LOCUS       NM_004101               3398 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens coagulation factor II thrombin receptor like 2
            (F2RL2), transcript variant 1, mRNA.
ACCESSION   NM_004101
VERSION     NM_004101.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Tillery,L.C., Epperson,T.A., Eguchi,S. and Motley,E.D.
  TITLE     Featured Article: Differential regulation of endothelial nitric
            oxide synthase phosphorylation by protease-activated receptors in
            adult human endothelial cells
  JOURNAL   Exp Biol Med (Maywood) 241 (6), 569-580 (2016)
   PUBMED   26729042
  REMARK    GeneRIF: TFRGAP, a PAR-3 mimicking peptide significantly induced
            the phosphorylation of eNOS-Thr-495 with minimal phosphorylation of
            eNOS-Ser-1177 with no change in nitric oxide production.
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Hanzelmann,S., Wang,J., Guney,E., Tang,Y., Zhang,E., Axelsson,A.S.,
            Nenonen,H., Salehi,A.S., Wollheim,C.B., Zetterberg,E., Berntorp,E.,
            Costa,I.G., Castelo,R. and Rosengren,A.H.
  TITLE     Thrombin stimulates insulin secretion via protease-activated
            receptor-3
  JOURNAL   Islets 7 (4), e1118195 (2015)
   PUBMED   26742564
  REMARK    GeneRIF: PAR3 is essential in thrombin stimulated insulin
            secretion.
REFERENCE   3  (bases 1 to 3398)
  AUTHORS   Stavenuiter,F. and Mosnier,L.O.
  TITLE     Noncanonical PAR3 activation by factor Xa identifies a novel
            pathway for Tie2 activation and stabilization of vascular integrity
  JOURNAL   Blood 124 (23), 3480-3489 (2014)
   PUBMED   25320242
  REMARK    GeneRIF: These data provide intriguing novel insights into the
            diversification of functional selectivity of protease signaling
            achievable by canonical and noncanonical PAR activation, such as
            the activation of vascular-protective Tie2 by noncanonical PAR3
            activation.
REFERENCE   4  (bases 1 to 3398)
  AUTHORS   Segal,L., Katz,L.S., Shapira,H., Sandbank,J., Geras-Raaka,E.,
            Gershengorn,M.C. and Oron,Y.
  TITLE     PAR-3 knockdown enhances adhesion rate of PANC-1 cells via
            increased expression of integrinalphav and E-cadherin
  JOURNAL   PLoS One 9 (4), e93879 (2014)
   PUBMED   24699825
  REMARK    GeneRIF: In pancreatic adenocarcinoma cells, PAR3 knockdown
            enhances cell adhesion. We propose this is due to increased
            expression of E-cadherin, leading to a greater adhesion of
            free-floating cells to cells bound to the surface via integrins,
            particularly ITGalphav.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3398)
  AUTHORS   Guyonnet Duperat,V., Jacquelin,B., Boisseau,P., Arveiler,B. and
            Nurden,A.T.
  TITLE     Protease-activated receptor genes are clustered on 5q13
  JOURNAL   Blood 92 (1), 25-31 (1998)
   PUBMED   9639495
REFERENCE   6  (bases 1 to 3398)
  AUTHORS   Schmidt,V.A., Nierman,W.C., Maglott,D.R., Cupit,L.D.,
            Moskowitz,K.A., Wainer,J.A. and Bahou,W.F.
  TITLE     The human proteinase-activated receptor-3 (PAR-3) gene.
            Identification within a Par gene cluster and characterization in
            vascular endothelial cells and platelets
  JOURNAL   J Biol Chem 273 (24), 15061-15068 (1998)
   PUBMED   9614115
REFERENCE   7  (bases 1 to 3398)
  AUTHORS   Xu,W.F., Andersen,H., Whitmore,T.E., Presnell,S.R., Yee,D.P.,
            Ching,A., Gilbert,T., Davie,E.W. and Foster,D.C.
  TITLE     Cloning and characterization of human protease-activated receptor 4
  JOURNAL   Proc Natl Acad Sci U S A 95 (12), 6642-6646 (1998)
   PUBMED   9618465
REFERENCE   8  (bases 1 to 3398)
  AUTHORS   Ishihara,H., Connolly,A.J., Zeng,D., Kahn,M.L., Zheng,Y.W.,
            Timmons,C., Tram,T. and Coughlin,S.R.
  TITLE     Protease-activated receptor 3 is a second thrombin receptor in
            humans
  JOURNAL   Nature 386 (6624), 502-506 (1997)
   PUBMED   9087410
REFERENCE   9  (bases 1 to 3398)
  AUTHORS   Offermanns,S., Laugwitz,K.L., Spicher,K. and Schultz,G.
  TITLE     G proteins of the G12 family are activated via thromboxane A2 and
            thrombin receptors in human platelets
  JOURNAL   Proc Natl Acad Sci U S A 91 (2), 504-508 (1994)
   PUBMED   8290554
REFERENCE   10 (bases 1 to 3398)
  AUTHORS   Shenker,A., Goldsmith,P., Unson,C.G. and Spiegel,A.M.
  TITLE     The G protein coupled to the thromboxane A2 receptor in human
            platelets is a member of the novel Gq family
  JOURNAL   J Biol Chem 266 (14), 9309-9313 (1991)
   PUBMED   1851174
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC419029.1, BC093650.1,
            AC026725.7 and AL048674.3.
            
            On Nov 23, 2018 this sequence version replaced NM_004101.3.
            
            Summary: This gene encodes a member of the protease-activated
            receptor (PAR) family which is a subfamily of the seven
            transmembrane G protein-coupled cell surface receptor family. The
            encoded protein acts as a cofactor in the thrombin-mediated
            cleavage and activation of the protease-activated receptor family
            member PAR4. The encoded protein plays an essential role in
            hemostasis and thrombosis. Alternate splicing results in multiple
            transcript variants that encode different isoforms. [provided by
            RefSeq, Feb 2012].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DA578877.1, BX537386.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000296641.5/ ENSP00000296641.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-74                DC419029.1         32-105
            75-1388             BC093650.1         1-1314
            1389-3204           AC026725.7         90510-92325         c
            3205-3398           AL048674.3         1-194
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q13.3"
     gene            1..3398
                     /gene="F2RL2"
                     /gene_synonym="PAR-3; PAR3"
                     /note="coagulation factor II thrombin receptor like 2"
                     /db_xref="GeneID:2151"
                     /db_xref="HGNC:HGNC:3539"
                     /db_xref="MIM:601919"
     exon            1..237
                     /gene="F2RL2"
                     /gene_synonym="PAR-3; PAR3"
                     /inference="alignment:Splign:2.1.0"
     CDS             174..1298
                     /gene="F2RL2"
                     /gene_synonym="PAR-3; PAR3"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; protease-activated receptor 3; Coagulation
                     factor II receptor-like 2 (protease-actovated receptor 3);
                     proteinase-activated receptor 3; thrombin receptor-like 2"
                     /codon_start=1
                     /product="proteinase-activated receptor 3 isoform 1
                     precursor"
                     /protein_id="NP_004092.1"
                     /db_xref="CCDS:CCDS4031.1"
                     /db_xref="GeneID:2151"
                     /db_xref="HGNC:HGNC:3539"
                     /db_xref="MIM:601919"
                     /translation="MKALIFAAAGLLLLLPTFCQSGMENDTNNLAKPTLPIKTFRGAP
                     PNSFEEFPFSALEGWTGATITVKIKCPEESASHLHVKNATMGYLTSSLSTKLIPAIYL
                     LVFVVGVPANAVTLWMLFFRTRSICTTVFYTNLAIADFLFCVTLPFKIAYHLNGNNWV
                     FGEVLCRATTVIFYGNMYCSILLLACISINRYLAIVHPFTYRGLPKHTYALVTCGLVW
                     ATVFLYMLPFFILKQEYYLVQPDITTCHDVHNTCESSSPFQLYYFISLAFFGFLIPFV
                     LIIYCYAAIIRTLNAYDHRWLWYVKASLLILVIFTICFAPSNIILIIHHANYYYNNTD
                     GLYFIYLIALCLGSLNSCLDPFLYFLMSKTRNHSTAYLTK"
     exon            238..3398
                     /gene="F2RL2"
                     /gene_synonym="PAR-3; PAR3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3376..3381
                     /regulatory_class="polyA_signal_sequence"
                     /gene="F2RL2"
                     /gene_synonym="PAR-3; PAR3"
                     /note="hexamer: AATAAA"
     polyA_site      3398
                     /gene="F2RL2"
                     /gene_synonym="PAR-3; PAR3"
                     /note="major polyA site"
ORIGIN      
        1 ctttccctgc ggaggaccag ggcaagtttc ctgcctgcac ggcacaggag agcaaacttc
       61 tacagacaga ccaaggcttc catttgctgc tgacacatgg aactgaggtg aaattgtgct
      121 ccatgatttt acagatttca taacgtttaa gagacgggac tcaggtcatc aaaatgaaag
      181 ccctcatctt tgcagctgct ggcctcctgc ttctgttgcc cactttttgt cagagtggca
      241 tggaaaatga tacaaacaac ttggcaaagc caaccttacc cattaagacc tttcgtggag
      301 ctcccccaaa ttcttttgaa gagttcccct tttctgcctt ggaaggctgg acaggagcca
      361 cgattactgt aaaaattaag tgccctgaag aaagtgcttc acatctccat gtgaaaaatg
      421 ctaccatggg gtacctgacc agctccttaa gtactaaact gatacctgcc atctacctcc
      481 tggtgtttgt agttggtgtc ccggccaatg ctgtgaccct gtggatgctt ttcttcagga
      541 ccagatccat ctgtaccact gtattctaca ccaacctggc cattgcagat tttctttttt
      601 gtgttacatt gccctttaag atagcttatc atctcaatgg gaacaactgg gtatttggag
      661 aggtcctgtg ccgggccacc acagtcatct tctatggcaa catgtactgc tccattctgc
      721 tccttgcctg catcagcatc aaccgctacc tggccatcgt ccatcctttc acctaccggg
      781 gcctgcccaa gcacacctat gccttggtaa catgtggact ggtgtgggca acagttttct
      841 tatatatgct gccatttttc atactgaagc aggaatatta tcttgttcag ccagacatca
      901 ccacctgcca tgatgttcac aacacttgcg agtcctcatc tcccttccaa ctctattact
      961 tcatctcctt ggcattcttt ggattcttaa ttccatttgt gcttatcatc tactgctatg
     1021 cagccatcat ccggacactt aatgcatacg atcatagatg gttgtggtat gttaaggcga
     1081 gtctcctcat ccttgtgatt tttaccattt gctttgctcc aagcaatatt attcttatta
     1141 ttcaccatgc taactactac tacaacaaca ctgatggctt atattttata tatctcatag
     1201 ctttgtgcct gggtagtctt aatagttgct tagatccatt cctttatttt ctcatgtcaa
     1261 aaaccagaaa tcactccact gcttacctta caaaatagtg aaatgatctt agagaacaag
     1321 gacagccatc acagagaacg tctgttttca agaacaacat aagcatagtg caaggagctc
     1381 catttccgag ctcctaagaa atatgcttca aaggtcaaac attacaaaag cattagtagt
     1441 ttgtttgttt gtttttgaga ctgagtctca ctttatcacc cagactggcg tgcagtggca
     1501 ctatcttggc tcattgcaac ctctgcctcc caggtcagcc tcccaagtag ctgggattac
     1561 accaccatgc ccagctacta aaaatacttg tatttttagt agagacgggg tttcaccatg
     1621 ttgaccaggc tggtcttgaa ctcctgacct caagtgatct tccggcctca gcctcccaaa
     1681 gtgctggatt acaggcgtga gccactgagc cagccagcat tagtaatttt taaaaacact
     1741 ttatcagtat tttaaaaatg ttaatgcagg agaaaagata tcacaactct atggaaaatg
     1801 acatttccat ttgccttatt gctacttcaa gctctttaaa tcaccatctt ccctatttct
     1861 gtgagtggta ttgccatcct tgacatttga catcattttt tattctttgg tctcttttga
     1921 ctctcatgct ggtggctgcc tcatcaactg attctatctt tgtagggtcc ctcaccaggg
     1981 tcttttatct agtttcacct ttgccttctt ttttcctttt tttaatagag acagtgtctc
     2041 gcttcgtcac ctggctgaag tgcagtagcc tgatcatagc tcacttcagc ctccaactcc
     2101 tgggctcaag caatcctgct gtctcagcct cccaagtaac taagactaca ggcatgcacc
     2161 accataccca actaatttta ttttttattt tttgcagaga tggagtctca ctgttgccca
     2221 ggctggtctt gaactcctgg tctcgagtga tcttcctatc tcagcctccc aaagtgctgg
     2281 gactacaggc atgagccact acgtccaggc cacctctacc ttctaaccat tctctccact
     2341 gccagcctac cctccaccac ttcacaagtc ctgccagatt aatcttcctt agatatcagt
     2401 taggatacat cactaaaaca tttccactaa ctccactgtt tctataataa atcataaatc
     2461 tttagcctgg cattcaaggc taacttgcta atcacacttc ccattaaacc ttgcttatga
     2521 tataggcacc aaccaaacct cttgctctgc tatatctcca ctctgcctca gactgaaaag
     2581 ctagtcctaa cgtctttgcc ttaattctgt tcattttttc aagtccttga aggaccattc
     2641 ttccctgatt ctcaggctag aagtgtcact tttcttatct gtacttccaa agcactttcg
     2701 tatattttta ttatggcatt tatatatagt tcatttatat ttaaatttta attccatgaa
     2761 caatcaagta ccaagtataa tggagaaggt gctcatcctc tgccttcctt gagcttctgg
     2821 gtgatgccag gcccaagtct ttgtggcacc cagctccatg ctttgaatac tatgtggctg
     2881 aatgaatttt taaaatctca aagcagttaa acagcaggaa agcccattaa cttcgtactg
     2941 aaaaagcaac atactgtgat gatacgggat gacatcattt caggttgggc atacaaaaaa
     3001 gtaaggaagc taaactaaga ctatactcac caggccattt agaagtttta aataatgcct
     3061 ccactatttt ttttcttaga catagctttt aatggggaaa tggaattttg ttattaatac
     3121 ccattatatt cctgtaagta aatgactatt tttcccttaa ctcagtgatt agacaggaag
     3181 gaagacatta gtgattagac aggagggaag atattagtga ttagacagca gggaagatat
     3241 tagtggtaaa gagtgaatga tagtagtgaa tataaatggg gctgaggaaa ctttaagcat
     3301 aaaagattcc tgagatgact ttacaagtct gtacgaatct gccttgactg tatatttcat
     3361 actgcccaac aaaacaataa aaaacaattt taaatgca
//
