LOCUS       NM_000130               9132 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens coagulation factor V (F5), mRNA.
ACCESSION   NM_000130
VERSION     NM_000130.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Shimonishi,N., Morishita,E., Ogiwara,K., Maruyama,K., Yoshida,J.,
            Horie,K. and Nogami,K.
  TITLE     A novel factor V compound heterozygous mutation associated with
            thrombosis (Y1961C; FV-Kanazawa, together with 1982_1983del)
  JOURNAL   J Thromb Haemost 22 (10), 2810-2822 (2024)
   PUBMED   38950780
  REMARK    GeneRIF: A novel factor V compound heterozygous mutation associated
            with thrombosis (Y1961C; FV-Kanazawa, together with 1982_1983del).
REFERENCE   2  (bases 1 to 9132)
  AUTHORS   Lind,S.M., Sletten,M., Hellenes,M., Mathelier,A., Tekpli,X.,
            Tinholt,M. and Iversen,N.
  TITLE     Coagulation factor V in breast cancer: a p53-regulated tumor
            suppressor and predictive marker for treatment response to
            chemotherapy
  JOURNAL   J Thromb Haemost 22 (6), 1569-1582 (2024)
   PUBMED   38382738
  REMARK    GeneRIF: Coagulation factor V in breast cancer: a p53-regulated
            tumor suppressor and predictive marker for treatment response to
            chemotherapy.
REFERENCE   3  (bases 1 to 9132)
  AUTHORS   Kaneda,Y., Fukuno,K., Minami,H., Nonaka,Y., Horinoue,A., Kuroki,Y.,
            Osaki,T., Souri,M., Asakura,H. and Ichinose,A.
  TITLE     Autoimmune-acquired coagulation factor V deficiency with
            hyperfibrinolytic disseminated intravascular coagulation
  JOURNAL   Int J Lab Hematol 46 (2), 395-399 (2024)
   PUBMED   38112128
  REMARK    GeneRIF: Autoimmune-acquired coagulation factor V deficiency with
            hyperfibrinolytic disseminated intravascular coagulation.
REFERENCE   4  (bases 1 to 9132)
  AUTHORS   Al-Awadhi,A., Marouf,R., Jadaon,M.M. and Al-Awadhy,M.M.
  TITLE     Determination of vWF, ADAMTS-13 and Thrombospondin-1 in Venous
            Thromboembolism and Relating Them to the Presence of Factor V
            Leiden Mutation
  JOURNAL   Clin Appl Thromb Hemost 30, 10760296231223195 (2024)
   PUBMED   38225166
  REMARK    GeneRIF: Determination of vWF, ADAMTS-13 and Thrombospondin-1 in
            Venous Thromboembolism and Relating Them to the Presence of Factor
            V Leiden Mutation.
REFERENCE   5  (bases 1 to 9132)
  AUTHORS   Zhang,K., Ye,L., Jin,Y., Chen,Y., Jiang,S., Xie,H., Yang,L. and
            Wang,M.
  TITLE     Molecular and clinical characterization of two unrelated families
            with factor V deficiency, including a novel nonsense variant
            (p.Gln1532*)
  JOURNAL   Blood Cells Mol Dis 104, 102794 (2024)
   PUBMED   37639740
  REMARK    GeneRIF: Molecular and clinical characterization of two unrelated
            families with factor V deficiency, including a novel nonsense
            variant (p.Gln1532*).
REFERENCE   6  (bases 1 to 9132)
  AUTHORS   Pastori,D., Menichelli,D., Valeriani,E. and Pignatelli,P.
  TITLE     Factor V Leiden Thrombophilia
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301542
REFERENCE   7  (bases 1 to 9132)
  AUTHORS   Cripe,L.D., Moore,K.D. and Kane,W.H.
  TITLE     Structure of the gene for human coagulation factor V
  JOURNAL   Biochemistry 31 (15), 3777-3785 (1992)
   PUBMED   1567832
REFERENCE   8  (bases 1 to 9132)
  AUTHORS   Guglielmone,H.A. and Vides,M.A.
  TITLE     A novel functional assay of protein C in human plasma and its
            comparison with amidolytic and anticoagulant assays
  JOURNAL   Thromb Haemost 67 (1), 46-49 (1992)
   PUBMED   1615482
REFERENCE   9  (bases 1 to 9132)
  AUTHORS   Hortin,G.L.
  TITLE     Sulfation of tyrosine residues in coagulation factor V
  JOURNAL   Blood 76 (5), 946-952 (1990)
   PUBMED   2168225
REFERENCE   10 (bases 1 to 9132)
  AUTHORS   Krishnaswamy,S.
  TITLE     Prothrombinase complex assembly. Contributions of protein-protein
            and protein-membrane interactions toward complex formation
  JOURNAL   J Biol Chem 265 (7), 3708-3718 (1990)
   PUBMED   2303476
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AU279657.1, AK226079.1,
            M16967.1 and Z99572.1.
            
            On Aug 31, 2019 this sequence version replaced NM_000130.4.
            
            Summary: This gene encodes an essential cofactor of the blood
            coagulation cascade. This factor circulates in plasma, and is
            converted to the active form by the release of the activation
            peptide by thrombin during coagulation. This generates a heavy
            chain and a light chain which are held together by calcium ions.
            The activated protein is a cofactor that participates with
            activated coagulation factor X to activate prothrombin to thrombin.
            Defects in this gene result in either an autosomal recessive
            hemorrhagic diathesis or an autosomal dominant form of
            thrombophilia, which is known as activated protein C resistance.
            [provided by RefSeq, Oct 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK226079.1, M16967.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367797.9/ ENSP00000356771.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-514               AU279657.1         51-564
            515-4189            AK226079.1         483-4157
            4190-5384           M16967.1           4185-5379
            5385-6915           AK226079.1         5353-6883
            6916-9129           Z99572.1           25072-27285         c
            9130-9132           Z99572.1           25069-25071         c
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q24.2"
     gene            1..9132
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /note="coagulation factor V"
                     /db_xref="GeneID:2153"
                     /db_xref="HGNC:HGNC:3542"
                     /db_xref="MIM:612309"
     exon            1..253
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..6770
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /note="factor V Leiden; activated protein c cofactor;
                     coagulation factor V jinjiang A2 domain; coagulation
                     factor V (proaccelerin, labile factor)"
                     /codon_start=1
                     /product="coagulation factor V preproprotein"
                     /protein_id="NP_000121.2"
                     /db_xref="CCDS:CCDS1281.1"
                     /db_xref="GeneID:2153"
                     /db_xref="HGNC:HGNC:3542"
                     /db_xref="MIM:612309"
                     /translation="MFPGCPRLWVLVVLGTSWVGWGSQGTEAAQLRQFYVAAQGISWS
                     YRPEPTNSSLNLSVTSFKKIVYREYEPYFKKEKPQSTISGLLGPTLYAEVGDIIKVHF
                     KNKADKPLSIHPQGIRYSKLSEGASYLDHTFPAEKMDDAVAPGREYTYEWSISEDSGP
                     THDDPPCLTHIYYSHENLIEDFNSGLIGPLLICKKGTLTEGGTQKTFDKQIVLLFAVF
                     DESKSWSQSSSLMYTVNGYVNGTMPDITVCAHDHISWHLLGMSSGPELFSIHFNGQVL
                     EQNHHKVSAITLVSATSTTANMTVGPEGKWIISSLTPKHLQAGMQAYIDIKNCPKKTR
                     NLKKITREQRRHMKRWEYFIAAEEVIWDYAPVIPANMDKKYRSQHLDNFSNQIGKHYK
                     KVMYTQYEDESFTKHTVNPNMKEDGILGPIIRAQVRDTLKIVFKNMASRPYSIYPHGV
                     TFSPYEDEVNSSFTSGRNNTMIRAVQPGETYTYKWNILEFDEPTENDAQCLTRPYYSD
                     VDIMRDIASGLIGLLLICKSRSLDRRGIQRAADIEQQAVFAVFDENKSWYLEDNINKF
                     CENPDEVKRDDPKFYESNIMSTINGYVPESITTLGFCFDDTVQWHFCSVGTQNEILTI
                     HFTGHSFIYGKRHEDTLTLFPMRGESVTVTMDNVGTWMLTSMNSSPRSKKLRLKFRDV
                     KCIPDDDEDSYEIFEPPESTVMATRKMHDRLEPEDEESDADYDYQNRLAAALGIRSFR
                     NSSLNQEEEEFNLTALALENGTEFVSSNTDIIVGSNYSSPSNISKFTVNNLAEPQKAP
                     SHQQATTAGSPLRHLIGKNSVLNSSTAEHSSPYSEDPIEDPLQPDVTGIRLLSLGAGE
                     FKSQEHAKHKGPKVERDQAAKHRFSWMKLLAHKVGRHLSQDTGSPSGMRPWEDLPSQD
                     TGSPSRMRPWKDPPSDLLLLKQSNSSKILVGRWHLASEKGSYEIIQDTDEDTAVNNWL
                     ISPQNASRAWGESTPLANKPGKQSGHPKFPRVRHKSLQVRQDGGKSRLKKSQFLIKTR
                     KKKKEKHTHHAPLSPRTFHPLRSEAYNTFSERRLKHSLVLHKSNETSLPTDLNQTLPS
                     MDFGWIASLPDHNQNSSNDTGQASCPPGLYQTVPPEEHYQTFPIQDPDQMHSTSDPSH
                     RSSSPELSEMLEYDRSHKSFPTDISQMSPSSEHEVWQTVISPDLSQVTLSPELSQTNL
                     SPDLSHTTLSPELIQRNLSPALGQMPISPDLSHTTLSPDLSHTTLSLDLSQTNLSPEL
                     SQTNLSPALGQMPLSPDLSHTTLSLDFSQTNLSPELSHMTLSPELSQTNLSPALGQMP
                     ISPDLSHTTLSLDFSQTNLSPELSQTNLSPALGQMPLSPDPSHTTLSLDLSQTNLSPE
                     LSQTNLSPDLSEMPLFADLSQIPLTPDLDQMTLSPDLGETDLSPNFGQMSLSPDLSQV
                     TLSPDISDTTLLPDLSQISPPPDLDQIFYPSESSQSLLLQEFNESFPYPDLGQMPSPS
                     SPTLNDTFLSKEFNPLVIVGLSKDGTDYIEIIPKEEVQSSEDDYAEIDYVPYDDPYKT
                     DVRTNINSSRDPDNIAAWYLRSNNGNRRNYYIAAEEISWDYSEFVQRETDIEDSDDIP
                     EDTTYKKVVFRKYLDSTFTKRDPRGEYEEHLGILGPIIRAEVDDVIQVRFKNLASRPY
                     SLHAHGLSYEKSSEGKTYEDDSPEWFKEDNAVQPNSSYTYVWHATERSGPESPGSACR
                     AWAYYSAVNPEKDIHSGLIGPLLICQKGILHKDSNMPMDMREFVLLFMTFDEKKSWYY
                     EKKSRSSWRLTSSEMKKSHEFHAINGMIYSLPGLKMYEQEWVRLHLLNIGGSQDIHVV
                     HFHGQTLLENGNKQHQLGVWPLLPGSFKTLEMKASKPGWWLLNTEVGENQRAGMQTPF
                     LIMDRDCRMPMGLSTGIISDSQIKASEFLGYWEPRLARLNNGGSYNAWSVEKLAAEFA
                     SKPWIQVDMQKEVIITGIQTQGAKHYLKSCYTTEFYVAYSSNQINWQIFKGNSTRNVM
                     YFNGNSDASTIKENQFDPPIVARYIRISPTRAYNRPTLRLELQGCEVNGCSTPLGMEN
                     GKIENKQITASSFKKSWWGDYWEPFRARLNAQGRVNAWQAKANNNKQWLEIDLLKIKK
                     ITAIITQGCKSLSSEMYVKSYTIHYSEQGVEWKPYRLKSSMVDKIFEGNTNTKGHVKN
                     FFNPPIISRFIRVIPKTWNQSIALRLELFGCDIY"
     exon            254..345
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            346..468
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            469..681
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            682..825
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            826..1047
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1213
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1391
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1491
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1492..1706
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1857
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1858..2070
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2071..4891
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4892..5066
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5067..5303
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5304..5514
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5515..5694
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5695..5811
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5812..5883
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5884..5987
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5988..6143
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            6144..6288
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            6289..6440
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            6441..6623
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            6624..9132
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6898..6903
                     /regulatory_class="polyA_signal_sequence"
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /note="hexamer: AATAAA"
     polyA_site      6917
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /note="major polyA site"
     regulatory      9108..9113
                     /regulatory_class="polyA_signal_sequence"
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
                     /note="hexamer: AATAAA"
     polyA_site      9132
                     /gene="F5"
                     /gene_synonym="FVL; PCCF; RPRGL1; THPH2"
ORIGIN      
        1 attgcagctg ggacagcccg gagtgtggtt agcagctcgg caagcgctgc ccaggtcctg
       61 gggtggtggc agccagcggg agcaggaaag gaagcatgtt cccaggctgc ccacgcctct
      121 gggtcctggt ggtcttgggc accagctggg taggctgggg gagccaaggg acagaagcgg
      181 cacagctaag gcagttctac gtggctgctc agggcatcag ttggagctac cgacctgagc
      241 ccacaaactc aagtttgaat ctttctgtaa cttcctttaa gaaaattgtc tacagagagt
      301 atgaaccata ttttaagaaa gaaaaaccac aatctaccat ttcaggactt cttgggccta
      361 ctttatatgc tgaagtcgga gacatcataa aagttcactt taaaaataag gcagataagc
      421 ccttgagcat ccatcctcaa ggaattaggt acagtaaatt atcagaaggt gcttcttacc
      481 ttgaccacac attccctgcg gagaagatgg acgacgctgt ggctccaggc cgagaataca
      541 cctatgaatg gagtatcagt gaggacagtg gacccaccca tgatgaccct ccatgcctca
      601 cacacatcta ttactcccat gaaaatctga tcgaggattt caactcgggg ctgattgggc
      661 ccctgcttat ctgtaaaaaa gggaccctaa ctgagggtgg gacacagaag acgtttgaca
      721 agcaaatcgt gctactattt gctgtgtttg atgaaagcaa gagctggagc cagtcatcat
      781 ccctaatgta cacagtcaat ggatatgtga atgggacaat gccagatata acagtttgtg
      841 cccatgacca catcagctgg catctgctgg gaatgagctc ggggccagaa ttattctcca
      901 ttcatttcaa cggccaggtc ctggagcaga accatcataa ggtctcagcc atcacccttg
      961 tcagtgctac atccactacc gcaaatatga ctgtgggccc agagggaaag tggatcatat
     1021 cttctctcac cccaaaacat ttgcaagctg ggatgcaggc ttacattgac attaaaaact
     1081 gcccaaagaa aaccaggaat cttaagaaaa taactcgtga gcagaggcgg cacatgaaga
     1141 ggtgggaata cttcattgct gcagaggaag tcatttggga ctatgcacct gtaataccag
     1201 cgaatatgga caaaaaatac aggtctcagc atttggataa tttctcaaac caaattggaa
     1261 aacattataa gaaagttatg tacacacagt acgaagatga gtccttcacc aaacatacag
     1321 tgaatcccaa tatgaaagaa gatgggattt tgggtcctat tatcagagcc caggtcagag
     1381 acacactcaa aatcgtgttc aaaaatatgg ccagccgccc ctatagcatt taccctcatg
     1441 gagtgacctt ctcgccttat gaagatgaag tcaactcttc tttcacctca ggcaggaaca
     1501 acaccatgat cagagcagtt caaccagggg aaacctatac ttataagtgg aacatcttag
     1561 agtttgatga acccacagaa aatgatgccc agtgcttaac aagaccatac tacagtgacg
     1621 tggacatcat gagagacatc gcctctgggc taataggact acttctaatc tgtaagagca
     1681 gatccctgga caggcgagga atacagaggg cagcagacat cgaacagcag gctgtgtttg
     1741 ctgtgtttga tgagaacaaa agctggtacc ttgaggacaa catcaacaag ttttgtgaaa
     1801 atcctgatga ggtgaaacgt gatgacccca agttttatga atcaaacatc atgagcacta
     1861 tcaatggcta tgtgcctgag agcataacta ctcttggatt ctgctttgat gacactgtcc
     1921 agtggcactt ctgtagtgtg gggacccaga atgaaatttt gaccatccac ttcactgggc
     1981 actcattcat ctatggaaag aggcatgagg acaccttgac cctcttcccc atgcgtggag
     2041 aatctgtgac ggtcacaatg gataatgttg gaacttggat gttaacttcc atgaattcta
     2101 gtccaagaag caaaaagctg aggctgaaat tcagggatgt taaatgtatc ccagatgatg
     2161 atgaagactc atatgagatt tttgaacctc cagaatctac agtcatggct acacggaaaa
     2221 tgcatgatcg tttagaacct gaagatgaag agagtgatgc tgactatgat taccagaaca
     2281 gactggctgc agcattagga atcaggtcat tccgaaactc atcattgaat caggaagaag
     2341 aagagttcaa tcttactgcc ctagctctgg agaatggcac tgaattcgtt tcttcaaaca
     2401 cagatataat tgttggttca aattattctt ccccaagtaa tattagtaag ttcactgtca
     2461 ataaccttgc agaacctcag aaagcccctt ctcaccaaca agccaccaca gctggttccc
     2521 cactgagaca cctcattggc aagaactcag ttctcaattc ttccacagca gagcattcca
     2581 gcccatattc tgaagaccct atagaggatc ctctacagcc agatgtcaca gggatacgtc
     2641 tactttcact tggtgctgga gaattcaaaa gtcaagaaca tgctaagcat aagggaccca
     2701 aggtagaaag agatcaagca gcaaagcaca ggttctcctg gatgaaatta ctagcacata
     2761 aagttgggag acacctaagc caagacactg gttctccttc cggaatgagg ccctgggagg
     2821 accttcctag ccaagacact ggttctcctt ccagaatgag gccctggaag gaccctccta
     2881 gtgatctgtt actcttaaaa caaagtaact catctaagat tttggttggg agatggcatt
     2941 tggcttctga gaaaggtagc tatgaaataa tccaagatac tgatgaagac acagctgtta
     3001 acaattggct gatcagcccc cagaatgcct cacgtgcttg gggagaaagc acccctcttg
     3061 ccaacaagcc tggaaagcag agtggccacc caaagtttcc tagagttaga cataaatctc
     3121 tacaagtaag acaggatgga ggaaagagta gactgaagaa aagccagttt ctcattaaga
     3181 cacgaaaaaa gaaaaaagag aagcacacac accatgctcc tttatctccg aggacctttc
     3241 accctctaag aagtgaagcc tacaacacat tttcagaaag aagacttaag cattcgttgg
     3301 tgcttcataa atccaatgaa acatctcttc ccacagacct caatcagaca ttgccctcta
     3361 tggattttgg ctggatagcc tcacttcctg accataatca gaattcctca aatgacactg
     3421 gtcaggcaag ctgtcctcca ggtctttatc agacagtgcc cccagaggaa cactatcaaa
     3481 cattccccat tcaagaccct gatcaaatgc actctacttc agaccccagt cacagatcct
     3541 cttctccaga gctcagtgaa atgcttgagt atgaccgaag tcacaagtcc ttccccacag
     3601 atataagtca aatgtcccct tcctcagaac atgaagtctg gcagacagtc atctctccag
     3661 acctcagcca ggtgaccctc tctccagaac tcagccagac aaacctctct ccagacctca
     3721 gccacacgac tctctctcca gaactcattc agagaaacct ttccccagcc ctcggtcaga
     3781 tgcccatttc tccagacctc agccatacaa ccctttctcc agacctcagc catacaaccc
     3841 tttctttaga cctcagccag acaaacctct ctccagaact cagtcagaca aacctttctc
     3901 cagccctcgg tcagatgccc ctttctccag acctcagcca tacaaccctt tctctagact
     3961 tcagccagac aaacctctct ccagaactca gccatatgac tctctctcca gaactcagtc
     4021 agacaaacct ttccccagcc ctcggtcaga tgcccatttc tccagacctc agccatacaa
     4081 ccctttctct agacttcagc cagacaaacc tctctccaga actcagtcaa acaaaccttt
     4141 ccccagccct cggtcagatg cccctttctc cagaccccag ccatacaacc ctttctctag
     4201 acctcagcca gacaaacctc tctccagaac tcagtcagac aaacctttcc ccagacctca
     4261 gtgagatgcc cctctttgca gatctcagtc aaattcccct taccccagac ctcgaccaga
     4321 tgacactttc tccagacctt ggtgagacag atctttcccc aaactttggt cagatgtccc
     4381 tttccccaga cctcagccag gtgactctct ctccagacat cagtgacacc acccttctcc
     4441 cggatctcag ccagatatca cctcctccag accttgatca gatattctac ccttctgaat
     4501 ctagtcagtc attgcttctt caagaattta atgagtcttt tccttatcca gaccttggtc
     4561 agatgccatc tccttcatct cctactctca atgatacttt tctatcaaag gaatttaatc
     4621 cactggttat agtgggcctc agtaaagatg gtacagatta cattgagatc attccaaagg
     4681 aagaggtcca gagcagtgaa gatgactatg ctgaaattga ttatgtgccc tatgatgacc
     4741 cctacaaaac tgatgttagg acaaacatca actcctccag agatcctgac aacattgcag
     4801 catggtacct ccgcagcaac aatggaaaca gaagaaatta ttacattgct gctgaagaaa
     4861 tatcctggga ttattcagaa tttgtacaaa gggaaacaga tattgaagac tctgatgata
     4921 ttccagaaga taccacatat aagaaagtag tttttcgaaa gtacctcgac agcactttta
     4981 ccaaacgtga tcctcgaggg gagtatgaag agcatctcgg aattcttggt cctattatca
     5041 gagctgaagt ggatgatgtt atccaagttc gttttaaaaa tttagcatcc agaccgtatt
     5101 ctctacatgc ccatggactt tcctatgaaa aatcatcaga gggaaagact tatgaagatg
     5161 actctcctga atggtttaag gaagataatg ctgttcagcc aaatagcagt tatacctacg
     5221 tatggcatgc cactgagcga tcagggccag aaagtcctgg ctctgcctgt cgggcttggg
     5281 cctactactc agctgtgaac ccagaaaaag atattcactc aggcttgata ggtcccctcc
     5341 taatctgcca aaaaggaata ctacataagg acagcaacat gcctatggac atgagagaat
     5401 ttgtcttact atttatgacc tttgatgaaa agaagagctg gtactatgaa aagaagtccc
     5461 gaagttcttg gagactcaca tcctcagaaa tgaaaaaatc ccatgagttt cacgccatta
     5521 atgggatgat ctacagcttg cctggcctga aaatgtatga gcaagagtgg gtgaggttac
     5581 acctgctgaa cataggcggc tcccaagaca ttcacgtggt tcactttcac ggccagacct
     5641 tgctggaaaa tggcaataaa cagcaccagt taggggtctg gccccttctg cctggttcat
     5701 ttaaaactct tgaaatgaag gcatcaaaac ctggctggtg gctcctaaac acagaggttg
     5761 gagaaaacca gagagcaggg atgcaaacgc catttcttat catggacaga gactgtagga
     5821 tgccaatggg actaagcact ggtatcatat ctgattcaca gatcaaggct tcagagtttc
     5881 tgggttactg ggagcccaga ttagcaagat taaacaatgg tggatcttat aatgcttgga
     5941 gtgtagaaaa acttgcagca gaatttgcct ctaaaccttg gatccaggtg gacatgcaaa
     6001 aggaagtcat aatcacaggg atccagaccc aaggtgccaa acactacctg aagtcctgct
     6061 ataccacaga gttctatgta gcttacagtt ccaaccagat caactggcag atcttcaaag
     6121 ggaacagcac aaggaatgtg atgtatttta atggcaattc agatgcctct acaataaaag
     6181 agaatcagtt tgacccacct attgtggcta gatatattag gatctctcca actcgagcct
     6241 ataacagacc tacccttcga ttggaactgc aaggttgtga ggtaaatgga tgttccacac
     6301 ccctgggtat ggaaaatgga aagatagaaa acaagcaaat cacagcttct tcgtttaaga
     6361 aatcttggtg gggagattac tgggaaccct tccgtgcccg tctgaatgcc cagggacgtg
     6421 tgaatgcctg gcaagccaag gcaaacaaca ataagcagtg gctagaaatt gatctactca
     6481 agatcaagaa gataacggca attataacac agggctgcaa gtctctgtcc tctgaaatgt
     6541 atgtaaagag ctataccatc cactacagtg agcagggagt ggaatggaaa ccatacaggc
     6601 tgaaatcctc catggtggac aagatttttg aaggaaatac taataccaaa ggacatgtga
     6661 agaacttttt caacccccca atcatttcca ggtttatccg tgtcattcct aaaacatgga
     6721 atcaaagtat tgcacttcgc ctggaactct ttggctgtga tatttactag aattgaacat
     6781 tcaaaaaccc ctggaagaga ctctttaaga cctcaaacca tttagaatgg gcaatgtatt
     6841 ttacgctgtg ttaaatgtta acagttttcc actatttctc tttcttttct attagtgaat
     6901 aaaattttat acaagaagct tttataatgt aactccttgc taccagtaag taagataatg
     6961 gctattactt ctgcattaat ttgaatacag gtaggaaaat atcaagaacc aacaagaaaa
     7021 gggcttatct ttcttaatga ttgaaaatgc tatgaagtaa tatttatgta gttaaaatgc
     7081 ttcattataa ctcttttaaa tcctttacac actagtaaaa cagatattac tttaaataat
     7141 aattgataga cctggataac tttcacaaac acatgatttt ttaatggttt ttcttgagtg
     7201 aagagaaaaa caatattatc aaatgaaata agtacttaaa atatcctgtc tttcccatat
     7261 aacaatgatt tttctgactt tccatgagta aaaaaacagc caagcatctt tccagtagcc
     7321 ccattgaaat tgtgaatccg tcctggtctc cctaaggact gcacacattg atattcaagg
     7381 ttggtggtca ttagatatgg aacagaactg aaataaccat ggtagaactg aatgtgtaat
     7441 gttggcttta ttctagctgg tactacatgg cacacagttt caaaacataa tttcacctac
     7501 tggaaagctc agacctgtaa aacagagcat gggaactgct ggtctaaatg cagttgttcc
     7561 tgctcaaaga gacctctggc caaactggca agcagttaaa gttttctttc agggccttcc
     7621 tctctatggc ctcaacttcc tcctctctct tcttccagca acttcccctt tcatcattcc
     7681 tttccctggg gacttggcat tcagtgatcc tgtagatatt gcacaactgg ggaaccttta
     7741 gacatcctta aaatcacatg agatagacag tcatttgggg tgtctgaaat aaaccacccc
     7801 aaaacttagt gttaaaagag caaccaaaaa aaatttatgt gagattatgg atttgttact
     7861 tagcttgatt taatcatcct gtaacgtgta catatatcaa aatgttatgt ataccataaa
     7921 tatataaaat tttatcaacg aaattcataa caatctctca gaccacagag aaatcaaatt
     7981 agaactgagg actaagaaac tcactcgaaa ccacacaact acatggaaac tgaacaacct
     8041 gctcctgaat gactactggg taaataatga aattaaggca gaaataaata agttccttaa
     8101 aaccaatgag aacaaagaga caacatacca gaatctctag gagacagggc tttgcttttg
     8161 ctgcattcta ttcgttgtga acacaaatta caggccagtc tcgattcagt gtagaaggga
     8221 actgcataag gaccacatac caggaggcat aattcactgg gagcatcttt agaaactacc
     8281 agagttacct gttgcccata ccagtggggt aagccctatg aatgtatatg agagtttcaa
     8341 acatccacaa aacattggct ttctaatatt cgtattccca ctattccttt cttttcatga
     8401 ttcatgtcat tgtcccatca acatttctaa gatttccatt ccgttaagag caaaagagaa
     8461 tgttggaagg tgggggaaaa catttctttg ttttctacag ggccagcttc ttggatgtgt
     8521 gtgatctgtt cagttgcaaa gggtcacatg ctcagaagga ccgcatgcta aatttaatgc
     8581 tttgcagtta ccctcttgaa atcctttatt ttttaagaag gaattcgaca tttccatttt
     8641 tcaatgagcc ccacaaatta cgcagctagt cctgggcttc tctactctga aattgggcag
     8701 gatctctctt gatctagaat ttactaaggc ataatagggg caagaaaatc ttatgaaata
     8761 atggggggta gggaagagat gggaatggag catgagatcc agcttcgtta ttctctactt
     8821 gagaaaaata aggccccaaa gattaaacaa cttgcccaag gatattgctt gttagtgtca
     8881 gaactgaaac cagaaaccaa atgatcatat ccctagactt ttagtctgct ttctcttcca
     8941 taaaatgaaa cttataatgt ttctaatcca ttgctcagac aggtagacat gaatattaat
     9001 tgataatgac tattaattga tctggaaaat acttgtttgg ggatcaataa tatgtttggg
     9061 ctattatcta atgctgtgta gaaatattaa aacccctgtt attttgaaat aaaaaagata
     9121 cccacttttt at
//
