LOCUS       NM_001433               7895 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1),
            mRNA.
ACCESSION   NM_001433
VERSION     NM_001433.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Jin,H., Yeom,J.H., Shin,E., Ha,Y., Liu,H., Kim,D., Joo,M.,
            Kim,Y.H., Kim,H.K., Ryu,M., Kim,H.M., Kim,J., Kim,K.P., Hahn,Y.,
            Bae,J. and Lee,K.
  TITLE     5'-tRNAGly(GCC) halves generated by IRE1alpha are linked to the ER
            stress response
  JOURNAL   Nat Commun 15 (1), 9273 (2024)
   PUBMED   39468069
  REMARK    GeneRIF: 5'-tRNA[Gly(GCC)] halves generated by IRE1alpha are linked
            to the ER stress response.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  AUTHORS   Kettel,P., Marosits,L., Spinetti,E., Rechberger,M., Giannini,C.,
            Radler,P., Niedermoser,I., Fischer,I., Versteeg,G.A., Loose,M.,
            Covino,R. and Karagoz,G.E.
  TITLE     Disordered regions in the IRE1alpha ER lumenal domain mediate its
            stress-induced clustering
  JOURNAL   EMBO J 43 (20), 4668-4698 (2024)
   PUBMED   39232130
  REMARK    GeneRIF: Disordered regions in the IRE1alpha ER lumenal domain
            mediate its stress-induced clustering.
REFERENCE   3  (bases 1 to 7895)
  AUTHORS   Mou,L., Sun,D., Qu,J., Tan,X., Wang,S., Zeng,Q. and Liu,C.
  TITLE     GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP
            facilitates iodoacetic acid-mediated testosterone decline
  JOURNAL   J Hazard Mater 476, 135101 (2024)
   PUBMED   39002476
  REMARK    GeneRIF: GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP
            facilitates iodoacetic acid-mediated testosterone decline.
REFERENCE   4  (bases 1 to 7895)
  AUTHORS   Minchenko,O.H., Sliusar,M.Y., Khikhlo,Y.P., Halkin,O.V.,
            Viletska,Y.M., Khita,O.O. and Minchenko,D.O.
  TITLE     Knockdown of ERN1 disturbs the expression of phosphoserine
            aminotransferase 1 and related genes in glioblastoma cells
  JOURNAL   Arch Biochem Biophys 759, 110104 (2024)
   PUBMED   39059599
  REMARK    GeneRIF: Knockdown of ERN1 disturbs the expression of phosphoserine
            aminotransferase 1 and related genes in glioblastoma cells.
REFERENCE   5  (bases 1 to 7895)
  AUTHORS   Yoshida,H., Matsui,T., Yamamoto,A., Okada,T. and Mori,K.
  TITLE     XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER
            stress to produce a highly active transcription factor
  JOURNAL   Cell 107 (7), 881-891 (2001)
   PUBMED   11779464
REFERENCE   6  (bases 1 to 7895)
  AUTHORS   Yoneda,T., Imaizumi,K., Oono,K., Yui,D., Gomi,F., Katayama,T. and
            Tohyama,M.
  TITLE     Activation of caspase-12, an endoplastic reticulum (ER) resident
            caspase, through tumor necrosis factor receptor-associated factor
            2-dependent mechanism in response to the ER stress
  JOURNAL   J Biol Chem 276 (17), 13935-13940 (2001)
   PUBMED   11278723
REFERENCE   7  (bases 1 to 7895)
  AUTHORS   Iwawaki,T., Hosoda,A., Okuda,T., Kamigori,Y., Nomura-Furuwatari,C.,
            Kimata,Y., Tsuru,A. and Kohno,K.
  TITLE     Translational control by the ER transmembrane kinase/ribonuclease
            IRE1 under ER stress
  JOURNAL   Nat Cell Biol 3 (2), 158-164 (2001)
   PUBMED   11175748
REFERENCE   8  (bases 1 to 7895)
  AUTHORS   Urano,F., Wang,X., Bertolotti,A., Zhang,Y., Chung,P., Harding,H.P.
            and Ron,D.
  TITLE     Coupling of stress in the ER to activation of JNK protein kinases
            by transmembrane protein kinase IRE1
  JOURNAL   Science 287 (5453), 664-666 (2000)
   PUBMED   10650002
REFERENCE   9  (bases 1 to 7895)
  AUTHORS   Katayama,T., Imaizumi,K., Sato,N., Miyoshi,K., Kudo,T., Hitomi,J.,
            Morihara,T., Yoneda,T., Gomi,F., Mori,Y., Nakano,Y., Takeda,J.,
            Tsuda,T., Itoyama,Y., Murayama,O., Takashima,A., St
            George-Hyslop,P., Takeda,M. and Tohyama,M.
  TITLE     Presenilin-1 mutations downregulate the signalling pathway of the
            unfolded-protein response
  JOURNAL   Nat Cell Biol 1 (8), 479-485 (1999)
   PUBMED   10587643
REFERENCE   10 (bases 1 to 7895)
  AUTHORS   Tirasophon,W., Welihinda,A.A. and Kaufman,R.J.
  TITLE     A stress response pathway from the endoplasmic reticulum to the
            nucleus requires a novel bifunctional protein
            kinase/endoribonuclease (Ire1p) in mammalian cells
  JOURNAL   Genes Dev 12 (12), 1812-1824 (1998)
   PUBMED   9637683
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC025362.12, DB128514.1,
            AB209869.1, AC005803.1, BC130405.1, BC070350.1 and CA311378.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001433.4.
            
            Summary: This gene encodes the transmembrane protein kinase
            inositol-requiring enzyme 1. The encoded protein contains two
            functional catalytic domains, a serine/threonine-protein kinase
            domain and an endoribonuclease domain. This protein functions as a
            sensor of unfolded proteins in the endoplasmic reticulum (ER) and
            triggers an intracellular signaling pathway termed the unfolded
            protein response (UPR). The UPR is an ER stress response that is
            conserved from yeast to mammals and activates genes involved in
            degrading misfolded proteins, regulating protein synthesis and
            activating molecular chaperones. This protein specifically mediates
            the splicing and activation of the stress response transcription
            factor X-box binding protein 1. [provided by RefSeq, Aug 2017].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.231264.1, AB209869.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000433197.4/ ENSP00000401445.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AC025362.12        88654-88655         c
            3-19                DB128514.1         1-17
            20-1092             AB209869.1         1-1073
            1093-1202           AC005803.1         144670-144779       c
            1203-2589           AB209869.1         1184-2570
            2590-2644           AC005803.1         128542-128596       c
            2645-3504           BC130405.1         2576-3435
            3505-7135           AC005803.1         120586-124216       c
            7136-7789           BC070350.1         663-1316
            7790-7895           CA311378.1         18-123              c
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q23.3"
     gene            1..7895
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /note="endoplasmic reticulum to nucleus signaling 1"
                     /db_xref="GeneID:2081"
                     /db_xref="HGNC:HGNC:3449"
                     /db_xref="MIM:604033"
     exon            1..169
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..3049
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /EC_number="2.7.11.1"
                     /note="ER to nucleus signalling 1; inositol-requiring 1;
                     protein kinase/endoribonuclease; serine/threonine-protein
                     kinase/endoribonuclease IRE1; ire1-alpha;
                     inositol-requiring protein 1; inositol-requiring enzyme 1"
                     /codon_start=1
                     /product="serine/threonine-protein kinase/endoribonuclease
                     IRE1 precursor"
                     /protein_id="NP_001424.3"
                     /db_xref="CCDS:CCDS45762.1"
                     /db_xref="GeneID:2081"
                     /db_xref="HGNC:HGNC:3449"
                     /db_xref="MIM:604033"
                     /translation="MPARRLLLLLTLLLPGLGIFGSTSTVTLPETLLFVSTLDGSLHA
                     VSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGSKNNEGLTKLPFTIPE
                     LVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTE
                     YTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDVLW
                     IQNYASPVVAFYVWQREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEA
                     KSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVI
                     TPSTDVKFDPGLKSKNKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIP
                     ADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILS
                     TFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDG
                     ELLDTSGPYSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEETSVVIV
                     GKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPN
                     VIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSL
                     NIVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAP
                     EMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQANILLGACSLDCLHPE
                     KHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKESLDG
                     PIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYREL
                     PAEVRETLGSLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQPPV
                     TPDAL"
     exon            170..290
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            291..324
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            325..397
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            398..470
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            471..593
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            594..695
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            696..957
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1036
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1202
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..1321
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1513
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            1514..1787
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            1788..1878
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..2068
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            2069..2168
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            2169..2368
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            2369..2516
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            2517..2644
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            2645..2768
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            2769..2836
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     exon            2837..7895
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7871..7876
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /note="hexamer: AATAAA"
     polyA_site      7895
                     /gene="ERN1"
                     /gene_synonym="hIRE1p; IRE1; IRE1a; IRE1P"
                     /note="major polyA site"
ORIGIN      
        1 gctgcctagt cagttctgcg tccgctgagg ctcggtcacc gcctcgctgt cgtcgcggcg
       61 cccccgcccc gtcctctgtc cgtaccgccc ccggagccag ggccgagtcc tcgccatgcc
      121 ggcccggcgg ctgctgctgc tgctgacgct gctgctgccc ggcctcggga tttttggaag
      181 taccagcaca gtgacgcttc ctgaaacctt gttgtttgtg tcaacgctgg atggaagttt
      241 gcatgctgtc agcaagagga caggctcaat caaatggact ttaaaagaag atccagtcct
      301 gcaggtccca acacatgtgg aagagcctgc ctttctccca gatcctaatg atggcagcct
      361 gtatacgctt ggaagcaaga ataatgaagg cctgacgaaa cttcctttta ccatcccaga
      421 attggtgcag gcatccccat gccgaagttc agatggaatc ctctacatgg gtaaaaagca
      481 ggacatctgg tatgttattg acctcctgac cggagagaag cagcagactt tgtcatcggc
      541 ctttgcagat agtctctgcc catcaacctc tcttctgtat cttgggcgaa cagaatacac
      601 catcaccatg tacgacacca aaacccgaga gctccggtgg aatgccacct actttgacta
      661 tgcggcctca ctgcctgagg acgacgtgga ctacaagatg tcccactttg tgtccaatgg
      721 tgatgggctg gtggtgactg tggacagtga atctggggac gtcctgtgga tccaaaacta
      781 cgcctcccct gtggtggcct tttatgtctg gcagcgggag ggtctgagga aggtgatgca
      841 catcaatgtc gctgtggaga ccctgcgcta tctgaccttc atgtctgggg aggtggggcg
      901 catcacaaag tggaagtacc cgttccccaa ggagacagag gccaagagca agctgacgcc
      961 cactctgtat gttgggaaat actctaccag cctctatgcc tctccctcaa tggtacacga
     1021 gggggttgct gtcgtgcccc gcggcagcac acttcctttg ctggaagggc cccagactga
     1081 tggcgtcacc attggggaca agggggagtg tgtgatcacg cccagcacgg acgtcaagtt
     1141 tgatcccgga ctcaaaagca agaacaagct caactacttg aggaattact ggcttctgat
     1201 aggacaccat gaaaccccac tgtctgcgtc taccaagatg ctggagagat ttcccaacaa
     1261 tctacccaaa catcgggaaa atgtgattcc tgctgattca gagaaaaaga gctttgagga
     1321 agttatcaac ctggttgacc agacttcaga aaacgcacct accaccgtgt ctcgggatgt
     1381 ggaggagaag cccgcccatg cccctgcccg gcccgaggcc cccgtggact ccatgcttaa
     1441 ggacatggct accatcatcc tgagcacctt cctgctgatt ggctgggtgg ccttcatcat
     1501 cacctatccc ctgagcatgc atcagcagca gcagctccag caccagcagt tccagaagga
     1561 actggagaag atccagctcc tgcagcagca gcagcagcag ctgcccttcc acccacctgg
     1621 agacacggct caggacggcg agctcctgga cacgtctggc ccgtactcag agagctcggg
     1681 caccagcagc cccagcacgt cccccagggc ctccaaccac tcgctctgct ccggcagctc
     1741 tgcctccaag gctggcagca gcccctccct ggaacaagac gatggagatg aggaaaccag
     1801 cgtggtgata gttgggaaaa tttccttctg tcccaaggat gtcctgggcc atggagctga
     1861 gggcacaatt gtgtaccggg gcatgtttga caaccgcgac gtggccgtga agaggatcct
     1921 ccccgagtgt tttagcttcg cagaccgtga ggtccagctg ttgcgagaat cggatgagca
     1981 cccgaacgtg atccgctact tctgcacgga gaaggaccgg caattccagt acattgccat
     2041 cgagctgtgt gcagccaccc tgcaagagta tgtggagcag aaggactttg cgcatctcgg
     2101 cctggagccc atcaccttgc tgcagcagac cacctcgggc ctggcccacc tccactccct
     2161 caacatcgtt cacagagacc taaagccaca caacatcctc atatccatgc ccaatgcaca
     2221 cggcaagatc aaggccatga tctccgactt tggcctctgc aagaagctgg cagtgggcag
     2281 acacagtttc agccgccgat ctggggtgcc tggcacagaa ggctggatcg ctccagagat
     2341 gctgagcgaa gactgtaagg agaaccctac ctacacggtg gacatctttt ctgcaggctg
     2401 cgtcttttac tacgtaatct ctgagggcag ccaccctttt ggcaagtccc tgcagcggca
     2461 ggccaacatc ctcctgggtg cctgcagcct tgactgcttg cacccagaga agcacgaaga
     2521 cgtcattgca cgtgaattga tagagaagat gattgcgatg gatcctcaga aacgcccctc
     2581 agcgaagcat gtgctcaaac acccgttctt ctggagccta gagaagcagc tccagttctt
     2641 ccaggacgtg agcgacagaa tagaaaagga atccctggat ggcccgatcg tgaagcagtt
     2701 agagagaggc gggagagccg tggtgaagat ggactggcgg gagaacatca ctgtccccct
     2761 ccagacagac ctgcgtaaat tcaggaccta taaaggtggt tctgtcagag atctcctccg
     2821 agccatgaga aataagaagc accactaccg ggagctgcct gcagaggtgc gggagacgct
     2881 ggggtccctc cccgacgact tcgtgtgcta cttcacatct cgcttccccc acctcctcgc
     2941 acacacctac cgggccatgg agctgtgcag ccacgagaga ctcttccagc cctactactt
     3001 ccacgagccc ccagagcccc agcccccagt gactccagac gccctctgag cgagggcggc
     3061 ccctctgttc tggtggcccc agctgtgact gagggcctgg tcaccacaat tagagcttga
     3121 tgcctcccgg ctttgcaggg agaccaggct tcccaaacca agtgccttga gctgcctgct
     3181 ctgcagccca cagaggacag tgctgacccc aggaagtggg agaagtggcc cctcgtgacc
     3241 tacagggaac tgggaagatg ctggccccaa aagccttacg gtcatgatgt ctgcaaagga
     3301 gggcctcaga gacagcgcga gtagcacccc cagccatcta ctggataaac ttgcttcaga
     3361 ctttttaaat tcctgcttaa tgtcagtcta caggcctttc aggaagggag aggagggaat
     3421 cgtacatttt gcttgcgtgc tgggacagct aggctgagat gcaccaagta cagccttcac
     3481 tggagaccgg aattgagagg tgggggatgc tgaggagggg gaggacggag ttcagagggt
     3541 gtcgtcctgc agtgtgagat ttctcattga tcacagatgt gcccagagta gcccaggtca
     3601 ctgttaacta gtgtttctgc agaggcagca ggagccatga gcatgaggtg tggcattagg
     3661 gactggtcag ctatgcatgc tggcaggtgg ggttgtgtct gcaggtctca gaaatgaaga
     3721 ggctgctctg ttctggaggc agccgtggcc cagtgccagt ggccagaaca gtggcctttg
     3781 gtgggtgtgt cccgggccat ctcggggtgg tgctcaggag cgcctggggc aagaggtaaa
     3841 gagttccctg gccttcaagg agagcagcga agacccagac aggggccagc cttcaggacc
     3901 agagggaggc cgccgaatgg gaccctcctg gtcaccagga gaaagccctg ggccagcgag
     3961 taggcagtca aactccttcg tccccaaggc cggtggaaca agaggctcgt ggtgagtcag
     4021 ggccagggtg ggtggccaag gccagggtca ccgtgtgctt catgggccag cttttttgtt
     4081 tttcttggca aattttaata actatatttt gattatactg tagaatgcta tgtcagcata
     4141 agtaagctaa acttgaagct ttcttgtgaa gaataaatgc aagatagaat acatcttcta
     4201 ttttttgtgg taccaaaaat caccatcccc tcaagagtgt tcatgtatag aacattctct
     4261 aatgctgaag agtaaaacat tatagcaaca ctatgtaaat gtattgaaca gtatcaaaga
     4321 aatagtctct aaattgtttg taccatattt ttttttctaa acttaacata atttttagct
     4381 ttagtttcag tcaaaacttt gtcttttctc tcccgagagc cttagaggtt aaaatgcaat
     4441 cagcctaccg tgtaaggaga tgttgtccat gtactttctc cagccagttg ggggatcatt
     4501 gcagctcagg cctggtgaac tcagagattc cattcagtat taagaatggg attgttgaat
     4561 tttactcaca gagaaatcac tgtttcttca tgttgtaaga tgttttctgt ttgtgtattt
     4621 gtatcatggt tactcatcaa aagctctcat tctgcctttg tagaattcag ttcccttcct
     4681 ttcatcatag ctaaagtgac ttttttccct actattaacg tgatcctaca tccttaaatc
     4741 tcatcgatta cctcacttag gccttggaac cttggccctt ggtcggtgtc cttggcgtct
     4801 tctaagcaag gctgtgcgtt gttcagaaac gtggccagac cgcatttcct gctgctccca
     4861 tgccgcatgc caggtggcct gagacagagc tccccatacg gctgcaaggt gctttacctg
     4921 tgggctttgg cagtaaccca agagaggatc agaaggtgga gaaggtgcca cgagtgattt
     4981 aacaggcctg ccacagggag tgcccccagc ccagctcgtt ctcagcacag gttttctctt
     5041 tgggagtacc caggtgattt ctagtgaccc aattttgtgt catctccctg ttttagcccc
     5101 acttgcctag agacaactgt ttccgatgcc ttttctgctt atcatactag tttctaacca
     5161 cgcagatttc tcaaaatcat ttattcaatg tattttattt gagcacttag tgtattgagc
     5221 taggcaggat atagggtgcc ggagatacag cgatgaacaa gacaggcaaa acttctgctt
     5281 tcctaaaact tgtgatgaga gagaacaata aaaaagtgtt gctgccacaa agaaaccaaa
     5341 gtgtgtgggg aagggcgcgt gtttgcggtt tacatctctt ctgtcccaga atcacaggga
     5401 tctggtccgg tcacctctgg tctttcctct tagtcgcctt taggagccct ggttcccgtc
     5461 catcctctgg ggggtttgtt tgaagagatc tcgtgtgggt acttgtcatg aaaacacctt
     5521 gggcatcatc tggtgtatcc agttctagtc tcgagaattc tggtttccca ctgtgctcag
     5581 caagtggaaa gttcttttca ggccagaaca gctctgcacc atcacatatc gtgttgcggc
     5641 ttagctgttt ggtctgtagt tcaggttatg ggacttctcc aatcctggaa ggctgttgag
     5701 ctttttagaa gtactgtacg ctatcttcaa gatggagctt ggtcacatct gttaggaatc
     5761 caaaggacac tatgacttat ttaaatcttg tcttactaaa cctctcttgg gcacgtgtgc
     5821 cagaatttct cttgttgctt cttgagtctt tttaatttca gtgttttttc gtttgttttt
     5881 tgtttttttg agacagagtc tcgctctgtc acccaggctg gagtgcagtg gcacgacctc
     5941 agctcactgc aacctccgcc tccttggttc aagcaattct cctgcctcag cctcccgagt
     6001 agctgggatt acaggtgtgt gccaccacgc tcggctaatt ttttatattt tttttagtag
     6061 agacggggtt tccccatgtt aggcaggatg gtctcgatct cctaacctcg tgatccgcct
     6121 gcctcagcct cccaaagtgc tgggattaca ggcttgagcc accgcacccg gcctaatctc
     6181 agtttttgaa gtgctccaca agtcattagg caccaaaaca ttttcacctg gggaacactg
     6241 gcatttccct gattagctgt gaagcaatct agtggctaag tgtgaaatcc tgggtgcgca
     6301 ggtgttctca ctcccgccgt gttctcagtg cagtggtggt cagaggccct tccaaggaga
     6361 catcactctg atcagttaca gatagatgtt ctggaagatc tgcaggtgag tagatccagc
     6421 agagtttctt cccaccaact ctagaagaaa gggccttatc agagttgacc ctgagccttt
     6481 ggtaaggttt tgtgtgcatg cgattcagtt atctttggca attcttttct tgctgcagtg
     6541 agagattaat tggttgctga tcaaaccgtt catgcagatg gggggacctt tggattgtac
     6601 ggctttctcc tcttggctgc tttcttttca ggaagttgga ctttggccag gtttggcttt
     6661 cccagagccg ttcctttctc tgtcctttcc ttgggtcctc atggtgtgcc cattggatcc
     6721 ttggccttgt gatcctctgg gaactggggg ccagttccac ttttgcagcc ttctgtgctg
     6781 gaagagaagc ccagcgccct ggaaggagcc tctttaagtc ccccatgtcg ctttctctct
     6841 ctgctctttt agtgtctgag attgcctttc tttgaatttc ccagtgtttc ttttccttgt
     6901 cccttccctc accaacctgg agttattttg gttgactatg tcctggcttt ggcttctcct
     6961 ggcaggaagt catcaggcat cctctccagg tgagccgaaa ttccaccctc ccaggttgga
     7021 catcatcttt taaacccaat ggtctactcc cctccttctt tatgaaacag tgatttcccg
     7081 tgagtaactc tggttctgat tttttgtacc ggcgcttaaa ttctttctgt agacgttgga
     7141 aagccacaaa gaacgtgact gcagtgagcc tcccactgga gcagccttaa ccaacacttt
     7201 ggccaaagcc cccccacctc ccctgtgtac tgtgtgtgtg tttggtggat acagtattcc
     7261 ttttcagtgt ccctaaagct gtgatgggga gtccccactt acctagaaag cattaccagt
     7321 cacctactct gcattctcag atgtaaacct tgtgtagtgt tctttttgca atgacctatt
     7381 tatttaacct atttatattt atttaatttt tactctgaaa tgtatccagt tacaattgta
     7441 cttgcttaaa gcacatcaga tttgttttgg acaacaccct tgaccatttt aaaactggaa
     7501 aagtgatact gtatccttcc atgggatgga tgctttacag tagtcttatt attaaagggt
     7561 gattaatttg gtcggggtaa aatgttaatt tttaggtgat ttttaagaat tctgtgccat
     7621 tatgtcttct gtgtggatgg ttaattgttt aattagtacg tgttaattgt gtgatacagt
     7681 cttctttgtg gaacccaaaa tcctcttttt agctttatat tttataaact gccagattgt
     7741 acaactttta tgtgcatttt taaagcttga agacatgagg gtcattatct aagttaaaca
     7801 gcctattttt gtgcctcctg tacagtttta taattctgct gatggcggca tcttatgtcg
     7861 agccaaccac aataaaggta gttttagatt ttgga
//
