LOCUS       NM_000127               8243 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens exostosin glycosyltransferase 1 (EXT1), mRNA.
ACCESSION   NM_000127
VERSION     NM_000127.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Bartolotti,I., Sobul,K., Corsini,S., Scognamiglio,D., Moroni,A.,
            Gnoli,M., Sangiorgi,L. and Pedrini,E.
  TITLE     A Novel Pathogenic Large Duplication in EXT1 Identified in a Family
            with Multiple Osteochondromas
  JOURNAL   Genes (Basel) 15 (9), 1169 (2024)
   PUBMED   39336760
  REMARK    GeneRIF: A Novel Pathogenic Large Duplication in EXT1 Identified in
            a Family with Multiple Osteochondromas.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8243)
  AUTHORS   Solaimuthu,B., Khatib,A., Tanna,M., Karmi,A., Hayashi,A., Abu
            Rmaileh,A., Lichtenstein,M., Takoe,S., Jolly,M.K. and Shaul,Y.D.
  TITLE     The exostosin glycosyltransferase 1/STAT3 axis is a driver of
            breast cancer aggressiveness
  JOURNAL   Proc Natl Acad Sci U S A 121 (3), e2316733121 (2024)
   PUBMED   38215181
  REMARK    GeneRIF: The exostosin glycosyltransferase 1/STAT3 axis is a driver
            of breast cancer aggressiveness.
REFERENCE   3  (bases 1 to 8243)
  AUTHORS   Ye,T., Jiang,M., Zeng,X., Zong,D., Du,Y., Li,X., Huang,B. and
            Tang,X.
  TITLE     Clinical significance of exostosin 1 in confirmed and suspected
            lupus membranous nephropathy
  JOURNAL   Lupus Sci Med 10 (2), e001051 (2023)
   PUBMED   38154829
  REMARK    GeneRIF: Clinical significance of exostosin 1 in confirmed and
            suspected lupus membranous nephropathy.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8243)
  AUTHORS   Ludecke,H.J., Ahn,J., Lin,X., Hill,A., Wagner,M.J., Schomburg,L.,
            Horsthemke,B. and Wells,D.E.
  TITLE     Genomic organization and promoter structure of the human EXT1 gene
  JOURNAL   Genomics 40 (2), 351-354 (1997)
   PUBMED   9119404
REFERENCE   5  (bases 1 to 8243)
  AUTHORS   Ahn,J., Ludecke,H.J., Lindow,S., Horton,W.A., Lee,B., Wagner,M.J.,
            Horsthemke,B. and Wells,D.E.
  TITLE     Cloning of the putative tumour suppressor gene for hereditary
            multiple exostoses (EXT1)
  JOURNAL   Nat Genet 11 (2), 137-143 (1995)
   PUBMED   7550340
REFERENCE   6  (bases 1 to 8243)
  AUTHORS   Cook,A., Raskind,W., Blanton,S.H., Pauli,R.M., Gregg,R.G.,
            Francomano,C.A., Puffenberger,E., Conrad,E.U., Schmale,G.,
            Schellenberg,G. et al.
  TITLE     Genetic heterogeneity in families with hereditary multiple
            exostoses
  JOURNAL   Am J Hum Genet 53 (1), 71-79 (1993)
   PUBMED   8317501
REFERENCE   7  (bases 1 to 8243)
  AUTHORS   Tuysuz,B., Gunes,N. and Alkaya,D.U.
  TITLE     Trichorhinophalangeal Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   28426188
REFERENCE   8  (bases 1 to 8243)
  AUTHORS   Wuyts,W., Schmale,G.A., Chansky,H.A. and Raskind,W.H.
  TITLE     Hereditary Multiple Osteochondromas
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301413
REFERENCE   9  (bases 1 to 8243)
  AUTHORS   Ogle,R.F., Dalzell,P., Turner,G., Wass,D. and Yip,M.Y.
  TITLE     Multiple exostoses in a patient with t(8;11)(q24.11;p15.5)
  JOURNAL   J Med Genet 28 (12), 881-883 (1991)
   PUBMED   1757967
REFERENCE   10 (bases 1 to 8243)
  AUTHORS   Parrish,J.E., Wagner,M.J., Hecht,J.T., Scott,C.I. Jr. and
            Wells,D.E.
  TITLE     Molecular analysis of overlapping chromosomal deletions in patients
            with Langer-Giedion syndrome
  JOURNAL   Genomics 11 (1), 54-61 (1991)
   PUBMED   1684953
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP003476.2, BC001174.1 and
            AC113188.6.
            
            On Nov 21, 2019 this sequence version replaced NM_000127.2.
            
            Summary: This gene encodes an endoplasmic reticulum-resident type
            II transmembrane glycosyltransferase involved in the chain
            elongation step of heparan sulfate biosynthesis. Mutations in this
            gene cause the type I form of multiple exostoses. [provided by
            RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: S79639.1, BC001174.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378204.7/ ENSP00000367446.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-614               AP003476.2         78725-79338         c
            615-3370            BC001174.1         1-2756
            3371-8243           AC113188.6         57853-62725         c
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.11"
     gene            1..8243
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
                     /note="exostosin glycosyltransferase 1"
                     /db_xref="GeneID:2131"
                     /db_xref="HGNC:HGNC:3512"
                     /db_xref="MIM:608177"
     exon            1..1742
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
                     /inference="alignment:Splign:2.1.0"
     CDS             781..3021
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
                     /EC_number="2.4.1.225"
                     /note="exostoses (multiple) 1;
                     Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-
                     acetylglucosaminyltransferase;
                     N-acetylglucosaminyl-proteoglycan
                     4-beta-glucuronosyltransferase; Langer-Giedion syndrome
                     chromosome region; multiple exostoses protein 1; putative
                     tumor suppressor protein EXT1;
                     glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-
                     acetylglucosaminyl-proteoglycan
                     4-alpha-N-acetylglucosaminyltransferase; heparan sulfate
                     co-polymerase subunit EXT1"
                     /codon_start=1
                     /product="exostosin-1"
                     /protein_id="NP_000118.2"
                     /db_xref="CCDS:CCDS6324.1"
                     /db_xref="GeneID:2131"
                     /db_xref="HGNC:HGNC:3512"
                     /db_xref="MIM:608177"
                     /translation="MQAKKRYFILLSAGSCLALLFYFGGLQFRASRSHSRREEHSGRN
                     GLHHPSPDHFWPRFPDALRPFVPWDQLENEDSSVHISPRQKRDANSSIYKGKKCRMES
                     CFDFTLCKKNGFKVYVYPQQKGEKIAESYQNILAAIEGSRFYTSDPSQACLFVLSLDT
                     LDRDQLSPQYVHNLRSKVQSLHLWNNGRNHLIFNLYSGTWPDYTEDVGFDIGQAMLAK
                     ASISTENFRPNFDVSIPLFSKDHPRTGGERGFLKFNTIPPLRKYMLVFKGKRYLTGIG
                     SDTRNALYHVHNGEDVVLLTTCKHGKDWQKHKDSRCDRDNTEYEKYDYREMLHNATFC
                     LVPRGRRLGSFRFLEALQAACVPVMLSNGWELPFSEVINWNQAAVIGDERLLLQIPST
                     IRSIHQDKILALRQQTQFLWEAYFSSVEKIVLTTLEIIQDRIFKHISRNSLIWNKHPG
                     GLFVLPQYSSYLGDFPYYYANLGLKPPSKFTAVIHAVTPLVSQSQPVLKLLVAAAKSQ
                     YCAQIIVLWNCDKPLPAKHRWPATAVPVVVIEGESKVMSSRFLPYDNIITDAVLSLDE
                     DTVLSTTEVDFAFTVWQSFPERIVGYPARSHFWDNSKERWGYTSKWTNDYSMVLTGAA
                     IYHKYYHYLYSHYLPASLKNMVDQLANCEDILMNFLVSAVTKLPPIKVTQKKQYKETM
                     MGQTSRASRWADPDHFAQRQSCMNTFASWFGYMPLIHSQMRLDPVLFKDQVSILRKKY
                     RDIERL"
     exon            1743..1836
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..1944
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
                     /inference="alignment:Splign:2.1.0"
     exon            1945..2064
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
                     /inference="alignment:Splign:2.1.0"
     exon            2065..2197
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
                     /inference="alignment:Splign:2.1.0"
     exon            2198..2316
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
                     /inference="alignment:Splign:2.1.0"
     exon            2317..2412
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
                     /inference="alignment:Splign:2.1.0"
     exon            2413..2502
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
                     /inference="alignment:Splign:2.1.0"
     exon            2503..2663
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
                     /inference="alignment:Splign:2.1.0"
     exon            2664..2835
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
                     /inference="alignment:Splign:2.1.0"
     exon            2836..8243
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3339..3344
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
                     /note="hexamer: ATTAAA"
     polyA_site      3370
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
                     /note="major polyA site"
     polyA_site      5166
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
     regulatory      8223..8228
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
                     /note="hexamer: AATAAA"
     polyA_site      8243
                     /gene="EXT1"
                     /gene_synonym="EXT; LGCR; LGS; TRPS2; TTV"
ORIGIN      
        1 aacattcggc gaccgaacgc ggcggtcggc agcgttcgcg cgggggcctg cgaagcgctg
       61 ctcggggccg gcactgcccg cggggaggac gcgccgccgc cgccacccag cgccgccgcc
      121 gccgccgcct ccagccgggc cgccgcgcgt cccgggggcc ggccccgcga gcgcaggagt
      181 aaacaccgcc ggagtcttgg agccgctgca gaagggaata aagagagatg cagggatttg
      241 tgaggttacg gcgccccagc tgcaagatgc actagccggc tgaacccggg atcggctgac
      301 ttgttggaac cggagtgctc tgcacggaga gtggtggatg agttgaagtt gccttcccgg
      361 ggctcatttt ccacgctgcc gagaggaatc cgagaggcaa ggcaatcact tcgtcttgcc
      421 attgattggg tatcgggagc tttttttttc tcccctctct ctttcttttc ctccgtcttg
      481 ttgcatgcaa gaaaattaca gtccgctgct cgcccgccct gggtgcgaga tattcagccc
      541 cgctctctcc cgtgcattgt gcaacccaaa gatgaaagac cgaaggggag aaagttaaag
      601 aaatcgccca catgcgctgg atcagtccac ggcttgggga aaggcatcca gagaaggtgg
      661 gagcggagag tttgaagtct ttacaggcgg gaagatggcg gactggagct gaaagtgttg
      721 attgggaaac ttgggtgatt cttgtgttta tttacaatcc tcttgaccca ggcaggacac
      781 atgcaggcca aaaaacgcta tttcatcctg ctctcagctg gctcttgtct cgcccttttg
      841 ttttatttcg gaggcttgca gtttagggca tcgaggagcc acagccggag agaagaacac
      901 agcggtagga atggcttgca ccaccccagt ccggatcatt tctggccccg cttcccggac
      961 gctctgcgcc ccttcgttcc ttgggatcaa ttggaaaacg aggattccag cgtgcacatt
     1021 tccccccggc agaagcgaga tgccaactcc agcatctaca aaggcaagaa gtgccgcatg
     1081 gagtcctgct tcgatttcac cctttgcaag aaaaacggct tcaaagtcta cgtataccca
     1141 cagcaaaaag gggagaaaat cgccgaaagt taccaaaaca ttctagcggc catcgagggc
     1201 tccaggttct acacctcgga ccccagccag gcgtgcctct ttgtcctgag tctggatact
     1261 ttagacagag accagttgtc acctcagtat gtgcacaatt tgagatccaa agtgcagagt
     1321 ctccacttgt ggaacaatgg taggaatcat ttaattttta atttatattc cggcacttgg
     1381 cctgactaca ccgaggacgt ggggtttgac atcggccagg cgatgctggc caaagccagc
     1441 atcagtactg aaaacttccg acccaacttt gatgtttcta ttcccctctt ttctaaggat
     1501 catcccagga caggagggga gagggggttt ttgaagttca acaccatccc tcctctcagg
     1561 aagtacatgc tggtattcaa ggggaagagg tacctgacag ggataggatc agacaccagg
     1621 aatgccttat atcacgtcca taacggggag gacgttgtgc tcctcaccac ctgcaagcat
     1681 ggcaaagact ggcaaaagca caaggattct cgctgtgaca gagacaacac cgagtatgag
     1741 aagtatgatt atcgggaaat gctgcacaat gccactttct gtctggttcc tcgtggtcgc
     1801 aggcttgggt ccttcagatt cctggaggct ttgcaggctg cctgcgtccc tgtgatgctc
     1861 agcaatggat gggagttgcc attctctgaa gtgattaatt ggaaccaagc tgccgtcata
     1921 ggcgatgaga gattgttatt acagattcct tctacaatca ggtctattca tcaggataaa
     1981 atcctagcac ttagacagca gacacaattc ttgtgggagg cttatttttc ttcagttgag
     2041 aagattgtat taactacact agagattatt caggacagaa tattcaagca catatcacgt
     2101 aacagtttaa tatggaacaa acatcctgga ggattgttcg tactaccaca gtattcatct
     2161 tatctgggag attttcctta ctactatgct aatttaggtt taaagccccc ctccaaattc
     2221 actgcagtca tccatgcggt gacccccctg gtctctcagt cccagccagt gttgaagctt
     2281 ctcgtggctg cagccaagtc ccagtactgt gcccagatca tagttctatg gaattgtgac
     2341 aagcccctac cagccaaaca ccgctggcct gccactgctg tgcctgtcgt cgtcattgaa
     2401 ggagagagca aggttatgag cagccgtttt ctgccctacg acaacatcat cacagacgcc
     2461 gtgctcagcc ttgacgagga cacggtgctt tcaacaacag aggtggattt cgccttcaca
     2521 gtgtggcaga gcttccctga gaggattgtg gggtaccccg cgcgcagcca cttctgggat
     2581 aactctaagg agcggtgggg atacacatca aagtggacga acgactactc catggtgttg
     2641 acaggagctg ctatttacca caaatattat cactacctat actcccatta cctgccagcc
     2701 agcctgaaga acatggtgga ccaattggcc aattgtgagg acattctcat gaacttcctg
     2761 gtgtctgctg tgacaaaatt gcctccaatc aaagtgaccc agaagaagca gtataaggag
     2821 acaatgatgg gacagacttc tcgggcttcc cgttgggctg accctgacca ctttgcccag
     2881 cgacagagct gcatgaatac gtttgccagc tggtttggct acatgccgct gatccactct
     2941 cagatgaggc tcgaccccgt cctctttaaa gaccaggtct ctattttgag gaagaaatac
     3001 cgagacattg agcgactttg aggaatccgg ctgagtgggg gaggggaagc aagaagggat
     3061 gggggtcaag ctgctctctc ttcccagtgc agatccactc atcagcagag ccagattgtg
     3121 ccaactatcc aaaaacttag atgagcagaa tgacaaaaaa aaaaaggcca atgagaactc
     3181 aactcctggc tcctgggact gcaccagact gctccaaact cacctcactg gcttctgtgt
     3241 cccaagacta ggttgtgtac agtttaatta tggaacatta aataattatt tttgaaatga
     3301 ttgctatgca ggtttaaact tttttaatga tcaaaactat taaaaaccag agttctttgt
     3361 ttaatcaaaa ttgtgttggt tgtgaatatt tcaaagctgc tattcctttt cccacagaca
     3421 tcattgtcat ggccatgtag ggtgccctgc agtttcaaaa gctcaaactt cgtggaaaac
     3481 acaataagtc actctaccca ttatcaagaa ataactgagc ataagttgta acttcattat
     3541 tcaactttgc cagtgcaaat tgttttccac ttcgaatctt caaatccact tgaactttta
     3601 tctctaaaat gtcgctgcat gaaagaaagt attacgactt ccaggtaggc agttctaact
     3661 gaaatctcta tgtttgagat agatatatat gataatcgtt tttcattggg ggggtggggg
     3721 gaattagtac caagaaaaca ctagtataat taagaaatgt tcagtttgca caaagaacta
     3781 tccagataac ccaccagcat gttagtgaga tggaaataca gacccacaac agtaacccaa
     3841 tacttgcagg ggttgggggc acggttatag attcaaccat tgacctaagt ctgcgtagca
     3901 ctgggaagag gctttggttt agaagccaag gaataatgag tatattgggg agaacagatt
     3961 atttacaaga tgaactcttt aatgtttgtg agaatctcaa gtttcagagt ttctcttttg
     4021 agaaagaaaa aggggtaata aggtagaaat tcacaccaat gaacaagagg attgctgcaa
     4081 agtaactgag gagatgtctc gccattggga ccctaatgcc atttttggtc aaacattgtt
     4141 ttgagcaaga atctggcaaa caaaataatc aacaacaaat gtgaatatag tttcatttac
     4201 ttttaatttt taaatctgtg gaaaagttta gttgtgcttc ttgttaaaaa gaacatttct
     4261 atccctgaaa atgctatctt gggcttatga ttattgttaa actccaagta taaactgaaa
     4321 aaaaaaacat atccctaact ctgttatgaa aaatggagac ttctgatatt aaatgctttc
     4381 ttctacttgg aagaggccag agaaaacagg gaagagaaag acattattga gtttgaccat
     4441 gtattatgct gaataaacaa taagcacttt agagtccctc cctcaaactc tcatacattt
     4501 catatttctt ttccatttat tttcagtttt gttttagaag aaaagttcat cagagaattt
     4561 tgttctgata atttcaagtg gccatcttag gtcagtggaa accgtaagtc cattggactt
     4621 tacctcatcc tttctttgta gacatctggg agaggaagag gagcttgtaa tgatagcacg
     4681 gggatggtgc tgtaaacagg agtgaaagtg tttgtggaag tccagagaag tgactcaaca
     4741 ggctacctag ctgcagaaca aggagtagag cccacatatc cagatggtgt tttgagaggt
     4801 gcgtagacag tgaaattcaa ttaaagagag gattttctcc tcagcctcat gactcagaac
     4861 agctccctaa atacctctct catctaattg gacccatcca tagtccattc tcaaacatgt
     4921 gacattttcc cctaagtaga tgtgattatc tcttaggcat ttgttgaaaa ataattctta
     4981 ggtccatggt gcttgcactt gtgtcttttc tataaaatgt ttggtcttac aggtttcatg
     5041 tcatttaagc tgcacttctc agcaagttaa aagattagca gttaatcttt attcatttgg
     5101 cctttgtgaa tttgtattta aattattttt ttcaggattg gcaaattatt cttgtttctc
     5161 ctttaccaaa aaataaatgc gatattttgt tcaatgaccc caaaaccaac ttgaaactta
     5221 ggtggtcata ttggcttgca aagcaatgtc cctaattgta ccagtcagtc acacaagtgg
     5281 attcaaggac ctgctttgcc agattgacct gtcaccaagc tcacaacaca tatcctccac
     5341 aaacaacatg tgttatgtga agaaaaatgg taattataaa taagaacagg atataacaca
     5401 cctttcatcc actttaaatc tccacagttt cattttatgt cattctctga gcaaatctct
     5461 ttgggatgtg agctagcgtg ttcttctccc atttggaata taaggctggg aatagaacaa
     5521 tgcttaacaa atcagtgaag ctcgacagta atatgtaatt ttaattcagt taggaaagag
     5581 ttgtattgca ttgcaacaag ttgacaaatc atagccatct ctgtagggtg tcaggaatta
     5641 tctcctggtc aactttaatg ataactaggg gtccctaagt gggctaacat gtgctgcatt
     5701 ggagagaagc caagggctga gagtacagtg ccaaccacgt aatgaattgc ttgcagaaat
     5761 tccaaagagg actcagccac ttatggattt ccacacagca tcttttccca gctccacatt
     5821 aagacacagg atcttaaaag taatttttta aaagctggct gtgtatttat ttatatgaaa
     5881 gtgttataaa tatcaaagct tacaaataca ttaatacata tcacctttgt tgaagctagc
     5941 aaaatggctc aaaattgaga ctgtagagaa aaatccatga aataatcacg atagtcatac
     6001 cacaaaagat agcatagcta ggtgggccta cctggtcgta tcaacacact ttcagataga
     6061 tgcttcaaaa aaaaagggaa agcttgttac tggaatctta tgtgcattac agtttaattt
     6121 cttcttgtga caaatggtgc tcaaagaagg atcaatccgg ttgcatcctt caatttccct
     6181 tttagaatga cttttggttt tcattacagt aggctatgtt agcctttatt ttggtggttc
     6241 tcaaatacct ggtgacttga aagagtatta tgctggtggc ctttcataaa ggttactgaa
     6301 ctttaatcag gggtgagatg aatgaatgtg aataggccta tatactattt tttttaatta
     6361 aaaaaaaaaa aaacagggca cttgatttaa gaacaaacct gtttaaaggg caggatcagg
     6421 gggaagacag ccttactagg ttggaatctg aatgagattc ttgctggaca aggctgaatt
     6481 atggatgcaa aagccaagca aacttcccag acctggaagt gtcttgtgtt cccttgggtc
     6541 tcttgagatc tccagtattt aaaagtagca gttattgcca tgaactctca aataaaaatg
     6601 ctcctgcctc ttacttgtga atataatcaa catgcagcca gtacatcagg accgcagttg
     6661 ctgaattcat ttacctagtt cccctttaca gcgactatgg acaagaacct ttcagttggt
     6721 tttgtcattc ttggccagat ttaaccaaga aatttctttc actatcagcc tcattttctc
     6781 aggcatgctt acagggacaa agttgttttg agttgagttc tcagcaaata agcattaata
     6841 tcaaactgtg ctaaacttgt ctcacttggt ataagaactt cagcataaag agattagttt
     6901 atcctttgac accaagtctt ttttttttct ttgagacaga gtcttgccct gtcacccagg
     6961 ctggaatgca ttggtgtgat ctcagctcag tcactgcaac ctccgcctcc ccggttcaag
     7021 caattctcct gcctcaccct gcttagtagt tgggattaca ggcatgtgct accacactgg
     7081 gctaattttt gtatatttat tagagacagg gttttgctat gttggccagg ctggtctcaa
     7141 actcctgacc tcaggtgatc cacctgcctc tgcctcccaa agtgctgaga ttacaggtgt
     7201 aagccactgc gcctggcccc cctttttttt ttctttaaaa aaaaaaaaaa gcttggggga
     7261 ttaagtaccc taagatagtg gtctctccca tcagttcaat aatgctgtac agaacctctg
     7321 gataaaaaag ctgttattta caccataatt gtgatgatgg atttgactcc tcaaaaccat
     7381 ctctcccctt tttgcccttt ccaaaaatca aaggcttttg tctccatgag ttttacctag
     7441 tagggattgt agttgtcact ggtctagagt tccaatacat tttatatcag gaacctcagt
     7501 gagctaccca gaattggaac ttaacatggc ccagcaacta ggagaagaag gcaaaccttg
     7561 agaaagctga gccctcagga tagcacagtc tcacccttgt ttaaaatcaa gtcaggctag
     7621 cctttcagtc actctggttt tgtaatgatt tgccttttag gcctcaggcc atttgatttc
     7681 tgcaatagtt ttctgttctt ctcctacagg aatacatccc tgtcctttcg gttgtaacta
     7741 ttactaaaac tagggctatg caaatgacct ggttaccatg taatgaacct tgtgtactta
     7801 ttttgagaga acaatatgta taggatatgt tgaggggcag aaagaaagac atcaaaaact
     7861 ggaactattt taggtggcaa attgtaacgc aaaaaacaaa agtatacctt attttgtata
     7921 cgatgtacac ttgggacaga gttttctaat atgttgccaa tgtttttgta gtgtcaccac
     7981 aggtcttttc tgaagtgttt ttcccatttg ttatagagta ttaatgactt agcgtaatta
     8041 agccctcaag tatgtgtgag agagcgcgtg tgagaaaata caaagccata gatattgatt
     8101 tacttcactg acctgtgtaa ctttatgtct gggtttcgcc atccaagata gattgtttta
     8161 tagagagtgt caccaaagac tttttccttc caatttatag aagaaaaaaa aaagaaatta
     8221 ttaataaatg acatgacttt tca
//
