LOCUS       NM_001256566            3268 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens coagulation factor II thrombin receptor like 2
            (F2RL2), transcript variant 2, mRNA.
ACCESSION   NM_001256566
VERSION     NM_001256566.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Tillery,L.C., Epperson,T.A., Eguchi,S. and Motley,E.D.
  TITLE     Featured Article: Differential regulation of endothelial nitric
            oxide synthase phosphorylation by protease-activated receptors in
            adult human endothelial cells
  JOURNAL   Exp Biol Med (Maywood) 241 (6), 569-580 (2016)
   PUBMED   26729042
  REMARK    GeneRIF: TFRGAP, a PAR-3 mimicking peptide significantly induced
            the phosphorylation of eNOS-Thr-495 with minimal phosphorylation of
            eNOS-Ser-1177 with no change in nitric oxide production.
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Hanzelmann,S., Wang,J., Guney,E., Tang,Y., Zhang,E., Axelsson,A.S.,
            Nenonen,H., Salehi,A.S., Wollheim,C.B., Zetterberg,E., Berntorp,E.,
            Costa,I.G., Castelo,R. and Rosengren,A.H.
  TITLE     Thrombin stimulates insulin secretion via protease-activated
            receptor-3
  JOURNAL   Islets 7 (4), e1118195 (2015)
   PUBMED   26742564
  REMARK    GeneRIF: PAR3 is essential in thrombin stimulated insulin
            secretion.
REFERENCE   3  (bases 1 to 3268)
  AUTHORS   Stavenuiter,F. and Mosnier,L.O.
  TITLE     Noncanonical PAR3 activation by factor Xa identifies a novel
            pathway for Tie2 activation and stabilization of vascular integrity
  JOURNAL   Blood 124 (23), 3480-3489 (2014)
   PUBMED   25320242
  REMARK    GeneRIF: These data provide intriguing novel insights into the
            diversification of functional selectivity of protease signaling
            achievable by canonical and noncanonical PAR activation, such as
            the activation of vascular-protective Tie2 by noncanonical PAR3
            activation.
REFERENCE   4  (bases 1 to 3268)
  AUTHORS   Segal,L., Katz,L.S., Shapira,H., Sandbank,J., Geras-Raaka,E.,
            Gershengorn,M.C. and Oron,Y.
  TITLE     PAR-3 knockdown enhances adhesion rate of PANC-1 cells via
            increased expression of integrinalphav and E-cadherin
  JOURNAL   PLoS One 9 (4), e93879 (2014)
   PUBMED   24699825
  REMARK    GeneRIF: In pancreatic adenocarcinoma cells, PAR3 knockdown
            enhances cell adhesion. We propose this is due to increased
            expression of E-cadherin, leading to a greater adhesion of
            free-floating cells to cells bound to the surface via integrins,
            particularly ITGalphav.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3268)
  AUTHORS   Guyonnet Duperat,V., Jacquelin,B., Boisseau,P., Arveiler,B. and
            Nurden,A.T.
  TITLE     Protease-activated receptor genes are clustered on 5q13
  JOURNAL   Blood 92 (1), 25-31 (1998)
   PUBMED   9639495
REFERENCE   6  (bases 1 to 3268)
  AUTHORS   Schmidt,V.A., Nierman,W.C., Maglott,D.R., Cupit,L.D.,
            Moskowitz,K.A., Wainer,J.A. and Bahou,W.F.
  TITLE     The human proteinase-activated receptor-3 (PAR-3) gene.
            Identification within a Par gene cluster and characterization in
            vascular endothelial cells and platelets
  JOURNAL   J Biol Chem 273 (24), 15061-15068 (1998)
   PUBMED   9614115
REFERENCE   7  (bases 1 to 3268)
  AUTHORS   Xu,W.F., Andersen,H., Whitmore,T.E., Presnell,S.R., Yee,D.P.,
            Ching,A., Gilbert,T., Davie,E.W. and Foster,D.C.
  TITLE     Cloning and characterization of human protease-activated receptor 4
  JOURNAL   Proc Natl Acad Sci U S A 95 (12), 6642-6646 (1998)
   PUBMED   9618465
REFERENCE   8  (bases 1 to 3268)
  AUTHORS   Ishihara,H., Connolly,A.J., Zeng,D., Kahn,M.L., Zheng,Y.W.,
            Timmons,C., Tram,T. and Coughlin,S.R.
  TITLE     Protease-activated receptor 3 is a second thrombin receptor in
            humans
  JOURNAL   Nature 386 (6624), 502-506 (1997)
   PUBMED   9087410
REFERENCE   9  (bases 1 to 3268)
  AUTHORS   Offermanns,S., Laugwitz,K.L., Spicher,K. and Schultz,G.
  TITLE     G proteins of the G12 family are activated via thromboxane A2 and
            thrombin receptors in human platelets
  JOURNAL   Proc Natl Acad Sci U S A 91 (2), 504-508 (1994)
   PUBMED   8290554
REFERENCE   10 (bases 1 to 3268)
  AUTHORS   Shenker,A., Goldsmith,P., Unson,C.G. and Spiegel,A.M.
  TITLE     The G protein coupled to the thromboxane A2 receptor in human
            platelets is a member of the novel Gq family
  JOURNAL   J Biol Chem 266 (14), 9309-9313 (1991)
   PUBMED   1851174
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC026725.7, AK298585.1 and
            AL048674.3.
            
            On Jun 1, 2019 this sequence version replaced NM_001256566.1.
            
            Summary: This gene encodes a member of the protease-activated
            receptor (PAR) family which is a subfamily of the seven
            transmembrane G protein-coupled cell surface receptor family. The
            encoded protein acts as a cofactor in the thrombin-mediated
            cleavage and activation of the protease-activated receptor family
            member PAR4. The encoded protein plays an essential role in
            hemostasis and thrombosis. Alternate splicing results in multiple
            transcript variants that encode different isoforms. [provided by
            RefSeq, Feb 2012].
            
            Transcript Variant: This variant (2) uses an alternate exon in the
            5' UTR and coding region, compared to variant 1. These differences
            cause translation initiation at a downstream AUG and result in an
            isoform (2) with a shorter N-terminus, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK298585.1, DC364035.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AC026725.7         98222-98237         c
            17-1269             AK298585.1         27-1279
            1270-3074           AC026725.7         90510-92314         c
            3075-3268           AL048674.3         1-194
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q13.3"
     gene            1..3268
                     /gene="F2RL2"
                     /gene_synonym="PAR-3; PAR3"
                     /note="coagulation factor II thrombin receptor like 2"
                     /db_xref="GeneID:2151"
                     /db_xref="HGNC:HGNC:3539"
                     /db_xref="MIM:601919"
     exon            1..107
                     /gene="F2RL2"
                     /gene_synonym="PAR-3; PAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            108..3268
                     /gene="F2RL2"
                     /gene_synonym="PAR-3; PAR3"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..1168
                     /gene="F2RL2"
                     /gene_synonym="PAR-3; PAR3"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protease-activated receptor 3; Coagulation factor II
                     receptor-like 2 (protease-actovated receptor 3);
                     proteinase-activated receptor 3; thrombin receptor-like 2"
                     /codon_start=1
                     /product="proteinase-activated receptor 3 isoform 2"
                     /protein_id="NP_001243495.1"
                     /db_xref="CCDS:CCDS58959.1"
                     /db_xref="GeneID:2151"
                     /db_xref="HGNC:HGNC:3539"
                     /db_xref="MIM:601919"
                     /translation="MENDTNNLAKPTLPIKTFRGAPPNSFEEFPFSALEGWTGATITV
                     KIKCPEESASHLHVKNATMGYLTSSLSTKLIPAIYLLVFVVGVPANAVTLWMLFFRTR
                     SICTTVFYTNLAIADFLFCVTLPFKIAYHLNGNNWVFGEVLCRATTVIFYGNMYCSIL
                     LLACISINRYLAIVHPFTYRGLPKHTYALVTCGLVWATVFLYMLPFFILKQEYYLVQP
                     DITTCHDVHNTCESSSPFQLYYFISLAFFGFLIPFVLIIYCYAAIIRTLNAYDHRWLW
                     YVKASLLILVIFTICFAPSNIILIIHHANYYYNNTDGLYFIYLIALCLGSLNSCLDPF
                     LYFLMSKTRNHSTAYLTK"
     regulatory      3246..3251
                     /regulatory_class="polyA_signal_sequence"
                     /gene="F2RL2"
                     /gene_synonym="PAR-3; PAR3"
                     /note="hexamer: AATAAA"
     polyA_site      3268
                     /gene="F2RL2"
                     /gene_synonym="PAR-3; PAR3"
                     /note="major polyA site"
ORIGIN      
        1 ctttccctgc ggaggaccag ggcaagtttc ctgcctgcac ggcacaggag agcaaacttc
       61 tacagacaga ccaaggcttc catttgctgc tgacacatgg aactgaggca tggaaaatga
      121 tacaaacaac ttggcaaagc caaccttacc cattaagacc tttcgtggag ctcccccaaa
      181 ttcttttgaa gagttcccct tttctgcctt ggaaggctgg acaggagcca cgattactgt
      241 aaaaattaag tgccctgaag aaagtgcttc acatctccat gtgaaaaatg ctaccatggg
      301 gtacctgacc agctccttaa gtactaaact gatacctgcc atctacctcc tggtgtttgt
      361 agttggtgtc ccggccaatg ctgtgaccct gtggatgctt ttcttcagga ccagatccat
      421 ctgtaccact gtattctaca ccaacctggc cattgcagat tttctttttt gtgttacatt
      481 gccctttaag atagcttatc atctcaatgg gaacaactgg gtatttggag aggtcctgtg
      541 ccgggccacc acagtcatct tctatggcaa catgtactgc tccattctgc tccttgcctg
      601 catcagcatc aaccgctacc tggccatcgt ccatcctttc acctaccggg gcctgcccaa
      661 gcacacctat gccttggtaa catgtggact ggtgtgggca acagttttct tatatatgct
      721 gccatttttc atactgaagc aggaatatta tcttgttcag ccagacatca ccacctgcca
      781 tgatgttcac aacacttgcg agtcctcatc tcccttccaa ctctattact tcatctcctt
      841 ggcattcttt ggattcttaa ttccatttgt gcttatcatc tactgctatg cagccatcat
      901 ccggacactt aatgcatacg atcatagatg gttgtggtat gttaaggcga gtctcctcat
      961 ccttgtgatt tttaccattt gctttgctcc aagcaatatt attcttatta ttcaccatgc
     1021 taactactac tacaacaaca ctgatggctt atattttata tatctcatag ctttgtgcct
     1081 gggtagtctt aatagttgct tagatccatt cctttatttt ctcatgtcaa aaaccagaaa
     1141 tcactccact gcttacctta caaaatagtg aaatgatctt agagaacaag gacagccatc
     1201 acagagaacg tctgttttca agaacaacat aagcatagtg caaggagctc catttccgag
     1261 ctcctaagaa atatgcttca aaggtcaaac attacaaaag cattagtagt ttgtttgttt
     1321 gtttttgaga ctgagtctca ctttatcacc cagactggcg tgcagtggca ctatcttggc
     1381 tcattgcaac ctctgcctcc caggtcagcc tcccaagtag ctgggattac accaccatgc
     1441 ccagctacta aaaatacttg tatttttagt agagacgggg tttcaccatg ttgaccaggc
     1501 tggtcttgaa ctcctgacct caagtgatct tccggcctca gcctcccaaa gtgctggatt
     1561 acaggcgtga gccactgagc cagccagcat tagtaatttt taaaaacact ttatcagtat
     1621 tttaaaaatg ttaatgcagg agaaaagata tcacaactct atggaaaatg acatttccat
     1681 ttgccttatt gctacttcaa gctctttaaa tcaccatctt ccctatttct gtgagtggta
     1741 ttgccatcct tgacatttga catcattttt tattctttgg tctcttttga ctctcatgct
     1801 ggtggctgcc tcatcaactg attctatctt tgtagggtcc ctcaccaggg tcttttatct
     1861 agtttcacct ttgccttctt ttttcctttt tttaatagag acagtgtctc gcttcgtcac
     1921 ctggctgaag tgcagtagcc tgatcatagc tcacttcagc ctccaactcc tgggctcaag
     1981 caatcctgct gtctcagcct cccaagtaac taagactaca ggcatgcacc accataccca
     2041 actaatttta ttttttattt tttgcagaga tggagtctca ctgttgccca ggctggtctt
     2101 gaactcctgg tctcgagtga tcttcctatc tcagcctccc aaagtgctgg gactacaggc
     2161 atgagccact acgtccaggc cacctctacc ttctaaccat tctctccact gccagcctac
     2221 cctccaccac ttcacaagtc ctgccagatt aatcttcctt agatatcagt taggatacat
     2281 cactaaaaca tttccactaa ctccactgtt tctataataa atcataaatc tttagcctgg
     2341 cattcaaggc taacttgcta atcacacttc ccattaaacc ttgcttatga tataggcacc
     2401 aaccaaacct cttgctctgc tatatctcca ctctgcctca gactgaaaag ctagtcctaa
     2461 cgtctttgcc ttaattctgt tcattttttc aagtccttga aggaccattc ttccctgatt
     2521 ctcaggctag aagtgtcact tttcttatct gtacttccaa agcactttcg tatattttta
     2581 ttatggcatt tatatatagt tcatttatat ttaaatttta attccatgaa caatcaagta
     2641 ccaagtataa tggagaaggt gctcatcctc tgccttcctt gagcttctgg gtgatgccag
     2701 gcccaagtct ttgtggcacc cagctccatg ctttgaatac tatgtggctg aatgaatttt
     2761 taaaatctca aagcagttaa acagcaggaa agcccattaa cttcgtactg aaaaagcaac
     2821 atactgtgat gatacgggat gacatcattt caggttgggc atacaaaaaa gtaaggaagc
     2881 taaactaaga ctatactcac caggccattt agaagtttta aataatgcct ccactatttt
     2941 ttttcttaga catagctttt aatggggaaa tggaattttg ttattaatac ccattatatt
     3001 cctgtaagta aatgactatt tttcccttaa ctcagtgatt agacaggaag gaagacatta
     3061 gtgattagac aggagggaag atattagtga ttagacagca gggaagatat tagtggtaaa
     3121 gagtgaatga tagtagtgaa tataaatggg gctgaggaaa ctttaagcat aaaagattcc
     3181 tgagatgact ttacaagtct gtacgaatct gccttgactg tatatttcat actgcccaac
     3241 aaaacaataa aaaacaattt taaatgca
//
