LOCUS       NM_001291241            6279 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens estrogen receptor 1 (ESR1), transcript variant 6,
            mRNA.
ACCESSION   NM_001291241
VERSION     NM_001291241.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Liu,X., Matsuyama,Y., Sugiyama,M., Suyama,K., Nose,T.,
            Shimohigashi,M. and Shimohigashi,Y.
  TITLE     The N-terminal activation function AF-1 domain of ERalpha interacts
            directly with the C-terminal AF-2-holding ligand-binding domain to
            recruit the coactivator proteins
  JOURNAL   PLoS One 19 (10), e0312276 (2024)
   PUBMED   39432505
  REMARK    GeneRIF: The N-terminal activation function AF-1 domain of ERalpha
            interacts directly with the C-terminal AF-2-holding ligand-binding
            domain to recruit the coactivator proteins.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Aboelroos,S.A., Segaey,D.G.E., Elgawad,A.K.A., Orabi,M.,
            Mohamed,M.H. and Hassan,N.R.
  TITLE     Association of Estrogen Receptor-alpha and Aryl Hydrocarbon
            Receptor Gene Polymorphisms with Ischemic Stroke in an Egyptian
            Population: A Pilot Study
  JOURNAL   J Mol Neurosci 74 (3), 85 (2024)
   PUBMED   39264466
  REMARK    GeneRIF: Association of Estrogen Receptor-alpha and Aryl
            Hydrocarbon Receptor Gene Polymorphisms with Ischemic Stroke in an
            Egyptian Population: A Pilot Study.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6279)
  AUTHORS   Zhuang,T., Zhang,S., Liu,D., Li,Z., Li,X., Li,J., Yang,P.,
            Zhang,C., Cui,J., Fu,M., Shen,F., Yuan,L., Zhang,Z., Su,P., Zhu,J.
            and Yang,H.
  TITLE     USP36 promotes tumorigenesis and tamoxifen resistance in breast
            cancer by deubiquitinating and stabilizing ERalpha
  JOURNAL   J Exp Clin Cancer Res 43 (1), 249 (2024)
   PUBMED   39215346
  REMARK    GeneRIF: USP36 promotes tumorigenesis and tamoxifen resistance in
            breast cancer by deubiquitinating and stabilizing ERalpha.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6279)
  AUTHORS   Ishii,H., Kobayashi,M., Munetomo,A., Miyamoto,T. and Sakuma,Y.
  TITLE     Novel splicing events and post-transcriptional regulation of human
            estrogen receptor alpha E isoforms
  JOURNAL   J Steroid Biochem Mol Biol 133, 120-128 (2013)
   PUBMED   23032375
  REMARK    GeneRIF: the 5'-UTRs of the E isoforms post-transcriptionally
            control ERalpha expression mainly through translational repression
REFERENCE   5  (bases 1 to 6279)
  AUTHORS   Pentecost,B.T., Song,R., Luo,M., DePasquale,J.A. and Fasco,M.J.
  TITLE     Upstream regions of the estrogen receptor alpha proximal promoter
            transcript regulate ER protein expression through a translational
            mechanism
  JOURNAL   Mol Cell Endocrinol 229 (1-2), 83-94 (2005)
   PUBMED   15607532
REFERENCE   6  (bases 1 to 6279)
  AUTHORS   Kos,M., Denger,S., Reid,G. and Gannon,F.
  TITLE     Upstream open reading frames regulate the translation of the
            multiple mRNA variants of the estrogen receptor alpha
  JOURNAL   J Biol Chem 277 (40), 37131-37138 (2002)
   PUBMED   12147702
  REMARK    GeneRIF: Upstream open reading frames regulate the translation of
            the multiple mRNA variants
REFERENCE   7  (bases 1 to 6279)
  AUTHORS   Ing,N.H., Beekman,J.M., Tsai,S.Y., Tsai,M.J. and O'Malley,B.W.
  TITLE     Members of the steroid hormone receptor superfamily interact with
            TFIIB (S300-II)
  JOURNAL   J Biol Chem 267 (25), 17617-17623 (1992)
   PUBMED   1517211
REFERENCE   8  (bases 1 to 6279)
  AUTHORS   Piva,R., Gambari,R., Zorzato,F., Kumar,L. and del Senno,L.
  TITLE     Analysis of upstream sequences of the human estrogen receptor gene
  JOURNAL   Biochem Biophys Res Commun 183 (3), 996-1002 (1992)
   PUBMED   1567414
REFERENCE   9  (bases 1 to 6279)
  AUTHORS   Keaveney,M., Klug,J. and Gannon,F.
  TITLE     Sequence analysis of the 5' flanking region of the human estrogen
            receptor gene
  JOURNAL   DNA Seq 2 (6), 347-358 (1992)
   PUBMED   1476547
  REMARK    Erratum:[DNA Seq 1992;3(3):201]
REFERENCE   10 (bases 1 to 6279)
  AUTHORS   Keaveney,M., Klug,J., Dawson,M.T., Nestor,P.V., Neilan,J.G.,
            Forde,R.C. and Gannon,F.
  TITLE     Evidence for a previously unidentified upstream exon in the human
            oestrogen receptor gene
  JOURNAL   J Mol Endocrinol 6 (1), 111-115 (1991)
   PUBMED   2015052
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL356311.6, AL590993.14,
            AL049821.6 and AL078582.13.
            
            On Jul 20, 2020 this sequence version replaced NM_001291241.1.
            
            Summary: This gene encodes an estrogen receptor and
            ligand-activated transcription factor. The canonical protein
            contains an N-terminal ligand-independent transactivation domain, a
            central DNA binding domain, a hinge domain, and a C-terminal
            ligand-dependent transactivation domain. The protein localizes to
            the nucleus where it may form either a homodimer or a heterodimer
            with estrogen receptor 2. The protein encoded by this gene
            regulates the transcription of many estrogen-inducible genes that
            play a role in growth, metabolism, sexual development, gestation,
            and other reproductive functions and is expressed in many
            non-reproductive tissues. The receptor encoded by this gene plays a
            key role in breast cancer, endometrial cancer, and osteoporosis.
            This gene is reported to have dozens of transcript variants due to
            the use of alternate promoters and alternative splicing, however,
            the full-length nature of many of these variants remain uncertain.
            [provided by RefSeq, Jul 2020].
            
            Transcript Variant: This variant (6) differs in the 5' UTR and has
            an alternate splice site in the 5' coding region, compared to
            variant 1. The resulting isoform (3) lacks an internal aa in the
            N-terminal region, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK293019.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2156099, SAMEA2467142
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            regulatory uORF :: PMID: 2015052
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-116               AL356311.6         47404-47519
            117-638             AL356311.6         49542-50063
            639-829             AL590993.14        18950-19140
            830-943             AL590993.14        57008-57121
            944-1279            AL590993.14        120526-120861
            1280-1418           AL049821.6         50322-50460
            1419-1552           AL078582.13        12052-12185
            1553-1736           AL078582.13        45446-45629
            1737-6279           AL078582.13        49793-54335
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q25.1-q25.2"
     gene            1..6279
                     /gene="ESR1"
                     /gene_synonym="ER; Era; ESR; ESRA; ESTRR; NR3A1"
                     /note="estrogen receptor 1"
                     /db_xref="GeneID:2099"
                     /db_xref="HGNC:HGNC:3467"
                     /db_xref="MIM:133430"
     exon            1..116
                     /gene="ESR1"
                     /gene_synonym="ER; Era; ESR; ESRA; ESTRR; NR3A1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      21..65
                     /regulatory_class="uORF"
                     /gene="ESR1"
                     /gene_synonym="ER; Era; ESR; ESRA; ESTRR; NR3A1"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:2015052]"
                     /note="this uORF is predicted to encode a 14 aa peptide
                     (PMID:2015052)"
     regulatory      79..135
                     /regulatory_class="uORF"
                     /gene="ESR1"
                     /gene_synonym="ER; Era; ESR; ESRA; ESTRR; NR3A1"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:2015052]"
                     /note="this uORF is predicted to encode a 18 aa peptide
                     (PMID:2015052)"
     exon            117..638
                     /gene="ESR1"
                     /gene_synonym="ER; Era; ESR; ESRA; ESTRR; NR3A1"
                     /inference="alignment:Splign:2.1.0"
     CDS             187..1971
                     /gene="ESR1"
                     /gene_synonym="ER; Era; ESR; ESRA; ESTRR; NR3A1"
                     /note="isoform 3 is encoded by transcript variant 6;
                     ER-alpha; estradiol receptor; nuclear receptor subfamily 3
                     group A member 1; estrogen nuclear receptor alpha;
                     estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha
                     E1-N2-E2-1-2; oestrogen receptor alpha; E2 receptor alpha"
                     /codon_start=1
                     /product="estrogen receptor isoform 3"
                     /protein_id="NP_001278170.1"
                     /db_xref="GeneID:2099"
                     /db_xref="HGNC:HGNC:3467"
                     /db_xref="MIM:133430"
                     /translation="MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYL
                     DSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNS
                     VSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQG
                     GRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHND
                     YMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGIRKDRRGGRMLKHKRQRDDGEGR
                     GEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTR
                     PFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVW
                     RSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSI
                     ILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLL
                     ILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSH
                     LATAGSTSSHSLQKYYITGEAEGFPATV"
     exon            639..829
                     /gene="ESR1"
                     /gene_synonym="ER; Era; ESR; ESRA; ESTRR; NR3A1"
                     /inference="alignment:Splign:2.1.0"
     exon            830..943
                     /gene="ESR1"
                     /gene_synonym="ER; Era; ESR; ESRA; ESTRR; NR3A1"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1279
                     /gene="ESR1"
                     /gene_synonym="ER; Era; ESR; ESRA; ESTRR; NR3A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1418
                     /gene="ESR1"
                     /gene_synonym="ER; Era; ESR; ESRA; ESTRR; NR3A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1552
                     /gene="ESR1"
                     /gene_synonym="ER; Era; ESR; ESRA; ESTRR; NR3A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1736
                     /gene="ESR1"
                     /gene_synonym="ER; Era; ESR; ESRA; ESTRR; NR3A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1737..6279
                     /gene="ESR1"
                     /gene_synonym="ER; Era; ESR; ESRA; ESTRR; NR3A1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6261..6266
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ESR1"
                     /gene_synonym="ER; Era; ESR; ESRA; ESTRR; NR3A1"
                     /note="hexamer: AATAAA"
     polyA_site      6279
                     /gene="ESR1"
                     /gene_synonym="ER; Era; ESR; ESRA; ESTRR; NR3A1"
                     /note="major polyA site"
ORIGIN      
        1 acacactgag ccactcgcac atgcgagcac attccttcct tccttctcac tctctcggcc
       61 cttgacttct acaagcccat ggaacatttc tggaaagacg ttcttgatcc agcagggtgg
      121 cccgccggtt tctgagcctt ctgccctgcg gggacacggt ctgcaccctg cccgcggcca
      181 cggaccatga ccatgaccct ccacaccaaa gcatctggga tggccctact gcatcagatc
      241 caagggaacg agctggagcc cctgaaccgt ccgcagctca agatccccct ggagcggccc
      301 ctgggcgagg tgtacctgga cagcagcaag cccgccgtgt acaactaccc cgagggcgcc
      361 gcctacgagt tcaacgccgc ggccgccgcc aacgcgcagg tctacggtca gaccggcctc
      421 ccctacggcc ccgggtctga ggctgcggcg ttcggctcca acggcctggg gggtttcccc
      481 ccactcaaca gcgtgtctcc gagcccgctg atgctactgc acccgccgcc gcagctgtcg
      541 cctttcctgc agccccacgg ccagcaggtg ccctactacc tggagaacga gcccagcggc
      601 tacacggtgc gcgaggccgg cccgccggca ttctacaggc caaattcaga taatcgacgc
      661 cagggtggca gagaaagatt ggccagtacc aatgacaagg gaagtatggc tatggaatct
      721 gccaaggaga ctcgctactg tgcagtgtgc aatgactatg cttcaggcta ccattatgga
      781 gtctggtcct gtgagggctg caaggccttc ttcaagagaa gtattcaagg acataacgac
      841 tatatgtgtc cagccaccaa ccagtgcacc attgataaaa acaggaggaa gagctgccag
      901 gcctgccggc tccgtaaatg ctacgaagtg ggaatgatga aagggatacg aaaagaccga
      961 agaggaggga gaatgttgaa acacaagcgc cagagagatg atggggaggg caggggtgaa
     1021 gtggggtctg ctggagacat gagagctgcc aacctttggc caagcccgct catgatcaaa
     1081 cgctctaaga agaacagcct ggccttgtcc ctgacggccg accagatggt cagtgccttg
     1141 ttggatgctg agcccccgat actctattcc gagtatgatc ctaccagacc cttcagtgaa
     1201 gcttcgatga tgggcttact gaccaacctg gcagacaggg agctggttca catgatcaac
     1261 tgggcgaaga gggtgccagg ctttgtggat ttgaccctcc atgatcaggt ccaccttcta
     1321 gaatgtgcct ggctagagat cctgatgatt ggtctcgtct ggcgctccat ggagcaccca
     1381 gggaagctac tgtttgctcc taacttgctc ttggacagga accagggaaa atgtgtagag
     1441 ggcatggtgg agatcttcga catgctgctg gctacatcat ctcggttccg catgatgaat
     1501 ctgcagggag aggagtttgt gtgcctcaaa tctattattt tgcttaattc tggagtgtac
     1561 acatttctgt ccagcaccct gaagtctctg gaagagaagg accatatcca ccgagtcctg
     1621 gacaagatca cagacacttt gatccacctg atggccaagg caggcctgac cctgcagcag
     1681 cagcaccagc ggctggccca gctcctcctc atcctctccc acatcaggca catgagtaac
     1741 aaaggcatgg agcatctgta cagcatgaag tgcaagaacg tggtgcccct ctatgacctg
     1801 ctgctggaga tgctggacgc ccaccgccta catgcgccca ctagccgtgg aggggcatcc
     1861 gtggaggaga cggaccaaag ccacttggcc actgcgggct ctacttcatc gcattccttg
     1921 caaaagtatt acatcacggg ggaggcagag ggtttccctg ccacggtctg agagctccct
     1981 ggctcccaca cggttcagat aatccctgct gcattttacc ctcatcatgc accactttag
     2041 ccaaattctg tctcctgcat acactccggc atgcatccaa caccaatggc tttctagatg
     2101 agtggccatt catttgcttg ctcagttctt agtggcacat cttctgtctt ctgttgggaa
     2161 cagccaaagg gattccaagg ctaaatcttt gtaacagctc tctttccccc ttgctatgtt
     2221 actaagcgtg aggattcccg tagctcttca cagctgaact cagtctatgg gttggggctc
     2281 agataactct gtgcatttaa gctacttgta gagacccagg cctggagagt agacattttg
     2341 cctctgataa gcacttttta aatggctcta agaataagcc acagcaaaga atttaaagtg
     2401 gctcctttaa ttggtgactt ggagaaagct aggtcaaggg tttattatag caccctcttg
     2461 tattcctatg gcaatgcatc cttttatgaa agtggtacac cttaaagctt ttatatgact
     2521 gtagcagagt atctggtgat tgtcaattca ttccccctat aggaatacaa ggggcacaca
     2581 gggaaggcag atcccctagt tggcaagact attttaactt gatacactgc agattcagat
     2641 gtgctgaaag ctctgcctct ggctttccgg tcatgggttc cagttaattc atgcctccca
     2701 tggacctatg gagagcagca agttgatctt agttaagtct ccctatatga gggataagtt
     2761 cctgattttt gtttttattt ttgtgttaca aaagaaagcc ctccctccct gaacttgcag
     2821 taaggtcagc ttcaggacct gttccagtgg gcactgtact tggatcttcc cggcgtgtgt
     2881 gtgccttaca caggggtgaa ctgttcactg tggtgatgca tgatgagggt aaatggtagt
     2941 tgaaaggagc aggggccctg gtgttgcatt tagccctggg gcatggagct gaacagtact
     3001 tgtgcaggat tgttgtggct actagagaac aagagggaaa gtagggcaga aactggatac
     3061 agttctgagg cacagccaga cttgctcagg gtggccctgc cacaggctgc agctacctag
     3121 gaacattcct tgcagacccc gcattgccct ttgggggtgc cctgggatcc ctggggtagt
     3181 ccagctcttc ttcatttccc agcgtggccc tggttggaag aagcagctgt cacagctgct
     3241 gtagacagct gtgttcctac aattggccca gcaccctggg gcacgggaga agggtgggga
     3301 ccgttgctgt cactactcag gctgactggg gcctggtcag attacgtatg cccttggtgg
     3361 tttagagata atccaaaatc agggtttggt ttggggaaga aaatcctccc ccttcctccc
     3421 ccgccccgtt ccctaccgcc tccactcctg ccagctcatt tccttcaatt tcctttgacc
     3481 tataggctaa aaaagaaagg ctcattccag ccacagggca gccttccctg ggcctttgct
     3541 tctctagcac aattatgggt tacttccttt ttcttaacaa aaaagaatgt ttgatttcct
     3601 ctgggtgacc ttattgtctg taattgaaac cctattgaga ggtgatgtct gtgttagcca
     3661 atgacccagg tgagctgctc gggcttctct tggtatgtct tgtttggaaa agtggatttc
     3721 attcatttct gattgtccag ttaagtgatc accaaaggac tgagaatctg ggagggcaaa
     3781 aaaaaaaaaa aagtttttat gtgcacttaa atttggggac aattttatgt atctgtgtta
     3841 aggatatgtt taagaacata attcttttgt tgctgtttgt ttaagaagca ccttagtttg
     3901 tttaagaagc accttatata gtataatata tatttttttg aaattacatt gcttgtttat
     3961 cagacaattg aatgtagtaa ttctgttctg gatttaattt gactgggtta acatgcaaaa
     4021 accaaggaaa aatatttagt tttttttttt ttttttgtat acttttcaag ctaccttgtc
     4081 atgtatacag tcatttatgc ctaaagcctg gtgattattc atttaaatga agatcacatt
     4141 tcatatcaac ttttgtatcc acagtagaca aaatagcact aatccagatg cctattgttg
     4201 gatactgaat gacagacaat cttatgtagc aaagattatg cctgaaaagg aaaattattc
     4261 agggcagcta attttgcttt taccaaaata tcagtagtaa tatttttgga cagtagctaa
     4321 tgggtcagtg ggttcttttt aatgtttata cttagatttt cttttaaaaa aattaaaata
     4381 aaacaaaaaa aaatttctag gactagacga tgtaatacca gctaaagcca aacaattata
     4441 cagtggaagg ttttacatta ttcatccaat gtgtttctat tcatgttaag atactactac
     4501 atttgaagtg ggcagagaac atcagatgat tgaaatgttc gcccaggggt ctccagcaac
     4561 tttggaaatc tctttgtatt tttacttgaa gtgccactaa tggacagcag atattttctg
     4621 gctgatgttg gtattgggtg taggaacatg atttaaaaaa aaactcttgc ctctgctttc
     4681 ccccactctg aggcaagtta aaatgtaaaa gatgtgattt atctgggggg ctcaggtatg
     4741 gtggggaagt ggattcagga atctggggaa tggcaaatat attaagaaga gtattgaaag
     4801 tatttggagg aaaatggtta attctgggtg tgcaccaggg ttcagtagag tccacttctg
     4861 ccctggagac cacaaatcaa ctagctccat ttacagccat ttctaaaatg gcagcttcag
     4921 ttctagagaa gaaagaacaa catcagcagt aaagtccatg gaatagctag tggtctgtgt
     4981 ttcttttcgc cattgcctag cttgccgtaa tgattctata atgccatcat gcagcaatta
     5041 tgagaggcta ggtcatccaa agagaagacc ctatcaatgt aggttgcaaa atctaacccc
     5101 taaggaagtg cagtctttga tttgatttcc ctagtaacct tgcagatatg tttaaccaag
     5161 ccatagccca tgccttttga gggctgaaca aataagggac ttactgataa tttacttttg
     5221 atcacattaa ggtgttctca ccttgaaatc ttatacactg aaatggccat tgatttaggc
     5281 cactggctta gagtactcct tcccctgcat gacactgatt acaaatactt tcctattcat
     5341 actttccaat tatgagatgg actgtgggta ctgggagtga tcactaacac catagtaatg
     5401 tctaatattc acaggcagat ctgcttgggg aagctagtta tgtgaaaggc aaatagagtc
     5461 atacagtagc tcaaaaggca accataattc tctttggtgc aggtcttggg agcgtgatct
     5521 agattacact gcaccattcc caagttaatc ccctgaaaac ttactctcaa ctggagcaaa
     5581 tgaactttgg tcccaaatat ccatcttttc agtagcgtta attatgctct gtttccaact
     5641 gcatttcctt tccaattgaa ttaaagtgtg gcctcgtttt tagtcattta aaattgtttt
     5701 ctaagtaatt gctgcctcta ttatggcact tcaattttgc actgtctttt gagattcaag
     5761 aaaaatttct attctttttt ttgcatccaa ttgtgcctga acttttaaaa tatgtaaatg
     5821 ctgccatgtt ccaaacccat cgtcagtgtg tgtgtttaga gctgtgcacc ctagaaacaa
     5881 catattgtcc catgagcagg tgcctgagac acagacccct ttgcattcac agagaggtca
     5941 ttggttatag agacttgaat taataagtga cattatgcca gtttctgttc tctcacaggt
     6001 gataaacaat gctttttgtg cactacatac tcttcagtgt agagctcttg ttttatggga
     6061 aaaggctcaa atgccaaatt gtgtttgatg gattaatatg cccttttgcc gatgcatact
     6121 attactgatg tgactcggtt ttgtcgcagc tttgctttgt ttaatgaaac acacttgtaa
     6181 acctcttttg cactttgaaa aagaatccag cgggatgctc gagcacctgt aaacaatttt
     6241 ctcaacctat ttgatgttca aataaagaat taaactaaa
//
