LOCUS       NM_001270989            2681 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens epithelial mitogen (EPGN), transcript variant 1, mRNA.
ACCESSION   NM_001270989
VERSION     NM_001270989.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Freed,D.M., Bessman,N.J., Kiyatkin,A., Salazar-Cavazos,E.,
            Byrne,P.O., Moore,J.O., Valley,C.C., Ferguson,K.M., Leahy,D.J.,
            Lidke,D.S. and Lemmon,M.A.
  TITLE     EGFR Ligands Differentially Stabilize Receptor Dimers to Specify
            Signaling Kinetics
  JOURNAL   Cell 171 (3), 683-695 (2017)
   PUBMED   28988771
  REMARK    GeneRIF: Study shows how the EGFR ligands epiregulin (EREG) and
            epigen (EPGN) stabilize different dimeric conformations of the EGFR
            extracellular region. Results reveal how responses to different
            EGFR ligands are defined by receptor dimerization strength and
            signaling dynamics. These findings have broad implications for
            understanding receptor tyrosine kinase (RTK) signaling specificity.
REFERENCE   3  (bases 1 to 2681)
  AUTHORS   Kennedy,R.B., Ovsyannikova,I.G., Pankratz,V.S., Haralambieva,I.H.,
            Vierkant,R.A. and Poland,G.A.
  TITLE     Genome-wide analysis of polymorphisms associated with cytokine
            responses in smallpox vaccine recipients
  JOURNAL   Hum Genet 131 (9), 1403-1421 (2012)
   PUBMED   22610502
REFERENCE   4  (bases 1 to 2681)
  AUTHORS   Wilson,K.J., Mill,C., Lambert,S., Buchman,J., Wilson,T.R.,
            Hernandez-Gordillo,V., Gallo,R.M., Ades,L.M., Settleman,J. and
            Riese,D.J. 2nd.
  TITLE     EGFR ligands exhibit functional differences in models of paracrine
            and autocrine signaling
  JOURNAL   Growth Factors 30 (2), 107-116 (2012)
   PUBMED   22260327
  REMARK    Review article
REFERENCE   5  (bases 1 to 2681)
  AUTHORS   Sahin,U. and Blobel,C.P.
  TITLE     Ectodomain shedding of the EGF-receptor ligand epigen is mediated
            by ADAM17
  JOURNAL   FEBS Lett 581 (1), 41-44 (2007)
   PUBMED   17169360
REFERENCE   6  (bases 1 to 2681)
  AUTHORS   Wang,A.G., Yoon,S.Y., Oh,J.H., Jeon,Y.J., Kim,M., Kim,J.M.,
            Byun,S.S., Yang,J.O., Kim,J.H., Kim,D.G., Yeom,Y.I., Yoo,H.S.,
            Kim,Y.S. and Kim,N.S.
  TITLE     Identification of intrahepatic cholangiocarcinoma related genes by
            comparison with normal liver tissues using expressed sequence tags
  JOURNAL   Biochem Biophys Res Commun 345 (3), 1022-1032 (2006)
   PUBMED   16712791
REFERENCE   7  (bases 1 to 2681)
  AUTHORS   Amsellem-Ouazana,D., Bieche,I., Tozlu,S., Botto,H., Debre,B. and
            Lidereau,R.
  TITLE     Gene expression profiling of ERBB receptors and ligands in human
            transitional cell carcinoma of the bladder
  JOURNAL   J Urol 175 (3 Pt 1), 1127-1132 (2006)
   PUBMED   16469638
REFERENCE   8  (bases 1 to 2681)
  AUTHORS   Kochupurakkal,B.S., Harari,D., Di-Segni,A., Maik-Rachline,G.,
            Lyass,L., Gur,G., Kerber,G., Citri,A., Lavi,S., Eilam,R.,
            Chalifa-Caspi,V., Eshhar,Z., Pikarsky,E., Pinkas-Kramarski,R.,
            Bacus,S.S. and Yarden,Y.
  TITLE     Epigen, the last ligand of ErbB receptors, reveals intricate
            relationships between affinity and mitogenicity
  JOURNAL   J Biol Chem 280 (9), 8503-8512 (2005)
   PUBMED   15611079
  REMARK    GeneRIF: EPG is an EGF-like growth factor and a low affinity ligand
REFERENCE   9  (bases 1 to 2681)
  AUTHORS   Strachan,L., Murison,J.G., Prestidge,R.L., Sleeman,M.A.,
            Watson,J.D. and Kumble,K.D.
  TITLE     Cloning and biological activity of epigen, a novel member of the
            epidermal growth factor superfamily
  JOURNAL   J Biol Chem 276 (21), 18265-18271 (2001)
   PUBMED   11278323
REFERENCE   10 (bases 1 to 2681)
  AUTHORS   .
  TITLE     Training and utilization of auxiliary personnel for rural health
            teams in developing countries. Report of a WHO Expert Committee
  JOURNAL   World Health Organ Tech Rep Ser (633), 1-35 (1979)
   PUBMED   112783
  REMARK    GeneRIF: cloning of a related gene in mouse
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CB150835.1, AK289455.1 and
            AC097470.3.
            
            On May 17, 2019 this sequence version replaced NM_001270989.1.
            
            Summary: The protein encoded by this gene is a member of the
            epidermal growth factor family. Members of this family are ligands
            for the epidermal growth factor receptor and play a role in cell
            survival, proliferation and migration. This protein has been
            reported to have high mitogenic activity but low affinity for its
            receptor. Expression of this transcript and protein have been
            reported in cancer specimens of the breast, bladder, and prostate.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Aug 2012].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189658.123526.1,
                                           SRR5189661.140394.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA2147975
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000413830.6/ ENSP00000411898.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 CB150835.1         1-3
            4-1199              AK289455.1         2-1197
            1200-2681           AC097470.3         94788-96269
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q13.3"
     gene            1..2681
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
                     /note="epithelial mitogen"
                     /db_xref="GeneID:255324"
                     /db_xref="HGNC:HGNC:17470"
                     /db_xref="MIM:618717"
     exon            1..107
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..529
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; epigen; epithelial mitogen homolog"
                     /codon_start=1
                     /product="epigen isoform 1 precursor"
                     /protein_id="NP_001257918.1"
                     /db_xref="CCDS:CCDS59478.1"
                     /db_xref="GeneID:255324"
                     /db_xref="HGNC:HGNC:17470"
                     /db_xref="MIM:618717"
                     /translation="MALGVPISVYLLFNAMTALTEEAAVTVTPPITAQQGNWTVNKTE
                     ADNIEGPIALKFSHLCLEDHNSYCINGACAFHHELEKAICRCFTGYTGERCEHLTLTS
                     YAVDSYEKYIAIGIGVGLLLSGFLVIFYCYIRKRCLKLKSPYNVCSGERRPL"
     exon            108..197
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
                     /inference="alignment:Splign:2.1.0"
     exon            198..318
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
                     /inference="alignment:Splign:2.1.0"
     exon            319..471
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
                     /inference="alignment:Splign:2.1.0"
     exon            472..2681
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
                     /inference="alignment:Splign:2.1.0"
     regulatory      864..869
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
                     /note="hexamer: AATAAT"
     polyA_site      889
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
                     /note="major polyA site"
     regulatory      1185..1190
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
                     /note="hexamer: ACTAAA"
     polyA_site      1208
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
     regulatory      1263..1268
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
                     /note="hexamer: TATAAA"
     polyA_site      1281
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
     regulatory      1383..1388
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
                     /note="hexamer: AATAAA"
     polyA_site      1423
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
     regulatory      2155..2160
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
                     /note="hexamer: TATAAA"
     regulatory      2163..2168
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
                     /note="hexamer: ATTAAA"
     polyA_site      2174
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
     polyA_site      2186
                     /gene="EPGN"
                     /gene_synonym="ALGV3072; EPG; PRO9904"
ORIGIN      
        1 aataaaaacc ttccacccgt cagtctagaa ggataagaga aagaaagtta agcaactaca
       61 ggaaatggct ttgggagttc caatatcagt ctatctttta ttcaacgcaa tgacagcact
      121 gaccgaagag gcagccgtga ctgtaacacc tccaatcaca gcccagcaag gtaactggac
      181 agttaacaaa acagaagctg acaacataga aggacccata gccttgaagt tctcacacct
      241 ttgcctggaa gatcataaca gttactgcat caacggtgct tgtgcattcc accatgagct
      301 agagaaagcc atctgcaggt gttttactgg ttatactgga gaaaggtgtg agcacttgac
      361 tttaacttca tatgctgtgg attcttatga aaaatacatt gcaattggga ttggtgttgg
      421 attactatta agtggttttc ttgttatttt ttactgctat ataagaaaga ggtgtctaaa
      481 attgaaatcg ccttacaatg tctgttctgg agaaagacga ccactgtgag gcctttgtga
      541 agaattttca tcaaggcatc tgtagagatc agtgagccca aaattaaagt tttcagatga
      601 aacaacaaaa cttgtcaagc tgactagact cgaaaataat gaaagttggg atcacaatga
      661 aatgagaaga taaaattcag cgttggcctt tagactttgc catccttaag gagtgatgga
      721 agccaagtga acaagcctca gtgacacaag tcaaattcat agtttcactc tgggtttttt
      781 gttgttgtgt ggttattatt ctcactacag aaagactgag tttcatgctc ctggctatgt
      841 cagatgtgaa ttttcatggg aataataatc aaccttgcag caagccaaag caatgcctcg
      901 cttgggttct tcatgttctt actacccagc gttttttacc acctagatgg gcctctctaa
      961 gtctatttgc tcaatgaacc ttatcccaaa cttgttgttt tcatggtgtc tgaaagaatg
     1021 tgatgtcagc ttttagaatg aaatcagtgt taaggtacct ccagtgaacc aaacatgtgt
     1081 cttaaatcta agccactgaa aacagaaggg aatgtccaag gcaaatacaa atcatacaca
     1141 gcttgtaaca acatacagcc tttctatgtg aaataatgga ataaactaaa gagtttctga
     1201 agactgaagc tatctggata tcaagtctgg agtaggcaaa gcatttccat ttctacatgg
     1261 attataaaac tttgtgttgg actcattggt ccctaatgct tttgttcatc acttcttcca
     1321 gttatcagtg gaattacctg gtgaccattc atttggaccg aaatcctgga agtctcctac
     1381 tgaataaaag tctacaattg gccctaaaat agaaactgaa aaacaggaca tagaattttt
     1441 tcaccagacc acagcatgtg gaaactttct ttatcatttt tgaacacttg ttaacagatt
     1501 tgcacataga gggagagaaa aaaaatggag taacagtcaa aataataata atcagtatcc
     1561 aggccaggcg tggtggttca cgcctgtaat cctagcactt tgggaggccg aggcggatgg
     1621 agcacctgag gtcaggagtt tgagaccagc ctggccaaca tggtgaaacc ctgtctctac
     1681 taaaaataca aaaattagct gggcgtggtg gtgcatgcct gtaatcccag ctactccaga
     1741 ggctgacaca ggacaatcac ttgaacccag gaggcagagg ttgtagtgag ccgagatggc
     1801 aaccctgcac tccaacctgg gtgacaagag cgaaactcca tctcaaaaaa aaaaaaaaaa
     1861 atcagtatcc ttcctcttag ttatatttct gccacttaat tcaaacagtc atatgcaggt
     1921 cgcttaattt atttgtgctt ttgtttcatc ttctacaagg ccctcttagc tctaaaactt
     1981 gacagtggaa taaggaaatg tttttccaaa tctgcattgc cggtgagatc ctcaacatca
     2041 gcatgttgag atggacctca accccacctc taaccctgaa acacactact cgatattatc
     2101 ttaggtatgt tttagggttt agtttgtaaa ataataattt atttttgaag gaaatataaa
     2161 atattaaaga gtaataatag ctatcatttt ttaagattca atctaaaaca atggactctt
     2221 tttttttcca tttgtgatgt agataagcaa gacaattttg atcatgagtg gtgaaaagag
     2281 gatcaaactt gactattctt gcaatggcag tccagcaaca agcctttcat ttacattaaa
     2341 ttataacttt tcattcattc ctaaaccaaa cttaaaattc tgctttcctt tgagtagaag
     2401 gtatttaact tgttttgttt ttccttcaga aggaatttaa tgcaaacgga ttgcagtcag
     2461 cactttctga atgttttcac acagtatgca aagcttacat cataccaagg agtggagagt
     2521 tgaagtttcc tcccagtgac tccagtgaca gaccacacct agaaagcgtt tctcttcctg
     2581 agtatttcaa aaagatgtaa aagagctggg gagagtatgg gaagaaacaa tacaggattg
     2641 cctttaatta attaagaatt gcctcctgat aaaaggaaaa a
//
