LOCUS       NM_020398               1964 bp    mRNA    linear   PRI 13-SEP-2020
DEFINITION  Homo sapiens epididymal peptidase inhibitor (EPPIN), transcript
            variant 1, mRNA.
ACCESSION   NM_020398
VERSION     NM_020398.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Scott A, Glasgow A, Small D, Carlile S, McCrudden M, McLean D,
            Brown R, Doherty D, Lundy FT, Hamid UI, O'Kane CM, McAuley DF,
            Brodlie M, Tunney M, Elborn JS, Irwin CR, Timson DJ, Taggart CC and
            Weldon S.
  TITLE     Characterisation of eppin function: expression and activity in the
            lung
  JOURNAL   Eur. Respir. J. 50 (1), 1601937 (2017)
   PUBMED   28705940
  REMARK    GeneRIF: eppin plays a role outside of the reproductive tract;
            eppin may have a role in the innate immune response in the lung
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Stewart MJ, Stewart P and Rivera-Posada J.
  TITLE     De novo assembly of the transcriptome of Acanthaster planci testes
  JOURNAL   Mol Ecol Resour 15 (4), 953-966 (2015)
   PUBMED   25494938
REFERENCE   3  (bases 1 to 1964)
  AUTHORS   Zhang X, Fang J, Xu B, Zhang S, Su S, Song Z, Deng Y, Wang H, Zhao
            D, Niu X and Wang Z.
  TITLE     Correlation of epididymal protease inhibitor and fibronectin in
            human semen
  JOURNAL   PLoS ONE 8 (12), e82600 (2013)
   PUBMED   24358212
  REMARK    GeneRIF: Eppin and Fibronectin bind to each other in human semen
            and on human ejaculated spermatozoa.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1964)
  AUTHORS   Silva EJ, Hamil KG and O'Rand MG.
  TITLE     Interacting proteins on human spermatozoa: adaptive evolution of
            the binding of semenogelin I to EPPIN
  JOURNAL   PLoS ONE 8 (12), e82014 (2013)
   PUBMED   24312623
  REMARK    GeneRIF: EPPIN and SEMG1 rapidly co-evolved in primates
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1964)
  AUTHORS   O'Rand MG, Widgren EE, Wang Z and Richardson RT.
  TITLE     Eppin: an epididymal protease inhibitor and a target for male
            contraception
  JOURNAL   Soc Reprod Fertil Suppl 63, 445-453 (2007)
   PUBMED   17566290
  REMARK    Review article
REFERENCE   6  (bases 1 to 1964)
  AUTHORS   Wang Z, Widgren EE, Sivashanmugam P, O'Rand MG and Richardson RT.
  TITLE     Association of eppin with semenogelin on human spermatozoa
  JOURNAL   Biol. Reprod. 72 (5), 1064-1070 (2005)
   PUBMED   15590901
  REMARK    GeneRIF: in seminal plasma and on spermatozoa following
            ejaculation, Eppin is bound to semenogelin I
REFERENCE   7  (bases 1 to 1964)
  AUTHORS   Yenugu S, Richardson RT, Sivashanmugam P, Wang Z, O'rand MG, French
            FS and Hall SH.
  TITLE     Antimicrobial activity of human EPPIN, an androgen-regulated,
            sperm-bound protein with a whey acidic protein motif
  JOURNAL   Biol. Reprod. 71 (5), 1484-1490 (2004)
   PUBMED   15229136
  REMARK    GeneRIF: This antimicrobial peptide permeabilized outer and inner
            membranes of E. coli, consistent with its ability to induce
            striking morphological alterations of E. coli membranes. Has role
            in innate immune system of human epididymis.
REFERENCE   8  (bases 1 to 1964)
  AUTHORS   Sivashanmugam P, Hall SH, Hamil KG, French FS, O'Rand MG and
            Richardson RT.
  TITLE     Characterization of mouse Eppin and a gene cluster of similar
            protease inhibitors on mouse chromosome 2
  JOURNAL   Gene 312, 125-134 (2003)
   PUBMED   12909348
REFERENCE   9  (bases 1 to 1964)
  AUTHORS   Clauss A, Lilja H and Lundwall A.
  TITLE     A locus on human chromosome 20 contains several genes expressing
            protease inhibitor domains with homology to whey acidic protein
  JOURNAL   Biochem. J. 368 (Pt 1), 233-242 (2002)
   PUBMED   12206714
REFERENCE   10 (bases 1 to 1964)
  AUTHORS   Richardson RT, Sivashanmugam P, Hall SH, Hamil KG, Moore PA, Ruben
            SM, French FS and O'Rand M.
  TITLE     Cloning and sequencing of human Eppin: a novel family of protease
            inhibitors expressed in the epididymis and testis
  JOURNAL   Gene 270 (1-2), 93-102 (2001)
   PUBMED   11404006
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC395362.1 and AF286370.2.
            
            On Jun 2, 2019 this sequence version replaced NM_020398.3.
            
            Summary: This gene encodes an epididymal protease inhibitor, which
            contains both kunitz-type and WAP-type four-disulfide core (WFDC)
            protease inhibitor consensus sequences. Most WFDC genes are
            localized to chromosome 20q12-q13 in two clusters: centromeric and
            telomeric. This gene is a member of the WFDC gene family and
            belongs to the telomeric cluster. The protein can inhibit human
            sperm motility and exhibits antimicrobial activity against E. coli,
            and polymorphisms in this gene are associated with male
            infertility. Read-through transcription also exists between this
            gene and the downstream WFDC6 (WAP four-disulfide core domain 6)
            gene. Two transcript variants encoding different isoforms have been
            found for this gene. [provided by RefSeq, Nov 2014].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1), which is also known as eppin-1 and eppin-3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GQ891395.1, AF286370.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2148093, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match                 :: ENST00000354280.9/
                                                  ENSP00000361746.4
            Protein has antimicrobial activity :: PMID: 15229136
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                DC395362.1         27-49
            24-1964             AF286370.2         1-1941
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.12"
     gene            1..1964
                     /gene="EPPIN"
                     /gene_synonym="CT71; CT72; dJ461P17.2; SPINLW1; WAP7;
                     WFDC7"
                     /note="epididymal peptidase inhibitor"
                     /db_xref="GeneID:57119"
                     /db_xref="HGNC:HGNC:15932"
                     /db_xref="MIM:609031"
     exon            1..135
                     /gene="EPPIN"
                     /gene_synonym="CT71; CT72; dJ461P17.2; SPINLW1; WAP7;
                     WFDC7"
                     /inference="alignment:Splign:2.1.0"
     CDS             45..446
                     /gene="EPPIN"
                     /gene_synonym="CT71; CT72; dJ461P17.2; SPINLW1; WAP7;
                     WFDC7"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; epididymal protease inhibitor; serine peptidase
                     inhibitor-like, with Kunitz and WAP domains 1 (eppin);
                     cancer/testis antigen 72; protease inhibitor WAP7;
                     cancer/testis antigen 71; WAP four-disulfide core domain
                     protein 7; epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="eppin isoform 1 precursor"
                     /protein_id="NP_065131.1"
                     /db_xref="CCDS:CCDS13359.1"
                     /db_xref="GeneID:57119"
                     /db_xref="HGNC:HGNC:15932"
                     /db_xref="MIM:609031"
                     /translation="MGSSGLLSLLVLFVLLANVQGPGLTDWLFPRRCPKIREECEFQE
                     RDVCTKDRQCQDNKKCCVFSCGKKCLDLKQDVCEMPKETGPCLAYFLHWWYDKKDNTC
                     SMFVYGGCQGNNNNFQSKANCLNTCKNKRFP"
     exon            136..267
                     /gene="EPPIN"
                     /gene_synonym="CT71; CT72; dJ461P17.2; SPINLW1; WAP7;
                     WFDC7"
                     /inference="alignment:Splign:2.1.0"
     exon            268..435
                     /gene="EPPIN"
                     /gene_synonym="CT71; CT72; dJ461P17.2; SPINLW1; WAP7;
                     WFDC7"
                     /inference="alignment:Splign:2.1.0"
     exon            436..1964
                     /gene="EPPIN"
                     /gene_synonym="CT71; CT72; dJ461P17.2; SPINLW1; WAP7;
                     WFDC7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      726..731
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPPIN"
                     /gene_synonym="CT71; CT72; dJ461P17.2; SPINLW1; WAP7;
                     WFDC7"
                     /note="hexamer: AGTAAA"
     polyA_site      751
                     /gene="EPPIN"
                     /gene_synonym="CT71; CT72; dJ461P17.2; SPINLW1; WAP7;
                     WFDC7"
                     /note="major polyA site"
ORIGIN      
        1 attccaaatt cgggaaccac cagaaaggct ggcctctctt caacatggga tcttctggac
       61 ttttgagcct cctggtgcta ttcgtcctct tagcgaatgt ccagggacct ggtctgactg
      121 attggttatt tcccaggaga tgtcccaaaa tcagagaaga atgtgaattc caagaaaggg
      181 atgtgtgtac aaaggacaga caatgccagg acaacaagaa gtgttgtgtc ttcagctgcg
      241 gaaaaaaatg tttagatctc aaacaagatg tatgcgaaat gccaaaagaa actggcccct
      301 gcctggctta ttttcttcat tggtggtatg acaagaaaga taatacttgc tccatgtttg
      361 tctatggtgg ctgccaggga aacaataaca acttccaatc caaagccaac tgcctgaaca
      421 cctgcaagaa taaacgcttt ccctgattgg ataaggatgc actggaagaa ctgccagaat
      481 gtggctcatg ctctgagtac tgttcctgta cctgacggat gctccagact ggcttccagt
      541 ttcactctca gcattccaag atcttagccc ttcccagaac agaacgcttg catctacctc
      601 ctcttcctcc atctttggct cttttgatgc acaatatcca tccgttttga tttcatcttt
      661 atgtcccctt tatctccaac ttctagaact cccagtttat acctgtgtca ctctcaattt
      721 tttccagtaa agtacttgat gtagtcctgt gccttgaggc tgagtgattg acatcttttt
      781 ggcacattac actttgcaga gtaagggatc attgaggctt ctggtgacat cattcaaggg
      841 agtgaagaca atcagaactt tgagagttca gagttccagg aatattgtta ttgttatctt
      901 cctctgtttc tacaaaagat tgtaaaaccc tttttaggca agggcccaag gacagtgcct
      961 tagtttggat cctctagaaa ctaaccctgg gaaaagggca tgggtgcagg tagtttgttt
     1021 gagagatttc ccaggacata caagatgggg agtgagatga gtaattcagg gaagagaagg
     1081 gaagagagaa gagtctatta tgagctggtt actactgtgg ctcaatccta ttggggacct
     1141 tttaaagaat catgtaaaac aggcttacaa attttctttt taaaaatatt ttattttatt
     1201 ttacgttccg ggatacatgt gcaggacctg caggtttgtt acataggtaa gcctgtgcca
     1261 tggtggtttg ctgcacctat caacccatta cctgggtatt aagccccgca tgcattagct
     1321 atttatcctg atgctttccc tccccctgcc ccctcaacag tccacgtgtt ctcattgttc
     1381 atctcccact tgcaagtgag aacatgtggt atttactttt gttcctgcgt tactttgctg
     1441 aggataacgg cttccagctt catccatgtc cctgcaaagg acgtgatctc atttcttttt
     1501 atggctgcat agtattccat ggcatatatg taccacattt tctttatcca gcctatcgtt
     1561 gatggatatt tggtttgatt ccatgttttt gctattgtaa atagtgctgc agtgaacata
     1621 cacatgcatg tatctttata aaagaatgat ttatattcct ttgagtgtat acccagtaat
     1681 gggattgctg ggtcaaatgg tatttctggt tctagatctt tcaggaattg gcacactctc
     1741 ttccacaatg gttgaaccaa tttacattcc caacagtgta aaagcattca tgtttctcca
     1801 cagctttacc agcatctgtt gtttctggac tttttaataa tcaccattct gactggcatg
     1861 agatggtatc tcattgtggt tttgatttgc atttctctga tgatcactgg tgttgagctt
     1921 tttttcatat gtttgttggc cgcataaatg tcttcttttg agaa
//
