LOCUS       NM_000082               9414 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens ERCC excision repair 8, CSA ubiquitin ligase complex
            subunit (ERCC8), transcript variant 1, mRNA.
ACCESSION   NM_000082
VERSION     NM_000082.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Yang,D., Lai,A., Davies,A., Janssen,A.F., Ellis,M.O. and Larrieu,D.
  TITLE     A novel role for CSA in the regulation of nuclear envelope
            integrity: uncovering a non-canonical function
  JOURNAL   Life Sci Alliance 7 (11), e202402745 (2024)
   PUBMED   39209536
  REMARK    GeneRIF: A novel role for CSA in the regulation of nuclear envelope
            integrity: uncovering a non-canonical function.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9414)
  AUTHORS   van Sluis,M., Yu,Q., van der Woude,M., Gonzalo-Hansen,C.,
            Dealy,S.C., Janssens,R.C., Somsen,H.B., Ramadhin,A.R.,
            Dekkers,D.H.W., Wienecke,H.L., Demmers,J.J.P.G., Raams,A.,
            Davo-Martinez,C., Llerena Schiffmacher,D.A., van Toorn,M.,
            Hackes,D., Thijssen,K.L., Zhou,D., Lammers,J.G., Pines,A.,
            Vermeulen,W., Pothof,J., Demmers,J.A.A., van den Berg,D.L.C.,
            Lans,H. and Marteijn,J.A.
  TITLE     Transcription-coupled DNA-protein crosslink repair by CSB and
            CRL4CSA-mediated degradation
  JOURNAL   Nat Cell Biol 26 (5), 770-783 (2024)
   PUBMED   38600236
  REMARK    GeneRIF: Transcription-coupled DNA-protein crosslink repair by CSB
            and CRL4[CSA]-mediated degradation.
REFERENCE   3  (bases 1 to 9414)
  AUTHORS   Sabharwal,A., Gupta,V., Kv,S., Kumar Manokaran,R., Verma,A.,
            Mishra,A., Bhoyar,R.C., Jain,A., Sivadas,A., Rawat,S., Jolly,B.,
            Mohanty,S., Gulati,S., Gupta,N., Kabra,M., Scaria,V. and
            Sivasubbu,S.
  TITLE     Whole genome sequencing followed by functional analysis of genomic
            deletion encompassing ERCC8 and NDUFAF2 genes in a
            non-consanguineous Indian family reveals dysfunctional
            mitochondrial bioenergetics leading to infant mortality
  JOURNAL   Mitochondrion 75, 101844 (2024)
   PUBMED   38237647
  REMARK    GeneRIF: Whole genome sequencing followed by functional analysis of
            genomic deletion encompassing ERCC8 and NDUFAF2 genes in a
            non-consanguineous Indian family reveals dysfunctional
            mitochondrial bioenergetics leading to infant mortality.
REFERENCE   4  (bases 1 to 9414)
  AUTHORS   Liu,M.W., Hu,C.F., Jin,J.Y., Xiang,R., Fan,L.L., Li,Y.L. and Zhu,L.
  TITLE     A compound heterozygous mutation of ERCC8 is responsible for a
            family with Cockayne syndrome
  JOURNAL   Mol Biol Rep 51 (1), 371 (2024)
   PUBMED   38411728
  REMARK    GeneRIF: A compound heterozygous mutation of ERCC8 is responsible
            for a family with Cockayne syndrome.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9414)
  AUTHORS   Hao,X.D., Yao,Y.Z., Xu,K.G., Dong,B., Xu,W.H. and Zhang,J.J.
  TITLE     Insufficient Dose of ERCC8 Protein Caused by a Frameshift Mutation
            Is Associated With Keratoconus With Congenital Cataracts
  JOURNAL   Invest Ophthalmol Vis Sci 63 (13), 1 (2022)
   PUBMED   36454558
  REMARK    GeneRIF: Insufficient Dose of ERCC8 Protein Caused by a Frameshift
            Mutation Is Associated With Keratoconus With Congenital Cataracts.
REFERENCE   6  (bases 1 to 9414)
  AUTHORS   Selby,C.P. and Sancar,A.
  TITLE     Human transcription-repair coupling factor CSB/ERCC6 is a
            DNA-stimulated ATPase but is not a helicase and does not disrupt
            the ternary transcription complex of stalled RNA polymerase II
  JOURNAL   J Biol Chem 272 (3), 1885-1890 (1997)
   PUBMED   8999876
REFERENCE   7  (bases 1 to 9414)
  AUTHORS   Bregman,D.B., Halaban,R., van Gool,A.J., Henning,K.A.,
            Friedberg,E.C. and Warren,S.L.
  TITLE     UV-induced ubiquitination of RNA polymerase II: a novel
            modification deficient in Cockayne syndrome cells
  JOURNAL   Proc Natl Acad Sci U S A 93 (21), 11586-11590 (1996)
   PUBMED   8876179
REFERENCE   8  (bases 1 to 9414)
  AUTHORS   Itoh,T., Shiomi,T., Shiomi,N., Harada,Y., Wakasugi,M.,
            Matsunaga,T., Nikaido,O., Friedberg,E.C. and Yamaizumi,M.
  TITLE     Rodent complementation group 8 (ERCC8) corresponds to Cockayne
            syndrome complementation group A
  JOURNAL   Mutat Res 362 (2), 167-174 (1996)
   PUBMED   8596535
REFERENCE   9  (bases 1 to 9414)
  AUTHORS   Henning,K.A., Li,L., Iyer,N., McDaniel,L.D., Reagan,M.S.,
            Legerski,R., Schultz,R.A., Stefanini,M., Lehmann,A.R., Mayne,L.V.
            and Friedberg,E.C.
  TITLE     The Cockayne syndrome group A gene encodes a WD repeat protein that
            interacts with CSB protein and a subunit of RNA polymerase II TFIIH
  JOURNAL   Cell 82 (4), 555-564 (1995)
   PUBMED   7664335
REFERENCE   10 (bases 1 to 9414)
  AUTHORS   Laugel,V.
  TITLE     Cockayne Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301516
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA169067.1, U28413.1 and
            AC104113.3.
            
            On Jan 22, 2020 this sequence version replaced NM_000082.3.
            
            Summary: This gene encodes a WD repeat protein, which interacts
            with Cockayne syndrome type B (CSB) protein and with p44 protein, a
            subunit of the RNA polymerase II transcription factor IIH.
            Mutations in this gene have been identified in patients with
            hereditary disease Cockayne syndrome (CS). CS cells are abnormally
            sensitive to ultraviolet radiation and are defective in the repair
            of transcriptionally active genes. Several transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Mar 2014].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.1127875.1,
                                           SRR1660805.216558.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000676185.1/ ENSP00000501614.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                DA169067.1         9-34
            27-1352             U28413.1           1-1326
            1353-1840           AC104113.3         25702-26189
            1841-2036           U28413.1           1815-2010
            2037-9414           AC104113.3         26386-33763
FEATURES             Location/Qualifiers
     source          1..9414
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q12.1"
     gene            1..9414
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /note="ERCC excision repair 8, CSA ubiquitin ligase
                     complex subunit"
                     /db_xref="GeneID:1161"
                     /db_xref="HGNC:HGNC:3439"
                     /db_xref="MIM:609412"
     exon            1..139
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..1253
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Cockayne syndrome WD-repeat protein CSA; DNA excision
                     repair protein ERCC-8; excision repair cross-complementing
                     rodent repair deficiency, complementation group 8;
                     cockayne syndrome WD repeat protein CSA; excision repair
                     cross-complementation group 8"
                     /codon_start=1
                     /product="DNA excision repair protein ERCC-8 isoform 1"
                     /protein_id="NP_000073.1"
                     /db_xref="CCDS:CCDS3978.1"
                     /db_xref="GeneID:1161"
                     /db_xref="HGNC:HGNC:3439"
                     /db_xref="MIM:609412"
                     /translation="MLGFLSARQTGLEDPLRLRRAESTRRVLGLELNKDRDVERIHGG
                     GINTLDIEPVEGRYMLSGGSDGVIVLYDLENSSRQSYYTCKAVCSIGRDHPDVHRYSV
                     ETVQWYPHDTGMFTSSSFDKTLKVWDTNTLQTADVFNFEETVYSHHMSPVSTKHCLVA
                     VGTRGPKVQLCDLKSGSCSHILQGHRQEILAVSWSPRYDYILATASADSRVKLWDVRR
                     ASGCLITLDQHNGKKSQAVESANTAHNGKVNGLCFTSDGLHLLTVGTDNRMRLWNSSN
                     GENTLVNYGKVCNNSKKGLKFTVSCGCSSEFVFVPYGSTIAVYTVYSGEQITMLKGHY
                     KTVDCCVFQSNFQELYSGSRDCNILAWVPSLYEPVPDDDETTTKSQLNPAFEDAWSSS
                     DEEG"
     exon            140..235
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            236..337
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            338..461
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            462..543
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            544..612
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            613..679
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            680..780
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            781..905
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            906..1103
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1184
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1185..9414
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2012..2017
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /note="hexamer: AATAAA"
     polyA_site      2034
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /note="major polyA site"
     regulatory      4958..4963
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /note="hexamer: AGTAAA"
     polyA_site      4993
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
     regulatory      8226..8231
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /note="hexamer: AATACA"
     polyA_site      8244
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
     regulatory      9389..9394
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
                     /note="hexamer: AATAAA"
     polyA_site      9414
                     /gene="ERCC8"
                     /gene_synonym="CKN1; CSA; UVSS2"
ORIGIN      
        1 agtcccgccc ctgagctctg tcatggcgac gtccagtgct ccagccggtg tgaggacacg
       61 atatgctggg gtttttgtcc gcacgccaaa cgggtttgga ggaccctctt cgccttcgga
      121 gagcagagtc aacacggaga gttttgggac tggaattaaa taaagacaga gatgttgaaa
      181 gaatccacgg cggtggaatt aacacccttg acattgaacc tgttgaaggg agatacatgt
      241 tatcaggtgg ttcagatggt gtgattgtac tttatgacct tgagaactcc agcagacaat
      301 cttattacac atgtaaagca gtgtgttcca ttggcagaga tcatcctgat gttcacagat
      361 acagtgtgga gactgtacag tggtatcctc atgacactgg catgttcaca tcaagctcat
      421 ttgataaaac tctgaaagta tgggatacaa atacattaca aactgcagat gtatttaatt
      481 ttgaggaaac agtttatagt catcatatgt ctccagtctc caccaagcac tgtttggtag
      541 cagttggtac tagaggaccc aaagtacaac tttgtgactt gaagtctgga tcctgttctc
      601 acattctaca gggtcacaga caagaaatat tagcagtttc ctggtctcca cgttatgact
      661 atatcttggc aacagcaagt gctgacagta gagtaaaatt atgggatgtg agaagagcat
      721 caggatgttt gattactctt gatcaacata atgggaaaaa gtcacaagct gttgaatcag
      781 caaacactgc tcataatggg aaagttaatg gcttatgttt tacaagtgat ggacttcacc
      841 tcctcactgt tggtacagat aatcgaatga ggctctggaa tagttccaat ggagaaaaca
      901 cacttgtgaa ctatggaaaa gtttgtaata acagtaaaaa aggattgaaa ttcactgtct
      961 cctgtggctg cagttcagaa tttgtttttg taccatatgg tagcaccatt gctgtttata
     1021 cagtttactc aggagaacag ataactatgc ttaagggaca ttataaaact gttgactgct
     1081 gtgtatttca gtcaaatttc caggaacttt atagtggtag cagagactgc aacattctgg
     1141 cttgggttcc atccttatat gaaccagttc ctgatgatga tgagactaca acaaaatcac
     1201 aattaaatcc ggcctttgaa gatgcctgga gcagcagtga tgaagaagga tgaatatcat
     1261 ctttagtacc tttttgtctc tgctgaaact ttttaaatga gactgtgttt ttttcaactg
     1321 tatggtctat tcctgacagc taaattagcc ctaaatgtgg gtaatatttt tcctcatgtt
     1381 ttaaaatgag gttaatattt gcataaaatc ctaaaacaga cttctgtata gtttatttag
     1441 tcaaaatgtg ttccttgatc ccagatgttg tggcctggga aagccctcat tgctacagta
     1501 caagtaacac aagtcgttgt acctcagttg tgaccttcag cagattttat gaactataag
     1561 atgcagtctc agaggatcag caagtggagg ccatcagtat tgactttctc ttacttgctg
     1621 tactatcagc ctgctcgttt ccacctttaa gaatgatttt gccaagaatg attatatcaa
     1681 aaatagtagt tgaaatggta acatcaaaat tattttattc tttcttcttc atgtattcac
     1741 atttttcagt ggtttcattt aattaaccat gctttatgtt aaacattttg gggctcaatg
     1801 tctcctacta tccaaaatgt gcatcacagg aggcttttaa ctttgtgaaa atcccatgtt
     1861 tgctttattt tattttaatg tcagaaggca gtttgcgcta atgcttgaac tctttttctg
     1921 tgaaactcat taaggtatga ccaaatcctg cctcattaat tcaagcagaa aatatcctgg
     1981 cagggaatct ggcttaaaca tgaaatgctg taataaaatt tctatgttat tgtctccgtc
     2041 tgtgtcaacc cacacgtgat tatcatgaag gtaatcttaa ttttcccttc ctgatcctta
     2101 ccagcagtag actagtaaat gaggctttta tgagcttgaa taccaaaagt aaacagttgt
     2161 tgagggtttt ttgttttttt gtttttttcc tgagatggag tttcactctg tcgctcaggc
     2221 cggagtgcag tgttttgatc tcagctcact gcaaccttca cctcccaggt tcaagcaatt
     2281 ctcctgccta agcctcctga gtagctggga ttacaggctc ccgccactgc gcccggttaa
     2341 tttttttgta ttttcagtag agatggggtt tcaccatgtt ggccaggctg gttgcgaact
     2401 gctgacctca aatgatccac ccgcctcggc ctctcaaagt gctggaatta taagcttgag
     2461 ccactgcacc cagccagttg ttgagttttt aagtgtataa gggttagacc tcctatcaga
     2521 cctttccagc taaatgtttt ccaaacactt gcttttacaa ttgatttttt tttccaattg
     2581 gcagataaaa ttgtatgtat ttaccatgta caacatactg ttttgaagca tatatacatt
     2641 atagaatgac taaatctagc taactgacat atgcctcaca tggttaccat tttgtggtaa
     2701 gaacacttta catccattct cttatttttc aaaattaata gtaatcttgt aattgtaact
     2761 atagttacta tgttgtgcaa taggtctctt gcactaattc ccccttttta actgaaattt
     2821 tgtatccttt gaccaatgtt tctccaacaa ccctagctcc tggtaaccac cattctactc
     2881 tctacttcta tgagatcagc tgtttgtgta tatgtactac attttcttta tccattcaac
     2941 cattgacaga tgcttaggtt gataccatat cctggctatt gtgaataatg ctgcagtgaa
     3001 cctggtagtg cagatatctc tttgacatac tgatttcata tcctttggat ataagcccag
     3061 tattaggatt gctggatcat atgtagttct atttttaact ttttgcagaa actccatact
     3121 gtcttccata atggctgtac taatttacat tcctaccaag aatatgcaag aattcctttt
     3181 tcttcacaat aatgcaagaa atcctttttc ttcacatcct tgccaacact tgttatcttt
     3241 tgtgttattg atagtagcca ttctaacagg tgttaggtgg tatctcgttg ttttaatttg
     3301 catttccctg gtgactgctg atgttgataa ttttttcata tatctgctgg ccatttgcat
     3361 gtcttctttt gagaactgtc ttttcaggtt ctttgcccat ttttaaatgg gttgttttcc
     3421 tgctattgag ttccttatgt attttggata ttaaccccct atcagatcta tagttaatat
     3481 tttttctcca ttctgtaggt tgtctcttca ccctattaat tgtttccttt gctgtgcaga
     3541 agctttttag tttcatgtaa tttcatttct gtacttgtgc ttttgttact gtgcttttga
     3601 gatcgtatcc aaaaaaatca ttgcccagac caatgtcatg gagcattttc tccatgtttt
     3661 cttctgatag tttcaaatca ttgatttatt aagcccaggc cattctactt tcaatcttgt
     3721 ccccttggtg aggatgcatt tgacctttga aaaagctagt ttgaaaaagc tattggccgg
     3781 gcacggtggt tgtaatccta acactttgtg agctgagaca ggcagatcac ttgagctcag
     3841 gagttcaaga ccagcctggg caacatggca aaacctcgtc tctaaataaa aaatacaaaa
     3901 attaaccaag tgtgttgtgc acacctgtag tcccagctac ttgggagact caggctgggg
     3961 aatcacttga acctgtaaag tcacggctgc attgagccaa gatcgtgtca ctacactcca
     4021 gcctgggtga catggcgaga ccttgtgtct cccaaaagaa aaagaaaaga aaagctatta
     4081 aaattggaaa tttattttta agaaaaagga agtctgcttc caaactgcaa gtaactgttt
     4141 tacttacttc tagttatata gatatatagg ctcattataa ataattcaaa caacataaaa
     4201 atctaaaaag gaaggtagtg gtcacctgga attataatac cataaggtaa ccaccactaa
     4261 cattttcatg gtcattcttc atgaatctct tatgatacat ataattttac ataatgggat
     4321 gttcaacaag ctgttgttat aaacacccct ctctttccac attagtacca cattccccag
     4381 tccttacccc tgctgccaca aaccaagtta ttaacctaga ctgtgttctt tcatattgca
     4441 atcctatata tatctatgca gacatatagg ggatgtattt ttattttgca gaactgggat
     4501 atacaaagtt tttaaattat attttgcagt tacagcttat ataaatccct ctctgtcatt
     4561 tgttataaca aagttattct ttttgttaac tgcatcatac tgcattttgt gaatgtcccc
     4621 cagttgtttt cagctggtcc cctgttcaag gctacatgtt tattagtgaa gacttggaac
     4681 tgtactatat ttttcttggt atattttggt gtacaatttg tacaatttag ttgttgaata
     4741 tcatcttttt tttcccctct gaaatgatag tttagctggg tatacgattt ttgggttgta
     4801 gtccttttgt tttgttggtc tatagatatt gtgcccactt ttttctagct tctggtattg
     4861 cagttgagaa ttccaaagcc actttggttt tttttccctt tgtagggaat tcattcgtta
     4921 cagctggaag cttgtaaact gaggtttaaa agaaaaaagt aaaaacttac agtttcttaa
     4981 tctcgtcaag aaatctgatg tcttttttta ttctcttccg tcacttctta gacttcttgg
     5041 gagctctttc aatgaaggtc caaacctttt ctcagctcag gtatattttc ttctaatatt
     5101 tgtctaattg ttgcttctcc tttttccttc ttcttttttg gattatctaa aaattgcatg
     5161 taactgacct ctctatgttt cttcctttct tccctttttt ttttttgaga caaaatctca
     5221 ttctgtcacc cagactagag ggcagtggcg ccatctcagc tcactgcaac ctccgcttcc
     5281 caggttcaag caattttcct gcctcagcct cccaagcagc tgggattaca ggtgcccacc
     5341 accacgcctg gctaattttt tttttttttt tttttttttt tttttagcag aggtggggtt
     5401 tcaccatgtt ggccaggctg gtcttgaact cctgggctct agtgatccac cagcctcggc
     5461 ctcccaaagt gctgggattt acaggcgtga gccaccgtgc cgggcctgac ttcgacttct
     5521 ctttcattgt cttatctttt cgtttctcct tgcccttttt gttttgtttt gtttcaattc
     5581 ttagaactgt agttggcatg tagtaggtgc ttaataatta cttgttttgt tgtttctgtc
     5641 ctatactttg agagtttgtg tttgatattc aaggttacta attcaggcct gactgtgata
     5701 attaggggag acttttgatt attgttttat tcccctcccc accctaaacc cttgttttgt
     5761 tttgttttgt tttgttaagc acttgcaaaa atatagtgga gaaatatggg aggcttccac
     5821 ttgaaacttt ttaataattg tttattcaac tggtatttgt caacccttat tcacattgta
     5881 tatcaatgaa tctattatga aggcagcgat catgtataac ttctctccag tgccttgtac
     5941 attttatatg ttttcctttc acgctgtctt ggctgtcctt tcgtcaccta aggtacttat
     6001 attctgccat atttcaaaga atacttaggc catttgttca cttcactttg attccatttt
     6061 taactagtca ttatggtaca cctatgaaat gcctagtttt aaaaaaatat gtagaaataa
     6121 taaatgcaca ttttattaac atagccataa atctattttt aaccagaagc ctaaaaaatc
     6181 acatcgtaga tcagcttgac ctctctttta aacttgctac catgccttta ctttagaatt
     6241 aagggaacac cttaatagag aaaaaaagtt atcccatgaa ctgaagtgtt ccttgtgtcc
     6301 agtttatact aaaaagcccc atttaaaata gattttaaaa cctttaaaaa attctctaaa
     6361 gacaaaacct taaactgtac caattataat aatagataaa gtaatttcaa aacgaagctc
     6421 ttcagtaaga gacacatagg tttgtagagc tcagtattgg tgagagacac accattagct
     6481 ttattattta aaatcgaaat gagacagtgg tagaaaaata ttgcatcagc aaactaatct
     6541 caattatttt tcaaagcaaa atcactattt cacaatggaa aggaaaacct caaattaggt
     6601 acttaaataa gggctacaca gttttcattc agaaatattt cattactgtt gagtaatata
     6661 ctaatacttt gttatttcta taaaaatgat ataactactg aagactagag aagctaaaga
     6721 cgtttctgtg aattctataa tttttataaa caaatacttt ggtaaatttc cattttttta
     6781 atttaatgtt ttccatgttc aggtgctaat tattgagccc gaactaaaaa acttatcact
     6841 aaagcagtaa acttaaaatc actctaacta tgccagaaat ggaatggcaa gcctagcaca
     6901 attagaatat tgcctgtcga aaaatatagc agggaaagct aattacagaa aggaaaacaa
     6961 tcactttgct ttctattgta atgaatttct ggacatcgga aggttaaatc tagggctcag
     7021 gatagtgcta gttcttctgt tttaattctg gaaaagtatt aaattcaggg aagaaaacta
     7081 acgaagttta atcataaaca ttttttcttc tttgcaaaga atatgtgtgt agttcttaat
     7141 gtgctaccta gaatcactgt tgtaaacctc ctgggtgctt agaattatta caaatcattt
     7201 attataatac aaatttttct atcttgctag ctctatcagc aacatcctca gatacagaag
     7261 gcatacttct tttgctgcca tgtggcttct tgttttttgt ttttattaaa agtaatcaca
     7321 aaaactgcaa ttacttttgc accaacctaa taattagaca attaaaattt caaaggaagg
     7381 gggtaaaatt aaagaactat gttgcgacct ctcttagttc tctgtgtgta ggacttttca
     7441 ggtgcccaat gtcctcttga ctgaccactc ctcatgaaga tactggtaaa ctttgtgcta
     7501 gtggattgat aaccgctctc cgagttcatg ctcttctgag gtctagaaag aaatgggtgt
     7561 ctctgtccca acctggttag ttattctgtc tctaaaaaca caattaagtc aatgttaaga
     7621 ctaagttcat taagctgctc gtgtttttat gccttgacag tttgttaatg gtggttgcat
     7681 aagttttaca gaactagttt cgtcttgttg cccaggctgg agggcaatgg tgtgacctca
     7741 gctcaccgca acctccgcct cctgggttca agcgattctc ctgcctcagc ctcccaagta
     7801 gctgggatta caggcatgcg ccaccatgcc tggcttattt tgtattttta gtagagacag
     7861 ggtttctcca tgttggtctg gctggtctcg aactcctgac ctcaggtgat ctgcccgcct
     7921 tgacctccca aagtgcaggg attacaggca tgagccaccg cggccagcct gtagtcagct
     7981 ctttaaggca acaatttatt ggctcaggtg actggcacaa gccagttttc ttttgttcat
     8041 ttcatccatt gtggcaagga ttagcaacag tactaaatga gagcttgtca acaccttctg
     8101 taaaaacacc accaggagca ggagagctgc ttctcccatg tggctcaaac acttaattcc
     8161 agcttctcta caccctgcat gatggtatca tccactattg tgtaaatctg tatattttct
     8221 aaataaatac actgttcaaa cttatgctaa aacaaagaac attcctaata agtctttgag
     8281 aactgagtta ttaactttta ttgtaatatt ctaatggttt cttacatata tatgcaaaat
     8341 tcagagtatt atggaaaaag ttattagaat aactcagtga tgatcactat ggacattaaa
     8401 gaatggtaag attatttttt tcattgtaat ggctttataa agaaataagg ccaggcgcag
     8461 tggctcacac ctgtaaaccc agcactttgg gcatcagagg caggagaatg gcctgaaccc
     8521 tgaaggcgga ggttgcagtg agccgagatc gccccactgc actccaccct gagtgacaga
     8581 gcaagactct gtctcaaaaa aatgaaaaga aaaagaaata acagtggact ggaaatcaaa
     8641 agtcctgagt ctgtcttagg tcactaccaa ggaaacatat acatttggat attttttttc
     8701 cttctttttt cattggttaa aaatgaagga agggctgggc atggtggctc acgcctataa
     8761 tcccagcact ttgggaggct gaggtggatg gatcacgagg tcaggagatc gagaccatcc
     8821 tggctaacat ggtgaaaccc cgtctttact aaaaatacaa aaaaaaaatt aggcgggcac
     8881 ttgtagtccc agctatttgg gaggctgagg caggagaatg gcgtgaaccc cggaggcagc
     8941 gcttgcagta agccgagatt gcgccactgc actccggcct ggacaagaga gcgagactcc
     9001 atctcaaaaa aaaaaggaag gggacagaaa actggaatag aaaattatta aggttcattc
     9061 ctgctctaaa atcacaaatc atcatatttt ctaaagcatt taaaaatggt atgcataaat
     9121 aattagaggc ttaggttcta aactaattat aattaaagtt cattgaaggc agaggtagag
     9181 tgtcattata aaagttttat gaattgtctt aatactgagt gaacactcaa attctaatgg
     9241 aatgaatttt gcctgaatta cacttttaaa gcaaagtttc catgtgaatt attatggagc
     9301 catcattgat tacctatttg attttgcaag tttattcatt tgtagaacgg gaataaaata
     9361 caccttacaa agttgtaaga ataaatgaaa taaagttctg aaatataaga atca
//
