LOCUS       NM_000504               1536 bp    mRNA    linear   PRI 01-OCT-2024
DEFINITION  Homo sapiens coagulation factor X (F10), transcript variant 1,
            mRNA.
ACCESSION   NM_000504
VERSION     NM_000504.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Stojanovski,B.M. and Di Cera,E.
  TITLE     Conformation of factor Xa in solution revealed by single-molecule
            spectroscopy
  JOURNAL   J Thromb Haemost 22 (10), 2767-2772 (2024)
   PUBMED   39002733
  REMARK    GeneRIF: Conformation of factor Xa in solution revealed by
            single-molecule spectroscopy.
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Fakih,W., Mroueh,A., Gong,D.S., Kikuchi,S., Pieper,M.P., Kindo,M.,
            Mazzucottelli,J.P., Mommerot,A., Kanso,M., Ohlmann,P., Morel,O.,
            Schini-Kerth,V. and Jesel,L.
  TITLE     Activated factor X stimulates atrial endothelial cells and tissues
            to promote remodelling responses through AT1R/NADPH
            oxidases/SGLT1/2
  JOURNAL   Cardiovasc Res 120 (10), 1138-1154 (2024)
   PUBMED   38742661
  REMARK    GeneRIF: Activated factor X stimulates atrial endothelial cells and
            tissues to promote remodelling responses through AT1R/NADPH
            oxidases/SGLT1/2.
REFERENCE   3  (bases 1 to 1536)
  AUTHORS   Pastoret,C., Wahl,C., Castet,S., Nedelec,F., Pontis,A., Bayart,S.,
            Fest,T. and Guillet,B.
  TITLE     Five new F10 variants in hereditary factor x deficiency detected by
            high-throughput sequencing
  JOURNAL   Haemophilia 29 (6), 1565-1572 (2023)
   PUBMED   37842794
  REMARK    GeneRIF: Five new F10 variants in hereditary factor x deficiency
            detected by high-throughput sequencing.
REFERENCE   4  (bases 1 to 1536)
  AUTHORS   Dong,W., Wang,J., Tian,L., Zhang,J., Settles,E.W., Qin,C.,
            Steinken-Kollath,D.R., Itogawa,A.N., Celona,K.R., Yi,J., Bryant,M.,
            Mead,H., Jaramillo,S.A., Lu,H., Li,A., Zumwalt,R.E., Dadwal,S.,
            Feng,P., Yuan,W., Whelan,S.P.J., Keim,P.S., Barker,B.M.,
            Caligiuri,M.A. and Yu,J.
  TITLE     Factor Xa cleaves SARS-CoV-2 spike protein to block viral entry and
            infection
  JOURNAL   Nat Commun 14 (1), 1936 (2023)
   PUBMED   37024459
  REMARK    GeneRIF: Factor Xa cleaves SARS-CoV-2 spike protein to block viral
            entry and infection.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1536)
  AUTHORS   Huang,M.N., Hung,H.L., Stanfield-Oakley,S.A. and High,K.A.
  TITLE     Characterization of the human blood coagulation factor X promoter
  JOURNAL   J Biol Chem 267 (22), 15440-15446 (1992)
   PUBMED   1322399
REFERENCE   6  (bases 1 to 1536)
  AUTHORS   Messier,T.L., Pittman,D.D., Long,G.L., Kaufman,R.J. and Church,W.R.
  TITLE     Cloning and expression in COS-1 cells of a full-length cDNA
            encoding human coagulation factor X
  JOURNAL   Gene 99 (2), 291-294 (1991)
   PUBMED   1902434
REFERENCE   7  (bases 1 to 1536)
  AUTHORS   Hassan,H.J., Leonardi,A., Chelucci,C., Mattia,G., Macioce,G.,
            Guerriero,R., Russo,G., Mannucci,P.M. and Peschle,C.
  TITLE     Blood coagulation factors in human embryonic-fetal development:
            preferential expression of the FVII/tissue factor pathway
  JOURNAL   Blood 76 (6), 1158-1164 (1990)
   PUBMED   1698100
REFERENCE   8  (bases 1 to 1536)
  AUTHORS   Broze,G.J. Jr., Girard,T.J. and Novotny,W.F.
  TITLE     Regulation of coagulation by a multivalent Kunitz-type inhibitor
  JOURNAL   Biochemistry 29 (33), 7539-7546 (1990)
   PUBMED   2271516
  REMARK    Review article
REFERENCE   9  (bases 1 to 1536)
  AUTHORS   Rawala-Sheikh,R., Ahmad,S.S., Ashby,B. and Walsh,P.N.
  TITLE     Kinetics of coagulation factor X activation by platelet-bound
            factor IXa
  JOURNAL   Biochemistry 29 (10), 2606-2611 (1990)
   PUBMED   2110473
REFERENCE   10 (bases 1 to 1536)
  AUTHORS   Leytus,S.P., Chung,D.W., Kisiel,W., Kurachi,K. and Davie,E.W.
  TITLE     Characterization of a cDNA coding for human factor X
  JOURNAL   Proc Natl Acad Sci U S A 81 (12), 3699-3702 (1984)
   PUBMED   6587384
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CB158437.1, BC046125.1 and
            AL137002.19.
            
            On May 17, 2019 this sequence version replaced NM_000504.3.
            
            Summary: This gene encodes the vitamin K-dependent coagulation
            factor X of the blood coagulation cascade. This factor undergoes
            multiple processing steps before its preproprotein is converted to
            a mature two-chain form by the excision of the tripeptide RKR. Two
            chains of the factor are held together by 1 or more disulfide
            bonds; the light chain contains 2 EGF-like domains, while the heavy
            chain contains the catalytic domain which is structurally
            homologous to those of the other hemostatic serine proteases. The
            mature factor is activated by the cleavage of the activation
            peptide by factor IXa (in the intrisic pathway), or by factor VIIa
            (in the extrinsic pathway). The activated factor then converts
            prothrombin to thrombin in the presence of factor Va, Ca+2, and
            phospholipid during blood clotting. Mutations of this gene result
            in factor X deficiency, a hemorrhagic condition of variable
            severity. Alternative splicing results in multiple transcript
            variants encoding different isoforms that may undergo similar
            proteolytic processing to generate mature polypeptides. [provided
            by RefSeq, Aug 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC046125.1, M57285.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000375559.8/ ENSP00000364709.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                CB158437.1         1-19
            20-848              BC046125.1         1-829
            849-849             AL137002.19        72066-72066
            850-1536            BC046125.1         831-1517
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q34"
     gene            1..1536
                     /gene="F10"
                     /gene_synonym="FX; FXA"
                     /note="coagulation factor X"
                     /db_xref="GeneID:2159"
                     /db_xref="HGNC:HGNC:3528"
                     /db_xref="MIM:613872"
     exon            1..127
                     /gene="F10"
                     /gene_synonym="FX; FXA"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..1524
                     /gene="F10"
                     /gene_synonym="FX; FXA"
                     /EC_number="3.4.21.6"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; prothrombinase; factor Xa; Stuart-Prower
                     factor"
                     /codon_start=1
                     /product="coagulation factor X isoform 1 preproprotein"
                     /protein_id="NP_000495.1"
                     /db_xref="CCDS:CCDS9530.1"
                     /db_xref="GeneID:2159"
                     /db_xref="HGNC:HGNC:3528"
                     /db_xref="MIM:613872"
                     /translation="MGRPLHLVLLSASLAGLLLLGESLFIRREQANNILARVTRANSF
                     LEEMKKGHLERECMEETCSYEEAREVFEDSDKTNEFWNKYKDGDQCETSPCQNQGKCK
                     DGLGEYTCTCLEGFEGKNCELFTRKLCSLDNGDCDQFCHEEQNSVVCSCARGYTLADN
                     GKACIPTGPYPCGKQTLERRKRSVAQATSSSGEAPDSITWKPYDAADLDPTENPFDLL
                     DFNQTQPERGDNNLTRIVGGQECKDGECPWQALLINEENEGFCGGTILSEFYILTAAH
                     CLYQAKRFKVRVGDRNTEQEEGGEAVHEVEVVIKHNRFTKETYDFDIAVLRLKTPITF
                     RMNVAPACLPERDWAESTLMTQKTGIVSGFGRTHEKGRQSTRLKMLEVPYVDRNSCKL
                     SSSFIITQNMFCAGYDTKQEDACQGDSGGPHVTRFKDTYFVTGIVSWGEGCARKGKYG
                     IYTKVTAFLKWIDRSMKTRGLPKAKSHAPEVITSSPLK"
     exon            128..288
                     /gene="F10"
                     /gene_synonym="FX; FXA"
                     /inference="alignment:Splign:2.1.0"
     exon            289..313
                     /gene="F10"
                     /gene_synonym="FX; FXA"
                     /inference="alignment:Splign:2.1.0"
     exon            314..427
                     /gene="F10"
                     /gene_synonym="FX; FXA"
                     /inference="alignment:Splign:2.1.0"
     exon            428..559
                     /gene="F10"
                     /gene_synonym="FX; FXA"
                     /inference="alignment:Splign:2.1.0"
     exon            560..804
                     /gene="F10"
                     /gene_synonym="FX; FXA"
                     /inference="alignment:Splign:2.1.0"
     exon            805..922
                     /gene="F10"
                     /gene_synonym="FX; FXA"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1536
                     /gene="F10"
                     /gene_synonym="FX; FXA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1515..1520
                     /regulatory_class="polyA_signal_sequence"
                     /gene="F10"
                     /gene_synonym="FX; FXA"
                     /note="hexamer: ATTAAA"
     polyA_site      1536
                     /gene="F10"
                     /gene_synonym="FX; FXA"
                     /note="major polyA site"
ORIGIN      
        1 gactttgctc cagcagcctg tcccagtgag gacagggaca cagtactcgg ccacaccatg
       61 gggcgcccac tgcacctcgt cctgctcagt gcctccctgg ctggcctcct gctgctcggg
      121 gaaagtctgt tcatccgcag ggagcaggcc aacaacatcc tggcgagggt cacgagggcc
      181 aattcctttc ttgaagagat gaagaaagga cacctcgaaa gagagtgcat ggaagagacc
      241 tgctcatacg aagaggcccg cgaggtcttt gaggacagcg acaagacgaa tgaattctgg
      301 aataaataca aagatggcga ccagtgtgag accagtcctt gccagaacca gggcaaatgt
      361 aaagacggcc tcggggaata cacctgcacc tgtttagaag gattcgaagg caaaaactgt
      421 gaattattca cacggaagct ctgcagcctg gacaacgggg actgtgacca gttctgccac
      481 gaggaacaga actctgtggt gtgctcctgc gcccgcgggt acaccctggc tgacaacggc
      541 aaggcctgca ttcccacagg gccctacccc tgtgggaaac agaccctgga acgcaggaag
      601 aggtcagtgg cccaggccac cagcagcagc ggggaggccc ctgacagcat cacatggaag
      661 ccatatgatg cagccgacct ggaccccacc gagaacccct tcgacctgct tgacttcaac
      721 cagacgcagc ctgagagggg cgacaacaac ctcaccagga tcgtgggagg ccaggaatgc
      781 aaggacgggg agtgtccctg gcaggccctg ctcatcaatg aggaaaacga gggtttctgt
      841 ggtggaacca ttctgagcga gttctacatc ctaacggcag cccactgtct ctaccaagcc
      901 aagagattca aggtgagggt aggggaccgg aacacggagc aggaggaggg cggtgaggcg
      961 gtgcacgagg tggaggtggt catcaagcac aaccggttca caaaggagac ctatgacttc
     1021 gacatcgccg tgctccggct caagaccccc atcaccttcc gcatgaacgt ggcgcctgcc
     1081 tgcctccccg agcgtgactg ggccgagtcc acgctgatga cgcagaagac ggggattgtg
     1141 agcggcttcg ggcgcaccca cgagaagggc cggcagtcca ccaggctcaa gatgctggag
     1201 gtgccctacg tggaccgcaa cagctgcaag ctgtccagca gcttcatcat cacccagaac
     1261 atgttctgtg ccggctacga caccaagcag gaggatgcct gccaggggga cagcgggggc
     1321 ccgcacgtca cccgcttcaa ggacacctac ttcgtgacag gcatcgtcag ctggggagag
     1381 ggctgtgccc gtaaggggaa gtacgggatc tacaccaagg tcaccgcctt cctcaagtgg
     1441 atcgacaggt ccatgaaaac caggggcttg cccaaggcca agagccatgc cccggaggtc
     1501 ataacgtcct ctccattaaa gtgagatccc actcaa
//
