LOCUS       NM_001979               2776 bp    mRNA    linear   PRI 04-DEC-2024
DEFINITION  Homo sapiens epoxide hydrolase 2 (EPHX2), transcript variant 1,
            mRNA.
ACCESSION   NM_001979
VERSION     NM_001979.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Turnbull,J. and Chapman,V.
  TITLE     Targeting the soluble epoxide hydrolase pathway as a novel
            therapeutic approach for the treatment of pain
  JOURNAL   Curr Opin Pharmacol 78, 102477 (2024)
   PUBMED   39197248
  REMARK    GeneRIF: Targeting the soluble epoxide hydrolase pathway as a novel
            therapeutic approach for the treatment of pain.
            Review article
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Pu,Y., Cheng,R., Zhang,Q., Huang,T., Lu,C., Tang,Z., Zhong,Y.,
            Wu,L., Hammock,B.D., Hashimoto,K., Luo,Y. and Liu,Y.
  TITLE     Role of soluble epoxide hydrolase in the abnormal activation of
            fibroblast-like synoviocytes from patients with rheumatoid
            arthritis
  JOURNAL   Clin Immunol 257, 109850 (2023)
   PUBMED   38013165
  REMARK    GeneRIF: Role of soluble epoxide hydrolase in the abnormal
            activation of fibroblast-like synoviocytes from patients with
            rheumatoid arthritis.
REFERENCE   3  (bases 1 to 2776)
  AUTHORS   Gao,L., Chen,W., Li,L., Li,J., Kongling,W., Zhang,Y., Yang,X.,
            Zhao,Y., Bai,J. and Wang,F.
  TITLE     Targeting soluble epoxide hydrolase promotes osteogenic-angiogenic
            coupling via activating SLIT3/HIF-1alpha signalling pathway
  JOURNAL   Cell Prolif 56 (7), e13403 (2023)
   PUBMED   36636821
  REMARK    GeneRIF: Targeting soluble epoxide hydrolase promotes
            osteogenic-angiogenic coupling via activating SLIT3/HIF-1alpha
            signalling pathway.
REFERENCE   4  (bases 1 to 2776)
  AUTHORS   Sato,K., Emi,M., Ezura,Y., Fujita,Y., Takada,D., Ishigami,T.,
            Umemura,S., Xin,Y., Wu,L.L., Larrinaga-Shum,S., Stephenson,S.H.,
            Hunt,S.C. and Hopkins,P.N.
  TITLE     Soluble epoxide hydrolase variant (Glu287Arg) modifies plasma total
            cholesterol and triglyceride phenotype in familial
            hypercholesterolemia: intrafamilial association study in an
            eight-generation hyperlipidemic kindred
  JOURNAL   J Hum Genet 49 (1), 29-34 (2004)
   PUBMED   14673705
REFERENCE   5  (bases 1 to 2776)
  AUTHORS   Sandberg,M. and Meijer,J.
  TITLE     Structural characterization of the human soluble epoxide hydrolase
            gene (EPHX2)
  JOURNAL   Biochem Biophys Res Commun 221 (2), 333-339 (1996)
   PUBMED   8619856
REFERENCE   6  (bases 1 to 2776)
  AUTHORS   Larsson,C., White,I., Johansson,C., Stark,A. and Meijer,J.
  TITLE     Localization of the human soluble epoxide hydrolase gene (EPHX2) to
            chromosomal region 8p21-p12
  JOURNAL   Hum Genet 95 (3), 356-358 (1995)
   PUBMED   7868134
REFERENCE   7  (bases 1 to 2776)
  AUTHORS   Beetham,J.K., Tian,T. and Hammock,B.D.
  TITLE     cDNA cloning and expression of a soluble epoxide hydrolase from
            human liver
  JOURNAL   Arch Biochem Biophys 305 (1), 197-201 (1993)
   PUBMED   8342951
REFERENCE   8  (bases 1 to 2776)
  AUTHORS   Papadopoulos,D., Grondal,S., Rydstrom,J. and DePierre,J.W.
  TITLE     Levels of cytochrome P-450, steroidogenesis and microsomal and
            cytosolic epoxide hydrolases in normal human adrenal tissue and
            corresponding tumors
  JOURNAL   Cancer Biochem Biophys 12 (4), 283-291 (1992)
   PUBMED   1423213
REFERENCE   9  (bases 1 to 2776)
  AUTHORS   Petruzzelli,S., Franchi,M., Gronchi,L., Janni,A., Oesch,F.,
            Pacifici,G.M. and Giuntini,C.
  TITLE     Cigarette smoke inhibits cytosolic but not microsomal epoxide
            hydrolase of human lung
  JOURNAL   Hum Exp Toxicol 11 (2), 99-103 (1992)
   PUBMED   1349227
REFERENCE   10 (bases 1 to 2776)
  AUTHORS   Vesell,E.S.
  TITLE     Genetic factors that regulate cytosolic epoxide hydrolase activity
            in normal human lymphocytes
  JOURNAL   Ann Genet 34 (3-4), 167-172 (1991)
   PUBMED   1809223
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC331720.1, BC011628.2,
            BQ272362.1 and AF311103.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_001979.5.
            
            Summary: This gene encodes a member of the epoxide hydrolase
            family. The protein, found in both the cytosol and peroxisomes,
            binds to specific epoxides and converts them to the corresponding
            dihydrodiols. Mutations in this gene have been associated with
            familial hypercholesterolemia. Alternatively spliced transcript
            variants have been described. [provided by RefSeq, Feb 2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.14265.1,
                                           SRR7410570.425362.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000521400.6/ ENSP00000430269.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 DC331720.1         142-142
            2-2131              BC011628.2         1-2130
            2132-2134           BQ272362.1         16-18               c
            2135-2776           AF311103.5         154806-155447
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p21.2-p21.1"
     gene            1..2776
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /note="epoxide hydrolase 2"
                     /db_xref="GeneID:2053"
                     /db_xref="HGNC:HGNC:3402"
                     /db_xref="MIM:132811"
     exon            1..167
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     CDS             67..1734
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /EC_number="3.3.2.10"
                     /EC_number="3.1.3.76"
                     /note="isoform 1 is encoded by transcript variant 1;
                     epoxide hydrolase 2, cytosolic; epoxide hydrolase,
                     soluble; epoxide hydratase; epoxide hydrolase 2,
                     cytoplasmic; bifunctional epoxide hydrolase 2"
                     /codon_start=1
                     /product="bifunctional epoxide hydrolase 2 isoform 1"
                     /protein_id="NP_001970.2"
                     /db_xref="CCDS:CCDS6060.1"
                     /db_xref="GeneID:2053"
                     /db_xref="HGNC:HGNC:3402"
                     /db_xref="MIM:132811"
                     /translation="MTLRAAVFDLDGVLALPAVFGVLGRTEEALALPRGLLNDAFQKG
                     GPEGATTRLMKGEITLSQWIPLMEENCRKCSETAKVCLPKNFSIKEIFDKAISARKIN
                     RPMLQAALMLRKKGFTTAILTNTWLDDRAERDGLAQLMCELKMHFDFLIESCQVGMVK
                     PEPQIYKFLLDTLKASPSEVVFLDDIGANLKPARDLGMVTILVQDTDTALKELEKVTG
                     IQLLNTPAPLPTSCNPSDMSHGYVTVKPRVRLHFVELGSGPAVCLCHGFPESWYSWRY
                     QIPALAQAGYRVLAMDMKGYGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGH
                     DWGGMLVWYMALFYPERVRAVASLNTPFIPANPNMSPLESIKANPVFDYQLYFQEPGV
                     AEAELEQNLSRTFKSLFRASDESVLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFY
                     VQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKILIPALMVTAEKDFVLVPQMSQHME
                     DWIPHLKRGHIEDCGHWTQMDKPTEVNQILIKWLDSDARNPPVVSKM"
     exon            168..252
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            253..412
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            413..603
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            604..726
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            727..801
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            802..897
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            898..976
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1011
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1038
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            1039..1124
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1236
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            1237..1308
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1342
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            1343..1445
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1515
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1596
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            1597..1655
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..2776
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2111..2116
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /note="hexamer: AATAAA"
     polyA_site      2131
                     /gene="EPHX2"
                     /gene_synonym="ABHD20; CEH; SEH"
                     /note="major polyA site"
ORIGIN      
        1 gccctggcct tcgcgcatct cccaggttag ctgcgtgtcc gggtgctagg ctgcagaccc
       61 gccgccatga cgctgcgcgc ggccgtcttc gaccttgacg gggtgctggc gctgccagcg
      121 gtgttcggcg tcctcggccg cacggaggag gccctggcgc tgcccagagg acttctgaat
      181 gatgctttcc agaaaggggg accagagggt gccactaccc ggcttatgaa aggagagatc
      241 acactttccc agtggatacc actcatggaa gaaaactgca ggaagtgctc cgagaccgct
      301 aaagtctgcc tccccaagaa tttctccata aaagaaatct ttgacaaggc gatttcagcc
      361 agaaagatca accgccccat gctccaggca gctctcatgc tcaggaagaa aggattcact
      421 actgccatcc tcaccaacac ctggctggac gaccgtgctg agagagatgg cctggcccag
      481 ctgatgtgtg agctgaagat gcactttgac ttcctgatag agtcgtgtca ggtgggaatg
      541 gtcaaacctg aacctcagat ctacaagttt ctgctggaca ccctgaaggc cagccccagt
      601 gaggtcgttt ttttggatga catcggggct aatctgaagc cagcccgtga cttgggaatg
      661 gtcaccatcc tggtccagga cactgacacg gccctgaaag aactggagaa agtgaccgga
      721 atccagcttc tcaatacccc ggcccctctg ccgacctctt gcaatccaag tgacatgagc
      781 catgggtacg tgacagtaaa gcccagggtc cgtctgcatt ttgtggagct gggctccggc
      841 cctgctgtgt gcctctgcca tggatttccc gagagttggt attcttggag gtaccagatc
      901 cctgctctgg cccaggcagg ttaccgggtc ctagctatgg acatgaaagg ctatggagag
      961 tcatctgctc ctcccgaaat agaagaatat tgcatggaag tgttatgtaa ggagatggta
     1021 accttcctgg ataaactggg cctctctcaa gcagtgttca ttggccatga ctggggtggc
     1081 atgctggtgt ggtacatggc tctcttctac cccgagagag tgagggcggt ggccagtttg
     1141 aatactccct tcataccagc aaatcccaac atgtcccctt tggagagtat caaagccaac
     1201 ccagtatttg attaccagct ctacttccaa gaaccaggag tggctgaggc tgaactggaa
     1261 cagaacctga gtcggacttt caaaagcctc ttcagagcaa gcgatgagag tgttttatcc
     1321 atgcataaag tctgtgaagc gggaggactt tttgtaaata gcccagaaga gcccagcctc
     1381 agcaggatgg tcactgagga ggaaatccag ttctatgtgc agcagttcaa gaagtctggt
     1441 ttcagaggtc ctctaaactg gtaccgaaac atggaaagga actggaagtg ggcttgcaaa
     1501 agcttgggac ggaagatcct gattccggcc ctgatggtca cggcggagaa ggacttcgtg
     1561 ctcgttcctc agatgtccca gcacatggag gactggattc cccacctgaa aaggggacac
     1621 attgaggact gtgggcactg gacacagatg gacaagccaa ccgaggtgaa tcagatcctc
     1681 attaagtggc tggattctga tgcccggaac ccaccggtgg tctcaaagat gtagaacgca
     1741 gcgtgtgccc acgctcagca ggtgtgccat ccttccacct gctggggcac cattcttagt
     1801 atacagaggt ggccttacac acatcttgca tggatggcag cattgttctg aaggggtttg
     1861 cagaaaaaaa agattttctt tacataaagt gaatcaaatt tgacattatt ttagatccca
     1921 gagaaatcag gtgtgattag ttctccaggc atgaatgcat cgtcccttta tctgtaagaa
     1981 cccttagtgt cctgtagggg gacagaatgg ggtggccagg tggtgatttc tctttgacca
     2041 atgcatagtt tggcagaaaa atcagccgtt catttagaag aatcttagca gagattggga
     2101 tgccttactc aataaagcta agatgactat gctgctggct gtctttgttc ttggagaggt
     2161 ggagtgactg ttcacggaga atgcacggca tggggatgaa ccctttccct ctgcttttga
     2221 gccctgttct gggctccaag gtgctgtatg atctaggccg tgctggccag gagacaatcc
     2281 tatggggatg gcagtggtgt cttgtgctct gtcccctaga gcagtcactg gccacaggta
     2341 gccacctgcc tttctcccgg cttccctagc agagtttgct caggcacaaa tgtgcacctg
     2401 gagcatttga aatgtgctag tgaggctgag gaagagaatt ttaaatttca ttgaattgta
     2461 atcagtttaa agttaaatag cagcagctgc catgaggctc aggcagatcc agctcatctt
     2521 cccctctgcc ccctcctccc tatcttcccc tctaccctct cctgcctcct gtcacatccg
     2581 gttaggctcc catctcactg atgtgtctcc tgcagtggtc tctcccctgc cctgcttcca
     2641 gccccagagt cctggagcag cagcctcctc acgggtcttg ccgcccacat ggttggatcc
     2701 tgaaactcgc agggcctccc ccaactgccc ctgcccaccc ccagcccggc cccatcaccc
     2761 tccaattcct aaccgc
//
