LOCUS       NM_020207              10245 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens ERCC excision repair 6 like 2 (ERCC6L2), transcript
            variant 1, mRNA.
ACCESSION   NM_020207 XM_351841
VERSION     NM_020207.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10245)
  AUTHORS   Carnie,C.J., Armstrong,L., Sebesta,M., Ariza,A., Wang,X.,
            Graham,E., Zhu,K. and Ahel,D.
  TITLE     ERCC6L2 mitigates replication stress and promotes centromere
            stability
  JOURNAL   Cell Rep 42 (4), 112329 (2023)
   PUBMED   37014751
  REMARK    GeneRIF: ERCC6L2 mitigates replication stress and promotes
            centromere stability.
REFERENCE   2  (bases 1 to 10245)
  AUTHORS   Baccelli,F., Leardini,D., Cerasi,S., Messelodi,D., Bertuccio,S.N.
            and Masetti,R.
  TITLE     ERCC6L2-related disease: a novel entity of bone marrow failure
            disorder with high risk of clonal evolution
  JOURNAL   Ann Hematol 102 (4), 699-705 (2023)
   PUBMED   36790458
  REMARK    GeneRIF: ERCC6L2-related disease: a novel entity of bone marrow
            failure disorder with high risk of clonal evolution.
            Review article
REFERENCE   3  (bases 1 to 10245)
  AUTHORS   Armes,H., Bewicke-Copley,F., Rio-Machin,A., Di Bella,D.,
            Philippe,C., Wozniak,A., Tummala,H., Wang,J., Ezponda,T.,
            Prosper,F., Dokal,I., Vulliamy,T., Kilpivaara,O.,
            Wartiovaara-Kautto,U., Fitzgibbon,J. and Rouault-Pierre,K.
  TITLE     Germline ERCC excision repair 6 like 2 (ERCC6L2) mutations lead to
            impaired erythropoiesis and reshaping of the bone marrow
            microenvironment
  JOURNAL   Br J Haematol 199 (5), 754-764 (2022)
   PUBMED   36156210
  REMARK    GeneRIF: Germline ERCC excision repair 6 like 2 (ERCC6L2) mutations
            lead to impaired erythropoiesis and reshaping of the bone marrow
            microenvironment.
REFERENCE   4  (bases 1 to 10245)
  AUTHORS   Ewing,A.D., Cheetham,S.W., McGill,J.J., Sharkey,M., Walker,R.,
            West,J.A., West,M.J. and Summers,K.M.
  TITLE     Microdeletion of 9q22.3: A patient with minimal deletion size
            associated with a severe phenotype
  JOURNAL   Am J Med Genet A 185 (7), 2070-2083 (2021)
   PUBMED   33960642
  REMARK    GeneRIF: Microdeletion of 9q22.3: A patient with minimal deletion
            size associated with a severe phenotype.
REFERENCE   5  (bases 1 to 10245)
  AUTHORS   Francica,P., Mutlu,M., Blomen,V.A., Oliveira,C., Nowicka,Z.,
            Trenner,A., Gerhards,N.M., Bouwman,P., Stickel,E., Hekkelman,M.L.,
            Lingg,L., Klebic,I., van de Ven,M., de Korte-Grimmerink,R.,
            Howald,D., Jonkers,J., Sartori,A.A., Fendler,W., Chapman,J.R.,
            Brummelkamp,T. and Rottenberg,S.
  TITLE     Functional Radiogenetic Profiling Implicates ERCC6L2 in
            Non-homologous End Joining
  JOURNAL   Cell Rep 32 (8), 108068 (2020)
   PUBMED   32846126
  REMARK    GeneRIF: Functional Radiogenetic Profiling Implicates ERCC6L2 in
            Non-homologous End Joining.
REFERENCE   6  (bases 1 to 10245)
  AUTHORS   Shabanova,I., Cohen,E., Cada,M., Vincent,A., Cohn,R.D. and Dror,Y.
  TITLE     ERCC6L2-associated inherited bone marrow failure syndrome
  JOURNAL   Mol Genet Genomic Med 6 (3), 463-468 (2018)
   PUBMED   29633571
  REMARK    GeneRIF: ERCC6L2-associated disorder has recently been described.
            We identified an additional case through whole-exome sequencing. At
            the age of 9 years, the patient underwent whole exome sequencing
            and was discovered to have a homozygous stop mutation in ERCC6L2
            (NCBI RefSeq NG_034107.1), c.1687C>T (p.Arg563*).
            Review article
REFERENCE   7  (bases 1 to 10245)
  AUTHORS   Jarviaho,T., Halt,K., Hirvikoski,P., Moilanen,J., Mottonen,M. and
            Niinimaki,R.
  TITLE     Bone marrow failure syndrome caused by homozygous frameshift
            mutation in the ERCC6L2 gene
  JOURNAL   Clin Genet 93 (2), 392-395 (2018)
   PUBMED   28815563
  REMARK    GeneRIF: We report 2 cases of bone marrow failure with no
            extra-hematopoietic manifestations in patients from unrelated
            families with a homozygous truncating mutation in ERCC6L2. Bone
            marrow failure without developmental delay or microcephaly with
            ERCC6L2 mutation has not been previously described.
REFERENCE   8  (bases 1 to 10245)
  AUTHORS   Zhang,S., Pondarre,C., Pennarun,G., Labussiere-Wallet,H., Vera,G.,
            France,B., Chansel,M., Rouvet,I., Revy,P., Lopez,B., Soulier,J.,
            Bertrand,P., Callebaut,I. and de Villartay,J.P.
  TITLE     A nonsense mutation in the DNA repair factor Hebo causes mild bone
            marrow failure and microcephaly
  JOURNAL   J Exp Med 213 (6), 1011-1028 (2016)
   PUBMED   27185855
  REMARK    GeneRIF: Hebo is ubiquitously expressed, localized in the nucleus,
            and rapidly recruited to DNAdsb's in an NBS1-dependent manner.
REFERENCE   9  (bases 1 to 10245)
  AUTHORS   Tummala,H., Kirwan,M., Walne,A.J., Hossain,U., Jackson,N.,
            Pondarre,C., Plagnol,V., Vulliamy,T. and Dokal,I.
  TITLE     ERCC6L2 mutations link a distinct bone-marrow-failure syndrome to
            DNA repair and mitochondrial function
  JOURNAL   Am J Hum Genet 94 (2), 246-256 (2014)
   PUBMED   24507776
  REMARK    GeneRIF: These observations identify a distinct bone-marrow-failure
            syndrome due to mutations in ERCC6L2, a gene implicated in DNA
            repair and mitochondrial function.
REFERENCE   10 (bases 1 to 10245)
  AUTHORS   Vaz Meirelles,G., Ferreira Lanza,D.C., da Silva,J.C., Santana
            Bernachi,J., Paes Leme,A.F. and Kobarg,J.
  TITLE     Characterization of hNek6 interactome reveals an important role for
            its short N-terminal domain and colocalization with proteins at the
            centrosome
  JOURNAL   J Proteome Res 9 (12), 6298-6316 (2010)
   PUBMED   20873783
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL161454.10 and AL159167.23.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Oct 22, 2019 this sequence version replaced NM_020207.6.
            
            Summary: This gene encodes a member of the Snf2 family of
            helicase-like proteins. The encoded protein may play a role in DNA
            repair and mitochondrial function. Mutations in this gene have been
            associated with bone marrow failure syndrome 2. Alternatively
            spliced transcript variants that encode different protein isoforms
            have been described. [provided by RefSeq, Apr 2014].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.1586965.1,
                                           SRR14038192.172501.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000653738.2/
                                                ENSP00000499221.2
            RefSeq Select criteria           :: based on manual assertion,
                                                conservation, expression,
                                                longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-394               AL161454.10        95420-95813         c
            395-819             AL161454.10        90211-90635         c
            820-942             AL161454.10        73533-73655         c
            943-1136            AL161454.10        64233-64426         c
            1137-1298           AL161454.10        55675-55836         c
            1299-1506           AL161454.10        55070-55277         c
            1507-1647           AL161454.10        50189-50329         c
            1648-1761           AL161454.10        49086-49199         c
            1762-1881           AL161454.10        48125-48244         c
            1882-1953           AL161454.10        43354-43425         c
            1954-2099           AL161454.10        42640-42785         c
            2100-2195           AL161454.10        29955-30050         c
            2196-2295           AL161454.10        15491-15590         c
            2296-2448           AL161454.10        4790-4942           c
            2449-2529           AL161454.10        848-928             c
            2530-3685           AL159167.23        2430-3585
            3686-3840           AL159167.23        8558-8712
            3841-4022           AL159167.23        35017-35198
            4023-10245          AL159167.23        42722-48944
FEATURES             Location/Qualifiers
     source          1..10245
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q22.32"
     gene            1..10245
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /note="ERCC excision repair 6 like 2"
                     /db_xref="GeneID:375748"
                     /db_xref="HGNC:HGNC:26922"
                     /db_xref="MIM:615667"
     exon            1..394
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     CDS             349..5001
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /note="isoform a is encoded by transcript variant 1;
                     stretch responsive protein 278; putative repair and
                     recombination helicase RAD26L; excision repair
                     cross-complementing rodent repair deficiency,
                     complementation group 6-like 2; DNA repair and
                     recombination protein RAD26-like; excision repair
                     cross-complementation group 6 like 2; DNA excision repair
                     protein ERCC-6-like 2"
                     /codon_start=1
                     /product="DNA excision repair protein ERCC-6-like 2
                     isoform a"
                     /protein_id="NP_064592.3"
                     /db_xref="CCDS:CCDS94443.1"
                     /db_xref="GeneID:375748"
                     /db_xref="HGNC:HGNC:26922"
                     /db_xref="MIM:615667"
                     /translation="MDPSAPQPRAETSGKDIWHPGERCLAPSPDNGKLCEASIKSITV
                     DENGKSFAVVLYADFQERKIPLKQLQEVKFVKDCPRNLIFDDEDLEKPYFPNRKFPSS
                     SVAFKLSDNGDSIPYTINRYLRDYQREGTRFLYGHYIHGGGCILGDDMGLGKTVQVIS
                     FLAAVLHKKGTREDIENNMPEFLLRSMKKEPLSSTAKKMFLIVAPLSVLYNWKDELDT
                     WGYFRVTVLHGNRKDNELIRVKQRKCEIALTTYETLRLCLDELNSLEWSAVIVDEAHR
                     IKNPKARVTEVMKALKCNVRIGLTGTILQNNMKELWCVMDWAVPGLLGSGTYFKKQFS
                     DPVEHGQRHTATKRELATGRKAMQRLAKKMSGWFLRRTKTLIKDQLPKKEDRMVYCSL
                     TDFQKAVYQTVLETEDVTLILQSSEPCTCRSGQKRRNCCYKTNSHGETVKTLYLSYLT
                     VLQKVANHVALLQAASTSKQQETLIKRICDQVFSRFPDFVQKSKDAAFETLSDPKYSG
                     KMKVLQQLLNHCRKNRDKVLLFSFSTKLLDVLQQYCMASGLDYRRLDGSTKSEERLKI
                     VKEFNSTQDVNICLVSTMAGGLGLNFVGANVVVLFDPTWNPANDLQAIDRAYRIGQCR
                     DVKVLRLISLGTVEEIMYLRQIYKQQLHCVVVGSENAKRYFEAVQGSKEHQGELFGIH
                     NLFKFRSQGSCLTKDILEREGQVEAGIMTATTWLKEGPPAHKLEMPRQPDCQECRGTE
                     QAAEPLAKEACDLCSDFSDEEPVGATGIKTAKNKAPDSSKASSSPGQLTLLQCGFSKL
                     LETKCKAVEDSDGNTASDDESSDEQPTCLSTEAKDAGCEKNQDSLGTSKHQKLDNILN
                     PKEKHIFYKSEKILEQNISSKSDEKKIKNTDKHCILQNVTESEDSDVICPTQYTTERF
                     PDNSIRFKPPLEGSEDSETEHTVKTRNNDNSRNTDDKRNGIISKKLSPENTTLKSILK
                     RKGTSDISDESDDIEISSKSRVRKRASSLRFKRIKETKKELHNSPKTMNKTNQVYAAN
                     EDHNSQFIDDYSSSDESLSVSHFSFSKQSHRPRTIRDRTSFSSKLPSHNKKNSTFIPR
                     KPMKCSNEKVVNQEQSYESMDKFLDGVQEVAYIHSNQNVIGSSKAENHMSRWAAHDVF
                     ELKQFSQLPANIAVCSSKTYKEKVDADTLPHTKKGQQPSEGSISLPLYISNPVNQKKK
                     KVYHTNQTTFIIGETPKGIRRKQFEEMASYFNSSSVNEFAKHITNATSEERQKMLRDF
                     YASQYPEVKEFFVDSVSQFNNSSFEKGEQRTRKKSDKRESLIKPRLSDSETLSFKDST
                     NKISQVCSLKTYKRKSVKFQNHISYREEVFFNDAETKKSPVSSTQEIDSGKNSQASED
                     TVTSRSLNSESETRERRLENTMKDQQDLTRTGISRKEPLLKLENKKIENPVLENTSVI
                     SLLGDTSILDDLFKSHGNSPTQLPKKVLSGPMEKAKQRPKDFWDILNEQNDESLSKLT
                     DLAVIETLCEKAPLAAPFKRREEPATSLWKSNEKFLWKKFSPSDTDENATNTQSTT"
     exon            395..819
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            820..942
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1136
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            1137..1298
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1506
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            1507..1647
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            1648..1761
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..1881
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            1882..1953
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            1954..2099
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            2100..2195
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            2196..2295
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            2296..2448
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            2449..2529
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            2530..3685
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            3686..3840
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            3841..4022
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     exon            4023..10245
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5712..5717
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /note="hexamer: AATAAA"
     polyA_site      5730
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /note="major polyA site"
     regulatory      6339..6344
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /note="hexamer: AATAAA"
     polyA_site      6369
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
     regulatory      10226..10231
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /note="hexamer: ATTAAA"
     polyA_site      10245
                     /gene="ERCC6L2"
                     /gene_synonym="BMFS2; C9orf102; HEBO; RAD26L; SR278"
                     /note="major polyA site"
ORIGIN      
        1 agagcctagg aagatttggg ggtcgccttg ccggcctcct gtcctcctcc ggcggcggcg
       61 gagcccgaga gaactaggtg aacaccgctt tgccagcctc acacagcgtc ccctggctct
      121 gccgccgctc cggacgtcgc cctcccgttc tgcttgggtc cccttagtcg ctacctttgc
      181 tgggatcccc ctcctccatc ctgtggcttc gggttgccga agagcgatgc tcggagggcg
      241 gccggaagtg gcgttggccg ccattggcct gccggccagc caccttgctg tcctccgccg
      301 ccttccgggt gttacatgca gccgggctcg gcccctcccc ctggccggat ggatccgtcg
      361 gcgccacagc cccgcgcgga aacctcaggc aaagacatat ggcatccagg agaaagatgt
      421 cttgcccctt ctccagataa tggaaaactt tgtgaagcaa gcataaaatc tatcacagtg
      481 gatgaaaatg gcaagtcatt tgcagtcgtc ttatatgcag attttcaaga aaggaaaata
      541 cctcttaaac agcttcaaga agtgaaattt gttaaagatt gccctaggaa tcttatattt
      601 gatgatgaag atttagaaaa accttatttc ccaaaccgaa aatttccatc atcttctgtt
      661 gcttttaaat tatctgacaa tggagactct attccttata ccatcaatag gtatttgaga
      721 gactaccaaa gagaaggaac ccggtttctt tatggacact acatccatgg aggagggtgc
      781 attctgggtg atgacatggg acttggaaaa acagtacagg ttatttcatt tctggctgca
      841 gttttgcata aaaagggaac tcgtgaggat attgaaaata acatgccaga gtttttacta
      901 agaagtatga aaaaggaacc cctttcttct acagcaaaaa agatgttctt aatagttgct
      961 cctctttctg tcctctacaa ctggaaggat gaattggaca cctggggata tttcagagtc
     1021 actgttttac atggaaacag aaaagataat gaattaattc gtgtaaagca gaggaaatgt
     1081 gaaattgctc taacaactta tgaaacacta cgcttatgcc tggatgaact taacagtttg
     1141 gaatggtcag ctgtcattgt ggatgaagct catagaatca agaatccaaa agctagagta
     1201 acagaagtta tgaaagcttt gaaatgtaat gtccgcattg gcctcactgg aaccatcctt
     1261 cagaacaaca tgaaggaact gtggtgtgtt atggactggg ctgtgccagg ccttttaggg
     1321 agtgggacct acttcaagaa gcagttttct gacccagtag aacatggtca gagacacacg
     1381 gcaacaaaga gagaactagc cactggccga aaggccatgc aaagacttgc caaaaagatg
     1441 tctggctggt ttctcaggcg caccaagact cttatcaagg atcagttgcc taagaaggaa
     1501 gaccggatgg tgtattgttc tttgacagat ttccagaaag ctgtctatca aacagtgtta
     1561 gaaacagagg acgtgacttt gatacttcaa tcttctgagc cttgtacctg taggagtggc
     1621 caaaaaagga gaaattgttg ttataagacc aattctcatg gtgaaacagt gaaaaccttg
     1681 tatctcagtt accttacagt ccttcagaag gtagctaacc atgtcgcgct actgcaagct
     1741 gctagtactt ccaaacaaca ggaaacactt atcaaaagga tatgtgatca ggtattttcc
     1801 agattcccag attttgtgca gaaaagcaaa gatgcagcct ttgaaacact ttctgaccct
     1861 aaatacagtg gaaaaatgaa ggtccttcag cagcttttaa atcattgcag gaaaaacaga
     1921 gataaagttc ttctcttttc tttttccacc aagttgcttg acgtgctaca gcagtactgt
     1981 atggcgtctg ggcttgatta ccgacgactt gatggaagta caaaatcaga ggaaagactc
     2041 aagattgtaa aagagttcaa cagtacacaa gatgttaaca tttgccttgt ctctacaatg
     2101 gctggtggac taggcctcaa ttttgtcggt gccaatgttg ttgtattatt tgatcctact
     2161 tggaatccag ccaatgatct tcaagccatt gacagagcat ataggattgg acaatgtaga
     2221 gatgtcaaag tgcttaggct gatatccttg ggaactgtgg aggaaatcat gtatttacga
     2281 cagatataca agcagcaact tcactgtgtg gtggttggaa gtgaaaatgc caaacgatat
     2341 tttgaagcag ttcaaggatc taaagagcat caaggagagc tttttgggat ccataacctc
     2401 ttcaaattta ggtcccaagg gtcttgtctt acgaaggaca tcctggagag agaaggccaa
     2461 gtagaagcag ggatcatgac agccacaaca tggttgaaag agggacctcc agcacacaaa
     2521 ctggaaatgc ctagacagcc tgactgtcag gaatgcagag gtacagaaca agctgcagag
     2581 ccactggcaa aggaagcatg tgatctctgc agtgacttca gtgatgaaga gccagtggga
     2641 gccacaggaa taaagactgc caaaaacaaa gcacccgatt caagtaaagc ttccagctct
     2701 ccaggacagc ttaccttact ccagtgtggt ttctcgaaat tgcttgaaac aaaatgtaaa
     2761 gcagttgagg atagtgatgg aaatactgcc tctgatgatg aaagttctga tgagcagccc
     2821 acatgccttt caacagaagc caaagatgct ggttgtgaga aaaatcagga ctctcttggt
     2881 acttcaaaac atcagaaatt agataacatc ctaaatccaa aagaaaagca tattttttat
     2941 aaaagtgaga agattttaga acagaatatt tcttccaagt ctgacgagaa aaaaattaaa
     3001 aatacagata aacattgcat tttacagaat gtcacagaat cagaagatag tgatgtcatc
     3061 tgtcctacac aatacacaac tgagagattc cccgacaata gtataaggtt taagccaccc
     3121 ttggaaggat ctgaggattc tgaaacagaa cacactgtaa aaacaagaaa taatgataat
     3181 agtcgaaaca ctgatgacaa aagaaatgga ataatttcaa aaaagttaag tcctgagaac
     3241 acaaccctga aatctatttt gaaaagaaaa ggcaccagtg atatcagtga tgaatctgat
     3301 gacattgaaa tttcttccaa gtcaagagta agaaagagag ctagttcatt gaggtttaag
     3361 agaataaaag aaaccaaaaa agaacttcac aattctccca aaacaatgaa caaaacaaac
     3421 caagtgtatg cagcaaatga ggatcataac tctcagttta ttgatgatta ttcatcctca
     3481 gatgagagtt tatccgtcag ccacttcagt ttctctaaac agagccacag accaagaact
     3541 ataagagaca gaactagttt ttcttcaaaa ttgcctagcc ataataagaa aaatagcact
     3601 tttattccaa gaaaaccaat gaaatgttca aatgagaaag ttgttaatca agagcagtcg
     3661 tatgaatcaa tggataaatt tttagatggc gttcaggaag tggcttatat tcactcaaac
     3721 cagaatgtaa ttggatcgag caaagctgaa aatcacatga gccgatgggc agcacatgac
     3781 gtatttgagt tgaagcagtt ttctcagctg cctgctaaca tagctgtttg cagttctaag
     3841 acatataaag aaaaagtgga tgcagataca ttgccacaca caaagaaagg ccagcaaccg
     3901 agtgaaggca gcatttcact tcctctttac atttcaaatc ctgtaaacca gaagaagaaa
     3961 aaagtctacc atacaaacca gaccaccttc ataattggag aaacaccaaa aggaatccgc
     4021 agaaaacaat ttgaagaaat ggcctcttat tttaactcgt cttctgtaaa cgaatttgct
     4081 aaacatataa ccaatgccac atcagaagaa cgacagaaaa tgctaagaga cttttatgct
     4141 tctcaatatc cagaggtaaa agaatttttt gtggattctg tgtcacaatt caacaattct
     4201 tcctttgaga aaggagagca gcgcacccgg aagaaatctg ataaaagaga atctcttata
     4261 aaaccaaggc tgtcagattc tgaaaccttg tcatttaaag attctaccaa caaaatttct
     4321 caagtttgca gcctaaaaac atataaaaga aaatcagtta agtttcagaa tcatatttcc
     4381 tatagagaag aggtgttttt taatgatgca gaaactaaga aatcacctgt tagttctact
     4441 caagagattg acagtgggaa aaacagccag gcatccgaag atactgtgac atcccgttct
     4501 ctgaacagtg agtctgaaac acgtgagaga aggttagaaa ataccatgaa agaccaacag
     4561 gacctcacaa gaacgggcat ttcaagaaaa gaaccccttc tcaaattgga aaacaaaaag
     4621 atagaaaatc cagtgctgga aaatacttct gtgataagct tacttggtga tacctctatt
     4681 cttgatgacc tttttaaaag tcatgggaac agtcccacac aactgccaaa gaaagttctt
     4741 tcagggccca tggaaaaagc aaaacagaga ccaaaagatt tctgggacat cttgaatgag
     4801 cagaatgatg agagtcttag taaactcaca gacttggcag taatagagac tctgtgtgaa
     4861 aaagcacctc tagcagcacc ctttaaaagg agagaagagc cagcaacttc tctttggaaa
     4921 tcaaatgaga aatttttatg gaagaaattt agcccaagtg atacagatga aaacgcaacc
     4981 aatacacaga gtaccacata agcatataaa tgaattactg caccagtaaa ctgctgccat
     5041 cactgtttac ggcactggat tccacactga ttctattatc ttgaacacag ttgttgacat
     5101 atatttttat taaattattg ctttaggatt ttttgaagtc taaagtattg tcatggatct
     5161 gtttttcttg atatttgatt tgatctttca agaatatgat tgtatttata gtataaacct
     5221 ctgttatgaa ttagaaaaga ttctaggttt gttaatagga gacctgggac atctttctta
     5281 ctatattaca taatgatgtg acacttgccc cggtgagcat tgtttcccag tatgaaagat
     5341 gaagagtctg taccgaatca gcatgagtgt ccttccagtt taaaaaagct ttgcttcgct
     5401 ctcctaatgg ctcataggct gaatcatgtc tgcccctcaa atcaggtgta taccaatgtg
     5461 ttttttacta gcacttggga aagttattaa gtattttctt tttccctggg catcatgttc
     5521 tattattatt ttagaaaaaa gtcataattg gtactgaata tatggtatat ataatattaa
     5581 aatggtaatt ttgcaacagc tcaaaattaa aaggttaatg ttatacactt tactatatga
     5641 gctgtgatta ctaccattag ccacagatac cagtgcctca actttttatg tacctattgt
     5701 gatttaatgt aaataaaggt ttgtatagta cttttgtagt tcttaagtat gaagaaatgg
     5761 gtaaactttt tattttgtta gaaactgtta tattttgagt gtaatattta tggtttatag
     5821 caaaatgaat gtgcttattg ttgaatgcat gtatttagaa gcctttactc agcccctgtg
     5881 ttctgtgcta ggagcttgag ctctacaggt aaggcagagc taccggtgaa tgaaaggaaa
     5941 tcatgtcagt gaaaaatcat ggtggaaagc ccctggcatc acatgtgcat gctgtaggca
     6001 ggacctgagc tgcctccgct gcaggttcag atgcaccgct gcagctgtcc ttcagttagt
     6061 tcacagggct gcaagaggag gacacatccc tccagaaaac agcctgagcc gggaactggc
     6121 tgtgctaaag agcactgcta tcaagttgag gagagagggc ttccgtgtac tcaggatgta
     6181 gagtcattgc tcagaagtga acaaaaaatc aaaaacaaaa gtcttctcaa gggactgatc
     6241 ggccaagtat gcttttcttt agagcaatgt tttgccctag agaattgtaa aatttatgtc
     6301 atgactcagt acatatgtgt tcgtacatat atgattggaa taaaatgttt atgaaatatt
     6361 tactcataag ccatgtaaca tactttgaca tttttctctt ctaggattgt gtttggtagg
     6421 gcagtgggtt tgtgtgtgtg ttaatcctct cacaacccac tgaattgaat ttcatggccc
     6481 atggttaaga tccacagtgt gaaaacgctg ttttaaatta tatgagttcg tcatttgttt
     6541 gctctgtgca gctgagttgt gtccagactt accagatggt atgttttgcc attgaggggc
     6601 cttctacaca atgagtgcat gatatggtcc ttgatagact tgacttgtag atgttttcag
     6661 cctacaatgt gatcagctat ctgaggaact ccagtaagta gataccactt catttcagtt
     6721 tatatacaag acaatgtagt tcaaacattt taataccttg taaattatga tattcatata
     6781 aatattagct ctatagtctt catatatgta cagttttttt tttttttttt ttttttttga
     6841 gattgagtct cactctgtca cccaggctgg agtacagtgg catgatcttg gctcactgca
     6901 acctccacct ccctcccaaa caactcttat gcctcagcct cctgagtagc taggattaca
     6961 ggtgtgtacc accacactca gctaaacatt tttttttttt tttgagacgg agtctcactc
     7021 tgtcgccagg ctggagtgca gtggctcgat cttggctcac tgcagcctcc gccccccgag
     7081 tcaagcaatt ctcctgcctc agcctcccga gtagctggga ctacaggtgc gtgccaccac
     7141 gcccagctaa tttttttgtg tttttagtag ggatggggtt tcaccatgtt ggccaggctg
     7201 gtctcaatct cctggctgca agcgatccac ctgccttgtc ctcccaaagt gctgggatta
     7261 caggtgtgag ccaccatgcc cagccaataa atgctattaa agaaactttt aggccgggca
     7321 cagtggctca cgcctgtaat cccagcactt tgggaggccg aggcaggcgg atcacaaggt
     7381 catgagatcg agactatcct ggctaacgcg gtgaaacccc gtctctacta aaaaaaaaaa
     7441 aaatacaaaa attagccagg cgtggtggtg ggcgcctgta gtcccagcta ctcgggaggc
     7501 tgaggcagga gaatggcgtg aacctgggag gcagagcttg cagtgagcca ggattgtgcc
     7561 cctgcacccc agcctgggtg acagagcaag actccatctc aaaaaaggaa acttttatca
     7621 atagtagtca aaatgggagc aaaaatgcag aactggagtt taaaaattag tttccaacag
     7681 ttgtcagtga aatatttatc actattatgt aggaaagtgt gtcaggtgga atgcagagtc
     7741 cagtatgaaa aggagcctgt ttcagaacgg aaactgtcag gtggagtgga ctccctgcaa
     7801 tggagtgatg gaaagatttg gtacaaccat tgatgacctg agaacagaca ctaggaaggt
     7861 ttgacaagat ccttcctacc cccactccac tggtgatgtg tcaccaagtt ctgctaacta
     7921 aatgggtggc ctcaagcaaa tcccttacct ccttttagcc ttagtttccc ctttaaattg
     7981 gtgatggatt agatcagtgg tggtcagcca ttttcttaaa gcaactgaac tgtatcttaa
     8041 tcagaaactt aactagtaaa acaattaaaa tgcaagtctg ttgcttcaag caacaccact
     8101 gtggctccat ggagcactaa tttgaaaacc acagagatga gatcatttct aagaaaacct
     8161 ttcaactttg tttccatgat gctacttgcc acaatctttt ttacttaagc aatgataccg
     8221 tttctggctg aacactcacc acacagctat taatattgga aactgaggca agataccaag
     8281 agatttttgc acttgaattc ctcagggttc tcttacagcc ctgaaaacac ctcttcaagg
     8341 aaataaataa catctgcaaa tggtgcagct gcccctaagt attcaaggca aagaagcctt
     8401 agtttgcaga gatgatgata aacttttcat ccttccacag aaaatgagaa agccacagga
     8461 aatacacggt tattttctaa ttaaagttca atgtgtacgc ttttaaattc tgaagttatc
     8521 cttcttttat gatatcaaaa tagccctctt ttttgaaatg atggtagatc ataccttttg
     8581 gtctttttca ttgtccttac ataaaagttg gtaatcatat gtcagtccca gaagttttat
     8641 ttgacactta ctggtctata aaatccaaag gaatggggat cactgcctgc cttaaaggtt
     8701 tttttgtgtg tttgtttgtt tgtttgtttg catgtgaagg atttttatgt cacaaatcca
     8761 gctaacagtg gatctaggat ctaggcctca ccgaggatgt cactgaacct gtcagcccca
     8821 atgacaggca cacaccttca aagagcaaga gtgcccatca ctttctaggc tccttgcctg
     8881 ctgctcattc catcatcacg gatacagact tgtttctggt ttcatcttcc accagagatt
     8941 gcaaagcatc ctctgcccag tgcttctagg aggccttact gggatggttc ataagccact
     9001 ttcctcttgg tgacagcccc tgtacactgt ggcatgtacg gctggctcca cttggtattt
     9061 gttgcaggag ggaagaacat ctcacttttg cttggctaaa gggccacccc aggagagcta
     9121 ttttgcctca gggtcttgca gggtttctct aggaaaacag tgcatctgat agttactctc
     9181 tgcccctagg aggaaagaca gattcgccct tccttcccaa gtcccaggct gtgtggttcc
     9241 cagccccaag ggtagcaagt cagaacctcc ggagaggctt ttaagcatgc acatgtccag
     9301 ggtcctcatc aagagactga atcagggggt ctagggctca gcacaggcac cctttttgtt
     9361 tttgcagttt cacaggaatt ctgatgtgcc agtgattgtc aggtttgtcc ctgggcagga
     9421 cccacctgcc tgaccttctg tccccctggg acacagtatt tccagagagg tgcttcagtt
     9481 gccccaccac gacttccagc gccccccatc tctgtatgca gctgagctca tgatggagcc
     9541 cactgtgtgc tttttgttgt tgttacattc ctataaaaat gtccacgcat atttgaacat
     9601 ctgtgttcat agtggcatta ttcacaatgg ctacaacacg aagaaagcat tccaagtacc
     9661 tatcgatgaa taagcaaaat attacacaca caggaatatt attcagcctt gaaaggaaat
     9721 tcggacacgt gctacaacat agatgaatct tgaggacggt atgcaaagtg aaataaatca
     9781 gacacagaag gacaaatact gtatgattcc acttatataa agtacctaga ttagtcagat
     9841 tcatagagac aaagtagaat ggtggttgct agacgctgga gggaggggag aaaggggagt
     9901 tattaatggg tgtagagttt ctgttacaca ggatgcaaag tggcctgaag atggatggtc
     9961 gtgatggttg cacagcaatg tgaatttaat gacactgaag tgtacactta aaagtcatta
    10021 aaatgctaaa ttttgtatgt gttttacaat tttaaaaatg gaaaaaaagt aagaccaaaa
    10081 gcaacacctc tccagaatgt gtgttatata ccaaattttg atgtatgtga gattgctgac
    10141 tatattttac ttatcaattt gaaattatat tgtttttatg ttgacactcc atataagcca
    10201 ttattttgaa aaaaacattt tccttattaa aagattggaa aaaaa
//
