LOCUS       NM_001193534            3485 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens F-box and leucine rich repeat protein 5 (FBXL5),
            transcript variant 3, mRNA.
ACCESSION   NM_001193534
VERSION     NM_001193534.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Zhang,S., Ji,B., Li,J., Ji,W., Yang,C. and Yang,L.
  TITLE     FBXL5 promotes lipid accumulation in alcoholic fatty liver disease
            by promoting the ubiquitination and degradation of TFEB
  JOURNAL   Cell Signal 112, 110905 (2023)
   PUBMED   37743009
  REMARK    GeneRIF: FBXL5 promotes lipid accumulation in alcoholic fatty liver
            disease by promoting the ubiquitination and degradation of TFEB.
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Cho,Y.A., Kim,S.E., Park,C.K., Koh,H.H., Park,C.K. and Ha,S.Y.
  TITLE     Loss of F-Box and Leucine Rich Repeat Protein 5 (FBXL5) Expression
            Is Associated With Poor Survival in Patients With Hepatocellular
            Carcinoma After Curative Resection: A Two-institute Study
  JOURNAL   Cancer Genomics Proteomics 20 (3), 298-307 (2023)
   PUBMED   37093682
  REMARK    GeneRIF: Loss of F-Box and Leucine Rich Repeat Protein 5 (FBXL5)
            Expression Is Associated With Poor Survival in Patients With
            Hepatocellular Carcinoma After Curative Resection: A Two-institute
            Study.
REFERENCE   3  (bases 1 to 3485)
  AUTHORS   Park,C.K., Heo,J., Ham,W.S., Choi,Y.D., Shin,S.J. and Cho,N.H.
  TITLE     Ferroportin and FBXL5 as Prognostic Markers in Advanced Stage Clear
            Cell Renal Cell Carcinoma
  JOURNAL   Cancer Res Treat 53 (4), 1174-1183 (2021)
   PUBMED   33735560
  REMARK    GeneRIF: Ferroportin and FBXL5 as Prognostic Markers in Advanced
            Stage Clear Cell Renal Cell Carcinoma.
REFERENCE   4  (bases 1 to 3485)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 3485)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 3485)
  AUTHORS   Ebstein,F., Lange,N., Urban,S., Seifert,U., Kruger,E. and
            Kloetzel,P.M.
  TITLE     Maturation of human dendritic cells is accompanied by functional
            remodelling of the ubiquitin-proteasome system
  JOURNAL   Int J Biochem Cell Biol 41 (5), 1205-1215 (2009)
   PUBMED   19028597
REFERENCE   7  (bases 1 to 3485)
  AUTHORS   Zhang,N., Liu,J., Ding,X., Aikhionbare,F., Jin,C. and Yao,X.
  TITLE     FBXL5 interacts with p150Glued and regulates its ubiquitination
  JOURNAL   Biochem Biophys Res Commun 359 (1), 34-39 (2007)
   PUBMED   17532294
  REMARK    GeneRIF: Our findings provide a potential mechanism by which
            p150(Glued) protein function is regulated by SCFs.
REFERENCE   8  (bases 1 to 3485)
  AUTHORS   Ilyin,G.P., Rialland,M., Pigeon,C. and Guguen-Guillouzo,C.
  TITLE     cDNA cloning and expression analysis of new members of the
            mammalian F-box protein family
  JOURNAL   Genomics 67 (1), 40-47 (2000)
   PUBMED   10945468
REFERENCE   9  (bases 1 to 3485)
  AUTHORS   Winston,J.T., Koepp,D.M., Zhu,C., Elledge,S.J. and Harper,J.W.
  TITLE     A family of mammalian F-box proteins
  JOURNAL   Curr Biol 9 (20), 1180-1182 (1999)
   PUBMED   10531037
REFERENCE   10 (bases 1 to 3485)
  AUTHORS   Cenciarelli,C., Chiaur,D.S., Guardavaccaro,D., Parks,W., Vidal,M.
            and Pagano,M.
  TITLE     Identification of a family of human F-box proteins
  JOURNAL   Curr Biol 9 (20), 1177-1179 (1999)
   PUBMED   10531035
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC030656.1, AF199420.1,
            AF142481.1, BF432189.1, AI858616.1 and AC116651.6.
            
            On Aug 13, 2020 this sequence version replaced NM_001193534.1.
            
            Summary: This gene encodes a member of the F-box protein family
            which is characterized by an approximately 40 amino acid motif, the
            F-box. The F-box proteins constitute one of the four subunits of
            ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box),
            which function in phosphorylation-dependent ubiquitination. The
            F-box proteins are divided into 3 classes: Fbws containing WD-40
            domains, Fbls containing leucine-rich repeats, and Fbxs containing
            either different protein-protein interaction modules or no
            recognizable motifs. The protein encoded by this gene belongs to
            the Fbls class and, in addition to an F-box, contains several
            tandem leucine-rich repeats. Alternatively spliced transcript
            variants have been described for this locus. [provided by RefSeq,
            Aug 2010].
            
            Transcript Variant: This variant (3) uses an alternate in-frame
            splice site in the 5' coding region, compared to variant 1. This
            results in a shorter protein (isoform 3), compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF199420.1, SRR14038195.283529.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-62                BC030656.1         27-88
            63-2143             AF199420.1         7-2087
            2144-2852           AF142481.1         2124-2832
            2853-3327           BF432189.1         3-477               c
            3328-3335           AI858616.1         147-154             c
            3336-3336           AC116651.6         42381-42381         c
            3337-3482           AI858616.1         1-146               c
            3483-3485           AC116651.6         42232-42234         c
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p15.32"
     gene            1..3485
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /note="F-box and leucine rich repeat protein 5"
                     /db_xref="GeneID:26234"
                     /db_xref="HGNC:HGNC:13602"
                     /db_xref="MIM:605655"
     exon            1..154
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..2143
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /note="isoform 3 is encoded by transcript variant 3;
                     F-box/LRR-repeat protein 5; p45SKP2-like protein; F-box
                     protein FBL4/FBL5"
                     /codon_start=1
                     /product="F-box/LRR-repeat protein 5 isoform 3"
                     /protein_id="NP_001180463.1"
                     /db_xref="GeneID:26234"
                     /db_xref="HGNC:HGNC:13602"
                     /db_xref="MIM:605655"
                     /translation="MAPFPEEVDVFTAPHWRMKQLVGLYCDKLSKTNFSNNNDFRALL
                     QSLYATFKEFKMHEQIENEYIIGLLQQRSQTIYNVHSDNKLSEMLSLFEKGLKNVKNE
                     YEQLNYAKQLKERLEAFTRDFLPHMKEEEEVFQPMLMEYFTYEELKDIKKKVIAQHCS
                     QKDTAELLRGLSLWNHAEERQKFFKYSVDEKSDKAEVSEHSTGITHLPPEVMLSIFSY
                     LNPQELCRCSQVSMKWSQLTKTGSLWKHLYPVHWARGDWYSGPATELDTEPDDEWVKN
                     RKDESRAFHEWDEDADIDESEESAEESIAISIAQMEKRLLHGLIHNVLPYVGTSVKTL
                     VLAYSSAVSSKMVRQILELCPNLEHLDLTQTDISDSAFDSWSWLGCCQSLRHLDLSGC
                     EKITDVALEKISRALGILTSHQSGFLKTSTSKITSTAWKNKDITMQSTKQYACLHDLT
                     NKGIGEEIDNEHPWTKPVSSENFTSPYVWMLDAEDLADIEDTVEWRHRNVESLCVMET
                     ASNFSCSTSGCFSKDIVGLRTSVCWQQHCASPAFAYCGHSFCCTGTALRTMSSLPESS
                     AMCRKAARTRLPRGKDLIYFGSEKSDQETGRVLLFLSLSGCYQITDHGLRVLTLGGGL
                     PYLEHLNLSGCLTITGAGLQDLVSACPSLNDEYFYYCDNINGPHADTASGCQNLQCGF
                     RACCRSGE"
     exon            155..370
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /inference="alignment:Splign:2.1.0"
     exon            371..466
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /inference="alignment:Splign:2.1.0"
     exon            467..653
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /inference="alignment:Splign:2.1.0"
     exon            654..833
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /inference="alignment:Splign:2.1.0"
     exon            834..959
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1108
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1191
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1917
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2066
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..3485
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2828..2833
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /note="hexamer: AATAAA"
     polyA_site      2860
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /note="major polyA site"
     regulatory      3309..3314
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /note="hexamer: AATAAA"
     polyA_site      3336
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
     regulatory      3379..3384
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /note="hexamer: ATTAAA"
     polyA_site      3397
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
     regulatory      3459..3464
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
                     /note="hexamer: AATAAG"
     polyA_site      3485
                     /gene="FBXL5"
                     /gene_synonym="FBL4; FBL5; FLR1"
ORIGIN      
        1 gccccgcggc gagggtgtct atggagaggc ggcggccgcg gctgctgagg cggaggctga
       61 ggcagtggcg atggcgccct ttcctgaaga agtggacgtc ttcaccgccc cacactggcg
      121 gatgaagcag ctggtggggc tctactgcga caagctttct aaaaccaatt tttccaacaa
      181 caacgatttc cgtgctcttc tgcagtcttt gtatgctact ttcaaggagt tcaaaatgca
      241 tgagcagatt gaaaatgaat acattattgg tttgcttcaa caacgcagcc agaccattta
      301 taatgtacat tctgacaata aactctccga gatgcttagc ctctttgaaa agggactgaa
      361 gaatgttaag aatgaatatg aacagttaaa ttatgcaaaa caactgaaag agagattgga
      421 ggcttttaca agagattttc ttcctcacat gaaagaggaa gaggaggttt ttcagcccat
      481 gttaatggaa tattttacct atgaagagct taaggatatt aaaaagaaag tgattgcaca
      541 acactgctct cagaaggata ctgcagaact ccttagaggt cttagcctat ggaatcatgc
      601 tgaagagcga cagaagtttt ttaaatattc cgtggatgaa aagtcagata aagcagaagt
      661 gtcagaacac tccacaggta taacccatct tcctcctgag gtaatgctgt caattttcag
      721 ctatcttaat cctcaagagt tatgtcgatg cagtcaagta agcatgaaat ggtctcagct
      781 gacaaaaacg ggatcgcttt ggaaacatct ttaccctgtt cattgggcca gaggtgactg
      841 gtatagtggt cccgcaactg aacttgatac tgaacctgat gatgaatggg tgaaaaatag
      901 gaaagatgaa agtcgtgctt ttcatgagtg ggatgaagat gctgacattg atgaatctga
      961 agagtctgcg gaggaatcaa ttgctatcag cattgcacaa atggaaaaac gtttactcca
     1021 tggcttaatt cataacgttc taccatatgt tggtacttct gtaaaaacct tagtattagc
     1081 atacagctct gcagtttcca gcaaaatggt taggcagatt ttagagcttt gtcctaacct
     1141 ggagcatctg gatcttaccc agactgacat ttcagattct gcatttgaca gttggtcttg
     1201 gcttggttgc tgccagagtc ttcggcatct tgatctgtct ggttgtgaga aaatcacaga
     1261 tgtggcccta gagaagattt ccagagctct tggaattctg acatctcatc aaagtggctt
     1321 tttgaaaaca tctacaagca aaattacttc aactgcgtgg aaaaataaag acattaccat
     1381 gcagtccacc aagcagtatg cctgtttgca cgatttaact aacaagggca ttggagaaga
     1441 aatagataat gaacacccct ggactaagcc tgtttcttct gagaatttca cttctcctta
     1501 tgtgtggatg ttagatgctg aagatttggc tgatattgaa gatactgtgg aatggagaca
     1561 tagaaatgtt gaaagtcttt gtgtaatgga aacagcatcc aactttagtt gttccacctc
     1621 tggttgtttt agtaaggaca ttgttggact aaggactagt gtctgttggc agcagcattg
     1681 tgcttctcca gcctttgcgt attgtggtca ctcattttgt tgtacaggaa cagctttaag
     1741 aactatgtca tcactcccag aatcttctgc aatgtgtaga aaagcagcaa ggactagatt
     1801 gcctagggga aaagacttaa tttactttgg gagtgaaaaa tctgatcaag agactggacg
     1861 tgtacttctg tttctcagtt tatctggatg ttatcagatc acagaccatg gtctcagggt
     1921 tttgactctg ggaggagggc tgccttattt ggagcacctt aatctctctg gttgtcttac
     1981 tataactggt gcaggcctgc aggatttggt ttcagcatgt ccttctctga atgatgaata
     2041 cttttactac tgtgacaaca ttaacggtcc tcatgctgat accgccagtg gatgccagaa
     2101 tttgcagtgt ggttttcgag cctgctgccg ctctggcgaa tgacccttga cttctgatct
     2161 ttgtctactt catttagctg agcaggcttt ctttcatgca ctttactcat agcacatttc
     2221 ttgtgttaac catccctttt tgagcgtgac ttgttttggc cccatttctt acaacttcag
     2281 aaatcttaat ttaccagtga attgtaatgt tgtttctctt gcaaattata cttttggttt
     2341 agaaagggat taggtctttt caaaagggtg agaacagtct tacatttttc ttttaaatga
     2401 aatgctttaa agaatgttgg taatgccatg tcatttaaag tatttcatag ataattttga
     2461 gttttaaagt ccatggaggt gattggttct ctttacacat taacactgta ccaagctttg
     2521 cagatctttt ccgacacaca tgtctgaaga cttattttca aagacagcac atttttggaa
     2581 actaatctct tttccgtaat atttccttta tttcaatgat tctcagaagg ccaattcaaa
     2641 caaacccaca tttaaggttc tttaggatta tagaataaat tggcttctga gtgttagctc
     2701 agtgagctag gaaagcacca atcgatattt gtttccttta gggatacttt gttctcacca
     2761 ctgtccctat gtcatcaaat ttgggagaga ttttttaaaa taccacaatc atttgaagaa
     2821 atgtataaat aaaatctact ttgaggactt taccaagtaa tactgttgtg ttgtgatttt
     2881 tattataaat ctcaatccag gtttacttta gtatagaata ggactacata ctttgtcttt
     2941 ggacctccca tgaacttatt accctgacaa atagttttag acagatactg aattttattg
     3001 agctgaattt tctttttgaa atgagttatt cagtagtcaa aatttgaatc cgtaagtata
     3061 tctactttgc tttgttaaaa catcatgaga gtggaggcct gccacccaga aaggcacata
     3121 ctagtgcctt ttagtacaat tttatgtcat gtagaagaaa atggcaatgt tcctcctatt
     3181 gatacagata caggcatatg tcatcttatt gcgttttgct ttattgcact ttgcaaataa
     3241 tgcatttttt acaaatggaa ggtttatggc aactctgcgt tgagccagtc tattggtgcc
     3301 atgtttccaa taaaacttct cctttcatgt ctcgtatatt gtgataattc ttggagtatt
     3361 tcatagtttt tcactattat taaatctgtt gtggtgatct gtggccagtg attgttgtta
     3421 ctattgtaat tgctttggaa tgccatgaac tgtgccctaa taagacagag aacttaattg
     3481 ataaa
//
