LOCUS       NM_001309193           10942 bp    mRNA    linear   PRI 28-AUG-2024
DEFINITION  Homo sapiens EPH receptor B2 (EPHB2), transcript variant 4, mRNA.
ACCESSION   NM_001309193
VERSION     NM_001309193.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10942)
  AUTHORS   David,E.T., Yousuf,M.S., Mei,H.R., Jain,A., Krishnagiri,S.,
            Elahi,H., Venkatesan,R., Srikanth,K.D., Dussor,G., Dalva,M.B. and
            Price,T.J.
  TITLE     ephrin-B2 promotes nociceptive plasticity and hyperalgesic priming
            through EphB2-MNK-eIF4E signaling in both mice and humans
  JOURNAL   Pharmacol Res 206, 107284 (2024)
   PUBMED   38925462
  REMARK    GeneRIF: ephrin-B2 promotes nociceptive plasticity and hyperalgesic
            priming through EphB2-MNK-eIF4E signaling in both mice and humans.
REFERENCE   2  (bases 1 to 10942)
  AUTHORS   Overman,R.C., Debreczeni,J.E., Truman,C.M., McAlister,M.S. and
            Attwood,T.K.
  TITLE     Completing the structural family portrait of the human EphB
            tyrosine kinase domains
  JOURNAL   Protein Sci 23 (5), 627-638 (2014)
   PUBMED   24677421
  REMARK    GeneRIF: Comparative crystalisation analysis of the human EphB
            family kinases has yielded new crystal forms of the human EphB2 and
            EphB4 catalytic domains.
REFERENCE   3  (bases 1 to 10942)
  AUTHORS   Badzioch,M., Eeles,R., Leblanc,G., Foulkes,W.D., Giles,G.,
            Edwards,S., Goldgar,D., Hopper,J.L., Bishop,D.T., Moller,P.,
            Heimdal,K., Easton,D. and Simard,J.
  TITLE     Suggestive evidence for a site specific prostate cancer gene on
            chromosome 1p36. The CRC/BPG UK Familial Prostate Cancer Study
            Coordinators and Collaborators. The EU Biomed Collaborators
  JOURNAL   J Med Genet 37 (12), 947-949 (2000)
   PUBMED   11186936
REFERENCE   4  (bases 1 to 10942)
  AUTHORS   Gibbs,M., Stanford,J.L., McIndoe,R.A., Jarvik,G.P., Kolb,S.,
            Goode,E.L., Chakrabarti,L., Schuster,E.F., Buckley,V.A.,
            Miller,E.L., Brandzel,S., Li,S., Hood,L. and Ostrander,E.A.
  TITLE     Evidence for a rare prostate cancer-susceptibility locus at
            chromosome 1p36
  JOURNAL   Am J Hum Genet 64 (3), 776-787 (1999)
   PUBMED   10053012
REFERENCE   5  (bases 1 to 10942)
  AUTHORS   Tang,X.X., Pleasure,D.E., Brodeur,G.M. and Ikegaki,N.
  TITLE     A variant transcript encoding an isoform of the human protein
            tyrosine kinase EPHB2 is generated by alternative splicing and
            alternative use of polyadenylation signals
  JOURNAL   Oncogene 17 (4), 521-526 (1998)
   PUBMED   9696046
REFERENCE   6  (bases 1 to 10942)
  AUTHORS   Saito,T., Seki,N., Matsuda,Y., Kitahara,M., Murata,M., Kanda,N.,
            Nomura,N., Yamamoto,T. and Hori,T.A.
  TITLE     Identification of the human ERK gene as a putative receptor
            tyrosine kinase and its chromosomal localization to 1p36.1: a
            comparative mapping of human, mouse, and rat chromosomes
  JOURNAL   Genomics 26 (2), 382-384 (1995)
   PUBMED   7601466
REFERENCE   7  (bases 1 to 10942)
  AUTHORS   Fox,G.M., Holst,P.L., Chute,H.T., Lindberg,R.A., Janssen,A.M.,
            Basu,R. and Welcher,A.A.
  TITLE     cDNA cloning and tissue distribution of five human EPH-like
            receptor protein-tyrosine kinases
  JOURNAL   Oncogene 10 (5), 897-905 (1995)
   PUBMED   7898931
REFERENCE   8  (bases 1 to 10942)
  AUTHORS   Kiyokawa,E., Takai,S., Tanaka,M., Iwase,T., Suzuki,M., Xiang,Y.Y.,
            Naito,Y., Yamada,K., Sugimura,H. and Kino,I.
  TITLE     Overexpression of ERK, an EPH family receptor protein tyrosine
            kinase, in various human tumors
  JOURNAL   Cancer Res 54 (14), 3645-3650 (1994)
   PUBMED   8033077
REFERENCE   9  (bases 1 to 10942)
  AUTHORS   Iwase,T., Tanaka,M., Suzuki,M., Naito,Y., Sugimura,H. and Kino,I.
  TITLE     Identification of protein-tyrosine kinase genes preferentially
            expressed in embryo stomach and gastric cancer
  JOURNAL   Biochem Biophys Res Commun 194 (2), 698-705 (1993)
   PUBMED   7688222
REFERENCE   10 (bases 1 to 10942)
  AUTHORS   Chan,J. and Watt,V.M.
  TITLE     eek and erk, new members of the eph subclass of receptor
            protein-tyrosine kinases
  JOURNAL   Oncogene 6 (6), 1057-1061 (1991)
   PUBMED   1648701
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL158086.32, AL512444.20 and
            AL035704.9.
            
            On Jun 2, 2019 this sequence version replaced NM_001309193.1.
            
            Summary: This gene encodes a member of the Eph receptor family of
            receptor tyrosine kinase transmembrane glycoproteins. These
            receptors are composed of an N-terminal glycosylated ligand-binding
            domain, a transmembrane region and an intracellular kinase domain.
            They bind ligands called ephrins and are involved in diverse
            cellular processes including motility, division, and
            differentiation. A distinguishing characteristic of Eph-ephrin
            signaling is that both receptors and ligands are competent to
            transduce a signaling cascade, resulting in bidirectional
            signaling. This protein belongs to a subgroup of the Eph receptors
            called EphB. Proteins of this subgroup are distinguished from other
            members of the family by sequence homology and preferential binding
            affinity for membrane-bound ephrin-B ligands. Allelic variants are
            associated with prostate and brain cancer susceptibility.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, May 2015].
            
            Transcript Variant: This variant (4) uses an alternate in-frame
            splice site at an internal exon and alternate splice sites in the
            3' coding region and 3' UTR, which results in a longer and novel 3'
            coding region compared to variant 2. It encodes isoform 4, which is
            longer than and has a distinct C-terminus compared to isoform 2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF025304.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-205               AL158086.32        83556-83760
            206-270             AL512444.20        25897-25961
            271-955             AL512444.20        28868-29552
            956-1111            AL512444.20        107513-107668
            1112-1447           AL512444.20        109353-109688
            1448-1572           AL035704.9         2246-2370
            1573-1735           AL035704.9         12771-12933
            1736-1844           AL035704.9         15359-15467
            1845-1909           AL035704.9         16301-16365
            1910-2032           AL035704.9         25874-25996
            2033-2280           AL035704.9         26597-26844
            2281-2496           AL035704.9         27840-28055
            2497-2646           AL035704.9         28909-29058
            2647-2840           AL035704.9         30269-30462
            2841-2996           AL035704.9         32331-32486
            2997-3099           AL035704.9         33349-33451
            3100-10942          AL035704.9         33545-41387
FEATURES             Location/Qualifiers
     source          1..10942
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.12"
     gene            1..10942
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /note="EPH receptor B2"
                     /db_xref="GeneID:2048"
                     /db_xref="HGNC:HGNC:3393"
                     /db_xref="MIM:600997"
     exon            1..205
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     CDS             145..3312
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /EC_number="2.7.10.1"
                     /note="isoform 4 precursor is encoded by transcript
                     variant 4; elk-related tyrosine kinase; ephrin type-B
                     receptor 2; protein-tyrosine kinase HEK5; eph tyrosine
                     kinase 3; EPH-like kinase 5; tyrosine-protein kinase
                     TYRO5; renal carcinoma antigen NY-REN-47; tyrosine-protein
                     kinase receptor EPH-3; developmentally-regulated
                     Eph-related tyrosine kinase"
                     /codon_start=1
                     /product="ephrin type-B receptor 2 isoform 4 precursor"
                     /protein_id="NP_001296122.1"
                     /db_xref="CCDS:CCDS81279.1"
                     /db_xref="GeneID:2048"
                     /db_xref="HGNC:HGNC:3393"
                     /db_xref="MIM:600997"
                     /translation="MALRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWE
                     EVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPS
                     VPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKIN
                     TEVRSFGPVSRSGFYLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTS
                     LVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGT
                     FKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISS
                     VNETSLMLEWTPPRDSGGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPR
                     IYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVD
                     SITLSWSQPDQPNGVILDYELQYYEKELSEYNATAIKSPTNTVTVQGLKAGAIYVFQV
                     RARTVAGYGRYSGKMYFQTMTEAEYQTSIQEKLPLIIGSSAAGLVFLIAVVVIAIVCN
                     RRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIE
                     QVIGAGEFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNV
                     IHLEGVVTKSTPVMIITEFMENGSLDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLADM
                     NYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAI
                     QYRKFTSASDVWSYGIVMWEVMSYGERPYWDMTNQDVINAIEQDYRLPPPMDCPSALH
                     QLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSGINLPLLDRTIPDYTS
                     FNTVDEWLEAIKMGQYKESFANAGFTSFDVVSQMMMEDILRVGVTLAGHQKKILNSIQ
                     VMRAQMNQIQSVEGQPLARRPRATGRTKRCQPRDVTKKTCNSNDGKKKGMGKKKTDPG
                     RGREIQGIFFKEDSHKESNDCSCGG"
     exon            206..270
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     exon            271..955
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1111
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1447
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1572
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1735
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     exon            1736..1844
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..1909
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..2032
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     exon            2033..2280
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     exon            2281..2496
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     exon            2497..2646
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     exon            2647..2840
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     exon            2841..2996
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     exon            2997..3099
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     exon            3100..10942
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4007..4012
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /note="hexamer: AATGAA"
     polyA_site      4033
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /note="major polyA site"
     regulatory      4093..4098
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /note="hexamer: AATACA"
     polyA_site      4121
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /note="major polyA site"
     regulatory      4748..4753
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /note="hexamer: AATAAA"
     polyA_site      4767
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /note="major polyA site"
     regulatory      10919..10924
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
                     /note="hexamer: AATAAA"
     polyA_site      10942
                     /gene="EPHB2"
                     /gene_synonym="BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5;
                     PCBC; Tyro5"
ORIGIN      
        1 attctgctgg ctgcgcggtg gcggcggctg tgtgtgcgcc gcgccttgcc gccccccctg
       61 gccccccgag cccggggcgc gcgctcccgc ccgggccgtc cgggccccgc ggcgccgcgg
      121 cccgaggccc cgggaagcgc agccatggct ctgcggaggc tgggggccgc gctgctgctg
      181 ctgccgctgc tcgccgccgt ggaagaaacg ctaatggact ccactacagc gactgctgag
      241 ctgggctgga tggtgcatcc tccatcaggg tgggaagagg tgagtggcta cgatgagaac
      301 atgaacacga tccgcacgta ccaggtgtgc aacgtgtttg agtcaagcca gaacaactgg
      361 ctacggacca agtttatccg gcgccgtggc gcccaccgca tccacgtgga gatgaagttt
      421 tcggtgcgtg actgcagcag catccccagc gtgcctggct cctgcaagga gaccttcaac
      481 ctctattact atgaggctga ctttgactcg gccaccaaga ccttccccaa ctggatggag
      541 aatccatggg tgaaggtgga taccattgca gccgacgaga gcttctccca ggtggacctg
      601 ggtggccgcg tcatgaaaat caacaccgag gtgcggagct tcggacctgt gtcccgcagc
      661 ggcttctacc tggccttcca ggactatggc ggctgcatgt ccctcatcgc cgtgcgtgtc
      721 ttctaccgca agtgcccccg catcatccag aatggcgcca tcttccagga aaccctgtcg
      781 ggggctgaga gcacatcgct ggtggctgcc cggggcagct gcatcgccaa tgcggaagag
      841 gtggatgtac ccatcaagct ctactgtaac ggggacggcg agtggctggt gcccatcggg
      901 cgctgcatgt gcaaagcagg cttcgaggcc gttgagaatg gcaccgtctg ccgaggttgt
      961 ccatctggga ctttcaaggc caaccaaggg gatgaggcct gtacccactg tcccatcaac
     1021 agccggacca cttctgaagg ggccaccaac tgtgtctgcc gcaatggcta ctacagagca
     1081 gacctggacc ccctggacat gccctgcaca accatcccct ccgcgcccca ggctgtgatt
     1141 tccagtgtca atgagacctc cctcatgctg gagtggaccc ctccccgcga ctccggaggc
     1201 cgagaggacc tcgtctacaa catcatctgc aagagctgtg gctcgggccg gggtgcctgc
     1261 acccgctgcg gggacaatgt acagtacgca ccacgccagc taggcctgac cgagccacgc
     1321 atttacatca gtgacctgct ggcccacacc cagtacacct tcgagatcca ggctgtgaac
     1381 ggcgttactg accagagccc cttctcgcct cagttcgcct ctgtgaacat caccaccaac
     1441 caggcagctc catcggcagt gtccatcatg catcaggtga gccgcaccgt ggacagcatt
     1501 accctgtcgt ggtcccagcc ggaccagccc aatggcgtga tcctggacta tgagctgcag
     1561 tactatgaga aggagctcag tgagtacaac gccacagcca taaaaagccc caccaacacg
     1621 gtcaccgtgc agggcctcaa agccggcgcc atctatgtct tccaggtgcg ggcacgcacc
     1681 gtggcaggtt acgggcgcta cagcggcaag atgtacttcc agaccatgac agaagccgag
     1741 taccagacaa gcatccagga gaagttgcca ctcatcatcg gctcctcggc cgctggcctg
     1801 gtcttcctca ttgctgtggt tgtcatcgcc atcgtgtgta acagacgggg gtttgagcgt
     1861 gctgactcgg agtacacgga caagctgcaa cactacacca gtggccacat gaccccaggc
     1921 atgaagatct acatcgatcc tttcacctac gaggacccca acgaggcagt gcgggagttt
     1981 gccaaggaaa ttgacatctc ctgtgtcaaa attgagcagg tgatcggagc aggggagttt
     2041 ggcgaggtct gcagtggcca cctgaagctg ccaggcaaga gagagatctt tgtggccatc
     2101 aagacgctca agtcgggcta cacggagaag cagcgccggg acttcctgag cgaagcctcc
     2161 atcatgggcc agttcgacca tcccaacgtc atccacctgg agggtgtcgt gaccaagagc
     2221 acacctgtga tgatcatcac cgagttcatg gagaatggct ccctggactc ctttctccgg
     2281 caaaacgatg ggcagttcac agtcatccag ctggtgggca tgcttcgggg catcgcagct
     2341 ggcatgaagt acctggcaga catgaactat gttcaccgtg acctggctgc ccgcaacatc
     2401 ctcgtcaaca gcaacctggt ctgcaaggtg tcggactttg ggctctcacg ctttctagag
     2461 gacgatacct cagaccccac ctacaccagt gccctgggcg gaaagatccc catccgctgg
     2521 acagccccgg aagccatcca gtaccggaag ttcacctcgg ccagtgatgt gtggagctac
     2581 ggcattgtca tgtgggaggt gatgtcctat ggggagcggc cctactggga catgaccaac
     2641 caggatgtaa tcaatgccat tgagcaggac tatcggctgc caccgcccat ggactgcccg
     2701 agcgccctgc accaactcat gctggactgt tggcagaagg accgcaacca ccggcccaag
     2761 ttcggccaaa ttgtcaacac gctagacaag atgatccgca atcccaacag cctcaaagcc
     2821 atggcgcccc tctcctctgg catcaacctg ccgctgctgg accgcacgat ccccgactac
     2881 accagcttta acacggtgga cgagtggctg gaggccatca agatggggca gtacaaggag
     2941 agcttcgcca atgccggctt cacctccttt gacgtcgtgt ctcagatgat gatggaggac
     3001 attctccggg ttggggtcac tttggctggc caccagaaaa aaatcctgaa cagtatccag
     3061 gtgatgcggg cgcagatgaa ccagattcag tctgtggagg gccagccact cgccaggagg
     3121 ccacgggcca cgggaagaac caagcggtgc cagccacgag acgtcaccaa gaaaacatgc
     3181 aactcaaacg acggaaaaaa aaagggaatg ggaaaaaaga aaacagatcc tgggaggggg
     3241 cgggaaatac aaggaatatt ttttaaagag gattctcata aggaaagcaa tgactgttct
     3301 tgcgggggat aaaaaagggc ttgggagatt catgcgatgt gtccaatcgg agacaaaagc
     3361 agtttctctc caactccctc tgggaaggtg acctggccag agccaagaaa cactttcaga
     3421 aaaacaaatg tgaaggggag agacaggggc cgcccttggc tcctgtccct gctgctcctc
     3481 taggcctcac tcaacaacca agcgcctgga ggacgggaca gatggacaga cagccaccct
     3541 gagaacccct ctgggaaaat ctattcctgc caccactggg caaacagaag aatttttctg
     3601 tctttggaga gtattttaga aactccaatg aaagacactg tttctcctgt tggctcacag
     3661 ggctgaaagg ggcttttgtc ctcctgggtc agggagaacg cggggacccc agaaaggtca
     3721 gccttcctga ggatgggcaa cccccaggtc tgcagctcca ggtacatatc acgcgcacag
     3781 cctggcagcc tggccctcct ggtgcccact cccgccagcc cctgcctcga ggactgatac
     3841 tgcagtgact gccgtcagct ccgactgccg ctgagaaggg ttgatcctgc atctgggttt
     3901 gtttacagca attcctggac tcgggggtat tttggtcaca gggtggtttt ggtttagggg
     3961 gtttgtttgt tgggttgttt tttgtttttt ggtttttttt aatgacaatg aagtgacact
     4021 ttgacatttc ctaccttttg aggacttgat ccttctccag gaagaaggtg ctttctgctt
     4081 actgacttag gcaatacacc aagggcgaga ttttatatgc acatttctgg atttttttat
     4141 acggttttca ttgacactct tccctcctcc cacctgccac caggcctcac caaagcccac
     4201 tgccatgggg ccatctgggc cattcagaga ctggagtgag atttgggtgt ggagggggag
     4261 gcgccaaggt ggaggagctt cccactccag gactgttgat gaaagggaca gattgaggag
     4321 gaagtgggct ctgaggctgc agggctggaa gtccttgccc acttcccact ctcctgcccc
     4381 aatctatcta gtacttccca ggcaaatagg cccctttgag gctcctgagt gccctcagat
     4441 ggtcaaaacc cagttttccc tctgggagcc taaaccaggc tgcatcggag gccaggaccc
     4501 ggatcattca ctgtgatacc ctgccctcca gagggtgcgc tcagagacac gggcaagcat
     4561 gcctcttccc ttccctggag agaaagtgtg tgatttctct cccacctcct tccccccacc
     4621 agacctttgc tgggcctaaa ggtcttggcc atggggacgc cctcagtcta gggatctggc
     4681 cacagactcc ctcctgtgaa ccaacacaga cacccaagca gagcaatcag ttagtgaatt
     4741 gaatggaaat aaacgcttta gttataatat gacccctgtt ctctgtaagt tccaagggag
     4801 accttggtac ccttgggatg cacgtgaccc aggtgctaag ggttgctctt ctaagcagcc
     4861 cttcctccct caggcttcca gaaagcccag ggcagagaaa acacatggag aacgacagct
     4921 ccacgtggcc tttctggccc tggcttgtca aaagcacagg caacaaagga agtgagttgg
     4981 aagtggctca gaactcagcc ttcgatgccc tgggaatcag ggtgagcccc tgcaggtaca
     5041 taagtagcag agaattgacg agagtgcact cggaagaggg tctgagtgtc aggtgagctg
     5101 tcatagtggg aacgtgaggg tcttagagtg ctcatgtacc cccaggcaca gccaactctg
     5161 gttttctctg tgcaaatgag ctcttgctca cattaaaatc agaattgtcc acgtggcagg
     5221 aggaggcagg gagatggatg tgccatggat atcgggtctg gctgaggaag gggagccaag
     5281 gagactacct ctcgggacct ttcccagatc cgaggagatg gccagtgaga aagattcggg
     5341 ccacaggcag ccagctagaa gcgccacggt cagagtgaac atccagcaca gcacagccca
     5401 tcgtctgtca ctcacctgtt ccgggagcct gagctgcaaa gggaaggaag ggattagaga
     5461 gaagtgtggg ccccccatgg ggacagcagg gcaagccaca gggaaagcat tggcaggttt
     5521 cccacaaact ggccggcagt ggcccactgg ccttcacttt gtgccccaaa ggtgccctgc
     5581 gcgggtggca gctgtctcgg cacacaaggt caccttctgg tgggagctgc cctgatgctc
     5641 tttagattag ttctgtctcc ccagcccacc ccccaactgt tctggggccc cacatggggc
     5701 tggcagtcgg tgcttcccgt cagatggctt cctgtcctaa gccgctgagc tggtgaccac
     5761 tagccagcat gtctgcccct ccaccctcca gggccgaggt cctcagtggc catggccccc
     5821 tgcaaacctg cttcccagag cacaggtgct accagacctc tgtgtctcca cttcagggta
     5881 acaaacagat gtcactgtga ctgtcccagg ggtacaggcg ctgctcacat ctcttccacc
     5941 cccacccacc caccccaccc gctaccgggg cagtcgcccc gccccaacct cttgcttgct
     6001 gctcagcggg gagtggcagg ccatgttgcc aagcgccttc cacttacacc acccgctcac
     6061 ccaggcacac agctctgagc cacccgctgc ctggttacta atcctttcct ggctcaacct
     6121 ccccaagtgc cagcccccgc ctgcccagca gtgttagggg agtttccggc tgctctgcac
     6181 acagttggta ctgcaggaaa gaaagctgcc ttgtaaacac aggccagggc accgtggaaa
     6241 tggggaccag ctgctctggg agggtgcagc tagcagagct gtgcagcgcc aggctgggta
     6301 ggagagagcc atgctaccca cccgcacccc tactgctacg aagcccccag aatgggtcat
     6361 tgttggtgct cagcagtcct ctccctcggg gatgatatcc aaaatatcaa ctgcgaagac
     6421 tcgggcagac caatcagaat agatgatggc ctgagtgctg gaccagaggc ctcagagcag
     6481 ggacccgggt ggcggggcag taggagacac ttaagggact cagggctatg gctgtttact
     6541 aaccagcagt gcctcttact cagatggcat cagctgaact ttccccatca tttctagttg
     6601 gtccccaccc tgccaccatc taggggcttc tctgccacca ccttgtgagc agttgctagc
     6661 ctcacactcc tctcccatca ggggctcatc tccaaaaggc tgggagctcc cgccctgccc
     6721 agttaatcct cctgcctcac tgaagcacag gaaggaccca ggccccagac catcacccac
     6781 cccagggctg ggccctgggc cactcctggc tcactccagg gcccctgttg tttgaagatg
     6841 gtaccaaagg ctggaagact cttgctaggg aagataacgt aaatgcattc aaaagacagg
     6901 gtaccacatg atgctaggga aagtgcgtca tgaccgcagg gtagcagcct gctcctgtca
     6961 ctagggatgt gaatctgggg agacacttcc cctcactgag ccatctgtaa aatgagagca
     7021 ttggactggg tgaggcaggc tgtgcaaaac acttatacac cttagtccca tgaggaagga
     7081 gtattccccc tgcatatttt ggggggaaac tgaggctcag ggggatgaag tactttgctc
     7141 agggtaacac agccaggaag gagcagagca aggccatgag ctttggattt ttctgtttcc
     7201 aatgtctcct ctaccgtgct caattagatg atgagaaaca gttggtgtct gatgctggcc
     7261 aataagagaa aagaaagctc aagtgggctg gggctgtcag ggagggcttc atggagaggc
     7321 tgtcttgaaa atgacagagg tcaaaagaaa ggtcactcca agacgcagct gccagaatgg
     7381 tccaacacag tgaggagttg tgtctggatg ggccagtgga acgggggaag tgaaggttga
     7441 tataagtgga ggttgaggca gtttaggcgt gatgtacagt ccagccacag caggttcttg
     7501 agcaggaggg tagcatagtg agcatcaggt tctaggaaga agcaccagtt cagccatcag
     7561 atggggcagg atgcctccca gctactcctc tccccgagaa ggaattgccc ccggagcggc
     7621 cctcatttat tccaggagaa gccaggccct gctgcttcag tttccatcct cactgacgca
     7681 gataatccaa agagtcactt cagctgcttg gaacagcagg gttgggtctt gtgctacctg
     7741 attcttaatt tggctgatgt ctaccccatt tcctccaggc ttctctagag gtggcagggg
     7801 tggggtgggg agaaagctgc cagacaccaa aggagctgag cctggcgggg gcgggcgtgc
     7861 gggtgggcat gagaaattgc tcaggaaacc catccctgcc ctggtcaccc tagtcctgga
     7921 gagatcaccc agacatgttg gaatgagtct ggggtcctgt cttttccaat aatgtgttat
     7981 cctggggaaa cccagaggga agttgaggga gccccactgt gctgtgtgat gctacatacg
     8041 catgcgaata caccaccggg tggccttgac ccagccttct gcaaaatggc ttctgccatc
     8101 aaacttaagg tggagaatgg tcttgcagct gccagttttt ccttcagaga aactaaggtc
     8161 agagtgttgg ctgcagaaaa ggacttggcc tttgtcacat aacaggaaag agagtcaact
     8221 ccgggcacac cccctcggag gctaatctca ggaggttgga accacaggga ttgcaataat
     8281 cgctgaatac ttaccatatg ccaggtgcca aagcattatc ctctccactt tatgagtgag
     8341 gaaactgatt cccagaggtg attcccagaa gattccaacc aggccatctg tctgtagtcc
     8401 ttccttccca cctcctggga tttctcaaaa tgtttgccat cgcccacctg tatcagaatc
     8461 tcctgggcta tttattgttt aacttgcaga ctcctgggcg ccaccccaac cccctaaatc
     8521 agaatccctg gggttgggcc caagagtctc cattttttga caagctcctc caggagattc
     8581 ctccagacat ggaaatcctc acttgactct tcccccataa caaactgccc tgatgtctgc
     8641 agagagaggg tctgctgtag acagggatta agaaaaagca ggacattttc agataggatg
     8701 cccaaaccca gacgcagatt aggacccatg aagtaggggg tagagctgac cctcaggaat
     8761 gtgcccagac agagctaagc tttcaggaaa attagaaaga ccctcagacc cagagtctgt
     8821 ctgagtttag cttcgcagca tgaaggctgc cttgagaaag ctgatataac aagaagcagc
     8881 agaaatttaa tactttggag gggcccagca acccactcag ggccaccctg ggagacccca
     8941 cagaccccaa ggctatggtc cagacctgtt cactcaacac ctggccaggc ggggcctcca
     9001 gctggaccta gagctacaga tgccccacca ccactaccaa gcccggctac ccagaccaag
     9061 acacggcttt tcctggagga gagagagagc aaaggccctg tctctaccca agacctaacg
     9121 gcccctgttg aaggagaggg agcaggagag ggaggaggga ggaggtcaca gccaggacca
     9181 catacactct tgggggccct gctgagactg caagagtcat gaattctaac gttccacagg
     9241 tgaaagattc caagattcta ggattgcaaa accccatcat tctaagattc caatggaatg
     9301 attccatgtt tctaagattc catcacacta tgattctatg ctgctaattc ttgaaatttc
     9361 gagtctcatt gttcgttgct gttccccaga cctgtgggcc ctagcatttt tttaaaagca
     9421 caaaaaaaaa agagcaagaa agagagaaag agatggggag ggagacagct gaaaacagat
     9481 actaagaaaa gctccatccc ttggatctga gttacagatg caccttggca agggccaaag
     9541 accctccttt tgagtggggt gcagaacgtc tttccctggt agagtggcag aattgcatgc
     9601 atcaggcctt cctgggggta aaaggggctg gctgttccag gctacagctg agtaaagccc
     9661 cacacaggcc acagtgccca ctggctggtg gacctaggaa ccaagcaggg ccccactggc
     9721 tcagtctggg gagggactca tctggggtgg aatttccctc cctgcagcaa ggaagctgca
     9781 gggccaggaa tttgccttgg gagaccccca ctgaggaata tttccgaagc agaacccctt
     9841 cctattcaga gccagagtct taacactgga caaccacagg gtgttgctgc aaactccaga
     9901 gccaggtgcc ttccctctga catttgggac atagcttcca tggccacaca gccttcgccc
     9961 ccttccaaga ccccccttga cctttctaat cttagtcact gccttccaga gctgggaggc
    10021 cacacggcag aggtgcctgt gaatcactcc gtcatcagcc tggccgcttt cccctctgtg
    10081 gagagggact ctgatgggca gggggcacca agttgagcct ctgaggctgg cccagcatag
    10141 gccaggcagg gacattcact cacaaccagc ctttttggac tctagagaag agtaattacc
    10201 acttggtcat gcccagcccc caaatcataa tcatagctgc tatttcttga gtgccagcaa
    10261 tgtgccaggc accgtgccca actttttata cacaatgtct tgcctaatcc tcacacctct
    10321 gtgagacagg tacaattatt actcccattc cacagactag caaactcaga gacgtgacat
    10381 ggtggctctc ccaggtcaca cagccagtaa gtggcctctg accccagtac cccccactta
    10441 atcaccatgc tatgctgcta ccagtggtac acactcagcc cctctccact gtcagatcct
    10501 ttgccagtca aatcccagat ccccgggggg gcactgtggc accctctttg agcgtggcct
    10561 ccctggagct gctgtcagag ccatgggctg tgggagtgag tgtgcacact ccttctcctg
    10621 aagtgtctgc cccagcctga gctatgcaga ccggagctct caaccagggt atggggctgt
    10681 ctggcagggt ttcccagaac cctgtttcct gtggtcataa atatgggttt tcctgacaat
    10741 gttgggaggt cagggactga tacagctggg gttccaaccg ccgtgggtca gacctggctc
    10801 ctccggaccc cactgctgtg accaaggaaa tctcaccagc tgtgtccaag atcaaccctg
    10861 accctgttac tccaatgcct ttgtaggagt atttttagca gaaccgtttt tttcccaaaa
    10921 taaatgtgaa ttgtaaaaat ta
//
