LOCUS       NM_018638               7063 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens ethanolamine kinase 1 (ETNK1), transcript variant 1,
            mRNA.
ACCESSION   NM_018638
VERSION     NM_018638.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Shuai,W., Zuo,Z., Li,N., Garces,S., Jelloul,F.Z., Ok,C.Y., Li,S.,
            Xu,J., You,M.J., Wang,W., Rehder,C., Jabbour,E.J., Patel,K.P.,
            Medeiros,L.J. and Yin,C.C.
  TITLE     ETNK1 mutation occurs in a wide spectrum of myeloid neoplasms and
            is not specific for atypical chronic myeloid leukemia
  JOURNAL   Cancer 129 (6), 878-889 (2023)
   PUBMED   36583229
  REMARK    GeneRIF: ETNK1 mutation occurs in a wide spectrum of myeloid
            neoplasms and is not specific for atypical chronic myeloid
            leukemia.
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Fontana,D., Mauri,M., Renso,R., Docci,M., Crespiatico,I.,
            Rost,L.M., Jang,M., Niro,A., D'Aliberti,D., Massimino,L.,
            Bertagna,M., Zambrotta,G., Bossi,M., Citterio,S., Crescenzi,B.,
            Fanelli,F., Cassina,V., Corti,R., Salerno,D., Nardo,L.,
            Chinello,C., Mantegazza,F., Mecucci,C., Magni,F., Cavaletti,G.,
            Bruheim,P., Rea,D., Larsen,S., Gambacorti-Passerini,C. and
            Piazza,R.
  TITLE     ETNK1 mutations induce a mutator phenotype that can be reverted
            with phosphoethanolamine
  JOURNAL   Nat Commun 11 (1), 5938 (2020)
   PUBMED   33230096
  REMARK    GeneRIF: ETNK1 mutations induce a mutator phenotype that can be
            reverted with phosphoethanolamine.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7063)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 7063)
  AUTHORS   Li,L., Mou,Y.P., Wang,Y.Y., Wang,H.J. and Mou,X.Z.
  TITLE     miR-199a-3p targets ETNK1 to promote invasion and migration in
            gastric cancer cells and is associated with poor prognosis
  JOURNAL   Pathol Res Pract 215 (9), 152511 (2019)
   PUBMED   31255331
  REMARK    GeneRIF: miR-199a-3p regulates the invasion and migration of
            gastric cancer cells by targeting ETNK1
REFERENCE   5  (bases 1 to 7063)
  AUTHORS   Lasho,T.L., Finke,C.M., Zblewski,D., Patnaik,M., Ketterling,R.P.,
            Chen,D., Hanson,C.A., Tefferi,A. and Pardanani,A.
  TITLE     Novel recurrent mutations in ethanolamine kinase 1 (ETNK1) gene in
            systemic mastocytosis with eosinophilia and chronic myelomonocytic
            leukemia
  JOURNAL   Blood Cancer J 5 (1), e275 (2015)
   PUBMED   25615281
  REMARK    GeneRIF: we identified novel somatic missense ETNK1 mutations that
            were most frequent in systemic mastocytosis with eosinophilia and
            chronic myelomonocytic leukemia
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7063)
  AUTHORS   Bailey,S.D., Xie,C., Do,R., Montpetit,A., Diaz,R., Mohan,V.,
            Keavney,B., Yusuf,S., Gerstein,H.C., Engert,J.C. and Anand,S.
  CONSRTM   DREAM investigators
  TITLE     Variation at the NFATC2 locus increases the risk of
            thiazolidinedione-induced edema in the Diabetes REduction
            Assessment with ramipril and rosiglitazone Medication (DREAM) study
  JOURNAL   Diabetes Care 33 (10), 2250-2253 (2010)
   PUBMED   20628086
  REMARK    GeneRIF: Observational study of gene-disease association,
            gene-environment interaction, and pharmacogenomic / toxicogenomic.
            (HuGE Navigator)
REFERENCE   7  (bases 1 to 7063)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   8  (bases 1 to 7063)
  AUTHORS   Talmud,P.J., Drenos,F., Shah,S., Shah,T., Palmen,J., Verzilli,C.,
            Gaunt,T.R., Pallas,J., Lovering,R., Li,K., Casas,J.P., Sofat,R.,
            Kumari,M., Rodriguez,S., Johnson,T., Newhouse,S.J., Dominiczak,A.,
            Samani,N.J., Caulfield,M., Sever,P., Stanton,A., Shields,D.C.,
            Padmanabhan,S., Melander,O., Hastie,C., Delles,C., Ebrahim,S.,
            Marmot,M.G., Smith,G.D., Lawlor,D.A., Munroe,P.B., Day,I.N.,
            Kivimaki,M., Whittaker,J., Humphries,S.E. and Hingorani,A.D.
  CONSRTM   ASCOT investigators; NORDIL investigators; BRIGHT Consortium
  TITLE     Gene-centric association signals for lipids and apolipoproteins
            identified via the HumanCVD BeadChip
  JOURNAL   Am J Hum Genet 85 (5), 628-642 (2009)
   PUBMED   19913121
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 7063)
  AUTHORS   Zafarana,G., Gillis,A.J., van Gurp,R.J., Olsson,P.G., Elstrodt,F.,
            Stoop,H., Millan,J.L., Oosterhuis,J.W. and Looijenga,L.H.
  TITLE     Coamplification of DAD-R, SOX5, and EKI1 in human testicular
            seminomas, with specific overexpression of DAD-R, correlates with
            reduced levels of apoptosis and earlier clinical manifestation
  JOURNAL   Cancer Res 62 (6), 1822-1831 (2002)
   PUBMED   11912161
  REMARK    GeneRIF: DAD-R, SOX5, and EKI1 map within region of chromosome 12p
            whose duplication is related to reduced apoptosis in human
            testicular seminomas.
REFERENCE   10 (bases 1 to 7063)
  AUTHORS   Lykidis,A., Wang,J., Karim,M.A. and Jackowski,S.
  TITLE     Overexpression of a mammalian ethanolamine-specific kinase
            accelerates the CDP-ethanolamine pathway
  JOURNAL   J Biol Chem 276 (3), 2174-2179 (2001)
   PUBMED   11044454
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC087241.11.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Apr 19, 2019 this sequence version replaced NM_018638.4.
            
            Summary: This gene encodes an ethanolamine kinase, which functions
            in the first committed step of the phosphatidylethanolamine
            synthesis pathway. This cytosolic enzyme is specific for
            ethanolamine and exhibits negligible kinase activity on choline.
            Alternative splicing results in multiple transcript variants
            encoding distinct isoforms. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (A).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.51183.1,
                                           SRR1660807.153127.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000266517.9/ ENSP00000266517.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-416               AC087241.11        102574-102989       c
            417-676             AC087241.11        84138-84397         c
            677-817             AC087241.11        69006-69146         c
            818-960             AC087241.11        66955-67097         c
            961-1044            AC087241.11        56805-56888         c
            1045-1205           AC087241.11        54500-54660         c
            1206-1279           AC087241.11        43604-43677         c
            1280-7063           AC087241.11        37495-43278         c
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p12.1"
     gene            1..7063
                     /gene="ETNK1"
                     /gene_synonym="EKI; EKI 1; EKI1; Nbla10396"
                     /note="ethanolamine kinase 1"
                     /db_xref="GeneID:55500"
                     /db_xref="HGNC:HGNC:24649"
                     /db_xref="MIM:609858"
     exon            1..416
                     /gene="ETNK1"
                     /gene_synonym="EKI; EKI 1; EKI1; Nbla10396"
                     /inference="alignment:Splign:2.1.0"
     CDS             261..1352
                     /gene="ETNK1"
                     /gene_synonym="EKI; EKI 1; EKI1; Nbla10396"
                     /EC_number="2.7.1.82"
                     /note="isoform A is encoded by transcript variant 1;
                     putative protein product of Nbla10396"
                     /codon_start=1
                     /product="ethanolamine kinase 1 isoform A"
                     /protein_id="NP_061108.3"
                     /db_xref="CCDS:CCDS8698.2"
                     /db_xref="GeneID:55500"
                     /db_xref="HGNC:HGNC:24649"
                     /db_xref="MIM:609858"
                     /translation="MANYIHVPPGSPEVPKLNVTVQDQEEHRCREGALSLLQHLRPHW
                     DPQEVTLQLFTDGITNKLIGCYVGNTMEDVVLVRIYGNKTELLVDRDEEVKSFRVLQA
                     HGCAPQLYCTFNNGLCYEFIQGEALDPKHVCNPAIFRLIARQLAKIHAIHAHNGWIPK
                     SNLWLKMGKYFSLIPTGFADEDINKRFLSDIPSSQILQEEMTWMKEILSNLGSPVVLC
                     HNDLLCKNIIYNEKQGDVQFIDYEYSGYNYLAYDIGNHFNEFAGVSDVDYSLYPDREL
                     QSQWLRAYLEAYKEFKGFGTEVTEKEVEILFIQVNQFALASHFFWGLWALIQAKYSTI
                     EFDFLGYAIVRFNQYFKMKPEVTALKVPE"
     exon            417..676
                     /gene="ETNK1"
                     /gene_synonym="EKI; EKI 1; EKI1; Nbla10396"
                     /inference="alignment:Splign:2.1.0"
     exon            677..817
                     /gene="ETNK1"
                     /gene_synonym="EKI; EKI 1; EKI1; Nbla10396"
                     /inference="alignment:Splign:2.1.0"
     exon            818..960
                     /gene="ETNK1"
                     /gene_synonym="EKI; EKI 1; EKI1; Nbla10396"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1044
                     /gene="ETNK1"
                     /gene_synonym="EKI; EKI 1; EKI1; Nbla10396"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1205
                     /gene="ETNK1"
                     /gene_synonym="EKI; EKI 1; EKI1; Nbla10396"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1279
                     /gene="ETNK1"
                     /gene_synonym="EKI; EKI 1; EKI1; Nbla10396"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..7063
                     /gene="ETNK1"
                     /gene_synonym="EKI; EKI 1; EKI1; Nbla10396"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2089..2094
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ETNK1"
                     /gene_synonym="EKI; EKI 1; EKI1; Nbla10396"
                     /note="hexamer: AATAAT"
     polyA_site      2110
                     /gene="ETNK1"
                     /gene_synonym="EKI; EKI 1; EKI1; Nbla10396"
                     /note="major polyA site"
     regulatory      7041..7046
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ETNK1"
                     /gene_synonym="EKI; EKI 1; EKI1; Nbla10396"
                     /note="hexamer: AATAAA"
     polyA_site      7063
                     /gene="ETNK1"
                     /gene_synonym="EKI; EKI 1; EKI1; Nbla10396"
                     /note="major polyA site"
ORIGIN      
        1 gcggccgccc gcggtccagc tccgacaaca ggaattttct ccgagagcgg gccgggctca
       61 gttcagctgc tgtccagacc cggatcggca acagtgccgc ctccagacgt tctcctgccg
      121 ctcgcccgcc cgtcccagcg cccccagccc tcccgcgagg gcgccccggg acggaaggat
      181 ccaccagtct gtcggcgccc gccgttctcg tggtcgccgt cgccgtcgtc gtggtggtag
      241 tctccgccgt cgcctgggcc atggccaatt acatccacgt ccctcccggc tccccggagg
      301 tgcccaagct gaacgtcacc gttcaggatc aggaggagca tcgctgccgg gagggggccc
      361 tgagcctcct gcaacacctg cggcctcact gggaccccca ggaggtgacc ctgcagctct
      421 tcacagatgg aatcacaaat aaacttattg gctgttacgt gggaaacacc atggaggatg
      481 tagtcctggt gagaatttat ggcaataaga ctgagttatt agtcgatcga gatgaggaag
      541 taaagagttt tcgagtgttg caggctcatg ggtgtgcacc acaactctac tgtaccttca
      601 ataatggact atgctatgaa tttatacaag gagaagcact ggatccaaag catgtctgca
      661 acccagccat tttcaggcta atagctcgtc agcttgctaa aatccatgct attcatgcac
      721 acaatggctg gatccccaaa tctaatcttt ggctaaagat gggaaagtat ttctctctca
      781 ttcccacagg atttgcagat gaagacatta ataaaaggtt cctaagtgat atcccaagct
      841 ctcagattct ccaggaagag atgacttgga tgaaggagat tctttccaac ctgggctcac
      901 ctgttgtgct ttgccataat gacctattgt gtaagaatat aatctacaat gagaaacaag
      961 gtgatgtaca gttcattgat tatgaatatt ctggatacaa ctacctggca tatgatattg
     1021 gaaatcattt caatgaattt gcaggtgtga gtgatgtaga ctatagtctg tatccagata
     1081 gagaactaca gagtcagtgg ctgcgtgctt accttgaagc ctacaaagaa tttaagggct
     1141 ttgggactga agttactgaa aaggaggtag aaatactctt cattcaagtc aatcagtttg
     1201 cattggcttc tcatttcttt tggggattgt gggctttgat tcaagccaaa tactccacta
     1261 ttgagtttga tttccttggg tatgcaattg ttcgttttaa ccagtacttt aaaatgaagc
     1321 ctgaggttac tgcattaaaa gtgcctgagt aaagaagaga tttaattatt ctccagtagc
     1381 tgagcaatgc ttgtgaatct tttcttaaga aatcccaaaa agccaatatt agttaaaatt
     1441 ctgttgttta atttggttat cttgctttat aaattatgcc tctaaacaat caaatctatt
     1501 tttgaaatag actgaatgat gtcaagaaat atacctactg ctatccgtat gtggtggatt
     1561 agaaatgtgt taaatctgca aaaggtataa agatgtcagt ttaatttctt tgataattta
     1621 acctatgttg tatgtgaatt atttattata aacttagcac gattctgtga ctgtttttct
     1681 ctgtttcacg ttcgttgagt gtaagcaatg aaaatgtccc aaataagttt tttaagtttt
     1741 actttaataa gattaatttc agtaaacatt ctagttgttc agtgtaacct ttttatcttg
     1801 atgcattgta agtaaaatga atcatttact cttgaaatgc cagtcattga ctgatgtaga
     1861 taatttagga ttttcatata aaaatagctg tttaggaagg tgaaatacat tcactgtctc
     1921 tgttggtggt acatcttgtt gaattcaata ttagaaagta tttctttttg gggtaatata
     1981 acttagaatt aaatccctgt ttctctatgt agtctggcag tataaatata aatatttacc
     2041 atataatctt ggaataagta ttagttaatg ttaccaaaat ctgtattaaa taatgttttc
     2101 aaatgctaaa tatggtcgtt actattttca gttttaaaaa ttttatagta tcaaattgtt
     2161 tctaaccaaa aatttccttt tacttctaga gatgctttat gtttttgatt attaaatagt
     2221 cactagtatt gctaattttt gaaacaaata cacaaaattt atcatatgta gaaataagaa
     2281 aatattgttt aggttaatta ttttttacct atccagagtc attccttaca tttcagtttc
     2341 atgtttcatt cctcacattt tagtttcatg ttttagtttg attctatgtt tttagactga
     2401 tagcaaatga ttacttgata catgtaagtt cagcgttata tgttgaggca gttatataat
     2461 taaataagga acacttaggg cattgatctt gtacacttaa aatctgacaa atgacaaaat
     2521 gtgaattaag cataaatagt catggcagga gggcttttgt ttatttctct tgtttttgtt
     2581 taactgtatt tttaattttg tctggtggta agagggaggt aattattgta tggaaagaag
     2641 ttagatcttt cagatagata aataggcttc aggttttgaa gtctgctctg tgtagttgag
     2701 cagtcatggt taactttact tagaaaatta gtctgacaag ctttgcactt cggtcacata
     2761 agtgcccatg tacaaagttg gcttactgga tctgcatatt caaaatatgc aactacaaat
     2821 ttatcctctg agaaatcttc tgaggagtct aatgtattcc aaatgactta atattctcag
     2881 acaaatgctt taaaatactt ggattatctc aagatttttg aagctaataa agtaaataga
     2941 aacacaggcc attttaaatg gtaaattcta gttacataaa tataatctta aagtatgttt
     3001 gtatctgtaa cagtgttaga ccagatttgg tttctacctc cccaatgtta taaatgttca
     3061 cttttgtttt tataaaccat attactgtaa gatcaataga actttgccta gttaagtgaa
     3121 tggaaccaat taaacaacta gtagcttttt ataatgttag tgtgaacttg aaaaattgtt
     3181 tttggtggaa tttttgtctg tggttagaac attttcataa aacagataaa gaatgtgtaa
     3241 tactcttaat tttttttttt tttttttttt tttttttgct ttctgccttc taaagtgcaa
     3301 cagtatatat cttgtagaac tgtgggagga gagaagataa tttttctacc ttactccata
     3361 ttcttacttc aaactatttt gaatagaggt tcactgagcc attgctgata gactgtgcat
     3421 agctactagc tgtcacacta ttgtaggttt tttggagaga ttcataggga agtgaatgta
     3481 accaggaata aaggctctca ctttagctct gggactatgc agtggatctt gggaacaaaa
     3541 taagatatct aatctatttt ccccattctg gctagatttt ggttgttgtg atgctcctac
     3601 ttcctgtata ggtttttggg atcatggtgc aggctagtaa taggattcag aattgcttcc
     3661 cttttatatc gaccatgtaa gggattctaa aggagggtaa atctcaaagt gctctgaaat
     3721 catccctcta aaatctgtcc tgtggtgtgc taagatgtct gttgcctttt tatgttaagc
     3781 taaggaactt gaatgcctta cctaataact caactagtag taagctcatt ttgtaaatat
     3841 gaaaatggct gtaagaagtg gcattcatgt atattcaatg ttagtcaata gaccatagtg
     3901 gcctggatgc tttaacaagc caaattccgc aatttttttt ccttttttcc ccttcatact
     3961 tgtatgatct cacctgacca gtgatttctt ttcctcctaa ccccatgcta ccaccatttt
     4021 ctggaaatca aagagatgtg gtagtaaatc atacagtaca cacgatcatc tgagatcaat
     4081 atgagcacaa aactcatcaa gtaggtctgc ctggaatgta tataaaaaat gtaaataaaa
     4141 atttgtaaat tagtgtgaac tgtatcttgc atatgagact gtgtattgtt ttgcattctc
     4201 ttcccttttg tagaaatttt tctgtaagga aatgattcag cttgttggtt ttagagtaaa
     4261 aatagccaac agttgtggct acacagtgag atcacaggaa ctggaaatag ggtttgtcgg
     4321 ctttggttaa tgtcagtact catttattta gcctctcagt gcttaataag acttttcttt
     4381 attttttagt tagccgttag gttttgtgag gttagattcc tggaagcagt gaatttatac
     4441 actgttatat taatagagct ccgttctttg agtcatgatt ccaagctaaa ggaaattaaa
     4501 aatgtaattt aataatttcc tatttttagg gttgttaatt tttttctaca aaaaaacctt
     4561 gaaattttag atatcccaat gtgaatctaa tttccatata tacagaaatt agacaaataa
     4621 taagtcttta gttcaactta agcatatctc aaatgacttc tctaaattta aagttgatca
     4681 tgataggatc ataaaagaca gaaaagactt aagtaatctt gtaatgacaa ttatttccat
     4741 ttttgctgaa ctaaaaatat ttaacttcat aaatatgtta ctacagcttc cagatttaaa
     4801 gaaaaaaagt ttcccccact ctcaattaaa agttagaacc ctccactttt aaaattatac
     4861 aaatatttct tttttacatt acacagaagc cttctgtacc attttacgaa tttctgtctt
     4921 cataatataa gtgaaaatac tgtcatttca attttctgct ttaaattgtt tttaataagc
     4981 attccaaagt gatacagact taagctttta atcaatcagt cattcagttg atagacaaag
     5041 ttagcgatgc tttatgctag gaaacttgtt gacagtaacc tgtgcgactt tatgcagaag
     5101 acaaatgcta gtaattatta tgcacagagg aaaaatcatt ttaagtatgt ggtaaagcag
     5161 cttcatcttt caaaattgat ttgctctggt ttttctttag tccattagat tccagaatgt
     5221 ccttttactg ggaatttagt tatgtattaa gataacctgt tttcagttct ttttgaaaag
     5281 aagacattat ttatattgaa ccaccttatt ttaaaatttt taacttttat ataccacttg
     5341 tgtgattcca gtgtcatgtc ttgggtttga tgtcgttgga cagaaaagtg tatcaattat
     5401 tttaaatgaa tttttcccca tgtttgaggc ttagtctgta aatgtgttgc tgtaacagaa
     5461 aatacttggg tatgcattac ttgaatactt gaaaactgaa attaataaga tgtattacat
     5521 aatgaattag atttctctga acagttttta cactgaaaat cttcatttct ggattgcagt
     5581 ttgaaatgga atgaagacct gaattatttg ggtagaaaaa attatgatag tgcttataag
     5641 aactgtaaac tgttttaaac tattttgtgt ttgacgcatc aaacttcaag ttttttgtaa
     5701 gtttctctcc tgaaattttc tttctcttct atactttatg cacttactat actactgatg
     5761 taataaaaga gcagggttaa aaatattgta tctgtattca ttgtgaatcc tgtagctttt
     5821 ctagttaaca aaaaatcgct ttctaaaata ctcttaatcc cattgttttg gttaacatct
     5881 tacccatttg ttgtatttca aatgccatta atcattttag tacaacacct atgtttataa
     5941 aaatttgaaa acattacata ttgtatttaa aactaattag tgaagagtaa gaaaaaaact
     6001 agccaacaga attgtaggtg atgcattagt taaatttcaa aactcataat aaaggaactt
     6061 tcagagattg gttgaaaccc agtggtatcc ctgtaaatta gctcctgtga ctggaaaaga
     6121 ccccaaaaag gcagtagagg agattagtgt ttacttgctg tggttgtggt gtgctgctac
     6181 ttaattatag gtagtgacac actgaaattc ttatttgtcc aataatctga agtagtttcc
     6241 tatatttatc tgtactaaat tgactataaa ttgagtctgc aaagaggaaa ctttttgact
     6301 gtactgtatt taggagcctt tgtacagctt ggtcaaattt ccatgatatg aagtatttga
     6361 gttttaaaat atactgttat taaaaggaaa aagacatggc cattattcca tgtgcttaaa
     6421 tgataatttc cttattcagt ttcagaagaa aaagaatgaa attgggtaac tgtcattgcg
     6481 ttagctttat gttgaattgg gaaattgtgg cataaagctt aaattcgtgt ttatcaaatg
     6541 tgaaccatag tagtataatg ctgctttgta tataatgtaa gtgctacaaa tagtctcagc
     6601 actgaaaatg tattgatacc tcttaaatga atgcaacttt tgatgtaggt gttttgctat
     6661 gcctcagaaa atatctgtct gagaatttgt taatctgttt gataatgaag atacttcctg
     6721 ttttcttgtt tcatattttc atgttcaaaa tttaagtttt acatttttac tactgttaat
     6781 ttaaataaaa tttgttctgt ggataaaatg aggttggcag tgaagaaaat taaaaacagc
     6841 ctcattcatg taactggtta agtaaaaata cattttcact atgtgttcat aaacttttaa
     6901 tgaagctgtt tgtctttcag ttcaaatata agtgatgttt aggctttatt tctgttaata
     6961 aggcttttta ccattgatta aatgaaggaa tgtatctttt tgaagagatt tatattctgt
     7021 aaataaaaat tcgttgtaac aataaagttg agttctaact aca
//
