LOCUS       NM_004441               4674 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens EPH receptor B1 (EPHB1), mRNA.
ACCESSION   NM_004441
VERSION     NM_004441.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Kundu,S., Nunes,L., Adler,J., Mathot,L., Stoimenov,I. and
            Sjoblom,T.
  TITLE     Recurring EPHB1 mutations in human cancers alter receptor
            signalling and compartmentalisation of colorectal cancer cells
  JOURNAL   Cell Commun Signal 21 (1), 354 (2023)
   PUBMED   38102712
  REMARK    GeneRIF: Recurring EPHB1 mutations in human cancers alter receptor
            signalling and compartmentalisation of colorectal cancer cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Ahmed,M.S., Wang,P., Nguyen,N.U.N., Nakada,Y., Menendez-Montes,I.,
            Ismail,M., Bachoo,R., Henkemeyer,M., Sadek,H.A. and Kandil,E.S.
  TITLE     Identification of tetracycline combinations as EphB1 tyrosine
            kinase inhibitors for treatment of neuropathic pain
  JOURNAL   Proc Natl Acad Sci U S A 118 (10) (2021)
   PUBMED   33627480
  REMARK    GeneRIF: Identification of tetracycline combinations as EphB1
            tyrosine kinase inhibitors for treatment of neuropathic pain.
REFERENCE   3  (bases 1 to 4674)
  AUTHORS   Jin,F., Zhao,L., Hu,Q. and Qi,F.
  TITLE     Peripheral EphrinB1/EphB1 signalling attenuates muscle hyperalgesia
            in MPS patients and a rat model of taut band-associated persistent
            muscle pain
  JOURNAL   Mol Pain 16, 1744806920984079 (2020)
   PUBMED   33356837
  REMARK    GeneRIF: Peripheral EphrinB1/EphB1 signalling attenuates muscle
            hyperalgesia in MPS patients and a rat model of taut
            band-associated persistent muscle pain.
REFERENCE   4  (bases 1 to 4674)
  AUTHORS   Trease,A.J., Li,H., Spagnol,G., Zheng,L., Stauch,K.L. and
            Sorgen,P.L.
  TITLE     Regulation of Connexin32 by ephrin receptors and T-cell
            protein-tyrosine phosphatase
  JOURNAL   J Biol Chem 294 (1), 341-350 (2019)
   PUBMED   30401746
  REMARK    GeneRIF: EphB1 and EphA1 phosphorylate the Cx32CT domain residue
            Tyr(243) Unlike for Cx43, the tyrosine phosphorylation of the
            Cx32CT increased gap junction intercellular communication.
REFERENCE   5  (bases 1 to 4674)
  AUTHORS   Chen,Q., Deng,R., Zhao,X., Yuan,H., Zhang,H., Dou,J., Chen,R.,
            Jin,H., Wang,Y., Huang,J. and Yu,J.
  TITLE     Sumoylation of EphB1 Suppresses Neuroblastoma Tumorigenesis via
            Inhibiting PKCgamma Activation
  JOURNAL   Cell Physiol Biochem 45 (6), 2283-2292 (2018)
   PUBMED   29550816
  REMARK    GeneRIF: SUMOylation of EphB1 repressed activation of its
            downstream signaling molecule PKC-gamma, and consequently inhibited
            neuroblastoma tumorigenesis.
REFERENCE   6  (bases 1 to 4674)
  AUTHORS   Gale,N.W., Holland,S.J., Valenzuela,D.M., Flenniken,A., Pan,L.,
            Ryan,T.E., Henkemeyer,M., Strebhardt,K., Hirai,H., Wilkinson,D.G.,
            Pawson,T., Davis,S. and Yancopoulos,G.D.
  TITLE     Eph receptors and ligands comprise two major specificity subclasses
            and are reciprocally compartmentalized during embryogenesis
  JOURNAL   Neuron 17 (1), 9-19 (1996)
   PUBMED   8755474
REFERENCE   7  (bases 1 to 4674)
  AUTHORS   Cerretti,D.P., Vanden Bos,T., Nelson,N., Kozlosky,C.J., Reddy,P.,
            Maraskovsky,E., Park,L.S., Lyman,S.D., Copeland,N.G., Gilbert,D.J.
            et al.
  TITLE     Isolation of LERK-5: a ligand of the eph-related receptor tyrosine
            kinases
  JOURNAL   Mol Immunol 32 (16), 1197-1205 (1995)
   PUBMED   8559144
REFERENCE   8  (bases 1 to 4674)
  AUTHORS   Tang,X.X., Biegel,J.A., Nycum,L.M., Yoshioka,A., Brodeur,G.M.,
            Pleasure,D.E. and Ikegaki,N.
  TITLE     cDNA cloning, molecular characterization, and chromosomal
            localization of NET(EPHT2), a human EPH-related receptor
            protein-tyrosine kinase gene preferentially expressed in brain
  JOURNAL   Genomics 29 (2), 426-437 (1995)
   PUBMED   8666391
REFERENCE   9  (bases 1 to 4674)
  AUTHORS   Davis,S., Gale,N.W., Aldrich,T.H., Maisonpierre,P.C., Lhotak,V.,
            Pawson,T., Goldfarb,M. and Yancopoulos,G.D.
  TITLE     Ligands for EPH-related receptor tyrosine kinases that require
            membrane attachment or clustering for activity
  JOURNAL   Science 266 (5186), 816-819 (1994)
   PUBMED   7973638
REFERENCE   10 (bases 1 to 4674)
  AUTHORS   Beckmann,M.P., Cerretti,D.P., Baum,P., Vanden Bos,T., James,L.,
            Farrah,T., Kozlosky,C., Hollingsworth,T., Shilling,H.,
            Maraskovsky,E. et al.
  TITLE     Molecular characterization of a family of ligands for eph-related
            tyrosine kinase receptors
  JOURNAL   EMBO J 13 (16), 3757-3762 (1994)
   PUBMED   8070404
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC016931.21, AC016951.9,
            AC073244.13 and AC092969.7.
            
            On Nov 23, 2018 this sequence version replaced NM_004441.4.
            
            Summary: Ephrin receptors and their ligands, the ephrins, mediate
            numerous developmental processes, particularly in the nervous
            system. Based on their structures and sequence relationships,
            ephrins are divided into the ephrin-A (EFNA) class, which are
            anchored to the membrane by a glycosylphosphatidylinositol linkage,
            and the ephrin-B (EFNB) class, which are transmembrane proteins.
            The Eph family of receptors are divided into 2 groups based on the
            similarity of their extracellular domain sequences and their
            affinities for binding ephrin-A and ephrin-B ligands. Ephrin
            receptors make up the largest subgroup of the receptor tyrosine
            kinase (RTK) family. The protein encoded by this gene is a receptor
            for ephrin-B family members. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.66964.1,
                                           SRR1803614.251394.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2144120
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000398015.8/ ENSP00000381097.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-430               AC016931.21        153173-153602
            431-495             AC016951.9         125764-125828       c
            496-1177            AC016951.9         99592-100273        c
            1178-1333           AC073244.13        52862-53017         c
            1334-1669           AC073244.13        26416-26751         c
            1670-1794           AC073244.13        5189-5313           c
            1795-1957           AC092969.7         4554-4716
            1958-2066           AC092969.7         8504-8612
            2067-2131           AC092969.7         9478-9542
            2132-2254           AC092969.7         22396-22518
            2255-2502           AC092969.7         35112-35359
            2503-2718           AC092969.7         44010-44225
            2719-2868           AC092969.7         83684-83833
            2869-3062           AC092969.7         90852-91045
            3063-3218           AC092969.7         91872-92027
            3219-4674           AC092969.7         101548-103003
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q22.2"
     gene            1..4674
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /note="EPH receptor B1"
                     /db_xref="GeneID:2047"
                     /db_xref="HGNC:HGNC:3392"
                     /db_xref="MIM:600600"
     exon            1..430
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /inference="alignment:Splign:2.1.0"
     CDS             373..3327
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /EC_number="2.7.10.1"
                     /note="ephrin type-B receptor 1; eph tyrosine kinase 2;
                     EK6; EPH-like kinase 6; tyrosine-protein kinase receptor
                     EPH-2; neuronally-expressed EPH-related tyrosine kinase"
                     /codon_start=1
                     /product="ephrin type-B receptor 1 precursor"
                     /protein_id="NP_004432.1"
                     /db_xref="CCDS:CCDS46921.1"
                     /db_xref="GeneID:2047"
                     /db_xref="HGNC:HGNC:3392"
                     /db_xref="MIM:600600"
                     /translation="MALDYLLLLLLASAVAAMEETLMDTRTATAELGWTANPASGWEE
                     VSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNV
                     PGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNT
                     EVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSL
                     VIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFK
                     ASQEAEGCSHCPSNSRSPAEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVN
                     ETSIILEWHPPRETGGRDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVS
                     ISSLWAHTPYTFDIQAINGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSI
                     TLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRA
                     RTVAGYGKFSGKMCFQTLTDDDYKSELREQLPLIAGSAAAGVVFVVSLVAISIVCSRK
                     RAYSKEAVYSDKLQHYSTGRGSPGMKIYIDPFTYEDPNEAVREFAKEIDVSFVKIEEV
                     IGAGEFGEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHPNIIR
                     LEGVVTKSRPVMIITEFMENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLAEMNY
                     VHRDLAARNILVNSNLVCKVSDFGLSRYLQDDTSDPTYTSSLGGKIPVRWTAPEAIAY
                     RKFTSASDVWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPMDCPAALHQL
                     MLDCWQKDRNSRPRFAEIVNTLDKMIRNPASLKTVATITAVPSQPLLDRSIPDFTAFT
                     TVDDWLSAIKMVQYRDSFLTAGFTSLQLVTQMTSEDLLRIGITLAGHQKKILNSIHSM
                     RVQISQSPTAMA"
     exon            431..495
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /inference="alignment:Splign:2.1.0"
     exon            496..1177
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1333
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1669
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /inference="alignment:Splign:2.1.0"
     exon            1670..1794
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /inference="alignment:Splign:2.1.0"
     exon            1795..1957
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /inference="alignment:Splign:2.1.0"
     exon            1958..2066
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..2131
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /inference="alignment:Splign:2.1.0"
     exon            2132..2254
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..2502
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /inference="alignment:Splign:2.1.0"
     exon            2503..2718
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /inference="alignment:Splign:2.1.0"
     exon            2719..2868
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /inference="alignment:Splign:2.1.0"
     exon            2869..3062
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /inference="alignment:Splign:2.1.0"
     exon            3063..3218
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /inference="alignment:Splign:2.1.0"
     exon            3219..4674
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4637..4642
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /note="hexamer: ATTAAA"
     polyA_site      4674
                     /gene="EPHB1"
                     /gene_synonym="ELK; EPHT2; Hek6; NET"
                     /note="major polyA site"
ORIGIN      
        1 agtctggccg gctccgtcct cccgtaggct ccgctgtagc tagcaatgtg acaccaggac
       61 gcactcgctc tcgcgcgctc tcccaggctc gttctccctc gccctctctc tctcacacac
      121 gcacgcacac acccacctct cccataaaca cacacacaca catgcacacc cacacccacg
      181 cgcgcccgca ccgccccacg cgcacacact cctgcccacg cccacgcagc gctccgggaa
      241 gtccggtccg ggcgagagcg cgaaaggata ccgagaagcc acccgcggag agcgcagcgg
      301 cgccctggga cgcggcgctc tcccggcgct gctgcctcgg cttggtctcg gcctgcgggc
      361 cgtcggccgg cgatggccct ggattatcta ctactgctcc tcctggcatc cgcagtggct
      421 gcgatggaag aaacgttaat ggacaccaga acggctactg cagagctggg ctggacggcc
      481 aatcctgcgt ccgggtggga agaagtcagt ggctacgatg aaaacctgaa caccatccgc
      541 acctaccagg tgtgcaatgt cttcgagccc aaccagaaca attggctgct caccaccttc
      601 atcaaccggc ggggggccca tcgcatctac acagagatgc gcttcactgt gagagactgc
      661 agcagcctcc ctaatgtccc aggatcctgc aaggagacct tcaacttgta ttactatgag
      721 actgactctg tcattgccac caagaagtca gccttctggt ctgaggcccc ctacctcaaa
      781 gtagacacca ttgctgcaga tgagagcttc tcccaggtgg actttggggg aaggctgatg
      841 aaggtaaaca cagaagtcag gagctttggg cctcttactc ggaatggttt ttacctcgct
      901 tttcaggatt atggagcctg tatgtctctt ctttctgtcc gtgtcttctt caaaaagtgt
      961 cccagcattg tgcaaaattt tgcagtgttt ccagagacta tgacaggggc agagagcaca
     1021 tctctggtga ttgctcgggg cacatgcatc cccaacgcag aggaagtgga cgtgcccatc
     1081 aaactctact gcaacgggga tggggaatgg atggtgccta ttgggcgatg cacctgcaag
     1141 cctggctatg agcctgagaa cagcgtggca tgcaaggctt gccctgcagg gacattcaag
     1201 gccagccagg aagctgaagg ctgctcccac tgcccctcca acagccgctc ccctgcagag
     1261 gcgtctccca tctgcacctg tcggaccggt tattaccgag cggactttga ccctccagaa
     1321 gtggcatgca ctagcgtccc atcaggtccc cgcaatgtta tctccatcgt caatgagacg
     1381 tccatcattc tggagtggca ccctccaagg gagacaggtg ggcgggatga tgtgacctac
     1441 aacatcatct gcaaaaagtg ccgggcagac cgccggagct gctcccgctg tgacgacaat
     1501 gtggagtttg tgcccaggca gctgggcctg acggagtgcc gcgtctccat cagcagcctg
     1561 tgggcccaca ccccctacac ctttgacatc caggccatca atggagtctc cagcaagagt
     1621 cccttccccc cacagcacgt ctctgtcaac atcaccacaa accaagccgc cccctccacc
     1681 gttcccatca tgcaccaagt cagtgccact atgaggagca tcaccttgtc atggccacag
     1741 ccggagcagc ccaatggcat catcctggac tatgagatcc ggtactatga gaaggaacac
     1801 aatgagttca actcctccat ggccaggagt cagaccaaca cagcaaggat tgatgggctg
     1861 cggcctggca tggtatatgt ggtacaggtg cgtgcccgca ctgttgctgg ctacggcaag
     1921 ttcagtggca agatgtgctt ccagactctg actgacgatg attacaagtc agagctgagg
     1981 gagcagctgc ccctgattgc tggctcggca gcggccgggg tcgtgttcgt tgtgtccttg
     2041 gtggccatct ctatcgtctg tagcaggaaa cgggcttata gcaaagaggc tgtgtacagc
     2101 gataagctcc agcattacag cacaggccga ggctccccag ggatgaagat ctacattgac
     2161 cccttcactt acgaggatcc caacgaagct gtccgggagt ttgccaagga gattgatgta
     2221 tcttttgtga aaattgaaga ggtcatcgga gcaggggagt ttggagaagt gtacaagggg
     2281 cgtttgaaac tgccaggcaa gagggaaatc tacgtggcca tcaagaccct gaaggcaggg
     2341 tactcggaga agcagcgtcg ggactttctg agtgaggcga gcatcatggg ccagttcgac
     2401 catcctaaca tcattcgcct ggagggtgtg gtcaccaaga gtcggcctgt catgatcatc
     2461 acagagttca tggagaatgg tgcattggat tctttcctca ggcaaaatga cgggcagttc
     2521 accgtgatcc agcttgtggg tatgctcagg ggcatcgctg ctggcatgaa gtacctggct
     2581 gagatgaatt atgtgcatcg ggacctggct gctaggaaca ttctggtcaa cagtaacctg
     2641 gtgtgcaagg tgtccgactt tggcctctcc cgctacctcc aggatgacac ctcagatccc
     2701 acctacacca gctccttggg agggaagatc cctgtgagat ggacagctcc agaggccatc
     2761 gcctaccgca agttcacttc agccagcgac gtttggagct atgggatcgt catgtgggaa
     2821 gtcatgtcat ttggagagag accctattgg gatatgtcca accaagatgt catcaatgcc
     2881 atcgagcagg actaccggct gcccccaccc atggactgtc cagctgctct acaccagctc
     2941 atgctggact gttggcagaa ggaccggaac agccggcccc ggtttgcgga gattgtcaac
     3001 accctagata agatgatccg gaacccggca agtctcaaga ctgtggcaac catcaccgcc
     3061 gtgccttccc agcccctgct cgaccgctcc atcccagact tcacggcctt taccaccgtg
     3121 gatgactggc tcagcgccat caaaatggtc cagtacaggg acagcttcct cactgctggc
     3181 ttcacctccc tccagctggt cacccagatg acatcagaag acctcctgag aataggcatc
     3241 accttggcag gccatcagaa gaagatcctg aacagcattc attctatgag ggtccagata
     3301 agtcagtcac caacggcaat ggcatgagaa ctcttgtttc ttggggaagg agaggaggga
     3361 aaaggaccag ggtcaagggg gaccagaggt tgaccactgt ggaatgtact ggagagactg
     3421 gcttctcagc tgaggaatgc atttccatca gtgaagaatc aaccggacct gttgctagca
     3481 ggcaatctcc atttctcagt gacagaagca tgtttgagat gccgtgggaa accaaatata
     3541 taataataaa aatataaaaa ggtgatgttc aacagaagtg aagacaaaac aatatgcatc
     3601 aggagaacaa gagtaaaccc agctcccatt ctcagtgggc tgcagttgcc caaccacagg
     3661 aagaaaggga aggaggtaga gggaagaaac agaagcagtg ttccattttc ttcctcacca
     3721 atgacattct tttcttttct cctttcgtac tcctccctga gagtcccctc ccttctccca
     3781 cactcgtttc cctttgctca tgactcctgt agggaagttt cttcaaacaa aacccagctc
     3841 ctgagtctcc agatgttgtt ctgtcagttg ccaaaggact ttgctgacca ctgcatgggg
     3901 atccaaccaa ttcaattaat gtcttcatat tgaagaagag atgtaccttc aattgaaaac
     3961 ctcgtttttc ttttgtttgc attttctgca aaaaggaaaa agaaaccaca aattggggaa
     4021 aaaaaaagaa gaaaaacctg tttccgtgtg caaaagcaca catatgtatg tctgtgttat
     4081 aaaatgactg tgcttgttcg taacagatgc aaacaagaaa gaagaactgg gaagtctttg
     4141 tccctaggaa atccaaaggg gctggaatat ggtgttggtt tggctttctg gttggcccaa
     4201 tcggcctatt ggctcaatgg gaagagagga gagggagaaa aataaaatga aaggaaaaaa
     4261 aaaagtttgc aaattcagac aggaaacagg tgagtggttt gaattggatg cagtgtgggc
     4321 catcctggaa tgatactgac tgattaatta ttcctgataa catctcaaga aaaggagaag
     4381 gaaagtgttt ctggagaatg ttctttcaca tcactggaat ctgcaattca agaagtgaca
     4441 agggagaatt cttgctttac ctatggactg gcttaagccg tgtggcatcc gaggaatgtt
     4501 tcaaatgtgt ctgtgtttct ctttacattc cttgttgtac ctcattgttc aattcacttt
     4561 tgtaaattcc acctaacatt taattatttt aaatttctcc ttttacctta atctccttgc
     4621 taattttatc tgtctaatta aaaagagcag aagcatgtct gggtttacgt aaaa
//
