LOCUS       NM_004461               1811 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens phenylalanyl-tRNA synthetase subunit alpha (FARSA),
            mRNA.
ACCESSION   NM_004461
VERSION     NM_004461.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Feng,M., Yang,K., Wang,J., Li,G. and Zhang,H.
  TITLE     First Report of FARSA in the Regulation of Cell Cycle and Survival
            in Mantle Cell Lymphoma Cells via PI3K-AKT and FOXO1-RAG1 Axes
  JOURNAL   Int J Mol Sci 24 (2), 1608 (2023)
   PUBMED   36675119
  REMARK    GeneRIF: First Report of FARSA in the Regulation of Cell Cycle and
            Survival in Mantle Cell Lymphoma Cells via PI3K-AKT and FOXO1-RAG1
            Axes.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1811)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1811)
  AUTHORS   Krenke,K., Szczaluba,K., Bielecka,T., Rydzanicz,M., Lange,J.,
            Koppolu,A. and Ploski,R.
  TITLE     FARSA mutations mimic phenylalanyl-tRNA synthetase deficiency
            caused by FARSB defects
  JOURNAL   Clin Genet 96 (5), 468-472 (2019)
   PUBMED   31355908
  REMARK    GeneRIF: FARSA mutations mimic phenylalanyl-tRNA synthetase
            deficiency caused by FARSB defects.
REFERENCE   5  (bases 1 to 1811)
  AUTHORS   Antonellis,A., Oprescu,S.N., Griffin,L.B., Heider,A., Amalfitano,A.
            and Innis,J.W.
  TITLE     Compound heterozygosity for loss-of-function FARSB variants in a
            patient with classic features of recessive aminoacyl-tRNA
            synthetase-related disease
  JOURNAL   Hum Mutat 39 (6), 834-840 (2018)
   PUBMED   29573043
  REMARK    GeneRIF: Expression studies using fibroblasts isolated from the
            proband revealed a severe depletion of both FARSB and FARSA protein
            levels. These data indicate that the FARSB variants destabilize
            total phenylalanyl-tRNA synthetase levels, thus causing a
            loss-of-function effect.
REFERENCE   6  (bases 1 to 1811)
  AUTHORS   Grimwood,J., Gordon,L.A., Olsen,A., Terry,A., Schmutz,J.,
            Lamerdin,J., Hellsten,U., Goodstein,D., Couronne,O.,
            Tran-Gyamfi,M., Aerts,A., Altherr,M., Ashworth,L., Bajorek,E.,
            Black,S., Branscomb,E., Caenepeel,S., Carrano,A., Caoile,C.,
            Chan,Y.M., Christensen,M., Cleland,C.A., Copeland,A., Dalin,E.,
            Dehal,P., Denys,M., Detter,J.C., Escobar,J., Flowers,D.,
            Fotopulos,D., Garcia,C., Georgescu,A.M., Glavina,T., Gomez,M.,
            Gonzales,E., Groza,M., Hammon,N., Hawkins,T., Haydu,L., Ho,I.,
            Huang,W., Israni,S., Jett,J., Kadner,K., Kimball,H., Kobayashi,A.,
            Larionov,V., Leem,S.H., Lopez,F., Lou,Y., Lowry,S., Malfatti,S.,
            Martinez,D., McCready,P., Medina,C., Morgan,J., Nelson,K.,
            Nolan,M., Ovcharenko,I., Pitluck,S., Pollard,M., Popkie,A.P.,
            Predki,P., Quan,G., Ramirez,L., Rash,S., Retterer,J., Rodriguez,A.,
            Rogers,S., Salamov,A., Salazar,A., She,X., Smith,D., Slezak,T.,
            Solovyev,V., Thayer,N., Tice,H., Tsai,M., Ustaszewska,A., Vo,N.,
            Wagner,M., Wheeler,J., Wu,K., Xie,G., Yang,J., Dubchak,I.,
            Furey,T.S., DeJong,P., Dickson,M., Gordon,D., Eichler,E.E.,
            Pennacchio,L.A., Richardson,P., Stubbs,L., Rokhsar,D.S.,
            Myers,R.M., Rubin,E.M. and Lucas,S.M.
  TITLE     The DNA sequence and biology of human chromosome 19
  JOURNAL   Nature 428 (6982), 529-535 (2004)
   PUBMED   15057824
REFERENCE   7  (bases 1 to 1811)
  AUTHORS   Gevaert,K., Goethals,M., Martens,L., Van Damme,J., Staes,A.,
            Thomas,G.R. and Vandekerckhove,J.
  TITLE     Exploring proteomes and analyzing protein processing by mass
            spectrometric identification of sorted N-terminal peptides
  JOURNAL   Nat Biotechnol 21 (5), 566-569 (2003)
   PUBMED   12665801
REFERENCE   8  (bases 1 to 1811)
  AUTHORS   Moor,N., Linshiz,G. and Safro,M.
  TITLE     Cloning and expression of human phenylalanyl-tRNA synthetase in
            Escherichia coli: comparative study of purified recombinant enzymes
  JOURNAL   Protein Expr Purif 24 (2), 260-267 (2002)
   PUBMED   11858721
REFERENCE   9  (bases 1 to 1811)
  AUTHORS   Rodova,M., Ankilova,V. and Safro,M.G.
  TITLE     Human phenylalanyl-tRNA synthetase: cloning, characterization of
            the deduced amino acid sequences in terms of the structural domains
            and coordinately regulated expression of the alpha and beta
            subunits in chronic myeloid leukemia cells
  JOURNAL   Biochem Biophys Res Commun 255 (3), 765-773 (1999)
   PUBMED   10049785
REFERENCE   10 (bases 1 to 1811)
  AUTHORS   Sen,S., Zhou,H., Ripmaster,T., Hittelman,W.N., Schimmel,P. and
            White,R.A.
  TITLE     Expression of a gene encoding a tRNA synthetase-like protein is
            enhanced in tumorigenic human myeloid leukemia cells and is cell
            cycle stage- and differentiation-dependent
  JOURNAL   Proc Natl Acad Sci U S A 94 (12), 6164-6169 (1997)
   PUBMED   9177188
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK130195.1, U07424.1 and BC006495.2.
            
            On Nov 22, 2018 this sequence version replaced NM_004461.2.
            
            Summary: Aminoacyl-tRNA synthetases are a class of enzymes that
            charge tRNAs with their cognate amino acids. This gene encodes a
            product which is similar to the catalytic subunit of prokaryotic
            and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS).
            This gene product has been shown to be expressed in a
            tumor-selective and cell cycle stage- and differentiation-dependent
            manner, the first member of the tRNA synthetase gene family shown
            to exhibit this type of regulated expression [provided by RefSeq,
            Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.129187.1,
                                           SRR3476690.248516.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000314606.9/ ENSP00000320309.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AK130195.1         5-7
            4-1632              U07424.1           1-1629
            1633-1811           BC006495.2         1621-1799
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.13"
     gene            1..1811
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /note="phenylalanyl-tRNA synthetase subunit alpha"
                     /db_xref="GeneID:2193"
                     /db_xref="HGNC:HGNC:3592"
                     /db_xref="MIM:602918"
     exon            1..162
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             16..1542
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /EC_number="6.1.1.20"
                     /note="phenylalanine-tRNA synthetase alpha-subunit;
                     phenylalanine-tRNA synthetase-like, alpha subunit;
                     phenylalanyl-tRNA synthetase-like, alpha subunit;
                     phenylalanine tRNA ligase 1, alpha, cytoplasmic;
                     phenylalanyl-tRNA synthetase alpha chain; pheRS;
                     phenylalanyl-tRNA synthetase alpha subunit;
                     phenylalanine--tRNA ligase alpha chain"
                     /codon_start=1
                     /product="phenylalanine--tRNA ligase alpha subunit"
                     /protein_id="NP_004452.1"
                     /db_xref="CCDS:CCDS12287.1"
                     /db_xref="GeneID:2193"
                     /db_xref="HGNC:HGNC:3592"
                     /db_xref="MIM:602918"
                     /translation="MADGQVAELLLRRLEASDGGLDSAELAAELGMEHQAVVGAVKSL
                     QALGEVIEAELRSTKHWELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRLPSGKV
                     GFSKAMSNKWIRVDKSAADGPRVFRVVDSMEDEVQRRLQLVRGGQAEKLGEKERSELR
                     KRKLLAEVTLKTYWVSKGSAFSTSISKQETELSPEMISSGSWRDRPFKPYNFLAHGVL
                     PDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDT
                     FFLRDPAEALQLPMDYVQRVKRTHSQGGYGSQGYKYNWKLDEARKNLLRTHTTSASAR
                     ALYRLAQKKPFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADHGLTLGHLMGVLR
                     EFFTKLGITQLRFKPAYNPYTEPSMEVFSYHQGLKKWVEVGNSGVFRPEMLLPMGLPE
                     NVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPLCRLDAEPRPPPTQEAA
                     "
     exon            163..300
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /inference="alignment:Splign:2.1.0"
     exon            301..399
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /inference="alignment:Splign:2.1.0"
     exon            400..518
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /inference="alignment:Splign:2.1.0"
     exon            519..611
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /inference="alignment:Splign:2.1.0"
     exon            612..740
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /inference="alignment:Splign:2.1.0"
     exon            741..856
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /inference="alignment:Splign:2.1.0"
     exon            857..941
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1041
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1210
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1288
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1403
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1811
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1789..1794
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /note="hexamer: AATAAA"
     polyA_site      1811
                     /gene="FARSA"
                     /gene_synonym="CML33; FARSL; FARSLA; FRSA; PheHA; RILDBC2"
                     /note="major polyA site"
ORIGIN      
        1 acactggaag gagtcatggc ggatggtcag gtggcggaac tgctgctccg gcggctggag
       61 gcgtctgatg gcggcctgga cagcgccgag ttggcggctg agctgggcat ggagcaccag
      121 gcggtggtgg gcgccgtgaa gagccttcag gcgctgggcg aggtcatcga ggctgaactt
      181 cggtccacca agcactggga gcttactgcg gagggcgagg agattgcccg ggagggcagc
      241 catgaggccc gtgtgtttcg aagcattccc ccagagggcc tggcccagag cgagcttatg
      301 cgactgccca gtggcaaagt gggcttcagc aaggccatgt ccaacaagtg gattcgggtg
      361 gacaagagtg cggctgacgg gccccgggtg ttccgagtgg tggacagcat ggaggatgag
      421 gtgcagcggc ggctccagct ggtccggggg ggacaggctg agaagctggg ggagaaggag
      481 aggagcgagc tgaggaagag gaagctgttg gctgaagtga ctctgaagac ctactgggtg
      541 agcaaaggca gtgcctttag taccagcatc tccaagcaag agacagagct gagcccagag
      601 atgatctcca gtggctcttg gcgggaccgg cccttcaagc cctacaactt cttggcccac
      661 ggtgtcctcc ccgacagcgg ccaccttcac ccgctgctca aggtccgctc ccagttccga
      721 cagatcttcc tggagatggg gttcaccgag atgccgactg ataacttcat tgagagctcc
      781 ttctggaact ttgacgccct cttccagccc cagcagcacc cagcccgtga ccagcacgac
      841 accttcttcc ttcgagatcc agcggaggcc ctgcagctcc caatggacta tgtccagcgg
      901 gtcaagcgga cccactctca gggcggctac ggctcacagg ggtacaagta taactggaag
      961 ctggacgagg cccggaaaaa cctactgcga acccacacca catcagccag cgcccgtgcg
     1021 ctctaccgcc ttgcccagaa gaagcccttc actccggtca agtacttctc catcgaccgc
     1081 gtattccgga atgagaccct ggacgccacg cacctggctg agttccacca gatcgagggc
     1141 gtggtggcgg atcatggtct caccttgggc cacctcatgg gcgttctgcg ggagttcttc
     1201 accaagctgg gtatcacgca actccgcttc aagccagcct acaacccata cacagagccc
     1261 agcatggagg tgttcagcta ccaccaaggc ctgaagaagt gggtggaggt cggaaactcg
     1321 ggggtcttcc gtccagagat gctgctgccc atggggcttc ccgagaacgt gtcggtcatt
     1381 gcctggggcc tctccctgga gcgcccaacg atgatcaaat atggcatcaa caatatccgg
     1441 gagctggtgg gccacaaggt gaacctgcag atggtgtatg acagtcccct gtgccgcctg
     1501 gatgccgagc cgaggccccc tcccacacag gaggctgcgt gacatgggcc actctaggac
     1561 aggtcatcct ccccgagtcc ctgctgctgc gctcctttgc atccctggcc agtgaccttg
     1621 tatttatgag gcctctgtga ggccagcccc caccttcctc tttcccacct gtcccaggac
     1681 cagaatccca gggacagagg actgggtagc aggttccttc tgttgtcctg tgtggtgtgt
     1741 ctactgtgag ggtgggccct gaggagacct gtgggccacc tattgtctaa taaagtgggc
     1801 agttgccccc a
//
