LOCUS       NM_000124               9001 bp    mRNA    linear   PRI 07-OCT-2024
DEFINITION  Homo sapiens ERCC excision repair 6, chromatin remodeling factor
            (ERCC6), transcript variant 3, mRNA.
ACCESSION   NM_000124
VERSION     NM_000124.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Batenburg,N.L., Sowa,D.J., Walker,J.R., Andres,S.N. and Zhu,X.D.
  TITLE     CSB and SMARCAL1 compete for RPA32 at stalled forks and
            differentially control the fate of stalled forks in BRCA2-deficient
            cells
  JOURNAL   Nucleic Acids Res 52 (9), 5067-5087 (2024)
   PUBMED   38416570
  REMARK    GeneRIF: CSB and SMARCAL1 compete for RPA32 at stalled forks and
            differentially control the fate of stalled forks in BRCA2-deficient
            cells.
REFERENCE   2  (bases 1 to 9001)
  AUTHORS   van Sluis,M., Yu,Q., van der Woude,M., Gonzalo-Hansen,C.,
            Dealy,S.C., Janssens,R.C., Somsen,H.B., Ramadhin,A.R.,
            Dekkers,D.H.W., Wienecke,H.L., Demmers,J.J.P.G., Raams,A.,
            Davo-Martinez,C., Llerena Schiffmacher,D.A., van Toorn,M.,
            Hackes,D., Thijssen,K.L., Zhou,D., Lammers,J.G., Pines,A.,
            Vermeulen,W., Pothof,J., Demmers,J.A.A., van den Berg,D.L.C.,
            Lans,H. and Marteijn,J.A.
  TITLE     Transcription-coupled DNA-protein crosslink repair by CSB and
            CRL4CSA-mediated degradation
  JOURNAL   Nat Cell Biol 26 (5), 770-783 (2024)
   PUBMED   38600236
  REMARK    GeneRIF: Transcription-coupled DNA-protein crosslink repair by CSB
            and CRL4[CSA]-mediated degradation.
REFERENCE   3  (bases 1 to 9001)
  AUTHORS   Newman,J.C., Bailey,A.D., Fan,H.Y., Pavelitz,T. and Weiner,A.M.
  TITLE     An abundant evolutionarily conserved CSB-PiggyBac fusion protein
            expressed in Cockayne syndrome
  JOURNAL   PLoS Genet 4 (3), e1000031 (2008)
   PUBMED   18369450
  REMARK    GeneRIF: a domesticated PiggyBac-like transposon PGBD3, residing
            within intron 5 of the CSB gene, functions as an alternative 3'
            terminal exon
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9001)
  AUTHORS   Henning,K.A., Li,L., Iyer,N., McDaniel,L.D., Reagan,M.S.,
            Legerski,R., Schultz,R.A., Stefanini,M., Lehmann,A.R., Mayne,L.V.
            and Friedberg,E.C.
  TITLE     The Cockayne syndrome group A gene encodes a WD repeat protein that
            interacts with CSB protein and a subunit of RNA polymerase II TFIIH
  JOURNAL   Cell 82 (4), 555-564 (1995)
   PUBMED   7664335
REFERENCE   5  (bases 1 to 9001)
  AUTHORS   Troelstra,C., Hesen,W., Bootsma,D. and Hoeijmakers,J.H.
  TITLE     Structure and expression of the excision repair gene ERCC6,
            involved in the human disorder Cockayne's syndrome group B
  JOURNAL   Nucleic Acids Res 21 (3), 419-426 (1993)
   PUBMED   8382798
REFERENCE   6  (bases 1 to 9001)
  AUTHORS   Laugel,V.
  TITLE     Cockayne Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301516
REFERENCE   7  (bases 1 to 9001)
  AUTHORS   Troelstra,C., van Gool,A., de Wit,J., Vermeulen,W., Bootsma,D. and
            Hoeijmakers,J.H.
  TITLE     ERCC6, a member of a subfamily of putative helicases, is involved
            in Cockayne's syndrome and preferential repair of active genes
  JOURNAL   Cell 71 (6), 939-953 (1992)
   PUBMED   1339317
REFERENCE   8  (bases 1 to 9001)
  AUTHORS   Troelstra,C., Landsvater,R.M., Wiegant,J., van der Ploeg,M.,
            Viel,G., Buys,C.H. and Hoeijmakers,J.H.
  TITLE     Localization of the nucleotide excision repair gene ERCC6 to human
            chromosome 10q11-q21
  JOURNAL   Genomics 12 (4), 745-749 (1992)
   PUBMED   1349298
REFERENCE   9  (bases 1 to 9001)
  AUTHORS   Fryns,J.P., Bulcke,J., Verdu,P., Carton,H., Kleczkowska,A. and Van
            den Berghe,H.
  TITLE     Apparent late-onset Cockayne syndrome and interstitial deletion of
            the long arm of chromosome 10 (del(10)(q11.23q21.2))
  JOURNAL   Am J Med Genet 40 (3), 343-344 (1991)
   PUBMED   1951442
REFERENCE   10 (bases 1 to 9001)
  AUTHORS   Troelstra,C., Odijk,H., de Wit,J., Westerveld,A., Thompson,L.H.,
            Bootsma,D. and Hoeijmakers,J.H.
  TITLE     Molecular cloning of the human DNA excision repair gene ERCC-6
  JOURNAL   Mol Cell Biol 10 (11), 5806-5813 (1990)
   PUBMED   2172786
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC073366.4, DA457690.1,
            L04791.1, AB209504.1 and AL138760.14.
            
            On Nov 22, 2018 this sequence version replaced NM_000124.3.
            
            Summary: This gene encodes a DNA-binding protein that is important
            in transcription-coupled excision repair. The encoded protein has
            ATP-stimulated ATPase activity, interacts with several
            transcription and excision repair proteins, and may promote complex
            formation at DNA repair sites. Mutations in this gene are
            associated with Cockayne syndrome type B and
            cerebrooculofacioskeletal syndrome 1. Alternative splicing occurs
            between a splice site from exon 5 of this gene to the 3' splice
            site upstream of the open reading frame (ORF) of the adjacent gene,
            piggyback-derived-3 (GeneID:267004), which activates the
            alternative polyadenylation site downstream of the
            piggyback-derived-3 ORF. The resulting transcripts encode a fusion
            protein that shares sequence with the product of each individual
            gene. [provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (3) represents the longest
            transcript and encodes the longer protein (isoform 2).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853566.3374.1, L04791.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000355832.10/ ENSP00000348089.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-81                AC073366.4         164279-164359
            82-309              DA457690.1         1-228
            310-4808            L04791.1           215-4713
            4809-6815           AB209504.1         9021-11027
            6816-9001           AL138760.14        92849-95034         c
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q11.23"
     gene            1..9001
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /note="ERCC excision repair 6, chromatin remodeling
                     factor"
                     /db_xref="GeneID:2074"
                     /db_xref="HGNC:HGNC:3438"
                     /db_xref="MIM:609413"
     exon            1..160
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            161..596
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             175..4656
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /EC_number="3.6.4.-"
                     /note="isoform 2 is encoded by transcript variant 3;
                     Cockayne syndrome group B protein; DNA excision repair
                     protein ERCC-6; excision repair cross-complementation
                     group 6; ATP-dependent helicase ERCC6; cockayne syndrome
                     protein CSB; excision repair cross-complementing rodent
                     repair deficiency, complementation group 6; ERCC6-PGBD3
                     fusion protein; Chimeric CSB-PGBD3 protein; Chimeric
                     ERCC6-PGBD3 protein"
                     /codon_start=1
                     /product="DNA excision repair protein ERCC-6 isoform 2"
                     /protein_id="NP_000115.1"
                     /db_xref="CCDS:CCDS7229.1"
                     /db_xref="GeneID:2074"
                     /db_xref="HGNC:HGNC:3438"
                     /db_xref="MIM:609413"
                     /translation="MPNEGIPHSSQTQEQDCLQSQPVSNNEEMAIKQESGGDGEVEEY
                     LSFRSVGDGLSTSAVGCASAAPRRGPALLHIDRHQIQAVEPSAQALELQGLGVDVYDQ
                     DVLEQGVLQQVDNAIHEASRASQLVDVEKEYRSVLDDLTSCTTSLRQINKIIEQLSPQ
                     AATSRDINRKLDSVKRQKYNKEQQLKKITAKQKHLQAILGGAEVKIELDHASLEEDAE
                     PGPSSLGSMLMPVQETAWEELIRTGQMTPFGTQIPQKQEKKPRKIMLNEASGFEKYLA
                     DQAKLSFERKKQGCNKRAARKAPAPVTPPAPVQNKNKPNKKARVLSKKEERLKKHIKK
                     LQKRALQFQGKVGLPKARRPWESDMRPEAEGDSEGEESEYFPTEEEEEEEDDEVEGAE
                     ADLSGDGTDYELKPLPKGGKRQKKVPVQEIDDDFFPSSGEEAEAASVGEGGGGGRKVG
                     RYRDDGDEDYYKQRLRRWNKLRLQDKEKRLKLEDDSEESDAEFDEGFKVPGFLFKKLF
                     KYQQTGVRWLWELHCQQAGGILGDEMGLGKTIQIIAFLAGLSYSKIRTRGSNYRFEGL
                     GPTVIVCPTTVMHQWVKEFHTWWPPFRVAILHETGSYTHKKEKLIRDVAHCHGILITS
                     YSYIRLMQDDISRYDWHYVILDEGHKIRNPNAAVTLACKQFRTPHRIILSGSPMQNNL
                     RELWSLFDFIFPGKLGTLPVFMEQFSVPITMGGYSNASPVQVKTAYKCACVLRDTINP
                     YLLRRMKSDVKMSLSLPDKNEQVLFCRLTDEQHKVYQNFVDSKEVYRILNGEMQIFSG
                     LIALRKICNHPDLFSGGPKNLKGLPDDELEEDQFGYWKRSGKMIVVESLLKIWHKQGQ
                     RVLLFSQSRQMLDILEVFLRAQKYTYLKMDGTTTIASRQPLITRYNEDTSIFVFLLTT
                     RVGGLGVNLTGANRVVIYDPDWNPSTDTQARERAWRIGQKKQVTVYRLLTAGTIEEKI
                     YHRQIFKQFLTNRVLKDPKQRRFFKSNDLYELFTLTSPDASQSTETSAIFAGTGSDVQ
                     TPKCHLKRRIQPAFGADHDVPKRKKFPASNISVNDATSSEEKSEAKGAEVNAVTSNRS
                     DPLKDDPHMSSNVTSNDRLGEETNAVSGPEELSVISGNGECSNSSGTGKTSMPSGDES
                     IDEKLGLSYKRERPSQAQTEAFWENKQMENNFYKHKSKTKHHSVAEEETLEKHLRPKQ
                     KPKNSKHCRDAKFEGTRIPHLVKKRRYQKQDSENKSEAKEQSNDDYVLEKLFKKSVGV
                     HSVMKHDAIMDGASPDYVLVEAEANRVAQDALKALRLSRQRCLGAVSGVPTWTGHRGI
                     SGAPAGKKSRFGKKRNSNFSVQHPSSTSPTEKCQDGIMKKEGKDNVPEHFSGRAEDAD
                     SSSGPLASSSLLAKMRARNHLILPERLESESGHLQEASALLPTTEHDDLLVEMRNFIA
                     FQAHTDGQASTREILQEFESKLSASQSCVFRELLRNLCTFHRTSGGEGIWKLKPEYC"
     exon            597..717
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            718..826
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            827..1571
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1700
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1859
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1860..1995
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1996..2166
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2343
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2460
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2461..2556
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2557..2772
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2773..2883
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2884..3003
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            3004..3098
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            3099..3244
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            3245..3952
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            3953..4157
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            4158..4236
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            4237..9001
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6792..6797
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /note="hexamer: AATAAA"
     polyA_site      6821
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /note="major polyA site"
     regulatory      7299..7304
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /note="hexamer: AATAAA"
     polyA_site      7320
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
     regulatory      8202..8207
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /note="hexamer: ATTAAA"
     polyA_site      8230
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
     regulatory      8399..8404
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /note="hexamer: AATATA"
     polyA_site      8422
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
     regulatory      8970..8975
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
                     /note="hexamer: AATAAA"
     polyA_site      9001
                     /gene="ERCC6"
                     /gene_synonym="ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3;
                     POF11; RAD26; UVSS1"
ORIGIN      
        1 gccacgggcc tccccgcacc agcagaagtc ggagtcgctg ttgggggcgg tgtctatggt
       61 tgagctgagg gcgcaggcgc cacggcccgt cgagctgggt tccaaggcgg ctggcggcgg
      121 tagcgtctct gtttccttgt gggcgctcgc gcggccctgg gtagtctgta gagaatgcca
      181 aatgagggaa tcccccactc aagtcaaact caggagcaag actgtttaca gagtcaacct
      241 gtcagtaata atgaagaaat ggcaatcaag caagaaagtg gtggtgatgg ggaggtggag
      301 gagtacctct cctttcgttc tgtgggtgac gggctgtcca cctctgctgt ggggtgcgca
      361 tcagcagctc cgaggagagg gccagccctg ctgcacatcg accgacatca gatccaggca
      421 gtagagccta gcgcccaggc ccttgagctg cagggtttgg gtgtggacgt ctatgaccag
      481 gacgtgctgg aacagggagt gcttcagcag gtggacaatg ccatccatga ggccagccgt
      541 gcctcccagc tcgttgacgt ggagaaggag tatcggtcgg tcctggatga cctcacgtca
      601 tgtacgacat ccctaaggca aatcaataaa attattgaac agcttagccc tcaagctgcc
      661 accagcagag acatcaacag gaaactagat tctgtaaaac gacagaagta taataaggaa
      721 caacagctaa aaaagatcac tgcaaaacaa aagcatctcc aggccatcct tggaggagca
      781 gaggtgaaaa ttgaactaga tcacgccagt ctggaggagg atgcagagcc ggggccatcc
      841 agtcttggca gcatgctcat gcctgtccag gagactgcct gggaagagct catccgcact
      901 ggccagatga caccttttgg tacccagatc cctcagaaac aggagaaaaa gcccagaaaa
      961 atcatgctta atgaagcatc aggcttcgaa aagtatttgg cagatcaagc aaaactgtct
     1021 tttgaaagga agaagcaagg ttgtaataaa agagcagcta gaaaagctcc agccccagtc
     1081 acgcctccag ccccagtgca aaataaaaac aaaccaaaca agaaagccag agttctgtcc
     1141 aaaaaagagg agcgtttgaa aaagcacatc aagaaactcc agaagagggc tttgcagttc
     1201 caggggaaag tgggattgcc aaaggcaagg agaccttggg agtcagacat gaggccagag
     1261 gcagagggag actctgaggg tgaagagtct gagtatttcc ccacagagga ggaggaagag
     1321 gaggaagatg acgaggtgga gggggcagag gcggacctgt ctggagatgg tactgactat
     1381 gagctgaagc ctctgcccaa gggcgggaaa cggcagaaga aagtgccagt gcaggagatt
     1441 gatgatgact ttttcccaag ttctggggaa gaagctgaag ctgcttctgt aggagaagga
     1501 ggaggaggag gtcggaaagt gggaagatac cgagatgatg gagatgaaga ttattataag
     1561 cagcggttaa ggagatggaa taaactgaga ctgcaggaca aagagaaacg tctgaagctg
     1621 gaggacgatt ctgaggaaag tgatgctgaa tttgacgaag gttttaaagt gccaggtttt
     1681 ctgttcaaaa agctttttaa gtaccagcag acaggtgtta ggtggctgtg ggaattgcac
     1741 tgccagcagg caggaggaat tctgggagat gaaatgggat tgggcaagac catccagata
     1801 attgccttct tggcaggtct gagctacagc aagatcagga ctcgtggttc aaattacagg
     1861 tttgaggggt tgggtccaac tgtaattgtc tgtccaacaa cagtgatgca tcagtgggtg
     1921 aaggaatttc acacgtggtg gcctccgttc agagtggcaa ttctacatga aaccggttcc
     1981 tatacccaca aaaaggagaa actaattcga gatgttgctc attgtcatgg aattttgatc
     2041 acatcttact cctacattcg attgatgcag gatgacatta gcaggtatga ctggcactat
     2101 gtgatcttgg acgaaggaca caaaattcga aatccaaatg ctgctgtcac ccttgcttgc
     2161 aaacagtttc gcacccctca tcggatcatt ctgtctggct caccgatgca aaataacctc
     2221 cgagagctgt ggtcgctctt tgacttcatc ttcccgggaa agttaggcac gttgcctgtg
     2281 tttatggagc agttctccgt ccccatcacc atggggggat attcaaatgc ttccccagta
     2341 caggtcaaaa ctgcttacaa gtgtgcatgt gtcttacgag ataccataaa tccataccta
     2401 ctgcggagaa tgaagtcaga tgtcaagatg agcctttctt tgccagataa aaatgaacag
     2461 gtcttatttt gccgtcttac agatgagcag cataaagtct accaaaattt cgttgattcc
     2521 aaagaagttt acaggattct caatggagag atgcagattt tctccggact tatagcccta
     2581 agaaaaattt gcaaccaccc tgatctcttt tctggaggtc ccaagaatct caaaggtctt
     2641 cctgatgatg aactagaaga agatcagttt gggtactgga aacgttctgg gaaaatgatt
     2701 gttgttgagt ctttgttgaa aatatggcac aagcagggtc agcgagtatt gctgttttct
     2761 cagtcaaggc agatgctgga catacttgaa gtattcctta gagcccaaaa gtatacctat
     2821 ctcaagatgg atggtaccac tacaatagct tcaagacagc cactgattac gagatacaat
     2881 gaggacacat ccatatttgt gtttcttctg accacgcggg tgggcggctt aggtgtcaac
     2941 ctgacggggg caaacagagt tgtcatctat gacccagact ggaacccaag cacggacacg
     3001 caggcccggg agcgagcatg gagaataggc cagaagaagc aagtgactgt gtacaggctc
     3061 ctgactgcgg gcaccattga agaaaagatc taccaccgac aaatcttcaa gcagtttttg
     3121 acaaatagag tgctaaaaga cccaaaacaa aggcggtttt tcaaatccaa tgatctctat
     3181 gagctattta ctctgactag tcctgatgca tcccagagca ctgaaacaag tgcaattttt
     3241 gcaggaactg gatcagatgt tcagacaccc aaatgccatc taaaaagaag gattcaacca
     3301 gcctttggag cagaccatga tgttccaaaa cgcaagaagt tccctgcttc taacatatct
     3361 gtaaatgatg ccacatcatc tgaagagaaa tctgaggcta aaggagctga agtaaatgca
     3421 gtaacttcta atcgaagtga tcctttgaaa gatgaccctc acatgagtag taatgtaact
     3481 agcaatgata ggcttggaga agagacaaat gcagtatctg gaccagaaga gttgtcagtg
     3541 attagtggaa atggggaatg ttcaaattct tcaggaacag gcaaaacttc tatgccatct
     3601 ggtgatgaaa gcattgatga aaagttaggt ctttcttaca aaagagaaag acccagccag
     3661 gctcaaacag aagctttttg ggagaataaa caaatggaaa ataattttta taagcacaag
     3721 tcaaaaacaa aacatcatag tgtggcagaa gaagagaccc tggagaaaca tctgagacca
     3781 aagcaaaagc ctaagaactc taagcattgc agagacgcca agtttgaagg aactcgaatt
     3841 ccacacctgg tgaagaaaag gcgttaccag aagcaagaca gtgaaaacaa gagtgaggcc
     3901 aaggaacaga gcaatgacga ttatgttttg gaaaagcttt tcaaaaaatc agttggcgtg
     3961 cacagtgtca tgaagcacga tgccatcatg gatggagcca gcccagatta tgtactggtg
     4021 gaggcagaag ccaaccgagt ggcccaggat gccctgaaag cactgaggct ctctcgtcag
     4081 cggtgtctgg gagcagtgtc tggtgttccc acctggactg gccacagggg gatttctggt
     4141 gcaccagcag gaaaaaagag tagatttggt aagaaaagga attctaactt ctctgtgcag
     4201 catccttcat caacatctcc aacagagaag tgccaggatg gcatcatgaa aaaggaggga
     4261 aaagataatg tccctgagca ttttagtgga agagcagaag atgcagactc ttcatccggg
     4321 cccctcgctt cctcctcact cttggctaaa atgagagcta gaaaccacct gattctgcca
     4381 gagcgtttag aaagtgaaag cgggcacctg caggaagctt ctgccctgct gcccaccaca
     4441 gaacacgatg accttctggt ggagatgaga aacttcatcg ctttccaggc ccacactgat
     4501 ggccaggcca gcaccaggga gatactgcag gagtttgaat ccaagttatc tgcatcacag
     4561 tcttgtgtct tccgagaact attgagaaat ctgtgcactt tccatagaac ttctggtggt
     4621 gaaggaattt ggaaactcaa gccagaatac tgctaaacaa cattgcttcc taaactttca
     4681 agtccctttt tctaacgggc atttctgatt attaatttat tattaataat catgtttgtc
     4741 aatggaagtt ggctgcactt gatgtttgtt tgcatgatgt ctacctcaga attaaaactt
     4801 taaggaagaa gaaactcttc tctgaaagtt aaaagtttta ataatgctag ctaaaggaga
     4861 aaatacttgg attgattttt ttttttttgg caatctaatt atattgtaaa tcaggtacct
     4921 aacagttact ccttggagca catttgttcc tttacccaaa agatgctgtc agggagcaca
     4981 gttagaagtt tgcagaacag aaatctcaat attttttttt attggtgcta aaaacaggtc
     5041 ttacattcag tcagacctgt tcaataagtt catcaatatc tgataacagc attattttga
     5101 tgcttaaact ttaaacattt atatttacca tttgccaccc acaaaggtca ggtttgttat
     5161 ttgttgtttg ataattatat taattttctt ggaaagatcc tcttttcaag gtactggtaa
     5221 attggtgagt atttttatta gtaaagcatg aaatagtatg gtaataaatg ataagacatg
     5281 tatttgtgga aagctgtagg gtattcagtt taccctggct ttcctttaag cagagggcat
     5341 ctttttctct cctacagtca caaaatgtgt tatcattaaa aaaaatcaaa ttaaagccaa
     5401 aagtaggtac ataaaaacca cacacatgca tgcacacaaa catcactgca gcccacagca
     5461 gacccagccg ttgttaccat gaagtgacac cactccaggc ctctcttgtc tgcaggctgg
     5521 caggctgtct tctctccagt tgccttcgtc ttgcgcctgc ctttgcattc cttgcgacgg
     5581 gctttcttgt ttctgcggtt tggattccag ccaaggctgt ttgtatctca ctactgttta
     5641 tgtgtttgtg gttctgtgat ggtgttgctt tgatcctcag tttattttct tacccatgtt
     5701 tttcttgttt ccttctcagg atgattttat catctcatct ttgaagtgtt gttttccgaa
     5761 attcatcgta ttcctgaaat ttcttcttag ctgtcttagt gcagtttgtt tcttggattt
     5821 gtattctctg gcatgctctt ttcctctctc tcatttttct gtagtatgcc tgccctccta
     5881 ccctgctatt tctttacatc tctctcatgc ttaacatgga tagctgtgtc cagatcttct
     5941 gtctgctcat ccatgtgact cagagaggag ggttctgggc aggggggcct tgccggactg
     6001 catgagagga catgagtttt gctttctctg ctctaatatt ttgcttaagc caagaatcct
     6061 tttcttagag atgttctata tgattcctgt caggattttc tagttttttt tggattatag
     6121 cttgttcatt tcttttgttt ttagtttggt ttatatataa tgagggaaga agatgattac
     6181 attatttttg tcactttgcc atcattgttt agaagtcata gaaagaattt ttaaataggc
     6241 caataagtct taaacttgag tacttggctt agaagaaagt caaaactcct tcctttttga
     6301 ctaagtggtt tgtttctggg gagctcttaa tttctatttt tataatcatt agcctataag
     6361 gaaattgtgt cttccttgtt ctcagggtga tctgctgacc ttgttcactc atgaagcatt
     6421 tgggtatcat acttatagtg tctgaaacat aaactgtatt gagctagaca aggtatagcc
     6481 tcctcttcaa gtagcaaata ctatcaaaag ctataatgca gtaggagcaa ggtggtcctt
     6541 gttccagttt ttgtctcagt tctgctgctg atgtaccatg atcttgggaa ggtggtgtct
     6601 cagtgtggag atctgacaca ttgttaccgt gcctcctggc tggagggact tggagaacaa
     6661 tgcagttaag tagaatggtt ttaacaatac agagaaattt attcatttag ataaaaatct
     6721 gatttttaga actttaaaag ctttgtacag tgtaaataga tttaatgtat ttaacatgct
     6781 ttatcagcac aaataaagga ttttaaaatt ttgtcaaaaa attaaatgtt aatactatca
     6841 ccattaaaaa tgttcaagca atagtctgcc tccccacccc cacaccatct tgcacctgtt
     6901 ccacagctaa gtacagccct aggtttggtg tgtattctcc atgcatttag agaatcacat
     6961 gacacagact gctgctataa tgtcattttc ccattcttcc tttactaata aaatttttga
     7021 gttttacctg gacgtctggc tgctcagctt aagactactt ctcagcctct cttatagtta
     7081 attgtggcca tatgactaag ttttaaactt cccaattggc cctttaaaga agcagggtat
     7141 gcactctcct tgacattccc ctttcttgct ggctgggagg ccaatgggag cttcagtagt
     7201 gacctggcat ctcaagagat ggaagtgatg tgttgaggaa ggagaagttg gcctgccagt
     7261 cttgaactgc tctcctttga cctgttattt aagagtaaaa taaaatactg acttgcttaa
     7321 gctactgtct tttgaagtct attatagcaa ttagtttgct ttaaatatgt aatttatatt
     7381 aatggcctta taccatcctt attttgcaac taacttttca tttaatatta tatgcaagac
     7441 atttctattc ctgtaagtat agctctgcct gcacagaatt gttggattaa tgatgtagat
     7501 tttaaatgtc agtggatgta gcagaattgc ttgcatcaat tcaccctcca taaaaggacc
     7561 catttctcca aacccttgcc aacagtaagt ggtatcaata tcgataggtt gttttgtttt
     7621 gttttaacca gtctcgtgat tgaaagtacc ttgttttcac ttgaatttcc ctgattacgt
     7681 acaaaatcaa acatttccat gtttattggc catttgtaga tctctaccgt acattgccta
     7741 ttagtgttat gttggcctag ttttcctgtg ggttgtttat cttttaggat tgctccccta
     7801 aacaaaaaca aaaaggcagt tattccaatg atcaacaaat attcttttct ccaccagttt
     7861 aaaatgtcag ctttcaactc atcacatgct aagttgtcta tttctgtcct gtggatccag
     7921 gtgttggttc taccaaccac actcctcacg ctgtcatttg gtgctttgca ttgtgttttg
     7981 ccattcatgg gcaagttcct tttcctttct ccccctcttc agtgtcttat acttgggtgt
     8041 tttctgttcc agctgaatca agaattagtt tattaaattc cagtaaaatt gtggtgttct
     8101 tttgactagg attgcatttg tttacacatt aatttgagaa taatagacct ctttataatt
     8161 aatataatgt ttctgattag tgttatgata ggcctcattt tattaaagtc ttttatattc
     8221 ttggatgaaa ttttaataac tttcttcata aatgtcttgc acatttcata ataggcttat
     8281 tcctaggtgt tttatagatt tttagttttg gttgtgagtc ttttaactta cataaaaata
     8341 tggagaatta gcatattcac atatctattt tgtgatgttt atcatacgga agttttaaaa
     8401 tatagtaaat tcagtctttc catttttttt tgtttattta agaaccattc cctagctagg
     8461 gtcatgaata cattcttatg aattattaat cccatatgga tagctagttt ccctagcacc
     8521 tttaaaaaat agtttattat tttcctcctg atgtataagg ccacctttac caatttacat
     8581 ataatgtacg gaaatgtttc ttggctctgc attctgttaa attggtccat ttatctaact
     8641 ttgcttagta acccactgtt tttatcattg tggtatcata attgattatc acaaatggta
     8701 gaatgattgt cctcaaagtc ttttctggta tatattgata taattgtata gcttttcttg
     8761 gtgtagtaaa gtacacagtt aaattttcta acattgaaat atcttttaga ttttcacttc
     8821 cagaatggca atgtgaaggg ccctgtggac ccactgtcct aggaattagc tataactagt
     8881 gaaaattgta acaaaaaata aacaccttcc tctcatacag agagacacaa acacttaatg
     8941 ccactggaaa ttgtcctaaa agcatgtaga ataaagtaac gcttattcaa gaaaatccaa
     9001 a
//
