_synonym="FBX16"
                     /note="F-box protein 16"
                     /db_xref="GeneID:157574"
                     /db_xref="HGNC:HGNC:13618"
                     /db_xref="MIM:608519"
     exon            1..44
                     /gene="FBXO16"
                     /gene_synonym="FBX16"
                     /inference="alignment:Splign:2.1.0"
     exon            45..159
                     /gene="FBXO16"
                     /gene_synonym="FBX16"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..903
                     /gene="FBXO16"
                     /gene_synonym="FBX16"
                     /note="isoform 2 is encoded by transcript variant 2; F-box
                     only protein 16"
                     /codon_start=1
                     /product="F-box only protein 16 isoform 2"
                     /protein_id="NP_001245140.1"
                     /db_xref="CCDS:CCDS59099.1"
                     /db_xref="GeneID:157574"
                     /db_xref="HGNC:HGNC:13618"
                     /db_xref="MIM:608519"
                     /translation="MMAFAPPKNTDGPKMQTKMSTWTPLNHQLLNDRFDKWTDSQRRR
                     ILTGLLERCSLSQQKFCCRKLQEKIPAEALDFTTKLPRVLSLYIFSFLDPRSLCRCAQ
                     VCWHWKNLAELDQLWMLKCLRFNWYINFSPTPFEQGIWKKHYIQMVKELHITKPKTPP
                     KDGFVIADVQLVTSNSPEEKQSPLSAFRSSSSLRKKNNSGEKALPPWRSSDKHPTDII
                     RFNYLDNRDPMETVQQGRRKRNQMTPDFSRQSHDKKNKLQDRTRLRKAQSMMSRRNPF
                     PLCP"
     exon            160..366
                     /gene="FBXO16"
                     /gene_synonym="FBX16"
                     /inference="alignment:Splign:2.1.0"
     exon            367..531
                     /gene="FBXO16"
                     /gene_synonym="FBX16"
                     /inference="alignment:Splign:2.1.0"
     exon            532..764
                     /gene="FBXO16"
                     /gene_synonym="FBX16"
                     /inference="alignment:Splign:2.1.0"
     exon            765..867
                     /gene="FBXO16"
                     /gene_synonym="FBX16"
                     /inference="alignment:Splign:2.1.0"
     exon            868..893
                     /gene="FBXO16"
                     /gene_synonym="FBX16"
                     /inference="alignment:Splign:2.1.0"
     exon            894..1218
                     /gene="FBXO16"
                     /gene_synonym="FBX16"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1198..1203
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FBXO16"
                     /gene_synonym="FBX16"
                     /note="hexamer: AGTAAA"
     polyA_site      1218
                     /gene="FBXO16"
                     /gene_synonym="FBX16"
                     /note="major polyA site"
ORIGIN      
        1 aacgtcagcg cttgaggctt gaagagggag acaagctaaa agaggatatc cagttgtttc
       61 atgatggcat ttgcacctcc aaaaaacaca gatggtccca aaatgcagac aaagatgagc
      121 acctggacac ccctaaacca tcagctattg aatgaccggt ttgacaaatg gacagactct
      181 caaagaagaa gaatcctcac aggcctgttg gagcgctgct cgctgtccca gcaaaagttc
      241 tgctgtcgaa agcttcaaga gaaaattcca gcagaagccc tggactttac aaccaagctt
      301 ccaagggtgt tatctttata catcttttct ttcctggacc ctcggagcct ttgtcgttgt
      361 gcacaggtgt gctggcattg gaagaacctt gctgagctgg accagctctg gatgctgaaa
      421 tgtttacggt ttaactggta catcaatttc tctccaactc cctttgagca ggggatctgg
      481 aagaagcact atattcaaat ggtgaaagaa cttcatatta ccaagcctaa gacaccccca
      541 aaggatggat ttgtaatcgc tgacgttcaa ctagttacaa gcaattctcc agaggaaaaa
      601 cagtcccctt tatcagcttt tcggtcctct tcctctttaa gaaagaagaa taactcaggg
      661 gagaaagcac ttccaccctg gcgatcttct gataagcacc caacagatat cattcgtttt
      721 aattacctag acaaccgtga ccccatggag actgtccagc aaggaagaag aaaaagaaac
      781 caaatgaccc cagacttcag ccgacagtca catgataaga aaaataaatt gcaggacaga
      841 actaggctaa gaaaagcaca atcaatgatg tcgaggagaa atcccttccc actatgtccc
      901 taagtgccag ctctccccta aaagttccag ctcatctcgc ctggcctccc cctgagtcag
      961 tgggactccc agacactgcc accacagctg aaattctcat gcagcatcct cacaggcacc
     1021 ctgggcccca agcatgactc atccaggttc cagagccaaa gtggactgaa catggaaaga
     1081 cttttattat agaaatgaca agatgctttg cacagtggag agctgaattt acttggctcc
     1141 cattagaaac tctttcagct taagtactta ttgtggtagt gagtcctacg gtatttcagt
     1201 aaaaaggaat tcatggca
//
