LOCUS       NM_000799               1662 bp    mRNA    linear   PRI 11-SEP-2024
DEFINITION  Homo sapiens erythropoietin (EPO), mRNA.
ACCESSION   NM_000799
VERSION     NM_000799.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Maltaneri,R.E., Chamorro,M.E., Gionco,S.E., Nesse,A.B. and
            Vittori,D.C.
  TITLE     Erythropoietin enhances iron bioavailability in HepG2 cells by
            downregulating hepcidin through mTOR, C/EBPalpha and HIF-1alpha
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1871 (7), 119800 (2024)
   PUBMED   39047915
  REMARK    GeneRIF: Erythropoietin enhances iron bioavailability in HepG2
            cells by downregulating hepcidin through mTOR, C/EBPalpha and
            HIF-1alpha.
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Wu,G., Cao,B., Zhai,H., Liu,B., Huang,Y., Chen,X., Ling,H.,
            Ling,S., Jin,S., Yang,X. and Wang,J.
  TITLE     EPO promotes the progression of rheumatoid arthritis by inducing
            desialylation via increasing the expression of neuraminidase 3
  JOURNAL   Ann Rheum Dis 83 (5), 564-575 (2024)
   PUBMED   38272667
  REMARK    GeneRIF: EPO promotes the progression of rheumatoid arthritis by
            inducing desialylation via increasing the expression of
            neuraminidase 3.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1662)
  AUTHORS   Jin,X., Jin,W., Li,G., Zheng,J. and Xu,X.
  TITLE     Erythropoietin alleviates lung ischemia-reperfusion injury by
            activating the FGF23/FGFR4/ERK signaling pathway
  JOURNAL   PeerJ 12, e17123 (2024)
   PUBMED   38560469
  REMARK    GeneRIF: Erythropoietin alleviates lung ischemia-reperfusion injury
            by activating the FGF23/FGFR4/ERK signaling pathway.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1662)
  AUTHORS   Amalia,L. and Saputra,G.N.
  TITLE     Serum erythropoietin in acute ischemic stroke: preliminary findings
  JOURNAL   Sci Rep 14 (1), 2661 (2024)
   PUBMED   38302546
  REMARK    GeneRIF: Serum erythropoietin in acute ischemic stroke: preliminary
            findings.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1662)
  AUTHORS   Bunn,H.F.
  TITLE     Erythropoietin
  JOURNAL   Cold Spring Harb Perspect Med 3 (3), a011619 (2013)
   PUBMED   23457296
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1662)
  AUTHORS   Wenger,R.H. and Kurtz,A.
  TITLE     Erythropoietin
  JOURNAL   Compr Physiol 1 (4), 1759-1794 (2011)
   PUBMED   23733688
  REMARK    Review article
REFERENCE   7  (bases 1 to 1662)
  AUTHORS   Takeuchi,M. and Kobata,A.
  TITLE     Structures and functional roles of the sugar chains of human
            erythropoietins
  JOURNAL   Glycobiology 1 (4), 337-346 (1991)
   PUBMED   1820196
  REMARK    Review article
REFERENCE   8  (bases 1 to 1662)
  AUTHORS   Goldberg,M.A., Dunning,S.P. and Bunn,H.F.
  TITLE     Regulation of the erythropoietin gene: evidence that the oxygen
            sensor is a heme protein
  JOURNAL   Science 242 (4884), 1412-1415 (1988)
   PUBMED   2849206
REFERENCE   9  (bases 1 to 1662)
  AUTHORS   Sasaki,H., Ochi,N., Dell,A. and Fukuda,M.
  TITLE     Site-specific glycosylation of human recombinant erythropoietin:
            analysis of glycopeptides or peptides at each glycosylation site by
            fast atom bombardment mass spectrometry
  JOURNAL   Biochemistry 27 (23), 8618-8626 (1988)
   PUBMED   3219367
REFERENCE   10 (bases 1 to 1662)
  AUTHORS   Watkins,P.C., Eddy,R., Hoffman,N., Stanislovitis,P., Beck,A.K.,
            Galli,J., Vellucci,V., Gusella,J.F. and Shows,T.B.
  TITLE     Regional assignment of the erythropoietin gene to human chromosome
            region 7pter----q22
  JOURNAL   Cytogenet Cell Genet 42 (4), 214-218 (1986)
   PUBMED   2875851
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009488.5, X02157.1 and
            S65458.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000799.3.
            
            Summary: This gene encodes a secreted, glycosylated cytokine
            composed of four alpha helical bundles. The encoded protein is
            mainly synthesized in the kidney, secreted into the blood plasma,
            and binds to the erythropoietin receptor to promote red blood cell
            production, or erythropoiesis, in the bone marrow. Expression of
            this gene is upregulated under hypoxic conditions, in turn leading
            to increased erythropoiesis and enhanced oxygen-carrying capacity
            of the blood. Expression of this gene has also been observed in
            brain and in the eye, and elevated expression levels have been
            observed in diabetic retinopathy and ocular hypertension.
            Recombinant forms of the encoded protein exhibit neuroprotective
            activity against a variety of potential brain injuries, as well as
            antiapoptotic functions in several tissue types, and have been used
            in the treatment of anemia and to enhance the efficacy of cancer
            therapies. [provided by RefSeq, Aug 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X02157.1, BC093628.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2158188, SAMEA2159368
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000252723.3/ ENSP00000252723.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-332               AC009488.5         61278-61609
            333-929             X02157.1           1-597
            930-932             S65458.1           248-250
            933-1476            X02157.1           601-1144
            1477-1477           AC009488.5         64325-64325
            1478-1662           X02157.1           1146-1330
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q22.1"
     gene            1..1662
                     /gene="EPO"
                     /gene_synonym="DBAL; ECYT5; EP; MVCD2"
                     /note="erythropoietin"
                     /db_xref="GeneID:2056"
                     /db_xref="HGNC:HGNC:3415"
                     /db_xref="MIM:133170"
     exon            1..526
                     /gene="EPO"
                     /gene_synonym="DBAL; ECYT5; EP; MVCD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             514..1095
                     /gene="EPO"
                     /gene_synonym="DBAL; ECYT5; EP; MVCD2"
                     /note="epoetin"
                     /codon_start=1
                     /product="erythropoietin precursor"
                     /protein_id="NP_000790.2"
                     /db_xref="CCDS:CCDS5705.1"
                     /db_xref="GeneID:2056"
                     /db_xref="HGNC:HGNC:3415"
                     /db_xref="MIM:133170"
                     /translation="MGVHECPAWLWLLLSLLSLPLGLPVLGAPPRLICDSRVLERYLL
                     EAKEAENITTGCAEHCSLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVL
                     RGQALLVNSSQPWEPLQLHVDKAVSGLRSLTTLLRALGAQKEAISPPDAASAAPLRTI
                     TADTFRKLFRVYSNFLRGKLKLYTGEACRTGDR"
     exon            527..672
                     /gene="EPO"
                     /gene_synonym="DBAL; ECYT5; EP; MVCD2"
                     /inference="alignment:Splign:2.1.0"
     exon            673..759
                     /gene="EPO"
                     /gene_synonym="DBAL; ECYT5; EP; MVCD2"
                     /inference="alignment:Splign:2.1.0"
     exon            760..939
                     /gene="EPO"
                     /gene_synonym="DBAL; ECYT5; EP; MVCD2"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1662
                     /gene="EPO"
                     /gene_synonym="DBAL; ECYT5; EP; MVCD2"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1662
                     /gene="EPO"
                     /gene_synonym="DBAL; ECYT5; EP; MVCD2"
                     /note="major polyA site"
ORIGIN      
        1 cctttcccag atagcacgct ccgccagtcc caagggtgcg caaccggctg cactcccctc
       61 ccgcgaccca gggcccggga gcagccccca tgacccacac gcacgtctgc agcagccccg
      121 ctcacgcccc ggcgagcctc aacccaggcg tcctgcccct gctctgaccc cgggtggccc
      181 ctacccctgg cgacccctca cgcacacagc ctctccccca cccccacccg cgcacgcaca
      241 catgcagata acagccccga cccccggcca gagccgcaga gtccctgggc caccccggcc
      301 gctcgctgcg ctgcgccgca ccgcgctgtc ctcccggagc cggaccgggg ccaccgcgcc
      361 cgctctgctc cgacaccgcg ccccctggac agccgccctc tcctccaggc ccgtggggct
      421 ggccctgcac cgccgagctt cccgggatga gggcccccgg tgtggtcacc cggcgcgccc
      481 caggtcgctg agggaccccg gccaggcgcg gagatggggg tgcacgaatg tcctgcctgg
      541 ctgtggcttc tcctgtccct gctgtcgctc cctctgggcc tcccagtcct gggcgcccca
      601 ccacgcctca tctgtgacag ccgagtcctg gagaggtacc tcttggaggc caaggaggcc
      661 gagaatatca cgacgggctg tgctgaacac tgcagcttga atgagaatat cactgtccca
      721 gacaccaaag ttaatttcta tgcctggaag aggatggagg tcgggcagca ggccgtagaa
      781 gtctggcagg gcctggccct gctgtcggaa gctgtcctgc ggggccaggc cctgttggtc
      841 aactcttccc agccgtggga gcccctgcag ctgcatgtgg ataaagccgt cagtggcctt
      901 cgcagcctca ccactctgct tcgggctctg ggagcccaga aggaagccat ctcccctcca
      961 gatgcggcct cagctgctcc actccgaaca atcactgctg acactttccg caaactcttc
     1021 cgagtctact ccaatttcct ccggggaaag ctgaagctgt acacagggga ggcctgcagg
     1081 acaggggaca gatgaccagg tgtgtccacc tgggcatatc caccacctcc ctcaccaaca
     1141 ttgcttgtgc cacaccctcc cccgccactc ctgaaccccg tcgaggggct ctcagctcag
     1201 cgccagcctg tcccatggac actccagtgc cagcaatgac atctcagggg ccagaggaac
     1261 tgtccagaga gcaactctga gatctaagga tgtcacaggg ccaacttgag ggcccagagc
     1321 aggaagcatt cagagagcag ctttaaactc agggacagag ccatgctggg aagacgcctg
     1381 agctcactcg gcaccctgca aaatttgatg ccaggacacg ctttggaggc gatttacctg
     1441 ttttcgcacc taccatcagg gacaggatga cctggataac ttaggtggca agctgtgact
     1501 tctccaggtc tcacgggcat gggcactccc ttggtggcaa gagccccctt gacaccgggg
     1561 tggtgggaac catgaagaca ggatgggggc tggcctctgg ctctcatggg gtccaagttt
     1621 tgtgtattct tcaacctcat tgacaagaac tgaaaccacc aa
//
