LOCUS       NM_032228               5266 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens fatty acyl-CoA reductase 1 (FAR1), mRNA.
ACCESSION   NM_032228
VERSION     NM_032228.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ferdinandusse,S., McWalter,K., Te Brinke,H., IJlst,L.,
            Mooijer,P.M., Ruiter,J.P.N., van Lint,A.E.M., Pras-Raves,M.,
            Wever,E., Millan,F., Guillen Sacoto,M.J., Begtrup,A.,
            Tarnopolsky,M., Brady,L., Ladda,R.L., Sell,S.L., Nowak,C.B.,
            Douglas,J., Tian,C., Ulm,E., Perlman,S., Drack,A.V., Chong,K.,
            Martin,N., Brault,J., Brokamp,E., Toro,C., Gahl,W.A.,
            Macnamara,E.F., Wolfe,L., Waisfisz,Q., Zwijnenburg,P.J.G.,
            Ziegler,A., Barth,M., Smith,R., Ellingwood,S., Gaebler-Spira,D.,
            Bakhtiari,S., Kruer,M.C., van Kampen,A.H.C., Wanders,R.J.A.,
            Waterham,H.R., Cassiman,D. and Vaz,F.M.
  CONSRTM   Undiagnosed Diseases Network
  TITLE     An autosomal dominant neurological disorder caused by de novo
            variants in FAR1 resulting in uncontrolled synthesis of ether
            lipids
  JOURNAL   Genet Med 23 (4), 740-750 (2021)
   PUBMED   33239752
  REMARK    GeneRIF: An autosomal dominant neurological disorder caused by de
            novo variants in FAR1 resulting in uncontrolled synthesis of ether
            lipids.
            Erratum:[Genet Med. 2021 Dec;23(12):2467. doi:
            10.1038/s41436-021-01189-8.. PMID: 34667295]
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 5266)
  AUTHORS   Zouboulis,C.C., Nogueira da Costa,A., Fimmel,S. and Zouboulis,K.C.
  TITLE     Apocrine glands are bystanders in hidradenitis suppurativa and
            their involvement is gender specific
  JOURNAL   J Eur Acad Dermatol Venereol 34 (7), 1555-1563 (2020)
   PUBMED   32031713
REFERENCE   4  (bases 1 to 5266)
  AUTHORS   Buchert,R., Tawamie,H., Smith,C., Uebe,S., Innes,A.M., Al
            Hallak,B., Ekici,A.B., Sticht,H., Schwarze,B., Lamont,R.E.,
            Parboosingh,J.S., Bernier,F.P. and Abou Jamra,R.
  TITLE     A peroxisomal disorder of severe intellectual disability, epilepsy,
            and cataracts due to fatty acyl-CoA reductase 1 deficiency
  JOURNAL   Am J Hum Genet 95 (5), 602-610 (2014)
   PUBMED   25439727
  REMARK    GeneRIF: A peroxisomal disorder of severe intellectual disability,
            epilepsy, and cataracts due to fatty acyl-CoA reductase 1
            deficiency.
REFERENCE   5  (bases 1 to 5266)
  AUTHORS   Honsho,M., Asaoku,S., Fukumoto,K. and Fujiki,Y.
  TITLE     Topogenesis and homeostasis of fatty acyl-CoA reductase 1
  JOURNAL   J Biol Chem 288 (48), 34588-34598 (2013)
   PUBMED   24108123
  REMARK    GeneRIF: Expression of Far1 increased plasmalogen synthesis in
            wild-type Chinese hamster ovary cells, strongly suggesting that
            Far1 is a rate-limiting enzyme for plasmalogen synthesis.
REFERENCE   6  (bases 1 to 5266)
  AUTHORS   Bharti,P., Schliebs,W., Schievelbusch,T., Neuhaus,A., David,C.,
            Kock,K., Herrmann,C., Meyer,H.E., Wiese,S., Warscheid,B., Theiss,C.
            and Erdmann,R.
  TITLE     PEX14 is required for microtubule-based peroxisome motility in
            human cells
  JOURNAL   J Cell Sci 124 (Pt 10), 1759-1768 (2011)
   PUBMED   21525035
REFERENCE   7  (bases 1 to 5266)
  AUTHORS   Wanders,R.J. and Waterham,H.R.
  TITLE     Biochemistry of mammalian peroxisomes revisited
  JOURNAL   Annu Rev Biochem 75, 295-332 (2006)
   PUBMED   16756494
  REMARK    Review article
REFERENCE   8  (bases 1 to 5266)
  AUTHORS   Cheng,J.B. and Russell,D.W.
  TITLE     Mammalian wax biosynthesis. II. Expression cloning of wax synthase
            cDNAs encoding a member of the acyltransferase enzyme family
  JOURNAL   J Biol Chem 279 (36), 37798-37807 (2004)
   PUBMED   15220349
  REMARK    GeneRIF: wax monoester synthesis in mammals involves a two step
            biosynthetic pathway catalyzed by fatty acyl-CoA reductase and wax
            synthase enzymes
REFERENCE   9  (bases 1 to 5266)
  AUTHORS   Cheng,J.B. and Russell,D.W.
  TITLE     Mammalian wax biosynthesis. I. Identification of two fatty
            acyl-Coenzyme A reductases with different substrate specificities
            and tissue distributions
  JOURNAL   J Biol Chem 279 (36), 37789-37797 (2004)
   PUBMED   15220348
  REMARK    GeneRIF: fatty alcohol synthesis in mammals is accomplished by two
            fatty acyl-CoA reductase isozymes that are expressed at high levels
            in tissues known to synthesize wax monoesters and ether lipids
REFERENCE   10 (bases 1 to 5266)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA515509.1, BC017377.2 and
            BX648892.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_032228.5.
            
            Summary: The protein encoded by this gene is required for the
            reduction of fatty acids to fatty alcohols, a process that is
            required for the synthesis of monoesters and ether lipids. NADPH is
            required as a cofactor in this reaction, and 16-18 carbon saturated
            and unsaturated fatty acids are the preferred substrate. This is a
            peroxisomal membrane protein, and studies suggest that the
            N-terminus contains a large catalytic domain located on the outside
            of the peroxisome, while the C-terminus is exposed to the matrix of
            the peroxisome. Studies indicate that the regulation of this
            protein is dependent on plasmalogen levels. Mutations in this gene
            have been associated with individuals affected by severe
            intellectual disability, early-onset epilepsy, microcephaly,
            congenital cataracts, growth retardation, and spasticity (PMID:
            25439727). A pseudogene of this gene is located on chromosome 13.
            [provided by RefSeq, Jan 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY600449.1, SRR1803614.53982.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000354817.8/ ENSP00000346874.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-49                DA515509.1         10-58
            50-1806             BC017377.2         1-1757
            1807-4267           BX648892.1         2359-4819
            4268-5266           BX648892.1         4821-5819
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.3"
     gene            1..5266
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /note="fatty acyl-CoA reductase 1"
                     /db_xref="GeneID:84188"
                     /db_xref="HGNC:HGNC:26222"
                     /db_xref="MIM:616107"
     exon            1..139
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /inference="alignment:Splign:2.1.0"
     exon            140..335
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /inference="alignment:Splign:2.1.0"
     CDS             147..1694
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /EC_number="1.2.1.84"
                     /note="short chain dehydrogenase/reductase family 10E,
                     member 1; male sterility domain-containing protein 2"
                     /codon_start=1
                     /product="fatty acyl-CoA reductase 1"
                     /protein_id="NP_115604.1"
                     /db_xref="CCDS:CCDS7813.1"
                     /db_xref="GeneID:84188"
                     /db_xref="HGNC:HGNC:26222"
                     /db_xref="MIM:616107"
                     /translation="MVSIPEYYEGKNVLLTGATGFLGKVLLEKLLRSCPKVNSVYVLV
                     RQKAGQTPQERVEEVLSGKLFDRLRDENPDFREKIIAINSELTQPKLALSEEDKEVII
                     DSTNIIFHCAATVRFNENLRDAVQLNVIATRQLILLAQQMKNLEVFMHVSTAYAYCNR
                     KHIDEVVYPPPVDPKKLIDSLEWMDDGLVNDITPKLIGDRPNTYIYTKALAEYVVQQE
                     GAKLNVAIVRPSIVGASWKEPFPGWIDNFNGPSGLFIAAGKGILRTIRASNNALADLV
                     PVDVVVNMSLAAAWYSGVNRPRNIMVYNCTTGSTNPFHWGEVEYHVISTFKRNPLEQA
                     FRRPNVNLTSNHLLYHYWIAVSHKAPAFLYDIYLRMTGRSPRMMKTITRLHKAMVFLE
                     YFTSNSWVWNTENVNMLMNQLNPEDKKTFNIDVRQLHWAEYIENYCLGTKKYVLNEEM
                     SGLPAARKHLNKLRNIRYGFNTILVILIWRIFIARSQMARNIWYFVVSLCYKFLSYFR
                     ASSTMRY"
     exon            336..511
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /inference="alignment:Splign:2.1.0"
     exon            512..691
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /inference="alignment:Splign:2.1.0"
     exon            692..869
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /inference="alignment:Splign:2.1.0"
     exon            870..914
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1033
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1101
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1273
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1403
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1531
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..5266
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4392..4397
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /note="hexamer: AATAAA"
     polyA_site      4415
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /note="major polyA site"
     regulatory      5244..5249
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /note="hexamer: AAGAAA"
     polyA_site      5266
                     /gene="FAR1"
                     /gene_synonym="CSPSD; MLSTD2; PFCRD; SDR10E1"
                     /note="major polyA site"
ORIGIN      
        1 gccactgccc gtccgctctt cagcagccgg tcgcgggcgg tggaaaagcg agtgaagaga
       61 gcgcgacggc ggcggcggcg gcggcgcagc tattgctgga cggccagtgg gagagcgagg
      121 cctgagcctc tgcgtctagg atcaaaatgg tttcaatccc agaatactat gaaggcaaga
      181 acgtcctcct cacaggagct accggttttc tagggaaggt gcttctggaa aagttgctga
      241 ggtcttgtcc taaggtgaat tcagtatatg ttttggtgag gcagaaagct ggacagacac
      301 cacaagagcg agtggaagaa gtccttagtg gcaagctttt tgacagattg agagatgaaa
      361 atccagattt tagagagaaa attatagcaa tcaacagcga actcacccaa cctaaactgg
      421 ctctcagtga agaagataaa gaggtgatca tagattctac caatattata ttccactgtg
      481 cagctacagt aaggtttaat gaaaatttaa gagatgctgt tcagttaaat gtgattgcaa
      541 cgcgacagct tattctcctt gcacaacaaa tgaagaatct ggaagtgttc atgcatgtat
      601 caacagcata tgcctactgt aatcgcaagc atattgatga agtagtctat ccaccacctg
      661 tggatcccaa gaagctgatt gattctttag agtggatgga tgatggccta gtaaatgata
      721 tcacgccaaa attgatagga gacagaccta atacatacat atacacaaaa gcattggcag
      781 aatatgttgt acaacaagaa ggagcaaaac taaatgtggc aattgtaagg ccatcgattg
      841 ttggtgccag ttggaaagaa ccttttccag gatggattga taactttaat ggaccaagtg
      901 gtctctttat tgcggcaggg aaaggaattc ttcgaacaat acgtgcctcc aacaatgccc
      961 ttgcagatct tgttcctgta gatgtagttg tcaacatgag tcttgcggca gcctggtatt
     1021 ccggagttaa tagaccaaga aacatcatgg tgtataattg tacaacaggc agcactaatc
     1081 ctttccactg gggtgaagtt gagtaccatg taatttccac tttcaagagg aatcctctcg
     1141 aacaggcctt cagacggccc aatgtaaatc taacctccaa tcatctttta tatcattact
     1201 ggattgctgt aagccataag gccccagcat tcctgtatga tatctacctc aggatgactg
     1261 gaagaagccc aaggatgatg aaaacaataa ctcgtcttca caaagctatg gtgtttcttg
     1321 aatatttcac aagtaattct tgggtttgga atactgagaa tgtcaatatg ttaatgaatc
     1381 aactaaaccc tgaagataaa aagaccttca atattgatgt acggcagtta cattgggcag
     1441 aatatataga gaactactgc ttgggaacta agaagtacgt attgaatgaa gaaatgtctg
     1501 gcctccctgc agccagaaaa catctgaaca agttgcggaa tatacgttat ggttttaata
     1561 ctatccttgt gatcctcatc tggcgcattt ttattgcaag atcacaaatg gcaagaaata
     1621 tctggtactt tgtggttagt ctgtgttaca agtttttgtc atacttccga gcatccagca
     1681 ctatgagata ctgaagacca aggattcagc attagaacat ctatacatat ggtgatctaa
     1741 atgtacaaaa tgtaaaatgt ataagtcatc tcactttttg tcaagacatt aaaccatctt
     1801 agatcggagt gtgaagtaaa ttatggtata ttttatgtaa cattttaatg tttatgctca
     1861 taaaacttag tgaacacact gtgttatgcc agctcaaatc tacagtagcc accaaaacca
     1921 tgacttaata ttttgagccc tagaagaaag gggtgtgctg aggacaagag tggggaaata
     1981 ggaacactga ccagtataac tgtgcaattc tggaacatat taattaaaat aatatgcctt
     2041 aacatatagt gaatttctaa ttctaatgtt cagtgcaatg gaagacattt atttggacag
     2101 taatactagc aaagttggta gatatttgat tcttcatttt ttgttttttt cattagttga
     2161 agtgggtttt agttttgttt aaaattataa ccagcgtatt ttcacatcat tctgtaagtt
     2221 aaatgatatc aaacatgaaa gagatgttct catttttctt tttctgatta aacgtctgat
     2281 gcatatcatt tttctataag taatcagttg cttttaaaat cagaaggcta tattattcta
     2341 atgaccctat tcgatctaaa tgggtttgag aatccatatc agcaacatac gtgttttttg
     2401 acagaaagtg aaaacaaatt ccgtaaaact gttagtatca aaaagaatag gaatacagtt
     2461 ttcttttcca cattatgatc aaataaaaat cttgtgagat tgtttatgtt ttgaagcagg
     2521 aggatttctt gagatttgat ggaagtggga gttgggaagg attcaccata cttgtgttct
     2581 cctcccttta taaattttat tcataggact tttaaaaggc ttgtgctatc tttccagtga
     2641 aaactgaaag tgcattatta tggaagaatg tatttgcagg tagtaatagc attaaaaaat
     2701 atactcgtgt gtaaacacat actaattttg accttgaaag atgaattcct tcaagaaaaa
     2761 taaaataatg cttaagataa tgtgtacgaa attaaataaa ggactttatt tacatactac
     2821 ataaggtagc aaactgttga atgagtttga aggtatcact tattttttat gcatgtgatt
     2881 ttagctttag aaagaaatgc gttatagaaa caagtaatag caagaaaatt gatgtatatt
     2941 ttaatgatac attaaaattc ccttttaatc ataatagtta actctactta ctgttttaac
     3001 atacatttga tttaacaaat tgttcagcat aacacttcta attaagttta tcaagttgta
     3061 ctgtattaga taatcagcag tgtatctgga gtatgtttaa agagaacagt tcgcaataca
     3121 aaaagttaca tggagcttta catcttaact ttctttgtca atttaaatgc aatgtataaa
     3181 aagtttattt tgctattgtg aaaaactaaa tgtaaaggaa atcacctact ttcatgcagg
     3241 tgtataatct tgaaaaggaa aaatgcttcc atgttgaagc cagattttct gtagtaaaac
     3301 ttttaaatat tattttaaaa gaaatatgta tataaatatc tctatattct ttggaatgat
     3361 actaaagtct ctggtctagg accatacctt atataaaggt ataagagacc atgacaatgt
     3421 ctgaaaatgg aatagataat gatgcctttt atttaaagtg gcccacataa tatacattga
     3481 gtactccatc tctccaaatg tatttccata atgtgttgaa aacatgctaa catttgtatg
     3541 atttttatac ttctgccgaa tagacttaga atcagatgaa ttgtctgtgt gtcttgcaaa
     3601 agagttgggg acaacttggg caggcctatg aagtgcatag ggagtgtatg tcttctgaat
     3661 ggttttattg ttcttgtaat ctagcttaaa gaaatgttaa ctgggagggt gctgaggcca
     3721 ctcactgcat taattttgtg tgtttagagt tctgttgtca aaagaaaact aatgaataaa
     3781 ttagtttgtc attctagaat ttaaagttct aagattagta taaagagtat atagattgtt
     3841 aatccccacc agctagactt tgaacttaag tcagacttaa agatttgaga aattatttgt
     3901 gtcatttact agacgtgatt tttagttctg tttgattata tttcctacac aaacttctta
     3961 tttaacagga tagcctacta aattaaatgt ttcttatttc acttaactca tttgattaaa
     4021 ctgtattcta aaacatttgg ggtttttccc cctattcagt tttaatcttg gaatatgcat
     4081 ttgtaaattg tgatgtcatt gagactatat ttatatttga cttggcaaca ttaacatgtc
     4141 ctaagactta gtgcagagaa gcttggcagt acgttctttg acttaaggat ggcataaaat
     4201 aatcattttt gaacctgtgt aataaagctt gaaagcaggg aaaagaattt ccttttcccc
     4261 cttttttgtg ttgtctatag gaattaactt gggattgttt tgtgggtttt tgtttgtttt
     4321 aaatgtaaat tgagaatctt ttataagaaa taaaagcatt attgggtgcc tttgtttgta
     4381 aaccaaaaag taataaatga atccctatat ttccattata gtatttattg tatttttatg
     4441 ttctgaaaat tacccatgga acaatatgct taggattaca ggaagcagtc cttacttaca
     4501 cttcttgtct gttttaggtg tacttgttaa ttcttatgtc ctaattttat ttaattctga
     4561 gttccttaca cagcatttta gggaaagaat acaggcagga tgacactttg tgttaaagtg
     4621 ttatttttat gtattacctg gaatgaggca ggtttttttc tgttttctaa aaagagtaac
     4681 caagatacct ccagggtgtc attgggttcc agctgctctc ctccacattg aatgatatct
     4741 tgttaattta taggcacatt tgtggtaatt tatatgtcta tagagtaagt ataagagata
     4801 attcattagt aataggaatt aactgacccc ttttggatgg gggagagcat caggctgggg
     4861 tcaggtaagt gtaaatggcc ttctgagcat gctcttctag gctgactccc agccctgact
     4921 tgaaaccatt agcgctaact tgctctgttt tgagaaaaac tttccaaact tttgcatgag
     4981 aaactagaaa aaggaatgta tgccacgtaa ctggattaca gaaatgagtt aattgtctct
     5041 gtgataaaaa aaaaaaatga aatattttct tattgaatta atatttttgt cttgaagcat
     5101 tttctagtga tagaatgtat ttgtcttttt tcctggtggt accctcttag catatatctt
     5161 tgctatcctt aagatcctaa acaaatcatc tttgtcagtt aagtatagtt gcgcaaaaat
     5221 tgttaaatcc tttgtcttta ttaaagaaaa atttgagtaa cattaa
//
