LOCUS       NM_175876               5100 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens exocyst complex component 8 (EXOC8), mRNA.
ACCESSION   NM_175876
VERSION     NM_175876.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 5100)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5100)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 5100)
  AUTHORS   Hazelett,C.C. and Yeaman,C.
  TITLE     Sec5 and Exo84 mediate distinct aspects of RalA-dependent cell
            polarization
  JOURNAL   PLoS One 7 (6), e39602 (2012)
   PUBMED   22761837
  REMARK    GeneRIF: We identified interactions between RalA and its effectors
            sec5 and exo84 in the Exocyst complex as directly necessary for
            migration and invasion of prostate cancer tumor cells.
REFERENCE   6  (bases 1 to 5100)
  AUTHORS   Moskalenko,S., Tong,C., Rosse,C., Mirey,G., Formstecher,E.,
            Daviet,L., Camonis,J. and White,M.A.
  TITLE     Ral GTPases regulate exocyst assembly through dual subunit
            interactions
  JOURNAL   J Biol Chem 278 (51), 51743-51748 (2003)
   PUBMED   14525976
  REMARK    GeneRIF: evidence that mammalian exocyst components are present as
            distinct subcomplexes on vesicles and the plasma membrane and that
            Ral GTPases regulate the assembly interface of a full octameric
            exocyst complex through interaction with Sec5 and Exo84
REFERENCE   7  (bases 1 to 5100)
  AUTHORS   Inoue,M., Chang,L., Hwang,J., Chiang,S.H. and Saltiel,A.R.
  TITLE     The exocyst complex is required for targeting of Glut4 to the
            plasma membrane by insulin
  JOURNAL   Nature 422 (6932), 629-633 (2003)
   PUBMED   12687004
REFERENCE   8  (bases 1 to 5100)
  AUTHORS   Brymora,A., Valova,V.A., Larsen,M.R., Roufogalis,B.D. and
            Robinson,P.J.
  TITLE     The brain exocyst complex interacts with RalA in a GTP-dependent
            manner: identification of a novel mammalian Sec3 gene and a second
            Sec15 gene
  JOURNAL   J Biol Chem 276 (32), 29792-29797 (2001)
   PUBMED   11406615
REFERENCE   9  (bases 1 to 5100)
  AUTHORS   Hsu,S.C., Hazuka,C.D., Roth,R., Foletti,D.L., Heuser,J. and
            Scheller,R.H.
  TITLE     Subunit composition, protein interactions, and structures of the
            mammalian brain sec6/8 complex and septin filaments
  JOURNAL   Neuron 20 (6), 1111-1122 (1998)
   PUBMED   9655500
REFERENCE   10 (bases 1 to 5100)
  AUTHORS   Kee,Y., Yoo,J.S., Hazuka,C.D., Peterson,K.E., Hsu,S.C. and
            Scheller,R.H.
  TITLE     Subunit structure of the mammalian exocyst complex
  JOURNAL   Proc Natl Acad Sci U S A 94 (26), 14438-14443 (1997)
   PUBMED   9405631
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB442221.1, AK096460.1,
            BC035763.1, DA884148.1 and CA775845.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_175876.4.
            
            Summary: This gene encodes a component of the exocyst complex, an
            evolutionarily conserved multi-protein complex that plays a
            critical role in vesicular trafficking and the secretory pathway by
            targeting post-Golgi vesicles to the plasma membrane. This protein
            is a target of activated Ral subfamily of GTPases and thereby
            regulates exocytosis by tethering vesicles to the plasma membrane.
            Mutations in this gene may be related to Joubert syndrome.
            [provided by RefSeq, Sep 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: AK096460.1, BC035763.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000366645.1/ ENSP00000355605.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-209               DB442221.1         22-230
            210-2426            AK096460.1         210-2426
            2427-2826           BC035763.1         2393-2792
            2827-2949           DA884148.1         315-437
            2950-4645           BC035763.1         2916-4611
            4646-5100           CA775845.1         20-474              c
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q42.2"
     gene            1..5100
                     /gene="EXOC8"
                     /gene_synonym="EXO84; Exo84p; NEDMISB; SEC84"
                     /note="exocyst complex component 8"
                     /db_xref="GeneID:149371"
                     /db_xref="HGNC:HGNC:24659"
                     /db_xref="MIM:615283"
     exon            1..5100
                     /gene="EXOC8"
                     /gene_synonym="EXO84; Exo84p; NEDMISB; SEC84"
                     /inference="alignment:Splign:2.1.0"
     CDS             108..2285
                     /gene="EXOC8"
                     /gene_synonym="EXO84; Exo84p; NEDMISB; SEC84"
                     /note="exocyst complex 84 kDa subunit"
                     /codon_start=1
                     /product="exocyst complex component 8"
                     /protein_id="NP_787072.2"
                     /db_xref="CCDS:CCDS1593.1"
                     /db_xref="GeneID:149371"
                     /db_xref="HGNC:HGNC:24659"
                     /db_xref="MIM:615283"
                     /translation="MAMAMSDSGASRLRRQLESGGFEARLYVKQLSQQSDGDRDLQEH
                     RQRIQALAEETAQNLKRNVYQNYRQFIETAREISYLESEMYQLSHLLTEQKSSLESIP
                     LTLLPAAAAAGAAAASGGEEGVGGAGGRDHLRGQAGFFSTPGGASRDGSGPGEEGKQR
                     TLTTLLEKVEGCRHLLETPGQYLVYNGDLVEYDADHMAQLQRVHGFLMNDCLLVATWL
                     PQRRGMYRYNALYSLDGLAVVNVKDNPPMKDMFKLLMFPESRIFQAENAKIKREWLEV
                     LEDTKRALSEKRRREQEEAAAPRGPPQVTSKATNPFEDDEEEEPAVPEVEEEKVDLSM
                     EWIQELPEDLDVCIAQRDFEGAVDLLDKLNHYLEDKPSPPPVKELRAKVEERVRQLTE
                     VLVFELSPDRSLRGGPKATRRAVSQLIRLGQCTKACELFLRNRAAAVHTAIRQLRIEG
                     ATLLYIHKLCHVFFTSLLETAREFEIDFAGTDSGCYSAFVVWARSAMGMFVDAFSKQV
                     FDSKESLSTAAECVKVAKEHCQQLGDIGLDLTFIIHALLVKDIQGALHSYKEIIIEAT
                     KHRNSEEMWRRMNLMTPEALGKLKEEMKSCGVSNFEQYTGDDCWVNLSYTVVAFTKQT
                     MGFLEEALKLYFPELHMVLLESLVEIILVAVQHVDYSLRCEQDPEKKAFIRQNASFLY
                     ETVLPVVEKRFEEGVGKPAKQLQDLRNASRLIRVNPESTTSVV"
     regulatory      5071..5076
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EXOC8"
                     /gene_synonym="EXO84; Exo84p; NEDMISB; SEC84"
                     /note="hexamer: AATAAA"
     polyA_site      5100
                     /gene="EXOC8"
                     /gene_synonym="EXO84; Exo84p; NEDMISB; SEC84"
                     /note="major polyA site"
ORIGIN      
        1 attcctgctt cctcagccca ggagctcggt tggccttggg tgggtctaga cagccgcggc
       61 ggctgcggcg ccgatagtga cagtgagtgc gtgagacccg gagagaaatg gcgatggcga
      121 tgtcggacag tggggcgagc cgcctgcgtc ggcagctgga gtcagggggt tttgaggcgc
      181 ggctgtacgt gaagcagctc tcgcagcagt cggatgggga ccgggacctc caggagcacc
      241 ggcagcgcat ccaggcgctg gcggaggaga cggcgcagaa cctgaagcgc aacgtctacc
      301 agaactaccg gcagttcata gagacggccc gcgagatctc ctacctggag agcgagatgt
      361 accagctcag ccatttgctg accgagcaga aaagcagcct ggagagcatc ccgcttacgt
      421 tgctgcctgc cgctgctgcc gccggagccg ccgccgcctc tggaggggag gagggagtcg
      481 gtggggcggg gggccgagac cacctccgag gccaggccgg ctttttctcc acccccgggg
      541 gtgcctcccg cgacggctcc ggtccaggcg aggaaggaaa gcagcgcact ctcaccaccc
      601 tgcttgagaa ggtggaaggc tgcaggcatc tgctggagac gccgggacag tacttggtgt
      661 acaatgggga cctagtggaa tacgatgcgg accacatggc ccaactgcag cgggtgcacg
      721 gctttctcat gaacgattgc ttgttggtgg ctacctggct gcctcagcgg cgtgggatgt
      781 atcgctacaa cgctctctat tccctagatg gtttggccgt agtcaatgtc aaggacaacc
      841 cgcccatgaa ggacatgttc aagctgctta tgttccccga gagccgtatt ttccaggccg
      901 aaaatgctaa aatcaaacga gagtggctgg aagtgctgga ggacaccaag agggccctca
      961 gtgagaaaag gcgaagggag caggaggagg cagcggcccc tcgagggcca ccccaagtga
     1021 cttccaaggc cactaaccca tttgaggatg acgaagaaga agaaccagct gttcctgagg
     1081 tagaggaaga gaaggtggac ctctccatgg aatggatcca ggagttacct gaagacctgg
     1141 atgtctgcat tgcgcagaga gactttgaag gggcggttga cctgctggat aaattgaacc
     1201 attacctgga agataaacct agcccacctc ctgtaaaaga actaagggcc aaagtggagg
     1261 agcgagttcg acagctcact gaggtgctag ttttcgaact ctccccagat cgttccctga
     1321 gaggtggtcc gaaggctact cgcagagcag tttcgcaact gatccggctg ggccagtgca
     1381 cgaaggcctg tgagctattt ttgagaaaca gggcagccgc tgttcatact gcaattcgtc
     1441 agcttcgcat cgaaggtgcc actttactct atattcataa gctgtgccat gtcttcttta
     1501 ccagccttct cgagactgca agagaatttg agatcgattt tgcaggcact gacagcggct
     1561 gctactctgc ctttgtggtc tgggcaagat cagccatggg catgttcgtg gatgctttta
     1621 gcaagcaggt gtttgatagt aaggagagcc tctctacagc agctgagtgt gtaaaagtgg
     1681 ctaaggagca ttgccagcaa ctgggtgata tcggactgga tctcaccttc atcatccatg
     1741 cccttctggt gaaagacatc caaggggcct tgcacagtta caaagaaatc atcattgaag
     1801 ccactaaaca tcgcaactct gaagagatgt ggaggaggat gaacttgatg acgccagaag
     1861 ccctgggtaa gctcaaagaa gagatgaaaa gttgtggggt aagtaacttt gagcagtaca
     1921 caggggatga ctgctgggtg aacctaagtt acacagtggt tgctttcacc aaacagacca
     1981 tgggcttctt ggaagaggcc ctgaagctgt atttcccaga gctgcacatg gtacttttgg
     2041 agagcctggt ggaaatcatt ttggttgctg ttcagcatgt ggattatagt cttcgatgtg
     2101 agcaggatcc agagaagaaa gcttttatca gacagaatgc atccttttta tatgaaacag
     2161 tcctccctgt ggtggagaaa aggtttgaag aaggtgtggg gaaacctgcc aagcaactcc
     2221 aagatctgag gaatgcatct agacttattc gtgtgaatcc tgaaagtaca acatcagtgg
     2281 tctaatgctt gggtctgttt atatgtgtat atatgcagag agagagctta tatattattt
     2341 atatttatat taagttgtat tagcatactc tatagtttca aacacaactt gaaaattaaa
     2401 agtgccctct taaaaataca aaaatcaaaa agaggaaaat aagttaaatt aagcccaagt
     2461 aacaaaaata ctggaattat taaaacgtat agtatgctag ctatcctttt aaattatgct
     2521 aattctcttc ttctgaaatt atggtcacac tatatactat agcatttcgg ttttatcctt
     2581 tgataaaact tttctttttt cttttttttt tttgagacag ggtctcaccc cgtcgcacag
     2641 gctggagtgc agtggcaaag tctcgactca ctgcagcctt gacctcccgg gcccaggtag
     2701 tcctcccacc tcagcctccc atgtagcagg aaccacaggc aggcaccacc atacccagct
     2761 aatttttgta ttttttgtag agatggggtt tcgccatgtt gcccaggcta gtcttttctt
     2821 tcttcttttt ttttttcccc acagtatata ttatacagca gtcataatat ctataaatac
     2881 atagagttta tgttgtgaag tttcccagtt cactgaaatg ttaggtttcc taaagggtac
     2941 agtgccgtat aaaacaacct gcctcatata tttctcctca aaacgttgga ctatttggga
     3001 aaaggaaaag agttgggaaa attggtttta aggtaagttt tagtcaaaag aattctttct
     3061 tgaaactagc tggtttgtgg attcagatac tctgatcctt acagaatcca agaggaagct
     3121 ttcataaaaa caattcagca aatatttcca atataatttg aatggctaat tttcagttgc
     3181 taattaatta gcagctttgt aatacttgat ttgggagcat ttacttggaa atcctaagga
     3241 ctataataaa agttttcaac atatttctaa attgtgtgag tttccagctg tagcttttgt
     3301 ctttgtcaca ttttaaaaaa taataagcaa gacacattgg ggacactggc agcagttgcc
     3361 aggttttagc tgccaccgct tcagtatgag atatagctgt cccatcttcc cccattcagg
     3421 gtaggagata tagtgaccca gacttcatgc aaatggaaaa aaagttttaa ctgaaatatt
     3481 tatttagatt tcagggtcta gatggatggg aaagtagaaa aacatatgca aatctcagtg
     3541 ttctcactat gaccactctg agcagagatt tggttttgtt tccttttgta acaaagtgaa
     3601 aacaggtgag acaatgtgcc caaaacaaag ggaagaagag aaccttctgt gactcctaaa
     3661 atgttccatg ctgcattttt gtttcatttt ttatttttcc ttgctttgtt tttaaacatg
     3721 aatataatgc ttacttcaaa ttgtttagta aaacaaaata actaaagaaa tgtgagcttc
     3781 ccaaggtttc taaactatcg ctgttgtata ttctatagcg ttccttattc tttgagggaa
     3841 actgtgcttg ctgtgatcca ttttgtctct agcttctagt tgtgattctt gtccataagc
     3901 accaaatttg atgcccatga tttcaaaagg tcatttcttt tatctgaatg aaaatggtgg
     3961 tactaagact gtgaaaatta tgtgaaacct aaagtagttg ccaaagtggc tcagggttgt
     4021 aaaattcatt gacttaatta ttcatgtgcc agatcaaccc ctttattttc tctttagctg
     4081 tgcatattta aaatattgga aagtatcaga tttacagatt ttctttgact aatttttttc
     4141 acataacttt aggattttcg aaagttgtaa ccataactgg atatcttagc tgagcaaagg
     4201 cggttataat ttgtcttttt aagatcactg gaaattgata aaattttgtg ataattatga
     4261 ttattctgtg ccatttacag tttctaatac tatactgtat gaaatatgta taaatatatg
     4321 atgctgagtc tgtggaatga tacttctgaa atcaaaattc ctcataaggc atgaagttgt
     4381 aaaaacttga atgtgtatag ttagatattt aaatggttgc ttcttcatag aattgtctgc
     4441 tttttaaaac tggaagtaca ggattttctt caggtaaaat ctgtgtgttc caattacagt
     4501 tgtagctgaa ggaagtatgc tttggtgagt caattagtat gggaacttga ctaaagaccc
     4561 ccagtgttgt aacgtacctt tgtacccaga caaaacaatt atgttacatt cctcaaagtg
     4621 gcatgggctt tcttctctaa ttcttctgtt ttattagacc caagacaagt tctaaaaatt
     4681 gaatgcaatg agagattgtc cagaaatgta atatatacta aaatatacca cttaagcatt
     4741 gattgccttt tcttgtttgc ttcaagaata taaaacttgt tacttgagct tggaatcatg
     4801 ggcttgattg aattaattac tcttggggaa aaaagacacc ttgtggcatt aagtcttgct
     4861 ttggttaaag ccttatttca cataattgct aaaaactcat ttttgtttaa tatactacct
     4921 atagtttaat tatcggcact tgtattttgt aacttgatat cttacctagg attgggaatt
     4981 tgggacatga catgtactat aaaagtcagt ctatgtacat actgcttatt gatgtgctgt
     5041 gatatgaggg aatctgaaat gtttcataaa aataaagctt aaaaattgtc ttacactgga
//
