LOCUS       NM_004446               4862 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens glutamyl-prolyl-tRNA synthetase 1 (EPRS1), mRNA.
ACCESSION   NM_004446
VERSION     NM_004446.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Khan,D., Ramachandiran,I., Vasu,K., China,A., Khan,K., Cumbo,F.,
            Halawani,D., Terenzi,F., Zin,I., Long,B., Costain,G., Blaser,S.,
            Carnevale,A., Gogonea,V., Dutta,R., Blankenberg,D., Yoon,G. and
            Fox,P.L.
  TITLE     Homozygous EPRS1 missense variant causing hypomyelinating
            leukodystrophy-15 alters variant-distal mRNA m6A site accessibility
  JOURNAL   Nat Commun 15 (1), 4284 (2024)
   PUBMED   38769304
  REMARK    GeneRIF: Homozygous EPRS1 missense variant causing hypomyelinating
            leukodystrophy-15 alters variant-distal mRNA m[6]A site
            accessibility.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Kang,C., Yun,D., Yoon,H., Hong,M., Hwang,J., Shin,H.M., Park,S.,
            Cheon,S., Han,D., Moon,K.C., Kim,H.Y., Choi,E.Y., Lee,E.Y.,
            Kim,M.H., Jeong,C.W., Kwak,C., Kim,D.K., Oh,K.H., Joo,K.W.,
            Lee,D.S., Kim,Y.S. and Han,S.S.
  TITLE     Glutamyl-prolyl-tRNA synthetase (EPRS1) drives tubulointerstitial
            nephritis-induced fibrosis by enhancing T cell proliferation and
            activity
  JOURNAL   Kidney Int 105 (5), 997-1019 (2024)
   PUBMED   38320721
  REMARK    GeneRIF: Glutamyl-prolyl-tRNA synthetase (EPRS1) drives
            tubulointerstitial nephritis-induced fibrosis by enhancing T cell
            proliferation and activity.
REFERENCE   3  (bases 1 to 4862)
  AUTHORS   Kurata,K., James-Bott,A., Tye,M.A., Yamamoto,L., Samur,M.K.,
            Tai,Y.T., Dunford,J., Johansson,C., Senbabaoglu,F., Philpott,M.,
            Palmer,C., Ramasamy,K., Gooding,S., Smilova,M., Gaeta,G., Guo,M.,
            Christianson,J.C., Payne,N.C., Singh,K., Karagoz,K., Stokes,M.E.,
            Ortiz,M., Hagner,P., Thakurta,A., Cribbs,A., Mazitschek,R.,
            Hideshima,T., Anderson,K.C. and Oppermann,U.
  TITLE     Prolyl-tRNA synthetase as a novel therapeutic target in multiple
            myeloma
  JOURNAL   Blood Cancer J 13 (1), 12 (2023)
   PUBMED   36631435
  REMARK    GeneRIF: Prolyl-tRNA synthetase as a novel therapeutic target in
            multiple myeloma.
            Erratum:[Blood Cancer J. 2023 Feb 7;13(1):24. doi:
            10.1038/s41408-023-00793-y.. PMID: 36746923]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4862)
  AUTHORS   Jin,D., Wek,S.A., Kudlapur,N.T., Cantara,W.A., Bakhtina,M.,
            Wek,R.C. and Musier-Forsyth,K.
  TITLE     Disease-associated mutations in a bifunctional aminoacyl-tRNA
            synthetase gene elicit the integrated stress response
  JOURNAL   J Biol Chem 297 (4), 101203 (2021)
   PUBMED   34537243
  REMARK    GeneRIF: Disease-associated mutations in a bifunctional
            aminoacyl-tRNA synthetase gene elicit the integrated stress
            response.
REFERENCE   5  (bases 1 to 4862)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 4862)
  AUTHORS   Kaiser,E., Eberhard,D. and Knippers,R.
  TITLE     Exons encoding the highly conserved part of human glutaminyl-tRNA
            synthetase
  JOURNAL   J Mol Evol 34 (1), 45-53 (1992)
   PUBMED   1556743
REFERENCE   7  (bases 1 to 4862)
  AUTHORS   Cerini,C., Kerjan,P., Astier,M., Gratecos,D., Mirande,M. and
            Semeriva,M.
  TITLE     A component of the multisynthetase complex is a multifunctional
            aminoacyl-tRNA synthetase
  JOURNAL   EMBO J 10 (13), 4267-4277 (1991)
   PUBMED   1756734
REFERENCE   8  (bases 1 to 4862)
  AUTHORS   Norcum,M.T.
  TITLE     Structural analysis of the high molecular mass aminoacyl-tRNA
            synthetase complex. Effects of neutral salts and detergents
  JOURNAL   J Biol Chem 266 (23), 15398-15405 (1991)
   PUBMED   1651330
REFERENCE   9  (bases 1 to 4862)
  AUTHORS   Fett,R. and Knippers,R.
  TITLE     The primary structure of human glutaminyl-tRNA synthetase. A highly
            conserved core, amino acid repeat regions, and homologies with
            translation elongation factors
  JOURNAL   J Biol Chem 266 (3), 1448-1455 (1991)
   PUBMED   1988429
REFERENCE   10 (bases 1 to 4862)
  AUTHORS   Kunze,N., Bittler,E., Fett,R., Schray,B., Hameister,H.,
            Wiedorn,K.H. and Knippers,R.
  TITLE     The human QARS locus: assignment of the human gene for
            glutaminyl-tRNA synthetase to chromosome 1q32-42
  JOURNAL   Hum Genet 85 (5), 527-530 (1990)
   PUBMED   2227938
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC046156.1, BC034797.1,
            AY493416.1, N85252.1 and AA196283.1.
            
            On Nov 23, 2018 this sequence version replaced NM_004446.2.
            
            Summary: Aminoacyl-tRNA synthetases are a class of enzymes that
            charge tRNAs with their cognate amino acids. The protein encoded by
            this gene is a multifunctional aminoacyl-tRNA synthetase that
            catalyzes the aminoacylation of glutamic acid and proline tRNA
            species. Alternative splicing has been observed for this gene, but
            the full-length nature and biological validity of the variant have
            not been determined. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC136465.1, CR933648.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000366923.8/ ENSP00000355890.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1040              BC046156.1         154-1193
            1041-3012           BC034797.1         990-2961
            3013-3521           AY493416.1         2713-3221
            3522-3539           N85252.1           192-209
            3540-4786           AY493416.1         3240-4486
            4787-4862           AA196283.1         7-82                c
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q41"
     gene            1..4862
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /note="glutamyl-prolyl-tRNA synthetase 1"
                     /db_xref="GeneID:2058"
                     /db_xref="HGNC:HGNC:3418"
                     /db_xref="MIM:138295"
     exon            1..163
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..4656
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /EC_number="6.1.1.15"
                     /EC_number="6.1.1.17"
                     /note="glutamate tRNA ligase; glutaminyl-tRNA synthetase;
                     proline-tRNA ligase; prolyl-tRNA synthetase; bifunctional
                     aminoacyl-tRNA synthetase; proliferation-inducing protein
                     32; proliferation-inducing gene 32 protein; cell
                     proliferation-inducing gene 32 protein;
                     glutamatyl-prolyl-tRNA synthetase"
                     /codon_start=1
                     /product="bifunctional glutamate/proline--tRNA ligase"
                     /protein_id="NP_004437.2"
                     /db_xref="CCDS:CCDS31027.1"
                     /db_xref="GeneID:2058"
                     /db_xref="HGNC:HGNC:3418"
                     /db_xref="MIM:138295"
                     /translation="MATLSLTVNSGDPPLGALLAVEHVKDDVSISVEEGKENILHVSE
                     NVIFTDVNSILRYLARVATTAGLYGSNLMEHTEIDHWLEFSATKLSSCDSFTSTINEL
                     NHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLKQKKAPVHVKRWFGFLEAQQAF
                     QSVGTKWDVSTTKARVAPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAA
                     LLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETI
                     MKYAEKLIQEGKAYVDDTPAEQMKAEREQRIDSKHRKNPIEKNLQMWEEMKKGSQFGQ
                     SCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTH
                     ALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGW
                     DDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYV
                     ALLKKEVIPVNVPEAQEEMKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETFSEGEMV
                     TFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHALPIPVICV
                     TYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQ
                     PYEPVSPYSCKEAPCVLIYIPDGHTKEMPTSGSKEKTKVEATKNETSAPFKERPTPSL
                     NNNCTTSEDSLVLYNRVAVQGDVVRELKAKKAPKEDVDAAVKQLLSLKAEYKEKTGQE
                     YKPGNPPAEIGQNISSNSSASILESKSLYDEVAAQGEVVRKLKAEKSPKAKINEAVEC
                     LLSLKAQYKEKTGKEYIPGQPPLSQSSDSSPTRNSEPAGLETPEAKVLFDKVASQGEV
                     VRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKPVSATGAEDKDKKKKEKENKS
                     EKQNKPQKQNDGQRKDPSKNQGGGLSSSGAGEGQGPKKQTRLGLEAKKEENLADWYSQ
                     VITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSALE
                     KEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQ
                     WCNVVRWEFKHPQPFLRTREFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIP
                     VVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIP
                     GEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDK
                     EALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQ
                     FVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKTHMVVANTMEDFQKI
                     LDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELQPGAK
                     CVCGKNPAKYYTLFGRSY"
     exon            164..248
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            249..348
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            349..505
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            506..645
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            646..740
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            741..867
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            868..1060
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1232
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1466
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1551
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..1611
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1612..1722
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1859
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1860..2067
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2068..2180
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2181..2298
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2299..2658
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2659..2892
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2893..3155
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            3156..3207
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            3208..3417
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            3418..3490
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            3491..3570
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            3571..3672
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            3673..3828
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            3829..4026
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            4027..4200
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            4201..4361
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            4362..4440
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            4441..4505
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            4506..4862
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4844..4849
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /note="hexamer: AATAAA"
     polyA_site      4862
                     /gene="EPRS1"
                     /gene_synonym="EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;
                     QARS; QPRS"
                     /note="major polyA site"
ORIGIN      
        1 gaaggctgcg gcggcgtcgg gtacgcgcac acgttgcatc ttcttccttt cgcggggtcc
       61 tccgtagttc tggcacgagc caggcgtact gacaggtgga ccagcggact ggtggagatg
      121 gcgacgctct ctctgaccgt gaattcagga gaccctccgc taggagcttt gctggcagta
      181 gaacacgtga aagacgatgt cagcatttcc gttgaagaag ggaaagagaa tattcttcat
      241 gtttctgaaa atgtgatatt cacagatgtg aattctatac ttcgctactt ggctagagtt
      301 gcaactacag ctgggttata tggctctaat ctgatggaac atactgagat tgatcactgg
      361 ttggagttca gtgctacaaa attatcttca tgtgattcct ttacttctac aattaatgaa
      421 ctcaatcatt gcctgtctct gagaacatac ttagttggaa actccttgag tttagcagat
      481 ttatgtgttt gggccaccct aaaaggaaat gctgcctggc aagaacagtt gaaacagaag
      541 aaagctccag ttcatgtaaa acgttggttt ggctttcttg aagcccagca ggccttccag
      601 tcagtaggta ccaagtggga tgtttcaaca accaaagctc gagtggcacc tgagaaaaag
      661 caagatgttg ggaaatttgt tgagcttcca ggtgcggaga tgggaaaggt taccgtcaga
      721 tttcctccag aggccagtgg ttacttacac attgggcatg caaaagctgc tcttctgaac
      781 cagcactacc aggttaactt taaagggaaa ctgatcatga gatttgatga cacaaatcct
      841 gaaaaagaaa aggaagattt tgagaaggtt atcttggaag atgttgcaat gttgcatatc
      901 aaaccagatc aatttactta tacttcggat cattttgaaa ctataatgaa gtatgcagag
      961 aagctaattc aagaagggaa ggcttatgtg gatgatactc ctgctgaaca gatgaaagca
     1021 gaacgtgagc agaggataga ctctaaacat agaaaaaacc ctattgagaa gaatctacaa
     1081 atgtgggaag aaatgaaaaa agggagccag tttggtcagt cctgttgttt gcgagcaaaa
     1141 attgacatga gtagtaacaa tggatgcatg agagatccaa ccctttatcg ctgcaaaatt
     1201 caaccacatc caagaactgg aaataaatac aatgtttatc caacatatga ttttgcctgc
     1261 cccatagttg acagcatcga aggtgttaca catgccctga gaacaacaga ataccatgac
     1321 agagatgagc agttttactg gattattgaa gctttaggca taagaaaacc atatatttgg
     1381 gaatatagtc ggctaaatct caacaacaca gtgctatcca aaagaaaact cacatggttt
     1441 gtcaatgaag gactagtaga tggatgggat gacccaagat ttcctacggt tcgtggtgta
     1501 ctgagaagag ggatgacagt tgaaggactg aaacagttta ttgctgctca gggctcctca
     1561 cgttcagtcg tgaacatgga gtgggacaaa atctgggcgt ttaacaaaaa ggttattgac
     1621 ccagtggctc cacgatatgt tgcattactg aagaaagaag tgatcccagt gaatgtacct
     1681 gaagctcagg aggagatgaa agaagtagcc aaacacccaa agaatcctga ggttggcttg
     1741 aagcctgtgt ggtatagtcc caaagttttc attgaaggtg ctgatgcaga gactttttcg
     1801 gagggtgaga tggttacatt tataaattgg ggcaacctca acattacaaa aatacacaaa
     1861 aatgcagatg gaaaaatcat atctcttgat gcaaagttga atttggaaaa caaagactac
     1921 aagaaaacca ctaaggtcac ttggcttgca gagactacac atgctcttcc tattccagta
     1981 atctgtgtca cttatgagca cttgatcaca aagccagtgc taggaaaaga cgaggacttt
     2041 aagcagtatg tcaacaagaa cagtaagcat gaagagctaa tgctagggga tccctgcctt
     2101 aaggatttga aaaaaggaga tattatacaa ctccagagaa gaggattctt catatgtgat
     2161 caaccttatg aacctgttag cccatatagt tgcaaggaag ccccgtgtgt tttgatatac
     2221 attcctgatg ggcacacaaa ggaaatgcca acatcagggt caaaggaaaa gaccaaagta
     2281 gaagccacaa aaaatgagac ctctgctcct tttaaggaaa gaccaacacc ttctctgaat
     2341 aataattgta ctacatctga ggattccttg gtcctttaca atagagtggc tgttcaagga
     2401 gatgtggttc gtgaattaaa agccaagaaa gcaccaaagg aagatgtaga tgcagctgta
     2461 aaacagcttt tgtctttgaa agctgaatat aaggagaaaa ctggccagga atataaacct
     2521 ggaaaccctc ctgctgaaat aggacagaat atttcttcta attcctcagc aagtattctg
     2581 gaaagtaaat ctctgtatga tgaagttgct gcacaagggg aggtggttcg taagctaaaa
     2641 gctgaaaaat cccctaaggc taaaataaat gaagctgtag aatgcttact gtccctgaag
     2701 gctcagtata aagaaaaaac tgggaaggag tacatacctg gtcagccccc attatctcaa
     2761 agttcggatt caagcccaac cagaaattct gaacctgctg gtttagaaac accagaagcg
     2821 aaagtacttt ttgacaaagt agcttctcaa ggggaagtag ttcggaaact taaaactgaa
     2881 aaagccccta aggatcaagt agatatagct gttcaagaac tccttcagct aaaggcacag
     2941 tacaagtctt tgataggagt agagtataag cctgtgtcgg ccactggagc tgaggacaaa
     3001 gataagaaga agaaagaaaa agaaaataaa tctgaaaagc agaataagcc tcagaaacaa
     3061 aatgatggcc aaaggaaaga cccttctaaa aaccaaggag gtgggctctc atcaagtgga
     3121 gcaggagaag ggcaggggcc taagaaacag accaggttgg gtcttgaggc aaaaaaagaa
     3181 gaaaatcttg ctgattggta ttctcaggtc atcacaaagt cagaaatgat tgaataccat
     3241 gacataagtg gctgttatat tcttcgtccc tgggcctatg ccatttggga agccatcaag
     3301 gacttttttg atgctgagat caagaaactt ggtgttgaaa actgctactt ccccatgttt
     3361 gtgtctcaaa gtgcattaga gaaagagaag actcatgttg ctgactttgc cccagaggtt
     3421 gcttgggtta caagatctgg caaaaccgag ctggcagaac caattgccat tcgtcctact
     3481 agtgaaacag taatgtatcc tgcatatgca aaatgggtac agtcacacag agacctgccc
     3541 atcaagctca atcagtggtg caatgtggtg cgttgggaat tcaagcatcc tcagcctttc
     3601 ctacgtactc gtgaatttct ttggcaggaa gggcacagtg cttttgctac catggaagag
     3661 gcagcggaag aggtcttgca gatacttgac ttatatgctc aggtatatga agaactcctg
     3721 gcaattcctg ttgttaaagg aagaaagacg gaaaaggaaa aatttgcagg aggagactat
     3781 acaactacaa tagaagcatt tatatctgct agtggaagag ctatccaggg aggaacatca
     3841 catcatttag ggcagaattt ttccaaaatg tttgaaatcg tttttgaaga tccaaagata
     3901 ccaggagaga agcaatttgc ctatcaaaac tcctggggcc tgacaactcg aactattggt
     3961 gttatgacca tggttcatgg ggacaacatg ggtttagtat taccaccccg tgtagcatgt
     4021 gttcaggtgg tgattattcc ttgtggcatt accaatgcac tttctgaaga agacaaagaa
     4081 gcgctgattg caaaatgcaa tgattatcga aggcgattac tcagtgttaa catccgcgtt
     4141 agagctgatt tacgagataa ttattctcca ggttggaaat tcaatcactg ggagctcaag
     4201 ggagttccca ttagacttga agttgggcca cgtgatatga agagctgtca gtttgtagcc
     4261 gtcagacgag atactggaga aaagctgaca gttgctgaaa atgaggcaga gactaaactt
     4321 caagctattt tggaagacat ccaggtcacc cttttcacaa gggcttctga agaccttaag
     4381 actcatatgg ttgtggctaa tacaatggaa gactttcaga agatactaga ttctggaaag
     4441 attgttcaga ttccattctg tggggaaatt gactgtgagg actggatcaa aaagaccact
     4501 gccagggatc aagatcttga acctggtgct ccatccatgg gagctaaaag cctttgcatc
     4561 cccttcaaac cactctgtga actgcagcct ggagccaaat gtgtctgtgg caagaaccct
     4621 gccaagtact acaccttatt tggtcgcagc tactgaggga tgaacgaaag ccccctcttc
     4681 aactcctctc actttttaaa gcattgatat tagtatcttc tcagatacag accgttttat
     4741 gattttttaa aaagtaaaag ttctaaaatg aagtcacaca ggacaattat tcttatgcct
     4801 aagttaacag tggataaaag acttttctgt aaacaactcc agtaataaat atcatgaact
     4861 aa
//
