LOCUS       NM_032875              10332 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens F-box and leucine rich repeat protein 20 (FBXL20),
            transcript variant 1, mRNA.
ACCESSION   NM_032875 NR_026953
VERSION     NM_032875.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10332)
  AUTHORS   Liu,J., Zhan,W., Chen,G., Yan,S., Chen,W. and Li,R.
  TITLE     SP1-induced PROX1-AS1 contributes to tumor progression by
            regulating miR-326/FBXL20 axis in colorectal cancer
  JOURNAL   Cell Signal 101, 110503 (2023)
   PUBMED   36374774
  REMARK    GeneRIF: SP1-induced PROX1-AS1 contributes to tumor progression by
            regulating miR-326/FBXL20 axis in colorectal cancer.
REFERENCE   2  (bases 1 to 10332)
  AUTHORS   Sun,R. and Lim,S.O.
  TITLE     FBXL20-mediated ubiquitination triggers the proteasomal degradation
            of 4-1BB
  JOURNAL   FEBS J 289 (15), 4549-4563 (2022)
   PUBMED   35112462
  REMARK    GeneRIF: FBXL20-mediated ubiquitination triggers the proteasomal
            degradation of 4-1BB.
REFERENCE   3  (bases 1 to 10332)
  AUTHORS   Madduri,L.S.V., Brandquist,N.D., Palanivel,C., Talmon,G.A.,
            Baine,M.J., Zhou,S., Enke,C.A., Johnson,K.R., Ouellette,M.M. and
            Yan,Y.
  TITLE     p53/FBXL20 axis negatively regulates the protein stability of
            PR55alpha, a regulatory subunit of PP2A Ser/Thr phosphatase
  JOURNAL   Neoplasia 23 (12), 1192-1203 (2021)
   PUBMED   34731788
REFERENCE   4  (bases 1 to 10332)
  AUTHORS   Wu,D., Liu,C. and Hong,L.
  TITLE     F-Box and Leucine-Rich Repeat Protein 20 (FBXL20), Negatively
            Regulated by microRNA (miR)-195-5p, Accelerates the Malignant
            Progression of Ovarian Cancer
  JOURNAL   Mol Biotechnol 63 (12), 1235-1243 (2021)
   PUBMED   34338995
  REMARK    GeneRIF: F-Box and Leucine-Rich Repeat Protein 20 (FBXL20),
            Negatively Regulated by microRNA (miR)-195-5p, Accelerates the
            Malignant Progression of Ovarian Cancer.
REFERENCE   5  (bases 1 to 10332)
  AUTHORS   Manne,R.K., Agrawal,Y., Malonia,S.K., Banday,S., Edachery,S.,
            Patel,A., Kumar,A., Shetty,P. and Santra,M.K.
  TITLE     FBXL20 promotes breast cancer malignancy by inhibiting apoptosis
            through degradation of PUMA and BAX
  JOURNAL   J Biol Chem 297 (4), 101253 (2021)
   PUBMED   34587475
  REMARK    GeneRIF: FBXL20 promotes breast cancer malignancy by inhibiting
            apoptosis through degradation of PUMA and BAX.
REFERENCE   6  (bases 1 to 10332)
  AUTHORS   Xiao,J., Zhang,T., Xu,D., Wang,H., Cai,Y., Jin,T., Liu,M., Jin,M.,
            Wu,K. and Yuan,J.
  TITLE     FBXL20-mediated Vps34 ubiquitination as a p53 controlled checkpoint
            in regulating autophagy and receptor degradation
  JOURNAL   Genes Dev 29 (2), 184-196 (2015)
   PUBMED   25593308
  REMARK    GeneRIF: which provides a signal to promote its ubiquitination and
            proteasomal degradation mediated by FBXL20 and the associated
            Skp1-Cullin1 complex, leading to inhibition of autophagy and
            receptor endocytosis
REFERENCE   7  (bases 1 to 10332)
  AUTHORS   Zhu,J., Li,K., Dong,L. and Chen,Y.
  TITLE     Role of FBXL20 in human colorectal adenocarcinoma
  JOURNAL   Oncol Rep 28 (6), 2290-2298 (2012)
   PUBMED   23023584
  REMARK    GeneRIF: study concludes FBXL20 plays an essential role in the
            carcinogenesis of colorectal adenocarcinoma and promotes
            carcinogenesis through the regulation of the Wnt signaling pathway
            and caspase activation
REFERENCE   8  (bases 1 to 10332)
  AUTHORS   Mells,G.F., Floyd,J.A., Morley,K.I., Cordell,H.J., Franklin,C.S.,
            Shin,S.Y., Heneghan,M.A., Neuberger,J.M., Donaldson,P.T., Day,D.B.,
            Ducker,S.J., Muriithi,A.W., Wheater,E.F., Hammond,C.J.,
            Dawwas,M.F., Jones,D.E., Peltonen,L., Alexander,G.J., Sandford,R.N.
            and Anderson,C.A.
  CONSRTM   UK PBC Consortium; Wellcome Trust Case Control Consortium 3
  TITLE     Genome-wide association study identifies 12 new susceptibility loci
            for primary biliary cirrhosis
  JOURNAL   Nat Genet 43 (4), 329-332 (2011)
   PUBMED   21399635
  REMARK    Erratum:[Nat Genet. 2011 Nov;43(11):1164. Richardson,Paul [added];
            Nasr, Ikram [added]; Aspinall,Richard [added]]
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 10332)
  AUTHORS   Ebstein,F., Lange,N., Urban,S., Seifert,U., Kruger,E. and
            Kloetzel,P.M.
  TITLE     Maturation of human dendritic cells is accompanied by functional
            remodelling of the ubiquitin-proteasome system
  JOURNAL   Int J Biochem Cell Biol 41 (5), 1205-1215 (2009)
   PUBMED   19028597
REFERENCE   10 (bases 1 to 10332)
  AUTHORS   Jin,J., Cardozo,T., Lovering,R.C., Elledge,S.J., Pagano,M. and
            Harper,J.W.
  TITLE     Systematic analysis and nomenclature of mammalian F-box proteins
  JOURNAL   Genes Dev 18 (21), 2573-2580 (2004)
   PUBMED   15520277
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA747594.1, BC007557.1 and AC015910.10.
            
            On Nov 23, 2018 this sequence version replaced NM_032875.2.
            
            Summary: Members of the F-box protein family, such as FBXL20, are
            characterized by an approximately 40-amino acid F-box motif. SCF
            complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM
            603134), and F-box proteins, act as protein-ubiquitin ligases.
            F-box proteins interact with SKP1 through the F box, and they
            interact with ubiquitination targets through other protein
            interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied
            by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.47178.1,
                                           SRR1660809.181234.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264658.11/ ENSP00000264658.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 DA747594.1         31-33
            4-2408              BC007557.1         1-2405
            2409-10332          AC015910.10        20588-28511
FEATURES             Location/Qualifiers
     source          1..10332
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q12"
     gene            1..10332
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /note="F-box and leucine rich repeat protein 20"
                     /db_xref="GeneID:84961"
                     /db_xref="HGNC:HGNC:24679"
                     /db_xref="MIM:609086"
     exon            1..266
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /inference="alignment:Splign:2.1.0"
     CDS             225..1535
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /note="isoform 1 is encoded by transcript variant 1; F-box
                     protein FBL2"
                     /codon_start=1
                     /product="F-box/LRR-repeat protein 20 isoform 1"
                     /protein_id="NP_116264.2"
                     /db_xref="CCDS:CCDS32640.1"
                     /db_xref="GeneID:84961"
                     /db_xref="HGNC:HGNC:24679"
                     /db_xref="MIM:609086"
                     /translation="MRRDVNGVTKSRFEMFSNSDEAVINKKLPKELLLRIFSFLDVVT
                     LCRCAQVSRAWNVLALDGSNWQRIDLFDFQRDIEGRVVENISKRCGGFLRKLSLRGCL
                     GVGDNALRTFAQNCRNIEVLNLNGCTKTTDATCTSLSKFCSKLRHLDLASCTSITNMS
                     LKALSEGCPLLEQLNISWCDQVTKDGIQALVRGCGGLKALFLKGCTQLEDEALKYIGA
                     HCPELVTLNLQTCLQITDEGLITICRGCHKLQSLCASGCSNITDAILNALGQNCPRLR
                     ILEVARCSQLTDVGFTTLARNCHELEKMDLEECVQITDSTLIQLSIHCPRLQVLSLSH
                     CELITDDGIRHLGNGACAHDQLEVIELDNCPLITDASLEHLKSCHSLERIELYDCQQI
                     TRAGIKRLRTHLPNIKVHAYFAPVTPPPSVGGSRQRFCRCCIIL"
     exon            267..328
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /inference="alignment:Splign:2.1.0"
     exon            329..383
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /inference="alignment:Splign:2.1.0"
     exon            384..458
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /inference="alignment:Splign:2.1.0"
     exon            459..553
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /inference="alignment:Splign:2.1.0"
     exon            554..622
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /inference="alignment:Splign:2.1.0"
     exon            623..718
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /inference="alignment:Splign:2.1.0"
     exon            719..845
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /inference="alignment:Splign:2.1.0"
     exon            846..920
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1051
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1112
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..1157
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1214
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /inference="alignment:Splign:2.1.0"
     exon            1215..1427
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..10332
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10315..10320
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /note="hexamer: AAGAAA"
     polyA_site      10332
                     /gene="FBXL20"
                     /gene_synonym="Fbl2; Fbl20"
                     /note="major polyA site"
ORIGIN      
        1 ggagagagcc gagctagcga cgagcagtcg ttgcggccgc cggcgccgcg ggaggtggtg
       61 gaggcctagc cggagccgag aggtctcttg ttcccgtccc acggtcccgg cgtcacccct
      121 ccggcgccca gtccccgtcc cggaactccc gggcctgtcc tgggcccccg gtctgtgcac
      181 tccgctcgcc gcagcgcccg gcccgggccg cacccgccgg ccccatgagg agggacgtga
      241 acggagtgac caagagcagg tttgagatgt tctcaaatag tgatgaagct gtaatcaata
      301 aaaaacttcc caaagaactc ctgttacgga tattttcttt tctagatgtt gttaccctgt
      361 gccgctgtgc tcaggtctcc agggcctgga atgttctggc tctggatggc agtaactggc
      421 agcgaattga cctatttgat ttccagaggg atattgaggg ccgagtagtg gagaatattt
      481 caaaacgatg tgggggcttt ttacgaaagt taagtcttcg tggatgtctt ggagtgggag
      541 acaatgcatt aagaaccttt gcacaaaact gcaggaacat tgaagtactg aatctaaatg
      601 ggtgtacaaa gacaacagac gctacatgta ctagccttag caagttctgt tccaaactca
      661 ggcaccttga cttggcttcc tgtacatcaa taacaaacat gtctctaaaa gctctgagtg
      721 agggatgtcc actgttggag cagttgaaca tttcctggtg tgaccaagta accaaggatg
      781 gcattcaagc actagtgagg ggctgtgggg gtctcaaggc cttattctta aaaggctgca
      841 cgcagctaga agatgaagct ctcaagtaca taggtgcaca ctgccctgaa ctggtgactt
      901 tgaacttgca gacttgcttg caaatcacag atgaaggtct cattactata tgcagagggt
      961 gccataagtt acaatccctt tgtgcctctg gctgctccaa catcacagat gccatcctga
     1021 atgctctagg tcagaactgc ccacggctta gaatattgga agtggcaaga tgttctcaat
     1081 taacagatgt gggctttacc actctagcca ggaattgcca tgaacttgaa aagatggacc
     1141 tggaagagtg tgttcagata acagatagca cattaatcca actttctata cactgtcctc
     1201 gacttcaagt attgagtctg tctcactgtg agctgatcac agatgatgga attcgtcacc
     1261 tggggaatgg ggcctgcgcc catgaccagc tggaggtgat tgagctggac aactgcccac
     1321 taatcacaga tgcatccctg gagcacttga agagctgtca tagccttgag cggatagaac
     1381 tctatgactg ccagcaaatc acacgggctg gaatcaagag actcaggacc catttaccca
     1441 atattaaagt ccacgcctac ttcgcacctg tcactccacc cccatcagta gggggcagca
     1501 gacagcgctt ctgcagatgc tgcatcatcc tatgacaatg gaggtggtca accttggcga
     1561 actgagtatt taatgacact tctagagcta ccgtggagtc tctccagtgg aagcaacccc
     1621 agtgttctga gcaagggtta caaagtgagg gagggcagtg tccagatccc cagagccaca
     1681 catacataca catacacacc cttaccccca tccactctag ctttgtgacc atgggactga
     1741 agtttgtgat ggctttttta tcaagtagat tggtaaaatt taaccattcc tgttgaggtg
     1801 cccataagaa aatcataggc caagataggg aggggcattc cagcaaaccc cgtgttaatg
     1861 ctactgtggt ttttaaattt ttgtctaggg gtttctttgg ggattttaga acagcatctg
     1921 ctgtcctccg gggtcaagaa aagcatggaa agacaatata tgatgtaccc agggaccaga
     1981 aagaaaattt ctttgcatct tagaaatggt agacattcat tgtgactaaa gagcttctat
     2041 gcttccttgt ttccatgcca acatgctgag catgctcaca aagaaggctc gtccattcct
     2101 cctgtgtttt agtatttggc ccagaggttt cctaaatggt tgccttgaaa tcactgtggt
     2161 ccaaatgtaa ttcttacaca ctcaaattat cactgtctgt agcacacttg tgcacctgtc
     2221 ttacattctc tgttgctccc ccccacactc ttgctcagtc tgtcacctgt tcagtctgct
     2281 tactcactca attgttaccc ttttgctgtt gtcgtgttta cagtttgcat tttgaatgat
     2341 tagttgggat taccaaacat tttttaaaaa gatattatca ataaatattt ttttaattct
     2401 aaattttacc attaggggac cctgcctgaa tctttgccaa cctgcaagga aactctaatg
     2461 aaagcaagaa aagttatgag aagaaactgt tttgatgaaa acagatgcta ccttttggtt
     2521 tctattttat cttctgatat gtgataattc gcatggtatc cccaatattc cccctacata
     2581 ttcttccttc tgtcagactc caccaactgt atcataagga aagagagaga tgtattatta
     2641 ggaaagagtg aaaatgataa atcacaaaac agagcctgaa agtccccaac acccaagttc
     2701 agacattaat gattaatgtt attatatatt tactgaaatc taggagtgat cagtggcatc
     2761 tctgacctat gaggttgttt taacaggaat gccactagag gagctgtgta ttctagccag
     2821 tctgtttctc tcttttcccc catttcagcc tttcagggat acaagaatgg ctctataaca
     2881 gctggtcaat tgtatgtcag tgttaatgga ttcatatcta gtgttggcac cccaaagggc
     2941 cagaaccttc ccataacctg tcagtcatct ctcttttctg ccccagtctt tttttttttt
     3001 tttttttttt tttaagacgg gttctcactc ttgcccagac tggagtgcag tggtgcgatc
     3061 tcggctcacc gcaacctcca cttcccaggc tcaagcgatt ctcttgcctc agcctaccga
     3121 gtagctggga ttacaggcat gcaccactac tgcctggcta atttttgtat ttttagtaga
     3181 gatggggttt cacatgttgg ccagcctggt cttgaactcc tgacctcaaa tgatccacct
     3241 gcctcagcct cccaaagtgc tgggactaca ggcatgagcc accatgcccg gccctgttca
     3301 gacttctgaa tgaagtatga atgtagccag gtgaagcatt gaggcagctg aaagagaaca
     3361 ctgagaggaa gtaaggggtt tagtgtctct gcttagcctg ggcactagaa ctgagggagg
     3421 aatttagtgt ccaggactca agagtccaag aagaagcacc ctctgtcttc ccaaaaaatc
     3481 agaggataaa ggaagtaaaa tgagttttta aagcataaga agaaaatttc taaaccccaa
     3541 gattagaaac ccagtgtgat ttttagtagg gtgattttgg cggggggtgt cctttgagac
     3601 attaatgtgc aatccatcat aatattaatc ttctatatga tccagactat taaaaggctt
     3661 gttttattta tttattttat tttattttat ttttttagag atggggtctc gctatcttgc
     3721 ccagcctgga ctcaaactcc tgggctcaag ccttcctccc acctcagcct cccaagtagc
     3781 tgggactaca ggtgtgtgcc actgtaccca gcttaagagg tttgttttaa tgctgcagtt
     3841 gctgattata ccagcatata taagaataaa gtctgattta tttatccatt gatttagaag
     3901 gcaattggat gaaagggtct tagaagtcta tactatccat taatatttat catattaaat
     3961 ttattttaaa tcctgtttta ttgtgccgct ttaaaaacgc agattacttg tttattcatt
     4021 cccctttccc cttattctct ttcagattac cttataagga atatagtttt taacatgaaa
     4081 ctcagtttct tgtctgtacc cctacacata aaaatacata cgtgcataca cacatacttc
     4141 cagatgtggt taataatatt cagatcccta ggttactcaa acaaaactaa aggagagaac
     4201 tatttagggg ccatctgctt taaagtttaa ggtgttttgt tgttgtttta gtgcctgtac
     4261 aaattgacca taaaacccag attgattagt gagtgtggat ttgagttgcc taagttgagg
     4321 ctattaaggt acttctttac ttaaaagata agtttaaaag actctattca tcagttcatg
     4381 atgatcttgg aaatgctgtt tctctgctca aaggatatac ccacaggcag gtccatgggg
     4441 acttgctctg gtgatctctg ctattaaggc aaattccagc ctgtttgtgc caagaacaga
     4501 gtcaagtgag agagaatgaa gactcagaga tgtgcagcac tggcttgtat gttgtcaggc
     4561 agaagccttg ttcctttctc aagaaaactt caggcctatc aagcttggaa ggaaatacat
     4621 ttttttatgc tgatttgcag cctacaccag ctgatgaata tgctaggact ttgagagaac
     4681 acaaagcatg gtaagttttg gtcaggtgga aaggagcctg acatctgaac ataatgcttc
     4741 ctctctcaac atctgggata cttttgaaaa ttcagtaagg cttaatgtag gaaaaattct
     4801 ttacaggaaa agctaattga tctgtttgtc taacctcata gccactccaa aatatgcctt
     4861 ttataatgga aaaaaacagt gaacagagag aagagatttg gggataagtt agaaggtacc
     4921 ctcaaaacta ctgccgtaaa cctacaggtg ggcaaggagg aattactgta gtgtctctgg
     4981 agaaagttct tgaacatcca aatcatctct tttctaagca gtttttcccc taatctcata
     5041 ccttacatct tccagcatca tttgctacat caatgtcttt tcatcctctt catcagcccc
     5101 attgagctga ctccagctta tttttgcact gatttccacc atttccttcc cacacccatc
     5161 ctttccctct atttaaagcc tccatctcac cctcagtaat gctgctgatt tactctcaga
     5221 tgtctcaagt ctagcttcct ttttgaatcc ttctcctact ccaggggctg tcaaaggcag
     5281 ccttaaagtt cagtgactgt taggtggctc aggccatttt tcattgggtg gtctttggtg
     5341 tttgcatact gaatttgaat tcagtttaaa agcaaactgt tggatatgag gaagaaaggg
     5401 atatggcctt agcccttttt ccaatatttg gaatatgaaa gcatattgtg ctttgtgtaa
     5461 aaaatgtttt cttctcctat tctgctcttg tctttactga aggtaaaagt attccttaag
     5521 tagcttttgc aaatttctag tctgatttcc acattcatta agttgattta gcagacatgt
     5581 aagtcagaag tttcacatat cccttgtatc aaacatttta tagggaaacc tagtaaattt
     5641 atgatgcttc attttgggct ttccagacta tttgagttat tcaagatagc cataatgttt
     5701 tagtagcgac attcttttat tacttctaat tgccttgtaa aatgcattgt tatggcccgg
     5761 cacggtgggt ggatcacaag gtcaggagtt caagaacagc ctggccaaga tggtgaaatg
     5821 ctgtctctac tgaaaataca aaaattagcc aggtgtggtg gcaggtgcct gtaatcccag
     5881 ctactcggga ggctgaggca gagaattgct tgaacccggg aggtggaggt tgcagtgagc
     5941 tgagatcgca ccactgcact ccaccctggg cgacagagtg agactatctc aaaaaaaaag
     6001 aaagaaagaa agaaaaaaat gcatcgttac tagtttgata gcccactaat gccatggggg
     6061 actaaacaaa atatgtgatg aaacctgtct tggtttgaag ttgttagaaa gtgtgagagt
     6121 gtcactggaa agaagagagt ggcttcctgc ctgcttgttt agtcagcatg tccttaggtg
     6181 actgtgttgt tgggagaaga gcactcatgg gtcatgggtg acccttggcc attttatgtt
     6241 tattgtgctg aaagtcacag gcggctcatg ccagttgcta ccagtgagag gaggggttta
     6301 aaggaaattg ctctattttg ccttattcct ccttatgtgt atgatttaag ggtaggcttt
     6361 tatcctggtt gtatctcctg gctgacagac tttctcaatc atatgtttga gctctgaagc
     6421 attttacaaa aagattgcct ctctatctgc ctgccaatcc tagaatcttt agccacctca
     6481 aacccaggca ctgtgaatta gccaagtcct gtttgagaag aaatgtagga tgtttggttg
     6541 gaattcttga gacagaacct ctctccccat taatgatgtt gtaccaaaga attctaaagc
     6601 agtaaaagga taatggggaa aaatggagtc acagcctttc ctcgcctata tttctttttt
     6661 tttttttttt ttttgacatg gagtctcgct ctgtcaccca ggttggagtg ccatggcaca
     6721 atctcagttc actgcaacct ctgcctcctg ggttcaagtg attcttctgt ttcagcctcc
     6781 caagaggcca ggattacagg tgcccaccac cacgcccagc taatgtttgt atttttcata
     6841 gagatggggt tttgccatgt tggccaggct agtctcgaac ttctgacctc aagtggtcca
     6901 cctgccttgg cctcccaaag tgctgggatt acaggtgtaa gccaccgcac ctggccagtt
     6961 tctatcataa attttaaatg taagattgga agatacagag agctgtataa aatcatacca
     7021 gtagggtata attagcccag gtagtttagg aataaaattg ggttgtatcc gagtgcccta
     7081 acttgacatt ttgattctcc agcaaagaac ataggttact aggttactaa ccttgccttc
     7141 tcacttggga taaacttttt ttttttttct tgttgcccag gctggagtgc aatagtgaga
     7201 tctcggctca ccgcaacctc cgcctcttgg gttcaagcga ttctcctccc ttagcctccc
     7261 gagtagctgg aattacagcc tgtgccacca cgcccggcaa aatttgtatt tttagtagag
     7321 acggggtttc accatgttgg tcaggctggt ttcaaactcc caacctcagg tgatctgccc
     7381 acctcagcct cccaacatgc taggattaca ggcgtgagcc actgcgccca gcccaggata
     7441 aacattctac ctgcctgaaa gtaccataag aggcaagtga gtttcttgca tgatgggctt
     7501 ctgcagcttc cccctcaagt cacatctacc tgttgtattt atttatttat ttatttttga
     7561 gacagagtct cgctctgttt cccaggctgg agtgcagtgg cgtgatctcg gctcactgca
     7621 agctccacct cccaggttca cgccattctc ctgcctcagc ctcccaagta gctgggacta
     7681 caggctcccg ccaccacgcc cggctaattt tttgtatttt tagtagagac ggggtttcac
     7741 cctgttagcc aggatggtct cgatctcctg acctcatgat ccacccgcct cggcctccca
     7801 aagtgctggg attacaggcg tgagccaccg cgcccggccc gttgtatcta ttttttttag
     7861 acgcatcaat tcagtgtctc tgaagccttt gatcgttagg aaggtcagta cataacctct
     7921 taggtgaagc tgagttgatc tttggagccc aagagagtta attttcagga gaaaaagaat
     7981 tagggcaagc tcccctgtgg gatgttaaga gaaagtccat atgcgcttct tccatctctg
     8041 cctcttgttt ttaaataagt gcttgtgaag ttcagtttat agaggttgag tctcagtgct
     8101 tcctcagcca gggtggttta ttgctgtttt acaaagaacc atgaacaagc aaaatcctat
     8161 caatagtcct ttctgtttta tgttttatag tcttattttg aagaatcggc atggtaaaat
     8221 gatagacaag tttcaaataa agcagataat tagctctaga attggggtac tttgcctcag
     8281 gtacagcatc tgtcttgaat acatagtgat ggctgtgtca cacatgggta catttacttt
     8341 cctctctgat caactgcacc cctttttact ggcaaagagg gctcctactt tctacacact
     8401 taaccagtca aacagatagg ttttggcacc accgaaagaa ccacaagtgc accccagctg
     8461 catctgggca tggttttggt actaggacta gagaagtgag cagtaaatga cagtccttac
     8521 cctaggaaga gatgggagaa gagaggtgtc cagacatatt taccttttct cttctgtcat
     8581 gccccaagaa ccaaggatca tctttgaagt gacgtctttt ttgttaaatt ggaagaagaa
     8641 aactttgcag ccaggcgtgg tggctcacgc ctgtaatccc agcactttgg gaggccgagg
     8701 cgggcggatc acaaagtgtc aggagtttga gaacagcctg gccaacatgg tgaaacccca
     8761 tctctactga aaatacaaaa tcccagctac ttgggaggct gaggcaagag aatcacttga
     8821 acctgggagg cggatgttgc agtgagccga gatcacgcca ctgcactcca gcctgggcaa
     8881 cggagtgaga ctccatctca caaaaaagag aaaagaaaag aaaactttgc ttcactaagt
     8941 ttctcttgcc attatctcat taattcagat ctgtacctca ttgctgctca cacatatatg
     9001 tttcttacct tagcctgaac cttctcaggc cttgaagtca ttattctact cttttttttt
     9061 cttttccttt tttatggcac tagagaatga aaacctaagg tcttagaaaa tcttaacttg
     9121 aaaaagtctc taactggcat gatttgatta cttttttccc ctgtggacaa ataattttct
     9181 ttcctatata atagattttt agtaagtgaa acctttgctg ctgctttcct gggtttacat
     9241 tagttttttc ctgcccttaa gaataattta tctgtgcttc tctgatgact gttaatttct
     9301 aatagtgcag gaagggatga gcttttgatt tcttctcact cccctcctca ccctacttcc
     9361 aaattacaca ctaacttcta acccttacag cctgttggca catacacaag atccacgaga
     9421 ccatgctcct cagaagctac ccatgtcctt tctcaccatg gtgtggctct tggagccctg
     9481 agtatctgtg actacccact ccaaaggttg actgtaagag gagagcggcc ttcttaattt
     9541 tgcatttctt aattttgttt ttctccattc ctgttttatg acttggcctt cagatgcttc
     9601 ctactctggc ctcctccttt tttctcctag gaattgtttc caggtaacct accatgtgca
     9661 cttctcagct gctctgctca cctttgccca ccttcccgtg accctgagag tacagatccg
     9721 aataatgtgg cctgtgcaga gctcagagaa ctgtgaggac tacccatgcc tgtcagactc
     9781 tgctcaggga cagagatgga tgggtaaatg gtgctgttgg agacattttt atcttcatac
     9841 caggctctgt ttcatccctg caccccgcac ccccactcac tcccctgagg gggtaaaatg
     9901 cagaggtggt tgacctgaag ggtctgattc cctccttccc aagccttagg gcctaccctg
     9961 gagtgctgca gtgtgtgaga gctgctgctt gcttgtttct ccactaggcc tgctccaaat
    10021 gcttagccaa tctctggagc cgacacagtt gcctacgggg tgacttagca ccaggttcca
    10081 ttagctctcc aagtgactgt ttccacccag tacttttggc gcttgaggat ggggggaggg
    10141 ggattattta aaataaataa aatccgaggt ggcagtagag gattcctttt gctttaaaaa
    10201 tctttggact aaaaaaaaaa aaaaaaagtt ttttatatat aaatatataa agtaaattgt
    10261 tatgtaacta ttattgtttc ttgtatgttc taattttgtt ttatgatgta attaaagaaa
    10321 gtaagctgtg aa
//
