LOCUS       NM_001198986            1837 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens EPPIN-WFDC6 readthrough (EPPIN-WFDC6), mRNA.
ACCESSION   NM_001198986
VERSION     NM_001198986.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Prakash,T., Sharma,V.K., Adati,N., Ozawa,R., Kumar,N., Nishida,Y.,
            Fujikake,T., Takeda,T. and Taylor,T.D.
  TITLE     Expression of conjoined genes: another mechanism for gene
            regulation in eukaryotes
  JOURNAL   PLoS One 5 (10), e13284 (2010)
   PUBMED   20967262
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK301937.1, AL031663.2 and AA626362.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001198986.1.
            
            Summary: This locus represents naturally occurring read-through
            transcription between the neighboring SPINLW1 (serine peptidase
            inhibitor-like, with Kunitz and WAP domains 1) and WFDC6 (WAP
            four-disulfide core domain 6) genes. The read-through transcript
            encodes a fusion protein that shares sequence identity with the
            products of each individual gene. [provided by RefSeq, Nov 2010].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189667.202549.1, AK301937.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2148874, SAMEA2151119
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            readthrough transcript :: includes exons from GeneID 57119, 140870
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1131              AK301937.1         1-1131
            1132-1659           AL031663.2         100209-100736       c
            1660-1837           AA626362.1         6-183               c
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.12"
     gene            1..1837
                     /gene="EPPIN-WFDC6"
                     /gene_synonym="SPINLW1-WFDC6"
                     /note="EPPIN-WFDC6 readthrough"
                     /db_xref="GeneID:100526773"
                     /db_xref="HGNC:HGNC:38825"
     exon            1..135
                     /gene="EPPIN-WFDC6"
                     /gene_synonym="SPINLW1-WFDC6"
                     /inference="alignment:Splign:2.1.0"
     CDS             45..584
                     /gene="EPPIN-WFDC6"
                     /gene_synonym="SPINLW1-WFDC6"
                     /note="SPINLW1-WFDC6 read-through transcript;
                     SPINLW1-WFDC6 readthrough"
                     /codon_start=1
                     /product="SPINLW1-WFDC6 fusion protein precursor"
                     /protein_id="NP_001185915.1"
                     /db_xref="GeneID:100526773"
                     /db_xref="HGNC:HGNC:38825"
                     /translation="MGSSGLLSLLVLFVLLANVQGPGLTDWLFPRRCPKIREECEFQE
                     RDVCTKDRQCQDNKKCCVFSCGKKCLDLKQDVCEMPKETGPCLAYFLHWWYDKKDNTC
                     SMFVYGGCQGNNNNFQSKANCLNTCKNKQPCPKIKVECEVEEIDQCTKPRDCPENMKC
                     CPFSRGKKCLDFRKASLST"
     exon            136..267
                     /gene="EPPIN-WFDC6"
                     /gene_synonym="SPINLW1-WFDC6"
                     /inference="alignment:Splign:2.1.0"
     exon            268..435
                     /gene="EPPIN-WFDC6"
                     /gene_synonym="SPINLW1-WFDC6"
                     /inference="alignment:Splign:2.1.0"
     exon            436..566
                     /gene="EPPIN-WFDC6"
                     /gene_synonym="SPINLW1-WFDC6"
                     /inference="alignment:Splign:2.1.0"
     exon            567..1837
                     /gene="EPPIN-WFDC6"
                     /gene_synonym="SPINLW1-WFDC6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1815..1820
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPPIN-WFDC6"
                     /gene_synonym="SPINLW1-WFDC6"
                     /note="hexamer: AATAAA"
     polyA_site      1837
                     /gene="EPPIN-WFDC6"
                     /gene_synonym="SPINLW1-WFDC6"
                     /note="major polyA site"
ORIGIN      
        1 attccaaatt cgggaaccac cagaaaggct ggcctctctt caacatggga tcttctggac
       61 ttttgagcct cctggtgcta ttcgtcctct tagcgaatgt ccagggacct ggtctgactg
      121 attggttatt tcccaggaga tgtcccaaaa tcagagaaga atgtgaattc caagaaaggg
      181 atgtgtgtac aaaggacaga caatgccagg acaacaagaa gtgttgtgtc ttcagctgcg
      241 gaaaaaaatg tttagatctc aaacaagatg tatgcgaaat gccaaaagaa actggcccct
      301 gcctggctta ttttcttcat tggtggtatg acaagaaaga taatacttgc tccatgtttg
      361 tctatggtgg ctgccaggga aacaataaca acttccaatc caaagccaac tgcctgaaca
      421 cctgcaagaa taaacagccg tgtcccaaaa tcaaagtgga atgcgaagtg gaagaaatag
      481 accagtgtac caaacccaga gattgcccag aaaacatgaa gtgttgcccg ttcagccgtg
      541 gaaagaaatg tttagacttc agaaaggcct ctctgtctac ataaatccag gatgccttcc
      601 ctgaccacct tagaccgcct acactctttc ttctctgccc atattattga gctctaaaga
      661 agatcatcaa ggaccattcg ttcattctat tcattcaaca aatattgatt ggtgcccctg
      721 acacccatga tgggttccac ttgctcattg tggccaggaa ggtcagaaaa gtttttctgg
      781 gaaagagatc catgatttaa gatctgaaag atgagtactt agaccaaaaa tgggaagaaa
      841 tagcctttca ggccagataa taggatgtgc aaaggctctg tggcaggagg caggatgttc
      901 aggtctagaa aggctagaaa gcagagagtg agcaagagac aagaaacagg aaactggtag
      961 tgtatattca gggcaacttg aatctgtatt cccaagtggc catcctcaag ttttggggtc
     1021 aaataaatgc cctacttaat gatattttct gaatcttgtt actttaggtt gacatacctt
     1081 tcaagacaat aactaggttt tcagtgcttt gataaaacct caaatgtgtg gccttccagc
     1141 caggcatctg tggtttcata ttgtttaagc atttgttgca tccctatgga ttgcagctct
     1201 ctataggttc gctgttgctg aaccaagaaa taaggcatac acagatttct agagaaattg
     1261 taattctata acagattttc aggagatgag gaaggctggt atctttcaga gtacaaagtg
     1321 attttggaat tgaaagaatt tctttgggtt aaagtttgtc actgacttgt gttcctgaac
     1381 catgaagcat gaatatgtgg gctgagaaat agtttctctt gataaataaa caagtaacaa
     1441 attttaaaaa gagaggaaga tgaagctggg ctagttgggg gaaggagagc tggtaaggga
     1501 tctggtcttg atcctaacag caatggatta acctatgttc aaatccctta ccatctgtgt
     1561 ggcctcaaga aagttattta gcctctctaa accttagttt tctcatctgc ataatgagaa
     1621 caatgagagg acctacttta caagattaaa tgttaataga tgtcaactac taagaacact
     1681 aatcaatgct ataattgttg ttttgttgtg ctgacactaa tcaatgctac aattgttgtt
     1741 ttgtcgtgct tcagacctga ggttctaaag aactctattt gtacctcata gcactatggg
     1801 caaaataatt gctcaataaa tgcttaagat cagggta
//
