LOCUS       NM_000502               2719 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens eosinophil peroxidase (EPX), mRNA.
ACCESSION   NM_000502
VERSION     NM_000502.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Xu,L., Huang,X., Chen,Z., Yang,M. and Deng,J.
  TITLE     Eosinophil peroxidase promotes bronchial epithelial cells to
            secrete asthma-related factors and induces the early stage of
            airway remodeling
  JOURNAL   Clin Immunol 263, 110228 (2024)
   PUBMED   38663494
  REMARK    GeneRIF: Eosinophil peroxidase promotes bronchial epithelial cells
            to secrete asthma-related factors and induces the early stage of
            airway remodeling.
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Andreev,D., Kachler,K., Liu,M., Chen,Z., Krishnacoumar,B.,
            Ringer,M., Frey,S., Kronke,G., Voehringer,D., Schett,G. and
            Bozec,A.
  TITLE     Eosinophils preserve bone homeostasis by inhibiting excessive
            osteoclast formation and activity via eosinophil peroxidase
  JOURNAL   Nat Commun 15 (1), 1067 (2024)
   PUBMED   38316791
  REMARK    GeneRIF: Eosinophils preserve bone homeostasis by inhibiting
            excessive osteoclast formation and activity via eosinophil
            peroxidase.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2719)
  AUTHORS   Idler,B.M., Iijima,K., Ochkur,S.I., Jacobsen,E.A., Rank,M.A.,
            Kita,H. and Lal,D.
  TITLE     Eosinophil Peroxidase: A Biomarker for Eosinophilic Chronic
            Rhinosinusitis Agnostic of Polyp Status
  JOURNAL   Laryngoscope 134 (1), 69-78 (2024)
   PUBMED   37255054
  REMARK    GeneRIF: Eosinophil Peroxidase: A Biomarker for Eosinophilic
            Chronic Rhinosinusitis Agnostic of Polyp Status.
REFERENCE   4  (bases 1 to 2719)
  AUTHORS   Lal,D., Wright,B.L., Shim,K.P., Zarka,M.A., Lee,J.J., Chang,Y.H.,
            Ochkur,S.I., Divekar,R., Doyle,A.D., Jacobsen,E.A., Kita,H. and
            Rank,M.A.
  TITLE     Eosinophil peroxidase, GATA3, and T-bet as tissue biomarkers in
            chronic rhinosinusitis
  JOURNAL   J Allergy Clin Immunol 143 (6), 2284-2287 (2019)
   PUBMED   30738839
  REMARK    GeneRIF: EPX is a useful biomarker for eosinophilic inflammation in
            chronic rhinosinusitis.
REFERENCE   5  (bases 1 to 2719)
  AUTHORS   Sakamaki,K., Kanda,N., Ueda,T., Aikawa,E. and Nagata,S.
  TITLE     The eosinophil peroxidase gene forms a cluster with the genes for
            myeloperoxidase and lactoperoxidase on human chromosome 17
  JOURNAL   Cytogenet Cell Genet 88 (3-4), 246-248 (2000)
   PUBMED   10828600
REFERENCE   6  (bases 1 to 2719)
  AUTHORS   Keyhani,E., Zarei,M.A. and Lashgarblooki-Livani,T.
  TITLE     Kinetics of peroxidases in guinea pig bone marrow under
            immunostimulation
  JOURNAL   FEBS Lett 452 (3), 233-236 (1999)
   PUBMED   10386597
REFERENCE   7  (bases 1 to 2719)
  AUTHORS   Oxvig,C., Thomsen,A.R., Overgaard,M.T., Sorensen,E.S., Hojrup,P.,
            Bjerrum,M.J., Gleich,G.J. and Sottrup-Jensen,L.
  TITLE     Biochemical evidence for heme linkage through esters with Asp-93
            and Glu-241 in human eosinophil peroxidase. The ester with Asp-93
            is only partially formed in vivo
  JOURNAL   J Biol Chem 274 (24), 16953-16958 (1999)
   PUBMED   10358043
REFERENCE   8  (bases 1 to 2719)
  AUTHORS   Romano,M., Patriarca,P., Melo,C., Baralle,F.E. and Dri,P.
  TITLE     Hereditary eosinophil peroxidase deficiency: immunochemical and
            spectroscopic studies and evidence for a compound heterozygosity of
            the defect
  JOURNAL   Proc Natl Acad Sci U S A 91 (26), 12496-12500 (1994)
   PUBMED   7809065
REFERENCE   9  (bases 1 to 2719)
  AUTHORS   Sakamaki,K., Tomonaga,M., Tsukui,K. and Nagata,S.
  TITLE     Molecular cloning and characterization of a chromosomal gene for
            human eosinophil peroxidase
  JOURNAL   J Biol Chem 264 (28), 16828-16836 (1989)
   PUBMED   2550461
REFERENCE   10 (bases 1 to 2719)
  AUTHORS   Ten,R.M., Pease,L.R., McKean,D.J., Bell,M.P. and Gleich,G.J.
  TITLE     Molecular cloning of the human eosinophil peroxidase. Evidence for
            the existence of a peroxidase multigene family
  JOURNAL   J Exp Med 169 (5), 1757-1769 (1989)
   PUBMED   2541222
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM147889.1, AC005962.1,
            X14346.1, AL597635.1 and AJ705406.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_000502.5.
            
            Summary: This gene is a member of the peroxidase gene family and is
            expressed in eosinophils. The encoded preproprotein is
            proteolytically processed into covalently attached heavy and light
            chains to form the mature enzyme, which functions as an oxidant.
            The enzyme is released at sites of parasitic infection or allergen
            stimulation to mediate lysis of protozoa or parasitic worms. The
            gene is found in a gene cluster with other peroxidase genes on
            chromosome 17. Mutations in this gene result in eosinophil
            peroxidase deficiency. [provided by RefSeq, Feb 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA2149004,
                              SAMEA2153307 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000225371.6/ ENSP00000225371.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                BM147889.1         3-36
            35-35               AC005962.1         114382-114382
            36-240              BM147889.1         38-242
            241-458             X14346.1           84-301
            459-459             AL597635.1         196-196
            460-2053            X14346.1           303-1896
            2054-2054           AC005962.1         124927-124927
            2055-2082           X14346.1           1897-1924
            2083-2102           AC005962.1         125859-125878
            2103-2367           X14346.1           1943-2207
            2368-2719           AJ705406.1         133-484
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q22"
     gene            1..2719
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /note="eosinophil peroxidase"
                     /db_xref="GeneID:8288"
                     /db_xref="HGNC:HGNC:3423"
                     /db_xref="MIM:131399"
     exon            1..197
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /inference="alignment:Splign:2.1.0"
     CDS             122..2269
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /EC_number="1.11.1.7"
                     /codon_start=1
                     /product="eosinophil peroxidase preproprotein"
                     /protein_id="NP_000493.1"
                     /db_xref="CCDS:CCDS11602.1"
                     /db_xref="GeneID:8288"
                     /db_xref="HGNC:HGNC:3423"
                     /db_xref="MIM:131399"
                     /translation="MHLLPALAGVLATLVLAQPCEGTDPASPGAVETSVLRDCIAEAK
                     LLVDAAYNWTQKSIKQRLRSGSASPMDLLSYFKQPVAATRTVVRAADYMHVALGLLEE
                     KLQPQRSGPFNVTDVLTEPQLRLLSQASGCALRDQAERCSDKYRTITGRCNNKRRPLL
                     GASNQALARWLPAEYEDGLSLPFGWTPSRRRNGFLLPLVRAVSNQIVRFPNERLTSDR
                     GRALMFMQWGQFIDHDLDFSPESPARVAFTAGVDCERTCAQLPPCFPIKIPPNDPRIK
                     NQRDCIPFFRSAPSCPQNKNRVRNQINALTSFVDASMVYGSEVSLSLRLRNRTNYLGL
                     LAINQRFQDNGRALLPFDNLHDDPCLLTNRSARIPCFLAGDTRSTETPKLAAMHTLFM
                     REHNRLATELRRLNPRWNGDKLYNEARKIMGAMVQIITYRDFLPLVLGKARARRTLGH
                     YRGYCSNVDPRVANVFTLAFRFGHTMLQPFMFRLDSQYRASAPNSHVPLSSAFFASWR
                     IVYEGGIDPILRGLMATPAKLNRQDAMLVDELRDRLFRQVRRIGLDLAALNMQRSRDH
                     GLPGYNAWRRFCGLSQPRNLAQLSRVLKNQDLARKFLNLYGTPDNIDIWIGAIAEPLL
                     PGARVGPLLACLFENQFRRARDGDRFWWQKRGVFTKRQRKALSRISLSRIICDNTGIT
                     TVSRDIFRANIYPRGFVNCSRIPRLNLSAWRGT"
     exon            198..291
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /inference="alignment:Splign:2.1.0"
     exon            292..467
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /inference="alignment:Splign:2.1.0"
     exon            468..585
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /inference="alignment:Splign:2.1.0"
     exon            586..715
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /inference="alignment:Splign:2.1.0"
     exon            716..922
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1241
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1402
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1658
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1829
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /inference="alignment:Splign:2.1.0"
     exon            1830..2067
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /inference="alignment:Splign:2.1.0"
     exon            2068..2280
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /inference="alignment:Splign:2.1.0"
     exon            2281..2719
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2699..2704
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /note="hexamer: AATAAA"
     polyA_site      2719
                     /gene="EPX"
                     /gene_synonym="EPO; EPP; EPX-PEN; EPXD"
                     /note="major polyA site"
ORIGIN      
        1 gaagagctgg aggaagtgag aggtcggctg ggggtcctca aagtgagagg ggagcagagg
       61 atcctcccgt gcaggctgtg gatgtcactc acttcccagc tggtgaagcc tcgctgcaga
      121 gatgcatctg ctcccagccc tggcaggggt cctggccaca ctcgtcctcg cccagccctg
      181 tgagggcact gacccagcct cccctggggc agtggagacc tcggtcctgc gagactgcat
      241 agcagaggcc aagttgctgg tggatgctgc ctacaattgg acccagaaga gcatcaagca
      301 gcggcttcgc agcggttcag ccagccccat ggacctcctg tcctacttca aacaaccggt
      361 agcagccacc aggacagttg ttcgggccgc agattatatg catgtggctt tggggctgct
      421 tgaagagaag ttacaacccc agcggtccgg acccttcaat gtcactgatg tgctaacaga
      481 accacagctg cggctgctgt cccaggccag tggctgtgct ctccgggacc aggccgagcg
      541 ctgcagcgac aagtaccgca ccatcactgg acggtgcaac aacaagagga gacccttgct
      601 aggggcctcc aaccaggctc tggctcgctg gctgcccgcc gagtatgagg atgggctgtc
      661 gctccccttc ggctggaccc ccagcaggag gcgcaatggc ttccttctcc ctcttgtccg
      721 ggctgtctcc aaccagattg tgcgcttccc caatgagaga ctgacctccg accgtggccg
      781 agccctcatg ttcatgcagt ggggccagtt cattgaccat gacctggact tctccccgga
      841 gtccccggcc agagtggcct tcactgcagg cgttgactgt gagaggacct gcgcccagct
      901 gcccccctgc tttcccatca agatcccacc caatgacccc cgcatcaaga accagcgtga
      961 ctgcatccct ttcttccgct cggcaccctc atgcccccaa aacaagaaca gagtccgcaa
     1021 ccagatcaac gcgctcacct cctttgtgga cgccagcatg gtgtatggca gtgaggtctc
     1081 cctctcgctg cggctccgca accggaccaa ctacctgggg ctgctggcca tcaaccagcg
     1141 ctttcaagac aacggccggg ccctgctgcc cttcgacaac ctgcacgatg acccctgtct
     1201 cctcaccaac cgctcggcgc gcatcccctg cttcctggca ggtgacaccc gatcaacgga
     1261 aacccccaaa ctggcagcca tgcacaccct ctttatgcga gagcacaacc ggctggccac
     1321 cgagctgaga cgcctgaatc cccggtggaa tggagacaaa ctgtacaatg aggctcggaa
     1381 gatcatgggg gccatggtcc agatcatcac ctaccgagac tttctgcccc tggttctggg
     1441 caaggcccgg gccaggagaa ccctggggca ctacaggggg tactgctcca atgtggaccc
     1501 acgggtggcc aatgtcttca ccctggcctt ccgctttggc cacacaatgc tccagccctt
     1561 catgttccgc ttggacagtc agtaccgggc ctccgcaccc aactcgcatg tcccacttag
     1621 ctctgccttc tttgccagct ggcggatcgt gtatgaaggg ggcatcgacc ccatcctccg
     1681 gggcctcatg gccacccctg ccaagctgaa ccgtcaggat gccatgttag tggatgagct
     1741 ccgggaccgg ctgtttcggc aagtgaggag gattgggctg gacctggcag ctctcaacat
     1801 gcaacgaagc cgggaccacg gccttccagg gtacaatgct tggaggcgct tctgtgggct
     1861 ctcccagccc cggaatttgg cacagcttag ccgggtgctg aaaaaccagg acttggcaag
     1921 gaagttcctg aatttgtatg gaacacctga caacattgac atctggattg gggccatcgc
     1981 tgagcctctt ttgccggggg ctcgagtggg gcctcttctg gcttgtctgt tcgagaacca
     2041 gttcagaaga gcccgagacg gagacaggtt ctggtggcag aaacgaggtg ttttcaccaa
     2101 aagacagcgc aaggccctga gcagaatttc cttgtctcga attatatgtg acaataccgg
     2161 tatcaccacg gtttcaaggg acatcttcag agccaacatc taccctcggg gctttgtgaa
     2221 ctgcagccgt atccccaggt tgaacctatc agcctggcga gggacatgag gcttctgcag
     2281 gagtctatcc caagtctcca acttttggag acaaggggaa ggggaggacc atgaggctgc
     2341 cttgtctccc tggagcaagt gcaggctgct gacgcttctg ctggctacag ctcagagctg
     2401 ggttccccag ccaggagtga aggctggggg ctcctatcag caatggacct tcccgccttg
     2461 ggagcctctt aggtattagg ctatgaatca gcgccacgtg caaaggcttg ggagccaagc
     2521 catgtggtct tgcaccccag gcaagaaaag tcagctggag ggtttacagc actttctact
     2581 gtttcccagc cctccctccc ctccctcacc atgactaaga gaccactcgg tcctagcctc
     2641 cagacacccc acaatactcc tctgagcctg aggccaggca gcatgctctg cttctaccaa
     2701 taaagcactg ctaagggca
//
