LOCUS       NM_001178096            2138 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens coagulation factor III, tissue factor (F3), transcript
            variant 2, mRNA.
ACCESSION   NM_001178096
VERSION     NM_001178096.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Zhang,Y., Liu,L., Pei,J., Ren,Z., Deng,Y. and Yu,K.
  TITLE     Tissue factor overexpression promotes resistance to KRAS-G12C
            inhibition in non-small cell lung cancer
  JOURNAL   Oncogene 43 (9), 668-681 (2024)
   PUBMED   38191673
  REMARK    GeneRIF: Tissue factor overexpression promotes resistance to
            KRAS-G12C inhibition in non-small cell lung cancer.
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Eichhorn,T., Weiss,R., Huber,S., Ebeyer-Masotta,M., Mostageer,M.,
            Emprechtinger,R., Knabl,L. Sr., Knabl,L., Wurzner,R. and Weber,V.
  TITLE     Expression of Tissue Factor and Platelet/Leukocyte Markers on
            Extracellular Vesicles Reflect Platelet-Leukocyte Interaction in
            Severe COVID-19
  JOURNAL   Int J Mol Sci 24 (23), 16886 (2023)
   PUBMED   38069209
  REMARK    GeneRIF: Expression of Tissue Factor and Platelet/Leukocyte Markers
            on Extracellular Vesicles Reflect Platelet-Leukocyte Interaction in
            Severe COVID-19.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2138)
  AUTHORS   Brambilla,M., Becchetti,A., Rovati,G.E., Cosentino,N., Conti,M.,
            Canzano,P., Giesen,P.L.A., Loffreda,A., Bonomi,A., Cattaneo,M., De
            Candia,E., Podda,G.M., Trabattoni,D., Werba,P.J., Campodonico,J.,
            Pinna,C., Marenzi,G., Tremoli,E. and Camera,M.
  TITLE     Cell Surface Platelet Tissue Factor Expression: Regulation by P2Y12
            and Link to Residual Platelet Reactivity
  JOURNAL   Arterioscler Thromb Vasc Biol 43 (10), 2042-2057 (2023)
   PUBMED   37589138
  REMARK    GeneRIF: Cell Surface Platelet Tissue Factor Expression: Regulation
            by P2Y12 and Link to Residual Platelet Reactivity.
REFERENCE   4  (bases 1 to 2138)
  AUTHORS   Zioncheck,T.F., Roy,S. and Vehar,G.A.
  TITLE     The cytoplasmic domain of tissue factor is phosphorylated by a
            protein kinase C-dependent mechanism
  JOURNAL   J Biol Chem 267 (6), 3561-3564 (1992)
   PUBMED   1740409
REFERENCE   5  (bases 1 to 2138)
  AUTHORS   Broze,G.J. Jr., Girard,T.J. and Novotny,W.F.
  TITLE     Regulation of coagulation by a multivalent Kunitz-type inhibitor
  JOURNAL   Biochemistry 29 (33), 7539-7546 (1990)
   PUBMED   2271516
  REMARK    Review article
REFERENCE   6  (bases 1 to 2138)
  AUTHORS   Mackman,N., Morrissey,J.H., Fowler,B. and Edgington,T.S.
  TITLE     Complete sequence of the human tissue factor gene, a highly
            regulated cellular receptor that initiates the coagulation protease
            cascade
  JOURNAL   Biochemistry 28 (4), 1755-1762 (1989)
   PUBMED   2719931
REFERENCE   7  (bases 1 to 2138)
  AUTHORS   Kao,F.T., Hartz,J., Horton,R., Nemerson,Y. and Carson,S.D.
  TITLE     Regional assignment of human tissue factor gene (F3) to chromosome
            1p21-p22
  JOURNAL   Somat Cell Mol Genet 14 (4), 407-410 (1988)
   PUBMED   3399965
REFERENCE   8  (bases 1 to 2138)
  AUTHORS   Scarpati,E.M., Wen,D., Broze,G.J. Jr., Miletich,J.P.,
            Flandermeyer,R.R., Siegel,N.R. and Sadler,J.E.
  TITLE     Human tissue factor: cDNA sequence and chromosome localization of
            the gene
  JOURNAL   Biochemistry 26 (17), 5234-5238 (1987)
   PUBMED   2823875
REFERENCE   9  (bases 1 to 2138)
  AUTHORS   Spicer,E.K., Horton,R., Bloem,L., Bach,R., Williams,K.R., Guha,A.,
            Kraus,J., Lin,T.C., Nemerson,Y. and Konigsberg,W.H.
  TITLE     Isolation of cDNA clones coding for human tissue factor: primary
            structure of the protein and cDNA
  JOURNAL   Proc Natl Acad Sci U S A 84 (15), 5148-5152 (1987)
   PUBMED   3037536
REFERENCE   10 (bases 1 to 2138)
  AUTHORS   Gouault-Helimann,M. and Josso,F.
  TITLE     [Initiation in vivo of blood coagulation. The role of white blood
            cells and tissue factor (author's transl)]
  JOURNAL   Nouv Presse Med 8 (40), 3249-3253 (1979)
   PUBMED   392457
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB499288.1, AF487337.1 and
            AC093117.2.
            
            On Aug 13, 2020 this sequence version replaced NM_001178096.1.
            
            Summary: This gene encodes coagulation factor III which is a cell
            surface glycoprotein. This factor enables cells to initiate the
            blood coagulation cascades, and it functions as the high-affinity
            receptor for the coagulation factor VII. The resulting complex
            provides a catalytic event that is responsible for initiation of
            the coagulation protease cascades by specific limited proteolysis.
            Unlike the other cofactors of these protease cascades, which
            circulate as nonfunctional precursors, this factor is a potent
            initiator that is fully functional when expressed on cell surfaces,
            for example, on monocytes. There are 3 distinct domains of this
            factor: extracellular, transmembrane, and cytoplasmic. Platelets
            and monocytes have been shown to express this coagulation factor
            under procoagulatory and proinflammatory stimuli, and a major role
            in HIV-associated coagulopathy has been described.
            Platelet-dependent monocyte expression of coagulation factor III
            has been described to be associated with Coronavirus Disease 2019
            (COVID-19) severity and mortality. This protein is the only one in
            the coagulation pathway for which a congenital deficiency has not
            been described. Alternate splicing results in multiple transcript
            variants.[provided by RefSeq, Aug 2020].
            
            Transcript Variant: This variant (2) lacks an exon in the coding
            region, which results in a frameshift and an early stop codon,
            compared to variant 1. The encoded isoform (2) is shorter and has a
            distinct C-terminus, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF497569.1, AF497570.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related :: relevant for disease process
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-122               DB499288.1         101-222
            123-852             AF487337.1         1-730
            853-2138            AC093117.2         14463-15748         c
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p21.3"
     gene            1..2138
                     /gene="F3"
                     /gene_synonym="CD142; TF; TFA"
                     /note="coagulation factor III, tissue factor"
                     /db_xref="GeneID:2152"
                     /db_xref="HGNC:HGNC:3541"
                     /db_xref="MIM:134390"
     exon            1..223
                     /gene="F3"
                     /gene_synonym="CD142; TF; TFA"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..840
                     /gene="F3"
                     /gene_synonym="CD142; TF; TFA"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; coagulation factor III (thromboplastin, tissue
                     factor)"
                     /codon_start=1
                     /product="tissue factor isoform 2 precursor"
                     /protein_id="NP_001171567.1"
                     /db_xref="CCDS:CCDS53345.1"
                     /db_xref="GeneID:2152"
                     /db_xref="HGNC:HGNC:3541"
                     /db_xref="MIM:134390"
                     /translation="METPAWPRVPRPETAVARTLLLGWVFAQVAGASGTTNTVAAYNL
                     TWKSTNFKTILEWEPKPVNQVYTVQISTKSGDWKSKCFYTTDTECDLTDEIVKDVKQT
                     YLARVFSYPAGNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVE
                     DERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKYSTSLELWYLWSSSLSSSWL
                     YLYTSVERQEWGRAGRRTPH"
     exon            224..335
                     /gene="F3"
                     /gene_synonym="CD142; TF; TFA"
                     /inference="alignment:Splign:2.1.0"
     exon            336..535
                     /gene="F3"
                     /gene_synonym="CD142; TF; TFA"
                     /inference="alignment:Splign:2.1.0"
     exon            536..714
                     /gene="F3"
                     /gene_synonym="CD142; TF; TFA"
                     /inference="alignment:Splign:2.1.0"
     exon            715..2138
                     /gene="F3"
                     /gene_synonym="CD142; TF; TFA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1972..1977
                     /regulatory_class="polyA_signal_sequence"
                     /gene="F3"
                     /gene_synonym="CD142; TF; TFA"
                     /note="hexamer: AATAAA"
     polyA_site      1995
                     /gene="F3"
                     /gene_synonym="CD142; TF; TFA"
                     /note="major polyA site"
     regulatory      2064..2069
                     /regulatory_class="polyA_signal_sequence"
                     /gene="F3"
                     /gene_synonym="CD142; TF; TFA"
                     /note="hexamer: AGTAAA"
     polyA_site      2086
                     /gene="F3"
                     /gene_synonym="CD142; TF; TFA"
     regulatory      2119..2124
                     /regulatory_class="polyA_signal_sequence"
                     /gene="F3"
                     /gene_synonym="CD142; TF; TFA"
                     /note="hexamer: AATACA"
     polyA_site      2138
                     /gene="F3"
                     /gene_synonym="CD142; TF; TFA"
ORIGIN      
        1 aagactgcga gctccccgca ccccctcgca ctccctctgg ccggcccagg gcgccttcag
       61 cccaacctcc ccagccccac gggcgccacg gaacccgctc gatctcgccg ccaactggta
      121 gacatggaga cccctgcctg gccccgggtc ccgcgccccg agaccgccgt cgctcggacg
      181 ctcctgctcg gctgggtctt cgcccaggtg gccggcgctt caggcactac aaatactgtg
      241 gcagcatata atttaacttg gaaatcaact aatttcaaga caattttgga gtgggaaccc
      301 aaacccgtca atcaagtcta cactgttcaa ataagcacta agtcaggaga ttggaaaagc
      361 aaatgctttt acacaacaga cacagagtgt gacctcaccg acgagattgt gaaggatgtg
      421 aagcagacgt acttggcacg ggtcttctcc tacccggcag ggaatgtgga gagcaccggt
      481 tctgctgggg agcctctgta tgagaactcc ccagagttca caccttacct ggagacaaac
      541 ctcggacagc caacaattca gagttttgaa caggtgggaa caaaagtgaa tgtgaccgta
      601 gaagatgaac ggactttagt cagaaggaac aacactttcc taagcctccg ggatgttttt
      661 ggcaaggact taatttatac actttattat tggaaatctt caagttcagg aaagaaatat
      721 tctacatcat tggagctgtg gtatttgtgg tcatcatcct tgtcatcatc ctggctatat
      781 ctctacacaa gtgtagaaag gcaggagtgg ggcagagctg gaaggagaac tccccactga
      841 atgtttcata aaggaagcac tgttggagct actgcaaatg ctatattgca ctgtgaccga
      901 gaacttttaa gaggatagaa tacatggaaa cgcaaatgag tatttcggag catgaagacc
      961 ctggagttca aaaaactctt gatatgacct gttattacca ttagcattct ggttttgaca
     1021 tcagcattag tcactttgaa atgtaacgaa tggtactaca accaattcca agttttaatt
     1081 tttaacacca tggcaccttt tgcacataac atgctttaga ttatatattc cgcactcaag
     1141 gagtaaccag gtcgtccaag caaaaacaaa tgggaaaatg tcttaaaaaa tcctgggtgg
     1201 acttttgaaa agcttttttt tttttttttt tttttttgag acggagtctt gctctgttgc
     1261 ccaggctgga gtgcagtagc acgatctcgg ctcactgcac cctccgtctc tcgggttcaa
     1321 gcaattgtct gcctcagcct cccgagtagc tgggattaca ggtgcgcact accacgccaa
     1381 gctaattttt gtatttttta gtagagatgg ggtttcacca tcttggccag gctggtcttg
     1441 aattcctgac ctcaggtgat ccacccacct tggcctccca aagtgctagt attatgggcg
     1501 tgaaccacca tgcccagccg aaaagctttt gaggggctga cttcaatcca tgtaggaaag
     1561 taaaatggaa ggaaattggg tgcatttcta ggacttttct aacatatgtc tataatatag
     1621 tgtttaggtt cttttttttt tcaggaatac atttggaaat tcaaaacaat tggcaaactt
     1681 tgtattaatg tgttaagtgc aggagacatt ggtattctgg gcaccttcct aatatgcttt
     1741 acaatctgca ctttaactga cttaagtggc attaaacatt tgagagctaa ctatattttt
     1801 ataagactac tatacaaact acagagttta tgatttaagg tacttaaagc ttctatggtt
     1861 gacattgtat atataatttt ttaaaaaggt tttctatatg gggattttct atttatgtag
     1921 gtaatattgt tctatttgta tatattgaga taatttattt aatatacttt aaataaaggt
     1981 gactgggaat tgttactgtt gtacttattc tatcttccat ttattattta tgtacaattt
     2041 ggtgtttgta ttagctctac tacagtaaat gactgtaaaa ttgtcagtgg cttacaacaa
     2101 cgtatctttt tcgcttataa tacattttgg tgactgta
//
