LOCUS       NM_014964               4846 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens epsin 2 (EPN2), transcript variant 2, mRNA.
ACCESSION   NM_014964
VERSION     NM_014964.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Hsu,Y.H., Pintacuda,G., Liu,R., Nacu,E., Kim,A., Tsafou,K.,
            Petrossian,N., Crotty,W., Suh,J.M., Riseman,J., Martin,J.M.,
            Biagini,J.C., Mena,D., Ching,J.K.T., Malolepsza,E., Li,T.,
            Singh,T., Ge,T., Egri,S.B., Tanenbaum,B., Stanclift,C.R.,
            Apffel,A.M., Carr,S.A., Schenone,M., Jaffe,J., Fornelos,N.,
            Huang,H., Eggan,K.C. and Lage,K.
  CONSRTM   Schizophrenia Working Group of the Psychiatric Genomics Consortium;
            Stanley Global Asia Initiatives
  TITLE     Using brain cell-type-specific protein interactomes to interpret
            neurodevelopmental genetic signals in schizophrenia
  JOURNAL   iScience 26 (5), 106701 (2023)
   PUBMED   37207277
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 4846)
  AUTHORS   Sakai,E., Miura,Y., Suzuki-Kouyama,E., Oka,K., Tachibana,M.,
            Kawabata,K., Sakurai,F. and Mizuguchi,H.
  TITLE     A mammalian mirtron miR-1224 promotes tube-formation of human
            primary endothelial cells by targeting anti-angiogenic factor
            epsin2
  JOURNAL   Sci Rep 7 (1), 5541 (2017)
   PUBMED   28717225
  REMARK    GeneRIF: EPN2 mRNA is a direct target of miR-1224.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4846)
  AUTHORS   Chang,B., Tessneer,K.L., McManus,J., Liu,X., Hahn,S., Pasula,S.,
            Wu,H., Song,H., Chen,Y., Cai,X., Dong,Y., Brophy,M.L., Rahman,R.,
            Ma,J.X., Xia,L. and Chen,H.
  TITLE     Epsin is required for Dishevelled stability and Wnt signalling
            activation in colon cancer development
  JOURNAL   Nat Commun 6, 6380 (2015)
   PUBMED   25871009
  REMARK    GeneRIF: Epsins are required for Dishevelled stability and Wnt
            signalling activation in colon cancer development.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4846)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4846)
  AUTHORS   Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
            Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
  TITLE     Functional proteomics mapping of a human signaling pathway
  JOURNAL   Genome Res 14 (7), 1324-1332 (2004)
   PUBMED   15231748
REFERENCE   7  (bases 1 to 4846)
  AUTHORS   Hussain,N.K., Yamabhai,M., Bhakar,A.L., Metzler,M., Ferguson,S.S.,
            Hayden,M.R., McPherson,P.S. and Kay,B.K.
  TITLE     A role for epsin N-terminal homology/AP180 N-terminal homology
            (ENTH/ANTH) domains in tubulin binding
  JOURNAL   J Biol Chem 278 (31), 28823-28830 (2003)
   PUBMED   12750376
REFERENCE   8  (bases 1 to 4846)
  AUTHORS   Ford,M.G., Mills,I.G., Peter,B.J., Vallis,Y., Praefcke,G.J.,
            Evans,P.R. and McMahon,H.T.
  TITLE     Curvature of clathrin-coated pits driven by epsin
  JOURNAL   Nature 419 (6905), 361-366 (2002)
   PUBMED   12353027
REFERENCE   9  (bases 1 to 4846)
  AUTHORS   Rosenthal,J.A., Chen,H., Slepnev,V.I., Pellegrini,L., Salcini,A.E.,
            Di Fiore,P.P. and De Camilli,P.
  TITLE     The epsins define a family of proteins that interact with
            components of the clathrin coat and contain a new protein module
  JOURNAL   J Biol Chem 274 (48), 33959-33965 (1999)
   PUBMED   10567358
REFERENCE   10 (bases 1 to 4846)
  AUTHORS   Salcini,A.E., Confalonieri,S., Doria,M., Santolini,E., Tassi,E.,
            Minenkova,O., Cesareni,G., Pelicci,P.G. and Di Fiore,P.P.
  TITLE     Binding specificity and in vivo targets of the EH domain, a novel
            protein-protein interaction module
  JOURNAL   Genes Dev 11 (17), 2239-2249 (1997)
   PUBMED   9303539
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC106017.11 and AC124066.6.
            
            On Apr 8, 2021 this sequence version replaced NM_014964.4.
            
            Summary: This gene encodes a protein which interacts with clathrin
            and adaptor-related protein complex 2, alpha 1 subunit. The protein
            is found in a brain-derived clathrin-coated vesicle fraction and
            localizes to the peri-Golgi region and the cell periphery. The
            protein is thought to be involved in clathrin-mediated endocytosis.
            Alternate splicing of this gene results in multiple transcript
            variants encoding different isoforms. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (2) represents the longest
            transcript and encodes the longest isoform (b).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB028988.1, SRR14038194.1156161.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000314728.10/ ENSP00000320543.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-166               AC106017.11        127257-127422
            167-289             AC106017.11        171846-171968
            290-1054            AC106017.11        172841-173605
            1055-1225           AC106017.11        175511-175681
            1226-1338           AC124066.6         86784-86896         c
            1339-1431           AC124066.6         84637-84729         c
            1432-1606           AC124066.6         83502-83676         c
            1607-1783           AC124066.6         67894-68070         c
            1784-1870           AC124066.6         67134-67220         c
            1871-2086           AC124066.6         64713-64928         c
            2087-4846           AC124066.6         60066-62825         c
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p11.2"
     gene            1..4846
                     /gene="EPN2"
                     /gene_synonym="EHB21"
                     /note="epsin 2"
                     /db_xref="GeneID:22905"
                     /db_xref="HGNC:HGNC:18639"
                     /db_xref="MIM:607263"
     exon            1..166
                     /gene="EPN2"
                     /gene_synonym="EHB21"
                     /inference="alignment:Splign:2.1.0"
     exon            167..289
                     /gene="EPN2"
                     /gene_synonym="EHB21"
                     /inference="alignment:Splign:2.1.0"
     exon            290..1054
                     /gene="EPN2"
                     /gene_synonym="EHB21"
                     /inference="alignment:Splign:2.1.0"
     CDS             460..2385
                     /gene="EPN2"
                     /gene_synonym="EHB21"
                     /note="isoform b is encoded by transcript variant 2; Eps15
                     binding protein; EPS-15-interacting protein 2"
                     /codon_start=1
                     /product="epsin-2 isoform b"
                     /protein_id="NP_055779.2"
                     /db_xref="CCDS:CCDS11203.1"
                     /db_xref="GeneID:22905"
                     /db_xref="HGNC:HGNC:18639"
                     /db_xref="MIM:607263"
                     /translation="MTTSSIRRQMKNIVNNYSEAEIKVREATSNDPWGPSSSLMTEIA
                     DLTYNVVAFSEIMSMVWKRLNDHGKNWRHVYKALTLLDYLIKTGSERVAQQCRENIFA
                     IQTLKDFQYIDRDGKDQGINVREKSKQLVALLKDEERLKAERAQALKTKERMAQVATG
                     MGSNQITFGRGSSQPNLSTSHSEQEYGKAGGSPASYHGSPEASLCPQHRTGAPLGQSE
                     ELQPLSQRHPFLPHLGLASRPNGDWSQPCLTCDRAARATSPRVSSELEQARPQTSGEE
                     ELQLQLALAMSREVAEQEERLRRGDDLRLQMALEESRRDTVKIPKKKEHGSLPQQTTL
                     LDLMDALPSSGPAAQKAEPWGPSASTNQTNPWGGPAAPASTSDPWPSFGTKPAASIDP
                     WGVPTGATVQSVPKNSDPWAASQQPASSAGKRASDAWGAVSTTKPVSVSGSFELFSNL
                     NGTIKDDFSEFDNLRTSKKTAESVTSLPSQNNGTTSPDPFESQPLTVASSKPSSARKT
                     PESFLGPNAALVNLDSLVTRPAPPAQSLNPFLAPGAPATSAPVNPFQVNQPQPLTLNQ
                     LRGSPVLGTSTSFGPGPGVESMAVASMTSAAPQPALGATGSSLTPLGPAMMNMVGSVG
                     IPPSAAQATGTTNPFLL"
     exon            1055..1225
                     /gene="EPN2"
                     /gene_synonym="EHB21"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1338
                     /gene="EPN2"
                     /gene_synonym="EHB21"
                     /inference="alignment:Splign:2.1.0"
     exon            1339..1431
                     /gene="EPN2"
                     /gene_synonym="EHB21"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..1606
                     /gene="EPN2"
                     /gene_synonym="EHB21"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1783
                     /gene="EPN2"
                     /gene_synonym="EHB21"
                     /inference="alignment:Splign:2.1.0"
     exon            1784..1870
                     /gene="EPN2"
                     /gene_synonym="EHB21"
                     /inference="alignment:Splign:2.1.0"
     exon            1871..2086
                     /gene="EPN2"
                     /gene_synonym="EHB21"
                     /inference="alignment:Splign:2.1.0"
     exon            2087..4846
                     /gene="EPN2"
                     /gene_synonym="EHB21"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4821..4826
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPN2"
                     /gene_synonym="EHB21"
                     /note="hexamer: AATATA"
     polyA_site      4846
                     /gene="EPN2"
                     /gene_synonym="EHB21"
                     /note="major polyA site"
ORIGIN      
        1 acgcgtccgg cgcgccccct cccggccgcc atgttggctg gtgtgtgggt gtcaaactga
       61 gccagacgcg gcggtggcgg cggctccgcg ggctacggtc gctcccgcct ctcgagcgct
      121 gccggtggcc gcagcggcgc acccacgccg gcccggagga gcagagtgtt catttctgtg
      181 tcgggcacag tgctaagtgc tgggtgctca ctggtgatga ggcagatgaa ggttaccaaa
      241 cttgtggaca ggagcctcat atcagagacg tggacctcac tgtagcctgg tcatggcttc
      301 cagcttttcg aatctgaggc tccaaaggag gaaatgacca ttcagggatc ttactccagc
      361 ttgattacgg agactgaacc ttcatagggt gcgcacttac caaggacagg aaggtttctc
      421 tgtttgaagg gctttaaact tataacaaag aaaataaaaa tgacgacttc gtctatcaga
      481 cggcagatga aaaacatcgt gaacaattac tcagaggcag aaatcaaagt ccgggaagcc
      541 acctccaatg acccgtgggg cccgtccagt tctctgatga ccgagattgc cgacctgacc
      601 tacaacgtgg tggccttctc ggagatcatg agcatggtgt ggaagcggct gaatgaccat
      661 ggcaagaact ggcggcatgt gtacaaggcg ctgaccctgc tggactacct catcaagaca
      721 ggctccgaac gtgtggccca gcagtgccgg gagaacatct tcgccatcca gaccctgaag
      781 gacttccagt acattgaccg agatggcaag gaccagggca tcaatgtgcg tgagaagtca
      841 aagcaactgg tggctctcct caaggacgag gaacggttga aggctgagag ggcccaggct
      901 ctcaaaacca aagagcgcat ggcccaggtt gccactggca tgggcagcaa ccagatcacc
      961 tttgggcgag gctccagcca gcccaacctc tccaccagcc actcggagca ggagtatggc
     1021 aaggccgggg gctccccggc ctcctaccat ggctcgcctg aggcctcgct gtgcccccag
     1081 caccgcacag gggccccgct gggtcagagt gaggagctgc agccactgag ccagcgccac
     1141 cccttcctgc cgcacctggg gctggcctcc cgcccaaatg gcgactggtc ccagccctgc
     1201 ctcacttgtg accgcgcagc ccgagccacc tccccgcgag tgtcctccga gctggagcaa
     1261 gcccggcccc agactagtgg agaagaggag cttcagctgc agctggcact tgccatgagc
     1321 agagaagtgg ctgagcagga agaacgcctc aggcggggtg atgacctcag attacagatg
     1381 gccctggaag aaagccgaag ggacacagtt aaaattccaa aaaagaaaga gcatggctct
     1441 ctcccacagc agactacgct gttggattta atggatgctc tccccagctc gggccccgcg
     1501 gcccagaaag cagagccctg gggcccgtca gcctccacta accagaccaa cccctggggc
     1561 gggccagcgg ctcctgcgag tacttcagac ccctggccat cgtttggtac caagccagct
     1621 gcctccattg acccatgggg ggtgcccact ggagccaccg tacaatctgt ccccaagaac
     1681 tcggacccct gggcagcttc acagcagcct gcctccagtg ctgggaaaag agcttctgac
     1741 gcgtggggcg cagtctccac caccaagccc gtgtctgtct ctgggtcctt tgagctcttc
     1801 agtaatctga atggtacaat taaagatgac ttttctgaat ttgacaacct tcggacttca
     1861 aaaaaaacag ccgaatctgt gacctctctg ccatcccaaa acaatggaac taccagccct
     1921 gacccctttg agtctcaacc cctgactgtc gcctcaagca agcccagcag tgcccggaaa
     1981 acacctgagt ccttcctggg ccccaacgcg gccctggtga acctggactc actggtgacc
     2041 aggcctgccc caccagccca gtccctcaac cctttcctgg caccaggtgc tcccgccacc
     2101 tcggcccctg ttaacccttt ccaggtgaac cagccccagc cgctgacact gaaccagctt
     2161 cgggggagcc cagtcctggg gaccagcaca tcctttgggc ctggcccagg agtggagtcc
     2221 atggctgtgg cctcgatgac ctccgcggcc ccacagccag ctctgggggc cactggttcc
     2281 tctctgacac cactgggccc tgcaatgatg aacatggtgg gcagtgtggg tataccccca
     2341 tcagcagccc aggccactgg cacaaccaac cctttccttc tctagtgcct gggcctggga
     2401 cccacccaga gcacctgtgc tggaggatgc cgagcaggga ctctcgtctg tgggacggga
     2461 tccaagagtt tggggattag gggtattagg gcttttcagc tccagcttcc tgatgaaagg
     2521 gctgtcttta cagccccaac cctcagaccc tcgccttcca aggcaggccc ctcagcctgg
     2581 cctgctctca ccacctcctc caaagcactg aggtcctggc agggctcctc tgaggccttg
     2641 gacgaggacg tggagtcatc agtgttgccc ttgcccctaa cctcagcccg agggtctcca
     2701 ggatgttgcc tggcccagga cttgggacag tggccttgtc tttgtcctcc ccacccccca
     2761 gccctaggga caccccaggc agtcctgggt gtggacacga tgaagcaccg gctccataag
     2821 acaccttttg gggaagtggt tgtgcatatt ttatttttct ttgactcgtg tgagttcaaa
     2881 gtaaacacca ccaccgtgga caactcttga attaaatcca ctagagcgag ctttaaaact
     2941 aatctgagca taacccccaa cgtggctcta tgcttgtgta cctttgcaca tattttgttt
     3001 agtacagttt catatttgag tttgcagaat tatctaatag tctttttttg gctaatattt
     3061 ttataacgtg gttcttattt aactgtctag ttttgataga atttaccagg tctggctgaa
     3121 tgaagatgtt ggcacgtgtc attttagtgg tttaaatcct cttatttatg gttttaactc
     3181 taagaaaatt ttaaaaggaa gagatgtttg gatgacaaaa aaatgcctta ttgaaaaact
     3241 aaagcaaatt ctttatagga agaaaatatg ggaatttgat tacacataga tgatgatgtt
     3301 tatttaaaaa aaaaacaaca gcaacaaaaa atcctagcct ccagttgcct tttcctttct
     3361 ttagctcctg tttttggtga attactaaac tgattgactt tcagcctttt tggctagatc
     3421 ctgagaggct atttttctta cgaatatacc aacatcctga aagttaaaga aaaaaatcta
     3481 atgtatgaat gtgactcacc aatttttatc aactaattcc ttttttttat taaaggcatg
     3541 cagggattaa caggacttct gtttacaatg gaaatctgaa atggaagaaa catctttaac
     3601 cttgtgtgtc tgtgatctcc tctgtcttaa tccacgctca ggctaaagat ggggataatg
     3661 tggaaatggc agttgtcccg agggcgtggg gtggggggtg cttctgtgcc cacgggtccc
     3721 tgggcaacag tccctaggct aagacagggg tggggggcta agggaccagg gctggccctg
     3781 atccacctac ctgctaactc cagatattat ttttaagttg gagacctaaa aataattctc
     3841 ttgtattttg gagatgaaga aaaaagtcat tcacctggga gggattttta acaaacatgc
     3901 actaatatta cctccctctt ccaaactcct actgtcctca cctcacccac cactgtaaaa
     3961 gtattacgtc gtgcattaaa gaccagaaaa gacttgcttt tacccaggga ggggacattc
     4021 ccccaatcct gtgtctgggc agcttgcctg gtggatgttt ctggcccttt ttccaccatg
     4081 ggaatttctc acctctagct ctaagggcag ctgaccacct gccagcccct ctctggttcc
     4141 agaagattac ccttcgcacc ggcacaggag agatcttaaa tggtctctgg gctgctggga
     4201 catgcaggct ctgagtgggg actgcaccca ctcatgctgt tcctgaggcc gccctctcca
     4261 gtcccaggag ccgtgtctag agttcctgac cagccacctt ctgccagaac tgcaggcctg
     4321 cggctgggtc ttaggctccc gctgccattt gggtaagccg gtggctggtc tcgtctgccg
     4381 ggggaagggt ggggaggtgc aatgggatgg gaggcaggat gcttgcccag aaaggtgctt
     4441 tccttcagac ggtgcagggc ctgggccagc cttacaggtc agtagactag actcgactac
     4501 ttggggtcag tgattctttt gtaacgagtc tttcatgatg tgactttgag gccccaacat
     4561 gacagccact gggccaccgg gacccaagaa gacagccccc tgccccatct tctggcacag
     4621 gccatcccga gtgcatgtcc ctgttgccca ctgcactgat catgaagcca ccggccactg
     4681 ccacgcatgt tgcacctgtg ccatcgtgct ccctcctgat gggcaccgtg gtggaggaag
     4741 tcacccagct gtttctcagt cccagaggcc ggtggctggt tttgaacttg tgttgactgt
     4801 tgatacttat ttactgtata aatataattt atcatttgta ccatga
//
