LOCUS       NM_012291               6618 bp    mRNA    linear   PRI 09-JUL-2024
DEFINITION  Homo sapiens extra spindle pole bodies like 1, separase (ESPL1),
            mRNA.
ACCESSION   NM_012291
VERSION     NM_012291.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Yang,Y., Sheng,Y., Zheng,J., Ma,A., Chen,S., Lin,J., Yang,X.,
            Liang,Y., Zhang,Y. and Zheng,X.
  TITLE     Upregulation of ESPL1 is associated with poor prognostic outcomes
            in endometrial cancer
  JOURNAL   Biomarkers 29 (4), 185-193 (2024)
   PUBMED   38568742
  REMARK    GeneRIF: Upregulation of ESPL1 is associated with poor prognostic
            outcomes in endometrial cancer.
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Yu,J., Morgan,D.O. and Boland,A.
  TITLE     The molecular mechanisms of human separase regulation
  JOURNAL   Biochem Soc Trans 51 (3), 1225-1233 (2023)
   PUBMED   37140261
  REMARK    GeneRIF: The molecular mechanisms of human separase regulation.
            Review article
REFERENCE   3  (bases 1 to 6618)
  AUTHORS   Yu,J., Raia,P., Ghent,C.M., Raisch,T., Sadian,Y., Cavadini,S.,
            Sabale,P.M., Barford,D., Raunser,S., Morgan,D.O. and Boland,A.
  TITLE     Structural basis of human separase regulation by securin and
            CDK1-cyclin B1
  JOURNAL   Nature 596 (7870), 138-142 (2021)
   PUBMED   34290405
  REMARK    GeneRIF: Structural basis of human separase regulation by securin
            and CDK1-cyclin B1.
REFERENCE   4  (bases 1 to 6618)
  AUTHORS   Aouadi,E., Fornier,M., Gosseye,A., Castillo-Ferrer,C. and
            Frachet,V.
  TITLE     [Separase, a key-player of mitosis: A new target for cancer
            therapy?]
  JOURNAL   Med Sci (Paris) 37 (6-7), 684-686 (2021)
   PUBMED   34180834
  REMARK    GeneRIF: [Separase, a key-player of mitosis: A new target for
            cancer therapy?]', trans 'La separase, proteine-cle de la mitose -
            Une nouvelle cible therapeutique anti-cancereuse ?
REFERENCE   5  (bases 1 to 6618)
  AUTHORS   Wang,R., Zang,W., Hu,B., Deng,D., Ling,X., Zhou,H., Su,M. and
            Jiang,J.
  TITLE     Serum ESPL1 Can Be Used as a Biomarker for Patients With Hepatitis
            B Virus-Related Liver Cancer: A Chinese Case-Control Study
  JOURNAL   Technol Cancer Res Treat 19, 1533033820980785 (2020)
   PUBMED   33308056
  REMARK    GeneRIF: Serum ESPL1 Can Be Used as a Biomarker for Patients With
            Hepatitis B Virus-Related Liver Cancer: A Chinese Case-Control
            Study.
REFERENCE   6  (bases 1 to 6618)
  AUTHORS   Waizenegger,I., Gimenez-Abian,J.F., Wernic,D. and Peters,J.M.
  TITLE     Regulation of human separase by securin binding and autocleavage
  JOURNAL   Curr Biol 12 (16), 1368-1378 (2002)
   PUBMED   12194817
  REMARK    GeneRIF: Data suggest that separase is required for sister
            chromatid separation during mitosis in human cells, and that
            securin inhibits separase by blocking the access of substrates to
            the active site of separase.
REFERENCE   7  (bases 1 to 6618)
  AUTHORS   Chen,F., Kamradt,M., Mulcahy,M., Byun,Y., Xu,H., McKay,M.J. and
            Cryns,V.L.
  TITLE     Caspase proteolysis of the cohesin component RAD21 promotes
            apoptosis
  JOURNAL   J Biol Chem 277 (19), 16775-16781 (2002)
   PUBMED   11875078
REFERENCE   8  (bases 1 to 6618)
  AUTHORS   Stemmann,O., Zou,H., Gerber,S.A., Gygi,S.P. and Kirschner,M.W.
  TITLE     Dual inhibition of sister chromatid separation at metaphase
  JOURNAL   Cell 107 (6), 715-726 (2001)
   PUBMED   11747808
REFERENCE   9  (bases 1 to 6618)
  AUTHORS   Hauf,S., Waizenegger,I.C. and Peters,J.M.
  TITLE     Cohesin cleavage by separase required for anaphase and cytokinesis
            in human cells
  JOURNAL   Science 293 (5533), 1320-1323 (2001)
   PUBMED   11509732
REFERENCE   10 (bases 1 to 6618)
  AUTHORS   Zou,H., McGarry,T.J., Bernal,T. and Kirschner,M.W.
  TITLE     Identification of a vertebrate sister-chromatid separation
            inhibitor involved in transformation and tumorigenesis
  JOURNAL   Science 285 (5426), 418-422 (1999)
   PUBMED   10411507
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC021072.27 and AC073611.29.
            
            On Aug 1, 2019 this sequence version replaced NM_012291.4.
            
            Summary: Stable cohesion between sister chromatids before anaphase
            and their timely separation during anaphase are critical for
            chromosome inheritance. In vertebrates, sister chromatid cohesion
            is released in 2 steps via distinct mechanisms. The first step
            involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM
            300826) in the cohesin complex. The second step involves cleavage
            of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or
            separase, which initiates the final separation of sister chromatids
            (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853560.6913.1,
                                           SRR11853559.7861.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000257934.9/ ENSP00000257934.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-79                AC021072.27        7864-7942           c
            80-172              AC021072.27        7394-7486           c
            173-1234            AC021072.27        6156-7217           c
            1235-1339           AC021072.27        5759-5863           c
            1340-1460           AC021072.27        5443-5563           c
            1461-1597           AC021072.27        3384-3520           c
            1598-1791           AC021072.27        1231-1424           c
            1792-2031           AC073611.29        2384-2623
            2032-2176           AC073611.29        2847-2991
            2177-2315           AC073611.29        3234-3372
            2316-2455           AC073611.29        3585-3724
            2456-2590           AC073611.29        5496-5630
            2591-2710           AC073611.29        7271-7390
            2711-2882           AC073611.29        8028-8199
            2883-3011           AC073611.29        8893-9021
            3012-3168           AC073611.29        9146-9302
            3169-3278           AC073611.29        9822-9931
            3279-4267           AC073611.29        11688-12676
            4268-4637           AC073611.29        13736-14105
            4638-4799           AC073611.29        14302-14463
            4800-5013           AC073611.29        14854-15067
            5014-5204           AC073611.29        15168-15358
            5205-5332           AC073611.29        15849-15976
            5333-5455           AC073611.29        16111-16233
            5456-5611           AC073611.29        16605-16760
            5612-5782           AC073611.29        17454-17624
            5783-5887           AC073611.29        17748-17852
            5888-6003           AC073611.29        18042-18157
            6004-6087           AC073611.29        18338-18421
            6088-6252           AC073611.29        18570-18734
            6253-6618           AC073611.29        19037-19402
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.13"
     gene            1..6618
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /note="extra spindle pole bodies like 1, separase"
                     /db_xref="GeneID:9700"
                     /db_xref="HGNC:HGNC:16856"
                     /db_xref="MIM:604143"
     exon            1..79
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            80..172
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     CDS             92..6454
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /EC_number="3.4.22.49"
                     /note="caspase-like protein ESPL1; extra spindle
                     poles-like 1 protein; extra spindle poles like 1; separin,
                     cysteine protease; extra spindle pole bodies homolog 1;
                     extra spindle pole bodies 1, separase"
                     /codon_start=1
                     /product="separin"
                     /protein_id="NP_036423.4"
                     /db_xref="CCDS:CCDS8852.1"
                     /db_xref="GeneID:9700"
                     /db_xref="HGNC:HGNC:16856"
                     /db_xref="MIM:604143"
                     /translation="MRSFKRVNFGTLLSSQKEAEELLPALKEFLSNPPAGFPSSRSDA
                     ERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILF
                     VLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLE
                     RRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADAD
                     FLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVE
                     KAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLR
                     ALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQS
                     FLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELT
                     DHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFR
                     LQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQL
                     KTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWA
                     RATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALL
                     WLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAAD
                     AAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLL
                     LRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLL
                     SLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSL
                     PSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLD
                     YGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALF
                     LVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQV
                     PCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTC
                     DCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQA
                     SLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLE
                     PWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGR
                     QKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVP
                     QAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKT
                     DAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELT
                     DNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKKLPSPCPDKES
                     DKDLGPRLRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGH
                     RDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQEMPGDV
                     PLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLL
                     LTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALD
                     HRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIM
                     LSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQ
                     KLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNL
                     SSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVL
                     RLSCRAVALLFGCSSAALAVRGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTE
                     ALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR"
     exon            173..1234
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1339
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1460
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1461..1597
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1598..1791
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..2031
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            2032..2176
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            2177..2315
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            2316..2455
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            2456..2590
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            2591..2710
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            2711..2882
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            2883..3011
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            3012..3168
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            3169..3278
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            3279..4267
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            4268..4637
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            4638..4799
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            4800..5013
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            5014..5204
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            5205..5332
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            5333..5455
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            5456..5611
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            5612..5782
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            5783..5887
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            5888..6003
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            6004..6087
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            6088..6252
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            6253..6618
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6598..6603
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /note="hexamer: AATAAA"
     polyA_site      6618
                     /gene="ESPL1"
                     /gene_synonym="ESP1; SEPA"
                     /note="major polyA site"
ORIGIN      
        1 ggttacattt tggatcctcg cggagtactg gtcaggcggt taagtcctgt acctaggaaa
       61 gagggcgagc tctggggcgc tctccggtgt catgaggagc ttcaaaagag tcaactttgg
      121 gactctgcta agcagccaga aggaggctga agagttgctg cccgccttga aggagttcct
      181 gtccaaccct ccagctggtt ttcccagcag ccgatctgat gctgagagga gacaagcttg
      241 tgatgccatc ctgagggctt gcaaccagca gctgactgct aagctagctt gccctaggca
      301 tctggggagc ctgctggagc tggcagagct ggcctgtgat ggctacttag tgtctacccc
      361 acagcgtcct cccctctacc tggaacgaat tctctttgtc ttactgcgga atgctgctgc
      421 acaaggaagc ccagaggcca cactccgcct tgctcagccc ctccatgcct gcttggtgca
      481 gtgctctcgc gaggctgctc cccaggacta tgaggccgtg gctcggggca gcttttctct
      541 gctttggaag ggggcagaag ccctgttgga acggcgagct gcatttgcag ctcggctgaa
      601 ggccttgagc ttcctagtac tcttggagga tgaaagtacc ccttgtgagg ttcctcactt
      661 tgcttctcca acagcctgtc gagcggtagc tgcccatcag ctatttgatg ccagtggcca
      721 tggtctaaat gaagcagatg ctgatttcct agatgacctg ctctccaggc acgtgatcag
      781 agccttggtg ggtgagagag ggagctcttc tgggcttctt tctccccaga gggccctctg
      841 cctcttggag ctcaccttgg aacactgccg tcgcttttgc tggagccgcc accatgacaa
      901 agccatcagc gcagtggaga aggctcacag ttacctaagg aacaccaatc tagcccctag
      961 ccttcagcta tgtcagctgg gggttaagct gctgcaggtt ggggaggaag gacctcaggc
     1021 agtggccaag cttctgatca aggcatcagc tgtcctgagc aagagtatgg aggcaccatc
     1081 acccccactt cgggcattgt atgagagctg ccagttcttc ctttcaggcc tggaacgagg
     1141 caccaagagg cgctatagac ttgatgccat tctgagcctc tttgcttttc ttggagggta
     1201 ctgctctctt ctgcagcagc tgcgggatga tggtgtgtat gggggctcct ccaagcaaca
     1261 gcagtctttt cttcagatgt actttcaggg acttcacctc tacactgtgg tggtttatga
     1321 ctttgcccaa ggctgtcaga tagttgattt ggctgacctg acccaactag tggacagttg
     1381 taaatctacc gttgtctgga tgctggaggc cttagagggc ctgtcgggcc aagagctgac
     1441 ggaccacatg gggatgaccg cttcttacac cagtaatttg gcctacagct tctatagtca
     1501 caagctctat gccgaggcct gtgccatctc tgagccgctc tgtcagcacc tgggtttggt
     1561 gaagccaggc acttatcccg aggtgcctcc tgagaagttg cacaggtgct tccggctaca
     1621 agtagagagt ttgaagaaac tgggtaaaca ggcccagggc tgcaagatgg tgattttgtg
     1681 gctggcagcc ctgcaaccct gtagccctga acacatggct gagccagtca ctttctgggt
     1741 tcgggtcaag atggatgcgg ccagggctgg agacaaggag ctacagctaa agactctgcg
     1801 agacagcctc agtggctggg acccggagac cctggccctc ctgctgaggg aggagctgca
     1861 ggcctacaag gcggtgcggg ccgacactgg acaggaacgc ttcaacatca tctgtgacct
     1921 cctggagctg agccccgagg agacaccagc cggggcctgg gcacgagcca cccacctggt
     1981 agaactggct caggtgctct gctaccacga ctttacgcag cagaccaact gctctgctct
     2041 ggatgctatc cgggaagccc tgcagcttct ggactctgtg aggcctgagg cccaggccag
     2101 agatcagctt ctggacgata aagcacaggc cttgctgtgg ctttacatct gtactctgga
     2161 agccaaaatg caggaaggta tcgagcggga tcggagagcc caggcccctg gtaacttgga
     2221 ggaatttgaa gtcaatgacc tgaactatga agataaactc caggaagatc gtttcctata
     2281 cagtaacatt gccttcaacc tggctgcaga tgctgctcag tccaaatgcc tggaccaagc
     2341 cctggccctg tggaaggagc tgcttacaaa ggggcaggcc ccagctgtac ggtgtctcca
     2401 gcagacagca gcctcactgc agatcctagc agccctctac cagctggtgg caaagcccat
     2461 gcaggctctg gaggtcctcc tgctgctacg gattgtctct gagagactga aggaccactc
     2521 gaaggcagct ggctcctcct gccacatcac ccagctcctc ctgaccctcg gctgtcccag
     2581 ctatgcccag ttacacctgg aagaggcagc atcgagcctg aagcatctcg atcagactac
     2641 tgacacatac ctgctccttt ccctgacctg tgatctgctt cgaagtcaac tctactggac
     2701 tcaccagaag gtgaccaagg gtgtctctct gctgctgtct gtgcttcggg atcctgccct
     2761 ccagaagtcc tccaaggctt ggtacttgct gcgtgtccag gtcctgcagc tggtggcagc
     2821 ttaccttagc ctcccgtcaa acaacctctc acactccctg tgggagcagc tctgtgccca
     2881 aggctggcag acacctgaga tagctctcat agactcccat aagctcctcc gaagcatcat
     2941 cctcctgctg atgggcagtg acattctctc aactcagaaa gcagctgtgg agacatcgtt
     3001 tttggactat ggtgaaaatc tggtacaaaa atggcaggtt ctttcagagg tgctgagctg
     3061 ctcagagaag ctggtctgcc acctgggccg cctgggtagt gtgagtgaag ccaaggcctt
     3121 ttgcttggag gccctaaaac ttacaacaaa gctgcagata ccacgccagt gtgccctgtt
     3181 cctggtgctg aagggcgagc tggagctggc ccgcaatgac attgatctct gtcagtcgga
     3241 cctgcagcag gttctgttct tgcttgagtc ttgcacagag tttggtgggg tgactcagca
     3301 cctggactct gtgaagaagg tccacctgca gaaggggaag cagcaggccc aggtcccctg
     3361 tcctccacag ctcccagagg aggagctctt cctaagaggc cctgctctag agctggtggc
     3421 cactgtggcc aaggagcctg gccccatagc accttctaca aactcctccc cagtcttgaa
     3481 aaccaagccc cagcccatac ccaacttcct gtcccattca cccacctgtg actgctcgct
     3541 ctgcgccagc cctgtcctca cagcagtctg tctgcgctgg gtattggtca cggcaggggt
     3601 gaggctggcc atgggccacc aagcccaggg tctggatctg ctgcaggtcg tgctgaaggg
     3661 ctgtcctgaa gccgctgagc gcctcaccca agctctccaa gcttccctga atcataaaac
     3721 acccccctcc ttggttccaa gcctcttgga tgagatcttg gctcaagcat acacactgtt
     3781 ggcactggag ggcctgaacc agccatcaaa cgagagcctg cagaaggttc tacagtcagg
     3841 gctgaagttt gtagcagcac ggatacccca cctagagccc tggcgagcca gcctgctctt
     3901 gatttgggcc ctcacaaaac taggtggcct cagctgctgt actacccaac tttttgcaag
     3961 ctcctggggc tggcagccac cattaataaa aagtgtccct ggctcagagc cctctaagac
     4021 tcagggccaa aaacgttctg gacgagggcg ccaaaagtta gcctctgctc ccctgcgcct
     4081 caataatacc tctcagaaag gtctggaagg tagaggactg ccctgcacac ctaaaccccc
     4141 agaccggatc aggcaagctg gccctcatgt ccccttcacg gtgtttgagg aagtctgccc
     4201 tacagagagc aagcctgaag taccccaggc ccccagggta caacagagag tccagacgcg
     4261 cctcaaggtg aacttcagtg atgacagtga cttggaagac cctgtctcag ctgaggcctg
     4321 gctggcagag gagcctaaga gacggggcac tgcttcccgg ggccgggggc gagcaaggaa
     4381 gggcctgagc ctaaagacgg atgccgtggt tgccccaggt agtgcccctg ggaaccctgg
     4441 cctgaatggc aggagccgga gggccaagaa ggtggcatca agacattgtg aggagcggcg
     4501 tccccagagg gccagtgacc aggccaggcc tggccctgag atcatgagga ccatccctga
     4561 ggaagaactg actgacaact ggagaaaaat gagctttgag atcctcaggg gctctgacgg
     4621 ggaagactca gcctcaggtg ggaagactcc agctccgggc cctgaggcag cttctggaga
     4681 atgggagctg ctgaggctgg attccagcaa gaagaagctg cccagcccat gcccagacaa
     4741 ggagagtgac aaggaccttg gtcctcggct ccggctcccc tcagcccccg tagccactgg
     4801 tctttctacc ctggactcca tctgtgactc cctgagtgtt gctttccggg gcattagtca
     4861 ctgtcctcct agtgggctct atgcccacct ctgccgcttc ctggccttgt gcctgggcca
     4921 ccgggatcct tatgccactg ctttccttgt caccgagtct gtctccatca cctgtcgcca
     4981 ccagctgctc acccacctcc acagacagct cagcaaggcc cagaagcacc gaggatcact
     5041 tgaaatagca gaccagctgc aggggctgag ccttcaggag atgcctggag atgtccccct
     5101 ggcccgcatc cagcgcctct tttccttcag ggctttggaa tctggccact tcccccagcc
     5161 tgaaaaggag agtttccagg agcgcctggc tctgatcccc agtggggtga ctgtgtgtgt
     5221 gttggccctg gccaccctcc agcccggaac cgtgggcaac accctcctgc tgacccggct
     5281 ggaaaaggac agtcccccag tcagtgtgca gattcccact ggccagaaca agcttcatct
     5341 gcgttcagtc ctgaatgagt ttgatgccat ccagaaggca cagaaagaga acagcagctg
     5401 tactgacaag cgagaatggt ggacagggcg gctggcactg gaccacagga tggaggttct
     5461 catcgcttcc ctagagaagt ctgtgctggg ctgctggaag gggctgctgc tgccgtccag
     5521 tgaggagccc ggccctgccc aggaggcctc ccgcctacag gagctgctac aggactgtgg
     5581 ctggaaatat cctgaccgca ctctgctgaa aatcatgctc agtggtgccg gtgccctcac
     5641 ccctcaggac attcaggccc tggcctacgg gctgtgccca acccagccag agcgagccca
     5701 ggagctcctg aatgaggcag taggacgtct acagggcctg acagtaccaa gcaatagcca
     5761 ccttgtcttg gtcctagaca aggacttgca gaagctgccg tgggaaagca tgcccagcct
     5821 ccaagcactg cctgtcaccc ggctgccctc cttccgcttc ctactcagct actccatcat
     5881 caaagagtat ggggcctcgc cagtgctgag tcaaggggtg gatccacgaa gtaccttcta
     5941 tgtcctgaac cctcacaata acctgtcaag cacagaggag caatttcgag ccaatttcag
     6001 cagtgaagct ggctggagag gagtggttgg ggaggtgcca agacctgaac aggtgcagga
     6061 agccctgaca aagcatgatt tgtatatcta tgcagggcat ggggctggtg cccgcttcct
     6121 tgatgggcag gctgtcctgc ggctgagctg tcgggcagtg gccctgctgt ttggctgtag
     6181 cagtgcggcc ctggctgtgc gtggaaacct ggagggggct ggcatcgtgc tcaagtacat
     6241 catggctggt tgccccttgt ttctgggtaa tctctgggat gtgactgacc gcgacattga
     6301 ccgctacacg gaagctctgc tgcaaggctg gcttggagca ggcccagggg ccccccttct
     6361 ctactatgta aaccaggccc gccaagctcc ccgactcaag tatcttattg gggctgcacc
     6421 tatagcctat ggcttgcctg tctctctgcg gtaaccccat ggagctgtct tattgatgct
     6481 agaagcctca taactgttct acctccaagg ttagatttaa tccttaggat aactctttta
     6541 aagtgatttt ccccagtgtt ttatatgaaa catttccttt tgatttaacc tcagtataat
     6601 aaagatacat catttaaa
//
