LOCUS       NM_020526               5019 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens EPH receptor A8 (EPHA8), transcript variant 1, mRNA.
ACCESSION   NM_020526
VERSION     NM_020526.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Wang,G.H., Ni,K., Gu,C., Huang,J., Chen,J., Wang,X.D. and Ni,Q.
  TITLE     EphA8 inhibits cell apoptosis via AKT signaling and is associated
            with poor prognosis in breast cancer
  JOURNAL   Oncol Rep 46 (2) (2021)
   PUBMED   34278497
  REMARK    GeneRIF: EphA8 inhibits cell apoptosis via AKT signaling and is
            associated with poor prognosis in breast cancer.
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Wang,Y., Zhou,N., Li,P., Wu,H., Wang,Q., Gao,X., Wang,X. and
            Huang,J.
  TITLE     EphA8 acts as an oncogene and contributes to poor prognosis in
            gastric cancer via regulation of ADAM10
  JOURNAL   J Cell Physiol 234 (11), 20408-20419 (2019)
   PUBMED   31026069
  REMARK    GeneRIF: EphA8 knockdown also decreased expression of the protease
            ADAM10
REFERENCE   3  (bases 1 to 5019)
  AUTHORS   Liu,L., Wang,X. and Ge,W.
  TITLE     EphA8 is a Prognostic Factor for Oral Tongue Squamous Cell
            Carcinoma
  JOURNAL   Med Sci Monit 24, 7213-7222 (2018)
   PUBMED   30300334
  REMARK    GeneRIF: Q-PCR and IHC staining revealed that EphA8 was highly
            expressed in OTSCC tissues, especially in advanced stage OTSCC
            tissues. Kaplan-Meier curve showed that high EphA8 expression was
            significantly associated with poor prognosis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5019)
  AUTHORS   Apellaniz-Ruiz,M., Tejero,H., Inglada-Perez,L., Sanchez-Barroso,L.,
            Gutierrez-Gutierrez,G., Calvo,I., Castelo,B., Redondo,A.,
            Garcia-Donas,J., Romero-Laorden,N., Sereno,M., Merino,M.,
            Curras-Freixes,M., Montero-Conde,C., Mancikova,V.,
            Avall-Lundqvist,E., Green,H., Al-Shahrour,F., Cascon,A., Robledo,M.
            and Rodriguez-Antona,C.
  TITLE     Targeted Sequencing Reveals Low-Frequency Variants in EPHA Genes as
            Markers of Paclitaxel-Induced Peripheral Neuropathy
  JOURNAL   Clin Cancer Res 23 (5), 1227-1235 (2017)
   PUBMED   27582484
  REMARK    GeneRIF: Gene-based analysis identified EPHA6 as the gene most
            significantly associated with paclitaxel-induced neuropathy...This
            first study sequencing EPHA genes revealed that low-frequency
            variants in EPHA6, EPHA5, and EPHA8 contribute to the
            susceptibility to paclitaxel-induced neuropathy
REFERENCE   5  (bases 1 to 5019)
  AUTHORS   Liu,X., Xu,Y., Jin,Q., Wang,W., Zhang,S., Wang,X., Zhang,Y., Xu,X.
            and Huang,J.
  TITLE     EphA8 is a prognostic marker for epithelial ovarian cancer
  JOURNAL   Oncotarget 7 (15), 20801-20809 (2016)
   PUBMED   26989075
  REMARK    GeneRIF: High EPHA8 expression is associated with epithelial
            ovarian cancer.
REFERENCE   6  (bases 1 to 5019)
  AUTHORS   .
  TITLE     Unified nomenclature for Eph family receptors and their ligands,
            the ephrins. Eph Nomenclature Committee
  JOURNAL   Cell 90 (3), 403-404 (1997)
   PUBMED   9267020
REFERENCE   7  (bases 1 to 5019)
  AUTHORS   Park,S., Frisen,J. and Barbacid,M.
  TITLE     Aberrant axonal projections in mice lacking EphA8 (Eek) tyrosine
            protein kinase receptors
  JOURNAL   EMBO J 16 (11), 3106-3114 (1997)
   PUBMED   9214628
REFERENCE   8  (bases 1 to 5019)
  AUTHORS   Park,S. and Sanchez,M.P.
  TITLE     The Eek receptor, a member of the Eph family of tyrosine protein
            kinases, can be activated by three different Eph family ligands
  JOURNAL   Oncogene 14 (5), 533-542 (1997)
   PUBMED   9053851
REFERENCE   9  (bases 1 to 5019)
  AUTHORS   Lemke,G.
  TITLE     A coherent nomenclature for Eph receptors and their ligands
  JOURNAL   Mol Cell Neurosci 9 (5-6), 331-332 (1997)
   PUBMED   9361271
REFERENCE   10 (bases 1 to 5019)
  AUTHORS   Chan,J. and Watt,V.M.
  TITLE     eek and erk, new members of the eph subclass of receptor
            protein-tyrosine kinases
  JOURNAL   Oncogene 6 (6), 1057-1061 (1991)
   PUBMED   1648701
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL035703.21, DR007053.1,
            BC072417.1 and AB040892.1.
            
            On Nov 22, 2018 this sequence version replaced NM_020526.4.
            
            Summary: This gene encodes a member of the ephrin receptor
            subfamily of the protein-tyrosine kinase family. EPH and
            EPH-related receptors have been implicated in mediating
            developmental events, particularly in the nervous system. Receptors
            in the EPH subfamily typically have a single kinase domain and an
            extracellular region containing a Cys-rich domain and 2 fibronectin
            type III repeats. The ephrin receptors are divided into 2 groups
            based on the similarity of their extracellular domain sequences and
            their affinities for binding ephrin-A and ephrin-B ligands. The
            protein encoded by this gene functions as a receptor for ephrin A2,
            A3 and A5 and plays a role in short-range contact-mediated axonal
            guidance during development of the mammalian nervous system.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA2158188
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000166244.8/ ENSP00000166244.3
            RefSeq Select criteria    :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                AL035703.21        65783-65795         c
            14-22               DR007053.1         7-15
            23-1462             BC072417.1         1-1440
            1463-1980           AB040892.1         862-1379
            1981-2077           AL035703.21        31808-31904         c
            2078-3195           AB040892.1         1477-2594
            3196-3744           AL035703.21        26964-27512         c
            3745-5018           AB040892.1         3144-4417
            5019-5019           AL035703.21        25689-25689         c
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.12"
     gene            1..5019
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /note="EPH receptor A8"
                     /db_xref="GeneID:2046"
                     /db_xref="HGNC:HGNC:3391"
                     /db_xref="MIM:176945"
     exon            1..241
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     CDS             148..3165
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /EC_number="2.7.10.1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; protein-tyrosine kinase; hydroxyaryl-protein
                     kinase; tyrosylprotein kinase; ephrin type-A receptor 8;
                     tyrosine-protein kinase receptor EEK; EPH- and ELK-related
                     tyrosine kinase; EPH-like kinase 3; EPH- and ELK-related
                     kinase"
                     /codon_start=1
                     /product="ephrin type-A receptor 8 isoform 1 precursor"
                     /protein_id="NP_065387.1"
                     /db_xref="CCDS:CCDS225.1"
                     /db_xref="GeneID:2046"
                     /db_xref="HGNC:HGNC:3391"
                     /db_xref="MIM:176945"
                     /translation="MAPARGRLPPALWVVTAAAAAATCVSAARGEVNLLDTSTIHGDW
                     GWLTYPAHGWDSINEVDESFQPIHTYQVCNVMSPNQNNWLRTSWVPRDGARRVYAEIK
                     FTLRDCNSMPGVLGTCKETFNLYYLESDRDLGASTQESQFLKIDTIAADESFTGADLG
                     VRRLKLNTEVRSVGPLSKRGFYLAFQDIGACLAILSLRIYYKKCPAMVRNLAAFSEAV
                     TGADSSSLVEVRGQCVRHSEERDTPKMYCSAEGEWLVPIGKCVCSAGYEERRDACVAC
                     ELGFYKSAPGDQLCARCPPHSHSAAPAAQACHCDLSYYRAALDPPSSACTRPPSAPVN
                     LISSVNGTSVTLEWAPPLDPGGRSDITYNAVCRRCPWALSRCEACGSGTRFVPQQTSL
                     VQASLLVANLLAHMNYSFWIEAVNGVSDLSPEPRRAAVVNITTNQAAPSQVVVIRQER
                     AGQTSVSLLWQEPEQPNGIILEYEIKYYEKDKEMQSYSTLKAVTTRATVSGLKPGTRY
                     VFQVRARTSAGCGRFSQAMEVETGKPRPRYDTRTIVWICLTLITGLVVLLLLLICKKR
                     HCGYSKAFQDSDEEKMHYQNGQAPPPVFLPLHHPPGKLPEPQFYAEPHTYEEPGRAGR
                     SFTREIEASRIHIEKIIGSGDSGEVCYGRLRVPGQRDVPVAIKALKAGYTERQRRDFL
                     SEASIMGQFDHPNIIRLEGVVTRGRLAMIVTEYMENGSLDTFLRTHDGQFTIMQLVGM
                     LRGVGAGMRYLSDLGYVHRDLAARNVLVDSNLVCKVSDFGLSRVLEDDPDAAYTTTGG
                     KIPIRWTAPEAIAFRTFSSASDVWSFGVVMWEVLAYGERPYWNMTNRDVISSVEEGYR
                     LPAPMGCPHALHQLMLDCWHKDRAQRPRFSQIVSVLDALIRSPESLRATATVSRCPPP
                     AFVRSCFDLRGGSGGGGGLTVGDWLDSIRMGRYRDHFAAGGYSSLGMVLRMNAQDVRA
                     LGITLMGHQKKILGSIQTMRAQLTSTQGPRRHL"
     exon            242..306
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     exon            307..970
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1126
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1462
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1587
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1750
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..1844
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..1912
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1913..2077
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2078..2263
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2264..2325
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2326..2535
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2536..2685
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2686..2876
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2877..3050
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     exon            3051..5019
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4998..5003
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /note="hexamer: AATAAA"
     polyA_site      5019
                     /gene="EPHA8"
                     /gene_synonym="EEK; EK3; HEK3"
                     /note="major polyA site"
ORIGIN      
        1 gtgtgcgctg ggagccgcgt gtgcgccggg gtgtgcgccc ggccgggtgt gcggagagcg
       61 agggagcgcg ctccctcccg acgcgcgggc cgcagcggcc aagcccgagg gtgcgtggcg
      121 cccccgcccg cccggcccgg cccggccatg gcccccgccc ggggccgcct gccccctgcg
      181 ctctgggtcg tcacggccgc ggcggcggcg gccacctgcg tgtccgcggc gcgcggcgaa
      241 gtgaatttgc tggacacgtc gaccatccac ggggactggg gctggctcac gtatccggct
      301 catgggtggg actccatcaa cgaggtggac gagtccttcc agcccatcca cacgtaccag
      361 gtttgcaacg tcatgagccc caaccagaac aactggctgc gcacgagctg ggtcccccga
      421 gacggcgccc ggcgcgtcta tgctgagatc aagtttaccc tgcgcgactg caacagcatg
      481 cctggtgtgc tgggcacctg caaggagacc ttcaacctct actacctgga gtcggaccgc
      541 gacctggggg ccagcacaca agaaagccag ttcctcaaaa tcgacaccat tgcggccgac
      601 gagagcttca caggtgccga ccttggtgtg cggcgtctca agctcaacac ggaggtgcgc
      661 agtgtgggtc ccctcagcaa gcgcggcttc tacctggcct tccaggacat aggtgcctgc
      721 ctggccatcc tctctctccg catctactat aagaagtgcc ctgccatggt gcgcaatctg
      781 gctgccttct cggaggcagt gacgggggcc gactcgtcct cactggtgga ggtgaggggc
      841 cagtgcgtgc ggcactcaga ggagcgggac acacccaaga tgtactgcag cgcggagggc
      901 gagtggctcg tgcccatcgg caaatgcgtg tgcagtgccg gctacgagga gcggcgggat
      961 gcctgtgtgg cctgtgagct gggcttctac aagtcagccc ctggggacca gctgtgtgcc
     1021 cgctgccctc cccacagcca ctccgcagct ccagccgccc aagcctgcca ctgtgacctc
     1081 agctactacc gtgcagccct ggacccgccg tcctcagcct gcacccggcc accctcggca
     1141 ccagtgaacc tgatctccag tgtgaatggg acatcagtga ctctggagtg ggcccctccc
     1201 ctggacccag gtggccgcag tgacatcacc tacaatgccg tgtgccgccg ctgcccctgg
     1261 gcactgagcc gctgcgaggc atgtgggagc ggcacccgct ttgtgcccca gcagacaagc
     1321 ctggtgcagg ccagcctgct ggtggccaac ctgctggccc acatgaacta ctccttctgg
     1381 atcgaggccg tcaatggcgt gtccgacctg agccccgagc cccgccgggc cgctgtggtc
     1441 aacatcacca cgaaccaggc agccccgtcc caggtggtgg tgatccgtca agagcgggcg
     1501 gggcagacca gcgtctcgct gctgtggcag gagcccgagc agccgaacgg catcatcctg
     1561 gagtatgaga tcaagtacta cgagaaggac aaggagatgc agagctactc caccctcaag
     1621 gccgtcacca ccagagccac cgtctccggc ctcaagccgg gcacccgcta cgtgttccag
     1681 gtccgagccc gcacctcagc aggctgtggc cgcttcagcc aggccatgga ggtggagacc
     1741 gggaaacccc ggccccgcta tgacaccagg accattgtct ggatctgcct gacgctcatc
     1801 acgggcctgg tggtgcttct gctcctgctc atctgcaaga agaggcactg tggctacagc
     1861 aaggccttcc aggactcgga cgaggagaag atgcactatc agaatggaca ggcaccccca
     1921 cctgtcttcc tgcctctgca tcaccccccg ggaaagctcc cagagcccca gttctatgcg
     1981 gaaccccaca cctacgagga gccaggccgg gcgggccgca gtttcactcg ggagatcgag
     2041 gcctctagga tccacatcga gaaaatcatc ggctctggag actccgggga agtctgctac
     2101 gggaggctgc gggtgccagg gcagcgggat gtgcccgtgg ccatcaaggc cctcaaagcc
     2161 ggctacacgg agagacagag gcgggacttc ctgagcgagg cgtccatcat ggggcaattc
     2221 gaccatccca acatcatccg cctcgagggt gtcgtcaccc gtggccgcct ggcaatgatt
     2281 gtgactgagt acatggagaa cggctctctg gacaccttcc tgaggaccca cgacgggcag
     2341 ttcaccatca tgcagctggt gggcatgctg agaggagtgg gtgccggcat gcgctacctc
     2401 tcagacctgg gctatgtcca ccgagacctg gccgcccgca acgtcctggt tgacagcaac
     2461 ctggtctgca aggtgtctga cttcgggctc tcacgggtgc tggaggacga cccggatgct
     2521 gcctacacca ccacgggcgg gaagatcccc atccgctgga cggccccaga ggccatcgcc
     2581 ttccgcacct tctcctcggc cagcgacgtg tggagcttcg gcgtggtcat gtgggaggtg
     2641 ctggcctatg gggagcggcc ctactggaac atgaccaacc gggatgtcat cagctctgtg
     2701 gaggaggggt accgcctgcc cgcacccatg ggctgccccc acgccctgca ccagctcatg
     2761 ctcgactgtt ggcacaagga ccgggcgcag cggcctcgct tctcccagat tgtcagtgtc
     2821 ctcgatgcgc tcatccgcag ccctgagagt ctcagggcca ccgccacagt cagcaggtgc
     2881 ccaccccctg ccttcgtccg gagctgcttt gacctccgag ggggcagcgg tggcggtggg
     2941 ggcctcaccg tgggggactg gctggactcc atccgcatgg gccggtaccg agaccacttc
     3001 gctgcgggcg gatactcctc tctgggcatg gtgctacgca tgaacgccca ggacgtgcgc
     3061 gccctgggca tcaccctcat gggccaccag aagaagatcc tgggcagcat tcagaccatg
     3121 cgggcccagc tgaccagcac ccaggggccc cgccggcacc tctgatgtac agccagcagg
     3181 gcccaggcag ccaccaagcc caccccaggt catgccagcg gcagaggacg tgaggggctg
     3241 gcagcaggca gggcggcccc aggcctctgc cctcctctca ggtgctggag gagctgaagg
     3301 cttcgccaca ggacctggag ttatcagggg tcaggcgcct gggaaggggc ctttggtggc
     3361 caccctggtg aggacacctg tcccccaggg caggcacctt ctcttttcca gagcctgggg
     3421 cctccacgtc acagagtcca acagggacat cactcgcctg cctctgtgtg cgtgcatgtg
     3481 tgtgtgtggt ggggggtgtt ctcacaaggt catgggatct catgtgaaca gtgtgtgatc
     3541 aagtgtgtcc accccttcgg gtctcagcat ggacgtgtgc atgttatgag cgtgtgctta
     3601 tccgttaagg ctggaggcac atgtgggtga tggtggatga tgtgtcatga atgaggaggt
     3661 gtgtgagcag ggaactcagt gtgacaccgc caggtccagc acccatgggg gcgggggaga
     3721 ggctcacccc tacgtccccc cacacctgga ggctggagcc aggggccact tctgaactgc
     3781 accagcacca ggcccaccct cgtctctgcc tgggtgagcc caccccggct gtatctcagg
     3841 tctgggtcct ccctctagcc gaggaggcca cctgcagcct ccacccggct ctcaccgctg
     3901 cttcaacagg aaaacagggt tcccggtcag tccggctggc cgccttcatg gaggcatcat
     3961 ggcagagcac atgagatgtc ctcagctggg cttggctgcc tggccagggc cgggggctca
     4021 gcagcctcct ctagcctctg atgccttccc tccacggccc aggtctcctc actcaaagtc
     4081 ccttcgccaa cctttcaatg cccagccctg acacctgccc tttgtccccc aggcctagga
     4141 tcagggacca gaggatccta tcttctcagc acccagccca ccccttcctg tagcatatgg
     4201 ggagactaag gcctggagag aggggtgatg ccccgtccca ggttgcactg caaccaagtg
     4261 tcagagtcgg ggctccggcc tcctgccaag gctcttgtcc ccatacacca tcccacaagg
     4321 gcgctggggg tggaagtgcc ctggaagccc ctcccctctc acactgacct ccccccttac
     4381 ggcccaccag ggtatgtaaa tatctctttt ctaccatgtc agaatatttt ttcctcactc
     4441 ctgacaatgc aaaaatggtc ttcaaagcac ataaaaagca cccagggtga gaaagcccca
     4501 tcccgggggc cgttggcagg cagggaagca ggaaccccac cgtgtgcccc ctgccagccc
     4561 cagagggagt ggcgagccca gctgcccagc cctgcccccc ctccccatag ccagcacagc
     4621 tatcccgcgg ggacaccagc actgagcccc ctctccctcc tgcaataatt cggggagtct
     4681 cagccccatc caggtgccgc ggccagctct ctacacctct atatattata ttactatata
     4741 gccgagctgt tcttccttcc tatggaagtc ggaaacatgg tcagaacacg atctgggggg
     4801 gggatcctgt cttcctcccc accccacccc actcttaccc aatttctggg ctctggatcc
     4861 tcacagtcat ggaggcaccg tgggcctggc acttgcaaaa gtgtggcccc tcactctagt
     4921 gtgtggtccc tctcagggtc ctggggatct gcctctctgt ggtctccatc ctgactcttg
     4981 aacttaccca caataagaat aaattctgcc tcatctttg
//
