LOCUS       NM_001389628            9986 bp    mRNA    linear   PRI 13-OCT-2024
DEFINITION  Homo sapiens exostosin glycosyltransferase 2 (EXT2), transcript
            variant 4, mRNA.
ACCESSION   NM_001389628
VERSION     NM_001389628.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9986)
  AUTHORS   Wang,Y., Huang,Y., Zhu,H., Guo,Z., Cheng,J., Zhang,C. and Zhong,M.
  TITLE     Exostoisns (EXT1/2) in Head and Neck Cancers: An In Silico Analysis
            and Clinical Correlates
  JOURNAL   Int Dent J 74 (3), 446-453 (2024)
   PUBMED   37989698
  REMARK    GeneRIF: Exostoisns (EXT1/2) in Head and Neck Cancers: An In Silico
            Analysis and Clinical Correlates.
REFERENCE   2  (bases 1 to 9986)
  AUTHORS   Selvan,A.J.A., Kannan,B., Pandi,C., Jayaseelan,V.P. and Arumugam,P.
  TITLE     EXT2: a novel prognostic and predictive biomarker for head and neck
            squamous cell carcinoma
  JOURNAL   Oral Surg Oral Med Oral Pathol Oral Radiol 137 (3), 282-289 (2024)
   PUBMED   38155009
  REMARK    GeneRIF: EXT2: a novel prognostic and predictive biomarker for head
            and neck squamous cell carcinoma.
REFERENCE   3  (bases 1 to 9986)
  AUTHORS   Giambra,M., Di Cristofori,A., Conconi,D., Marzorati,M.,
            Redaelli,S., Zambuto,M., Rocca,A., Roumy,L., Carrabba,G.,
            Lavitrano,M., Roversi,G., Giussani,C. and Bentivegna,A.
  TITLE     Insights into the Peritumoural Brain Zone of Glioblastoma: CDK4 and
            EXT2 May Be Potential Drivers of Malignancy
  JOURNAL   Int J Mol Sci 24 (3), 2835 (2023)
   PUBMED   36769158
  REMARK    GeneRIF: Insights into the Peritumoural Brain Zone of Glioblastoma:
            CDK4 and EXT2 May Be Potential Drivers of Malignancy.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9986)
  AUTHORS   Philippe,C., Porter,D.E., Emerton,M.E., Wells,D.E., Simpson,A.H.
            and Monaco,A.P.
  TITLE     Mutation screening of the EXT1 and EXT2 genes in patients with
            hereditary multiple exostoses
  JOURNAL   Am J Hum Genet 61 (3), 520-528 (1997)
   PUBMED   9326317
REFERENCE   5  (bases 1 to 9986)
  AUTHORS   Clines,G.A., Ashley,J.A., Shah,S. and Lovett,M.
  TITLE     The structure of the human multiple exostoses 2 gene and
            characterization of homologs in mouse and Caenorhabditis elegans
  JOURNAL   Genome Res 7 (4), 359-367 (1997)
   PUBMED   9110175
REFERENCE   6  (bases 1 to 9986)
  AUTHORS   Wuyts,W., Van Hul,W., Wauters,J., Nemtsova,M., Reyniers,E., Van
            Hul,E.V., De Boulle,K., de Vries,B.B., Hendrickx,J., Herrygers,I.,
            Bossuyt,P., Balemans,W., Fransen,E., Vits,L., Coucke,P.,
            Nowak,N.J., Shows,T.B., Mallet,L., van den Ouweland,A.M.,
            McGaughran,J., Halley,D.J. and Willems,P.J.
  TITLE     Positional cloning of a gene involved in hereditary multiple
            exostoses
  JOURNAL   Hum Mol Genet 5 (10), 1547-1557 (1996)
   PUBMED   8894688
REFERENCE   7  (bases 1 to 9986)
  AUTHORS   Stickens,D., Clines,G., Burbee,D., Ramos,P., Thomas,S., Hogue,D.,
            Hecht,J.T., Lovett,M. and Evans,G.A.
  TITLE     The EXT2 multiple exostoses gene defines a family of putative
            tumour suppressor genes
  JOURNAL   Nat Genet 14 (1), 25-32 (1996)
   PUBMED   8782816
REFERENCE   8  (bases 1 to 9986)
  AUTHORS   Wuyts,W., Ramlakhan,S., Van Hul,W., Hecht,J.T., van den
            Ouweland,A.M., Raskind,W.H., Hofstede,F.C., Reyniers,E.,
            Wells,D.E., de Vries,B. et al.
  TITLE     Refinement of the multiple exostoses locus (EXT2) to a 3-cM
            interval on chromosome 11
  JOURNAL   Am J Hum Genet 57 (2), 382-387 (1995)
   PUBMED   7668264
REFERENCE   9  (bases 1 to 9986)
  AUTHORS   Wu,Y.Q., Heutink,P., de Vries,B.B., Sandkuijl,L.A., van den
            Ouweland,A.M., Niermeijer,M.F., Galjaard,H., Reyniers,E.,
            Willems,P.J. and Halley,D.J.
  TITLE     Assignment of a second locus for multiple exostoses to the
            pericentromeric region of chromosome 11
  JOURNAL   Hum Mol Genet 3 (1), 167-171 (1994)
   PUBMED   8162019
REFERENCE   10 (bases 1 to 9986)
  AUTHORS   Wuyts,W., Schmale,G.A., Chansky,H.A. and Raskind,W.H.
  TITLE     Hereditary Multiple Osteochondromas
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301413
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC134775.8, AC068457.5 and
            AC103854.2.
            
            Summary: This gene encodes one of two glycosyltransferases involved
            in the chain elongation step of heparan sulfate biosynthesis.
            Mutations in this gene cause the type II form of multiple
            exostoses. Alternatively spliced transcript variants encoding
            different isoforms have been noted for this gene. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX648142.1, SRR14038194.3099230.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-124               AC134775.8         22356-22479         c
            125-690             AC134775.8         10429-10994         c
            691-780             AC134775.8         9394-9483           c
            781-897             AC134775.8         4376-4492           c
            898-1093            AC068457.5         83640-83835
            1094-1233           AC068457.5         85667-85806
            1234-1327           AC068457.5         88896-88989
            1328-1459           AC068457.5         130462-130593
            1460-1649           AC103854.2         138064-138253       c
            1650-1816           AC103854.2         129123-129289       c
            1817-1960           AC103854.2         103586-103729       c
            1961-2089           AC103854.2         101839-101967       c
            2090-2172           AC103854.2         99707-99789         c
            2173-9986           AC103854.2         84120-91933         c
FEATURES             Location/Qualifiers
     source          1..9986
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p11.2"
     gene            1..9986
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /note="exostosin glycosyltransferase 2"
                     /db_xref="GeneID:2132"
                     /db_xref="HGNC:HGNC:3513"
                     /db_xref="MIM:608210"
     exon            1..124
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /inference="alignment:Splign:2.1.0"
     exon            125..690
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /inference="alignment:Splign:2.1.0"
     CDS             155..2311
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /EC_number="2.4.1.224"
                     /note="isoform 2 is encoded by transcript variant 4;
                     N-acetylglucosaminyl-proteoglycan
                     4-beta-glucuronosyltransferase; exostosin-2; heparan
                     sulfate co-polymerase subunit EXT1; multiple exostoses
                     protein 2; putative tumor suppressor protein EXT2;
                     glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-
                     acetylglucosaminyl-proteoglycan
                     4-alpha-N-acetylglucosaminyltransferase; multiple
                     exostosis 2"
                     /codon_start=1
                     /product="exostosin-2 isoform 2"
                     /protein_id="NP_001376557.1"
                     /db_xref="CCDS:CCDS7908.1"
                     /db_xref="GeneID:2132"
                     /db_xref="HGNC:HGNC:3513"
                     /db_xref="MIM:608210"
                     /translation="MCASVKYNIRGPALIPRMKTKHRIYYITLFSIVLLGLIATGMFQ
                     FWPHSIESSNDWNVEKRSIRDVPVVRLPADSPIPERGDLSCRMHTCFDVYRCGFNPKN
                     KIKVYIYALKKYVDDFGVSVSNTISREYNELLMAISDSDYYTDDINRACLFVPSIDVL
                     NQNTLRIKETAQAMAQLSRWDRGTNHLLFNMLPGGPPDYNTALDVPRDRALLAGGGFS
                     TWTYRQGYDVSIPVYSPLSAEVDLPEKGPGPRQYFLLSSQVGLHPEYREDLEALQVKH
                     GESVLVLDKCTNLSEGVLSVRKRCHKHQVFDYPQVLQEATFCVVLRGARLGQAVLSDV
                     LQAGCVPVVIADSYILPFSEVLDWKRASVVVPEEKMSDVYSILQSIPQRQIEEMQRQA
                     RWFWEAYFQSIKAIALATLQIINDRIYPYAAISYEEWNDPPAVKWGSVSNPLFLPLIP
                     PQSQGFTAIVLTYDRVESLFRVITEVSKVPSLSKLLVVWNNQNKNPPEDSLWPKIRVP
                     LKVVRTAENKLSNRFFPYDEIETEAVLAIDDDIIMLTSDELQFGYEVWREFPDRLVGY
                     PGRLHLWDHEMNKWKYESEWTNEVSMVLTGAAFYHKYFNYLYTYKMPGDIKNWVDAHM
                     NCEDIAMNFLVANVTGKAVIKVTPRKKFKCPECTAIDGLSLDQTHMVERSECINKFAS
                     VFGTMPLKVVEHRADPVLYKDDFPEKLKSFPNIGSL"
     exon            691..780
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /inference="alignment:Splign:2.1.0"
     exon            781..897
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /inference="alignment:Splign:2.1.0"
     exon            898..1093
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1233
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1327
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1459
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1649
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1816
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1817..1960
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1961..2089
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /inference="alignment:Splign:2.1.0"
     exon            2090..2172
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /inference="alignment:Splign:2.1.0"
     exon            2173..9986
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2931..2936
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /note="hexamer: ATTAAA"
     polyA_site      2955
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /note="major polyA site"
     regulatory      3426..3431
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /note="hexamer: ATTAAA"
     polyA_site      3452
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
     regulatory      5194..5199
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /note="hexamer: ATTAAA"
     polyA_site      5213
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
     regulatory      9963..9968
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
                     /note="hexamer: AATAAA"
     polyA_site      9986
                     /gene="EXT2"
                     /gene_synonym="SOTV; SSMS"
ORIGIN      
        1 cccttcctgc tgccaccttc ccgccagcca cagggatctg attcctccca gggggatgtc
       61 ctgcgcctca gggtccggtg gtggcctgcg gcatcccttg cggtgccaga agccgtggga
      121 cgaggagtgt gaggaagagg ctgtctgtgt cattatgtgt gcgtcggtca agtataatat
      181 ccggggtcct gccctcatcc caagaatgaa gaccaagcac cgaatctact atatcaccct
      241 cttctccatt gtcctcctgg gcctcattgc cactggcatg tttcagtttt ggccccattc
      301 tatcgagtcc tcaaatgact ggaatgtaga gaagcgcagc atccgtgatg tgccggttgt
      361 taggctgcca gccgacagtc ccatcccaga gcggggggat ctcagttgca gaatgcacac
      421 gtgttttgat gtctatcgct gtggcttcaa cccaaagaac aaaatcaagg tgtatatcta
      481 tgctctgaaa aagtacgtgg atgactttgg cgtctctgtc agcaacacca tctcccggga
      541 gtataatgaa ctgctcatgg ccatctcaga cagtgactac tacactgatg acatcaaccg
      601 ggcctgtctg tttgttccct ccatcgatgt gcttaaccag aacacactgc gcatcaagga
      661 gacagcacaa gcgatggccc agctctctag gtgggatcga ggtacgaatc acctgttgtt
      721 caacatgttg cctggaggtc ccccagatta taacacagcc ctggatgtcc ccagagacag
      781 ggccctgttg gctggtggcg gcttttctac gtggacttac cggcaaggct acgatgtcag
      841 cattcctgtc tatagtccac tgtcagctga ggtggatctt ccagagaaag gaccaggtcc
      901 acggcaatac ttcctcctgt catctcaggt gggtctccat cctgagtaca gagaggacct
      961 agaagccctc caggtcaaac atggagagtc agtgttagta ctcgataaat gcaccaacct
     1021 ctcagagggt gtcctttctg tccgtaagcg ctgccacaag caccaggtct tcgattaccc
     1081 acaggtgcta caggaggcta ctttctgtgt ggttcttcgt ggagctcggc tgggccaggc
     1141 agtattgagc gatgtgttac aagctggctg tgtcccggtt gtcattgcag actcctatat
     1201 tttgcctttc tctgaagttc ttgactggaa gagagcatct gtggttgtac cagaagaaaa
     1261 gatgtcagat gtgtacagta ttttgcagag catcccccaa agacagattg aagaaatgca
     1321 gagacaggcc cggtggttct gggaagcgta cttccagtca attaaagcca ttgccctggc
     1381 caccctgcag attatcaatg accggatcta tccatatgct gccatctcct atgaagaatg
     1441 gaatgaccct cctgctgtga agtggggcag cgtgagcaat ccactcttcc tcccgctgat
     1501 cccaccacag tctcaagggt tcaccgccat agtcctcacc tacgaccgag tagagagcct
     1561 cttccgggtc atcactgaag tgtccaaggt gcccagtcta tccaaactac ttgtcgtctg
     1621 gaataatcag aataaaaacc ctccagaaga ttctctctgg cccaaaatcc gggttccatt
     1681 aaaagttgtg aggactgctg aaaacaagtt aagtaaccgt ttcttccctt atgatgaaat
     1741 cgagacagaa gctgttctgg ccattgatga tgatatcatt atgctgacct ctgacgagct
     1801 gcaatttggt tatgaggtct ggcgggaatt tcctgaccgg ttggtgggtt acccgggtcg
     1861 tctgcatctc tgggaccatg agatgaataa gtggaagtat gagtctgagt ggacgaatga
     1921 agtgtccatg gtgctcactg gggcagcttt ttatcacaag tattttaatt acctgtatac
     1981 ctacaaaatg cctggggata tcaagaactg ggtagatgct catatgaact gtgaagatat
     2041 tgccatgaac ttcctggtgg ccaacgtcac gggaaaagca gttatcaagg taaccccacg
     2101 aaagaaattc aagtgtcctg agtgcacagc catagatggg ctttcactag accaaacaca
     2161 catggtggag aggtcagagt gcatcaacaa gtttgcttca gtcttcggga ccatgcctct
     2221 caaggtggtg gaacaccgag ctgaccctgt cctgtacaaa gatgactttc ctgagaagct
     2281 gaagagcttc cccaacattg gcagcttatg aaacgtgtca ttggtggagg tctgaatgtg
     2341 aggctgggac agagggagag aacaaggcct cccagcactc tgatgtcaga gtagtaggtt
     2401 aagggtggaa ggttgaccta cttggatctt ggcatgcacc cacctaaccc actttctcaa
     2461 gaacaagaac ctagaatgaa tatccaagca cctcgagcta tgcaacctct gttcttgtat
     2521 ttcttatgat ctctgatggg ttcttctcga aaatgccaag tggaagactt tgtggcatgc
     2581 tccagattta aatccagctg aggctccctt tgttttcagt tccatgtaac aatctggaag
     2641 gaaacttcac ggacaggaag actgctggag aagagaagcg tgttagccca tttgaggtct
     2701 ggggaatcat gtaaagggta cccagacctc acttttagtt atttacatca atgagttctt
     2761 tcagggaacc aaacccagaa ttcggtgcaa aagccaaaca tcttggtggg atttgataaa
     2821 tgccttggga cctggagtgc tgggcttgtg cacaggaaga gcaccagccg ctgagtcagg
     2881 atcctgtcag ttccatgagc tattcctctt tggtttggct ttttgatatg attaaaatta
     2941 ttttttattc ctttttctac tgtgtcttaa acaccaattc ctgatagtcc aaggaaccac
     3001 ctttctccct tgatatattt aactccgtct ttggcctgac aacagtcttc tgcccatgtc
     3061 tgggaacaca cgccaggagg aatgtctgat accctctgca tcaagcgtaa gaaggtccca
     3121 aatcataacc attttaagaa cagatgactc agaaacctcc agaggaatct gtttgcttcc
     3181 tgattagatc cagtcaatgt tttaaaggta ttgtcagaga aaaacagagg gtctgtacta
     3241 gccatgcaag gagtcgctct agctggtacc cgtaaaagtt gtgggaattg tgacccccat
     3301 cccaagggga tgccaaaatt tctctcattc ttttggtata aacttaacat tagccaggga
     3361 ggttctggct aacgttaaat gctgctatac aactgctttg caacagttgc tggtatattt
     3421 aaatcattaa atttcagcat ttactaatac tgcacatgtg tgaattatac ctctttaagc
     3481 ccagttgatg aacaaatcta ccctggcgaa tgttaaatgt tatggattcg aaacagattt
     3541 atctggctct gatattaaga ttagccacag tttgggcttt agccacaaca tatgtcccca
     3601 aaacacaaaa tacataacaa tttgcttaga atatggatat aattacagaa acctagttgt
     3661 tttgccagtc agcgtcagtc atacaccatc cattgttctg ttgagtgaca tgaaggtggg
     3721 cagttattat gctgtagttt catcaagagt cacttcagat gtgttcccca gactttgaga
     3781 ggtcaaagaa agtttcagaa gtagcaaagt tggaagtgga aatcataaga gttttgtctt
     3841 ctttcatttg aataagtgat cgaatcatgg actattattg ccaaaggggg caagcgatca
     3901 tctcatccag tttgttcatt ttgcaaatgc agaaactgaa gctgcagatg ttcattttgc
     3961 aaatgcagaa actgaagtga tttttttcag tttctgacag cagcaaacac aggtgcttac
     4021 caaatacttt tgatttgggt aatgtgtact ggtcagtagg agacatggga aagcattttg
     4081 gtctcatgta ttcataatat tggggacgat atggtagaaa gccaggaaaa atatttctgt
     4141 tgtcacccag gtaccagagg ctgacaagca cagtgaggac ctctaatctt tctaggggtt
     4201 ttctagtttg ttttccaaga tgtggggcag gtatagggga gggtcttaca ttgaaatctc
     4261 tgctgaaagc tatggactgt gctggcattg tgtatgtgtc atattgccct ggtttcttca
     4321 aatttctgcc cttttgctca tcatcgaatg cactacctta gtggctggtt tctaatgcag
     4381 cttctctccc aaagggtaca tttttaaatt tttatttttt aaaatcttga gagtcctttg
     4441 atttgggaag aggaactctt tcatctgagt tcattgtgag ccctagagag ctgtgtgact
     4501 ttatcatcgt cataggagtc attggacttt aaaggcatta caactccatt taaagtacct
     4561 cattactccc aggaatattg ggtgttagat cttgcaacac agcataaaac aatttatgtt
     4621 aaagaaggag cattatatcc taggcacttg aaacaacctc aaaggtgatg ttaccctatg
     4681 aacagatagg tggtacagtc accgtagaaa ttcaagacag ggatatagca ggtgatggcg
     4741 attcatggat tcagagtggg ctagggactc cactttattg ttcgtgtatt tctgagctgc
     4801 taaccagcaa acctcaccca taagcttagg tcaggatcat ctgtcctcgg gatgccatag
     4861 agcctggttt cagaagcaga acaatcatgc taattaaaat gtcgtgttct ggcctacagc
     4921 agacaacagc tggttttaga ttaagaaatc tttttaaaga aacacatctt actggcctat
     4981 agagaagacg aacttgaact ggccctggcc caagtgcaca ctgagccact ggctttgccc
     5041 ccaggggcct aactgggatg ggcctgcttg gaatgccagg gctggaaata cttttttatt
     5101 ttgctgatta acattacata gttgttaaaa tgtttatctc atgtatttta ccaaccctca
     5161 cacatttagg tataaacctc cttttctggg agaattaaag agaaaaacag caactttagt
     5221 tgtcagaatc ttgtgttctt tcctgccagt gatgctctgc tgtatccttt tttttttttt
     5281 tttttttgag acagagtctt gctcttgtca cccaggctgg agtgcaacaa tagcatggtc
     5341 tcggctcact acatcctccg cctcccaggt tcaagtgatt ctcctgcctc agcctcctga
     5401 gtagctggga ttacaggcgc ttgccaccac gcctggctaa tttgtttgta attttagtag
     5461 aggcagggtt tcaccacgtt agccaggctg gtctcgaact cctgacctca ggtgatctgc
     5521 acgccttgac ctcccaaagt gctgggatta cgggcgtgag tccccacgct cagccactgt
     5581 atcctttact cagtgtcatc aagttcaaag ctctgaccac cctggtttct ctgccaaagc
     5641 ctcatctact tccagctggg ctttgaactt gtggttttta agtgctggtt tctctgctgc
     5701 ctttggaaaa tagtgaccat catgctggtc tgtgctgacg cagactggga gctggaagag
     5761 acaagctccc tctttgctct cattggccaa agatgttgct aataaccctg tgatcctagc
     5821 ttccttctag gatgatagcc tctgacaggc accatgccag gaggaaacta gagcgtcttt
     5881 atcactgaaa gatagcacgt ttctagccac aggttctgaa gttgatttga ggttaaaatc
     5941 caagaacctt tgatagtttt cagccaagat agtaatcaga aagtagatga agcagagact
     6001 gggtttggga agtggttggg ttctgggaag gctcttccta gctctgcagt tagatcctgt
     6061 gaggacccag ctgatagagt aaaggctttg ggtgttggag acttcccatc agccacccat
     6121 catgagcctg gtgtgtttcc tttgctttgt agctcctttg ggggtatgct gctttttcca
     6181 tttctcttgg ctcgggtact cttccattgc agcaagagaa gccacagtgg gctgttcccc
     6241 aagcagccct ggcacagaga ggcagagtag agaatgctaa actggcccca tgtctcctcc
     6301 agccacttcc tgaggagcag ttctggcagg agtgttaacc atattggcag cagcttccat
     6361 ccagagagac tttgaaagca acttgagctt tccactgcag ggcctggagg catatgaaac
     6421 ctgtggggcc tgcccctact cacctcaccc ctgagctctg tccaagccct ggacctcctg
     6481 tcctcggcct gtgtttggta ctcacccact ttctacttct gagtagaatc aagggcagca
     6541 tttggtccca ttataccagc catgcatcat ctgtgaacag gccttattga gcttatcttg
     6601 gcagaggaaa gtcaggcagg caagacccac tctaaatttt aatgtttccc cctcatttta
     6661 gggtgggagc tgtgagttca tctgaagact gggtgagggc attgtaagct caagccacag
     6721 cctcttttcc acctccatta aatggactgt tagggatgcc cagcttcccc aatttcctgg
     6781 gagggtgtgt gggtctgtac aggcaacctt gtgtcattct ttcaatttcc ggctcaccat
     6841 tgctggcaac agtgggaaga tgaccagaga aagtttggcc ccctctccca agcttccagg
     6901 cctctttcct tttcaagaaa tagttgagtc accagcatct gtatctggca ttttgtttct
     6961 tcacatttgc aaccatttca gggtagagtt tttttgtttg tttgtttgtt tgtttttgtt
     7021 ttttttgttt tttctttgga aacagagtct cactctgtcc cataggctgg agtgtagtgc
     7081 tgtgatcatg gctcgctgca gcctcaaact cctggactca agcagtcttc ccatctcagc
     7141 cttccaagta gctgagacta aaagcgtgca ccaccacacc tggctgatct tttaattttt
     7201 ttggagagac agggcctcac tatgttgcct aggctggtct ctaactcctg ggatcaagtg
     7261 atcctcctgc ctcagcgtcc caaagtgttg ggattacagg tgtgaaccac catgcctggc
     7321 ccaggatagc actttttcct ttcttctgct ctcagttggt tggaccactg gttgcatctc
     7381 ttcccactct ccctgaaagg cacagaacta tcagacagaa gacaaggtag ttctcaagaa
     7441 gtaccaggca gctgtgtctg ctagaggctt cctcagcaaa gccccatctt gtggacaacc
     7501 tctcaggaat tgcaggtggt gtgggagtgg ctgacagagg agggatccat atgtcttaga
     7561 ccaaagccac cagtcaggcc ttgccaccat atagaacctt ccctttagaa tatatttatt
     7621 tggaatttta cttatagctg caaatatatc attggctact atacccaagc atcaactcaa
     7681 ggaacatttg gttttctggt gagcacagct gaagaccaac caactagtta ctgatccaga
     7741 tgagggaaga gaacatttat tcagtcattc tgttatgcta actattcatt ttctcatgaa
     7801 aatgtagtct ttggctgggc atggtggctc atgcctgtaa tcccagcatt ttgggaggcc
     7861 aaagcgggca gatcatgagg tcacgagatc gagaccctcc tggctaacac ggtgaaaccc
     7921 catctctact aaaaatacaa agaattagcc gggcatagtg gcaggcacct gtagccccag
     7981 ctactcggga ggctgaggca ggagaatggc gtgaacctga gaggtggagc ttgcagtgag
     8041 ctgagattgt gccactacac tccagcctgg gcgacagagc aagactccgt ctcaaaagag
     8101 aaaatgttgt ctttgccagg cagtgtggta ggcccaagag atatagcaat tagagttgac
     8161 taagacagtg tccctatcct gttcttgagc tccagttcta gtgggagttc ttgagcttgg
     8221 taagatgatg tgagtccatc agtggcccag gactctaagc atgtatgttt tagtcccagc
     8281 tgtaaatgag gattccatta accccatctt ctccaatgta tggaggatgc aagaagcttt
     8341 aaaatgtcct tttctcacag tgacttccct tgaccatgcc ttagtttcct cagccagaaa
     8401 ttggagacag caagagcact tatgcctgag ttggtgagat ggaatgatgt atcatatctc
     8461 ttcccctttt attaaccaga tcgtttacca gattgtacct ggtgatgctg gcagccagcc
     8521 ctcttccggc ccctatttct gcttaccgtg ttaccagaga gcctgggggt ctggatccta
     8581 tctggccccg tcagggtgga ttaccaaatg agcagttctc ttgccccagt ccctttcctg
     8641 tgctataaat aagccccatg tttattttct tatgttattg aaatgagcac ttgtgatttg
     8701 ggcctctttt gaggagtcca gagagcgtcc atccggtgcc tggtgagggc cctgcatggc
     8761 tggctgctgt ctgaagctat ttggagtcct ctccctgtgt tttctatgtg gcttaatttc
     8821 aatagaaagg gttatatgca accctgtatc tgctgatttt caggtttcaa ctttctgcca
     8881 gcgtcactgc ctgcttagaa gtaaagttat gtttctcatt aaggggataa cagccacaat
     8941 tgaggtaatt aacgaaaatt gtacattggt ggcagcacct cctataggat ttccaatagt
     9001 ctttctctag tagatcattg ggggctcacc ttgatctcct ctcttctgtc taccctgcac
     9061 caaaatacct tgtcctgttt tctggatata gttccaataa tttttttcct aacagccttt
     9121 ttgtcaccag ttggtttgat atcttacaac ttggccaaat gagggttcca ttaactccat
     9181 cttgtctaat gcatggagaa ttcaaggatt ttttttttct cttttcatag caccttccag
     9241 ttgccagttg taccctggcc cttctttgga agtcataatg atgaatatcc attaataaga
     9301 gattgatgct ctttcaactc tcatgtcatc tataccatct cagtggagag gatgactttg
     9361 gatgaggttg gaatagaaag gaaacatttg gaagtccact gcagtgtatt atatgctgtg
     9421 tggaagtctg ggggttagga aatacctgga gggagaactt cctaagaaat gatttttggt
     9481 tcttttaggc cttaacagca caataaaagt atcccatgag accattatga gcaggacacg
     9541 acattgtttc acaccttggg ctgtgactat ttacttctcg gtacagatta ctctggttaa
     9601 atcactcagt aaagaaatct tttcatgctc acaatctgaa cctgaaggct attactgaag
     9661 agaattgcat ctgacaacaa aatttaattt acttccagag aaaggaccag aagaaagtaa
     9721 attttcattt atgtttttaa gtctattgtc ttaaaaagat tcttttccct taaaaaataa
     9781 aaaaacctga tgtgatgggt tccttcagtc aacaaatact tattgagcag ttattgtgtg
     9841 ccagatactg ttcttggtgt gaggatatgg cactgaacaa aacaatgtac ctactttcgt
     9901 caagcttaca ttctaatgag gaagataacc aaaacaagtg actgaatata atttcaaatg
     9961 tcaataaatg ctgtgaagaa aataaa
//
