LOCUS       NM_001130072           16219 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens epsin 1 (EPN1), transcript variant 2, mRNA.
ACCESSION   NM_001130072
VERSION     NM_001130072.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 16219)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 16219)
  AUTHORS   Tsunoda,T., Riku,M., Yamada,N., Tsuchiya,H., Tomita,T., Suzuki,M.,
            Kizuki,M., Inoko,A., Ito,H., Murotani,K., Murakami,H., Saeki,Y. and
            Kasai,K.
  TITLE     ENTREP/FAM189A2 encodes a new ITCH ubiquitin ligase activator that
            is downregulated in breast cancer
  JOURNAL   EMBO Rep 23 (2), e51182 (2022)
   PUBMED   34927784
REFERENCE   3  (bases 1 to 16219)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   4  (bases 1 to 16219)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 16219)
  AUTHORS   Kroppen,B., Teske,N., Yambire,K.F., Denkert,N., Mukherjee,I.,
            Tarasenko,D., Jaipuria,G., Zweckstetter,M., Milosevic,I.,
            Steinem,C. and Meinecke,M.
  TITLE     Cooperativity of membrane-protein and protein-protein interactions
            control membrane remodeling by epsin 1 and affects
            clathrin-mediated endocytosis
  JOURNAL   Cell Mol Life Sci 78 (5), 2355-2370 (2021)
   PUBMED   32997199
  REMARK    GeneRIF: Cooperativity of membrane-protein and protein-protein
            interactions control membrane remodeling by epsin 1 and affects
            clathrin-mediated endocytosis.
REFERENCE   6  (bases 1 to 16219)
  AUTHORS   Drake,M.T., Downs,M.A. and Traub,L.M.
  TITLE     Epsin binds to clathrin by associating directly with the
            clathrin-terminal domain. Evidence for cooperative binding through
            two discrete sites
  JOURNAL   J Biol Chem 275 (9), 6479-6489 (2000)
   PUBMED   10692452
REFERENCE   7  (bases 1 to 16219)
  AUTHORS   Rosenthal,J.A., Chen,H., Slepnev,V.I., Pellegrini,L., Salcini,A.E.,
            Di Fiore,P.P. and De Camilli,P.
  TITLE     The epsins define a family of proteins that interact with
            components of the clathrin coat and contain a new protein module
  JOURNAL   J Biol Chem 274 (48), 33959-33965 (1999)
   PUBMED   10567358
REFERENCE   8  (bases 1 to 16219)
  AUTHORS   Morinaka,K., Koyama,S., Nakashima,S., Hinoi,T., Okawa,K.,
            Iwamatsu,A. and Kikuchi,A.
  TITLE     Epsin binds to the EH domain of POB1 and regulates
            receptor-mediated endocytosis
  JOURNAL   Oncogene 18 (43), 5915-5922 (1999)
   PUBMED   10557078
REFERENCE   9  (bases 1 to 16219)
  AUTHORS   Chen,H., Fre,S., Slepnev,V.I., Capua,M.R., Takei,K., Butler,M.H.,
            Di Fiore,P.P. and De Camilli,P.
  TITLE     Epsin is an EH-domain-binding protein implicated in
            clathrin-mediated endocytosis
  JOURNAL   Nature 394 (6695), 793-797 (1998)
   PUBMED   9723620
REFERENCE   10 (bases 1 to 16219)
  AUTHORS   Ikeda,M., Ishida,O., Hinoi,T., Kishida,S. and Kikuchi,A.
  TITLE     Identification and characterization of a novel protein interacting
            with Ral-binding protein 1, a putative effector protein of Ral
  JOURNAL   J Biol Chem 273 (2), 814-821 (1998)
   PUBMED   9422736
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008735.9, AC010525.6 and
            AC008749.6.
            
            On Aug 2, 2019 this sequence version replaced NM_001130072.1.
            
            Summary: This gene encodes a member of the epsin protein family.
            The encoded protein binds clathrin and is involved in the
            endocytosis of clathrin-coated vesicles. Loss of function of this
            gene is associated with reduced tumor growth and progression in
            certain cancer types. [provided by RefSeq, Mar 2016].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK022454.1, SRR7346977.2777081.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000270460.11/ ENSP00000270460.6
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-210               AC008735.9         912-1121            c
            211-539             AC010525.6         7809-8137
            540-789             AC010525.6         14678-14927
            790-914             AC010525.6         18152-18276
            915-989             AC010525.6         18579-18653
            990-1073            AC010525.6         19149-19232
            1074-1377           AC010525.6         21036-21339
            1378-1488           AC010525.6         21968-22078
            1489-1575           AC010525.6         22233-22319
            1576-1833           AC010525.6         24008-24265
            1834-14210          AC010525.6         24430-36806
            14211-16219         AC008749.6         13623-15631
FEATURES             Location/Qualifiers
     source          1..16219
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.42"
     gene            1..16219
                     /gene="EPN1"
                     /note="epsin 1"
                     /db_xref="GeneID:29924"
                     /db_xref="HGNC:HGNC:21604"
                     /db_xref="MIM:607262"
     exon            1..210
                     /gene="EPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            211..539
                     /gene="EPN1"
                     /inference="alignment:Splign:2.1.0"
     CDS             312..2042
                     /gene="EPN1"
                     /note="isoform b is encoded by transcript variant 2; EH
                     domain-binding mitotic phosphoprotein; EPS-15-interacting
                     protein 1"
                     /codon_start=1
                     /product="epsin-1 isoform b"
                     /protein_id="NP_001123544.1"
                     /db_xref="CCDS:CCDS46199.1"
                     /db_xref="GeneID:29924"
                     /db_xref="HGNC:HGNC:21604"
                     /db_xref="MIM:607262"
                     /translation="MSTSSLRRQMKNIVHNYSEAEIKVREATSNDPWGPSSSLMSEIA
                     DLTYNVVAFSEIMSMIWKRLNDHGKNWRHVYKAMTLMEYLIKTGSERVSQQCKENMYA
                     VQTLKDFQYVDRDGKDQGVNVREKAKQLVALLRDEDRLREERAHALKTKEKLAQTATA
                     SSAAVGSGPPPEAEQAWPQSSGEEELQLQLALAMSKEEADQPPSCGPEDDAQLQLALS
                     LSREEHDKEERIRRGDDLRLQMAIEESKRETGGKEESSLMDLADVFTAPAPAPTTDPW
                     GGPAPMAAAVPTAAPTSDPWGGPPVPPAADPWGGPAPTPASGDPWRPAAPAGPSVDPW
                     GGTPAPAAGEGPTPDPWGSSDGGVPVSGPSASDPWTPAPAFSDPWGGSPAKPSTNGTT
                     AAGGFDTEPDEFSDFDRLRTALPTSGSSAGELELLAGEVPARSPGAFDMSGVRGSLAE
                     AVGSPPPAATPTPTPPTRKTPESFLGPNAALVDLDSLVSRPGPTPPGAKASNPFLPGG
                     GPATGPSVTNPFQPAPPATLTLNQLRLSPVPPVPGAPPTYISPLGGGPGLPPMMPPGP
                     PAPNTNPFLL"
     exon            540..789
                     /gene="EPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            790..914
                     /gene="EPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            915..989
                     /gene="EPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1073
                     /gene="EPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1377
                     /gene="EPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1488
                     /gene="EPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1489..1575
                     /gene="EPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..1833
                     /gene="EPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..16219
                     /gene="EPN1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2425..2430
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPN1"
                     /note="hexamer: AATAAA"
     polyA_site      2446
                     /gene="EPN1"
                     /note="major polyA site"
     regulatory      12377..12382
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPN1"
                     /note="hexamer: AATAAA"
     polyA_site      12400
                     /gene="EPN1"
     regulatory      16194..16199
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPN1"
                     /note="hexamer: ATTAAA"
     polyA_site      16219
                     /gene="EPN1"
ORIGIN      
        1 ctgttgcttc ccgtctcctc ggcggctccc ctcccccgcc cggctctccg cgccccttct
       61 gggcggcggg gcggcggagc cgtcggcgtg cggccctcct tgcgttcgtg cgtgcgcccg
      121 tggcccggcg cacgtcccgc gacaccgagg ccgagcgggg cagggggctg accgccatga
      181 ccccccagag cccggcgtga gggggccgag atgcggtgac ctgccagcac ctgccgcagc
      241 cttcgtccgg gagtcgcccc atctctccac gcatcggggc cctgtgcccc ttgctgctgc
      301 agccgggcac catgtcgacc tcgtccttga ggcgccagat gaagaacatc gtccacaact
      361 actcagaggc ggagatcaag gttcgagagg ccacgagcaa tgacccctgg ggcccatcca
      421 gctccctcat gtcagagatt gccgacctca cctacaacgt tgtcgccttc tcggagatca
      481 tgagcatgat ctggaagcgg ctcaatgacc atggcaagaa ctggcgtcac gtttacaagg
      541 ccatgacgct gatggagtac ctcatcaaga ccggctcgga gcgcgtgtcg cagcagtgca
      601 aggagaacat gtacgccgtg cagacgctga aggacttcca gtacgtggac cgcgacggca
      661 aggaccaggg cgtgaacgtg cgtgagaaag ctaagcagct ggtggccctg ctgcgcgacg
      721 aggaccggct gcgggaagag cgggcgcacg cgctcaagac caaggaaaag ctggcacaga
      781 ccgccacggc ctcatcagca gctgtgggct caggcccccc tcccgaggcg gagcaggcgt
      841 ggccgcagag cagcggggag gaggagctgc agctccagct ggccctggcc atgagcaagg
      901 aggaggccga ccagcccccg tcctgcggcc ccgaggacga cgcccagctc cagctggccc
      961 ttagtttgag ccgagaagag catgataagg aggagcggat ccgtcgcggg gatgacctgc
     1021 ggctgcagat ggcaatcgag gagagcaaga gggagactgg gggcaaggag gagtcgtccc
     1081 tcatggacct tgctgacgtc ttcacggccc cagctcctgc cccgaccaca gacccctggg
     1141 ggggcccagc acccatggct gctgccgtcc ccacggctgc ccccacctcg gacccctggg
     1201 gcggcccccc tgtccctcca gctgctgatc cctggggagg tccagccccc acgccggcct
     1261 ctggggaccc ctggaggcct gctgcccctg caggaccctc agttgaccct tggggtggga
     1321 ccccagcccc tgcagctggg gaggggccca cgcctgatcc atggggaagt tccgatggtg
     1381 gggtcccggt cagtgggccc tcagcctccg atccctggac accggccccg gccttctcag
     1441 atccctgggg agggtcacct gccaagccca gcaccaatgg cacaacagca gccgggggat
     1501 tcgacacgga gcccgacgag ttctctgact ttgaccgact ccgcacggca ctgccgacct
     1561 ccgggagcag cgcaggagag ctggagctgc tggcaggaga ggtgccggcc cgaagccctg
     1621 gggcgtttga catgagtggg gtcaggggat ctctggctga ggctgtgggc agccccccac
     1681 ctgcagccac accaactccc acgcccccca cccggaagac gccggagtca ttcctggggc
     1741 ccaatgcagc cctcgtcgac ctggactcgc tggtgagccg gccgggcccc acgccgcctg
     1801 gagccaaggc ctccaacccc ttcctgccag gcggaggccc agccactggc ccttccgtca
     1861 ccaacccctt ccagcccgcg cctcccgcga cgctcaccct gaaccagctc cgtctcagtc
     1921 ctgtgcctcc cgtccctgga gcgccaccca cgtacatctc tccccttggc gggggccctg
     1981 gcctgccccc catgatgccc ccgggccccc cggcccccaa cactaatccc ttcctcctat
     2041 aatccagggc ggaagggggc ctggctccat ccggctgccc cattccggct ccctgggaga
     2101 tcagtgttgt gagtgcatgt gaaatggggg atccccaccc ccagtgccct tccccttcct
     2161 ggggcccact cacactacac cctcttcctt tcccacccca cctccccgga gagaaactgg
     2221 acatggggcc tggggagggg agctggccag aggaggaccc ctttcccgtg gcattagaag
     2281 ggggaggggt ggctggggcc cccacccatt ccccctccct ccaaactccc aacccccagt
     2341 cagtgtttga gcctcctcgt tcccctcacg cacccgctca cgcaccctcg gtgaatcctt
     2401 ggtgatgatt ttggcaactt tgggaataaa tggcaattcc cacgggcttg gcactcccag
     2461 attcccatct gtgatttcgt gtgtccccca gagccccttc atccagggac ccagggctca
     2521 ggtcacatct ctttctcggg ggtggctggc aaccagcccc tctgtctcag aagccctgat
     2581 ttccctgacc ctcccaggtc aagggatggc tgggttgagg gtggactgtg cgaggcctgg
     2641 cggagctgta gcccacacag gtccaggtgg tcgggaggcc actcatggcc agacgcttcc
     2701 ggtgcaggag gccttgctct tgtgttgagg ggacaccagg atgaggtcag agagtgcact
     2761 ggggttcagg gaggagaaga atcctgagag atggcacatg tgcaacacag gtcaccgtcg
     2821 ccatcgcccg ggcccaccgc aagcatcact aatccatccc tgcactcctg gaaagccggg
     2881 tcagcctctg ccggctgagc tgagtggacc accaggcgcc tcctggttgt cctctgtgtg
     2941 ctgttttcct ctgtgtgctc ttgtcctggg ctggaccaca cctggtgaga ctatccggga
     3001 gaatcctcgg gccgccttgt gaggggggtg ttgctgtcat ctccagggct aggtgacagc
     3061 ttgttgcagg ccccacagcc agggcctcag ggatggggat ctggccatgt ctgttttaag
     3121 ccgaacaggc tgttcttcca tgtggagtct ccctccgtga tcccctgggg tcaagtcctg
     3181 acggctgggt tcgctccctc agtgactccg acagctcttg tctcagaggt gctggggtga
     3241 aggctgtgtg gggccaggcc cctggggtca gacttcttgc cccttctgcc cgggggacgc
     3301 tctgggctca gggtggggcc agacagctac atcacaggga cacttgttat gggtcggggt
     3361 ccagggagca cccagccagt ggagtgttgg aggccactga gagggacatg actgcggtgt
     3421 ccgagcagcg tcgtggccac cctgtgtgcc cactccctgg tggacagacc tacgtcacct
     3481 ggggattgca gagcaaaagg agtgctgttg gggtgatgtg aggttggggt tctgtttctt
     3541 gctgtgaatt gggtcatgac ctgtgtcgga gcgttctctg gcctccactt gcccctgcag
     3601 accagggtgt ctggagggtt gtcgggggct tgatggagga attgctgacc acaccatgca
     3661 ggaagaggtg ggtcccaggg acacaggaac tttggggtca gccggttcca gattcagttg
     3721 cagaggagca tcccccttgc ttagccaggg aggggaagcc ctcttctcac acccggcctg
     3781 ggtatgttgt gggagagtca tttcccagga ggaaatgggg actgttaccg ggggaggggg
     3841 tgctgtcccc aggcagcagc aagcgtccct tacgcttggg ctgtccgaga ccctgctgct
     3901 ctaggcggtt gtgatggggg agccccacag ggcacagaga gccagctgaa ggcggcagct
     3961 ctgggtggga tcacatggaa aggggaggcg agccgagcct ccctcttgcc aatcttggtc
     4021 tccccacagg gtcatagctc ctgggcccct cacttgtcag accctccatt caccaaatta
     4081 aggataagtt tcacgatttc gcactgcgga gcctgcactc cctttctctt caaaaaagct
     4141 gaggacgaag ataaaatgat acagttggat tagtccagtg gaggggttaa aggtgtgagg
     4201 ctttaattct ccccaccatt tcctcatttg gcaaaacaga gaagacagga ttgaaaaaca
     4261 agacaaaaac ccagaggctt gcacataaat ttggggctta actacagcat cttcggcatc
     4321 ttcttttccc tacagtgcga attacaaagt agcaccacag atctcgtgtc tcctacttta
     4381 ttcctctttc tagaatattc cagccaccaa agtgctcagc aggagctgct gtgtaggagt
     4441 cccccatgag gcccaagcac ctgagcttag tgacatacac ggttgaacgc tgggagaatg
     4501 ctgggagcct gctgttgtcc ccgattgagg gctcccccca ctgtgtgtgt atcactcttt
     4561 ctacagcaga tgtgtctctc tccccacagt gggatggcga gcttgttaaa tggccgtgag
     4621 gaagacttta ttcaggacca tcgagacagg tacaggtacc atcagtggat ggaaaatcac
     4681 taagagcaaa cctcaggggt ccgggggttt tcggctaaac ccacctagca ggattcttgc
     4741 tgaaggcagg ccagggtgat cagaggtcac ctggggatgg tggaggctga agaagcctat
     4801 cagatgtgag ggtggcgggg gttggggggg atggcggcgg ggttcatttg ctgaaaggat
     4861 tttacaaggg catgcacgga tgggcctagg aggaggttca gatccctcag gtttggctaa
     4921 gcaagagtca tggtggtgct cccggctggc tctcaggagc agatgtgctt ctgacccgac
     4981 gtcttccccc agagagggct gaagtggaaa aaaccccaga atttctccct gggacaggtg
     5041 aagcgatggg gcgtgggaca ttcctgggtc tcgactctgt tcagggccta ggggttgggt
     5101 ggggaaggca cttgagggaa ccttcctgga gcccgtagga gcttcatccg agctcctggg
     5161 tcaccccgcc caccagagtc caggcccacc agagtcccct gccacctgtg ctcaccctag
     5221 ccttacccct cgctctactc ccgccctcgc cctatcctcg ctcctcgccc tacgcttgcc
     5281 cctcgctctg gagctgcgat gccagcctca gggctttcag tcctgccttg atcttttttt
     5341 atactgaaac tcaattttac acacacaaac ccactcccag ccagcctctg ccttttgcct
     5401 tttcctcatg ttctcattga ataggaagaa atgccctttt ttttcccccc tagaaggagt
     5461 cttgctctgt tgccaggctg gagtgcagtg gcacgatctc ggctcaccgc aacctccacc
     5521 tcccgggttc aagcgattct cctgcctcag cctctcgagt agctgggatt acaggtgccc
     5581 accaccacgc ccagctaatt tttttttgta tttttagtac agacggggtt tcaccatgtt
     5641 aggatggtct cgatctcctg acctcgtgat ccgcctgcct cggcctccca aagtgctggg
     5701 attacaggcg tgagccactg tagcggcccc caccccattt cattttttat ttttttatta
     5761 tactttaagt tctagggtac ctatgcacaa cgcgcaggtt tgttacatat atatacatgt
     5821 gccatgttgg tgtgctgcac gcattaactc gtcatttaca ttaggtatat ctcctaatgc
     5881 cttccctccc gcctccccca ggcccttagt ttgtgctgct ctgcgttggt tttctgcgtg
     5941 tgctaaaagg ggagggagag tttctgtgcc cattcccagg caccttcttg cagccgcagg
     6001 catctccccc acacgtgctt ctagcttccc gggtgtggcg tgagccattg cgtccggcca
     6061 gaaatgtttt atttcttgag aacttgtctg tctctgtgat gactgtgggt tgtccagctc
     6121 atccctcaag cgccatcctg ccaggccctt cactgtggtg agaaaccggc ctactcacat
     6181 cacaggtgcc cctggagttt ctcaaactgc agcccaagga ttggctgctt gcttttgtaa
     6241 aaaaaaatgt tttattagag tgcagccacg gctgcttctg cactacagtg gcaatggtga
     6301 cattgagtgt ggcagagacc gagtgtccct tgagcctaaa atatttacta tctggctctt
     6361 caacaaaaag tgtgctgacc cccggtgatt gcttgtatgt ggcaggggtc taaggcctgc
     6421 ctgcacgtcc tgggacagcc cacgcttcca cagggttcca gcatgctttc cgccgagaaa
     6481 tcgctccctt tgctgtttgt tttgtattga atgtttcttc atgcttttgt tttactttca
     6541 gggataatta tataatgtta tgtttcatcc acattatttt agtatggata tttataattt
     6601 cctaaagcac tatttttttt ttttgagaca gagtcttgct ctgtcgccca ggctggagtg
     6661 cagtggctcg atctctgctc actgcaagct ccgcctcctg tgttcacgcc attctcctgc
     6721 ctcagcctcc caagtagctg ggactacaga tgcccaccac catgcccagc taattttttt
     6781 gtattttttt agtagagacg gggtttcact gtgttagcca ggatggtcac gatctcctga
     6841 cctcgtgatc tgcccacctc ggccttccaa agtgttggga ttacaggctt gagccactgt
     6901 gcccggccca taaagcacta tttaatgact gctccataaa tacaaacttc ctttccttat
     6961 tattattatt tatttttttt ttgagatgga gtcttgctct cgtcccccag gctggagtgc
     7021 agtggcgcga tctcggctca ctgcaacctc cgcctcccgg gttcaagcga ttctcctgac
     7081 ttgggactac aggtgcgtgc gaccacagcc aactcatttt tctgttttta gcagagacag
     7141 ggtttcacca tattggccag gctggtcgaa ctcctgacct tgtgatccac ccgcctcggc
     7201 ctcccaaagt gctgggatta caggcgtgag ccacggcacc cggcccataa ttacaaactt
     7261 tctgagggac atcttgaagc acacaaaact atagacaata ttatcataac cacccctgca
     7321 aatgcctcac tcgtgttcag ccatcattta catttgaacc actcctgttt cacctacagc
     7381 ctcctcatgg cccctcccct ggagcactta aaaatggtaa tgacatgctc tctagtaatc
     7441 gttgtgtgtt actgtcaaaa ggtataactc atgcattgat gggggtaacc cctacagcag
     7501 ttaaccctgc gtgctcagag gggaatgaaa ggtagctcag aggctcggga ggtgaggttg
     7561 caaaatggct tgtgttgtgg ggaatgttct catccaccca aatcaagcat cactacatcc
     7621 atccgcaggg gaggcaagct tagggcatca cgtccaggag tggccaggtc ccgatgtcac
     7681 ggggagccgg gctgggacaa gctccagacg gccagggtgg tgtgggtttg cagtgggagg
     7741 gctggcgcgg gggccctgag ggctccctgg ttcccatctt atcaagatgc tggactcggt
     7801 gcttgaaaca tgtcctgaca gccacatgaa aagggttaga aatgatactg tttcgcctgg
     7861 gtgctgtggc tcatgcttgt aatcctagca ctttgggagg ccgaggcggg cggatcacga
     7921 ggtcaggagt tcgagaccag cctggccaac atggcgaaac cccgtctcta ctaaaaatac
     7981 aaaaattagc ctagtgtggt ggcgggcacc tgtaatccca gctacttagg aggctgaggc
     8041 aggagaatcg cttgaaccca ggaggcggag gttgtagtga gccgagattg cactactgca
     8101 ctccagcctg ggtgacagag tgagaccctg tctccaaaaa aaaaaaaaaa atgatactgt
     8161 ttcctgtatt tttttctctt agtatcttaa gaaatacatg atttgaacaa ggaccttgtg
     8221 tctcctctcc cctttcccgg actattctga ccccagcagt tctcagaagg agctgacatt
     8281 aatgagaact tctccctgtt agacccttaa aatactgagc tttcgggacc ctgcctggct
     8341 tgttcctgta aaacggtctt gtctgtggta aatactaagg tttttggtct cacagccgag
     8401 gaaatcaagg atgtggatgc acacagagtg agatttggag caggagttta ataggcaaaa
     8461 ggaagaaaca gctctgtcac agagacgggt cccgaacggg ttgtgaagtt gtagaagaaa
     8521 tgtcaggggt tttatagatg ggctagtgag gaggggtgtc ttttcttcct agggcctgaa
     8581 gaaccagtta ggaccaggtg tgctgtctgc atcgagcaga gtctctagca gcccccaccc
     8641 cattcttttt tttttttttt tttttgagat ggagtctcgc tctgtcgccc aggctggagt
     8701 gcagtggtgt catcttggct cactgcaacc tccgcctccc aggttcaagc aattctcgtg
     8761 cctcagcctc ccgcgtagtc gggattacag gtgcatgcag ccatgcccgg ctaattttgt
     8821 atttttagta gagacaaggt ttcaccatgt tggtcaggct ggtctccaac tcctgacctc
     8881 aagtgatccg cctgcctcgg cctcccaaag tagtgggatt gcaggcgtga gccaccgcgc
     8941 cgggccccca ccccattctt tgatcctgca ggcaggccct tcgtttgtgc tgctctgcgc
     9001 tgctgcttat ctgtgtgtgc taaaagggga gggagtgttt ctgtgcccat tcccaggcac
     9061 cttcgtgcag ctgcaggcat ctcccccacg cgcttccagc ttcccttagt gtgcctaaag
     9121 aaagggaaag gaatgtgctt taggcccact gttttcactg gggcccatct atgtatgtgg
     9181 cttggtgatt acccaggaac ctcccaagtc tgtgcccgag ttgcttatct gtgttttaca
     9241 gcctgaaaga tcaggctgct ctttgttagg agaagtgatt tctttgaact gcgtggtgtg
     9301 gtgagaaagg gagcaatttc taagctgctt tttgttagga agttttttgc tggggactct
     9361 cttcgcccta tctacctaaa tgatttctgt ttcttacatc aattgggtat tgatttcagg
     9421 tagaatgggt gaatgcattt attcgttcat ctaagccgca tttactcctt tcttttcttt
     9481 cactgttgtt ttttttttga gacggagtct tgcactgtca cccaggctgg agtgcagtgg
     9541 tgcgatctcg gctcactgca agctccacct cccgggttca tgccattctc ctgcctcagc
     9601 ctcccgagta gctgggacta caggcgccca ccaccacgcc cggctaagct aattttttgt
     9661 attgttagta gagacggggt ttcaccgtgt tagccaggat ggtctcaatc tgaccttgtg
     9721 attccgccca cctcggcctc ccaaagtgct gggattacag ccgtgagcca ccgcacctgg
     9781 cctctttcag ttttgatagc agattatttc ctttggccct gcgtggtctc tctctggctg
     9841 ggcagctctg ggccagccat aaatggaggg gagggcgtgt gtgtggccac gtgtgtttct
     9901 gtcctggaca acactgtccg gaggggccta agtgggaagt gccctcaacc aaggagatag
     9961 ctgctgtgtg tgtgtgtgtt gtgtgtggtt gggacagtcg gggattctca ctttttttta
    10021 aaatttgaga tggagtttca ctgtcgtcac ccaggctaga gtgcaatggt gtgatttcgg
    10081 ctcactgcaa cctctgcctc ccgggtttaa gcgattctcc tgcttcagcc tctcaagtag
    10141 ctgggattac aggtgcccgc caccacgccc agctaatttt tgtattttta gtagagacgg
    10201 ggtttcacca cgttggccag gctggtctgg aactcctgac ctcaggtgat ccacctgcct
    10261 cggcccccca aagtactggg attacaggcg tgagccactg ggcgcagcct gggatcctca
    10321 ctttttcgca gattggtcac tagcagggcg tctgatggaa ctttctgaga tgccggaaat
    10381 gttctctgtg ctgtacaaaa gggatgccac tagccacatg ccacgtagaa cagttgaaat
    10441 gtggccagca tgagattttt ttaatcttgc caatttttga tggatttaag tttaagcacc
    10501 tgcgtgtgag cggctaccgc gtgggctggg attggaggaa gctggaaagc gctcccctgc
    10561 gtttcggctg ctggcccagc agagggcgcc ggagagcgga cggagccgca ggcggggttg
    10621 tgggaagacg ctgggatccc gtgcgcgctt gtgctccttc cttctgggcc agcgcgcaga
    10681 ctatcaaccg aggtattcag tccctcggaa acccagttcc tccgtttcca ggcttctcgc
    10741 ccacacggag acagtcgctc tcacacgtac ctctcgcggg ctcttactcg tggtcccggt
    10801 ctcctcctgg tccccacctt ccagcccagc gcgcacacta gacggcacgt gctctccacg
    10861 ctctttctcc agcgcccctt gctcacccgc cccccgttgg agtggcccat cctctccgct
    10921 tagacacctg cagtcactgt cactagctga actgcgccct cctgaaagct ctatgttgaa
    10981 accctacccc ccaccctgca ccgaccccgt accccagaat ggggctgtat ttggagacag
    11041 ggcctttaca aggtaagtaa ggttaaaatg aggacattag ggtgggtcct aatccaatct
    11101 gactgttata tagtggggtt gcaccttcat gtgaggacgc cgggagaaga cagccgtctg
    11161 ggagccaagg agagaggcct tggcggaagc caaccctgca gcaccttcat cttgaatttc
    11221 tggtttccag aactgtgaga aaatccattg acgttctata gcgctcccag ctggcggtac
    11281 tttgttatga cagccctaac atgaatgcag ccatccacac tgtctggggg aaggcacaca
    11341 caccccacag cacccttgct gtctcacccc gtatcgcaaa agctcacgca tgtgtctctc
    11401 ccgagtccct ggagggtcct gccacctaca tctctctcct tggcctgccg cctgtgatgc
    11461 tcctgggccc cctgctccag caccgctccc ttccttctgt aatacagaca acagagacag
    11521 gagatggcct ggccccactt catttctgtt ccctgggaaa taagcatcgc aagtgatccg
    11581 tagaatggta gacatcatcc acttcccaag catctctggc tgaatgctgt tctcatccac
    11641 ttcccaagca tctctggctg aatgctgttc tacacatgca cgtgggaggc accagggagc
    11701 ccagaagaaa tgaaaagcct gggcagactc gcagttctgc cacacagtga atgtgctact
    11761 caagccactc agcctgggct gcagaggttg gaagcctcac tggcctgagg tttctgagta
    11821 agctctgacc caatcattga ctattaaact tcagaggtcc atggagacct cttaggagtc
    11881 tttgaaggaa cttagcatag ataccgatag ctgctgactg cagggagatg gggagatatg
    11941 ttttgtaaac ctgtccaggc aacttttttg cctacggaaa cacacaaaaa cgaaaacaaa
    12001 ccaatgaaaa aaacctctca gaacgataca gaatccagag ccactacaac acatttcatg
    12061 aagtggcgtg agcctgtaat tccagcagtt tgggaggccg gggcgaatgg atgacttgag
    12121 gtcaggagtt cgagaccagc ctggccaaca tgacaaaatc accatctcta ctaaaaatac
    12181 aaaaattagt catgcatagt ggcatgcacc tgtagtccca gctactcggg atgctgagat
    12241 gggagaatcg tttgagcccg ggaggtggag gttgcagtga gccgagatcg caccattgca
    12301 ctccagcatg gacaacagag caagaccctt tcttagaaaa aagaaaagta tccccgaaca
    12361 ttatcagagt caagttaata aaggccaaag gtagagaaaa tcttgaaagt atcgagaaaa
    12421 tgacatgtca atcataaggt caggttcttc aacagtcata ctgacattgt ggctggaata
    12481 tttgttgttg tgggatatat atatatatat atatttagag tgttgtggga tatatatata
    12541 tatttagaga caaggtcttg ctctatcact aaagctgcat tgcagtgtgt gatcatagct
    12601 caccatggga ttcttgattt tcagtgagta taaagtgtaa gacaaaataa gtcttagttc
    12661 aggtatgctg gatgggaagc cagaggtctg catagcagtt acaaagaagg ccttattatc
    12721 tggaagaaaa gggaccatag aatgtccatt ggttggggaa caacccagct ttagtttttc
    12781 cagattctct gcatgtgcag gtttatgaga ctggagaact ttgatttctt cttcctcctc
    12841 ctcctctctt ttcttcttcc tcttcctcct ttttctcctc ctttcttcct ttatttttct
    12901 ttcttccttt cctcctcttt cttctccttt ttcctcctct tttcttcctt tcttctcttt
    12961 ttcttcttct tttttttttt tttttaaaag accgtgtttc gctctgtcac ccaggctgga
    13021 atgcagtggc gtactgtttt agaaaagtga aagagggccg ggcgtggtgg ctcatgtctg
    13081 aaatcctagc actttgggag gctgaggcgg gtggatcacc tgaggttggg agttcgagac
    13141 cagcctggcc gacatggcaa aaccctgtct ctactaaaaa tacaaaaatt agccgggcat
    13201 gatggcagat gcctgtaatc tcagctcctc agtaggctaa ggcgggagaa tcgcttgaac
    13261 ccagaggcag aggctgcagt gagccaagat tgcagcattg caggaaagag agaaaaagga
    13321 gggaagggga aggaaaagga aagaaagaaa agaggggaag ggaagggaga gatttaaaaa
    13381 acaatagtaa agagagattt aaaaaacaat agtaaagaga gattaaaaaa aaaaagtaaa
    13441 tgggccaggt gcgatggctt gtgcctgtaa tcccagcagt ttgagaggct gaagtgggag
    13501 gatcacttga agccaggagt accagaccag cctgggaaac atagtgagac cccgtctata
    13561 ctaaaaaata tttaaatggg ctgagcgcgg tggctcaggc ctgtaatccc agcactttgg
    13621 gaggccgagg caggcggatt acctcaggtc aggagtttga gaccagcctg accaacatgg
    13681 caaaacccca tctctactaa aaataccaaa acaaaaacta gcagggcatg gtggcgcatg
    13741 cctgaaatcc cagctactca ggtggctgag acaggagaat cacttgaacc caggagacgg
    13801 aggctgcagt gagccgagat tgtgccactg cactccagcc tgggcgagca acagagtgag
    13861 gctgtctctc aaaaaaaaaa aaaaaggaac ggtagtgtgc acctgtggtc ccagctacac
    13921 aggaggctga ggcaggagca tcacttgaac ccaggagttc aaggctgcag tgagctatga
    13981 tggcaccact gccctccaga ctgagccaaa aagcagaccc tgactctaaa acaaaagaaa
    14041 gaacaaataa caaatgaaaa taaataccca aaaaccagtc aaatgtgaaa ctggtgtcag
    14101 gggtatgttc tccatgttga gccccttctg tggaatatga cagtgtctct atttcgggta
    14161 aaggaaagac caaaataact ttccaagatg aaaagctaag agtgctgatc tcatcacaga
    14221 catagccagc tcttcagttc agaccacctg gaaaccctcc ccatgtcttt tcagaacaat
    14281 gtcctaagaa ccatagggga agtggccata aaaaccatct ttcctttgca gactggggtg
    14341 gggactctgg ccaatgccat tctgttcttc cataatatct ttcccatctt gactgagcat
    14401 caaatgaggc ccacacacaa gattctcacc cacatggccg tggcaaactc cttggttctt
    14461 ctttccactg gaatccccca cacaatggtg tcttttgtcc tatggaaacc cttgtccagt
    14521 cttgagtgta aactcgtgtt tacaggtctt aaagtcctga cctggtgatc cgcccgcgtg
    14581 gacctcccaa agtgctggaa ttacaggcgt gagccaccac tcctggccta tgccagcatt
    14641 ctttaaagtt gtgggaaaag gccaaaagca aatagaaaat tgggcaaaaa agttcataat
    14701 tgcttaaaca tatggaaaac ctggaagtaa aattaagcaa gtaagtgcga gcactctaca
    14761 ttgaagatta tagaaaattc agcctcccaa atatccaaag aatccaggag tgtgccaaga
    14821 gatccaagag taggtccaca atttctcatt ctcaggagaa tctgagatta tatttcaatc
    14881 taatgataac tgattatata atccaatttc tgcatcatct tgcttttatg ttttgtaata
    14941 aaaattttag accaggccaa taaggcatca gcaatttacc gtagggaact agggaatgtt
    15001 taattatgac aatgaagtgt ttaatattgt gttttgaaat ataaacagtg tagatatatt
    15061 tgaataatac tgaattttta gaaaaatcta ttggggtgtc atatataaaa ggataagtag
    15121 agatttggaa atgctttttt attggacaag aaaattgatc aaatcgaaat ctggacaaac
    15181 tagaaagtct ggatgctagc aatacaatct atagacacat tctagcatca cttctgtgtc
    15241 agaagtatcc ggattgtggt gcctacaaat gcatgctcct acacaccaag acatccatta
    15301 agaaaataaa gccatgcttt ccagagcgtt tagcacttgt ttaacgtact tgtgcttcta
    15361 aatatcacaa ggcaggatgg gatttctaaa gacaagggga tataggaccg aggcaaagac
    15421 aatcagcatg tacactgaat cacactccat aatcatattg ctagaacact tagggagtac
    15481 ctctgaaatc acagccctgc tgccatgatg tgcaattaca ggatagaggt gccaggtgaa
    15541 ggtcccactg tggccaagga aagcctttgt gagacgacta cttcagggtg ttccccatca
    15601 gaggagcaca cccctgatct tgtattcctc gtcaatccaa ggtccatgtg aaatggtcag
    15661 atggggaatt ttttcttcca cagacatcag gatcttctga gtttcttcat caaagccaga
    15721 tttgtgcagc ctgggaaaat agaaataaag tttttttttt tgtttttcat ttttacacag
    15781 tattgcctct ctacattctg atgtctacct ctcctctcct ctttattctt tcctagaaat
    15841 cactgggaga attgtactga atttgaaaaa caagcatgaa tctttcctat aggataggaa
    15901 gcctctgatt tataccataa agtgaaattt catatacagg atgtatcaat taagatttaa
    15961 ttcaaaaaag atgaatttaa gttaaaaaga aaaacacagg ataacttcca ttcatctgat
    16021 gttcatatgc cctggcacat ctattgcttc ttataatgtg cttgcttaaa aaaaaaaaac
    16081 atgagctttt ctcagagaca ctaatataat gaattcatgt acccatcacc aacttctaca
    16141 attaccaata ttcagcctct tccatttaca acctgcccat tttctaagaa ataattaaag
    16201 cacatgctac acccataaa
//
