LOCUS       NM_004460               2696 bp    mRNA    linear   PRI 03-SEP-2024
DEFINITION  Homo sapiens fibroblast activation protein alpha (FAP), transcript
            variant 1, mRNA.
ACCESSION   NM_004460
VERSION     NM_004460.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Oguri,N., Gi,T., Nakamura,E., Furukoji,E., Goto,H., Maekawa,K.,
            Tsuji,A.B., Nishii,R., Aman,M., Moriguchi-Goto,S., Sakae,T.,
            Azuma,M. and Yamashita,A.
  TITLE     Expression of fibroblast activation protein-alpha in human deep
            vein thrombosis
  JOURNAL   Thromb Res 241, 109075 (2024)
   PUBMED   38955058
  REMARK    GeneRIF: Expression of fibroblast activation protein-alpha in human
            deep vein thrombosis.
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Xu,S., Jiemy,W.F., Boots,A.M.H., Arends,S., van Sleen,Y.,
            Nienhuis,P.H., van der Geest,K.S.M., Heeringa,P., Brouwer,E. and
            Sandovici,M.
  TITLE     Altered Plasma Levels and Tissue Expression of Fibroblast
            Activation Protein Alpha in Giant Cell Arteritis
  JOURNAL   Arthritis Care Res (Hoboken) 76 (9), 1322-1332 (2024)
   PUBMED   38685696
  REMARK    GeneRIF: Altered Plasma Levels and Tissue Expression of Fibroblast
            Activation Protein Alpha in Giant Cell Arteritis.
REFERENCE   3  (bases 1 to 2696)
  AUTHORS   Li,R., Zhang,R., Shi,X., Jiao,X., Li,Y., Zhao,Y., Liu,T. and
            Zhang,C.
  TITLE     Expression of FAP in Oral Leukoplakia and Oral Squamous Cell
            Carcinoma
  JOURNAL   Int Dent J 74 (3), 581-588 (2024)
   PUBMED   38278714
  REMARK    GeneRIF: Expression of FAP in Oral Leukoplakia and Oral Squamous
            Cell Carcinoma.
REFERENCE   4  (bases 1 to 2696)
  AUTHORS   Engquist,E.N., Greco,A., Joosten,L.A.B., van Engelen,B.G.M.,
            Zammit,P.S. and Banerji,C.R.S.
  TITLE     FSHD muscle shows perturbation in fibroadipogenic progenitor cells,
            mitochondrial function and alternative splicing independently of
            inflammation
  JOURNAL   Hum Mol Genet 33 (2), 182-197 (2024)
   PUBMED   37856562
  REMARK    GeneRIF: FSHD muscle shows perturbation in fibroadipogenic
            progenitor cells, mitochondrial function and alternative splicing
            independently of inflammation.
REFERENCE   5  (bases 1 to 2696)
  AUTHORS   Wu,Y., Wu,C., Shi,T., Cai,Q., Wang,T., Xiong,Y., Zhang,Y.,
            Jiang,W., Lu,M., Chen,Z., Chen,J., Wang,J. and He,R.
  TITLE     FAP expression in adipose tissue macrophages promotes obesity and
            metabolic inflammation
  JOURNAL   Proc Natl Acad Sci U S A 120 (51), e2303075120 (2023)
   PUBMED   38100414
  REMARK    GeneRIF: FAP expression in adipose tissue macrophages promotes
            obesity and metabolic inflammation.
REFERENCE   6  (bases 1 to 2696)
  AUTHORS   Niedermeyer,J., Enenkel,B., Park,J.E., Lenter,M., Rettig,W.J.,
            Damm,K. and Schnapp,A.
  TITLE     Mouse fibroblast-activation protein--conserved Fap gene
            organization and biochemical function as a serine protease
  JOURNAL   Eur J Biochem 254 (3), 650-654 (1998)
   PUBMED   9688278
REFERENCE   7  (bases 1 to 2696)
  AUTHORS   Goldstein,L.A., Ghersi,G., Pineiro-Sanchez,M.L., Salamone,M.,
            Yeh,Y., Flessate,D. and Chen,W.T.
  TITLE     Molecular cloning of seprase: a serine integral membrane protease
            from human melanoma
  JOURNAL   Biochim Biophys Acta 1361 (1), 11-19 (1997)
   PUBMED   9247085
REFERENCE   8  (bases 1 to 2696)
  AUTHORS   Pineiro-Sanchez,M.L., Goldstein,L.A., Dodt,J., Howard,L., Yeh,Y.,
            Tran,H., Argraves,W.S. and Chen,W.T.
  TITLE     Identification of the 170-kDa melanoma membrane-bound gelatinase
            (seprase) as a serine integral membrane protease
  JOURNAL   J Biol Chem 272 (12), 7595-7601 (1997)
   PUBMED   9065413
  REMARK    Erratum:[J Biol Chem. 1998 May 22:273(21):13366]
REFERENCE   9  (bases 1 to 2696)
  AUTHORS   Rettig,W.J., Su,S.L., Fortunato,S.R., Scanlan,M.J., Raj,B.K.,
            Garin-Chesa,P., Healey,J.H. and Old,L.J.
  TITLE     Fibroblast activation protein: purification, epitope mapping and
            induction by growth factors
  JOURNAL   Int J Cancer 58 (3), 385-392 (1994)
   PUBMED   7519584
REFERENCE   10 (bases 1 to 2696)
  AUTHORS   Scanlan,M.J., Raj,B.K., Calvo,B., Garin-Chesa,P.,
            Sanz-Moncasi,M.P., Healey,J.H., Old,L.J. and Rettig,W.J.
  TITLE     Molecular cloning of fibroblast activation protein alpha, a member
            of the serine protease family selectively expressed in stromal
            fibroblasts of epithelial cancers
  JOURNAL   Proc Natl Acad Sci U S A 91 (12), 5657-5661 (1994)
   PUBMED   7911242
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U09278.2, BC026250.1 and
            AL832166.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_004460.4.
            
            Summary: The protein encoded by this gene is a homodimeric integral
            membrane gelatinase belonging to the serine protease family. It is
            selectively expressed in reactive stromal fibroblasts of epithelial
            cancers, granulation tissue of healing wounds, and malignant cells
            of bone and soft tissue sarcomas. This protein is thought to be
            involved in the control of fibroblast growth or
            epithelial-mesenchymal interactions during development, tissue
            repair, and epithelial carcinogenesis. Alternatively spliced
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Apr 2014].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U09278.2, BC026250.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000188790.9/ ENSP00000188790.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-736               U09278.2           91-826
            737-1179            BC026250.1         660-1102
            1180-2688           U09278.2           1270-2778
            2689-2696           AL832166.1         1530-1537
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q24.2"
     gene            1..2696
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /note="fibroblast activation protein alpha"
                     /db_xref="GeneID:2191"
                     /db_xref="HGNC:HGNC:3590"
                     /db_xref="MIM:600403"
     exon            1..124
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     CDS             119..2401
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /EC_number="3.4.14.5"
                     /EC_number="3.4.21.26"
                     /note="isoform 1 is encoded by transcript variant 1;
                     integral membrane serine protease; seprase; 170 kDa
                     melanoma membrane-bound gelatinase; dipeptidyl peptidase
                     FAP; surface-expressed protease; post-proline cleaving
                     enzyme; gelatine degradation protease FAP; prolyl
                     endopeptidase FAP"
                     /codon_start=1
                     /product="prolyl endopeptidase FAP isoform 1"
                     /protein_id="NP_004451.2"
                     /db_xref="CCDS:CCDS33311.1"
                     /db_xref="GeneID:2191"
                     /db_xref="HGNC:HGNC:3590"
                     /db_xref="MIM:600403"
                     /translation="MKTWVKIVFGVATSAVLALLVMCIVLRPSRVHNSEENTMRALTL
                     KDILNGTFSYKTFFPNWISGQEYLHQSADNNIVLYNIETGQSYTILSNRTMKSVNASN
                     YGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLSNGEFVRGNELPRPIQYLCWSPVGS
                     KLAYVYQNNIYLKQRPGDPPFQITFNGRENKIFNGIPDWVYEEEMLATKYALWWSPNG
                     KFLAYAEFNDTDIPVIAYSYYGDEQYPRTINIPYPKAGAKNPVVRIFIIDTTYPAYVG
                     PQEVPVPAMIASSDYYFSWLTWVTDERVCLQWLKRVQNVSVLSICDFREDWQTWDCPK
                     TQEHIEESRTGWAGGFFVSTPVFSYDAISYYKIFSDKDGYKHIHYIKDTVENAIQITS
                     GKWEAINIFRVTQDSLFYSSNEFEEYPGRRNIYRISIGSYPPSKKCVTCHLRKERCQY
                     YTASFSDYAKYYALVCYGPGIPISTLHDGRTDQEIKILEENKELENALKNIQLPKEEI
                     KKLEVDEITLWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVRSVFAVNWISYLASKEG
                     MVIALVDGRGTAFQGDKLLYAVYRKLGVYEVEDQITAVRKFIEMGFIDEKRIAIWGWS
                     YGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASVYTERFMGLPTKDDNLEHYKNSTV
                     MARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGLSGLS
                     TNHLYTHMTHFLKQCFSLSD"
     exon            125..209
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            210..308
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            309..403
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            404..478
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            479..531
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            532..604
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            605..725
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            726..880
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            881..984
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1120
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1121..1165
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1270
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1341
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1395
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1396..1520
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1568
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1667
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1668..1737
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1738..1932
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..1987
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1988..2087
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            2088..2152
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2225
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            2226..2299
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            2300..2696
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2672..2677
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /note="hexamer: AATAAA"
     polyA_site      2696
                     /gene="FAP"
                     /gene_synonym="DPPIV; FAPA; FAPalpha; SIMP"
                     /note="major polyA site"
ORIGIN      
        1 gtcctggctt cagcttccaa ctacaaagac agacttggtc cttttcaacg gttttcacag
       61 atccagtgac ccacgctctg aagacagaat tagctaactt tcaaaaacat ctggaaaaat
      121 gaagacttgg gtaaaaatcg tatttggagt tgccacctct gctgtgcttg ccttattggt
      181 gatgtgcatt gtcttacgcc cttcaagagt tcataactct gaagaaaata caatgagagc
      241 actcacactg aaggatattt taaatggaac attttcttat aaaacatttt ttccaaactg
      301 gatttcagga caagaatatc ttcatcaatc tgcagataac aatatagtac tttataatat
      361 tgaaacagga caatcatata ccattttgag taatagaacc atgaaaagtg tgaatgcttc
      421 aaattacggc ttatcacctg atcggcaatt tgtatatcta gaaagtgatt attcaaagct
      481 ttggagatac tcttacacag caacatatta catctatgac cttagcaatg gagaatttgt
      541 aagaggaaat gagcttcctc gtccaattca gtatttatgc tggtcgcctg ttgggagtaa
      601 attagcatat gtctatcaaa acaatatcta tttgaaacaa agaccaggag atccaccttt
      661 tcaaataaca tttaatggaa gagaaaataa aatatttaat ggaatcccag actgggttta
      721 tgaagaggaa atgcttgcta caaaatatgc tctctggtgg tctcctaatg gaaaattttt
      781 ggcatatgcg gaatttaatg atacggatat accagttatt gcctattcct attatggcga
      841 tgaacaatat cctagaacaa taaatattcc atacccaaag gctggagcta agaatcccgt
      901 tgttcggata tttattatcg ataccactta ccctgcgtat gtaggtcccc aggaagtgcc
      961 tgttccagca atgatagcct caagtgatta ttatttcagt tggctcacgt gggttactga
     1021 tgaacgagta tgtttgcagt ggctaaaaag agtccagaat gtttcggtcc tgtctatatg
     1081 tgacttcagg gaagactggc agacatggga ttgtccaaag acccaggagc atatagaaga
     1141 aagcagaact ggatgggctg gtggattctt tgtttcaaca ccagttttca gctatgatgc
     1201 catttcgtac tacaaaatat ttagtgacaa ggatggctac aaacatattc actatatcaa
     1261 agacactgtg gaaaatgcta ttcaaattac aagtggcaag tgggaggcca taaatatatt
     1321 cagagtaaca caggattcac tgttttattc tagcaatgaa tttgaagaat accctggaag
     1381 aagaaacatc tacagaatta gcattggaag ctatcctcca agcaagaagt gtgttacttg
     1441 ccatctaagg aaagaaaggt gccaatatta cacagcaagt ttcagcgact acgccaagta
     1501 ctatgcactt gtctgctacg gcccaggcat ccccatttcc acccttcatg atggacgcac
     1561 tgatcaagaa attaaaatcc tggaagaaaa caaggaattg gaaaatgctt tgaaaaatat
     1621 ccagctgcct aaagaggaaa ttaagaaact tgaagtagat gaaattactt tatggtacaa
     1681 gatgattctt cctcctcaat ttgacagatc aaagaagtat cccttgctaa ttcaagtgta
     1741 tggtggtccc tgcagtcaga gtgtaaggtc tgtatttgct gttaattgga tatcttatct
     1801 tgcaagtaag gaagggatgg tcattgcctt ggtggatggt cgaggaacag ctttccaagg
     1861 tgacaaactc ctctatgcag tgtatcgaaa gctgggtgtt tatgaagttg aagaccagat
     1921 tacagctgtc agaaaattca tagaaatggg tttcattgat gaaaaaagaa tagccatatg
     1981 gggctggtcc tatggaggat acgtttcatc actggccctt gcatctggaa ctggtctttt
     2041 caaatgtggt atagcagtgg ctccagtctc cagctgggaa tattacgcgt ctgtctacac
     2101 agagagattc atgggtctcc caacaaagga tgataatctt gagcactata agaattcaac
     2161 tgtgatggca agagcagaat atttcagaaa tgtagactat cttctcatcc acggaacagc
     2221 agatgataat gtgcactttc aaaactcagc acagattgct aaagctctgg ttaatgcaca
     2281 agtggatttc caggcaatgt ggtactctga ccagaaccac ggcttatccg gcctgtccac
     2341 gaaccactta tacacccaca tgacccactt cctaaagcag tgtttctctt tgtcagacta
     2401 aaaacgatgc agatgcaagc ctgtatcaga atctgaaaac cttatataaa cccctcagac
     2461 agtttgctta ttttattttt tatgttgtaa aatgctagta taaacaaaca aattaatgtt
     2521 gttctaaagg ctgttaaaaa aaagatgagg actcagaagt tcaagctaaa tattgtttac
     2581 attttctggt actctgtgaa agaagagaaa agggagtcat gcattttgct ttggacacag
     2641 tgttttatca cctgttcatt tgaagaaaaa taataaagtc agaagttcaa gtgcta
//
