LOCUS       NM_032130               2944 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens family with sequence similarity 186 member B
            (FAM186B), transcript variant 1, mRNA.
ACCESSION   NM_032130
VERSION     NM_032130.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB087952.1 and AL136748.1.
            
            On May 17, 2019 this sequence version replaced NM_032130.2.
            
            Summary: This gene product is a member of the FAM186 family,
            however, its exact function is not known. Alternatively spliced
            transcript variants have been found for this gene. [provided by
            RefSeq, Sep 2009].
            
            Transcript Variant: This variant (1) represents the protein coding
            transcript.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL136748.1, BC035621.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000257894.2/ ENSP00000257894.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-538               DB087952.1         14-551
            539-2944            AL136748.1         471-2876
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.12"
     gene            1..2944
                     /gene="FAM186B"
                     /gene_synonym="C12orf25"
                     /note="family with sequence similarity 186 member B"
                     /db_xref="GeneID:84070"
                     /db_xref="HGNC:HGNC:25296"
     exon            1..258
                     /gene="FAM186B"
                     /gene_synonym="C12orf25"
                     /inference="alignment:Splign:2.1.0"
     CDS             163..2844
                     /gene="FAM186B"
                     /gene_synonym="C12orf25"
                     /codon_start=1
                     /product="protein FAM186B"
                     /protein_id="NP_115506.1"
                     /db_xref="CCDS:CCDS8788.1"
                     /db_xref="GeneID:84070"
                     /db_xref="HGNC:HGNC:25296"
                     /translation="MEKDDPPQLVTPTSVKAIILRIEAAQLTRAQEDISTQLSDILDN
                     VNCVINRFQEELGYDLKENAKSQQRDPKGKKRFILLEKIASFSKDAMMKEKHLYDILR
                     WLGDWGDTLTYEIGPRKSEEEAAALDEWIEVTEKVLPLSLIATKRGIESLTALCSTLI
                     EGQKKRSQVSKRTFWQGWQGRSPQTSPSHPQPLSPEQMLQDQHTMNTKASEVTSMLQE
                     LLDSTMFSKGEVRAIRYMATVVENLNKALILQHKENRSLETKYRHLQMQATKELSSQR
                     LHFQQFMEVLESRRDALLKQVEILGGRYHDLLLMKQALEFQLKKAQNATGQAEDLAEV
                     SVDSPGPSERETLPRKETVMEESQQEPMKEEQLFSPLPPSPMAMIRDSGAIAAGHQPL
                     STMTVRSRVADVFGSKDTESLEPVLLPLVDRRFPKKWERPVAESLGHKDKDQEDYFQK
                     GGLQIKFHCSKQLSLESSRQVTSESQEEPWEEEFGREMRRQLWLEEEEMWQQRQKKWA
                     LLEQEHQEKLRQWNLEDLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRW
                     RDLEKAELSLVPAPSRTQSAHQSRRPHLPMSPSTQQPALGKQRPMSSVEFTYRPRTRR
                     VPTKPKKSASFPVTGTSIRRLTWPSLQISPANIKKKVYHMDMEAQRKNLQLLSEESEL
                     RLPHYLRSKALELTTTTMELGALRLQYLCHKYIFYRRLQSLRQEAINHVQIMKETEAS
                     YKAQNLYIFLENIDRLQSLRLQAWTDKQKGLEEKHRECLSSMVTMFPKLQLEWNVHLN
                     IPEVTSPKPKKCKLPAASPRHIRPSGPTYKQPFLSRHRACVPLQMARQQGKQMEAVWK
                     TEVASSSYAIEKKTPASLPRDQLRGHPDIPRLLTLDV"
     exon            259..484
                     /gene="FAM186B"
                     /gene_synonym="C12orf25"
                     /inference="alignment:Splign:2.1.0"
     exon            485..667
                     /gene="FAM186B"
                     /gene_synonym="C12orf25"
                     /inference="alignment:Splign:2.1.0"
     exon            668..2333
                     /gene="FAM186B"
                     /gene_synonym="C12orf25"
                     /inference="alignment:Splign:2.1.0"
     exon            2334..2526
                     /gene="FAM186B"
                     /gene_synonym="C12orf25"
                     /inference="alignment:Splign:2.1.0"
     exon            2527..2696
                     /gene="FAM186B"
                     /gene_synonym="C12orf25"
                     /inference="alignment:Splign:2.1.0"
     exon            2697..2944
                     /gene="FAM186B"
                     /gene_synonym="C12orf25"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2921..2926
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FAM186B"
                     /gene_synonym="C12orf25"
                     /note="hexamer: AATAAA"
     polyA_site      2944
                     /gene="FAM186B"
                     /gene_synonym="C12orf25"
                     /note="major polyA site"
ORIGIN      
        1 agttggaacc tggtgtttgg cagttaccag aggagctgtg cctgggcaga gggtacccag
       61 gagacaccct ggtgccctta acctccaaag caggcaacat tgtgtgtcca ggcctgtgga
      121 ccagaaacac aggatgtttt gtgactgaca gagtgatcca aaatggagaa ggatgacccc
      181 ccacagttgg tgactcccac atcagtgaaa gccatcatcc tgaggattga ggctgcccag
      241 ctaactcggg ctcaagagga tatttctacc cagctctcag acattttgga caatgtcaat
      301 tgtgtcatca accgcttcca ggaagaatta ggatatgatt taaaagaaaa tgccaaatct
      361 cagcagagag atccaaaggg caagaagaga ttcatcttgc tggaaaaaat tgcctccttc
      421 tccaaagatg ctatgatgaa ggagaagcac ctgtatgaca ttctccgctg gctgggtgac
      481 tggggtgaca ctctgaccta tgagattggg cccaggaaga gtgaagagga agcagcagct
      541 ctggacgaat ggattgaagt gacggagaaa gtgttaccgc tgtccctcat tgccaccaaa
      601 agaggcatcg agtcactcac tgccctttgc tccactctca ttgaaggaca aaagaaaagg
      661 tcacaagtgt ccaaacgcac cttctggcag ggctggcagg gaagaagccc acagacatct
      721 ccatcccatc ctcagccact aagcccagaa cagatgctcc aggaccagca taccatgaac
      781 acgaaggcct cggaggtgac gtccatgctg caggagctcc tggactctac catgttcagc
      841 aagggggagg tcagggccat caggtacatg gccactgtgg tggagaacct caacaaggcc
      901 ttgatcctcc aacacaagga gaacaggagc ctggagacca aatacaggca cctgcaaatg
      961 caggcgacca aagagctcag cagccagagg ctgcacttcc agcagttcat ggaggtcctt
     1021 gagagcagga gggatgctct gctgaagcag gtagagatct tagggggaag gtaccatgac
     1081 cttctcctga tgaagcaggc cttggagttc cagctgaaga aggctcagaa tgctacaggt
     1141 caggcagaag acctggctga ggtttctgtt gactccccag gtccctctga gagagagacc
     1201 ctcccaagga aagaaacagt catggaggaa agccaacagg aaccgatgaa ggaggagcag
     1261 ttgttctcgc cacttccccc aagtcccatg gccatgatac gggacagtgg tgctatagct
     1321 gcagggcacc agccactttc caccatgact gtgcgctcga gggtcgcaga tgtgttcggc
     1381 agcaaggaca ctgagagcct tgagcctgtg cttttaccct tagtagatcg caggtttcct
     1441 aagaaatggg aaagaccggt ggcagaaagc ttaggccaca aagacaaaga ccaggaggac
     1501 tacttccaga agggaggact ccaaattaag ttccactgta gcaagcagct gtctctagag
     1561 agctccaggc aggtgacctc tgagagccaa gaggagccct gggaggagga attcggccgg
     1621 gagatgcgga ggcagctgtg gctggaggag gaggagatgt ggcagcagcg gcagaagaag
     1681 tgggccctgc tggagcagga gcatcaggag aagctgcggc agtggaatct ggaagacctg
     1741 gccagggagc aacagcggag atgggtccag ctagaaaagg agcaggagag cccacggaga
     1801 gagccagagc agctagggga ggatgtggag aggaggatct tcacacccac cagtcgatgg
     1861 agggacttgg agaaggcaga gctatcatta gtgcctgccc caagccggac ccaatctgct
     1921 caccaaagca ggaggccaca cttgcccatg tctcctagta cccagcagcc tgccctggga
     1981 aagcagagac ctatgagttc agtggagttt acctacagac cacggacccg ccgagttccc
     2041 acaaagccca agaaatctgc ctcctttcct gtcactggga catccatccg aaggctgacc
     2101 tggccctctt tgcagatatc ccctgcaaat attaagaaga aggtgtacca catggacatg
     2161 gaggcccaga ggaagaacct gcagctcctg agtgaggagt ctgagttgag gctgccccac
     2221 tacctgcgca gcaaagcact ggagctcacc accaccacca tggagctggg cgcgctcagg
     2281 ctgcagtacc tgtgccataa gtacatcttc tatagacgcc tccagagcct ccggcaagaa
     2341 gcgatcaacc atgtacaaat catgaaagaa acggaggctt cctacaaggc ccagaacctc
     2401 tacatcttcc tggaaaacat tgaccgcctg cagagtctca ggctgcaggc ctggacggac
     2461 aagcagaagg ggctggagga gaagcaccga gagtgcctga gcagcatggt gaccatgttc
     2521 cccaagctcc agctggagtg gaacgttcac ctgaacatcc ctgaggtcac ctcgccaaag
     2581 ccaaagaaat gcaagttgcc tgcagcctca ccccggcaca tccgccccag tggccccacc
     2641 tacaagcagc cctttctgtc taggcaccgg gcatgtgtgc ccctgcagat ggcccgccaa
     2701 caggggaagc agatggaggc tgtctggaag accgaggtgg cctcctccag ttacgcaata
     2761 gaaaaaaaga cccctgccag ccttccccgg gaccagctga ggggacaccc agatattccc
     2821 cggctgttga cactggacgt gtagtcctcc tgccacaaaa gcctgaactt cctgaaggcc
     2881 cagtaaggtg cgaggctgat ggtcaatgtg gattctcccc aataaatctc tctccaggct
     2941 ttaa
//
