LOCUS       NM_004104               8464 bp    mRNA    linear   PRI 24-OCT-2024
DEFINITION  Homo sapiens fatty acid synthase (FASN), mRNA.
ACCESSION   NM_004104
VERSION     NM_004104.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Zhong,X., Zhang,W., Zhang,W., Yu,N., Li,W. and Song,X.
  TITLE     FASN contributes to ADM resistance of diffuse large B-cell lymphoma
            by inhibiting ferroptosis via nf-kappaB/STAT3/GPX4 axis
  JOURNAL   Cancer Biol Ther 25 (1), 2403197 (2024)
   PUBMED   39345091
  REMARK    GeneRIF: FASN contributes to ADM resistance of diffuse large B-cell
            lymphoma by inhibiting ferroptosis via nf-kappaB/STAT3/GPX4 axis.
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Kang,J., Wu,J., Liu,Q., Jiang,H., Li,W., Li,Y., Li,X., Ni,C.,
            Wu,L., Liu,M., Liu,H., Deng,L., Lin,Z., Wu,X., Zhao,Y. and Ren,J.
  TITLE     FASN regulates STING palmitoylation via malonyl-CoA in macrophages
            to alleviate sepsis-induced liver injury
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (7), 167299 (2024)
   PUBMED   38878833
  REMARK    GeneRIF: FASN regulates STING palmitoylation via malonyl-CoA in
            macrophages to alleviate sepsis-induced liver injury.
REFERENCE   3  (bases 1 to 8464)
  AUTHORS   Liu,Z., Hu,Q., Cao,K., Sun,J., Cui,L., Ji,M., Shan,W., Yang,W.,
            Zhang,G., Tian,Z., Shi,H., Zhang,B. and Wang,R.
  TITLE     Deficiency of SIAH1 promotes the formation of filopodia by
            increasing the accumulation of FASN in liver cancer
  JOURNAL   Cell Death Dis 15 (7), 537 (2024)
   PUBMED   39075049
  REMARK    GeneRIF: Deficiency of SIAH1 promotes the formation of filopodia by
            increasing the accumulation of FASN in liver cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8464)
  AUTHORS   Papulino,C., Chianese,U., Ali,A., Favale,G., Tuccillo,C.,
            Ciardiello,F., Di Mauro,A., Mignogna,C., Ferrara,G., Budillon,A.,
            Megchelenbrink,W.L., Del Gaudio,N., Conte,M., Merciai,F.,
            Campiglia,P., Altucci,L., Carafa,V., Sommella,E. and Benedetti,R.
  TITLE     Inverse FASN and LDHA correlation drives metabolic resistance in
            breast cancer
  JOURNAL   J Transl Med 22 (1), 676 (2024)
   PUBMED   39044184
  REMARK    GeneRIF: Inverse FASN and LDHA correlation drives metabolic
            resistance in breast cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8464)
  AUTHORS   Cigliano,A., Simile,M.M., Vidili,G., Pes,G.M., Dore,M.P., Urigo,F.,
            Cossu,E., Che,L., Feo,C., Steinmann,S.M., Ribback,S., Pascale,R.M.,
            Evert,M., Chen,X. and Calvisi,D.F.
  TITLE     Fatty Acid Synthase Promotes Hepatocellular Carcinoma Growth via
            S-Phase Kinase-Associated Protein 2/p27KIP1 Regulation
  JOURNAL   Medicina (Kaunas) 60 (7), 1160 (2024)
   PUBMED   39064589
  REMARK    GeneRIF: Fatty Acid Synthase Promotes Hepatocellular Carcinoma
            Growth via S-Phase Kinase-Associated Protein 2/p27[KIP1]
            Regulation.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8464)
  AUTHORS   Jayakumar,A., Tai,M.H., Huang,W.Y., al-Feel,W., Hsu,M.,
            Abu-Elheiga,L., Chirala,S.S. and Wakil,S.J.
  TITLE     Human fatty acid synthase: properties and molecular cloning
  JOURNAL   Proc Natl Acad Sci U S A 92 (19), 8695-8699 (1995)
   PUBMED   7567999
REFERENCE   7  (bases 1 to 8464)
  AUTHORS   Semenkovich,C.F., Coleman,T. and Fiedorek,F.T. Jr.
  TITLE     Human fatty acid synthase mRNA: tissue distribution, genetic
            mapping, and kinetics of decay after glucose deprivation
  JOURNAL   J Lipid Res 36 (7), 1507-1521 (1995)
   PUBMED   7595075
REFERENCE   8  (bases 1 to 8464)
  AUTHORS   Wakil,S.J.
  TITLE     Fatty acid synthase, a proficient multifunctional enzyme
  JOURNAL   Biochemistry 28 (11), 4523-4530 (1989)
   PUBMED   2669958
  REMARK    Review article
REFERENCE   9  (bases 1 to 8464)
  AUTHORS   Lejin,D.
  TITLE     [Viscosimetry in clinical practice]
  JOURNAL   Med Pregl 30 (9-10), 477-482 (1977)
   PUBMED   600212
REFERENCE   10 (bases 1 to 8464)
  AUTHORS   Wronkowski,Z.
  TITLE     [Cancer diagnosis of the respiratory system]
  JOURNAL   Pieleg Polozna (12), 7-8 (1975)
   PUBMED   1044453
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC135056.4, BC063242.1,
            BC021544.2 and BC007267.1.
            
            On Nov 23, 2018 this sequence version replaced NM_004104.4.
            
            Summary: The enzyme encoded by this gene is a multifunctional
            protein. Its main function is to catalyze the synthesis of
            palmitate from acetyl-CoA and malonyl-CoA, in the presence of
            NADPH, into long-chain saturated fatty acids. In some cancer cell
            lines, this protein has been found to be fused with estrogen
            receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused
            in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC063242.1, AB209988.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000306749.4/ ENSP00000304592.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 AC135056.4         64594-64599         c
            7-3574              BC063242.1         1-3568
            3575-5464           BC021544.2         159-2048
            5465-7800           BC007267.1         601-2936
            7801-8464           BC063242.1         7795-8458
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.3"
     gene            1..8464
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /note="fatty acid synthase"
                     /db_xref="GeneID:2194"
                     /db_xref="HGNC:HGNC:3594"
                     /db_xref="MIM:600212"
     exon            1..116
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            117..250
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..7659
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /EC_number="2.3.1.85"
                     /EC_number="2.3.1.38"
                     /EC_number="2.3.1.39"
                     /EC_number="2.3.1.41"
                     /EC_number="3.1.2.14"
                     /EC_number="4.2.1.59"
                     /EC_number="1.1.1.100"
                     /EC_number="1.3.1.39"
                     /note="short chain dehydrogenase/reductase family 27X,
                     member 1; type I fatty acid synthase;
                     [Acyl-carrier-protein] S-acetyltransferase;
                     [Acyl-carrier-protein] S-malonyltransferase;
                     3-oxoacyl-[acyl-carrier-protein] synthase;
                     3-oxoacyl-[acyl-carrier-protein] reductase;
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase;
                     Enoyl-[acyl-carrier-protein] reductase;
                     Acyl-[acyl-carrier-protein] hydrolase"
                     /codon_start=1
                     /product="fatty acid synthase"
                     /protein_id="NP_004095.4"
                     /db_xref="CCDS:CCDS11801.1"
                     /db_xref="GeneID:2194"
                     /db_xref="HGNC:HGNC:3594"
                     /db_xref="MIM:600212"
                     /translation="MEEVVIAGMSGKLPESENLQEFWDNLIGGVDMVTDDDRRWKAGL
                     YGLPRRSGKLKDLSRFDASFFGVHPKQAHTMDPQLRLLLEVTYEAIVDGGINPDSLRG
                     THTGVWVGVSGSETSEALSRDPETLVGYSMVGCQRAMMANRLSFFFDFRGPSIALDTA
                     CSSSLMALQNAYQAIHSGQCPAAIVGGINVLLKPNTSVQFLRLGMLSPEGTCKAFDTA
                     GNGYCRSEGVVAVLLTKKSLARRVYATILNAGTNTDGFKEQGVTFPSGDIQEQLIRSL
                     YQSAGVAPESFEYIEAHGTGTKVGDPQELNGITRALCATRQEPLLIGSTKSNMGHPEP
                     ASGLAALAKVLLSLEHGLWAPNLHFHSPNPEIPALLDGRLQVVDQPLPVRGGNVGINS
                     FGFGGSNVHIILRPNTQPPPAPAPHATLPRLLRASGRTPEAVQKLLEQGLRHSQDLAF
                     LSMLNDIAAVPATAMPFRGYAVLGGERGGPEVQQVPAGERPLWFICSGMGTQWRGMGL
                     SLMRLDRFRDSILRSDEAVKPFGLKVSQLLLSTDESTFDDIVHSFVSLTAIQIGLIDL
                     LSCMGLRPDGIVGHSLGEVACGYADGCLSQEEAVLAAYWRGQCIKEAHLPPGAMAAVG
                     LSWEECKQRCPPGVVPACHNSKDTVTISGPQAPVFEFVEQLRKEGVFAKEVRTGGMAF
                     HSYFMEAIAPPLLQELKKVIREPKPRSARWLSTSIPEAQWHSSLARTSSAEYNVNNLV
                     SPVLFQEALWHVPEHAVVLEIAPHALLQAVLKRGLKPSCTIIPLMKKDHRDNLEFFLA
                     GIGRLHLSGIDANPNALFPPVEFPAPRGTPLISPLIKWDHSLAWDVPAAEDFPNGSGS
                     PSAAIYNIDTSSESPDHYLVDHTLDGRVLFPATGYLSIVWKTLARALGLGVEQLPVVF
                     EDVVLHQATILPKTGTVSLEVRLLEASRAFEVSENGNLVVSGKVYQWDDPDPRLFDHP
                     ESPTPNPTEPLFLAQAEVYKELRLRGYDYGPHFQGILEASLEGDSGRLLWKDNWVSFM
                     DTMLQMSILGSAKHGLYLPTRVTAIHIDPATHRQKLYTLQDKAQVADVVVSRWLRVTV
                     AGGVHISGLHTESAPRRQQEQQVPILEKFCFTPHTEEGCLSERAALQEELQLCKGLVQ
                     ALQTKVTQQGLKMVVPGLDGAQIPRDPSQQELPRLLSAACRLQLNGNLQLELAQVLAQ
                     ERPKLPEDPLLSGLLDSPALKACLDTAVENMPSLKMKVVEVLAGHGHLYSRIPGLLSP
                     HPLLQLSYTATDRHPQALEAAQAELQQHDVAQGQWDPADPAPSALGSADLLVCNCAVA
                     ALGDPASALSNMVAALREGGFLLLHTLLRGHPLGDIVAFLTSTEPQYGQGILSQDAWE
                     SLFSRVSLRLVGLKKSFYGSTLFLCRRPTPQDSPIFLPVDDTSFRWVESLKGILADED
                     SSRPVWLKAINCATSGVVGLVNCLRREPGGNRLRCVLLSNLSSTSHVPEVDPGSAELQ
                     KVLQGDLVMNVYRDGAWGAFRHFLLEEDKPEEPTAHAFVSTLTRGDLSSIRWVCSSLR
                     HAQPTCPGAQLCTVYYASLNFRDIMLATGKLSPDAIPGKWTSQDSLLGMEFSGRDASG
                     KRVMGLVPAKGLATSVLLSPDFLWDVPSNWTLEEAASVPVVYSTAYYALVVRGRVRPG
                     ETLLIHSGSGGVGQAAIAIALSLGCRVFTTVGSAEKRAYLQARFPQLDSTSFANSRDT
                     SFEQHVLWHTGGKGVDLVLNSLAEEKLQASVRCLATHGRFLEIGKFDLSQNHPLGMAI
                     FLKNVTFHGVLLDAFFNESSADWREVWALVQAGIRDGVVRPLKCTVFHGAQVEDAFRY
                     MAQGKHIGKVVVQVLAEEPEAVLKGAKPKLMSAISKTFCPAHKSYIIAGGLGGFGLEL
                     AQWLIQRGVQKLVLTSRSGIRTGYQAKQVRRWRRQGVQVQVSTSNISSLEGARGLIAE
                     AAQLGPVGGVFNLAVVLRDGLLENQTPEFFQDVCKPKYSGTLNLDRVTREACPELDYF
                     VVFSSVSCGRGNAGQSNYGFANSAMERICEKRRHEGLPGLAVQWGAIGDVGILVETMS
                     TNDTIVSGTLPQRMASCLEVLDLFLNQPHMVLSSFVLAEKAAAYRDRDSQRDLVEAVA
                     HILGIRDLAAVNLDSSLADLGLDSLMSVEVRQTLERELNLVLSVREVRQLTLRKLQEL
                     SSKADEASELACPTPKEDGLAQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVH
                     PIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAG
                     YSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAE
                     TEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARS
                     FYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGD
                     HRTLLEGSGLESIISIIHSSLAEPRVSVREG"
     exon            251..403
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            404..577
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            578..778
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            779..901
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1017
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1152
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1615
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            1616..1803
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            1804..1993
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            1994..2088
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            2089..2223
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            2224..2427
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            2428..2543
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            2544..2716
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            2717..2908
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            2909..2989
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            2990..3166
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            3167..3346
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            3347..3550
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            3551..3855
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            3856..4245
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            4246..4410
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            4411..4532
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            4533..4687
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            4688..4891
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            4892..5042
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            5043..5221
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            5222..5341
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            5342..5464
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            5465..5688
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            5689..5890
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            5891..6042
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            6043..6134
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            6135..6286
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            6287..6529
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            6530..6718
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            6719..6949
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            6950..7170
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            7171..7269
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            7270..7521
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     exon            7522..8464
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8443..8448
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /note="hexamer: ATTAAA"
     polyA_site      8464
                     /gene="FASN"
                     /gene_synonym="FAS; OA-519; SDR27X1"
                     /note="major polyA site"
ORIGIN      
        1 gagccagaga gacggcagcg gccccggcct ccctctccgc cgcgcttcag cctcccgctc
       61 cgccgcgctc cagcctcgct ctccgccgcc cgcaccgccg cccgcgccct caccagagca
      121 gccatggagg aggtggtgat tgccggcatg tccgggaagc tgccagagtc ggagaacttg
      181 caggagttct gggacaacct catcggcggt gtggacatgg tcacggacga tgaccgtcgc
      241 tggaaggcgg ggctctacgg cctgccccgg cggtccggca agctgaagga cctgtctagg
      301 tttgatgcct ccttcttcgg agtccacccc aagcaggcac acacgatgga ccctcagctg
      361 cggctgctgc tggaagtcac ctatgaagcc atcgtggacg gaggcatcaa cccagattca
      421 ctccgaggaa cacacactgg cgtctgggtg ggcgtgagcg gctctgagac ctcggaggcc
      481 ctgagccgag accccgagac actcgtgggc tacagcatgg tgggctgcca gcgagcgatg
      541 atggccaacc ggctctcctt cttcttcgac ttcagagggc ccagcatcgc actggacaca
      601 gcctgctcct ccagcctgat ggccctgcag aacgcctacc aggccatcca cagcgggcag
      661 tgccctgccg ccatcgtggg gggcatcaat gtcctgctga agcccaacac ctccgtgcag
      721 ttcttgaggc tggggatgct cagccccgag ggcacctgca aggccttcga cacagcgggg
      781 aatgggtact gccgctcgga gggtgtggtg gccgtcctgc tgaccaagaa gtccctggcc
      841 cggcgggtgt acgccaccat cctgaacgcc ggcaccaata cagatggctt caaggagcaa
      901 ggcgtgacct tcccctcagg ggatatccag gagcagctca tccgctcgtt gtaccagtcg
      961 gccggagtgg cccctgagtc atttgaatac atcgaagccc acggcacagg caccaaggtg
     1021 ggcgaccccc aggagctgaa tggcatcacc cgagccctgt gcgccacccg ccaggagccg
     1081 ctgctcatcg gctccaccaa gtccaacatg gggcacccgg agccagcctc ggggctggca
     1141 gccctggcca aggtgctgct gtccctggag cacgggctct gggcccccaa cctgcacttc
     1201 catagcccca accctgagat cccagcgctg ttggatgggc ggctgcaggt ggtggaccag
     1261 cccctgcccg tccgtggcgg caacgtgggc atcaactcct ttggcttcgg gggctccaac
     1321 gtgcacatca tcctgaggcc caacacgcag ccgccccccg cacccgcccc acatgccacc
     1381 ctgccccgtc tgctgcgggc cagcggacgc acccctgagg ccgtgcagaa gctgctggag
     1441 cagggcctcc ggcacagcca ggacctggct ttcctgagca tgctgaacga catcgcggct
     1501 gtccccgcca ccgccatgcc cttccgtggc tacgctgtgc tgggtggtga gcgcggtggc
     1561 ccagaggtgc agcaggtgcc cgctggcgag cgcccgctct ggttcatctg ctctgggatg
     1621 ggcacacagt ggcgcgggat ggggctgagc ctcatgcgcc tggaccgctt ccgagattcc
     1681 atcctacgct ccgatgaggc tgtgaagcca ttcggcctga aggtgtcaca gctgctgctg
     1741 agcacagacg agagcacctt tgatgacatc gtccattcgt ttgtgagcct gactgccatc
     1801 cagataggcc tcatagacct gctgagctgc atggggctga ggccagatgg catcgtcggc
     1861 cactccctgg gggaggtggc ctgtggctac gccgacggct gcctgtccca ggaggaggcc
     1921 gtcctcgctg cctactggag gggacagtgc atcaaagaag cccatctccc gccgggcgcc
     1981 atggcagccg tgggcttgtc ctgggaggag tgtaaacagc gctgcccccc gggcgtggtg
     2041 cccgcctgcc acaactccaa ggacacagtc accatctcgg gacctcaggc cccggtgttt
     2101 gagttcgtgg agcagctgag gaaggagggt gtgtttgcca aggaggtgcg gaccggcggt
     2161 atggccttcc actcctactt catggaggcc atcgcacccc cactgctgca ggagctcaag
     2221 aaggtgatcc gggagccgaa gccacgttca gcccgctggc tcagcacctc tatccccgag
     2281 gcccagtggc acagcagcct ggcacgcacg tcctccgccg agtacaatgt caacaacctg
     2341 gtgagccctg tgctgttcca ggaggccctg tggcacgtgc ctgagcacgc ggtggtgctg
     2401 gagatcgcgc cccacgccct gctgcaggct gtcctgaagc gtggcctgaa gccgagctgc
     2461 accatcatcc ccctgatgaa gaaggatcac agggacaacc tggagttctt cctggccggc
     2521 atcggcaggc tgcacctctc aggcatcgac gccaacccca atgccttgtt cccacctgtg
     2581 gagttcccag ctccccgagg aactcccctc atctccccac tcatcaagtg ggaccacagc
     2641 ctggcctggg acgtgccggc cgccgaggac ttccccaacg gttcaggttc cccctcagcc
     2701 gccatctaca acatcgacac cagctccgag tctcctgacc actacctggt ggaccacacc
     2761 ctcgacggtc gcgtcctctt ccccgccact ggctacctga gcatagtgtg gaagacgctg
     2821 gcccgcgccc tgggcctggg cgtcgagcag ctgcctgtgg tgtttgagga tgtggtgctg
     2881 caccaggcca ccatcctgcc caagactggg acagtgtccc tggaggtacg gctcctggag
     2941 gcctcccgtg ccttcgaggt gtcagagaac ggcaacctgg tagtgagtgg gaaggtgtac
     3001 cagtgggatg accctgaccc caggctcttc gaccacccgg aaagccccac ccccaacccc
     3061 acggagcccc tcttcctggc ccaggctgaa gtttacaagg agctgcgtct gcgtggctac
     3121 gactacggcc ctcatttcca gggcatcctg gaggccagcc tggaaggtga ctcggggagg
     3181 ctgctgtgga aggataactg ggtgagcttc atggacacca tgctgcagat gtccatcctg
     3241 ggctcggcca agcacggcct gtacctgccc acccgtgtca ccgccatcca catcgaccct
     3301 gccacccaca ggcagaagct gtacacactg caggacaagg cccaagtggc tgacgtggtg
     3361 gtgagcaggt ggctgagggt cacagtggcc ggaggcgtcc acatctccgg gctccacact
     3421 gagtcggccc cgcggcggca gcaggagcag caggtgccca tcctggagaa gttttgcttc
     3481 actccccaca cggaggaggg gtgcctgtct gagcgcgctg ccctgcagga ggagctgcaa
     3541 ctgtgcaagg ggctggtgca ggcactgcag accaaggtga cccagcaggg gctgaagatg
     3601 gtggtgcccg gactggatgg ggcccagatc ccccgggacc cctcacagca ggaactgccc
     3661 cggctgttgt cggctgcctg caggcttcag ctcaacggga acctgcagct ggagctggcg
     3721 caggtgctgg cccaggagag gcccaagctg ccagaggacc ctctgctcag cggcctcctg
     3781 gactccccgg cactcaaggc ctgcctggac actgccgtgg agaacatgcc cagcctgaag
     3841 atgaaggtgg tggaggtgct ggctggccac ggtcacctgt attcccgcat cccaggcctg
     3901 ctcagccccc atcccctgct gcagctgagc tacacggcca ccgaccgcca cccccaggcc
     3961 ctggaggctg cccaggccga gctgcagcag cacgacgttg cccagggcca gtgggatccc
     4021 gcagaccctg cccccagcgc cctgggcagc gccgacctcc tggtgtgcaa ctgtgctgtg
     4081 gctgccctcg gggacccggc ctcagctctc agcaacatgg tggctgccct gagagaaggg
     4141 ggctttctgc tcctgcacac actgctccgg gggcaccccc tcggggacat cgtggccttc
     4201 ctcacctcca ctgagccgca gtatggccag ggcatcctga gccaggacgc gtgggagagc
     4261 ctcttctcca gggtgtcgct gcgcctggtg ggcctgaaga agtccttcta cggctccacg
     4321 ctcttcctgt gccgccggcc caccccgcag gacagcccca tcttcctgcc ggtggacgat
     4381 accagcttcc gctgggtgga gtctctgaag ggcatcctgg ctgacgaaga ctcttcccgg
     4441 cctgtgtggc tgaaggccat caactgtgcc acctcgggcg tggtgggctt ggtgaactgt
     4501 ctccgccgag agcccggcgg gaaccgcctc cggtgtgtgc tgctctccaa cctcagcagc
     4561 acctcccacg tcccggaggt ggacccgggc tccgcagaac tgcagaaggt gttgcaggga
     4621 gacctggtga tgaacgtcta ccgcgacggg gcctgggggg ctttccgcca cttcctgctg
     4681 gaggaggaca agcctgagga gccgacggca catgcctttg tgagcaccct cacccggggg
     4741 gacctgtcct ccatccgctg ggtctgctcc tcgctgcgcc atgcccagcc cacctgccct
     4801 ggcgcccagc tctgcacggt ctactacgcc tccctcaact tccgcgacat catgctggcc
     4861 actggcaagc tgtcccctga tgccatccca gggaagtgga cctcccagga cagcctgcta
     4921 ggtatggagt tctcgggccg agacgccagc ggcaagcgtg tgatgggact ggtgcctgcc
     4981 aagggcctgg ccacctctgt cctgctgtca ccggacttcc tctgggatgt gccttccaac
     5041 tggacgctgg aggaggcggc ctcggtgcct gtcgtctaca gcacggccta ctacgcgctg
     5101 gtggtgcgtg ggcgggtgcg ccccggggag acgctgctca tccactcggg ctcgggcggc
     5161 gtgggccagg ccgccatcgc catcgccctc agtctgggct gccgcgtctt caccaccgtg
     5221 gggtcggctg agaagcgggc gtacctccag gccaggttcc cccagctcga cagcaccagc
     5281 ttcgccaact cccgggacac atccttcgag cagcatgtgc tgtggcacac gggcgggaag
     5341 ggcgttgacc tggtcttgaa ctccttggcg gaagagaagc tgcaggccag cgtgaggtgc
     5401 ttggctacgc acggtcgctt cctggaaatt ggcaaattcg acctttctca gaaccacccg
     5461 ctcggcatgg ctatcttcct gaagaacgtg acattccacg gggtcctact ggatgcgttc
     5521 ttcaacgaga gcagtgctga ctggcgggag gtgtgggcgc ttgtgcaggc cggcatccgg
     5581 gatggggtgg tacggcccct caagtgcacg gtgttccatg gggcccaggt ggaggacgcc
     5641 ttccgctaca tggcccaagg gaagcacatt ggcaaagtcg tcgtgcaggt gcttgcggag
     5701 gagccggagg cagtgctgaa gggggccaaa cccaagctga tgtcggccat ctccaagacc
     5761 ttctgcccgg cccacaagag ctacatcatc gctggtggtc tgggtggctt cggcctggag
     5821 ttggcgcagt ggctgataca gcgtggggtg cagaagctcg tgttgacttc tcgctccggg
     5881 atccggacag gctaccaggc caagcaggtc cgccggtgga ggcgccaggg cgtacaggtg
     5941 caggtgtcca ccagcaacat cagctcactg gagggggccc ggggcctcat tgccgaggcg
     6001 gcgcagcttg ggcccgtggg cggcgtcttc aacctggccg tggtcttgag agatggcttg
     6061 ctggagaacc agaccccaga gttcttccag gacgtctgca agcccaagta cagcggcacc
     6121 ctgaacctgg acagggtgac ccgagaggcg tgccctgagc tggactactt tgtggtcttc
     6181 tcctctgtga gctgcgggcg tggcaatgcg ggacagagca actacggctt tgccaattcc
     6241 gccatggagc gtatctgtga gaaacgccgg cacgaaggcc tcccaggcct ggccgtgcag
     6301 tggggcgcca tcggcgacgt gggcattttg gtggagacga tgagcaccaa cgacacgatc
     6361 gtcagtggca cgctgcccca gcgcatggcg tcctgcctgg aggtgctgga cctcttcctg
     6421 aaccagcccc acatggtcct gagcagcttt gtgctggctg agaaggctgc ggcctatagg
     6481 gacagggaca gccagcggga cctggtggag gccgtggcac acatcctggg catccgcgac
     6541 ttggctgctg tcaacctgga cagctcactg gcggacctgg gcctggactc gctcatgagc
     6601 gtggaggtgc gccagacgct ggagcgtgag ctcaacctgg tgctgtccgt gcgcgaggtg
     6661 cggcaactca cgctccggaa actgcaggag ctgtcctcaa aggcggatga ggccagcgag
     6721 ctggcatgcc ccacgcccaa ggaggatggt ctggcccagc agcagactca gctgaacctg
     6781 cgctccctgc tggtgaaccc ggagggcccc accctgatgc ggctcaactc cgtgcagagc
     6841 tcggagcggc ccctgttcct ggtgcaccca atcgagggct ccaccaccgt gttccacagc
     6901 ctggcctccc ggctcagcat ccccacctat ggcctgcagt gcacccgagc tgcgcccctt
     6961 gacagcatcc acagcctggc tgcctactac atcgactgca tcaggcaggt gcagcccgag
     7021 ggcccctacc gcgtggccgg ctactcctac ggggcctgcg tggcctttga aatgtgctcc
     7081 cagctgcagg cccagcagag cccagccccc acccacaaca gcctcttcct gttcgacggc
     7141 tcgcccacct acgtactggc ctacacccag agctaccggg caaagctgac cccaggctgt
     7201 gaggctgagg ctgagacgga ggccatatgc ttcttcgtgc agcagttcac ggacatggag
     7261 cacaacaggg tgctggaggc gctgctgccg ctgaagggcc tagaggagcg tgtggcagcc
     7321 gccgtggacc tgatcatcaa gagccaccag ggcctggacc gccaggagct gagctttgcg
     7381 gcccggtcct tctactacaa gctgcgtgcc gctgagcagt acacacccaa ggccaagtac
     7441 catggcaacg tgatgctact gcgcgccaag acgggtggcg cctacggcga ggacctgggc
     7501 gcggactaca acctctccca ggtatgcgac gggaaagtat ccgtccacgt catcgagggt
     7561 gaccaccgca cgctgctgga gggcagcggc ctggagtcca tcatcagcat catccacagc
     7621 tccctggctg agccacgcgt gagcgtgcgg gagggctagg cccgtgcccc cgcctgccac
     7681 cggaggtcac tccaccatcc ccaccccacc ccaccccacc cccgccatgc aacgggattg
     7741 aagggtcctg ccggtgggac cctgtccggc ccagtgccac tgccccccga ggctgctaga
     7801 tgtaggtgtt aggcatgtcc cacccacccg ccgcctccca cggcacctcg gggacaccag
     7861 agctgccgac ttggagactc ctggtctgtg aagagccggt ggtgcccgtg cccgcaggaa
     7921 ctgggctggg cctcgtgcgc ccgtggggtc tgcgcttggt ctttctgtgc ttggatttgc
     7981 atatttattg cattgctggt agagaccccc aggcctgtcc accctgccaa gactcctcag
     8041 gcagcgtgtg ggtcccgcac tctgccccca tttccccgat gtcccctgcg ggcgcgggca
     8101 gccacccaag cctgctggct gcggccccct ctcggccagg cattggctca gcccgctgag
     8161 tggggggtcg tgggccagtc cccgaggagc tgggcccctg cacaggcaca cagggcccgg
     8221 ccacacccag cggccccccg cacagccacc cgtggggtgc tgcccttatg cccggcgccg
     8281 ggcaccaact ccatgtttgg tgtttgtctg tgtttgtttt tcaagaaatg attcaaattg
     8341 ctgcttggat tttgaaattt actgtaactg tcagtgtaca cgtctggacc ccgtttcatt
     8401 tttacaccaa tttggtaaaa atgctgctct cagcctccca caattaaacc gcatgtgatc
     8461 tcca
//
